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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:MAP3K3-DBF4B (FusionGDB2 ID:51304)

Fusion Gene Summary for MAP3K3-DBF4B

check button Fusion gene summary
Fusion gene informationFusion gene name: MAP3K3-DBF4B
Fusion gene ID: 51304
HgeneTgene
Gene symbol

MAP3K3

DBF4B

Gene ID

4215

80174

Gene namemitogen-activated protein kinase kinase kinase 3DBF4 zinc finger B
SynonymsMAPKKK3|MEKK3ASKL1|CHIFB|DRF1|ZDBF1B
Cytomap

17q23.3

17q21.31|17q21

Type of geneprotein-codingprotein-coding
Descriptionmitogen-activated protein kinase kinase kinase 3MAP/ERK kinase kinase 3MAPK/ERK kinase kinase 3MEK kinase 3MEKK 3protein DBF4 homolog BASK-like protein 1DBF4 homolog BDbf4-related factor 1activator of S-phase kinase-like protein 1chiffon homolog Bzinc finger, DBF-type containing 1B
Modification date2020031320200313
UniProtAcc

Q99759

Q8NFT6

Ensembl transtripts involved in fusion geneENST00000361357, ENST00000361733, 
ENST00000577395, ENST00000579585, 
ENST00000584573, ENST00000577784, 
ENST00000315005, ENST00000398338, 
ENST00000526915, ENST00000393547, 
Fusion gene scores* DoF score10 X 10 X 8=8003 X 3 X 3=27
# samples 134
** MAII scorelog2(13/800*10)=-2.62148837674627
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(4/27*10)=0.567040592723894
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: MAP3K3 [Title/Abstract] AND DBF4B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointMAP3K3(61730016)-DBF4B(42824451), # samples:2
Anticipated loss of major functional domain due to fusion event.MAP3K3-DBF4B seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
MAP3K3-DBF4B seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneMAP3K3

GO:0035556

intracellular signal transduction

15001576

HgeneMAP3K3

GO:0046777

protein autophosphorylation

15001576


check buttonFusion gene breakpoints across MAP3K3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across DBF4B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4ESCATCGA-V5-A7RE-01AMAP3K3chr17

61730016

-DBF4Bchr17

42824451

+
ChimerDB4ESCATCGA-V5-A7RE-01AMAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
ChimerDB4ESCATCGA-V5-A7REMAP3K3chr17

61730016

+DBF4Bchr17

42824450

+


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Fusion Gene ORF analysis for MAP3K3-DBF4B

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000361357ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000361357ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000361357ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000361357ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000361357ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000361357ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000361733ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000361733ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000361733ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000361733ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000361733ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000361733ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000577395ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000577395ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000577395ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000577395ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000577395ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000577395ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000579585ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000579585ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000579585ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000579585ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000579585ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000579585ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000584573ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000584573ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000584573ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000584573ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
5CDS-intronENST00000584573ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
5CDS-intronENST00000584573ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
Frame-shiftENST00000361357ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
Frame-shiftENST00000361357ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
Frame-shiftENST00000361733ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
Frame-shiftENST00000361733ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
Frame-shiftENST00000577395ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
Frame-shiftENST00000577395ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
Frame-shiftENST00000579585ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
Frame-shiftENST00000579585ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
Frame-shiftENST00000584573ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
Frame-shiftENST00000584573ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
intron-3CDSENST00000577784ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
intron-3CDSENST00000577784ENST00000393547MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
intron-intronENST00000577784ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
intron-intronENST00000577784ENST00000315005MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
intron-intronENST00000577784ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
intron-intronENST00000577784ENST00000398338MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+
intron-intronENST00000577784ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824451

+
intron-intronENST00000577784ENST00000526915MAP3K3chr17

61730016

+DBF4Bchr17

42824450

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for MAP3K3-DBF4B


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for MAP3K3-DBF4B


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:61730016/:42824451)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
MAP3K3

Q99759

DBF4B

Q8NFT6

FUNCTION: Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. {ECO:0000269|PubMed:12912994, ECO:0000269|PubMed:14661019, ECO:0000269|PubMed:14743216, ECO:0000269|PubMed:9006902}.FUNCTION: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. {ECO:0000269|PubMed:12065429, ECO:0000269|PubMed:15668232, ECO:0000269|PubMed:17062569}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for MAP3K3-DBF4B


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for MAP3K3-DBF4B


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for MAP3K3-DBF4B


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for MAP3K3-DBF4B


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource