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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:MICAL2-DKK3 (FusionGDB2 ID:53505)

Fusion Gene Summary for MICAL2-DKK3

check button Fusion gene summary
Fusion gene informationFusion gene name: MICAL2-DKK3
Fusion gene ID: 53505
HgeneTgene
Gene symbol

MICAL2

DKK3

Gene ID

9645

27122

Gene namemicrotubule associated monooxygenase, calponin and LIM domain containing 2dickkopf WNT signaling pathway inhibitor 3
SynonymsMICAL-2|MICAL2PV1|MICAL2PV2REIC|RIG
Cytomap

11p15.3

11p15.3

Type of geneprotein-codingprotein-coding
Description[F-actin]-monooxygenase MICAL2[F-actin]-methionine sulfoxide oxidase MICAL2flavoprotein oxidoreductase MICAL2microtubule associated monoxygenase, calponin and LIM domain containing 2molecule interacting with CasL protein 2protein-methionine sulfoxidedickkopf-related protein 3RIG-like 5-6RIG-like 7-1dickkopf 3 homologdickkopf homolog 3dickkopf-3dkk-3hDkk-3regulated in glioma
Modification date2020032020200313
UniProtAcc

O94851

Q9UBP4

Ensembl transtripts involved in fusion geneENST00000256194, ENST00000342902, 
ENST00000379612, ENST00000527195, 
ENST00000527546, ENST00000537344, 
ENST00000326932, ENST00000396505, 
ENST00000450094, ENST00000525493, 
ENST00000527132, 
Fusion gene scores* DoF score7 X 7 X 5=2458 X 8 X 4=256
# samples 710
** MAII scorelog2(7/245*10)=-1.8073549220576
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(10/256*10)=-1.35614381022528
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: MICAL2 [Title/Abstract] AND DKK3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointMICAL2(12132273)-DKK3(12023984), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneMICAL2

GO:0007010

cytoskeleton organization

24440334

HgeneMICAL2

GO:0030042

actin filament depolymerization

24440334

HgeneMICAL2

GO:0055114

oxidation-reduction process

24440334

TgeneDKK3

GO:0032348

negative regulation of aldosterone biosynthetic process

16981135

TgeneDKK3

GO:0045892

negative regulation of transcription, DNA-templated

16981135

TgeneDKK3

GO:0090090

negative regulation of canonical Wnt signaling pathway

12857724

TgeneDKK3

GO:2000065

negative regulation of cortisol biosynthetic process

16981135


check buttonFusion gene breakpoints across MICAL2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across DKK3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-BR-7715MICAL2chr11

12132273

+DKK3chr11

12023984

-


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Fusion Gene ORF analysis for MICAL2-DKK3

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-intronENST00000256194ENST00000326932MICAL2chr11

12132273

+DKK3chr11

12023984

-
5UTR-intronENST00000256194ENST00000396505MICAL2chr11

12132273

+DKK3chr11

12023984

-
5UTR-intronENST00000256194ENST00000450094MICAL2chr11

12132273

+DKK3chr11

12023984

-
5UTR-intronENST00000256194ENST00000525493MICAL2chr11

12132273

+DKK3chr11

12023984

-
5UTR-intronENST00000256194ENST00000527132MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000342902ENST00000326932MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000342902ENST00000396505MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000342902ENST00000450094MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000342902ENST00000525493MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000342902ENST00000527132MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000379612ENST00000326932MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000379612ENST00000396505MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000379612ENST00000450094MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000379612ENST00000525493MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000379612ENST00000527132MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527195ENST00000326932MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527195ENST00000396505MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527195ENST00000450094MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527195ENST00000525493MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527195ENST00000527132MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527546ENST00000326932MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527546ENST00000396505MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527546ENST00000450094MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527546ENST00000525493MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000527546ENST00000527132MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000537344ENST00000326932MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000537344ENST00000396505MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000537344ENST00000450094MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000537344ENST00000525493MICAL2chr11

12132273

+DKK3chr11

12023984

-
intron-intronENST00000537344ENST00000527132MICAL2chr11

12132273

+DKK3chr11

12023984

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for MICAL2-DKK3


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for MICAL2-DKK3


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:12132273/:12023984)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
MICAL2

O94851

DKK3

Q9UBP4

FUNCTION: Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:24440334). In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (By similarity). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA (PubMed:24440334). {ECO:0000250|UniProtKB:Q8TDZ2, ECO:0000269|PubMed:24440334}.FUNCTION: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for MICAL2-DKK3


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for MICAL2-DKK3


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for MICAL2-DKK3


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for MICAL2-DKK3


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource