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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:HDAC4-CUL3 (FusionGDB2 ID:35831)

Fusion Gene Summary for HDAC4-CUL3

Fusion gene summary

Fusion gene information	Fusion gene name: HDAC4-CUL3
	Fusion gene ID: 35831
		Hgene	Tgene
	Gene symbol	HDAC4	CUL3
	Gene ID	9759	8452
	Gene name	histone deacetylase 4	cullin 3
	Synonyms	AHO3\|BDMR\|HA6116\|HD4\|HDAC-4\|HDAC-A\|HDACA	CUL-3\|PHA2E
	Cytomap	2q37.3	2q36.2
	Type of gene	protein-coding	protein-coding
	Description	histone deacetylase 4histone deacetylase A	cullin-3
	Modification date	20200329	20200327
	UniProtAcc	P56524	Q13618
	Ensembl transtripts involved in fusion gene	ENST00000345617, ENST00000541256, ENST00000543185, ENST00000553145,	ENST00000409096, ENST00000409777, ENST00000344951, ENST00000432260, ENST00000264414,
Fusion gene scores	* DoF score	13 X 10 X 9=1170	8 X 8 X 5=320
	# samples	15	9
	** MAII score	log2(15/1170*10)=-2.96347412397489 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/320*10)=-1.83007499855769 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: HDAC4 [Title/Abstract] AND CUL3 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	HDAC4(240048151)-CUL3(225422573), # samples:1
Anticipated loss of major functional domain due to fusion event.	HDAC4-CUL3 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF. HDAC4-CUL3 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. HDAC4-CUL3 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. HDAC4-CUL3 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	HDAC4	GO:0000122	negative regulation of transcription by RNA polymerase II	10869435\|16236793
Hgene	HDAC4	GO:0006338	chromatin remodeling	18850004
Hgene	HDAC4	GO:0006476	protein deacetylation	27708256
Hgene	HDAC4	GO:0016575	histone deacetylation	10869435
Hgene	HDAC4	GO:0033235	positive regulation of protein sumoylation	17696781
Hgene	HDAC4	GO:0034983	peptidyl-lysine deacetylation	18614528
Hgene	HDAC4	GO:0045892	negative regulation of transcription, DNA-templated	10869435
Hgene	HDAC4	GO:0070932	histone H3 deacetylation	12590135
Hgene	HDAC4	GO:0070933	histone H4 deacetylation	12590135
Tgene	CUL3	GO:0000209	protein polyubiquitination	19261606
Tgene	CUL3	GO:0006511	ubiquitin-dependent protein catabolic process	25401743\|27561354
Tgene	CUL3	GO:0006513	protein monoubiquitination	22358839
Tgene	CUL3	GO:0006888	ER to Golgi vesicle-mediated transport	22358839
Tgene	CUL3	GO:0016567	protein ubiquitination	17543862\|19782033\|19995937\|20389280\|23213400
Tgene	CUL3	GO:0031145	anaphase-promoting complex-dependent catabolic process	10500095
Tgene	CUL3	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	19261606\|19782033\|20389280
Tgene	CUL3	GO:0071630	nuclear protein quality control by the ubiquitin-proteasome system	27561354

Fusion gene breakpoints across HDAC4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across CUL3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	STAD	TCGA-VQ-A94O	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-

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Fusion Gene ORF analysis for HDAC4-CUL3

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-5UTR	ENST00000345617	ENST00000409096	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-5UTR	ENST00000345617	ENST00000409777	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-5UTR	ENST00000541256	ENST00000409096	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-5UTR	ENST00000541256	ENST00000409777	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-5UTR	ENST00000543185	ENST00000409096	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-5UTR	ENST00000543185	ENST00000409777	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-intron	ENST00000345617	ENST00000344951	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-intron	ENST00000345617	ENST00000432260	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-intron	ENST00000541256	ENST00000344951	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-intron	ENST00000541256	ENST00000432260	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-intron	ENST00000543185	ENST00000344951	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
5CDS-intron	ENST00000543185	ENST00000432260	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
Frame-shift	ENST00000543185	ENST00000264414	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
In-frame	ENST00000345617	ENST00000264414	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
In-frame	ENST00000541256	ENST00000264414	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
intron-3CDS	ENST00000553145	ENST00000264414	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
intron-5UTR	ENST00000553145	ENST00000409096	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
intron-5UTR	ENST00000553145	ENST00000409777	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
intron-intron	ENST00000553145	ENST00000344951	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-
intron-intron	ENST00000553145	ENST00000432260	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000345617	HDAC4	chr2	240048151	-	ENST00000264414	CUL3	chr2	225422573	-	8646	2310	783	4550	1255
ENST00000541256	HDAC4	chr2	240048151	-	ENST00000264414	CUL3	chr2	225422573	-	7853	1517	35	3757	1240

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000345617	ENST00000264414	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-	0.000588556	0.99941146
ENST00000541256	ENST00000264414	HDAC4	chr2	240048151	-	CUL3	chr2	225422573	-	0.000127956	0.99987197

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Fusion Genomic Features for HDAC4-CUL3

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

1-p

p (fusion gene breakpoint)

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for HDAC4-CUL3

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:240048151/chr2:225422573)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
HDAC4 P56524	CUL3 Q13618
FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed:27708256). {ECO:0000269\|PubMed:10523670, ECO:0000269\|PubMed:24413532, ECO:0000269\|PubMed:27708256}.	FUNCTION: Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component. BCR(KLHL42) is involved in ubiquitination of KATNA1. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, BRMS1, MACROH2A1 and DAXX, GLI2 and GLI3. Can also form a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL; these complexes have lower ubiquitin ligase activity. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27716508). BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; by mediating ubiquitination of WNK4 (PubMed:23387299, PubMed:23453970, PubMed:23576762). The BCR(KLHL20) E3 ubiquitin ligase complex is involved in interferon response and anterograde Golgi to endosome transport: it mediates both ubiquitination leading to degradation and 'Lys-33'-linked ubiquitination (PubMed:20389280, PubMed:21840486, PubMed:21670212, PubMed:24768539). The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB (PubMed:19995937). The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation (PubMed:23455478). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway (PubMed:29769719). The BCR(KLHL25) ubiquitin ligase complex is involved in translational homeostasis by mediating ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) (PubMed:22578813). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41 (PubMed:15983046). In concert with ATF2 and RBX1, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. The BCR(KCTD17) E3 ubiquitin ligase complex mediates ubiquitination and degradation of TCHP, a down-regulator of cilium assembly, thereby inducing ciliogenesis (PubMed:25270598). The BCR(KLHL24) E3 ubiquitin ligase complex mediates ubiquitination of KRT14, controls KRT14 levels during keratinocytes differentiation, and is essential for skin integrity (PubMed:27798626). The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (PubMed:23213400). The BCR(KEAP1) E3 ubiquitin ligase complex acts as a key sensor of oxidative and electrophilic stress by mediating ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed:15601839, PubMed:16006525). {ECO:0000269\|PubMed:10500095, ECO:0000269\|PubMed:11311237, ECO:0000269\|PubMed:15601839, ECO:0000269\|PubMed:15897469, ECO:0000269\|PubMed:15983046, ECO:0000269\|PubMed:16006525, ECO:0000269\|PubMed:16524876, ECO:0000269\|PubMed:17543862, ECO:0000269\|PubMed:18397884, ECO:0000269\|PubMed:19261606, ECO:0000269\|PubMed:19995937, ECO:0000269\|PubMed:20389280, ECO:0000269\|PubMed:21670212, ECO:0000269\|PubMed:21840486, ECO:0000269\|PubMed:22085717, ECO:0000269\|PubMed:22358839, ECO:0000269\|PubMed:22578813, ECO:0000269\|PubMed:22632832, ECO:0000269\|PubMed:23213400, ECO:0000269\|PubMed:23387299, ECO:0000269\|PubMed:23453970, ECO:0000269\|PubMed:23455478, ECO:0000269\|PubMed:23576762, ECO:0000269\|PubMed:24768539, ECO:0000269\|PubMed:25270598, ECO:0000269\|PubMed:26399832, ECO:0000269\|PubMed:27565346, ECO:0000269\|PubMed:27716508, ECO:0000269\|PubMed:27798626, ECO:0000269\|PubMed:29769719}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

HDAC4

chr2:240048151

chr2:225422573

ENST00000345617

67_177

506

1085.0

Coiled coil

Ontology_term=ECO:0000255

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

HDAC4

chr2:240048151

chr2:225422573

ENST00000345617

1051_1084

506

1085.0

Motif

Nuclear export signal

Hgene

HDAC4

chr2:240048151

chr2:225422573

ENST00000345617

655_1084

506

1085.0

Region

Note=Histone deacetylase

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Fusion Gene Sequence for HDAC4-CUL3

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>35831_35831_1_HDAC4-CUL3_HDAC4_chr2_240048151_ENST00000345617_CUL3_chr2_225422573_ENST00000264414_length(transcript)=8646nt_BP=2310nt
GGAGGTTGTGGGGCCGCCGCCGCGGAGCACCGTCCCCGCCGCCGCCCGAGCCCGAGCCCGAGCCCGCGCACCCGCCCGCGCCGCCGCCGC
CGCCGCCCGAACAGCCTCCCAGCCTGGGCCCCCGGCGGCGCCGTGGCCGCGTCCCGGCTGTCGCCGCCCGAGCCCGAGCCCGCGCGCCGG
CGGGTGGCGGCGCAGGCTGAGGAGATGCGGCGCGGAGCGCCGGAGCAGGGCTAGAGCCGGCCGCCGCCGCCCGCCGCGGTAAGCGCAGCC
CCGGCCCGGCGCCCGCGGGCCATTGTCCGCCGCCCGCCCCGCGCCCCGCGCAGCCTGCAGGCCTTGGAGCCCGCGGCAGGTGGACGCCGC
CGGTCCACACCCGCCCCGCGCGCGGCCGTGGGAGGCGGGGGCCAGCGCTGGCCGCGCGCCGTGGGACCCGCCGGTCCCCAGGGCCGCCCG
GCCCCTTCTGGACCTTTCCACCCGCGCCGCGAGGCGGCTTCGCCCGCCGGGGCGGGGGCGCGGGGGTGGGCACGGCAGGCAGCGGCGCCG
TCTCCCGGTGCGGGGCCCGCGCCCCCCGAGCAGGTTCATCTGCAGAAGCCAGCGGACGCCTCTGTTCAACTTGTGGGTTACCTGGCTCAT
GAGACCTTGCCGGCGAGGCTCGGCGCTTGAACGTCTGTGACCCAGCCCTCACCGTCCCGGTACTTGTATGTGTTGGTGGGAGTTTGGAGC
TCGTTGGAGCTATCGTTTCCGTGGAAATTTTGAGCCATTTCGAATCACTTAAAGGAGTGGACATTGCTAGCAATGAGCTCCCAAAGCCAT
CCAGATGGACTTTCTGGCCGAGACCAGCCAGTGGAGCTGCTGAATCCTGCCCGCGTGAACCACATGCCCAGCACGGTGGATGTGGCCACG
GCGCTGCCTCTGCAAGTGGCCCCCTCGGCAGTGCCCATGGACCTGCGCCTGGACCACCAGTTCTCACTGCCTGTGGCAGAGCCGGCCCTG
CGGGAGCAGCAGCTGCAGCAGGAGCTCCTGGCGCTCAAGCAGAAGCAGCAGATCCAGAGGCAGATCCTCATCGCTGAGTTCCAGAGGCAG
CACGAGCAGCTCTCCCGGCAGCACGAGGCGCAGCTCCACGAGCACATCAAGCAACAACAGGAGATGCTGGCCATGAAGCACCAGCAGGAG
CTGCTGGAACACCAGCGGAAGCTGGAGAGGCACCGCCAGGAGCAGGAGCTGGAGAAGCAGCACCGGGAGCAGAAGCTGCAGCAGCTCAAG
AACAAGGAGAAGGGCAAAGAGAGTGCCGTGGCCAGCACAGAAGTGAAGATGAAGTTACAAGAATTTGTCCTCAATAAAAAGAAGGCGCTG
GCCCACCGGAATCTGAACCACTGCATTTCCAGCGACCCTCGCTACTGGTACGGGAAAACGCAGCACAGTTCCCTTGACCAGAGTTCTCCA
CCCCAGAGCGGAGTGTCGACCTCCTATAACCACCCGGTCCTGGGAATGTACGACGCCAAAGATGACTTCCCTCTTAGGAAAACAGCTTCT
GAACCGAATCTGAAATTACGGTCCAGGCTAAAGCAGAAAGTGGCCGAAAGACGGAGCAGCCCCCTGTTACGCAGGAAAGACGGGCCAGTG
GTCACTGCTCTAAAAAAGCGTCCGTTGGATGTCACAGACTCCGCGTGCAGCAGCGCCCCAGGCTCCGGACCCAGCTCACCCAACAACAGC
TCCGGGAGCGTCAGCGCGGAGAACGGTATCGCGCCCGCCGTCCCCAGCATCCCGGCGGAGACGAGTTTGGCGCACAGACTTGTGGCACGA
GAAGGCTCGGCCGCTCCACTTCCCCTCTACACATCGCCATCCTTGCCCAACATCACGCTGGGCCTGCCTGCCACCGGCCCCTCTGCGGGC
ACGGCGGGCCAGCAGGACGCCGAGAGACTCACCCTTCCCGCCCTCCAGCAGAGGCTCTCCCTTTTCCCCGGCACCCACCTCACTCCCTAC
CTGAGCACCTCGCCCTTGGAGCGGGACGGAGGGGCAGCGCACAGCCCTCTTCTGCAGCACATGGTCTTACTGGAGCAGCCGCCGGCACAA
GCACCCCTCGTCACAGGCCTGGGAGCACTGCCCCTCCACGCACAGTCCTTGGTTGGTGCAGACCGGGTGTCCCCCTCCATCCACAAGCTG
CGGCAGCACCGCCCACTGGGGCGGACCCAGTCGGCCCCGCTGCCCCAGAACGCCCAGGCTCTGCAGCACCTGGTCATCCAGCAGCAGCAT
CAGCAGTTTCTGGAGAAACACAAGCAGCAGTTCCAGCAGCAGCAACTGCAGATGAACAAGATGACCATGGATGAAAAATATGTAAACAGC
ATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATAT
ACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAAATAAGGTGCGAGAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGAGACATA
CTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTTTTCGAGATCAAGTTGTA
CGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGAGGCGCA
ATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATG
TCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGAATTAAT
GAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCAC
ATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTACAAGTTA
TTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAA
GGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTCAACAAT
GACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATT
GATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGGTTTATG
CAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAA
AACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACA
ACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGGTGCTCACGACAGGATAT
TGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTAGCCAAA
CACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAGTTAAAAAGGAAGATGGA
TCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACCATATTA
ATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACAGATATCCCTGAAAGAGAGCTTGTTAGAGCCCTACAG
TCCCTCGCCTGTGGTAAACCAACACAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAATGGTCATATATTTACAGTTAAT
GATCAATTCACATCCAAACTACACAGAGTCAAGATTCAAACAGTTGCTGCCAAACAAGGTGAATCCGACCCAGAGAGGAAAGAAACAAGG
CAGAAAGTAGACGACGACAGAAAACATGAGATAGAAGCTGCTATAGTGCGGATAATGAAATCTAGAAAGAAGATGCAGCACAATGTTCTA
GTAGCGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAA
TATTTGGCACGAACACCTGAGGATCGCAAAGTATACACATATGTAGCATAAAATGCGTTCAGAAATTTGATTTATTCTTGGACTGTACTC
TTCGCATGGACTGGGAAGTTCTTTTAAATCATTAAATATTAAGACGACCATCTCTTCTATTAAATTACAGTACATGTTCTAGACCATTGA
GATCAAGCCTTTACTCCCTTTGAGAGTTTCCAACATCAGTTGATTGAGCTTCAGGCTTTACAACGTTTATCCCTGTAGAGATCATCTTTA
CAGTTCCTCGGGAAAATGTGAATGTGCTGCGTTTTGTTTTCTTTACTGTATGAAAACAGGAAAATAAAAGAGAAATTTAGAAAATACAGC
TCATTACAATAAAATTGTTGGATTTCATTTCCCCAGGTCTTCAGTGTTGATGTAAATGTGTTTTGTAGTGTTGCTTAGCACTTTGCGCAT
TGTGTAAGTTGGGTAACAAAAATGGCAAAAGAAATGCAGATTTCCCAGTTATCTTGCTCTGAAGACATTTCTTTAGCTAGTCTTATTTAA
TTTTAAAGGTTTGATAAATATACAAAGACCCAAGCATACAATTTGAGCATGCTGTATTCTACCACTTGCACTTACTCATCTTCTAGCCTT
ATGACCAGGAACCTTTGTTTTGGGCTCAAGTACACATCCTGATTTGGTTTCTCCCTGTTGTAATTTGAGATTTCCCAAATTAAGTCTTAT
AATGATTAGGTTTTGTTCTGAGTTATTTCTAGAGAAAGAAAGTATTTTAGTATGTGTAAATTGTACAATAATTTTTTTGCTGTTGTACTC
ACTGCTAATAGTGGATTGTATAGGGTGGTGTTTTTATTTCATTTATTGTAGACAAAAATAATGAATTTAGTATACACATACCCACTAATT
CAAGTATGTCAGAGCACAAAGTTGGCAGCTGGTTATATTTAATTCAGCCCCTCTGAAAAGCACATACAGTCTATACTTTGTTTATAAATA
CCCTAGGTCCTCCCTGCCCCTAAATATATATTTTGGTGAAGCTGTGGTGCACTATTAATTTTCTGTTTCAAAATGCAATCTAAAGATGCA
ATAAATATGATGACCTTGATAGTTTTAATGAAATCTTCAATTCTTGCAGCTGTGTTTTGGAAAGTGATTAAGCAATATTTCTCAAACCTG
ATGATTCTTGGATATTTAGCTATTGTCCTCCAAAGAGTCATCGTTTCACGTTTTCAAACATGTGCTTTGTGCTGAAGATTCTTGTCAGAT
ATCCTGTGCTTCCAGAGTATTTTTATCTTCAATTTTATTTAATTTTGTTTCATTTTGTTTTCATAAGACAATGTTTCAGATATGTAATGG
GGCAAACCACTATAGATTTCTGTTGGATAGAAAAATGAAATGTTACTAATAAGTTTAAATTGACCCTTCGTTGACTATCAGTACTGTTGA
TACATTTATTAGAATGTATGTCTCAGAAGATGGTTTCTGTTTAAATTTATGGTCAAGTTACAACAACTGAATTCTAATATGGATTAAAAA
CTCCACATAAATATTTTTCCCCATGCGTCTTGGGTTATAATTTTCCTTTTAGACTTGCAAATATGCTTTTATTGCAATTTTGTCTCCATC
TGTACTGTAGAATTACAAGCCTTGTGCTCTTTATCTCTACATTTTCCACTTCTACATGATGCAATTAAGATGCCAATGAAAAGTATTGTG
AACATTATTCTCAGGACACTTTTACCATACTTGAGTAGTACTAAGTCAGGTGTAACATGGAAAGCTAAGGGTTTCACCTTCTGATTTTGA
CATAATTGGAAATTTTCTATAATGGTAAGATGTTTATAGGAAAGTAATGGTAGTTCATGTTTGAGAATTTTTTTATTGCTTTCATTTCCC
AGTTAAATGATATTTAACACCTGGGAAAACAGGACAGCTCAGATTGTCGAGTTAGTAAACATGAGCGATGTAAAAACTTTATTTGAAATC
TCCTAAGTGAGACATTAAACATTTTAGTGCTAGTTTAAGCCATTAAAAGTAAAGTTTAAGTTTACGTCATTGTCTCAAGTGTGGCTGTAA
AAATCAGATCTAGAGTTTAGAGACATACTTGTTAACTTTGGAGCACAGGTTTGATATTTATGCTTCTAAATACATTAAAACATTTCTGAA
TTGCAGTTGAATGGTTGGAGATTGCTGGCGATGTGATGCTGCACTGAAAAAACTGGTCACACAAAATGTTTATATGTTTGACTGCATAAG
TATTATTTTAGGTCATAAAGGTTATAACAAAATACATTTAACATTGCTTTCTTTTTAGCAAGAGGTCACAGATGCTATTTTTTCTCAGTT
TTTAATGCTTTAATTTGAAAGAAAAATGCATCCTAACCATTCCATCCTGTTGGTGCAGAGAAATTTTGGTGATTTATTAGAAGACTTTTA
AGGAGGGTGGATACATAGTTTTTCAACTGCAAAAATCATGTCATCTCTTCAGTAACTGTTAAAGTGTAATTCTCTGCCTTCATTATCAAG
TCCAGGACAGTGTTTATTTTGAATCGTTAACATTATTTAAAGACTGAAAATTCATTGCAATTAGGGAACGCACAGCCTCACAAAAAGTGG
TTGTGATTGCAAGGATTCTTTATGTCAGTGAACTTAAAAAGGGAATGGGGGAAGAGATGCATGCCTTTCTAAAGGTATGCTAGAGTCACC
TGTGTAGCATTGTAATTATGTACTATCAAACCCCCAAGATGCTGATGATAGGCCTTCTCTGTAAGAATCCTTCTCATAGTGAAGCACTGT
TGATGTTTTGTTCACCGGATATGTTGGGTAGCAGAAAAGGCCTAGAACCAGTTTTAAGATTATGCCTACATCTCTAGAAGACTAGTACAC
TGGCATCATCTTAGGTTTCTAAATATTATTTAATTCCTTAATTTGGAAAGCAGTTTTAATGTTTACAGTTTAAGATACTCATTTTCAGTA
ACTAGTGTTAAAGATTTTTATCTTTGCCTGTCTACTGTAACTTAAGACTTTCATAGAATAATTTTTTATAGAGTATATGGTATATTGTGT
CAATCTTGGAAGGAAAGCTGTTGCATATCAATTGTAGGTCATGTATTTTACTATATGTTGCATAGTATTTTGCATTCTGTAAATACGAAT
GTTAAAACATTTCAGGTCAGCAAAATTAGAAGTCATTTCTAGTCCTCAAAGCAGAAATAATTAGAAATGTTAACATTTAAGTGCTTATCA
TATGCCAGATACTATTCTGAATATTTTATGTATATTCATTCATTTAGTTGTCATATTGGCTTTATGTGACATAACTGTCATTATACCAGT
GTTGTCCAAGGTTGCTAGCAGGAAATGGCAGAGCCAGGAGTTGGACCTAGGTTTAATCCTATCCATATTCCATGTTACAAATTGACATGA
TTTTCCATATTAAAAAATTTTCATTTATTTTGTACCTCATTCACATGAATAGTAATGTGACAAACTAAAGTTAATACATGAAGCATTGCA
ATATGGAATTTTGCACATTTTTGTTAGCTTTACATTATTTGCTGATACCAACTTTTGGTAGGAAGGAAAACAGGGAAGGTGATTGCTGCT
ATTCTGGAATGTGTCCATTGAAAAGAAAATAGGCGCTTTGTCTCCTACAATCTTAGAGAAAGTTTTACATACTTAAGTGGGTACCACAGA
TAGGCATGTACCTTAAATTCAAATCTACAGTTTTACAAAATGTTTTCCTTTGTGTGCCTTTCCTATGTCAAATTAGCCAGAAGGAAGAAG
AGACGTTAAGAATGGCAGATAAATTTAACAGGTAATGATACAGAGATTGGCCATTACGCCAGCTCTCAGGAGAAAAACAACTAGGATGTC
TTGAAACCCGTGCATTTAATTTTAGAAATGGCAAATTTGTAAGCGTGTCATTTAAGTAATTTGATGCTCAGTTTTAGGGTCTAAGTTGAC
CTCGAGTGAGCCATGCAAAAATAGCTTTAAAATTATACATGAGCAGCTCAAAGTAAACGTGTATTTTTTAGTAACAATGAAATTGTCAAA
TTTCAAGATTTTAATAACAGGACAGTTCAACTAATAAACTTTATTGCATACAAGATTTATTGCAATGTGGGAAAATTAAAATTCTCAGTC

>35831_35831_1_HDAC4-CUL3_HDAC4_chr2_240048151_ENST00000345617_CUL3_chr2_225422573_ENST00000264414_length(amino acids)=1255AA_BP=509
MLAMSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQKQQIQRQ
ILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQE
FVLNKKKALAHRNLNHCISSDPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSP
LLRRKDGPVVTALKKRPLDVTDSACSSAPGSGPSSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLG
LPATGPSAGTAGQQDAERLTLPALQQRLSLFPGTHLTPYLSTSPLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGAD
RVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQQLQMNKMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLS
FEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLG
LIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVY
IKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLRE
QGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILD
KAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDL
TVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQ
VSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGE

--------------------------------------------------------------
>35831_35831_2_HDAC4-CUL3_HDAC4_chr2_240048151_ENST00000541256_CUL3_chr2_225422573_ENST00000264414_length(transcript)=7853nt_BP=1517nt
AGTTTTCGGCAGAGTCGCGTGTCTGAGAAGTGACGCTGGGATGTCCCTGGGCTGTGCCAGGAGCAGTGCTGGCTCAGATGGATGTGGCCA
CGGCGCTGCCTCTGCAAGTGGCCCCCTCGGCAGTGCCCATGGACCTGCGCCTGGACCACCAGTTCTCACTGCCTGTGGCAGAGCCGGCCC
TGCGGGAGCAGCAGCTGCAGCAGGAGCTCCTGGCGCTCAAGCAGAAGCAGCAGATCCAGAGGCAGATCCTCATCGCTGAGTTCCAGAGGC
AGCACGAGCAGCTCTCCCGGCAGCACGAGGCGCAGCTCCACGAGCACATCAAGCAACAACAGGAGATGCTGGCCATGAAGCACCAGCAGG
AGCTGCTGGAACACCAGCGGAAGCTGGAGAGGCACCGCCAGGAGCAGGAGCTGGAGAAGCAGCACCGGGAGCAGAAGCTGCAGCAGCTCA
AGAACAAGGAGAAGGGCAAAGAGAGTGCCGTGGCCAGCACAGAAGTGAAGATGAAGTTACAAGAATTTGTCCTCAATAAAAAGAAGGCGC
TGGCCCACCGGAATCTGAACCACTGCATTTCCAGCGACCCTCGCTACTGGTACGGGAAAACGCAGCACAGTTCCCTTGACCAGAGTTCTC
CACCCCAGAGCGGAGTGTCGACCTCCTATAACCACCCGGTCCTGGGAATGTACGACGCCAAAGATGACTTCCCTCTTAGGAAAACAGCTT
CTGAACCGAATCTGAAATTACGGTCCAGGCTAAAGCAGAAAGTGGCCGAAAGACGGAGCAGCCCCCTGTTACGCAGGAAAGACGGGCCAG
TGGTCACTGCTCTAAAAAAGCGTCCGTTGGATGTCACAGACTCCGCGTGCAGCAGCGCCCCAGGCTCCGGACCCAGCTCACCCAACAACA
GCTCCGGGAGCGTCAGCGCGGAGAACGGTATCGCGCCCGCCGTCCCCAGCATCCCGGCGGAGACGAGTTTGGCGCACAGACTTGTGGCAC
GAGAAGGCTCGGCCGCTCCACTTCCCCTCTACACATCGCCATCCTTGCCCAACATCACGCTGGGCCTGCCTGCCACCGGCCCCTCTGCGG
GCACGGCGGGCCAGCAGGACGCCGAGAGACTCACCCTTCCCGCCCTCCAGCAGAGGCTCTCCCTTTTCCCCGGCACCCACCTCACTCCCT
ACCTGAGCACCTCGCCCTTGGAGCGGGACGGAGGGGCAGCGCACAGCCCTCTTCTGCAGCACATGGTCTTACTGGAGCAGCCGCCGGCAC
AAGCACCCCTCGTCACAGACTGGTATCTTTCAGGCCTGGGAGCACTGCCCCTCCACGCACAGTCCTTGGTTGGTGCAGACCGGGTGTCCC
CCTCCATCCACAAGCTGCGGCAGCACCGCCCACTGGGGCGGACCCAGTCGGCCCCGCTGCCCCAGAACGCCCAGGCTCTGCAGCACCTGG
TCATCCAGCAGCAGCATCAGCAGTTTCTGGAGAAACACAAGCAGCAGTTCCAGCAGCAGCAACTGCAGATGAACAAGATGACCATGGATG
AAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGC
TCTATAGAAATGCATATACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACCGAACATCTCATAA
ATAAGGTGCGAGAAGATGTACTAAATTCATTGAATAACAACTTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGG
TGATGATTAGAGACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTACAATTTGGGATTAATTATTT
TTCGAGATCAAGTTGTACGTTATGGGTGTATTAGGGATCATCTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAG
TCGTAGACAGAGGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTCTATGAAGAAGATTTTGAGG
CTCCTTTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAG
TAGAAGCTAGAATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATTGTAAAGGTGGTTGAAAGGG
AACTCATTTCCAAGCACATGAAGACTATAGTAGAAATGGAGAATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTG
GTTGCATGTACAAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTATTTGAGGGAGCAAGGTAAAG
CTCTTGTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCC
TGGAATCATTCAACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTCAACTCCAGGTCTCCTGAAT
ACCTCTCATTATTTATTGATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGG
TCCTTTTTAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTTCTCACAAATAAAAGTGTTT
CTGATGACTCTGAAAAAAACATGATATCTAAGTTAAAGACTGAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATA
TGAGCATCTCAAACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGTGTTGATCTTACAGTCCGGG
TGCTCACGACAGGATATTGGCCCACTCAGTCAGCCACACCAAAGTGCAACATCCCACCAGCACCAAGACATGCTTTTGAGATATTCAGAA
GGTTCTACTTAGCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAATGCCACATTTTATGGACCAG
TTAAAAAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCACAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTT
TCCAGATGACCATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACAGATATCCCTGAAAGAGAGC
TTGTTAGAGCCCTACAGTCCCTCGCCTGTGGTAAACCAACACAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAATGGTC
ATATATTTACAGTTAATGATCAATTCACATCCAAACTACACAGAGTCAAGATTCAAACAGTTGCTGCCAAACAAGGTGAATCCGACCCAG
AGAGGAAAGAAACAAGGCAGAAAGTAGACGACGACAGAAAACATGAGATAGAAGCTGCTATAGTGCGGATAATGAAATCTAGAAAGAAGA
TGCAGCACAATGTTCTAGTAGCGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTGAAG
GACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGATCGCAAAGTATACACATATGTAGCATAAAATGCGTTCAGAAATTTGATTT
ATTCTTGGACTGTACTCTTCGCATGGACTGGGAAGTTCTTTTAAATCATTAAATATTAAGACGACCATCTCTTCTATTAAATTACAGTAC
ATGTTCTAGACCATTGAGATCAAGCCTTTACTCCCTTTGAGAGTTTCCAACATCAGTTGATTGAGCTTCAGGCTTTACAACGTTTATCCC
TGTAGAGATCATCTTTACAGTTCCTCGGGAAAATGTGAATGTGCTGCGTTTTGTTTTCTTTACTGTATGAAAACAGGAAAATAAAAGAGA
AATTTAGAAAATACAGCTCATTACAATAAAATTGTTGGATTTCATTTCCCCAGGTCTTCAGTGTTGATGTAAATGTGTTTTGTAGTGTTG
CTTAGCACTTTGCGCATTGTGTAAGTTGGGTAACAAAAATGGCAAAAGAAATGCAGATTTCCCAGTTATCTTGCTCTGAAGACATTTCTT
TAGCTAGTCTTATTTAATTTTAAAGGTTTGATAAATATACAAAGACCCAAGCATACAATTTGAGCATGCTGTATTCTACCACTTGCACTT
ACTCATCTTCTAGCCTTATGACCAGGAACCTTTGTTTTGGGCTCAAGTACACATCCTGATTTGGTTTCTCCCTGTTGTAATTTGAGATTT
CCCAAATTAAGTCTTATAATGATTAGGTTTTGTTCTGAGTTATTTCTAGAGAAAGAAAGTATTTTAGTATGTGTAAATTGTACAATAATT
TTTTTGCTGTTGTACTCACTGCTAATAGTGGATTGTATAGGGTGGTGTTTTTATTTCATTTATTGTAGACAAAAATAATGAATTTAGTAT
ACACATACCCACTAATTCAAGTATGTCAGAGCACAAAGTTGGCAGCTGGTTATATTTAATTCAGCCCCTCTGAAAAGCACATACAGTCTA
TACTTTGTTTATAAATACCCTAGGTCCTCCCTGCCCCTAAATATATATTTTGGTGAAGCTGTGGTGCACTATTAATTTTCTGTTTCAAAA
TGCAATCTAAAGATGCAATAAATATGATGACCTTGATAGTTTTAATGAAATCTTCAATTCTTGCAGCTGTGTTTTGGAAAGTGATTAAGC
AATATTTCTCAAACCTGATGATTCTTGGATATTTAGCTATTGTCCTCCAAAGAGTCATCGTTTCACGTTTTCAAACATGTGCTTTGTGCT
GAAGATTCTTGTCAGATATCCTGTGCTTCCAGAGTATTTTTATCTTCAATTTTATTTAATTTTGTTTCATTTTGTTTTCATAAGACAATG
TTTCAGATATGTAATGGGGCAAACCACTATAGATTTCTGTTGGATAGAAAAATGAAATGTTACTAATAAGTTTAAATTGACCCTTCGTTG
ACTATCAGTACTGTTGATACATTTATTAGAATGTATGTCTCAGAAGATGGTTTCTGTTTAAATTTATGGTCAAGTTACAACAACTGAATT
CTAATATGGATTAAAAACTCCACATAAATATTTTTCCCCATGCGTCTTGGGTTATAATTTTCCTTTTAGACTTGCAAATATGCTTTTATT
GCAATTTTGTCTCCATCTGTACTGTAGAATTACAAGCCTTGTGCTCTTTATCTCTACATTTTCCACTTCTACATGATGCAATTAAGATGC
CAATGAAAAGTATTGTGAACATTATTCTCAGGACACTTTTACCATACTTGAGTAGTACTAAGTCAGGTGTAACATGGAAAGCTAAGGGTT
TCACCTTCTGATTTTGACATAATTGGAAATTTTCTATAATGGTAAGATGTTTATAGGAAAGTAATGGTAGTTCATGTTTGAGAATTTTTT
TATTGCTTTCATTTCCCAGTTAAATGATATTTAACACCTGGGAAAACAGGACAGCTCAGATTGTCGAGTTAGTAAACATGAGCGATGTAA
AAACTTTATTTGAAATCTCCTAAGTGAGACATTAAACATTTTAGTGCTAGTTTAAGCCATTAAAAGTAAAGTTTAAGTTTACGTCATTGT
CTCAAGTGTGGCTGTAAAAATCAGATCTAGAGTTTAGAGACATACTTGTTAACTTTGGAGCACAGGTTTGATATTTATGCTTCTAAATAC
ATTAAAACATTTCTGAATTGCAGTTGAATGGTTGGAGATTGCTGGCGATGTGATGCTGCACTGAAAAAACTGGTCACACAAAATGTTTAT
ATGTTTGACTGCATAAGTATTATTTTAGGTCATAAAGGTTATAACAAAATACATTTAACATTGCTTTCTTTTTAGCAAGAGGTCACAGAT
GCTATTTTTTCTCAGTTTTTAATGCTTTAATTTGAAAGAAAAATGCATCCTAACCATTCCATCCTGTTGGTGCAGAGAAATTTTGGTGAT
TTATTAGAAGACTTTTAAGGAGGGTGGATACATAGTTTTTCAACTGCAAAAATCATGTCATCTCTTCAGTAACTGTTAAAGTGTAATTCT
CTGCCTTCATTATCAAGTCCAGGACAGTGTTTATTTTGAATCGTTAACATTATTTAAAGACTGAAAATTCATTGCAATTAGGGAACGCAC
AGCCTCACAAAAAGTGGTTGTGATTGCAAGGATTCTTTATGTCAGTGAACTTAAAAAGGGAATGGGGGAAGAGATGCATGCCTTTCTAAA
GGTATGCTAGAGTCACCTGTGTAGCATTGTAATTATGTACTATCAAACCCCCAAGATGCTGATGATAGGCCTTCTCTGTAAGAATCCTTC
TCATAGTGAAGCACTGTTGATGTTTTGTTCACCGGATATGTTGGGTAGCAGAAAAGGCCTAGAACCAGTTTTAAGATTATGCCTACATCT
CTAGAAGACTAGTACACTGGCATCATCTTAGGTTTCTAAATATTATTTAATTCCTTAATTTGGAAAGCAGTTTTAATGTTTACAGTTTAA
GATACTCATTTTCAGTAACTAGTGTTAAAGATTTTTATCTTTGCCTGTCTACTGTAACTTAAGACTTTCATAGAATAATTTTTTATAGAG
TATATGGTATATTGTGTCAATCTTGGAAGGAAAGCTGTTGCATATCAATTGTAGGTCATGTATTTTACTATATGTTGCATAGTATTTTGC
ATTCTGTAAATACGAATGTTAAAACATTTCAGGTCAGCAAAATTAGAAGTCATTTCTAGTCCTCAAAGCAGAAATAATTAGAAATGTTAA
CATTTAAGTGCTTATCATATGCCAGATACTATTCTGAATATTTTATGTATATTCATTCATTTAGTTGTCATATTGGCTTTATGTGACATA
ACTGTCATTATACCAGTGTTGTCCAAGGTTGCTAGCAGGAAATGGCAGAGCCAGGAGTTGGACCTAGGTTTAATCCTATCCATATTCCAT
GTTACAAATTGACATGATTTTCCATATTAAAAAATTTTCATTTATTTTGTACCTCATTCACATGAATAGTAATGTGACAAACTAAAGTTA
ATACATGAAGCATTGCAATATGGAATTTTGCACATTTTTGTTAGCTTTACATTATTTGCTGATACCAACTTTTGGTAGGAAGGAAAACAG
GGAAGGTGATTGCTGCTATTCTGGAATGTGTCCATTGAAAAGAAAATAGGCGCTTTGTCTCCTACAATCTTAGAGAAAGTTTTACATACT
TAAGTGGGTACCACAGATAGGCATGTACCTTAAATTCAAATCTACAGTTTTACAAAATGTTTTCCTTTGTGTGCCTTTCCTATGTCAAAT
TAGCCAGAAGGAAGAAGAGACGTTAAGAATGGCAGATAAATTTAACAGGTAATGATACAGAGATTGGCCATTACGCCAGCTCTCAGGAGA
AAAACAACTAGGATGTCTTGAAACCCGTGCATTTAATTTTAGAAATGGCAAATTTGTAAGCGTGTCATTTAAGTAATTTGATGCTCAGTT
TTAGGGTCTAAGTTGACCTCGAGTGAGCCATGCAAAAATAGCTTTAAAATTATACATGAGCAGCTCAAAGTAAACGTGTATTTTTTAGTA
ACAATGAAATTGTCAAATTTCAAGATTTTAATAACAGGACAGTTCAACTAATAAACTTTATTGCATACAAGATTTATTGCAATGTGGGAA

>35831_35831_2_HDAC4-CUL3_HDAC4_chr2_240048151_ENST00000541256_CUL3_chr2_225422573_ENST00000264414_length(amino acids)=1240AA_BP=494
MGCPWAVPGAVLAQMDVATALPLQVAPSAVPMDLRLDHQFSLPVAEPALREQQLQQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQ
LHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISS
DPRYWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDV
TDSACSSAPGSGPSSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPSLPNITLGLPATGPSAGTAGQQDAERLT
LPALQQRLSLFPGTHLTPYLSTSPLERDGGAAHSPLLQHMVLLEQPPAQAPLVTDWYLSGLGALPLHAQSLVGADRVSPSIHKLRQHRPL
GRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQQLQMNKMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHK
HGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIR
DHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERV
MHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNP
VDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVF
ERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSA
TPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNR
EKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDD

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Top

Fusion Gene PPI Analysis for HDAC4-CUL3

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Still interaction with
Hgene	HDAC4	chr2:240048151	chr2:225422573	ENST00000345617	-	12	27	118_313	506.0	1085.0	MEF2A
Tgene	CUL3	chr2:240048151	chr2:225422573	ENST00000344951		0	15	2_41	0	703.0	KLHL18
Tgene	CUL3	chr2:240048151	chr2:225422573	ENST00000409096		0	16	2_41	0	745.0	KLHL18
Tgene	CUL3	chr2:240048151	chr2:225422573	ENST00000409777		0	16	2_41	0	745.0	KLHL18

- Lost PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	CUL3	chr2:240048151	chr2:225422573	ENST00000264414		0	16	2_41	22.0	769.0	KLHL18

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs for HDAC4-CUL3

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

Top

Related Diseases for HDAC4-CUL3

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source