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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:ANXA4-AGK (FusionGDB2 ID:5054)

Fusion Gene Summary for ANXA4-AGK

check button Fusion gene summary
Fusion gene informationFusion gene name: ANXA4-AGK
Fusion gene ID: 5054
HgeneTgene
Gene symbol

ANXA4

AGK

Gene ID

307

55750

Gene nameannexin A4acylglycerol kinase
SynonymsANX4|HEL-S-274|P32.5|PAP-II|PIG28|PP4-X|ZAP36CATC5|CTRCT38|MTDPS10|MULK
Cytomap

2p13.3

7q34

Type of geneprotein-codingprotein-coding
Descriptionannexin A435-beta calcimedinannexin IV (placental anticoagulant protein II)annexin-4carbohydrate-binding protein p33/p41chromobindin-4endonexin Iepididymis secretory protein Li 274lipocortin IVplacental anticoagulant protein IIproliferation-induacylglycerol kinase, mitochondrialhAGKhsMuLKmulti-substrate lipid kinasemultiple substrate lipid kinase
Modification date2020031320200313
UniProtAcc

P09525

Q53H12

Ensembl transtripts involved in fusion geneENST00000394295, ENST00000536030, 
ENST00000409920, ENST00000418066, 
ENST00000355413, ENST00000473247, 
ENST00000492693, ENST00000495028, 
ENST00000535825, 
Fusion gene scores* DoF score14 X 12 X 7=11767 X 8 X 3=168
# samples 167
** MAII scorelog2(16/1176*10)=-2.877744249949
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(7/168*10)=-1.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: ANXA4 [Title/Abstract] AND AGK [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointANXA4(70052944)-AGK(15936), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneANXA4

GO:0006357

regulation of transcription by RNA polymerase II

20237821

HgeneANXA4

GO:0032088

negative regulation of NF-kappaB transcription factor activity

20237821

TgeneAGK

GO:0045039

protein import into mitochondrial inner membrane

28712724|28712726


check buttonFusion gene breakpoints across ANXA4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across AGK (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/AAV696437ANXA4chr2

70052944

+AGKchr7

15936

+


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Fusion Gene ORF analysis for ANXA4-AGK

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-intronENST00000394295ENST00000355413ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000394295ENST00000473247ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000394295ENST00000492693ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000394295ENST00000495028ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000394295ENST00000535825ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000536030ENST00000355413ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000536030ENST00000473247ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000536030ENST00000492693ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000536030ENST00000495028ANXA4chr2

70052944

+AGKchr7

15936

+
3UTR-intronENST00000536030ENST00000535825ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000409920ENST00000355413ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000409920ENST00000473247ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000409920ENST00000492693ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000409920ENST00000495028ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000409920ENST00000535825ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000418066ENST00000355413ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000418066ENST00000473247ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000418066ENST00000492693ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000418066ENST00000495028ANXA4chr2

70052944

+AGKchr7

15936

+
intron-intronENST00000418066ENST00000535825ANXA4chr2

70052944

+AGKchr7

15936

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for ANXA4-AGK


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for ANXA4-AGK


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:70052944/:15936)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
ANXA4

P09525

AGK

Q53H12

FUNCTION: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. {ECO:0000250}.FUNCTION: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15939762). Does not phosphorylate sphingosine (PubMed:15939762). Phosphorylates ceramide (By similarity). Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol (By similarity). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (PubMed:28712724, PubMed:28712726). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (PubMed:28712724, PubMed:28712726). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (PubMed:28712724). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (PubMed:15939762). {ECO:0000250|UniProtKB:Q9ESW4, ECO:0000269|PubMed:15939762, ECO:0000269|PubMed:28712724, ECO:0000269|PubMed:28712726}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for ANXA4-AGK


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for ANXA4-AGK


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for ANXA4-AGK


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for ANXA4-AGK


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource