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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:GAPDH-HSPB1 (FusionGDB2 ID:32382) |
Fusion Gene Summary for GAPDH-HSPB1 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: GAPDH-HSPB1 | Fusion gene ID: 32382 | Hgene | Tgene | Gene symbol | GAPDH | HSPB1 | Gene ID | 2597 | 3315 |
| Gene name | glyceraldehyde-3-phosphate dehydrogenase | heat shock protein family B (small) member 1 | |
| Synonyms | G3PD|GAPD|HEL-S-162eP | CMT2F|HEL-S-102|HMN2B|HS.76067|HSP27|HSP28|Hsp25|SRP27 | |
| Cytomap | 12p13.31 | 7q11.23 | |
| Type of gene | protein-coding | protein-coding | |
| Description | glyceraldehyde-3-phosphate dehydrogenaseOCAS, p38 componentOct1 coactivator in S phase, 38 Kd componentaging-associated gene 9 proteinepididymis secretory sperm binding protein Li 162ePpeptidyl-cysteine S-nitrosylase GAPDH | heat shock protein beta-128 kDa heat shock proteinepididymis secretory protein Li 102estrogen-regulated 24 kDa proteinheat shock 27 kDa proteinheat shock 27kD protein 1heat shock 27kDa protein 1stress-responsive protein 27 | |
| Modification date | 20200327 | 20200327 | |
| UniProtAcc | P04406 | Q9Y547 | |
| Ensembl transtripts involved in fusion gene | ENST00000229239, ENST00000396856, ENST00000396859, ENST00000396861, ENST00000396858, | ENST00000248553, ENST00000429938, | |
| Fusion gene scores | * DoF score | 45 X 34 X 13=19890 | 11 X 10 X 4=440 |
| # samples | 51 | 11 | |
| ** MAII score | log2(51/19890*10)=-5.28540221886225 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(11/440*10)=-2 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: GAPDH [Title/Abstract] AND HSPB1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | GAPDH(6647481)-HSPB1(75931861), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | GAPDH-HSPB1 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF. GAPDH-HSPB1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. GAPDH-HSPB1 seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | GAPDH | GO:0010951 | negative regulation of endopeptidase activity | 22832495 |
| Hgene | GAPDH | GO:0017148 | negative regulation of translation | 23071094 |
| Hgene | GAPDH | GO:0031640 | killing of cells of other organism | 22832495 |
| Hgene | GAPDH | GO:0050715 | positive regulation of cytokine secretion | 22832495 |
| Hgene | GAPDH | GO:0050832 | defense response to fungus | 22832495 |
| Hgene | GAPDH | GO:0051873 | killing by host of symbiont cells | 22832495 |
| Hgene | GAPDH | GO:0052501 | positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction | 22832495 |
| Hgene | GAPDH | GO:0061844 | antimicrobial humoral immune response mediated by antimicrobial peptide | 22832495 |
| Hgene | GAPDH | GO:0071346 | cellular response to interferon-gamma | 15479637 |
| Fusion gene breakpoints across GAPDH (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across HSPB1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | SKCM | TCGA-EE-A3AG-06A | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
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Fusion Gene ORF analysis for GAPDH-HSPB1 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 3UTR-3CDS | ENST00000229239 | ENST00000248553 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 3UTR-3CDS | ENST00000396856 | ENST00000248553 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 3UTR-3CDS | ENST00000396859 | ENST00000248553 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 3UTR-3CDS | ENST00000396861 | ENST00000248553 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 3UTR-intron | ENST00000229239 | ENST00000429938 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 3UTR-intron | ENST00000396856 | ENST00000429938 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 3UTR-intron | ENST00000396859 | ENST00000429938 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 3UTR-intron | ENST00000396861 | ENST00000429938 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| 5CDS-intron | ENST00000396858 | ENST00000429938 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| Frame-shift | ENST00000396858 | ENST00000248553 | GAPDH | chr12 | 6647481 | - | HSPB1 | chr7 | 75931861 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for GAPDH-HSPB1 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for GAPDH-HSPB1 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:6647481/:75931861) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| GAPDH | HSPB1 |
| FUNCTION: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. {ECO:0000250, ECO:0000269|PubMed:11724794, ECO:0000269|PubMed:23071094, ECO:0000269|PubMed:3170585}. | FUNCTION: Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumulation of GLI2 at the ciliary tip in response to activation of the SHH pathway, suggesting it is involved in the dynamic transport of SHH signaling molecules within the cilium. Not required for ciliary assembly. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation. Plays a role in the early development of the kidney. May be involved in the regulation of ureteric bud initiation (By similarity). {ECO:0000250|UniProtKB:Q9D6H2}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for GAPDH-HSPB1 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for GAPDH-HSPB1 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for GAPDH-HSPB1 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for GAPDH-HSPB1 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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