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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:MAP3K5-UBE3D (FusionGDB2 ID:51335) |
Fusion Gene Summary for MAP3K5-UBE3D |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: MAP3K5-UBE3D | Fusion gene ID: 51335 | Hgene | Tgene | Gene symbol | MAP3K5 | UBE3D | Gene ID | 4217 | 90025 |
| Gene name | mitogen-activated protein kinase kinase kinase 5 | ubiquitin protein ligase E3D | |
| Synonyms | ASK1|MAPKKK5|MEKK5 | C6orf157|H10BH|UBE2CBP|YJR141W | |
| Cytomap | 6q23.3 | 6q14.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | mitogen-activated protein kinase kinase kinase 5ASK-1MAP/ERK kinase kinase 5MAPK/ERK kinase kinase 5MEK kinase 5MEKK 5apoptosis signal-regulating kinase 1 | E3 ubiquitin-protein ligase E3DHECT-type E3 ubiquitin transferase E3DUBCH10 binding protein with a hect-like domainubcH10-binding protein with a HECT-like domainubiquitin-conjugating enzyme E2C-binding protein | |
| Modification date | 20200327 | 20200313 | |
| UniProtAcc | Q99683 | . | |
| Ensembl transtripts involved in fusion gene | ENST00000355845, ENST00000359015, ENST00000463140, | ENST00000369745, ENST00000503942, ENST00000369747, | |
| Fusion gene scores | * DoF score | 14 X 11 X 10=1540 | 3 X 3 X 3=27 |
| # samples | 16 | 6 | |
| ** MAII score | log2(16/1540*10)=-3.2667865406949 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(6/27*10)=1.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Context | PubMed: MAP3K5 [Title/Abstract] AND UBE3D [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | MAP3K5(136901438)-UBE3D(83667169), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | MAP3K5-UBE3D seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. MAP3K5-UBE3D seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. MAP3K5-UBE3D seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF. MAP3K5-UBE3D seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | MAP3K5 | GO:0000165 | MAPK cascade | 17210579|21771788 |
| Hgene | MAP3K5 | GO:0000186 | activation of MAPKK activity | 11959862 |
| Hgene | MAP3K5 | GO:0006468 | protein phosphorylation | 11096076|15983381 |
| Hgene | MAP3K5 | GO:0007254 | JNK cascade | 21771788 |
| Hgene | MAP3K5 | GO:0008631 | intrinsic apoptotic signaling pathway in response to oxidative stress | 21771788 |
| Hgene | MAP3K5 | GO:0034198 | cellular response to amino acid starvation | 11096076 |
| Hgene | MAP3K5 | GO:0043065 | positive regulation of apoptotic process | 21771788 |
| Hgene | MAP3K5 | GO:0043280 | positive regulation of cysteine-type endopeptidase activity involved in apoptotic process | 20674765 |
| Hgene | MAP3K5 | GO:0045893 | positive regulation of transcription, DNA-templated | 11096076 |
| Hgene | MAP3K5 | GO:0051403 | stress-activated MAPK cascade | 11096076 |
| Hgene | MAP3K5 | GO:0070301 | cellular response to hydrogen peroxide | 20674765 |
| Tgene | UBE3D | GO:0000209 | protein polyubiquitination | 15749827 |
| Tgene | UBE3D | GO:0006513 | protein monoubiquitination | 15749827 |
| Tgene | UBE3D | GO:0051865 | protein autoubiquitination | 15749827 |
| Fusion gene breakpoints across MAP3K5 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across UBE3D (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | ESCA | TCGA-LN-A49M | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
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Fusion Gene ORF analysis for MAP3K5-UBE3D |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-intron | ENST00000355845 | ENST00000369745 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| 5CDS-intron | ENST00000355845 | ENST00000503942 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| 5CDS-intron | ENST00000359015 | ENST00000369745 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| 5CDS-intron | ENST00000359015 | ENST00000503942 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| Frame-shift | ENST00000359015 | ENST00000369747 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| In-frame | ENST00000355845 | ENST00000369747 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| intron-3CDS | ENST00000463140 | ENST00000369747 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| intron-intron | ENST00000463140 | ENST00000369745 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| intron-intron | ENST00000463140 | ENST00000503942 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000355845 | MAP3K5 | chr6 | 136901438 | - | ENST00000369747 | UBE3D | chr6 | 83667169 | - | 2642 | 1858 | 504 | 1901 | 465 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000355845 | ENST00000369747 | MAP3K5 | chr6 | 136901438 | - | UBE3D | chr6 | 83667169 | - | 0.000487699 | 0.9995123 |
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Fusion Genomic Features for MAP3K5-UBE3D |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
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| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for MAP3K5-UBE3D |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr6:136901438/chr6:83667169) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| MAP3K5 | . |
| FUNCTION: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1). {ECO:0000269|PubMed:10411906, ECO:0000269|PubMed:10688666, ECO:0000269|PubMed:10849426, ECO:0000269|PubMed:11029458, ECO:0000269|PubMed:11154276, ECO:0000269|PubMed:11689443, ECO:0000269|PubMed:11920685, ECO:0000269|PubMed:12697749, ECO:0000269|PubMed:14688258, ECO:0000269|PubMed:14749717, ECO:0000269|PubMed:15023544, ECO:0000269|PubMed:16129676, ECO:0000269|PubMed:17220297, ECO:0000269|PubMed:23102700, ECO:0000269|PubMed:26095851, ECO:0000269|PubMed:8940179, ECO:0000269|PubMed:8974401, ECO:0000269|PubMed:9564042, ECO:0000269|PubMed:9774977}. | FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | MAP3K5 | chr6:136901438 | chr6:83667169 | ENST00000359015 | - | 25 | 30 | 680_938 | 1172 | 1375.0 | Domain | Protein kinase |
| Hgene | MAP3K5 | chr6:136901438 | chr6:83667169 | ENST00000359015 | - | 25 | 30 | 686_694 | 1172 | 1375.0 | Nucleotide binding | ATP |
| Tgene | UBE3D | chr6:136901438 | chr6:83667169 | ENST00000369747 | 7 | 10 | 353_389 | 336 | 390.0 | Region | Note=HECT-like |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | MAP3K5 | chr6:136901438 | chr6:83667169 | ENST00000359015 | - | 25 | 30 | 1245_1285 | 1172 | 1375.0 | Coiled coil | Ontology_term=ECO:0000255 |
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Fusion Gene Sequence for MAP3K5-UBE3D |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >51335_51335_1_MAP3K5-UBE3D_MAP3K5_chr6_136901438_ENST00000355845_UBE3D_chr6_83667169_ENST00000369747_length(transcript)=2642nt_BP=1858nt GGTACCTCAAGTCTATTGTAGAGACAATTTTAATATATAAGCATTTTGTGAAACTGACCACAGAACAGCCTGTGGCCAAGCAAGAACTTG TGGACTTTTGGATGGATTTCCTGGTCGAGGCCACAAAGACAGATGTTACTGTGGTTAGGTTTCCAGTATTAATATTAGAACCAACCAAAA TCTATCAACCTTCTTATTTGTCTATCAACAATGAAGTTGAGGAAAAGACAATCTCTATTTGGCACGTGCTTCCTGATGACAAGAAAGGTA TACATGAGTGGAATTTTAGTGCCTCTTCTGTCAGGGGAGTGAGTATTTCTAAATTTGAAGAAAGATGCTGCTTTCTTTATGTGCTTCACA ATTCTGATGATTTCCAAATCTATTTCTGTACAGAACTTCATTGTAAAAAGTTTTTTGAGATGGTGAACACCATTACCGAAGAGAAGGGGA GAAGCACAGAGGAAGGAGACTGTGAAAGTGACTTGCTGGAGATACTCTCAGCCCCTGCATGAAGAAATAGCATTGCATAAACACCTGAAG CACAAAAATATTGTCCAGTATCTGGGCTCTTTCAGTGAGAATGGTTTCATTAAAATCTTCATGGAGCAGGTCCCTGGAGGAAGTCTTTCT GCTCTCCTTCGTTCCAAATGGGGTCCATTAAAAGACAATGAGCAAACAATTGGCTTTTATACAAAGCAAATACTGGAAGGATTAAAATAT CTCCATGACAATCAGATAGTTCACCGGGACATAAAGGGTGACAATGTGTTGATTAATACCTACAGTGGTGTTCTCAAGATCTCTGACTTC GGAACATCAAAGAGGCTTGCTGGCATAAACCCCTGTACTGAAACTTTTACTGGTACCCTCCAGTATATGGCACCAGAAATAATAGATAAA GGACCAAGAGGCTACGGAAAAGCAGCAGACATCTGGTCTCTGGGCTGTACAATCATTGAAATGGCCACAGGAAAACCCCCATTTTATGAA CTGGGAGAACCACAAGCAGCTATGTTCAAGGTGGGAATGTTTAAAGTCCACCCTGAGATCCCAGAGTCCATGTCTGCAGAGGCCAAGGCA TTCATACTGAAATGTTTTGAACCAGATCCTGACAAGAGAGCCTGTGCTAACGACTTGCTTGTTGATGAGTTTTTAAAAGTTTCAAGCAAA AAGAAAAAGACACAACCTAAGCTTTCAGCTCTTTCAGCTGGATCAAATGAATATCTCAGGAGTATATCCTTGCCGGTACCTGTGCTGGTG GAGGACACCAGCAGCAGCAGTGAGTACGGCTCAGTTTCACCCGACACGGAGTTGAAAGTGGACCCCTTCTCTTTCAAAACAAGAGCCAAG TCCTGCGGAGAAAGAGATGTCAAGGGAATTCGGACACTCTTTTTGGGCATTCCAGATGAGAATTTTGAAGATCACAGTGCTCCTCCTTCC CCTGAAGAAAAAGATTCTGGATTCTTCATGCTGAGGAAGGACAGTGAGAGGCGAGCTACCCTTCACAGGATCCTGACGGAAGACCAAGAC AAAATTGTGAGAAACCTAATGGAATCTTTAGCTCAGGGGGCTGAAGAACCGAAACTAAAATGGGAACACATCACAACCCTCATTGCAAGC CTCAGAGAATTTGTGAGATCCACTGACCGAAAAATCATAGCCACCACACTGTCAAAGCTGAAACTGGAGCTGGACTTCGACAGCCATGGC ATTAGCCAAGTCCAGGTGGTACTCTTTGGTTTTCAAGATGCTGTCAATAAAGTTCTTCGGAATCATAACATCAAGCCGCACTGGATGTTT GCCTTAGACAGTATCATTCGGAAGGCGGTACAGACAGCCATTACCATCCTGGTTCCAGACTTGTCAGCTTGTGGGAAAGTGACATCAGCG TCCACCCGCTAACCCTGCCCTCTGCAACCTGCTTGGAGCTGCTGTTGATATTGTCAAAGAGTAATGCCAATCTGCCTTCATCCCTTCGCC GTGTGAATTCCTTTCAGGTGGCCTTTTTGAAGATGTAACTATTAGAGCTGGGCATTCTCCTCCCCCGGCAGACAGCTCTCAAGCAGGAGC ACACCGAGGCCAGCAGTCAGCTCAGGAGGCATCAATTACAAAGACAAGAACCAGAGCTATGGGATAAGAGCATTGCATTGAAGTTTTAAG TTTACTTGTTTGCATGTATTCTCTAAGAGAAGAGTTAACTTTAGATCTTTGAAAACCCAGGACTGTGAAGAAAGTCATAGTAGCAGGTTG AGGCAAGTGTAGAAGTTACATCTCTATTTCCCACAGAGAGGAATATTCACATATGTGCAGTGTCAGCACCACCTGGAAGGTTATTACCTA ATGGAGATCCATCTGTCCTGTGTGGAATCCAGTGAGGAATTTTATTGGTGGAACTGAATGTTAACTGCTGTAAATCCTAGGAAATAAACT TTTAAAGAGTTTCTCAGGATGATTTAAAACAAATTTTAAAAAAGAAAAGTAGAAAACATTTTATACTTTATTCAGAGGAGATGCTCAATA GATGTTTGTTGAATGAATGATTATCAAATTAAAGGATTTCCTATTTATGCAAATCTGCATGTTTCTTTTGTTATTTCCTTTACCATACAT >51335_51335_1_MAP3K5-UBE3D_MAP3K5_chr6_136901438_ENST00000355845_UBE3D_chr6_83667169_ENST00000369747_length(amino acids)=465AA_BP=449 MHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLI NTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHP EIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTEL KVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPK LKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILV -------------------------------------------------------------- |
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Fusion Gene PPI Analysis for MAP3K5-UBE3D |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| Hgene | MAP3K5 | chr6:136901438 | chr6:83667169 | ENST00000359015 | - | 25 | 30 | 649_1374 | 1172.3333333333333 | 1375.0 | PPIA/CYPA |
| Tgene | UBE3D | chr6:136901438 | chr6:83667169 | ENST00000369747 | 7 | 10 | 235_257 | 336.6666666666667 | 390.0 | UBE2C |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for MAP3K5-UBE3D |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for MAP3K5-UBE3D |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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