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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:HCN1-SPEF2 (FusionGDB2 ID:35780) |
Fusion Gene Summary for HCN1-SPEF2 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: HCN1-SPEF2 | Fusion gene ID: 35780 | Hgene | Tgene | Gene symbol | HCN1 | SPEF2 | Gene ID | 348980 | 79925 |
| Gene name | hyperpolarization activated cyclic nucleotide gated potassium channel 1 | sperm flagellar 2 | |
| Synonyms | BCNG-1|BCNG1|EIEE24|GEFSP10|HAC-2 | CT122|KPL2|SPGF43 | |
| Cytomap | 5p12 | 5p13.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1brain cyclic nucleotide-gated channel 1 | sperm flagellar protein 2cancer/testis antigen 122testis tissue sperm-binding protein Li 47a | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | O60741 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000303230, | ENST00000303129, ENST00000282469, ENST00000356031, ENST00000440995, ENST00000509059, | |
| Fusion gene scores | * DoF score | 2 X 2 X 2=8 | 8 X 10 X 12=960 |
| # samples | 2 | 16 | |
| ** MAII score | log2(2/8*10)=1.32192809488736 | log2(16/960*10)=-2.58496250072116 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: HCN1 [Title/Abstract] AND SPEF2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | HCN1(45645287)-SPEF2(35667174), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | HCN1 | GO:0051289 | protein homotetramerization | 28086084 |
| Hgene | HCN1 | GO:0071320 | cellular response to cAMP | 22748890 |
| Fusion gene breakpoints across HCN1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across SPEF2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | SKCM | TCGA-D3-A1Q3-06A | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + |
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Fusion Gene ORF analysis for HCN1-SPEF2 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-intron | ENST00000303230 | ENST00000303129 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + |
| In-frame | ENST00000303230 | ENST00000282469 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + |
| In-frame | ENST00000303230 | ENST00000356031 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + |
| In-frame | ENST00000303230 | ENST00000440995 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + |
| In-frame | ENST00000303230 | ENST00000509059 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000303230 | HCN1 | chr5 | 45645287 | - | ENST00000282469 | SPEF2 | chr5 | 35667174 | + | 2612 | 907 | 58 | 1284 | 408 |
| ENST00000303230 | HCN1 | chr5 | 45645287 | - | ENST00000509059 | SPEF2 | chr5 | 35667174 | + | 4293 | 907 | 58 | 2571 | 837 |
| ENST00000303230 | HCN1 | chr5 | 45645287 | - | ENST00000356031 | SPEF2 | chr5 | 35667174 | + | 5267 | 907 | 58 | 5208 | 1716 |
| ENST00000303230 | HCN1 | chr5 | 45645287 | - | ENST00000440995 | SPEF2 | chr5 | 35667174 | + | 5704 | 907 | 58 | 5196 | 1712 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000303230 | ENST00000282469 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + | 0.000223872 | 0.9997762 |
| ENST00000303230 | ENST00000509059 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + | 0.000708108 | 0.9992919 |
| ENST00000303230 | ENST00000356031 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + | 0.000477783 | 0.9995222 |
| ENST00000303230 | ENST00000440995 | HCN1 | chr5 | 45645287 | - | SPEF2 | chr5 | 35667174 | + | 0.000554314 | 0.9994456 |
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Fusion Genomic Features for HCN1-SPEF2 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| HCN1 | chr5 | 45645286 | - | SPEF2 | chr5 | 35667173 | + | 0.000944756 | 0.9990552 |
| HCN1 | chr5 | 45645286 | - | SPEF2 | chr5 | 35667173 | + | 0.000944756 | 0.9990552 |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
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| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for HCN1-SPEF2 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:45645287/chr5:35667174) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| HCN1 | . |
| FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions (PubMed:28086084). Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli. {ECO:0000269|PubMed:15351778, ECO:0000269|PubMed:28086084, ECO:0000269|PubMed:29936235, ECO:0000269|PubMed:30351409}. | FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 1_80 | 283 | 891.0 | Compositional bias | Note=Gly-rich |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 1_93 | 283 | 891.0 | Region | Intrinsically disordered |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 165_173 | 283 | 891.0 | Topological domain | Extracellular |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 195_215 | 283 | 891.0 | Topological domain | Cytoplasmic |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 1_142 | 283 | 891.0 | Topological domain | Cytoplasmic |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 237_260 | 283 | 891.0 | Topological domain | Extracellular |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 143_164 | 283 | 891.0 | Transmembrane | Helical%3B Name%3DSegment S1 |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 174_194 | 283 | 891.0 | Transmembrane | Helical%3B Name%3DSegment S2 |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 216_236 | 283 | 891.0 | Transmembrane | Helical%3B Name%3DSegment S3 |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 261_281 | 283 | 891.0 | Transmembrane | Helical%3B Voltage-sensor%3B Name%3DSegment S4 |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000282469 | 7 | 10 | 1686_1712 | 389 | 515.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000282469 | 7 | 10 | 732_758 | 389 | 515.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000282469 | 7 | 10 | 871_909 | 389 | 515.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000303129 | 0 | 13 | 1686_1712 | 0 | 620.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000303129 | 0 | 13 | 227_260 | 0 | 620.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000303129 | 0 | 13 | 321_396 | 0 | 620.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000303129 | 0 | 13 | 732_758 | 0 | 620.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000303129 | 0 | 13 | 871_909 | 0 | 620.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000356031 | 7 | 37 | 1686_1712 | 389 | 1823.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000356031 | 7 | 37 | 732_758 | 389 | 1823.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000356031 | 7 | 37 | 871_909 | 389 | 1823.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000440995 | 7 | 37 | 1686_1712 | 389 | 1819.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000440995 | 7 | 37 | 732_758 | 389 | 1819.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000440995 | 7 | 37 | 871_909 | 389 | 1819.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000303129 | 0 | 13 | 1_105 | 0 | 620.0 | Domain | Calponin-homology (CH) |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 726_756 | 283 | 891.0 | Compositional bias | Note=Gln-rich |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 857_863 | 283 | 891.0 | Compositional bias | Note=Poly-Pro |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 345_366 | 283 | 891.0 | Intramembrane | Pore-forming%3B Name%3DSegment H5 |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 358_362 | 283 | 891.0 | Motif | Selectivity filter |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 539_542 | 283 | 891.0 | Nucleotide binding | cAMP |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 549_550 | 283 | 891.0 | Nucleotide binding | cAMP |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 590_593 | 283 | 891.0 | Nucleotide binding | cAMP |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 282_295 | 283 | 891.0 | Topological domain | Cytoplasmic |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 319_344 | 283 | 891.0 | Topological domain | Extracellular |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 367_371 | 283 | 891.0 | Topological domain | Extracellular |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 393_890 | 283 | 891.0 | Topological domain | Cytoplasmic |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 296_318 | 283 | 891.0 | Transmembrane | Helical%3B Name%3DSegment S5 |
| Hgene | HCN1 | chr5:45645287 | chr5:35667174 | ENST00000303230 | - | 2 | 8 | 372_392 | 283 | 891.0 | Transmembrane | Helical%3B Name%3DSegment S6 |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000282469 | 7 | 10 | 227_260 | 389 | 515.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000282469 | 7 | 10 | 321_396 | 389 | 515.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000356031 | 7 | 37 | 227_260 | 389 | 1823.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000356031 | 7 | 37 | 321_396 | 389 | 1823.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000440995 | 7 | 37 | 227_260 | 389 | 1819.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000440995 | 7 | 37 | 321_396 | 389 | 1819.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000282469 | 7 | 10 | 1_105 | 389 | 515.0 | Domain | Calponin-homology (CH) | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000356031 | 7 | 37 | 1_105 | 389 | 1823.0 | Domain | Calponin-homology (CH) | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000440995 | 7 | 37 | 1_105 | 389 | 1819.0 | Domain | Calponin-homology (CH) |
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Fusion Gene Sequence for HCN1-SPEF2 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >35780_35780_1_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000282469_length(transcript)=2612nt_BP=907nt GGCGCCGGGCGGCCGGCGAGTCTGGAGCCCGCGCCGTCGCCGGCCGCGTCCTCCGGGCATGGAAGGAGGCGGCAAGCCCAACTCTTCGTC TAACAGCCGGGACGATGGCAACAGCGTCTTCCCCGCCAAGGCGTCCGCGACGGGCGCGGGGCCGGCCGCGGCCGAGAAGCGCCTGGGCAC CCCGCCGGGGGGCGGCGGGGCCGGCGCGAAGGAGCACGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGTGGCGGCGGTGGCGGCGGCGG CGGCGGCGGCGAGGAGCCGGCGGGGGGCTTCGAAGACGCCGAGGGGCCCCGGCGGCAGTACGGCTTCATGCAGAGGCAGTTCACCTCCAT GCTGCAGCCCGGGGTCAACAAATTCTCCCTCCGCATGTTTGGGAGCCAGAAGGCGGTGGAAAAGGAGCAGGAAAGGGTTAAAACTGCAGG CTTCTGGATTATCCACCCTTACAGTGATTTCAGGTTTTACTGGGATTTAATAATGCTTATAATGATGGTTGGAAATCTAGTCATCATACC AGTTGGAATCACATTCTTTACAGAGCAAACAACAACACCATGGATTATTTTCAATGTGGCATCAGATACAGTTTTCCTATTGGACCTGAT CATGAATTTTAGGACTGGGACTGTCAATGAAGACAGTTCTGAAATCATCCTGGACCCCAAAGTGATCAAGATGAATTATTTAAAAAGCTG GTTTGTGGTTGACTTCATCTCATCCATCCCAGTGGATTATATCTTTCTTATTGTAGAAAAAGGAATGGATTCTGAAGTTTACAAGACAGC CAGGGCACTTCGCATTGTGAGGTTTACAAAAATTCTCAGTCTCTTGCGTTTATTACGACTTTCAAGGTTAATTAGATACATACATCAATG GGAAGAGGCTTTGGCAAAACAAGCCAAGATTGACTTTGAAGAACAATTCCTTAAAGAAAAGAGATTTCATGATCAGATTGCTGTGGAAAG AGCTCAAGCTCGTTATGAAAAGCATTATTCAGTATGTGCAGAAATTTTGGATCAAATAGTTGATTTGTCCACTAAAGTGGCAGACTATCG AATGTTGACAAATAATCTGATTCCGTATAAGTTGATGCATGATTGGAAGGAACTATTTTTTAATGCAAAACCCATATATGAACAAGCCTC TGTTAAGACACTACCTGCTAACCCCTCAAGAGAACAACTTACAGAACTGGAGAAAAGGGACTTGCTAGATACCAATGATTATGAAGAATA TAAGGTACCTACTGATATGAAATAATTAGAATGCTACATAATAAATCCTTTTTCTTTAACATTAATTTTGTGATATTCCTTATTTCATTT CTACTAATAAAAATAGACATGTTTTGTTTGCATTTTCCCTTGTTTTAATTTGTATGTTTGTACTACTTTTTAGATGCTTAAACTATACAT AAGCTATTATCTATTGTTCCTAGTAATCTTAGGGGAGTGGTCTGAAAAAACATCAAGCATAGTAATTGTCTGGGTAGACAGATTTCAGAA TTTTTAAAATGCTTTAATTTATTTATTAGCATTTTTCTTTGTATGGGCTGTGTACTTTTGCTTCAAAAACCATTGTTAAAAAGTATTTCT TTTGTAATCAATCTAAATAATAGACTTTTTTTACAGGCAAAATGAAAGAGGAGGAAGTCTGGGTTTTATGAATAGCATAGATTCAAACAA TCTTTTATTCAAAGATTGAAATAAAAAGTATCGGGTGCCAACTGTGATTTTTAGAAGAACTGTTGTAAAGCCTTTGAGATTAGACAGACT AGAGCTACATTACTGAAATTGCCAACTACTAACTGTGTGACCTTAGGTGACCAGTTCATTAAGGGCTAATAGATTTCCATTTTTCAATTA ATGGAAAGGGAATGCTACTATACCTACTTCATTTAAAATAACATGAGTTAAACACTTAGCTGAGTGCACTTCATGTTGGATGCATATAAT ACATATCCATTTCTTTCCTCACCATCCTACTCTCCCCATCCCACTCCATCTCACCCTTAGAAGTAAAGGTGTAACAATCATAGCTTTTGG ATAGAAATTTTGAGTGGATCAACAGCAATGGAATGAGGAGTGAGGCTTTTTATTAGAAAAATTACAAAATGAGTCCCGGAAGGTCAGTAA GTGCAACATGAAAAAAATCTATGAAAAGATTATTTGACCTAATTATTCACTGATAGTACATACTCAACAAAGGTTTGTGGGACAAAGGGT GAATAAAATGAATGAATTACAATGAACAAAGAAAAGTGCAAAATAACACTAACAAGTAAATATTACAAGAAGATAACCTTGTGGTTAAAA GATAAGGAAAAATATGAGATTATGAGAAATTAAATGTATACTATATTTTAATTAAATGAATCTGTATTTGTAGCACTAAGAGAATTATTA TTAAGGATTGACTAGAACCTTAAAAGATATATGTGGATGTTTGTTTTGTACGGTTGTAAAATATACTAGACCTATTACATCCACCTAAAG TATAATGCCATGTAAGAAAATGCTTTGACAGTATCAACCTGTGTTAAGGTATTTCTTTCCATTGTAAATTAAAAGTCATTTTATTTGACA >35780_35780_1_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000282469_length(amino acids)=408AA_BP=283 MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQ YGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNV ASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLR LSRLIRYIHQWEEALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELF -------------------------------------------------------------- >35780_35780_2_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000356031_length(transcript)=5267nt_BP=907nt GGCGCCGGGCGGCCGGCGAGTCTGGAGCCCGCGCCGTCGCCGGCCGCGTCCTCCGGGCATGGAAGGAGGCGGCAAGCCCAACTCTTCGTC TAACAGCCGGGACGATGGCAACAGCGTCTTCCCCGCCAAGGCGTCCGCGACGGGCGCGGGGCCGGCCGCGGCCGAGAAGCGCCTGGGCAC CCCGCCGGGGGGCGGCGGGGCCGGCGCGAAGGAGCACGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGTGGCGGCGGTGGCGGCGGCGG CGGCGGCGGCGAGGAGCCGGCGGGGGGCTTCGAAGACGCCGAGGGGCCCCGGCGGCAGTACGGCTTCATGCAGAGGCAGTTCACCTCCAT GCTGCAGCCCGGGGTCAACAAATTCTCCCTCCGCATGTTTGGGAGCCAGAAGGCGGTGGAAAAGGAGCAGGAAAGGGTTAAAACTGCAGG CTTCTGGATTATCCACCCTTACAGTGATTTCAGGTTTTACTGGGATTTAATAATGCTTATAATGATGGTTGGAAATCTAGTCATCATACC AGTTGGAATCACATTCTTTACAGAGCAAACAACAACACCATGGATTATTTTCAATGTGGCATCAGATACAGTTTTCCTATTGGACCTGAT CATGAATTTTAGGACTGGGACTGTCAATGAAGACAGTTCTGAAATCATCCTGGACCCCAAAGTGATCAAGATGAATTATTTAAAAAGCTG GTTTGTGGTTGACTTCATCTCATCCATCCCAGTGGATTATATCTTTCTTATTGTAGAAAAAGGAATGGATTCTGAAGTTTACAAGACAGC CAGGGCACTTCGCATTGTGAGGTTTACAAAAATTCTCAGTCTCTTGCGTTTATTACGACTTTCAAGGTTAATTAGATACATACATCAATG GGAAGAGGCTTTGGCAAAACAAGCCAAGATTGACTTTGAAGAACAATTCCTTAAAGAAAAGAGATTTCATGATCAGATTGCTGTGGAAAG AGCTCAAGCTCGTTATGAAAAGCATTATTCAGTATGTGCAGAAATTTTGGATCAAATAGTTGATTTGTCCACTAAAGTGGCAGACTATCG AATGTTGACAAATAATCTGATTCCGTATAAGTTGATGCATGATTGGAAGGAACTATTTTTTAATGCAAAACCCATATATGAACAAGCCTC TGTTAAGACACTACCTGCTAACCCCTCAAGAGAACAACTTACAGAACTGGAGAAAAGGGACTTGCTAGATACCAATGATTATGAAGAATA TAAGAACATGGTTGGAGAGTGGGCCTTACCAGAAGAAATGGTTGACAATTTACCACCCTCCAACAATTGCATACTGGGCCATATTCTTCA CAGGCTAGCTGAAAAATCTCTTCCTCCTCGAGCGGAATCAACAACACCTGAATTACCTTCATTTGCTGTTAAAGGATGCTTATTGGGGAA AACATTAAGTGGAAAAACTACCATTTTAAGGTCTCTACAAAAAGACTTTCCTATACAGATACTTTCTATTGACACTCTTGTCCAAGAAGC TATCCAAGCATTTCATGACAATGAAAAAGTCAGTGAGGTTCTACCAATTCAGAAAAATGATGAAGAAGATGCTCTACCAGTTCTGCAAGA GGAGATTAAAGAAAGCCAGGATCCACAACATGTATTTTCAGCTGGTCCAGTTTCAGATGAAGTATTACCAGAAACAGAAGGTGAAACAAT GCTTAGTGCTAATGCTGATAAAACACCAAAAGCTGAAGAAGTCAAATCAAGTGATAGTTTCTTAAAACTCACTACACGTGCTCAGCTTGG TGCAAAATCAGAACAGTTGCTGAAGAAAGGAAAGAGCATTCCTGATGTGCTGCTTGTTGACATCATAGTAAATGCTATTAATGAGATACC TGTGAATCAAGACTGTATCCTAGATGGTTTTCCAATGACTTTAAACCAAGCACAGCTTCTGGAAGAAGCTCTTACAGGCTGCAATAGAAA CCTCACAGAAGTGGAAAGAAAAAAAGCACAAAAATCCACATTGGCTATTGATCCTGCGACTTCCAAAGAAATACCTCTTCCCTCTCCTGC ATTTGATTTTGTCATATTATTAGATGTTTCAGATACTTCCTCAATGAGTCGCATGAATGATATTATAGCTGAAGAATTGTCCTATAAAAC TGCTCACGAAGATATCAGTCAACGTGTAGCTGCTGAAAACCAAGATAAGGATGGAGACCAAAATTTAAGAGACCAGATACAACATAGAAT TATAGGCTTCTTGGACAACTGGCCTTTATTGGAGCAATGGTTTTCAGAGCCAGAAAATATTTTGATAAAAATCAATGCTGAAATAGATAA GGAATCTTTATGTGAAAAAGTAAAAGAAATTCTTACGACTGAAATAGCAAAAAAAAAGAATAAAGTTGAGAAGAAATTAGAAGAAAAGGA AGCTGAGAAAAAAGCAGCAGCTTCCCTGGCTGAGCTTCCACTTCCTACACCTCCTCCTGCTCCTCCTCCTGAACCAGAAAAAGAGAAGGA AATTCATCAAAGCCATGTGGCTTCAAAAACTCCTACTGCAAAAGGAAAACCTCAATCAGAAGCCCCGCATGGTAAGCAAGAATCTCTTCA GGAAGGAAAAGGGAAGAAAGGTGAGACCGCACTCAAAAGAAAAGGTTCTCCTAAAGGAAAATCATCAGGAGGAAAAGTACCAGTAAAGAA ATCACCTGCTGACTCTACAGATACATCACCTGTTGCAATAGTGCCACAGCCACCTAAGCCAGGATCAGAAGAATGGGTCTATGTGAATGA ACCAGTTCCTGAGGAAATGCCTTTGTTTTTAGTACCTTACTGGGAACTAATAGAAAATTCCTATATAAACACCATCAAAACAGTACTCAG GCATCTGAGGGAAGACCAGCATACTGTGCTTGCTTACTTATATGAGATTAGAACAAGTTTCCAGGAGTTTCTAAAGCGTCCGGATCACAA GCAAGATTTTGTAGCTCAATGGCAGGCTGATTTCAACTCCCTTCCTGATGACCTGTGGGATGATGAGGAAACAAAGGCTGAACTACATCA ACGAGTGAATGATCTGCGAGACCGCCTGTGGGACATTTGTGATGCCCGGAAGGAAGAGGCGGAGCAGGAGCGGCTTGACATCATTAATGA GAGCTGGTTACAGGACACTCTTGGAATGACAATGAACCATTTCTTTTCCCTGATGCAGGCAGAGCTGAACCGTTTCCAAGATACAAAGAG ACTCCTTCAAGATTATTACTGGGGAATGGAAAGTAAAATCCCAGTAGAGGACAACAAGAGATTTACTCGAATCCCTTTGGTCCAACTGGA TAGTAAAGACAATTCTGAAAGCCAGCTTAGAATTCCTCTAGTTCCTCGAATATCCATTTCTCTGGAAACAGTTACACCCAAACCAAAAAC AAAATCAGTACTGAAGGGCAAGATGGATAACTCCCTAGAAAACGTTGAGTCCAACTTTGAGGCCGATGAAAAGTTGGTCATGGACACCTG GCAGCAGGCTTCTTTAGCAGTATCTCACATGGTGGCTGCTGAAATTCATCAGAGGCTTATGGAAGAAGAAAAAGAAAACCAGCCAGCAGA CCCCAAAGAAAAATCTCCTCAGATGGGTGCAAATAAAAAAGTCAAAAAGGAGCCACCCAAGAAAAAACAGGAAGACAAAAAACCCAAAGG TAAATCACCACCTATGGCAGAAGCAACTCCTGTCATAGTAACAACAGAGGAAATTGCTGAAATCAAAAGGAAAAATGAACTGAGGGTCAA AATAAAAGAAGAACACCTTGCTGCCTTGCAATTTGAAGAAATAGCCACGCAATTTCGACTTGAACTGATAAAGACAAAAGCATTGGCTCT TCTTGAAGATTTAGTAACAAAGGTGGTTGATGTATATAAACTCATGGAAAAATGGCTTGGTGAGAGGTATTTGAATGAAATGGCCAGTAC AGAAAAATTAACTGACGTAGCTCGCTATCACATTGAAACATCTACAAAAATTCAGAATGAACTTTATTTAAGCCAAGAAGACTTCTTCAT TAATGGCAATATAAAAGTCTTCCCAGATCCTCCCCCATCAATACGTCCTCCACCTGTAGAAAAGGAAGAAGATGGTACCCTGACCATTGA ACAGCTTGACAGTCTTCGAGATCAGTTCTTAGATATGGCACCTAAAGGCATAATAGGAAATAAAGCATTTACTGACATTCTGATCGATTT GGTGACCCTGAACCTTGGCACAAACAACTTTCCTAGTAATTGGATGCACCTTACCCAACCTGAATTACAGGAATTAACATCTTTATTAAC AGTCAACTCCGAGTTCGTGGACTGGCGGAAGTTCCTGTTAGTAACCTCAATGCCTTGGCCCATTCCCTTGGAGGAGGAGCTCCTTGAGAC CCTTCAGAAGTTCAAGGCTGTGGATAAGGAGCAGTTGGGCACCATCACTTTTGAGCAGTATATGCAGGCTGGTCTGTGGTTTACAGGAGA TGAAGATATAAAAATTCCAGAAAATCCTCTTGAACCCCTTCCATTTAATAGGCAGGAGCATCTTATAGAGTTTTTCTTTAGGCTATTTGC TGACTATGAGAAGGATCCACCCCAGCTTGACTACACACAGATGCTGCTTTACTTTGCTTGCCACCCAGACACCGTGGAAGGAGTCTACAG GGCCCTCAGTGTGGCTGTTGGAACTCATGTCTTCCAACAAGTCAAAGCTTCCATTCCAAGTGCAGAAAAGACCTCCTCAACTGATGCAGG TCCAGCTGAGGAATTTCCTGAACCTGAGGAAAATGCTGCAAGAGAAGAAAGGAAATTAAAAGACGACACGGAGAAAAGGGAACAGAAGGA TGAAGAAATCCCTGAAAATGCAAACAATGAAAAGATGTCCATGGAAACACTACTCAAAGTGTTCAAAGGGGGAAGTGAAGCACAGGACTC CAATAGATTTGCCAGCCACCTAAAGATAGAGAACATTTATGCAGAGGGCTTCATAAAAACATTTCAAGACCTAGGTGCCAAGAACCTGGA GCCAATTGAAGTCGCTGTTCTCTTGAAGCATCCTTTTATTCAAGACCTGATTTCAAATTATTCAGACTATAAGTTTCCTGATATTAAAAT AATTCTCCAAAGGAGTGAACATGTACAAGGAAGTGATGGAGAGAGATCACCTTCAAGACATACAGAGGAAAAGAAATGAAGACAAAAGAG >35780_35780_2_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000356031_length(amino acids)=1716AA_BP=283 MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQ YGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNV ASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLR LSRLIRYIHQWEEALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELF FNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELP SFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSD EVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQL LEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGD QNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPP APPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPK PGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLW DDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNK RFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRL MEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFR LELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPV EKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPW PIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFA CHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLK VFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSR -------------------------------------------------------------- >35780_35780_3_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000440995_length(transcript)=5704nt_BP=907nt GGCGCCGGGCGGCCGGCGAGTCTGGAGCCCGCGCCGTCGCCGGCCGCGTCCTCCGGGCATGGAAGGAGGCGGCAAGCCCAACTCTTCGTC TAACAGCCGGGACGATGGCAACAGCGTCTTCCCCGCCAAGGCGTCCGCGACGGGCGCGGGGCCGGCCGCGGCCGAGAAGCGCCTGGGCAC CCCGCCGGGGGGCGGCGGGGCCGGCGCGAAGGAGCACGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGTGGCGGCGGTGGCGGCGGCGG CGGCGGCGGCGAGGAGCCGGCGGGGGGCTTCGAAGACGCCGAGGGGCCCCGGCGGCAGTACGGCTTCATGCAGAGGCAGTTCACCTCCAT GCTGCAGCCCGGGGTCAACAAATTCTCCCTCCGCATGTTTGGGAGCCAGAAGGCGGTGGAAAAGGAGCAGGAAAGGGTTAAAACTGCAGG CTTCTGGATTATCCACCCTTACAGTGATTTCAGGTTTTACTGGGATTTAATAATGCTTATAATGATGGTTGGAAATCTAGTCATCATACC AGTTGGAATCACATTCTTTACAGAGCAAACAACAACACCATGGATTATTTTCAATGTGGCATCAGATACAGTTTTCCTATTGGACCTGAT CATGAATTTTAGGACTGGGACTGTCAATGAAGACAGTTCTGAAATCATCCTGGACCCCAAAGTGATCAAGATGAATTATTTAAAAAGCTG GTTTGTGGTTGACTTCATCTCATCCATCCCAGTGGATTATATCTTTCTTATTGTAGAAAAAGGAATGGATTCTGAAGTTTACAAGACAGC CAGGGCACTTCGCATTGTGAGGTTTACAAAAATTCTCAGTCTCTTGCGTTTATTACGACTTTCAAGGTTAATTAGATACATACATCAATG GGAAGAGGCTTTGGCAAAACAAGCCAAGATTGACTTTGAAGAACAATTCCTTAAAGAAAAGAGATTTCATGATCAGATTGCTGTGGAAAG AGCTCAAGCTCGTTATGAAAAGCATTATTCAGTATGTGCAGAAATTTTGGATCAAATAGTTGATTTGTCCACTAAAGTGGCAGACTATCG AATGTTGACAAATAATCTGATTCCGTATAAGTTGATGCATGATTGGAAGGAACTATTTTTTAATGCAAAACCCATATATGAACAAGCCTC TGTTAAGACACTACCTGCTAACCCCTCAAGAGAACAACTTACAGAACTGGAGAAAAGGGACTTGCTAGATACCAATGATTATGAAGAATA TAAGAACATGGTTGGAGAGTGGGCCTTACCAGAAGAAATGGTTGACAATTTACCACCCTCCAACAATTGCATACTGGGCCATATTCTTCA CAGGCTAGCTGAAAAATCTCTTCCTCCTCGAGCGGAATCAACAACACCTGAATTACCTTCATTTGCTGTTAAAGGATGCTTATTGGGGAA AACATTAAGTGGAAAAACTACCATTTTAAGGTCTCTACAAAAAGACTTTCCTATACAGATACTTTCTATTGACACTCTTGTCCAAGAAGC TATCCAAGCATTTCATGACAATGAAAAAGTCAGTGAGGTTCTACCAATTCAGAAAAATGATGAAGAAGATGCTCTACCAGTTCTGCAAGA GGAGATTAAAGAAAGCCAGGATCCACAACATGTATTTTCAGCTGGTCCAGTTTCAGATGAAGTATTACCAGAAACAGAAGGTGCTAATGC TGATAAAACACCAAAAGCTGAAGAAGTCAAATCAAGTGATAGTTTCTTAAAACTCACTACACGTGCTCAGCTTGGTGCAAAATCAGAACA GTTGCTGAAGAAAGGAAAGAGCATTCCTGATGTGCTGCTTGTTGACATCATAGTAAATGCTATTAATGAGATACCTGTGAATCAAGACTG TATCCTAGATGGTTTTCCAATGACTTTAAACCAAGCACAGCTTCTGGAAGAAGCTCTTACAGGCTGCAATAGAAACCTCACAGAAGTGGA AAGAAAAAAAGCACAAAAATCCACATTGGCTATTGATCCTGCGACTTCCAAAGAAATACCTCTTCCCTCTCCTGCATTTGATTTTGTCAT ATTATTAGATGTTTCAGATACTTCCTCAATGAGTCGCATGAATGATATTATAGCTGAAGAATTGTCCTATAAAACTGCTCACGAAGATAT CAGTCAACGTGTAGCTGCTGAAAACCAAGATAAGGATGGAGACCAAAATTTAAGAGACCAGATACAACATAGAATTATAGGCTTCTTGGA CAACTGGCCTTTATTGGAGCAATGGTTTTCAGAGCCAGAAAATATTTTGATAAAAATCAATGCTGAAATAGATAAGGAATCTTTATGTGA AAAAGTAAAAGAAATTCTTACGACTGAAATAGCAAAAAAAAAGAATAAAGTTGAGAAGAAATTAGAAGAAAAGGAAGCTGAGAAAAAAGC AGCAGCTTCCCTGGCTGAGCTTCCACTTCCTACACCTCCTCCTGCTCCTCCTCCTGAACCAGAAAAAGAGAAGGAAATTCATCAAAGCCA TGTGGCTTCAAAAACTCCTACTGCAAAAGGAAAACCTCAATCAGAAGCCCCGCATGGTAAGCAAGAATCTCTTCAGGAAGGAAAAGGGAA GAAAGGTGAGACCGCACTCAAAAGAAAAGGTTCTCCTAAAGGAAAATCATCAGGAGGAAAAGTACCAGTAAAGAAATCACCTGCTGACTC TACAGATACATCACCTGTTGCAATAGTGCCACAGCCACCTAAGCCAGGATCAGAAGAATGGGTCTATGTGAATGAACCAGTTCCTGAGGA AATGCCTTTGTTTTTAGTACCTTACTGGGAACTAATAGAAAATTCCTATATAAACACCATCAAAACAGTACTCAGGCATCTGAGGGAAGA CCAGCATACTGTGCTTGCTTACTTATATGAGATTAGAACAAGTTTCCAGGAGTTTCTAAAGCGTCCGGATCACAAGCAAGATTTTGTAGC TCAATGGCAGGCTGATTTCAACTCCCTTCCTGATGACCTGTGGGATGATGAGGAAACAAAGGCTGAACTACATCAACGAGTGAATGATCT GCGAGACCGCCTGTGGGACATTTGTGATGCCCGGAAGGAAGAGGCGGAGCAGGAGCGGCTTGACATCATTAATGAGAGCTGGTTACAGGA CACTCTTGGAATGACAATGAACCATTTCTTTTCCCTGATGCAGGCAGAGCTGAACCGTTTCCAAGATACAAAGAGACTCCTTCAAGATTA TTACTGGGGAATGGAAAGTAAAATCCCAGTAGAGGACAACAAGAGATTTACTCGAATCCCTTTGGTCCAACTGGATAGTAAAGACAATTC TGAAAGCCAGCTTAGAATTCCTCTAGTTCCTCGAATATCCATTTCTCTGGAAACAGTTACACCCAAACCAAAAACAAAATCAGTACTGAA GGGCAAGATGGATAACTCCCTAGAAAACGTTGAGTCCAACTTTGAGGCCGATGAAAAGTTGGTCATGGACACCTGGCAGCAGGCTTCTTT AGCAGTATCTCACATGGTGGCTGCTGAAATTCATCAGAGGCTTATGGAAGAAGAAAAAGAAAACCAGCCAGCAGACCCCAAAGAAAAATC TCCTCAGATGGGTGCAAATAAAAAAGTCAAAAAGGAGCCACCCAAGAAAAAACAGGAAGACAAAAAACCCAAAGGTAAATCACCACCTAT GGCAGAAGCAACTCCTGTCATAGTAACAACAGAGGAAATTGCTGAAATCAAAAGGAAAAATGAACTGAGGGTCAAAATAAAAGAAGAACA CCTTGCTGCCTTGCAATTTGAAGAAATAGCCACGCAATTTCGACTTGAACTGATAAAGACAAAAGCATTGGCTCTTCTTGAAGATTTAGT AACAAAGGTGGTTGATGTATATAAACTCATGGAAAAATGGCTTGGTGAGAGGTATTTGAATGAAATGGCCAGTACAGAAAAATTAACTGA CGTAGCTCGCTATCACATTGAAACATCTACAAAAATTCAGAATGAACTTTATTTAAGCCAAGAAGACTTCTTCATTAATGGCAATATAAA AGTCTTCCCAGATCCTCCCCCATCAATACGTCCTCCACCTGTAGAAAAGGAAGAAGATGGTACCCTGACCATTGAACAGCTTGACAGTCT TCGAGATCAGTTCTTAGATATGGCACCTAAAGGCATAATAGGAAATAAAGCATTTACTGACATTCTGATCGATTTGGTGACCCTGAACCT TGGCACAAACAACTTTCCTAGTAATTGGATGCACCTTACCCAACCTGAATTACAGGAATTAACATCTTTATTAACAGTCAACTCCGAGTT CGTGGACTGGCGGAAGTTCCTGTTAGTAACCTCAATGCCTTGGCCCATTCCCTTGGAGGAGGAGCTCCTTGAGACCCTTCAGAAGTTCAA GGCTGTGGATAAGGAGCAGTTGGGCACCATCACTTTTGAGCAGTATATGCAGGCTGGTCTGTGGTTTACAGGAGATGAAGATATAAAAAT TCCAGAAAATCCTCTTGAACCCCTTCCATTTAATAGGCAGGAGCATCTTATAGAGTTTTTCTTTAGGCTATTTGCTGACTATGAGAAGGA TCCACCCCAGCTTGACTACACACAGATGCTGCTTTACTTTGCTTGCCACCCAGACACCGTGGAAGGAGTCTACAGGGCCCTCAGTGTGGC TGTTGGAACTCATGTCTTCCAACAAGTCAAAGCTTCCATTCCAAGTGCAGAAAAGACCTCCTCAACTGATGCAGGTCCAGCTGAGGAATT TCCTGAACCTGAGGAAAATGCTGCAAGAGAAGAAAGGAAATTAAAAGACGACACGGAGAAAAGGGAACAGAAGGATGAAGAAATCCCTGA AAATGCAAACAATGAAAAGATGTCCATGGAAACACTACTCAAAGTGTTCAAAGGGGGAAGTGAAGCACAGGACTCCAATAGATTTGCCAG CCACCTAAAGATAGAGAACATTTATGCAGAGGGCTTCATAAAAACATTTCAAGACCTAGGTGCCAAGAACCTGGAGCCAATTGAAGTCGC TGTTCTCTTGAAGCATCCTTTTATTCAAGACCTGATTTCAAATTATTCAGACTATAAGTTTCCTGTACACATCATCTATGTAGTGGAAAT GATAGTGTGTGCAAAAGGTTGGGCACCACTTCCATCAGGAAAACAGTGTGCAAGATGGAGAAACTAAGGACAAACCCCAGAGAAACTGGA CAAACCCAGCAGAAGAAAGGAAGAACTGCAACTCAGAAATCACATAACCACCAAGCAGGTCTGGACCACACATGCTAAATGTGCATCCAC TATGGCGAGTCCCAGGCCTTTAACAGCAGGGACTAAGGAGGGCAGAGGCTGACACCAACCCCCTTTCATACTGTAGCACTACATCCCTCT CAGATACCAGTTTAACACGAAGTCTCCCTGTTTGACTCCTGTGAGTTGTGTATTGAACTACAAAGTGTTTGCTGAGTTGAAAATAAAAGC GCTTTCACTTTGATAGAATGTAGATTGTGTATCTTGACTTCTCAATGATAGCAAGTTACATTTTGCAGTTTTCTTCCTATACCCACAAAT GAAACTATTCAGACTTAAATATCTTCCTGTGTGCCTAAACGTTTTTGTTCTAAGTTGCTGATCCTTTTAAATATTAAATTATTAATTAAT >35780_35780_3_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000440995_length(amino acids)=1712AA_BP=283 MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQ YGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNV ASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLR LSRLIRYIHQWEEALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELF FNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELP SFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSD EVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEAL TGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRD QIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPE PEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEE WVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEET KAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRI PLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEK ENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIK TKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEED GTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLE EELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDT VEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGG SEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARW -------------------------------------------------------------- >35780_35780_4_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000509059_length(transcript)=4293nt_BP=907nt GGCGCCGGGCGGCCGGCGAGTCTGGAGCCCGCGCCGTCGCCGGCCGCGTCCTCCGGGCATGGAAGGAGGCGGCAAGCCCAACTCTTCGTC TAACAGCCGGGACGATGGCAACAGCGTCTTCCCCGCCAAGGCGTCCGCGACGGGCGCGGGGCCGGCCGCGGCCGAGAAGCGCCTGGGCAC CCCGCCGGGGGGCGGCGGGGCCGGCGCGAAGGAGCACGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGTGGCGGCGGTGGCGGCGGCGG CGGCGGCGGCGAGGAGCCGGCGGGGGGCTTCGAAGACGCCGAGGGGCCCCGGCGGCAGTACGGCTTCATGCAGAGGCAGTTCACCTCCAT GCTGCAGCCCGGGGTCAACAAATTCTCCCTCCGCATGTTTGGGAGCCAGAAGGCGGTGGAAAAGGAGCAGGAAAGGGTTAAAACTGCAGG CTTCTGGATTATCCACCCTTACAGTGATTTCAGGTTTTACTGGGATTTAATAATGCTTATAATGATGGTTGGAAATCTAGTCATCATACC AGTTGGAATCACATTCTTTACAGAGCAAACAACAACACCATGGATTATTTTCAATGTGGCATCAGATACAGTTTTCCTATTGGACCTGAT CATGAATTTTAGGACTGGGACTGTCAATGAAGACAGTTCTGAAATCATCCTGGACCCCAAAGTGATCAAGATGAATTATTTAAAAAGCTG GTTTGTGGTTGACTTCATCTCATCCATCCCAGTGGATTATATCTTTCTTATTGTAGAAAAAGGAATGGATTCTGAAGTTTACAAGACAGC CAGGGCACTTCGCATTGTGAGGTTTACAAAAATTCTCAGTCTCTTGCGTTTATTACGACTTTCAAGGTTAATTAGATACATACATCAATG GGAAGAGGCTTTGGCAAAACAAGCCAAGATTGACTTTGAAGAACAATTCCTTAAAGAAAAGAGATTTCATGATCAGATTGCTGTGGAAAG AGCTCAAGCTCGTTATGAAAAGCATTATTCAGTATGTGCAGAAATTTTGGATCAAATAGTTGATTTGTCCACTAAAGTGGCAGACTATCG AATGTTGACAAATAATCTGATTCCGTATAAGTTGATGCATGATTGGAAGGAACTATTTTTTAATGCAAAACCCATATATGAACAAGCCTC TGTTAAGACACTACCTGCTAACCCCTCAAGAGAACAACTTACAGAACTGGAGAAAAGGGACTTGCTAGATACCAATGATTATGAAGAATA TAAGAACATGGTTGGAGAGTGGGCCTTACCAGAAGAAATGGTTGACAATTTACCACCCTCCAACAATTGCATACTGGGCCATATTCTTCA CAGGCTAGCTGAAAAATCTCTTCCTCCTCGAGCGGAATCAACAACACCTGAATTACCTTCATTTGCTGTTAAAGGATGCTTATTGGGGAA AACATTAAGTGGAAAAACTACCATTTTAAGGTCTCTACAAAAAGACTTTCCTATACAGATACTTTCTATTGACACTCTTGTCCAAGAAGC TATCCAAGCATTTCATGACAATGAAAAAGTCAGTGAGGTTCTACCAATTCAGAAAAATGATGAAGAAGATGCTCTACCAGTTCTGCAAGA GGAGATTAAAGAAAGCCAGGATCCACAACATGTATTTTCAGCTGGTCCAGTTTCAGATGAAGTATTACCAGAAACAGAAGGTGCTAATGC TGATAAAACACCAAAAGCTGAAGAAGTCAAATCAAGTGATAGTTTCTTAAAACTCACTACACGTGCTCAGCTTGGTGCAAAATCAGAACA GTTGCTGAAGAAAGGAAAGAGCATTCCTGATGTGCTGCTTGTTGACATCATAGTAAATGCTATTAATGAGATACCTGTGAATCAAGACTG TATCCTAGATGGTTTTCCAATGACTTTAAACCAAGCACAGCTTCTGGAAGAAGCTCTTACAGGCTGCAATAGAAACCTCACAGAAGTGGA AAGAAAAAAAGCACAAAAATCCACATTGGCTATTGATCCTGCGACTTCCAAAGAAATACCTCTTCCCTCTCCTGCATTTGATTTTGTCAT ATTATTAGATGTTTCAGATACTTCCTCAATGAGTCGCATGAATGATATTATAGCTGAAGAATTGTCCTATAAAACTGCTCACGAAGATAT CAGTCAACGTGTAGCTGCTGAAAACCAAGATAAGGATGGAGACCAAAATTTAAGAGACCAGATACAACATAGAATTATAGGCTTCTTGGA CAACTGGCCTTTATTGGAGCAATGGTTTTCAGAGCCAGAAAATATTTTGATAAAAATCAATGCTGAAATAGATAAGGAATCTTTATGTGA AAAAGTAAAAGAAATTCTTACGACTGAAATAGCAAAAAAAAAGAATAAAGTTGAGAAGAAATTAGAAGAAAAGGAAGCTGAGAAAAAAGC AGCAGCTTCCCTGGCTGAGCTTCCACTTCCTACACCTCCTCCTGCTCCTCCTCCTGAACCAGAAAAAGAGAAGGAAATTCATCAAAGCCA TGTGGCTTCAAAAACTCCTACTGCAAAAGGAAAACCTCAATCAGGTGATTGACAGAATGATTTATAATCCTGTTTTCAGTTTCTTATTTT TACTCAGTAAAGAATTATAAATTATAGTCTATCTACATTCAGACTTTGGGCAACTTCAATCATATGAAACAAGGCTTTACACTCAGATGG AAGTGGAAAAGCCCTCGGAGTAGCTAAAAGCGTAGTTGTTACAAAGCCCAACTTCTTTGCCATGTAGGAGGAGACATGGAGTCATCACAC CTAGGTCTACTGATTAGAGTAAGACCACAGACGAGAAGAGGAAAAGGCACAGAGTAACAGGTAACTTCAGGCAGATCAAGAGGATACACG TCTGTGTGCTGAACTATAGAGTTACATTTATGTAATGAGTAAGAACATCTTAATAAAATAAGAATGAGTATAGCTGGTTTTCCACCAGAA TAAGGGAACAAATTAACAGCAACCATTAGAACTAGAATTTATATGTAATTGTAAATCTTCAGGACTATGTGATTATAAAAGTATAGAAAC AAGCTCCTGCCATCTCAGTAAACATATATTTATGGACACAGAGATAAAGAGCCTAGATAGAGAACACTTTTAGTTTAAGTTTGTTCTAGA TCCTTAACCGCTATGGACCATAAGTCTAGCATTAAGTGGCAGACAATGAATATAATGACAAAAGTGAAGTGCTTAATTGTTGTAATGCAT GATATTGGTAGAGTGTCAGTATCCATTTGTGTGTGTATATAAAACAAATGAGGCTCTGGTTTCTAGTTTCAGCTTTATAAATGGCTGACT GTGCAGTTACACCATGTCTACCTAAGTATCTTTGATACTGTGTCATGACCACATTTCTAAACTCTATAGTCTTTCTGCCTCACGGAGATT TGAGATAGGTCTTAATTTTTAAAATTAAACTTATTCATATTAAACAAAGACATATGAAGACTTTAGTAAGATATATTATGATATATTAAA GACCATTCTGCTCCAGGAAATTAGAAATTAACTACAGGCAAAATACACACATCCTAAAAAATGTTTTTATCATGTCAACAATAAAACACA AAGACATGAATTAAACATACACCCTTTTCCCGCTGTCTCATTAGGAAATTAAAAAACAATTCAGAGGTCTGGAGGAAAATGGAAATATTT AAAATGCTTTTTAGAACTGGGGAAGACAGGCCAAGCATGGTGGCTCATCCCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGCAGATC ACCAGAAATCAGGAGTTCGAGACCCCGCTGGCCAACAGGGCAAAACCCATCTCTACCAAAAATACAAAAATTAGCTGGGCGTGGTGGCAG GCACCTGTAATCCCAGCTGCTTGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGAGAGGCGGAGGTTGCAGTGAGCCGAGATTGCCCCA CTGCACTCTAGCCTGGGGGATAGAGTGAAACTCTACCTCCAAAAAAAAGAAAAGAAAAATAACTGAGGAAGACAGTTTTCACAGTGGGTC AGGTGAGCCTAGAATTATCGAATATCGTCCTTTAACCTATAGCTATGACTCTAGGTTAAAGCTCCTCCTCAGCTTGCTCCAGTCAAGAGT ATCTATGTATTTGAGCCATTTCCTGAAACCAGACTCTAGTGTTTCTGATCAGACCAGCTCAGTCTTTAAGTTTCTGAAGTTTGTTTGCTA >35780_35780_4_HCN1-SPEF2_HCN1_chr5_45645287_ENST00000303230_SPEF2_chr5_35667174_ENST00000509059_length(amino acids)=837AA_BP=283 MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQ YGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNV ASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLR LSRLIRYIHQWEEALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELF FNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELP SFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSD EVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEAL TGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRD QIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPE -------------------------------------------------------------- |
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Fusion Gene PPI Analysis for HCN1-SPEF2 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000282469 | 7 | 10 | 1324_1676 | 389.0 | 515.0 | IFT20 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000303129 | 0 | 13 | 1324_1676 | 0 | 620.0 | IFT20 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000356031 | 7 | 37 | 1324_1676 | 389.0 | 1823.0 | IFT20 | |
| Tgene | SPEF2 | chr5:45645287 | chr5:35667174 | ENST00000440995 | 7 | 37 | 1324_1676 | 389.0 | 1819.0 | IFT20 |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for HCN1-SPEF2 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for HCN1-SPEF2 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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