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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:AK5-USP33 (FusionGDB2 ID:3250)

Fusion Gene Summary for AK5-USP33

check button Fusion gene summary
Fusion gene informationFusion gene name: AK5-USP33
Fusion gene ID: 3250
HgeneTgene
Gene symbol

AK5

USP33

Gene ID

26289

23032

Gene nameadenylate kinase 5ubiquitin specific peptidase 33
SynonymsAK6VDU1
Cytomap

1p31.1

1p31.1

Type of geneprotein-codingprotein-coding
Descriptionadenylate kinase isoenzyme 5AK 5ATP-AMP transphosphorylase 5adenylate kinase 6ubiquitin carboxyl-terminal hydrolase 33VHL-interacting deubiquitinating enzyme 1deubiquitinating enzyme 33pVHL-interacting deubiquitinating enzyme 1ubiquitin thioesterase 33ubiquitin thiolesterase 33ubiquitin-specific-processing protease 33
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000344720, ENST00000354567, 
ENST00000478255, ENST00000317704, 
ENST00000370792, ENST00000528150, 
ENST00000357428, ENST00000370793, 
ENST00000370794, 
Fusion gene scores* DoF score5 X 5 X 3=7513 X 11 X 8=1144
# samples 516
** MAII scorelog2(5/75*10)=-0.584962500721156
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(16/1144*10)=-2.83794324189103
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: AK5 [Title/Abstract] AND USP33 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointAK5(78001723)-USP33(78167156), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneAK5

GO:0006165

nucleoside diphosphate phosphorylation

23416111

HgeneAK5

GO:0009142

nucleoside triphosphate biosynthetic process

23416111

TgeneUSP33

GO:0009267

cellular response to starvation

24056301

TgeneUSP33

GO:0016579

protein deubiquitination

11739384|19363159|19424180|23486064

TgeneUSP33

GO:0070536

protein K63-linked deubiquitination

19363159

TgeneUSP33

GO:0071108

protein K48-linked deubiquitination

19363159


check buttonFusion gene breakpoints across AK5 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across USP33 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4CESCTCGA-WL-A834-01AAK5chr1

78001723

-USP33chr1

78167156

-


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Fusion Gene ORF analysis for AK5-USP33

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000344720ENST00000370792AK5chr1

78001723

-USP33chr1

78167156

-
5CDS-intronENST00000344720ENST00000528150AK5chr1

78001723

-USP33chr1

78167156

-
5CDS-intronENST00000354567ENST00000370792AK5chr1

78001723

-USP33chr1

78167156

-
5CDS-intronENST00000354567ENST00000528150AK5chr1

78001723

-USP33chr1

78167156

-
5CDS-intronENST00000478255ENST00000370792AK5chr1

78001723

-USP33chr1

78167156

-
5CDS-intronENST00000478255ENST00000528150AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000344720ENST00000357428AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000344720ENST00000370793AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000344720ENST00000370794AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000354567ENST00000357428AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000354567ENST00000370793AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000354567ENST00000370794AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000478255ENST00000357428AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000478255ENST00000370793AK5chr1

78001723

-USP33chr1

78167156

-
In-frameENST00000478255ENST00000370794AK5chr1

78001723

-USP33chr1

78167156

-
intron-3CDSENST00000317704ENST00000357428AK5chr1

78001723

-USP33chr1

78167156

-
intron-3CDSENST00000317704ENST00000370793AK5chr1

78001723

-USP33chr1

78167156

-
intron-3CDSENST00000317704ENST00000370794AK5chr1

78001723

-USP33chr1

78167156

-
intron-intronENST00000317704ENST00000370792AK5chr1

78001723

-USP33chr1

78167156

-
intron-intronENST00000317704ENST00000528150AK5chr1

78001723

-USP33chr1

78167156

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000354567AK5chr178001723-ENST00000370794USP33chr178167156-354318831612212683
ENST00000354567AK5chr178001723-ENST00000357428USP33chr178167156-353918831612212683
ENST00000354567AK5chr178001723-ENST00000370793USP33chr178167156-353918831612212683
ENST00000344720AK5chr178001723-ENST00000370794USP33chr178167156-4228256810142897627
ENST00000344720AK5chr178001723-ENST00000357428USP33chr178167156-4224256810142897627
ENST00000344720AK5chr178001723-ENST00000370793USP33chr178167156-4224256810142897627
ENST00000478255AK5chr178001723-ENST00000370794USP33chr178167156-2086426249755168
ENST00000478255AK5chr178001723-ENST00000357428USP33chr178167156-2082426249755168
ENST00000478255AK5chr178001723-ENST00000370793USP33chr178167156-2082426249755168

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000354567ENST00000370794AK5chr178001723-USP33chr178167156-0.0009413340.99905866
ENST00000354567ENST00000357428AK5chr178001723-USP33chr178167156-0.0009610710.99903893
ENST00000354567ENST00000370793AK5chr178001723-USP33chr178167156-0.0009610710.99903893
ENST00000344720ENST00000370794AK5chr178001723-USP33chr178167156-0.0003221450.9996779
ENST00000344720ENST00000357428AK5chr178001723-USP33chr178167156-0.0003286530.99967134
ENST00000344720ENST00000370793AK5chr178001723-USP33chr178167156-0.0003286530.99967134
ENST00000478255ENST00000370794AK5chr178001723-USP33chr178167156-0.002205480.9977945
ENST00000478255ENST00000357428AK5chr178001723-USP33chr178167156-0.0023074840.9976926
ENST00000478255ENST00000370793AK5chr178001723-USP33chr178167156-0.0023074840.9976926

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Fusion Genomic Features for AK5-USP33


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for AK5-USP33


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:78001723/chr1:78167156)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314142_147514537.0Nucleotide bindingATP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314191_193514537.0Nucleotide bindingAMP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314219_222514537.0Nucleotide bindingAMP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314386_391514537.0Nucleotide bindingATP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314433_435514537.0Nucleotide bindingAMP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314462_465514537.0Nucleotide bindingAMP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314142_147540563.0Nucleotide bindingATP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314191_193540563.0Nucleotide bindingAMP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314219_222540563.0Nucleotide bindingAMP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314386_391540563.0Nucleotide bindingATP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314433_435540563.0Nucleotide bindingAMP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314462_465540563.0Nucleotide bindingAMP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314133_316514537.0RegionAdenylate kinase 1
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314162_193514537.0RegionNMP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314256_266514537.0RegionLID 1
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314406_435514537.0RegionNMP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314499_509514537.0RegionLID 2
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314133_316540563.0RegionAdenylate kinase 1
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314162_193540563.0RegionNMP 1
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314256_266540563.0RegionLID 1
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314406_435540563.0RegionNMP 2
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314499_509540563.0RegionLID 2
TgeneUSP33chr1:78001723chr1:78167156ENST00000370792022185_7150829.0DomainNote=USP
TgeneUSP33chr1:78001723chr1:78167156ENST00000370792022717_8100829.0DomainDUSP 1
TgeneUSP33chr1:78001723chr1:78167156ENST00000370792022818_9210829.0DomainDUSP 2
TgeneUSP33chr1:78001723chr1:78167156ENST000003707942024818_921802912.0DomainDUSP 2
TgeneUSP33chr1:78001723chr1:78167156ENST0000037079202259_1230829.0Zinc fingerUBP-type

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAK5chr1:78001723chr1:78167156ENST00000344720-1314377_559514537.0RegionAdenylate kinase 2
HgeneAK5chr1:78001723chr1:78167156ENST00000354567-1314377_559540563.0RegionAdenylate kinase 2
TgeneUSP33chr1:78001723chr1:78167156ENST000003574282024185_715833943.0DomainNote=USP
TgeneUSP33chr1:78001723chr1:78167156ENST000003574282024717_810833943.0DomainDUSP 1
TgeneUSP33chr1:78001723chr1:78167156ENST000003574282024818_921833943.0DomainDUSP 2
TgeneUSP33chr1:78001723chr1:78167156ENST000003707932125185_715833943.0DomainNote=USP
TgeneUSP33chr1:78001723chr1:78167156ENST000003707932125717_810833943.0DomainDUSP 1
TgeneUSP33chr1:78001723chr1:78167156ENST000003707932125818_921833943.0DomainDUSP 2
TgeneUSP33chr1:78001723chr1:78167156ENST000003707942024185_715802912.0DomainNote=USP
TgeneUSP33chr1:78001723chr1:78167156ENST000003707942024717_810802912.0DomainDUSP 1
TgeneUSP33chr1:78001723chr1:78167156ENST00000357428202459_123833943.0Zinc fingerUBP-type
TgeneUSP33chr1:78001723chr1:78167156ENST00000370793212559_123833943.0Zinc fingerUBP-type
TgeneUSP33chr1:78001723chr1:78167156ENST00000370794202459_123802912.0Zinc fingerUBP-type


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Fusion Gene Sequence for AK5-USP33


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>3250_3250_1_AK5-USP33_AK5_chr1_78001723_ENST00000344720_USP33_chr1_78167156_ENST00000357428_length(transcript)=4224nt_BP=2568nt
AGTCTTCCAGCAGGGTTCTGAAAGCAGCCTGCTCCCATCGCGCCCCTCGGATGTGGACACCCAGGGTGGGCTGCAGACCGCGGCCGGGCC
GCACTCTCTGGGGGCGAGGGCCAGGTCAGGTGGCTGCACTGTCGCCCAGCAGCGAGTAAAGACTCGAAACTGAGGGAGCCAGAGCCCTAG
TCACTCTGCTGGTCGAGGGTCGGAACCCCTGGGGGAAAGAGGAAATGTGGACGAAGGCAGCCCTTCCTGGAGACAGCTTGGAAGAGAGGG
ATGAAGATGCAAAAAGCAAAACCCAACCTATCAAGTGCCACCTCCCCGGTGACAGGCCCCCAGCCTTGCTCTGCGCTTTCGCTGAATGAA
CTCCTGAGCACGTTTTTCCCGTTGCCTAGGAAGGGTTGGGCCGCAGGTTTCGCCAGTGCTCGACAACCCACCTGGAGATAGCGGGTCGGG
GAGGGAGCTGCGAGGGGGGCGGACTCCTGTTGCTCAGTCTTCAGACCGCAGCACAGCATCATCTGCACCGGGACCCGGGAATGGGGGGAC
ACTTGGAGACTGAGAGAGAGGAGGCTCGAGGAATGAACCTTGGTCTGTTCTAAGCCACAACCCCTGTCTCAGGTCGTTTGGCTTCCTTTC
CTTCTCTTTGTATTTGAATTTAAAAACATTCCTGAATCTCAAGTGCTTTTGCTTGAAGTAGGGGGGAGGAGGAGAAAAGAATAGAAGCAA
GCCAGGCTTCCTAAGCGAGCTCTGAAAGGAGAAATCCATTCACTGCAAACCTCGTTCCCCATTTCTGAAAACGGATAGACTGATATCGTA
AACTAAAGGTCATGAGGGTTTTTCCCCCCGGTTTGGGGGATTTGTTCTGTTAAAAGGAATTTTAAAGCACCACTTGAGAAAATAATTTTG
CCTCAGTTTCCCTAAACTATCTTTAAAATGGGTTGCCATAATTACAGGTCTTCTTTTCTCTCACCTCTATAAGCCACCGTTTTCAGGTCA
TCTGAAGTCAAGATTTCCAGCCTTTTGAATGGACTGATGTGTTCTAAGCCCGAAGATCCAGTAGAATACTTGGAAAGTTGTTTACAAAAA
GTAAAGGAACTGGGTGGCTGTGACAAGGTGAAATGGGATACATTTGTAAGCCAGGAAAAGAAGACCTTACCTCCACTAAATGGAGGACAG
TCACGGAGATCCTTTCTAAGAAATGTAATGCCTGAAAACTCAAACTTTCCATATCGGCGGTATGACCGGCTCCCTCCAATCCATCAATTC
TCCATAGAAAGTGACACGGATCTCTCTGAGACTGCAGAGTTGATTGAGGAGTATGAGGTTTTTGATCCTACCAGACCTCGACCAAAAATC
ATTCTTGTTATAGGTGGTCCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTGCAGAACGATATGGATTCCAATACATTTCTGTGGGA
GAATTATTAAGAAAGAAGATCCACAGTACCAGCAGCAATAGGAAATGGAGTCTTATTGCCAAGATAATTACAACTGGAGAATTGGCCCCA
CAGGAAACAACAATTACAGAGATAAAGCAAAAATTGATGCAAATACCTGATGAAGAGGGCATTGTTATTGATGGATTTCCAAGAGATGTT
GCCCAGGCTCTATCTTTTGAGGACCAAATCTGTACCCCCGATTTGGTGGTATTCCTGGCTTGTGCTAATCAGAGACTCAAAGAAAGATTA
CTGAAGCGTGCAGAACAGCAGGGCCGACCAGACGACAATGTAAAAGCTACCCAAAGGAGACTAATGAACTTCAAGCAGAATGCTGCTCCA
TTGGTTAAATACTTCCAGGAAAAGGGGCTCATCATGACATTTGATGCCGACCGCGATGAGGATGAGGTGTTCTATGACATCAGCATGGCA
GTTGACAACAAGTTATTTCCAAACAAAGAGGCTGCAGCAGGTTCAAGTGACCTTGATCCTTCGATGATATTGGACACTGGAGAGATCATT
GATACAGGATCTGATTATGAAGATCAGGGTGATGACCAGTTAAATGTATTTGGAGAGGACACTATGGGAGGTTTCATGGAAGATTTGAGA
AAGTGTAAAATTATTTTCATAATTGGTGGTCCTGGCTCTGGCAAAGGCACACAGTGTGAAAAGCTGGTGGAAAAATATGGATTTACACAT
CTCTCAACTGGCGAGCTCCTGCGTGAGGAACTGGCATCAGAATCTGAAAGAAGCAAATTGATCAGAGACATTATGGAACGTGGAGACCTG
GTGCCCTCAGGCATCGTTTTGGAGCTCCTGAAGGAGGCCATGGTGGCCAGCCTCGGGGACACCAGGGGCTTCCTGATTGACGGCTATCCT
CGGGAGGTGAAGCAAGGGGAAGAGTTCGGACGCAGGATTGGAGACCCACAGTTGGTGATCTGTATGGACTGCTCGGCAGACACCATGACC
AACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGACCATCGCCAAGCGCCTAGAAGCCTACTACCGAGCG
TCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTT
TATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAG
ATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCT
ATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAAAAATTGAAGTA
GAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTGACATGTACACATGCGAAAACATT
CCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTCTTCTTAGTGGGCATTATGGAAGAATATATTAAAAT
GTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTTTCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTT
TGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCTC
GAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTAG
TCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGT
AATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATCCATAGTATCCTTTGTAGT
GTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTA
ATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCA
GGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTCTTTTTGAATGTATCAAGG
ATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAAACTTCAATTCCTGTAACC
TGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTTTTATTTTGAGAGCATGAAGTGTGGAATGTGTCACT
ACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGATAAAGTCTTTTTGAATAATATAGTATGCACTGCTA
TTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGATGTTGAATAGCATTCATG

>3250_3250_1_AK5-USP33_AK5_chr1_78001723_ENST00000344720_USP33_chr1_78167156_ENST00000357428_length(amino acids)=627AA_BP=518
MNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKWDTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDL
SETAELIEEYEVFDPTRPRPKIILVIGGPGSGKGTQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEI
KQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEK
GLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFII
GGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEE
FGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWF

--------------------------------------------------------------
>3250_3250_2_AK5-USP33_AK5_chr1_78001723_ENST00000344720_USP33_chr1_78167156_ENST00000370793_length(transcript)=4224nt_BP=2568nt
AGTCTTCCAGCAGGGTTCTGAAAGCAGCCTGCTCCCATCGCGCCCCTCGGATGTGGACACCCAGGGTGGGCTGCAGACCGCGGCCGGGCC
GCACTCTCTGGGGGCGAGGGCCAGGTCAGGTGGCTGCACTGTCGCCCAGCAGCGAGTAAAGACTCGAAACTGAGGGAGCCAGAGCCCTAG
TCACTCTGCTGGTCGAGGGTCGGAACCCCTGGGGGAAAGAGGAAATGTGGACGAAGGCAGCCCTTCCTGGAGACAGCTTGGAAGAGAGGG
ATGAAGATGCAAAAAGCAAAACCCAACCTATCAAGTGCCACCTCCCCGGTGACAGGCCCCCAGCCTTGCTCTGCGCTTTCGCTGAATGAA
CTCCTGAGCACGTTTTTCCCGTTGCCTAGGAAGGGTTGGGCCGCAGGTTTCGCCAGTGCTCGACAACCCACCTGGAGATAGCGGGTCGGG
GAGGGAGCTGCGAGGGGGGCGGACTCCTGTTGCTCAGTCTTCAGACCGCAGCACAGCATCATCTGCACCGGGACCCGGGAATGGGGGGAC
ACTTGGAGACTGAGAGAGAGGAGGCTCGAGGAATGAACCTTGGTCTGTTCTAAGCCACAACCCCTGTCTCAGGTCGTTTGGCTTCCTTTC
CTTCTCTTTGTATTTGAATTTAAAAACATTCCTGAATCTCAAGTGCTTTTGCTTGAAGTAGGGGGGAGGAGGAGAAAAGAATAGAAGCAA
GCCAGGCTTCCTAAGCGAGCTCTGAAAGGAGAAATCCATTCACTGCAAACCTCGTTCCCCATTTCTGAAAACGGATAGACTGATATCGTA
AACTAAAGGTCATGAGGGTTTTTCCCCCCGGTTTGGGGGATTTGTTCTGTTAAAAGGAATTTTAAAGCACCACTTGAGAAAATAATTTTG
CCTCAGTTTCCCTAAACTATCTTTAAAATGGGTTGCCATAATTACAGGTCTTCTTTTCTCTCACCTCTATAAGCCACCGTTTTCAGGTCA
TCTGAAGTCAAGATTTCCAGCCTTTTGAATGGACTGATGTGTTCTAAGCCCGAAGATCCAGTAGAATACTTGGAAAGTTGTTTACAAAAA
GTAAAGGAACTGGGTGGCTGTGACAAGGTGAAATGGGATACATTTGTAAGCCAGGAAAAGAAGACCTTACCTCCACTAAATGGAGGACAG
TCACGGAGATCCTTTCTAAGAAATGTAATGCCTGAAAACTCAAACTTTCCATATCGGCGGTATGACCGGCTCCCTCCAATCCATCAATTC
TCCATAGAAAGTGACACGGATCTCTCTGAGACTGCAGAGTTGATTGAGGAGTATGAGGTTTTTGATCCTACCAGACCTCGACCAAAAATC
ATTCTTGTTATAGGTGGTCCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTGCAGAACGATATGGATTCCAATACATTTCTGTGGGA
GAATTATTAAGAAAGAAGATCCACAGTACCAGCAGCAATAGGAAATGGAGTCTTATTGCCAAGATAATTACAACTGGAGAATTGGCCCCA
CAGGAAACAACAATTACAGAGATAAAGCAAAAATTGATGCAAATACCTGATGAAGAGGGCATTGTTATTGATGGATTTCCAAGAGATGTT
GCCCAGGCTCTATCTTTTGAGGACCAAATCTGTACCCCCGATTTGGTGGTATTCCTGGCTTGTGCTAATCAGAGACTCAAAGAAAGATTA
CTGAAGCGTGCAGAACAGCAGGGCCGACCAGACGACAATGTAAAAGCTACCCAAAGGAGACTAATGAACTTCAAGCAGAATGCTGCTCCA
TTGGTTAAATACTTCCAGGAAAAGGGGCTCATCATGACATTTGATGCCGACCGCGATGAGGATGAGGTGTTCTATGACATCAGCATGGCA
GTTGACAACAAGTTATTTCCAAACAAAGAGGCTGCAGCAGGTTCAAGTGACCTTGATCCTTCGATGATATTGGACACTGGAGAGATCATT
GATACAGGATCTGATTATGAAGATCAGGGTGATGACCAGTTAAATGTATTTGGAGAGGACACTATGGGAGGTTTCATGGAAGATTTGAGA
AAGTGTAAAATTATTTTCATAATTGGTGGTCCTGGCTCTGGCAAAGGCACACAGTGTGAAAAGCTGGTGGAAAAATATGGATTTACACAT
CTCTCAACTGGCGAGCTCCTGCGTGAGGAACTGGCATCAGAATCTGAAAGAAGCAAATTGATCAGAGACATTATGGAACGTGGAGACCTG
GTGCCCTCAGGCATCGTTTTGGAGCTCCTGAAGGAGGCCATGGTGGCCAGCCTCGGGGACACCAGGGGCTTCCTGATTGACGGCTATCCT
CGGGAGGTGAAGCAAGGGGAAGAGTTCGGACGCAGGATTGGAGACCCACAGTTGGTGATCTGTATGGACTGCTCGGCAGACACCATGACC
AACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGACCATCGCCAAGCGCCTAGAAGCCTACTACCGAGCG
TCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTT
TATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAG
ATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCT
ATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAAAAATTGAAGTA
GAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTGACATGTACACATGCGAAAACATT
CCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTCTTCTTAGTGGGCATTATGGAAGAATATATTAAAAT
GTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTTTCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTT
TGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCTC
GAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTAG
TCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGT
AATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATCCATAGTATCCTTTGTAGT
GTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTA
ATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCA
GGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTCTTTTTGAATGTATCAAGG
ATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAAACTTCAATTCCTGTAACC
TGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTTTTATTTTGAGAGCATGAAGTGTGGAATGTGTCACT
ACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGATAAAGTCTTTTTGAATAATATAGTATGCACTGCTA
TTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGATGTTGAATAGCATTCATG

>3250_3250_2_AK5-USP33_AK5_chr1_78001723_ENST00000344720_USP33_chr1_78167156_ENST00000370793_length(amino acids)=627AA_BP=518
MNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKWDTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDL
SETAELIEEYEVFDPTRPRPKIILVIGGPGSGKGTQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEI
KQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEK
GLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFII
GGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEE
FGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWF

--------------------------------------------------------------
>3250_3250_3_AK5-USP33_AK5_chr1_78001723_ENST00000344720_USP33_chr1_78167156_ENST00000370794_length(transcript)=4228nt_BP=2568nt
AGTCTTCCAGCAGGGTTCTGAAAGCAGCCTGCTCCCATCGCGCCCCTCGGATGTGGACACCCAGGGTGGGCTGCAGACCGCGGCCGGGCC
GCACTCTCTGGGGGCGAGGGCCAGGTCAGGTGGCTGCACTGTCGCCCAGCAGCGAGTAAAGACTCGAAACTGAGGGAGCCAGAGCCCTAG
TCACTCTGCTGGTCGAGGGTCGGAACCCCTGGGGGAAAGAGGAAATGTGGACGAAGGCAGCCCTTCCTGGAGACAGCTTGGAAGAGAGGG
ATGAAGATGCAAAAAGCAAAACCCAACCTATCAAGTGCCACCTCCCCGGTGACAGGCCCCCAGCCTTGCTCTGCGCTTTCGCTGAATGAA
CTCCTGAGCACGTTTTTCCCGTTGCCTAGGAAGGGTTGGGCCGCAGGTTTCGCCAGTGCTCGACAACCCACCTGGAGATAGCGGGTCGGG
GAGGGAGCTGCGAGGGGGGCGGACTCCTGTTGCTCAGTCTTCAGACCGCAGCACAGCATCATCTGCACCGGGACCCGGGAATGGGGGGAC
ACTTGGAGACTGAGAGAGAGGAGGCTCGAGGAATGAACCTTGGTCTGTTCTAAGCCACAACCCCTGTCTCAGGTCGTTTGGCTTCCTTTC
CTTCTCTTTGTATTTGAATTTAAAAACATTCCTGAATCTCAAGTGCTTTTGCTTGAAGTAGGGGGGAGGAGGAGAAAAGAATAGAAGCAA
GCCAGGCTTCCTAAGCGAGCTCTGAAAGGAGAAATCCATTCACTGCAAACCTCGTTCCCCATTTCTGAAAACGGATAGACTGATATCGTA
AACTAAAGGTCATGAGGGTTTTTCCCCCCGGTTTGGGGGATTTGTTCTGTTAAAAGGAATTTTAAAGCACCACTTGAGAAAATAATTTTG
CCTCAGTTTCCCTAAACTATCTTTAAAATGGGTTGCCATAATTACAGGTCTTCTTTTCTCTCACCTCTATAAGCCACCGTTTTCAGGTCA
TCTGAAGTCAAGATTTCCAGCCTTTTGAATGGACTGATGTGTTCTAAGCCCGAAGATCCAGTAGAATACTTGGAAAGTTGTTTACAAAAA
GTAAAGGAACTGGGTGGCTGTGACAAGGTGAAATGGGATACATTTGTAAGCCAGGAAAAGAAGACCTTACCTCCACTAAATGGAGGACAG
TCACGGAGATCCTTTCTAAGAAATGTAATGCCTGAAAACTCAAACTTTCCATATCGGCGGTATGACCGGCTCCCTCCAATCCATCAATTC
TCCATAGAAAGTGACACGGATCTCTCTGAGACTGCAGAGTTGATTGAGGAGTATGAGGTTTTTGATCCTACCAGACCTCGACCAAAAATC
ATTCTTGTTATAGGTGGTCCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTGCAGAACGATATGGATTCCAATACATTTCTGTGGGA
GAATTATTAAGAAAGAAGATCCACAGTACCAGCAGCAATAGGAAATGGAGTCTTATTGCCAAGATAATTACAACTGGAGAATTGGCCCCA
CAGGAAACAACAATTACAGAGATAAAGCAAAAATTGATGCAAATACCTGATGAAGAGGGCATTGTTATTGATGGATTTCCAAGAGATGTT
GCCCAGGCTCTATCTTTTGAGGACCAAATCTGTACCCCCGATTTGGTGGTATTCCTGGCTTGTGCTAATCAGAGACTCAAAGAAAGATTA
CTGAAGCGTGCAGAACAGCAGGGCCGACCAGACGACAATGTAAAAGCTACCCAAAGGAGACTAATGAACTTCAAGCAGAATGCTGCTCCA
TTGGTTAAATACTTCCAGGAAAAGGGGCTCATCATGACATTTGATGCCGACCGCGATGAGGATGAGGTGTTCTATGACATCAGCATGGCA
GTTGACAACAAGTTATTTCCAAACAAAGAGGCTGCAGCAGGTTCAAGTGACCTTGATCCTTCGATGATATTGGACACTGGAGAGATCATT
GATACAGGATCTGATTATGAAGATCAGGGTGATGACCAGTTAAATGTATTTGGAGAGGACACTATGGGAGGTTTCATGGAAGATTTGAGA
AAGTGTAAAATTATTTTCATAATTGGTGGTCCTGGCTCTGGCAAAGGCACACAGTGTGAAAAGCTGGTGGAAAAATATGGATTTACACAT
CTCTCAACTGGCGAGCTCCTGCGTGAGGAACTGGCATCAGAATCTGAAAGAAGCAAATTGATCAGAGACATTATGGAACGTGGAGACCTG
GTGCCCTCAGGCATCGTTTTGGAGCTCCTGAAGGAGGCCATGGTGGCCAGCCTCGGGGACACCAGGGGCTTCCTGATTGACGGCTATCCT
CGGGAGGTGAAGCAAGGGGAAGAGTTCGGACGCAGGATTGGAGACCCACAGTTGGTGATCTGTATGGACTGCTCGGCAGACACCATGACC
AACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGACCATCGCCAAGCGCCTAGAAGCCTACTACCGAGCG
TCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTT
TATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAG
ATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCT
ATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAAAAATTGAAGTA
GAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTGACATGTACACATGCGAAAACATT
CCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTCTTCTTAGTGGGCATTATGGAAGAATATATTAAAAT
GTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTTTCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTT
TGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCTC
GAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTAG
TCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGT
AATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATCCATAGTATCCTTTGTAGT
GTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTA
ATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCA
GGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTCTTTTTGAATGTATCAAGG
ATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAAACTTCAATTCCTGTAACC
TGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTTTTATTTTGAGAGCATGAAGTGTGGAATGTGTCACT
ACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGATAAAGTCTTTTTGAATAATATAGTATGCACTGCTA
TTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGATGTTGAATAGCATTCATG

>3250_3250_3_AK5-USP33_AK5_chr1_78001723_ENST00000344720_USP33_chr1_78167156_ENST00000370794_length(amino acids)=627AA_BP=518
MNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKWDTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDL
SETAELIEEYEVFDPTRPRPKIILVIGGPGSGKGTQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEI
KQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEK
GLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFII
GGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEE
FGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWF

--------------------------------------------------------------
>3250_3250_4_AK5-USP33_AK5_chr1_78001723_ENST00000354567_USP33_chr1_78167156_ENST00000357428_length(transcript)=3539nt_BP=1883nt
GCGGCACAGCTGCTCGGCGCCTGCAGCTCCGGCTCGGGGGCTGGAACCGAAGCGGGGGCGGCGGGAGCGCGGAGACCACAGCCCCCGGGG
AGAGGCGGAGGGGGTCCCTGGCCTGGGCGGAGAGGCTGAGCTGAGTGCGCGTGAGAAAGAGGGCTGCACCGCTGCTCGGCGCGGACTCTG
CCAGCCCCAGCTTCAGCCCCGGCTCAGGTCGCCGCAGCCCGGGAGCCTCCCCGCTTGCGCCCCAAGGCACGCGCGGCACAGCCATGAACA
CCAACGATGCCAAGGAGTATCTGGCCCGGAGGGAAATCCCTCAGCTTTTTGAGAGCCTTTTGAATGGACTGATGTGTTCTAAGCCCGAAG
ATCCAGTAGAATACTTGGAAAGTTGTTTACAAAAAGTAAAGGAACTGGGTGGCTGTGACAAGGTGAAATGGGATACATTTGTAAGCCAGG
AAAAGAAGACCTTACCTCCACTAAATGGAGGACAGTCACGGAGATCCTTTCTAAGAAATGTAATGCCTGAAAACTCAAACTTTCCATATC
GGCGGTATGACCGGCTCCCTCCAATCCATCAATTCTCCATAGAAAGTGACACGGATCTCTCTGAGACTGCAGAGTTGATTGAGGAGTATG
AGGTTTTTGATCCTACCAGACCTCGACCAAAAATCATTCTTGTTATAGGTGGTCCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTG
CAGAACGATATGGATTCCAATACATTTCTGTGGGAGAATTATTAAGAAAGAAGATCCACAGTACCAGCAGCAATAGGAAATGGAGTCTTA
TTGCCAAGATAATTACAACTGGAGAATTGGCCCCACAGGAAACAACAATTACAGAGATAAAGCAAAAATTGATGCAAATACCTGATGAAG
AGGGCATTGTTATTGATGGATTTCCAAGAGATGTTGCCCAGGCTCTATCTTTTGAGGACCAAATCTGTACCCCCGATTTGGTGGTATTCC
TGGCTTGTGCTAATCAGAGACTCAAAGAAAGATTACTGAAGCGTGCAGAACAGCAGGGCCGACCAGACGACAATGTAAAAGCTACCCAAA
GGAGACTAATGAACTTCAAGCAGAATGCTGCTCCATTGGTTAAATACTTCCAGGAAAAGGGGCTCATCATGACATTTGATGCCGACCGCG
ATGAGGATGAGGTGTTCTATGACATCAGCATGGCAGTTGACAACAAGTTATTTCCAAACAAAGAGGCTGCAGCAGGTTCAAGTGACCTTG
ATCCTTCGATGATATTGGACACTGGAGAGATCATTGATACAGGATCTGATTATGAAGATCAGGGTGATGACCAGTTAAATGTATTTGGAG
AGGACACTATGGGAGGTTTCATGGAAGATTTGAGAAAGTGTAAAATTATTTTCATAATTGGTGGTCCTGGCTCTGGCAAAGGCACACAGT
GTGAAAAGCTGGTGGAAAAATATGGATTTACACATCTCTCAACTGGCGAGCTCCTGCGTGAGGAACTGGCATCAGAATCTGAAAGAAGCA
AATTGATCAGAGACATTATGGAACGTGGAGACCTGGTGCCCTCAGGCATCGTTTTGGAGCTCCTGAAGGAGGCCATGGTGGCCAGCCTCG
GGGACACCAGGGGCTTCCTGATTGACGGCTATCCTCGGGAGGTGAAGCAAGGGGAAGAGTTCGGACGCAGGATTGGAGACCCACAGTTGG
TGATCTGTATGGACTGCTCGGCAGACACCATGACCAACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGA
CCATCGCCAAGCGCCTAGAAGCCTACTACCGAGCGTCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACA
GAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAG
ATGGAGATCCTCCAGGTCCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCC
AGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATC
CAGATATACTTCAAGCAGAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTT
TCATGTGCCCTGACATGTACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTC
TTCTTAGTGGGCATTATGGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTT
TCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCT
CCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTA
GTAGAGATGGGGTTTCACCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGG
ATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTT
TACACTACATGCAGATCCATAGTATCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTC
TGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAG
TAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATT
AAAATTTACAAAATGTCTTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTT
ATATTGTACAATGTTAAACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTT
TTATTTTGAGAGCATGAAGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGA
TAAAGTCTTTTTGAATAATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATA
CTGGATTACATTGAGGATGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAA

>3250_3250_4_AK5-USP33_AK5_chr1_78001723_ENST00000354567_USP33_chr1_78167156_ENST00000357428_length(amino acids)=683AA_BP=574
MLGADSASPSFSPGSGRRSPGASPLAPQGTRGTAMNTNDAKEYLARREIPQLFESLLNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKW
DTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGGPGSGKG
TQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEIKQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICT
PDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAA
AGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELA
SESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLP
VDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLR

--------------------------------------------------------------
>3250_3250_5_AK5-USP33_AK5_chr1_78001723_ENST00000354567_USP33_chr1_78167156_ENST00000370793_length(transcript)=3539nt_BP=1883nt
GCGGCACAGCTGCTCGGCGCCTGCAGCTCCGGCTCGGGGGCTGGAACCGAAGCGGGGGCGGCGGGAGCGCGGAGACCACAGCCCCCGGGG
AGAGGCGGAGGGGGTCCCTGGCCTGGGCGGAGAGGCTGAGCTGAGTGCGCGTGAGAAAGAGGGCTGCACCGCTGCTCGGCGCGGACTCTG
CCAGCCCCAGCTTCAGCCCCGGCTCAGGTCGCCGCAGCCCGGGAGCCTCCCCGCTTGCGCCCCAAGGCACGCGCGGCACAGCCATGAACA
CCAACGATGCCAAGGAGTATCTGGCCCGGAGGGAAATCCCTCAGCTTTTTGAGAGCCTTTTGAATGGACTGATGTGTTCTAAGCCCGAAG
ATCCAGTAGAATACTTGGAAAGTTGTTTACAAAAAGTAAAGGAACTGGGTGGCTGTGACAAGGTGAAATGGGATACATTTGTAAGCCAGG
AAAAGAAGACCTTACCTCCACTAAATGGAGGACAGTCACGGAGATCCTTTCTAAGAAATGTAATGCCTGAAAACTCAAACTTTCCATATC
GGCGGTATGACCGGCTCCCTCCAATCCATCAATTCTCCATAGAAAGTGACACGGATCTCTCTGAGACTGCAGAGTTGATTGAGGAGTATG
AGGTTTTTGATCCTACCAGACCTCGACCAAAAATCATTCTTGTTATAGGTGGTCCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTG
CAGAACGATATGGATTCCAATACATTTCTGTGGGAGAATTATTAAGAAAGAAGATCCACAGTACCAGCAGCAATAGGAAATGGAGTCTTA
TTGCCAAGATAATTACAACTGGAGAATTGGCCCCACAGGAAACAACAATTACAGAGATAAAGCAAAAATTGATGCAAATACCTGATGAAG
AGGGCATTGTTATTGATGGATTTCCAAGAGATGTTGCCCAGGCTCTATCTTTTGAGGACCAAATCTGTACCCCCGATTTGGTGGTATTCC
TGGCTTGTGCTAATCAGAGACTCAAAGAAAGATTACTGAAGCGTGCAGAACAGCAGGGCCGACCAGACGACAATGTAAAAGCTACCCAAA
GGAGACTAATGAACTTCAAGCAGAATGCTGCTCCATTGGTTAAATACTTCCAGGAAAAGGGGCTCATCATGACATTTGATGCCGACCGCG
ATGAGGATGAGGTGTTCTATGACATCAGCATGGCAGTTGACAACAAGTTATTTCCAAACAAAGAGGCTGCAGCAGGTTCAAGTGACCTTG
ATCCTTCGATGATATTGGACACTGGAGAGATCATTGATACAGGATCTGATTATGAAGATCAGGGTGATGACCAGTTAAATGTATTTGGAG
AGGACACTATGGGAGGTTTCATGGAAGATTTGAGAAAGTGTAAAATTATTTTCATAATTGGTGGTCCTGGCTCTGGCAAAGGCACACAGT
GTGAAAAGCTGGTGGAAAAATATGGATTTACACATCTCTCAACTGGCGAGCTCCTGCGTGAGGAACTGGCATCAGAATCTGAAAGAAGCA
AATTGATCAGAGACATTATGGAACGTGGAGACCTGGTGCCCTCAGGCATCGTTTTGGAGCTCCTGAAGGAGGCCATGGTGGCCAGCCTCG
GGGACACCAGGGGCTTCCTGATTGACGGCTATCCTCGGGAGGTGAAGCAAGGGGAAGAGTTCGGACGCAGGATTGGAGACCCACAGTTGG
TGATCTGTATGGACTGCTCGGCAGACACCATGACCAACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGA
CCATCGCCAAGCGCCTAGAAGCCTACTACCGAGCGTCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACA
GAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAG
ATGGAGATCCTCCAGGTCCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCC
AGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATC
CAGATATACTTCAAGCAGAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTT
TCATGTGCCCTGACATGTACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTC
TTCTTAGTGGGCATTATGGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTT
TCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCT
CCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTA
GTAGAGATGGGGTTTCACCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGG
ATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTT
TACACTACATGCAGATCCATAGTATCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTC
TGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAG
TAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATT
AAAATTTACAAAATGTCTTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTT
ATATTGTACAATGTTAAACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTT
TTATTTTGAGAGCATGAAGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGA
TAAAGTCTTTTTGAATAATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATA
CTGGATTACATTGAGGATGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAA

>3250_3250_5_AK5-USP33_AK5_chr1_78001723_ENST00000354567_USP33_chr1_78167156_ENST00000370793_length(amino acids)=683AA_BP=574
MLGADSASPSFSPGSGRRSPGASPLAPQGTRGTAMNTNDAKEYLARREIPQLFESLLNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKW
DTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGGPGSGKG
TQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEIKQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICT
PDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAA
AGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELA
SESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLP
VDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLR

--------------------------------------------------------------
>3250_3250_6_AK5-USP33_AK5_chr1_78001723_ENST00000354567_USP33_chr1_78167156_ENST00000370794_length(transcript)=3543nt_BP=1883nt
GCGGCACAGCTGCTCGGCGCCTGCAGCTCCGGCTCGGGGGCTGGAACCGAAGCGGGGGCGGCGGGAGCGCGGAGACCACAGCCCCCGGGG
AGAGGCGGAGGGGGTCCCTGGCCTGGGCGGAGAGGCTGAGCTGAGTGCGCGTGAGAAAGAGGGCTGCACCGCTGCTCGGCGCGGACTCTG
CCAGCCCCAGCTTCAGCCCCGGCTCAGGTCGCCGCAGCCCGGGAGCCTCCCCGCTTGCGCCCCAAGGCACGCGCGGCACAGCCATGAACA
CCAACGATGCCAAGGAGTATCTGGCCCGGAGGGAAATCCCTCAGCTTTTTGAGAGCCTTTTGAATGGACTGATGTGTTCTAAGCCCGAAG
ATCCAGTAGAATACTTGGAAAGTTGTTTACAAAAAGTAAAGGAACTGGGTGGCTGTGACAAGGTGAAATGGGATACATTTGTAAGCCAGG
AAAAGAAGACCTTACCTCCACTAAATGGAGGACAGTCACGGAGATCCTTTCTAAGAAATGTAATGCCTGAAAACTCAAACTTTCCATATC
GGCGGTATGACCGGCTCCCTCCAATCCATCAATTCTCCATAGAAAGTGACACGGATCTCTCTGAGACTGCAGAGTTGATTGAGGAGTATG
AGGTTTTTGATCCTACCAGACCTCGACCAAAAATCATTCTTGTTATAGGTGGTCCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTG
CAGAACGATATGGATTCCAATACATTTCTGTGGGAGAATTATTAAGAAAGAAGATCCACAGTACCAGCAGCAATAGGAAATGGAGTCTTA
TTGCCAAGATAATTACAACTGGAGAATTGGCCCCACAGGAAACAACAATTACAGAGATAAAGCAAAAATTGATGCAAATACCTGATGAAG
AGGGCATTGTTATTGATGGATTTCCAAGAGATGTTGCCCAGGCTCTATCTTTTGAGGACCAAATCTGTACCCCCGATTTGGTGGTATTCC
TGGCTTGTGCTAATCAGAGACTCAAAGAAAGATTACTGAAGCGTGCAGAACAGCAGGGCCGACCAGACGACAATGTAAAAGCTACCCAAA
GGAGACTAATGAACTTCAAGCAGAATGCTGCTCCATTGGTTAAATACTTCCAGGAAAAGGGGCTCATCATGACATTTGATGCCGACCGCG
ATGAGGATGAGGTGTTCTATGACATCAGCATGGCAGTTGACAACAAGTTATTTCCAAACAAAGAGGCTGCAGCAGGTTCAAGTGACCTTG
ATCCTTCGATGATATTGGACACTGGAGAGATCATTGATACAGGATCTGATTATGAAGATCAGGGTGATGACCAGTTAAATGTATTTGGAG
AGGACACTATGGGAGGTTTCATGGAAGATTTGAGAAAGTGTAAAATTATTTTCATAATTGGTGGTCCTGGCTCTGGCAAAGGCACACAGT
GTGAAAAGCTGGTGGAAAAATATGGATTTACACATCTCTCAACTGGCGAGCTCCTGCGTGAGGAACTGGCATCAGAATCTGAAAGAAGCA
AATTGATCAGAGACATTATGGAACGTGGAGACCTGGTGCCCTCAGGCATCGTTTTGGAGCTCCTGAAGGAGGCCATGGTGGCCAGCCTCG
GGGACACCAGGGGCTTCCTGATTGACGGCTATCCTCGGGAGGTGAAGCAAGGGGAAGAGTTCGGACGCAGGATTGGAGACCCACAGTTGG
TGATCTGTATGGACTGCTCGGCAGACACCATGACCAACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGA
CCATCGCCAAGCGCCTAGAAGCCTACTACCGAGCGTCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACA
GAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAG
ATGGAGATCCTCCAGGTCCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCC
AGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATC
CAGATATACTTCAAGCAGAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTT
TCATGTGCCCTGACATGTACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTC
TTCTTAGTGGGCATTATGGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTT
TCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCT
CCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTA
GTAGAGATGGGGTTTCACCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGG
ATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTT
TACACTACATGCAGATCCATAGTATCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTC
TGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAG
TAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATT
AAAATTTACAAAATGTCTTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTT
ATATTGTACAATGTTAAACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTT
TTATTTTGAGAGCATGAAGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGA
TAAAGTCTTTTTGAATAATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATA
CTGGATTACATTGAGGATGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAA

>3250_3250_6_AK5-USP33_AK5_chr1_78001723_ENST00000354567_USP33_chr1_78167156_ENST00000370794_length(amino acids)=683AA_BP=574
MLGADSASPSFSPGSGRRSPGASPLAPQGTRGTAMNTNDAKEYLARREIPQLFESLLNGLMCSKPEDPVEYLESCLQKVKELGGCDKVKW
DTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGGPGSGKG
TQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEIKQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICT
PDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAA
AGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELA
SESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLP
VDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLR

--------------------------------------------------------------
>3250_3250_7_AK5-USP33_AK5_chr1_78001723_ENST00000478255_USP33_chr1_78167156_ENST00000357428_length(transcript)=2082nt_BP=426nt
CTCCACACCTCCCTGCAAGCTAAGGAAGCCGGCTCCGGCCTTGGGCAGCCCAGAAAGGGGCTCCCACAGTGCAGCGGCGGGCTGAAGGGC
TCCTCAAGCGCGGCCAGAGTGGGCGCCAAGGCGGAGGAGGCGCTGACATCGAGCGGGGGCTGTGAGGGCTGCCAGCATGCTGTCACCTCT
CAGTCCCACTAGATCCCAAGAGGCAGCAGAGGCAGCTTCTGAAAAATAAAATAAATTGGAGACCCACAGTTGGTGATCTGTATGGACTGC
TCGGCAGACACCATGACCAACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGACCATCGCCAAGCGCCTA
GAAGCCTACTACCGAGCGTCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAG
GACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGT
CCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACA
TGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCA
GAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTGACATG
TACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTCTTCTTAGTGGGCATTAT
GGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTTTCAGTGTAATTTTTTTT
TGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATT
CTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAGATGGGGTTTCA
CCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA
CAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATC
CATAGTATCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTT
CTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTG
TTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTC
TTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAA
ACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTTTTATTTTGAGAGCATGA
AGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGATAAAGTCTTTTTGAATA
ATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGA
TGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAATTAGCTCAATAAAAATG

>3250_3250_7_AK5-USP33_AK5_chr1_78001723_ENST00000478255_USP33_chr1_78167156_ENST00000357428_length(amino acids)=168AA_BP=59
MVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWFREWESFVKG

--------------------------------------------------------------
>3250_3250_8_AK5-USP33_AK5_chr1_78001723_ENST00000478255_USP33_chr1_78167156_ENST00000370793_length(transcript)=2082nt_BP=426nt
CTCCACACCTCCCTGCAAGCTAAGGAAGCCGGCTCCGGCCTTGGGCAGCCCAGAAAGGGGCTCCCACAGTGCAGCGGCGGGCTGAAGGGC
TCCTCAAGCGCGGCCAGAGTGGGCGCCAAGGCGGAGGAGGCGCTGACATCGAGCGGGGGCTGTGAGGGCTGCCAGCATGCTGTCACCTCT
CAGTCCCACTAGATCCCAAGAGGCAGCAGAGGCAGCTTCTGAAAAATAAAATAAATTGGAGACCCACAGTTGGTGATCTGTATGGACTGC
TCGGCAGACACCATGACCAACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGACCATCGCCAAGCGCCTA
GAAGCCTACTACCGAGCGTCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAG
GACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGT
CCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACA
TGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCA
GAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTGACATG
TACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTCTTCTTAGTGGGCATTAT
GGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTTTCAGTGTAATTTTTTTT
TGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATT
CTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAGATGGGGTTTCA
CCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA
CAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATC
CATAGTATCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTT
CTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTG
TTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTC
TTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAA
ACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTTTTATTTTGAGAGCATGA
AGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGATAAAGTCTTTTTGAATA
ATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGA
TGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAATTAGCTCAATAAAAATG

>3250_3250_8_AK5-USP33_AK5_chr1_78001723_ENST00000478255_USP33_chr1_78167156_ENST00000370793_length(amino acids)=168AA_BP=59
MVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWFREWESFVKG

--------------------------------------------------------------
>3250_3250_9_AK5-USP33_AK5_chr1_78001723_ENST00000478255_USP33_chr1_78167156_ENST00000370794_length(transcript)=2086nt_BP=426nt
CTCCACACCTCCCTGCAAGCTAAGGAAGCCGGCTCCGGCCTTGGGCAGCCCAGAAAGGGGCTCCCACAGTGCAGCGGCGGGCTGAAGGGC
TCCTCAAGCGCGGCCAGAGTGGGCGCCAAGGCGGAGGAGGCGCTGACATCGAGCGGGGGCTGTGAGGGCTGCCAGCATGCTGTCACCTCT
CAGTCCCACTAGATCCCAAGAGGCAGCAGAGGCAGCTTCTGAAAAATAAAATAAATTGGAGACCCACAGTTGGTGATCTGTATGGACTGC
TCGGCAGACACCATGACCAACCGCCTTCTCCAAAGGAGCCGGAGCAGCCTGCCTGTGGACGACACCACCAAGACCATCGCCAAGCGCCTA
GAAGCCTACTACCGAGCGTCCATCCCCGTGATCGCCTACTACGAGACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAG
GACTCTCCAGCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGT
CCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACA
TGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCA
GAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTGACATG
TACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTATTTCTTCTTAGTGGGCATTAT
GGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTTTCAGTGTAATTTTTTTT
TGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATT
CTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAGATGGGGTTTCA
CCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA
CAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATC
CATAGTATCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTT
CTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTG
TTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTC
TTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAA
ACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCACTTTTTATTTTGAGAGCATGA
AGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGATAAAGTCTTTTTGAATA
ATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGA
TGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAATTAGCTCAATAAAAATG

>3250_3250_9_AK5-USP33_AK5_chr1_78001723_ENST00000478255_USP33_chr1_78167156_ENST00000370794_length(amino acids)=168AA_BP=59
MVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKLNRAFQKEDSPATFYCISMQWFREWESFVKG

--------------------------------------------------------------

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Fusion Gene PPI Analysis for AK5-USP33


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for AK5-USP33


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for AK5-USP33


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource