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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:EML4-MTR (FusionGDB2 ID:26466) |
Fusion Gene Summary for EML4-MTR |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: EML4-MTR | Fusion gene ID: 26466 | Hgene | Tgene | Gene symbol | EML4 | MTR | Gene ID | 27436 | 4548 |
| Gene name | EMAP like 4 | 5-methyltetrahydrofolate-homocysteine methyltransferase | |
| Synonyms | C2orf2|ELP120|EMAP-4|EMAPL4|ROPP120 | HMAG|MS|cblG | |
| Cytomap | 2p21 | 1q43 | |
| Type of gene | protein-coding | protein-coding | |
| Description | echinoderm microtubule-associated protein-like 4echinoderm microtubule associated protein like 4restrictedly overexpressed proliferation-associated proteinropp 120 | methionine synthase5-methyltetrahydrofolate-homocysteine methyltransferase 1cobalamin-dependent methionine synthasevitamin-B12 dependent methionine synthase | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q9HC35 | Q9UBK8 | |
| Ensembl transtripts involved in fusion gene | ENST00000318522, ENST00000401738, ENST00000402711, ENST00000453191, ENST00000482660, | ENST00000366577, ENST00000418145, ENST00000470570, ENST00000535889, | |
| Fusion gene scores | * DoF score | 22 X 32 X 18=12672 | 5 X 5 X 2=50 |
| # samples | 43 | 5 | |
| ** MAII score | log2(43/12672*10)=-4.88116377049015 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(5/50*10)=0 | |
| Context | PubMed: EML4 [Title/Abstract] AND MTR [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | EML4(42528533)-MTR(237050229), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Fusion gene breakpoints across EML4 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across MTR (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChiTaRS5.0 | N/A | AX053571 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| ChiTaRS5.0 | N/A | BD345559 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
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Fusion Gene ORF analysis for EML4-MTR |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-intron | ENST00000318522 | ENST00000366577 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000318522 | ENST00000418145 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000318522 | ENST00000470570 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000318522 | ENST00000535889 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000401738 | ENST00000366577 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000401738 | ENST00000418145 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000401738 | ENST00000470570 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000401738 | ENST00000535889 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000402711 | ENST00000366577 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000402711 | ENST00000418145 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000402711 | ENST00000470570 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5CDS-intron | ENST00000402711 | ENST00000535889 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5UTR-intron | ENST00000453191 | ENST00000366577 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5UTR-intron | ENST00000453191 | ENST00000418145 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5UTR-intron | ENST00000453191 | ENST00000470570 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| 5UTR-intron | ENST00000453191 | ENST00000535889 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| intron-intron | ENST00000482660 | ENST00000366577 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| intron-intron | ENST00000482660 | ENST00000418145 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| intron-intron | ENST00000482660 | ENST00000470570 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| intron-intron | ENST00000482660 | ENST00000535889 | EML4 | chr2 | 42528533 | + | MTR | chr1 | 237050229 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for EML4-MTR |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for EML4-MTR |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:42528533/:237050229) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| EML4 | MTR |
| FUNCTION: Essential for the formation and stability of microtubules (MTs) (PubMed:16890222, PubMed:31409757). Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs (PubMed:25789526). Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression (PubMed:25789526). {ECO:0000269|PubMed:16890222, ECO:0000269|PubMed:25789526, ECO:0000269|PubMed:31409757}. | FUNCTION: Key enzyme in methionine and folate homeostasis responsible for the reactivation of methionine synthase (MTR/MS) activity by catalyzing the reductive methylation of MTR-bound cob(II)alamin (PubMed:17892308). Cobalamin (vitamin B12) forms a complex with MTR to serve as an intermediary in methyl transfer reactions that cycles between MTR-bound methylcob(III)alamin and MTR bound-cob(I)alamin forms, and occasional oxidative escape of the cob(I)alamin intermediate during the catalytic cycle leads to the inactive cob(II)alamin species (Probable). The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine (PubMed:27771510). Also necessary for the utilization of methyl groups from the folate cycle, thereby affecting transgenerational epigenetic inheritance (By similarity). Also acts as a molecular chaperone for methionine synthase by stabilizing apoMTR and incorporating methylcob(III)alamin into apoMTR to form the holoenzyme (PubMed:16769880). Also serves as an aquacob(III)alamin reductase by reducing aquacob(III)alamin to cob(II)alamin; this reduction leads to stimulation of the conversion of apoMTR and aquacob(III)alamin to MTR holoenzyme (PubMed:16769880). {ECO:0000250|UniProtKB:Q8C1A3, ECO:0000269|PubMed:16769880, ECO:0000269|PubMed:17892308, ECO:0000269|PubMed:27771510, ECO:0000305|PubMed:19243433}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for EML4-MTR |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for EML4-MTR |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for EML4-MTR |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for EML4-MTR |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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