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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:MICAL1-AK9 (FusionGDB2 ID:53502) |
Fusion Gene Summary for MICAL1-AK9 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: MICAL1-AK9 | Fusion gene ID: 53502 | Hgene | Tgene | Gene symbol | MICAL1 | AK9 | Gene ID | 64780 | 221264 |
| Gene name | microtubule associated monooxygenase, calponin and LIM domain containing 1 | adenylate kinase 9 | |
| Synonyms | MICAL|MICAL-1|NICAL | AK 9|AKD1|AKD2|C6orf199|C6orf224|dJ70A9.1 | |
| Cytomap | 6q21 | 6q21 | |
| Type of gene | protein-coding | protein-coding | |
| Description | [F-actin]-monooxygenase MICAL1NEDD9-interacting protein with calponin homology and LIM domains[F-actin]-methionine sulfoxide oxidase MICAL1molecule interacting with CasL protein 1protein-methionine sulfoxide oxidase MICAL1 | adenylate kinase 9adenylate kinase domain containing 1adenylate kinase domain containing 2 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q8TDZ2 | Q5TCS8 | |
| Ensembl transtripts involved in fusion gene | ENST00000358577, ENST00000358807, ENST00000368952, ENST00000483856, | ENST00000285397, ENST00000341338, ENST00000355283, ENST00000368948, ENST00000424296, | |
| Fusion gene scores | * DoF score | 1 X 1 X 1=1 | 3 X 3 X 2=18 |
| # samples | 1 | 3 | |
| ** MAII score | log2(1/1*10)=3.32192809488736 | log2(3/18*10)=0.736965594166206 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Context | PubMed: MICAL1 [Title/Abstract] AND AK9 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | MICAL1(109771173)-AK9(109819168), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | MICAL1 | GO:0030042 | actin filament depolymerization | 21864500|28230050 |
| Hgene | MICAL1 | GO:0055114 | oxidation-reduction process | 21864500|26845023 |
| Tgene | AK9 | GO:0006174 | dADP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0006186 | dGDP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0006756 | AMP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0006757 | ATP generation from ADP | 23416111 |
| Tgene | AK9 | GO:0061508 | CDP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0061565 | dAMP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0061566 | CMP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0061567 | dCMP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0061568 | GDP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0061569 | UDP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0061570 | dCDP phosphorylation | 23416111 |
| Tgene | AK9 | GO:0061571 | TDP phosphorylation | 23416111 |
| Fusion gene breakpoints across MICAL1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene breakpoints across AK9 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | BRCA | TCGA-E9-A1RF-01A | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
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Fusion Gene ORF analysis for MICAL1-AK9 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-intron | ENST00000358577 | ENST00000285397 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000358577 | ENST00000341338 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000358577 | ENST00000355283 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000358577 | ENST00000368948 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000358807 | ENST00000285397 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000358807 | ENST00000341338 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000358807 | ENST00000355283 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000358807 | ENST00000368948 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000368952 | ENST00000285397 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000368952 | ENST00000341338 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000368952 | ENST00000355283 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| 5CDS-intron | ENST00000368952 | ENST00000368948 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| Frame-shift | ENST00000358577 | ENST00000424296 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| Frame-shift | ENST00000358807 | ENST00000424296 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| Frame-shift | ENST00000368952 | ENST00000424296 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| intron-3CDS | ENST00000483856 | ENST00000424296 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| intron-intron | ENST00000483856 | ENST00000285397 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| intron-intron | ENST00000483856 | ENST00000341338 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| intron-intron | ENST00000483856 | ENST00000355283 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| intron-intron | ENST00000483856 | ENST00000368948 | MICAL1 | chr6 | 109771173 | - | AK9 | chr6 | 109819168 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
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Fusion Genomic Features for MICAL1-AK9 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for MICAL1-AK9 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:109771173/:109819168) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| MICAL1 | AK9 |
| FUNCTION: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (By similarity). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (PubMed:21864500, PubMed:26845023). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity). May act as Rab effector protein and play a role in vesicle trafficking. {ECO:0000250|UniProtKB:Q8VDP3, ECO:0000269|PubMed:18305261, ECO:0000269|PubMed:21864500, ECO:0000269|PubMed:26845023, ECO:0000269|PubMed:28230050, ECO:0000305, ECO:0000305|PubMed:27552051}. | FUNCTION: Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has both nucleoside monophosphate and diphosphate kinase activities. Catalyzes the phosphorylation of AMP, dAMP, CMP and dCMP with ATP as phosphate donor and of CMP with GTP as phosphate donor. Also catalyzes the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and TTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor. Shows substrate preference of CDP > UDP > ADP > GDP > TDP. {ECO:0000269|PubMed:23416111}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
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Fusion Gene Sequence for MICAL1-AK9 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
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Fusion Gene PPI Analysis for MICAL1-AK9 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for MICAL1-AK9 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for MICAL1-AK9 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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