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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ISCU-CLASP2 (FusionGDB2 ID:40299)

Fusion Gene Summary for ISCU-CLASP2

check button Fusion gene summary
Fusion gene informationFusion gene name: ISCU-CLASP2
Fusion gene ID: 40299
HgeneTgene
Gene symbol

ISCU

CLASP2

Gene ID

23479

23122

Gene nameiron-sulfur cluster assembly enzymecytoplasmic linker associated protein 2
Synonyms2310020H20Rik|HML|ISU2|NIFU|NIFUN|hnifU-
Cytomap

12q23.3

3p22.3

Type of geneprotein-codingprotein-coding
Descriptioniron-sulfur cluster assembly enzyme ISCU, mitochondrialIscU iron-sulfur cluster scaffold homolognifU-like N-terminal domain-containing proteinCLIP-associating protein 2CLIP-associating protein CLASP2multiple asters (Mast)-like homolog 2protein Orbit homolog 2
Modification date2020031320200313
UniProtAcc

Q9H1K1

O75122

Ensembl transtripts involved in fusion geneENST00000311893, ENST00000338291, 
ENST00000392807, ENST00000431221, 
ENST00000535729, ENST00000539593, 
ENST00000540154, ENST00000547005, 
ENST00000307312, ENST00000313350, 
ENST00000333778, ENST00000359576, 
ENST00000399362, ENST00000461133, 
ENST00000468888, ENST00000480013, 
ENST00000482896, ENST00000487200, 
ENST00000539981, 
Fusion gene scores* DoF score2 X 2 X 1=415 X 10 X 9=1350
# samples 215
** MAII scorelog2(2/4*10)=2.32192809488736log2(15/1350*10)=-3.16992500144231
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ISCU [Title/Abstract] AND CLASP2 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointISCU(108957176)-CLASP2(33666703), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across ISCU (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across CLASP2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/ADA571580ISCUchr12

108957176

+CLASP2chr3

33666703

-


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Fusion Gene ORF analysis for ISCU-CLASP2

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
intron-intronENST00000311893ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000311893ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000338291ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000392807ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000431221ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000535729ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000539593ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000540154ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000307312ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000313350ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000333778ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000359576ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000399362ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000461133ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000468888ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000480013ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000482896ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000487200ISCUchr12

108957176

+CLASP2chr3

33666703

-
intron-intronENST00000547005ENST00000539981ISCUchr12

108957176

+CLASP2chr3

33666703

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ISCU-CLASP2


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for ISCU-CLASP2


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:108957176/:33666703)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
ISCU

Q9H1K1

CLASP2

O75122

FUNCTION: Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity). {ECO:0000250|UniProtKB:Q03020, ECO:0000269|PubMed:11060020}.; FUNCTION: [Isoform 2]: Functions as a cytoplasmic scaffold protein for the de novo synthesis of iron-sulfur clusters in the cytoplasm. {ECO:0000269|PubMed:29309586}.FUNCTION: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. {ECO:0000269|PubMed:11290329, ECO:0000269|PubMed:15631994, ECO:0000269|PubMed:16824950, ECO:0000269|PubMed:16866869, ECO:0000269|PubMed:16914514, ECO:0000269|PubMed:17543864, ECO:0000269|PubMed:20937854, ECO:0000269|PubMed:26003921}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ISCU-CLASP2


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ISCU-CLASP2


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ISCU-CLASP2


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ISCU-CLASP2


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource