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Fusion Gene Summary | |
Fusion Gene ORF analysis | |
Fusion Genomic Features | |
Fusion Protein Features | |
Fusion Gene Sequence | |
Fusion Gene PPI analysis | |
Related Drugs | |
Related Diseases |
Fusion gene:HNRNPM-MCOLN1 (FusionGDB2 ID:37281) |
Fusion Gene Summary for HNRNPM-MCOLN1 |
Fusion gene summary |
Fusion gene information | Fusion gene name: HNRNPM-MCOLN1 | Fusion gene ID: 37281 | Hgene | Tgene | Gene symbol | HNRNPM | MCOLN1 | Gene ID | 4670 | 57192 |
Gene name | heterogeneous nuclear ribonucleoprotein M | mucolipin 1 | |
Synonyms | CEAR|HNRNPM4|HNRPM|HNRPM4|HTGR1|NAGR1|hnRNP M | MG-2|ML1|ML4|MLIV|MST080|MSTP080|TRP-ML1|TRPM-L1|TRPML1 | |
Cytomap | 19p13.2 | 19p13.2 | |
Type of gene | protein-coding | protein-coding | |
Description | heterogeneous nuclear ribonucleoprotein MCEA receptorN-acetylglucosamine receptor 1heterogenous nuclear ribonucleoprotein M4hnRNA-binding protein M4 | mucolipin-1mucolipidinmucolipidosis type IV proteintransient receptor potential channel mucolipin 1 | |
Modification date | 20200322 | 20200320 | |
UniProtAcc | P52272 | Q9GZU1 | |
Ensembl transtripts involved in fusion gene | ENST00000325495, ENST00000348943, ENST00000602219, | ENST00000264079, | |
Fusion gene scores | * DoF score | 16 X 12 X 10=1920 | 5 X 6 X 3=90 |
# samples | 19 | 6 | |
** MAII score | log2(19/1920*10)=-3.33703498727757 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(6/90*10)=-0.584962500721156 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context | PubMed: HNRNPM [Title/Abstract] AND MCOLN1 [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint | HNRNPM(8527465)-MCOLN1(7592406), # samples:3 | ||
Anticipated loss of major functional domain due to fusion event. | HNRNPM-MCOLN1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. HNRNPM-MCOLN1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
Partner | Gene | GO ID | GO term | PubMed ID |
Tgene | MCOLN1 | GO:0051289 | protein homotetramerization | 29019983 |
Tgene | MCOLN1 | GO:0070588 | calcium ion transmembrane transport | 29019983 |
Fusion gene breakpoints across HNRNPM (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene breakpoints across MCOLN1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChimerDB4 | UCS | TCGA-N8-A4PN-01A | HNRNPM | chr19 | 8527465 | - | MCOLN1 | chr19 | 7592406 | + |
ChimerDB4 | UCS | TCGA-N8-A4PN-01A | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592406 | + |
ChimerDB4 | UCS | TCGA-N8-A4PN | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + |
ChimerDB4 | UCS | TCGA-N8-A4PN | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592406 | + |
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Fusion Gene ORF analysis for HNRNPM-MCOLN1 |
Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
Frame-shift | ENST00000325495 | ENST00000264079 | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592406 | + |
Frame-shift | ENST00000325495 | ENST00000264079 | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + |
Frame-shift | ENST00000348943 | ENST00000264079 | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592406 | + |
Frame-shift | ENST00000348943 | ENST00000264079 | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + |
intron-3CDS | ENST00000602219 | ENST00000264079 | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592406 | + |
intron-3CDS | ENST00000602219 | ENST00000264079 | HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + |
ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for HNRNPM-MCOLN1 |
FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + | 5.72E-06 | 0.9999943 |
HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + | 5.72E-06 | 0.9999943 |
HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + | 5.72E-06 | 0.9999943 |
HNRNPM | chr19 | 8527465 | + | MCOLN1 | chr19 | 7592405 | + | 5.72E-06 | 0.9999943 |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for HNRNPM-MCOLN1 |
Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:8527465/:7592406) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
Main function of each fusion partner protein. (from UniProt) |
Hgene | Tgene |
HNRNPM | MCOLN1 |
FUNCTION: Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. | FUNCTION: Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis. Proposed to play a major role in Ca(2+) release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy (PubMed:11013137, PubMed:12459486, PubMed:15336987, PubMed:14749347, PubMed:29019983, PubMed:27623384). Required for efficient uptake of large particles in macrophages in which Ca(2+) release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion (By similarity). Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events (PubMed:16978393). By mediating lysosomal Ca(2+) release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels (PubMed:27787197, PubMed:25733853). Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS-induced TFEB activation and autophagy (PubMed:27357649). Functions as a Fe(2+) permeable channel in late endosomes and lysosomes (PubMed:18794901). Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163 (PubMed:25130899) In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells (By similarity). {ECO:0000250|UniProtKB:Q99J21, ECO:0000269|PubMed:12459486, ECO:0000269|PubMed:14749347, ECO:0000269|PubMed:15336987, ECO:0000269|PubMed:16978393, ECO:0000269|PubMed:18794901, ECO:0000269|PubMed:25130899, ECO:0000269|PubMed:25733853, ECO:0000269|PubMed:27357649, ECO:0000269|PubMed:27623384, ECO:0000269|PubMed:27787197, ECO:0000269|PubMed:29019983, ECO:0000305|PubMed:11013137}.; FUNCTION: May contribute to cellular lipase activity within the late endosomal pathway or at the cell surface which may be involved in processes of membrane reshaping and vesiculation, especially the growth of tubular structures. However, it is not known, whether it conveys the enzymatic activity directly, or merely facilitates the activity of an associated phospholipase. {ECO:0000305|PubMed:21256127}. |
Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
- In-frame and retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
- In-frame and not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for HNRNPM-MCOLN1 |
For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for HNRNPM-MCOLN1 |
Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
Hgene | Hgene's interactors | Tgene | Tgene's interactors |
- Retained PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
- Lost PPIs in in-frame fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
- Retained PPIs, but lost function due to frame-shift fusion. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for HNRNPM-MCOLN1 |
Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for HNRNPM-MCOLN1 |
Diseases associated with fusion partners. (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |