Home

Download

Statistics

Examples

Help

Contact

	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:ISG15-IFITM1 (FusionGDB2 ID:40301)

Fusion Gene Summary for ISG15-IFITM1

Fusion gene summary

Fusion gene information	Fusion gene name: ISG15-IFITM1
	Fusion gene ID: 40301
		Hgene	Tgene
	Gene symbol	ISG15	IFITM1
	Gene ID	9636	8519
	Gene name	ISG15 ubiquitin like modifier	interferon induced transmembrane protein 1
	Synonyms	G1P2\|IFI15\|IMD38\|IP17\|UCRP\|hUCRP	9-27\|CD225\|DSPA2a\|IFI17\|LEU13
	Cytomap	1p36.33	11p15.5
	Type of gene	protein-coding	protein-coding
	Description	ubiquitin-like protein ISG15interferon, alpha-inducible protein (clone IFI-15K)interferon-induced 17-kDa/15-kDa proteininterferon-stimulated protein, 15 kDaubiquitin cross-reactive protein	interferon-induced transmembrane protein 1dispanin subfamily A member 2ainterferon-induced protein 17interferon-inducible protein 9-27leu-13 antigen
	Modification date	20200313	20200313
	UniProtAcc	P05161	A6NMD0
	Ensembl transtripts involved in fusion gene	ENST00000379389,	ENST00000525554, ENST00000408968, ENST00000528780, ENST00000328221,
Fusion gene scores	* DoF score	2 X 1 X 1=2	7 X 8 X 5=280
	# samples	1	8
	** MAII score	log2(1/2*10)=2.32192809488736	log2(8/280*10)=-1.8073549220576 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: ISG15 [Title/Abstract] AND IFITM1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	ISG15(949543)-IFITM1(314961), # samples:1
Anticipated loss of major functional domain due to fusion event.	ISG15-IFITM1 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ISG15	GO:0007229	integrin-mediated signaling pathway	29100055
Hgene	ISG15	GO:0031397	negative regulation of protein ubiquitination	18305167
Hgene	ISG15	GO:0032020	ISG15-protein conjugation	16122702\|21543490
Hgene	ISG15	GO:0034340	response to type I interferon	22859821
Hgene	ISG15	GO:0072608	interleukin-10 secretion	29100055
Hgene	ISG15	GO:0072643	interferon-gamma secretion	29100055
Tgene	IFITM1	GO:0009615	response to virus	21253575\|22479637
Tgene	IFITM1	GO:0034341	response to interferon-gamma	21253575
Tgene	IFITM1	GO:0035455	response to interferon-alpha	22479637
Tgene	IFITM1	GO:0035456	response to interferon-beta	21253575
Tgene	IFITM1	GO:0045071	negative regulation of viral genome replication	21253575
Tgene	IFITM1	GO:0046597	negative regulation of viral entry into host cell	21253575\|23358889

Fusion gene breakpoints across ISG15 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across IFITM1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	UCEC	TCGA-EY-A1GR-01A	ISG15	chr1	949543	+	IFITM1	chr11	314961	+

Top

Fusion Gene ORF analysis for ISG15-IFITM1

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-intron	ENST00000379389	ENST00000525554	ISG15	chr1	949543	+	IFITM1	chr11	314961	+
Frame-shift	ENST00000379389	ENST00000408968	ISG15	chr1	949543	+	IFITM1	chr11	314961	+
Frame-shift	ENST00000379389	ENST00000528780	ISG15	chr1	949543	+	IFITM1	chr11	314961	+
In-frame	ENST00000379389	ENST00000328221	ISG15	chr1	949543	+	IFITM1	chr11	314961	+

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000379389

ISG15

chr1

949543

ENST00000328221

IFITM1

chr11

314961

810

531

468

148

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000379389	ENST00000328221	ISG15	chr1	949543	+	IFITM1	chr11	314961	+	0.19841786	0.80158216

Top

Fusion Genomic Features for ISG15-IFITM1

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

1-p

p (fusion gene breakpoint)

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

Top

Fusion Protein Features for ISG15-IFITM1

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:949543/chr11:314961)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ISG15 P05161	IFITM1 A6NMD0
FUNCTION: Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade thereby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10; the interaction is mediated by ITGAL (PubMed:29100055). {ECO:0000269\|PubMed:1373138, ECO:0000269\|PubMed:16009940, ECO:0000269\|PubMed:16112642, ECO:0000269\|PubMed:16428300, ECO:0000269\|PubMed:16434471, ECO:0000269\|PubMed:16872604, ECO:0000269\|PubMed:18305167, ECO:0000269\|PubMed:19270716, ECO:0000269\|PubMed:19357168, ECO:0000269\|PubMed:2005397, ECO:0000269\|PubMed:20133869, ECO:0000269\|PubMed:20308324, ECO:0000269\|PubMed:20639253, ECO:0000269\|PubMed:21543490, ECO:0000269\|PubMed:22693631, ECO:0000269\|PubMed:22859821, ECO:0000269\|PubMed:23229543, ECO:0000269\|PubMed:29100055, ECO:0000269\|PubMed:7526157, ECO:0000269\|PubMed:8550581}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000328221

37_57

126.0

Intramembrane

Helical

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000408968

37_57

126.0

Intramembrane

Helical

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000528780

37_57

126.0

Intramembrane

Helical

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000328221

108_125

126.0

Topological domain

Extracellular

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000328221

1_36

126.0

Topological domain

Cytoplasmic

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000328221

58_86

126.0

Topological domain

Cytoplasmic

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000408968

108_125

126.0

Topological domain

Extracellular

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000408968

1_36

126.0

Topological domain

Cytoplasmic

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000408968

58_86

126.0

Topological domain

Cytoplasmic

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000528780

108_125

126.0

Topological domain

Extracellular

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000528780

1_36

126.0

Topological domain

Cytoplasmic

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000528780

58_86

126.0

Topological domain

Cytoplasmic

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000328221

87_107

126.0

Transmembrane

Helical

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000408968

87_107

126.0

Transmembrane

Helical

Tgene

IFITM1

chr1:949543

chr11:314961

ENST00000528780

87_107

126.0

Transmembrane

Helical

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ISG15

chr1:949543

chr11:314961

ENST00000379389

2_78

166.0

Domain

Ubiquitin-like 1

Hgene

ISG15

chr1:949543

chr11:314961

ENST00000379389

79_157

166.0

Domain

Ubiquitin-like 2

Hgene

ISG15

chr1:949543

chr11:314961

ENST00000379389

152_157

166.0

Motif

Note=LRLRGG

Hgene

ISG15

chr1:949543

chr11:314961

ENST00000379389

153_157

166.0

Region

Involved in the ligation of specific target proteins

Top

Fusion Gene Sequence for ISG15-IFITM1

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>40301_40301_1_ISG15-IFITM1_ISG15_chr1_949543_ENST00000379389_IFITM1_chr11_314961_ENST00000328221_length(transcript)=810nt_BP=531nt
CGCAGGCTCGGCGGCACGCCCCCTGACGTGTGTGCCTCAGGCTTATAATAGGGCCGGTGCTGCCTGCCGAAGCCGGCGGCTGAGAGGCAG
CGAACTCATCTTTGCCAGTACAGGAGCTTGTGCCGTGGCCCACAGCCCACAGCCCACAGCCATGAGCCAGGGCCTGGGCCCCGGCAGCAC
GGTCCTGCTGGTGGTGGACAAATGCGACGAACCTCTGAGCATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTACGGCT
GACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGAGGGTGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCC
CCTGGAGGACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTGTTCATGAATCTGCGCCTGCGGGGAGGCGGCACAGA
GCCTGGCGGGCGGAGCTAAGGGCCTCCACCAGCATCCGAGCAGGATCAAGGGCCGGAAATAAAGGCTGTTGTAAAGAGAAAAGGCCTATG
CCTCCACCGCCAAGTGCCTGAACATCTGGGCCCTGATTCTGGGCATCCTCATGACCATTGGATTCATCCTGTTACTGGTATTCGGCTCTG
TGACAGTCTACCATATTATGTTACAGATAATACAGGAAAAACGGGGTTACTAGTAGCCGCCCATAGCCTGCAACCTTTGCACTCCACTGT
GCAATGCTGGCCCTGCACGCTGGGGCTGTTGCCCCTGCCCCCTTGGTCCTGCCCCTAGATACAGCAGTTTATACCCACACACCTGTCTAC

>40301_40301_1_ISG15-IFITM1_ISG15_chr1_949543_ENST00000379389_IFITM1_chr11_314961_ENST00000328221_length(amino acids)=148AA_BP=
MTCVPQAYNRAGAACRSRRLRGSELIFASTGACAVAHSPQPTAMSQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHL

--------------------------------------------------------------

Top

Fusion Gene PPI Analysis for ISG15-IFITM1

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Still interaction with
Tgene	IFITM1	chr1:949543	chr11:314961	ENST00000328221		0	3	84_125	0	126.0	CAV1
Tgene	IFITM1	chr1:949543	chr11:314961	ENST00000408968		0	2	84_125	0	126.0	CAV1
Tgene	IFITM1	chr1:949543	chr11:314961	ENST00000528780		0	3	84_125	0	126.0	CAV1

- Lost PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs for ISG15-IFITM1

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

Top

Related Diseases for ISG15-IFITM1

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source