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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:HSPA1B-SLC1A3 (FusionGDB2 ID:37862) |
Fusion Gene Summary for HSPA1B-SLC1A3 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: HSPA1B-SLC1A3 | Fusion gene ID: 37862 | Hgene | Tgene | Gene symbol | HSPA1B | SLC1A3 | Gene ID | 3304 | 6507 |
| Gene name | heat shock protein family A (Hsp70) member 1B | solute carrier family 1 member 3 | |
| Synonyms | HSP70-1|HSP70-1B|HSP70-2|HSP70.1|HSP70.2|HSP72|HSPA1|HSX70 | EA6|EAAT1|GLAST|GLAST1 | |
| Cytomap | 6p21.33 | 5p13.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | heat shock 70 kDa protein 1BHSP70-1/HSP70-2HSP70.1/HSP70.2Heat shock 70 kDa protein 1Heat shock 70 kDa protein 1Aheat shock 70 kDa protein 1/2heat shock 70 kDa protein 1A/1Bheat shock 70 kDa protein 2heat shock 70kD protein 1Bheat shock 70kDa pro | excitatory amino acid transporter 1GLAST-1sodium-dependent glutamate/aspartate transporter 1solute carrier family 1 (glial high affinity glutamate transporter), member 3 | |
| Modification date | 20200313 | 20200320 | |
| UniProtAcc | P0DMV9 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000375650, ENST00000391548, ENST00000391555, ENST00000445736, ENST00000450744, ENST00000545241, | ENST00000265113, ENST00000381918, ENST00000506725, | |
| Fusion gene scores | * DoF score | 4 X 18 X 6=432 | 12 X 11 X 7=924 |
| # samples | 12 | 15 | |
| ** MAII score | log2(12/432*10)=-1.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(15/924*10)=-2.62293035092018 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: HSPA1B [Title/Abstract] AND SLC1A3 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | HSPA1B(31798031)-SLC1A3(36606541), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | HSPA1B | GO:0006402 | mRNA catabolic process | 10205060 |
| Hgene | HSPA1B | GO:0031396 | regulation of protein ubiquitination | 16809764 |
| Hgene | HSPA1B | GO:0031397 | negative regulation of protein ubiquitination | 12150907 |
| Hgene | HSPA1B | GO:0034605 | cellular response to heat | 24061851 |
| Hgene | HSPA1B | GO:0042026 | protein refolding | 15603737|21231916 |
| Hgene | HSPA1B | GO:0046034 | ATP metabolic process | 23921388 |
| Hgene | HSPA1B | GO:0060548 | negative regulation of cell death | 12150907 |
| Hgene | HSPA1B | GO:0090084 | negative regulation of inclusion body assembly | 15603737|21231916 |
| Tgene | SLC1A3 | GO:0015813 | L-glutamate transmembrane transport | 26690923 |
| Tgene | SLC1A3 | GO:0051938 | L-glutamate import | 7521911 |
| Tgene | SLC1A3 | GO:0070779 | D-aspartate import across plasma membrane | 7521911 |
| Tgene | SLC1A3 | GO:0071805 | potassium ion transmembrane transport | 20477940 |
| Tgene | SLC1A3 | GO:0098712 | L-glutamate import across plasma membrane | 26690923 |
| Tgene | SLC1A3 | GO:0140009 | L-aspartate import across plasma membrane | 20477940 |
| Tgene | SLC1A3 | GO:1902476 | chloride transmembrane transport | 20477940 |
| Fusion gene breakpoints across HSPA1B (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across SLC1A3 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | LGG | TCGA-FG-8191-01A | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
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Fusion Gene ORF analysis for HSPA1B-SLC1A3 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-5UTR | ENST00000375650 | ENST00000265113 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| 5CDS-intron | ENST00000375650 | ENST00000381918 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| 5CDS-intron | ENST00000375650 | ENST00000506725 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-5UTR | ENST00000391548 | ENST00000265113 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-5UTR | ENST00000391555 | ENST00000265113 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-5UTR | ENST00000445736 | ENST00000265113 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-5UTR | ENST00000450744 | ENST00000265113 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-5UTR | ENST00000545241 | ENST00000265113 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000391548 | ENST00000381918 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000391548 | ENST00000506725 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000391555 | ENST00000381918 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000391555 | ENST00000506725 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000445736 | ENST00000381918 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000445736 | ENST00000506725 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000450744 | ENST00000381918 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000450744 | ENST00000506725 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000545241 | ENST00000381918 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| intron-intron | ENST00000545241 | ENST00000506725 | HSPA1B | chr6 | 31798031 | - | SLC1A3 | chr5 | 36606541 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for HSPA1B-SLC1A3 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for HSPA1B-SLC1A3 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:31798031/:36606541) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| HSPA1B | . |
| FUNCTION: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The co-chaperones are of three types: J-domain co-chaperones such as HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1 (PubMed:24012426, PubMed:26865365, PubMed:24318877). Maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Its acetylation/deacetylation state determines whether it functions in protein refolding or protein degradation by controlling the competitive binding of co-chaperones HOPX and STUB1. During the early stress response, the acetylated form binds to HOPX which assists in chaperone-mediated protein refolding, thereafter, it is deacetylated and binds to ubiquitin ligase STUB1 that promotes ubiquitin-mediated protein degradation (PubMed:27708256). Regulates centrosome integrity during mitosis, and is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle (PubMed:27137183). Enhances STUB1-mediated SMAD3 ubiquitination and degradation and facilitates STUB1-mediated inhibition of TGF-beta signaling (PubMed:24613385). Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223). {ECO:0000269|PubMed:22528486, ECO:0000269|PubMed:23973223, ECO:0000269|PubMed:24318877, ECO:0000269|PubMed:24613385, ECO:0000269|PubMed:27137183, ECO:0000269|PubMed:27708256, ECO:0000303|PubMed:24012426, ECO:0000303|PubMed:26865365}.; FUNCTION: (Microbial infection) In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. {ECO:0000269|PubMed:16537599}. | FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for HSPA1B-SLC1A3 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for HSPA1B-SLC1A3 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for HSPA1B-SLC1A3 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for HSPA1B-SLC1A3 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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