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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:LOXL3-HTRA2 (FusionGDB2 ID:49460) |
Fusion Gene Summary for LOXL3-HTRA2 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: LOXL3-HTRA2 | Fusion gene ID: 49460 | Hgene | Tgene | Gene symbol | LOXL3 | HTRA2 | Gene ID | 84695 | 27429 |
| Gene name | lysyl oxidase like 3 | HtrA serine peptidase 2 | |
| Synonyms | LOXL | MGCA8|OMI|PARK13|PRSS25 | |
| Cytomap | 2p13.1 | 2p13.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | lysyl oxidase homolog 3lysyl oxidase-like protein 3 | serine protease HTRA2, mitochondrialHtrA-like serine proteaseOmi stress-regulated endoproteaseepididymis secretory sperm binding proteinhigh temperature requirement protein A2protease, serine, 25serine protease 25serine proteinase OMI | |
| Modification date | 20200320 | 20200313 | |
| UniProtAcc | P58215 | O43464 | |
| Ensembl transtripts involved in fusion gene | ENST00000264094, ENST00000393937, ENST00000409249, ENST00000409549, ENST00000409986, ENST00000484369, | ENST00000258080, ENST00000352222, ENST00000467961, | |
| Fusion gene scores | * DoF score | 4 X 4 X 2=32 | 1 X 1 X 1=1 |
| # samples | 4 | 1 | |
| ** MAII score | log2(4/32*10)=0.321928094887362 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(1/1*10)=3.32192809488736 | |
| Context | PubMed: LOXL3 [Title/Abstract] AND HTRA2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | LOXL3(74759746)-HTRA2(74759841), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | LOXL3 | GO:0001837 | epithelial to mesenchymal transition | 16096638 |
| Hgene | LOXL3 | GO:0018057 | peptidyl-lysine oxidation | 28065600 |
| Hgene | LOXL3 | GO:0045892 | negative regulation of transcription, DNA-templated | 16096638 |
| Tgene | HTRA2 | GO:0006508 | proteolysis | 25118933 |
| Tgene | HTRA2 | GO:0010942 | positive regulation of cell death | 11583623 |
| Tgene | HTRA2 | GO:0034605 | cellular response to heat | 10971580 |
| Tgene | HTRA2 | GO:0035458 | cellular response to interferon-beta | 17297443 |
| Tgene | HTRA2 | GO:0043280 | positive regulation of cysteine-type endopeptidase activity involved in apoptotic process | 11583623|11604410|17297443 |
| Tgene | HTRA2 | GO:0044257 | cellular protein catabolic process | 17297443 |
| Tgene | HTRA2 | GO:0071300 | cellular response to retinoic acid | 17297443 |
| Fusion gene breakpoints across LOXL3 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across HTRA2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChiTaRS5.0 | N/A | BM972470 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| ChiTaRS5.0 | N/A | BM987637 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
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Fusion Gene ORF analysis for LOXL3-HTRA2 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| intron-intron | ENST00000264094 | ENST00000258080 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000264094 | ENST00000352222 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000264094 | ENST00000467961 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000393937 | ENST00000258080 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000393937 | ENST00000352222 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000393937 | ENST00000467961 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409249 | ENST00000258080 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409249 | ENST00000352222 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409249 | ENST00000467961 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409549 | ENST00000258080 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409549 | ENST00000352222 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409549 | ENST00000467961 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409986 | ENST00000258080 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409986 | ENST00000352222 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000409986 | ENST00000467961 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000484369 | ENST00000258080 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000484369 | ENST00000352222 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| intron-intron | ENST00000484369 | ENST00000467961 | LOXL3 | chr2 | 74759746 | - | HTRA2 | chr2 | 74759841 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for LOXL3-HTRA2 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for LOXL3-HTRA2 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:74759746/:74759841) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| LOXL3 | HTRA2 |
| FUNCTION: Protein-lysine 6-oxidase that mediates the oxidation of peptidyl lysine residues to allysine in target proteins (PubMed:17018530, PubMed:28065600). Catalyzes the post-translational oxidative deamination of peptidyl lysine residues in precursors of elastin and different types of collagens, a prerequisite in the formation of cross-links between collagens and elastin (PubMed:17018530). Required for somite boundary formation by catalyzing oxidation of fibronectin (FN1), enhancing integrin signaling in myofibers and their adhesion to the myotendinous junction (MTJ) (By similarity). Acts as a regulator of inflammatory response by inhibiting differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): acts by interacting with STAT3 in the nucleus and catalyzing both deacetylation and oxidation of lysine residues on STAT3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600). Also able to catalyze deacetylation of lysine residues on STAT3 (PubMed:28065600). {ECO:0000250|UniProtKB:Q9Z175, ECO:0000269|PubMed:17018530, ECO:0000269|PubMed:28065600}.; FUNCTION: [Isoform 1]: Shows protein-lysine 6-oxidase activity toward elastin and different types of collagens, with the highest activity toward collagen type VIII (PubMed:17018530). {ECO:0000269|PubMed:17018530}.; FUNCTION: [Isoform 2]: Shows protein-lysine 6-oxidase activity toward elastin and different types of collagens, with the highest activity toward collagen type IV (PubMed:17018530). {ECO:0000269|PubMed:17018530}. | FUNCTION: Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. {ECO:0000269|PubMed:15200957, ECO:0000269|PubMed:19502560}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for LOXL3-HTRA2 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for LOXL3-HTRA2 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for LOXL3-HTRA2 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for LOXL3-HTRA2 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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