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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:APP-TSPEAR (FusionGDB2 ID:5711)

Fusion Gene Summary for APP-TSPEAR

check button Fusion gene summary
Fusion gene informationFusion gene name: APP-TSPEAR
Fusion gene ID: 5711
HgeneTgene
Gene symbol

APP

TSPEAR

Gene ID

351

54084

Gene nameamyloid beta precursor proteinthrombospondin type laminin G domain and EAR repeats
SynonymsAAA|ABETA|ABPP|AD1|APPI|CTFgamma|CVAP|PN-II|PN2|preA4C21orf29|DFNB98|ECTD14|TSP-EAR
Cytomap

21q21.3

21q22.3

Type of geneprotein-codingprotein-coding
Descriptionamyloid-beta precursor proteinalzheimer disease amyloid proteinamyloid beta (A4) precursor proteinamyloid beta A4 proteinamyloid precursor proteinbeta-amyloid peptidebeta-amyloid peptide(1-40)beta-amyloid peptide(1-42)beta-amyloid precursor proteithrombospondin-type laminin G domain and EAR repeat-containing proteinthrombospondin-type laminin G domain and EAR repeats-containing protein
Modification date2020032920200313
UniProtAcc

P05067

P59090

Ensembl transtripts involved in fusion geneENST00000346798, ENST00000348990, 
ENST00000354192, ENST00000357903, 
ENST00000358918, ENST00000359726, 
ENST00000439274, ENST00000440126, 
ENST00000448388, ENST00000474136, 
ENST00000397916, ENST00000323084, 
Fusion gene scores* DoF score31 X 25 X 12=93004 X 3 X 4=48
# samples 335
** MAII scorelog2(33/9300*10)=-4.81669278663694
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(5/48*10)=0.0588936890535686
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context

PubMed: APP [Title/Abstract] AND TSPEAR [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointAPP(27326904)-TSPEAR(45987889), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneAPP

GO:0001934

positive regulation of protein phosphorylation

11404397

HgeneAPP

GO:0008285

negative regulation of cell proliferation

22944668

HgeneAPP

GO:1905606

regulation of presynapse assembly

19726636


check buttonFusion gene breakpoints across APP (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across TSPEAR (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4READTCGA-DC-4745-01AAPPchr21

27326904

-TSPEARchr21

45987889

-
ChimerDB4READTCGA-DC-4745APPchr21

27326904

-TSPEARchr21

45987889

-


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Fusion Gene ORF analysis for APP-TSPEAR

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-5UTRENST00000346798ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000348990ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000354192ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000357903ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000358918ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000359726ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000439274ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000440126ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
5CDS-5UTRENST00000448388ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000346798ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000348990ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000354192ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000357903ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000358918ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000359726ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000439274ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000440126ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
In-frameENST00000448388ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
intron-3CDSENST00000474136ENST00000323084APPchr21

27326904

-TSPEARchr21

45987889

-
intron-5UTRENST00000474136ENST00000397916APPchr21

27326904

-TSPEARchr21

45987889

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000346798APPchr2127326904-ENST00000323084TSPEARchr2145987889-554017213436481204
ENST00000354192APPchr2127326904-ENST00000323084TSPEARchr2145987889-5240142112733481073
ENST00000348990APPchr2127326904-ENST00000323084TSPEARchr2145987889-5428160914735361129
ENST00000357903APPchr2127326904-ENST00000323084TSPEARchr2145987889-5616179716737241185
ENST00000358918APPchr2127326904-ENST00000323084TSPEARchr2145987889-5706188720038141204
ENST00000359726APPchr2127326904-ENST00000323084TSPEARchr2145987889-5538171920036461148
ENST00000448388APPchr2127326904-ENST00000323084TSPEARchr2145987889-5368154916834761102
ENST00000440126APPchr2127326904-ENST00000323084TSPEARchr2145987889-5700188123938081189
ENST00000439274APPchr2127326904-ENST00000323084TSPEARchr2145987889-537215533434801148

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000346798ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0014423640.9985576
ENST00000354192ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0016081210.9983918
ENST00000348990ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.001860090.99813986
ENST00000357903ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0011130340.998887
ENST00000358918ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0018435360.9981565
ENST00000359726ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0026194080.99738055
ENST00000448388ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0019266530.99807334
ENST00000440126ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0009062850.9990938
ENST00000439274ENST00000323084APPchr2127326904-TSPEARchr2145987889-0.0012805250.99871945

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Fusion Genomic Features for APP-TSPEAR


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for APP-TSPEAR


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr21:27326904/chr21:45987889)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
APP

P05067

TSPEAR

P59090

FUNCTION: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis (PubMed:25122912). Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapes in axons (PubMed:17062754, PubMed:23011729). Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. {ECO:0000250, ECO:0000250|UniProtKB:P12023, ECO:0000269|PubMed:17062754, ECO:0000269|PubMed:23011729, ECO:0000269|PubMed:25122912}.; FUNCTION: Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta protein 42 is a more effective reductant than amyloid-beta protein 40. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. APP42-beta may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.; FUNCTION: Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain. {ECO:0000250}.; FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.; FUNCTION: N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318230_260562771.0Compositional biasNote=Asp/Glu-rich (acidic)
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318274_280562771.0Compositional biasNote=Poly-Thr
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116230_260487696.0Compositional biasNote=Asp/Glu-rich (acidic)
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116274_280487696.0Compositional biasNote=Poly-Thr
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015230_260431640.0Compositional biasNote=Asp/Glu-rich (acidic)
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015274_280431640.0Compositional biasNote=Poly-Thr
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217230_260543752.0Compositional biasNote=Asp/Glu-rich (acidic)
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217274_280543752.0Compositional biasNote=Poly-Thr
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317230_260562753.0Compositional biasNote=Asp/Glu-rich (acidic)
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317274_280562753.0Compositional biasNote=Poly-Thr
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217230_260506715.0Compositional biasNote=Asp/Glu-rich (acidic)
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217274_280506715.0Compositional biasNote=Poly-Thr
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217230_260538747.0Compositional biasNote=Asp/Glu-rich (acidic)
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217274_280538747.0Compositional biasNote=Poly-Thr
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-131828_189562771.0DomainE1
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318374_565562771.0DomainE2
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-111628_189487696.0DomainE1
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-101528_189431640.0DomainE1
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-121728_189543752.0DomainE1
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-131728_189562753.0DomainE1
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317374_565562753.0DomainE2
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-121728_189506715.0DomainE1
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-121728_189538747.0DomainE1
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318344_365562771.0MotifOX-2
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116344_365487696.0MotifOX-2
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015344_365431640.0MotifOX-2
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217344_365543752.0MotifOX-2
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317344_365562753.0MotifOX-2
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217344_365506715.0MotifOX-2
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217344_365538747.0MotifOX-2
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318131_189562771.0RegionCuBD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318181_188562771.0RegionNote=Zinc-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-131828_123562771.0RegionGFLD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318391_423562771.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318491_522562771.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318523_540562771.0RegionCollagen-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-131896_110562771.0RegionHeparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116131_189487696.0RegionCuBD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116181_188487696.0RegionNote=Zinc-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-111628_123487696.0RegionGFLD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116391_423487696.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-111696_110487696.0RegionHeparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015131_189431640.0RegionCuBD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015181_188431640.0RegionNote=Zinc-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-101528_123431640.0RegionGFLD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015391_423431640.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-101596_110431640.0RegionHeparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217131_189543752.0RegionCuBD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217181_188543752.0RegionNote=Zinc-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-121728_123543752.0RegionGFLD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217391_423543752.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217491_522543752.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217523_540543752.0RegionCollagen-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-121796_110543752.0RegionHeparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317131_189562753.0RegionCuBD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317181_188562753.0RegionNote=Zinc-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-131728_123562753.0RegionGFLD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317391_423562753.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317491_522562753.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317523_540562753.0RegionCollagen-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-131796_110562753.0RegionHeparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217131_189506715.0RegionCuBD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217181_188506715.0RegionNote=Zinc-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-121728_123506715.0RegionGFLD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217391_423506715.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-121796_110506715.0RegionHeparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217131_189538747.0RegionCuBD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217181_188538747.0RegionNote=Zinc-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-121728_123538747.0RegionGFLD subdomain
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217391_423538747.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217491_522538747.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217523_540538747.0RegionCollagen-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-121796_110538747.0RegionHeparin-binding
TgeneTSPEARchr21:27326904chr21:45987889ENST0000032308401258_27727670.0DomainNote=Laminin G-like
TgeneTSPEARchr21:27326904chr21:45987889ENST00000323084012313_35827670.0RepeatEAR 1
TgeneTSPEARchr21:27326904chr21:45987889ENST00000323084012360_40827670.0RepeatEAR 2
TgeneTSPEARchr21:27326904chr21:45987889ENST00000323084012412_46027670.0RepeatEAR 3
TgeneTSPEARchr21:27326904chr21:45987889ENST00000323084012464_50627670.0RepeatEAR 4
TgeneTSPEARchr21:27326904chr21:45987889ENST00000323084012514_57027670.0RepeatEAR 5
TgeneTSPEARchr21:27326904chr21:45987889ENST00000323084012574_62227670.0RepeatEAR 6
TgeneTSPEARchr21:27326904chr21:45987889ENST00000323084012625_66827670.0RepeatEAR 7

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116374_565487696.0DomainE2
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015374_565431640.0DomainE2
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217374_565543752.0DomainE2
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217374_565506715.0DomainE2
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217374_565538747.0DomainE2
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318724_734562771.0MotifNote=Basolateral sorting signal
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116724_734487696.0MotifNote=Basolateral sorting signal
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015724_734431640.0MotifNote=Basolateral sorting signal
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217724_734543752.0MotifNote=Basolateral sorting signal
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317724_734562753.0MotifNote=Basolateral sorting signal
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217724_734506715.0MotifNote=Basolateral sorting signal
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217724_734538747.0MotifNote=Basolateral sorting signal
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116491_522487696.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116523_540487696.0RegionCollagen-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015491_522431640.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015523_540431640.0RegionCollagen-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217491_522506715.0RegionNote=Heparin-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217523_540506715.0RegionCollagen-binding
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-131818_701562771.0Topological domainExtracellular
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318723_770562771.0Topological domainCytoplasmic
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-111618_701487696.0Topological domainExtracellular
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116723_770487696.0Topological domainCytoplasmic
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-101518_701431640.0Topological domainExtracellular
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015723_770431640.0Topological domainCytoplasmic
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-121718_701543752.0Topological domainExtracellular
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217723_770543752.0Topological domainCytoplasmic
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-131718_701562753.0Topological domainExtracellular
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317723_770562753.0Topological domainCytoplasmic
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-121718_701506715.0Topological domainExtracellular
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217723_770506715.0Topological domainCytoplasmic
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-121718_701538747.0Topological domainExtracellular
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217723_770538747.0Topological domainCytoplasmic
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318702_722562771.0TransmembraneHelical
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116702_722487696.0TransmembraneHelical
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015702_722431640.0TransmembraneHelical
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217702_722543752.0TransmembraneHelical
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317702_722562753.0TransmembraneHelical
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217702_722506715.0TransmembraneHelical
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217702_722538747.0TransmembraneHelical


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Fusion Gene Sequence for APP-TSPEAR


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>5711_5711_1_APP-TSPEAR_APP_chr21_27326904_ENST00000346798_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5540nt_BP=1721nt
CAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGA
GGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAA
TGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGA
ACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCC
CCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGA
GAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGG
CATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGC
TGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGC
AGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGA
GGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGA
GGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATT
CTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTT
ACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCT
CGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGT
CATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGT
GGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCG
CCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCG
CGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCA
GGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCA
GGATGAAGTTGACCTGCGCCCCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTTCAGGTTCA
CGGTGCACGGGGACTCCAGCTCTCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCC
TGAAGAATTTTCCATCGTCGTAACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAG
CGACCTGCTGCTGCTCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGGCAGACCCG
AGTGTCCTTCCGCAGCCCGGCCCTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGA
CTGCGGCCTCCCGGTGGACATAATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAG
GAGAGCCAAAGGCCTGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCCAGCAGGAA
CGCCCCGCTGGCGGTGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATATCCCTATGA
AACCAACATTCGAGTGACGCTGGGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCT
GTATCTGTGTGTTGGCAACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCCACCAA
CTCAGAGACCCTGGGCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCCGCCGTCTA
CAAGTGGACCGAAGAGAAGTTCGTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGAT
CTTCCTGGCAGTGGCTAATTTTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTAC
CCCATATCAGAGCATTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCCAACCACCG
GGAAGGCGACAACCACAACATCGACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTC
CGGCGCCTACGACTGGGAGTTCTTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCA
CTCGCACCTCTACATCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTCTTCCAGAT
CGGGGAGAGGATCTTCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGT
CATCTACGAGCTGAACGTGACCGCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGT
GGGAGAAGATTATTTCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAGGGCTACGA
GGGCTTCGTGGCGGTGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTCATCTACTC
CAGCGCCAAGGAGCCCCTCTCCAGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCCAGGTGGGG
CGGGACCCCAGCACCTCCCCCAACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCAGGGTGCAG
GTGGGGCCATCTGCGGCCCTGGTTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTCTGGTCTTG
CAGGCAGAGGCCCAGAGCCCCTGCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTATACATTTTT
ACCTCAAACAACAGGACCTCGGTTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTGAGCAGGTG
TGGTGCCGTCCCGCCATTGCCGCCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGCACCGGTGC
TCCTCTCTAGGCAGCGGGTGGGCACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCCCTCCCAGC
CTCAGTTTACCCGTAAATGCACTAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGGCCCCACCC
ACAGGGACCTCAACTTCTGGGCCCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACTCAGGCTGC
GTGCCTCCGTCTGCCTGTGTGCTGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTCTCTGCAGC
TGGGAGAGCTGCTTGTCTGTCCGTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCTTAACCCAT
GTAGGTGCCAGAGAAACCACCCCCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACCTGCCACGG
TCTTCCAGGAACGGTGCTCCTGCTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCCGCCAATCA
GAACTGTGGACAAAACTTCCTATTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTAATGAAATG
GAAGCCTCCCCTTGTCTGGCAAATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCAGGTCTCAC
TCCAGCCATCGCTGTGGCCAGTGGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAGCAAGGCTT
TCCCAAGCGTCCTGGGCAGGAGGGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTCATTGGGTC
AGCACCTGTGAAAGGAAGATTAAAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCAGGAAGCTG
CCTTTCCCTTTGCCCTAAGCAGACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCTCATGGAAA
GTGCCAATTTACTGAGCACGAGATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAGTCATGTGA
CCACACCCTCCTCCCTCTGCCCCCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACAGACCTGTC

>5711_5711_1_APP-TSPEAR_APP_chr21_27326904_ENST00000346798_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1204AA_BP=562
MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQ
PVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFR
GVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTT
SIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARD
PVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANER
QQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYER
MNQSLSLLYNVPAVAEEIQDEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRV
PNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADV
PFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQP
PCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQ
NIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVI
YKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANS
HSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTV

--------------------------------------------------------------
>5711_5711_2_APP-TSPEAR_APP_chr21_27326904_ENST00000348990_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5428nt_BP=1609nt
AGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAA
GGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCC
GCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTG
AACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAG
TATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGC
CGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGAC
AAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAG
AGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAA
GAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGT
GAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGT
GATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAG
TCTGTGGAAGAGGTGGTTCGAGTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAAT
GAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCA
GAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCA
GCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTAC
ATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAG
CACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTG
ATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGACCTGCGCCCC
CTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTTCAGGTTCACGGTGCACGGGGACTCCAGCTC
TCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTGAAGAATTTTCCATCGTCGTA
ACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGCGACCTGCTGCTGCTCGGCCTG
CGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGGCAGACCCGAGTGTCCTTCCGCAGCCCGGCC
CTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACTGCGGCCTCCCGGTGGACATA
ATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGAGAGCCAAAGGCCTGTTCATG
GGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCCAGCAGGAACGCCCCGCTGGCGGTGCTGTCC
ATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATATCCCTATGAAACCAACATTCGAGTGACGCTG
GGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGTATCTGTGTGTTGGCAACGAG
TGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCCACCAACTCAGAGACCCTGGGCATTGAG
GTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCCGCCGTCTACAAGTGGACCGAAGAGAAGTTC
GTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCTTCCTGGCAGTGGCTAATTTT
GAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTACCCCATATCAGAGCATTGCCACA
CACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCCAACCACCGGGAAGGCGACAACCACAACATC
GACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCGGCGCCTACGACTGGGAGTTC
TTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACTCGCACCTCTACATCCGACTC
CTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTCTTCCAGATCGGGGAGAGGATCTTCCTCGCT
GTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCATCTACGAGCTGAACGTGACC
GCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGGGAGAAGATTATTTCCTGGTG
GTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAGGGCTACGAGGGCTTCGTGGCGGTGCACAGC
CTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTCATCTACTCCAGCGCCAAGGAGCCCCTCTCC
AGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCCAGGTGGGGCGGGACCCCAGCACCTCCCCCA
ACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCAGGGTGCAGGTGGGGCCATCTGCGGCCCTGG
TTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTCTGGTCTTGCAGGCAGAGGCCCAGAGCCCCT
GCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTATACATTTTTACCTCAAACAACAGGACCTCGG
TTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTGAGCAGGTGTGGTGCCGTCCCGCCATTGCCG
CCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGCACCGGTGCTCCTCTCTAGGCAGCGGGTGGG
CACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCCCTCCCAGCCTCAGTTTACCCGTAAATGCAC
TAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGGCCCCACCCACAGGGACCTCAACTTCTGGGC
CCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACTCAGGCTGCGTGCCTCCGTCTGCCTGTGTGC
TGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTCTCTGCAGCTGGGAGAGCTGCTTGTCTGTCC
GTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCTTAACCCATGTAGGTGCCAGAGAAACCACCC
CCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACCTGCCACGGTCTTCCAGGAACGGTGCTCCTG
CTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCCGCCAATCAGAACTGTGGACAAAACTTCCTA
TTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTAATGAAATGGAAGCCTCCCCTTGTCTGGCAA
ATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCAGGTCTCACTCCAGCCATCGCTGTGGCCAGT
GGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAGCAAGGCTTTCCCAAGCGTCCTGGGCAGGAG
GGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTCATTGGGTCAGCACCTGTGAAAGGAAGATTA
AAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCAGGAAGCTGCCTTTCCCTTTGCCCTAAGCAG
ACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCTCATGGAAAGTGCCAATTTACTGAGCACGAG
ATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAGTCATGTGACCACACCCTCCTCCCTCTGCCC
CCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACAGACCTGTCTCCTGGGTGCATCCTTAGCCTT

>5711_5711_2_APP-TSPEAR_APP_chr21_27326904_ENST00000348990_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1129AA_BP=487
MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQ
PVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFR
GVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTT
SIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHF
QEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQ
IRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQC
DLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFS
LTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLK
YPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKAT
SAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAV
ANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWE
VFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRW

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>5711_5711_3_APP-TSPEAR_APP_chr21_27326904_ENST00000354192_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5240nt_BP=1421nt
GCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTC
GCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCT
GGAGGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCA
GTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAA
ATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAA
CTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGA
CAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAA
AGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGT
AGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGA
AGAGGTGGTTCGAGTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGC
CCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCA
AGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGA
GAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGC
TCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCT
AAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGA
GCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGACCTGCGCCCCCTGGACAT
CCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTTCAGGTTCACGGTGCACGGGGACTCCAGCTCTCAGTAGC
CGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTGAAGAATTTTCCATCGTCGTAACTTTGAG
AGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGCGACCTGCTGCTGCTCGGCCTGCGGTTGTC
ACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGGCAGACCCGAGTGTCCTTCCGCAGCCCGGCCCTGGTGGA
TGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACTGCGGCCTCCCGGTGGACATAATGGCCGA
TGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGAGAGCCAAAGGCCTGTTCATGGGACTGGT
GAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCCAGCAGGAACGCCCCGCTGGCGGTGCTGTCCATCCCACG
GGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATATCCCTATGAAACCAACATTCGAGTGACGCTGGGACCCCA
GCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGTATCTGTGTGTTGGCAACGAGTGGGTCTC
CGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCCACCAACTCAGAGACCCTGGGCATTGAGGTGTTCCG
CATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCCGCCGTCTACAAGTGGACCGAAGAGAAGTTCGTCTCATA
TCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCTTCCTGGCAGTGGCTAATTTTGAACCAGA
TGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTACCCCATATCAGAGCATTGCCACACACAGCGC
CCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCCAACCACCGGGAAGGCGACAACCACAACATCGACAGTGT
CATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCGGCGCCTACGACTGGGAGTTCTTCAGTGT
GGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACTCGCACCTCTACATCCGACTCCTGGGCTC
CTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTCTTCCAGATCGGGGAGAGGATCTTCCTCGCTGTGGCAAA
CAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCATCTACGAGCTGAACGTGACCGCGCAGGC
CTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGGGAGAAGATTATTTCCTGGTGGTGGCCAA
CTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAGGGCTACGAGGGCTTCGTGGCGGTGCACAGCCTCCCCAC
CGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTCATCTACTCCAGCGCCAAGGAGCCCCTCTCCAGGGTCCT
GCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCCAGGTGGGGCGGGACCCCAGCACCTCCCCCAACTGACCC
TGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCAGGGTGCAGGTGGGGCCATCTGCGGCCCTGGTTCGGGCA
GGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTCTGGTCTTGCAGGCAGAGGCCCAGAGCCCCTGCCAGTGC
TCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTATACATTTTTACCTCAAACAACAGGACCTCGGTTCATGTC
CCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTGAGCAGGTGTGGTGCCGTCCCGCCATTGCCGCCACCTAT
TTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGCACCGGTGCTCCTCTCTAGGCAGCGGGTGGGCACAGCCC
CTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCCCTCCCAGCCTCAGTTTACCCGTAAATGCACTAGCGCGA
GGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGGCCCCACCCACAGGGACCTCAACTTCTGGGCCCGGGATT
TTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACTCAGGCTGCGTGCCTCCGTCTGCCTGTGTGCTGGCTGTC
CTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTCTCTGCAGCTGGGAGAGCTGCTTGTCTGTCCGTCACAGG
CAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCTTAACCCATGTAGGTGCCAGAGAAACCACCCCCACTCCA
TGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACCTGCCACGGTCTTCCAGGAACGGTGCTCCTGCTTCTGCA
ACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCCGCCAATCAGAACTGTGGACAAAACTTCCTATTTCCTAC
ACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTAATGAAATGGAAGCCTCCCCTTGTCTGGCAAATAATAAA
CTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCAGGTCTCACTCCAGCCATCGCTGTGGCCAGTGGAGAGCC
ACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAGCAAGGCTTTCCCAAGCGTCCTGGGCAGGAGGGTTGGGC
CCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTCATTGGGTCAGCACCTGTGAAAGGAAGATTAAAAACTCG
ACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCAGGAAGCTGCCTTTCCCTTTGCCCTAAGCAGACAGCTGC
AGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCTCATGGAAAGTGCCAATTTACTGAGCACGAGATAAACAG
GTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAGTCATGTGACCACACCCTCCTCCCTCTGCCCCCTCCAGC
CGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACAGACCTGTCTCCTGGGTGCATCCTTAGCCTTGGCAAAAT

>5711_5711_3_APP-TSPEAR_APP_chr21_27326904_ENST00000354192_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1073AA_BP=431
MLPGLALLLLAAWTARALEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCET
HLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAE
VEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAK
HRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVF
NMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDLRPLDILAEVVPSDGATS
GIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLRED
TAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDAT
PRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVE
EHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKW
SHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTF
NGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSA

--------------------------------------------------------------
>5711_5711_4_APP-TSPEAR_APP_chr21_27326904_ENST00000357903_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5616nt_BP=1797nt
CGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGG
TGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTT
TGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTG
CCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATA
CCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCC
AGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTG
ATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCA
AAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTG
TGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACA
CAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATG
ACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCA
CCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCT
CCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAG
AGTACTGCATGGCCGTGTGTGGCAGCGCCATTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGG
ATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGG
AAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAAC
AGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGG
AGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGG
ACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACC
TCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGACC
TGCGCCCCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTTCAGGTTCACGGTGCACGGGGAC
TCCAGCTCTCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTGAAGAATTTTCCA
TCGTCGTAACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGCGACCTGCTGCTGC
TCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGGCAGACCCGAGTGTCCTTCCGCA
GCCCGGCCCTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACTGCGGCCTCCCGG
TGGACATAATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGAGAGCCAAAGGCC
TGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCCAGCAGGAACGCCCCGCTGGCGG
TGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATATCCCTATGAAACCAACATTCGAG
TGACGCTGGGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGTATCTGTGTGTTG
GCAACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCCACCAACTCAGAGACCCTGG
GCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCCGCCGTCTACAAGTGGACCGAAG
AGAAGTTCGTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCTTCCTGGCAGTGG
CTAATTTTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTACCCCATATCAGAGCA
TTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCCAACCACCGGGAAGGCGACAACC
ACAACATCGACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCGGCGCCTACGACT
GGGAGTTCTTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACTCGCACCTCTACA
TCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTCTTCCAGATCGGGGAGAGGATCT
TCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCATCTACGAGCTGA
ACGTGACCGCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGGGAGAAGATTATT
TCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAGGGCTACGAGGGCTTCGTGGCGG
TGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTCATCTACTCCAGCGCCAAGGAGC
CCCTCTCCAGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCCAGGTGGGGCGGGACCCCAGCAC
CTCCCCCAACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCAGGGTGCAGGTGGGGCCATCTGC
GGCCCTGGTTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTCTGGTCTTGCAGGCAGAGGCCCA
GAGCCCCTGCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTATACATTTTTACCTCAAACAACAG
GACCTCGGTTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTGAGCAGGTGTGGTGCCGTCCCGC
CATTGCCGCCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGCACCGGTGCTCCTCTCTAGGCAG
CGGGTGGGCACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCCCTCCCAGCCTCAGTTTACCCGT
AAATGCACTAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGGCCCCACCCACAGGGACCTCAAC
TTCTGGGCCCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACTCAGGCTGCGTGCCTCCGTCTGC
CTGTGTGCTGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTCTCTGCAGCTGGGAGAGCTGCTT
GTCTGTCCGTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCTTAACCCATGTAGGTGCCAGAGA
AACCACCCCCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACCTGCCACGGTCTTCCAGGAACGG
TGCTCCTGCTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCCGCCAATCAGAACTGTGGACAAA
ACTTCCTATTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTAATGAAATGGAAGCCTCCCCTTG
TCTGGCAAATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCAGGTCTCACTCCAGCCATCGCTG
TGGCCAGTGGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAGCAAGGCTTTCCCAAGCGTCCTG
GGCAGGAGGGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTCATTGGGTCAGCACCTGTGAAAG
GAAGATTAAAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCAGGAAGCTGCCTTTCCCTTTGCC
CTAAGCAGACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCTCATGGAAAGTGCCAATTTACTG
AGCACGAGATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAGTCATGTGACCACACCCTCCTCC
CTCTGCCCCCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACAGACCTGTCTCCTGGGTGCATCC

>5711_5711_4_APP-TSPEAR_APP_chr21_27326904_ENST00000357903_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1185AA_BP=543
MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQ
PVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFR
GVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTT
SIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAIPTTAASTPDAVDKYL
ETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRR
RLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQ
DEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEES
DLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRR
RAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGL
YLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKI
FLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATS
GAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSV
IYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTVGCRDWEAFSTTAGAYLIYS

--------------------------------------------------------------
>5711_5711_5_APP-TSPEAR_APP_chr21_27326904_ENST00000358918_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5706nt_BP=1887nt
GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGG
AGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCG
GTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATG
GTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATT
CAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCA
ATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTC
CCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTT
GCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCT
GCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGG
ATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAG
TGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAG
AAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAAC
AAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGAT
GTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCC
AGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGG
ATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGG
AAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAAC
AGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGG
AGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGG
ACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACC
TCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGACC
TGCGCCCCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTTCAGGTTCACGGTGCACGGGGAC
TCCAGCTCTCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTGAAGAATTTTCCA
TCGTCGTAACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGCGACCTGCTGCTGC
TCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGGCAGACCCGAGTGTCCTTCCGCA
GCCCGGCCCTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACTGCGGCCTCCCGG
TGGACATAATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGAGAGCCAAAGGCC
TGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCCAGCAGGAACGCCCCGCTGGCGG
TGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATATCCCTATGAAACCAACATTCGAG
TGACGCTGGGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGTATCTGTGTGTTG
GCAACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCCACCAACTCAGAGACCCTGG
GCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCCGCCGTCTACAAGTGGACCGAAG
AGAAGTTCGTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCTTCCTGGCAGTGG
CTAATTTTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTACCCCATATCAGAGCA
TTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCCAACCACCGGGAAGGCGACAACC
ACAACATCGACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCGGCGCCTACGACT
GGGAGTTCTTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACTCGCACCTCTACA
TCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTCTTCCAGATCGGGGAGAGGATCT
TCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCATCTACGAGCTGA
ACGTGACCGCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGGGAGAAGATTATT
TCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAGGGCTACGAGGGCTTCGTGGCGG
TGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTCATCTACTCCAGCGCCAAGGAGC
CCCTCTCCAGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCCAGGTGGGGCGGGACCCCAGCAC
CTCCCCCAACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCAGGGTGCAGGTGGGGCCATCTGC
GGCCCTGGTTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTCTGGTCTTGCAGGCAGAGGCCCA
GAGCCCCTGCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTATACATTTTTACCTCAAACAACAG
GACCTCGGTTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTGAGCAGGTGTGGTGCCGTCCCGC
CATTGCCGCCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGCACCGGTGCTCCTCTCTAGGCAG
CGGGTGGGCACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCCCTCCCAGCCTCAGTTTACCCGT
AAATGCACTAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGGCCCCACCCACAGGGACCTCAAC
TTCTGGGCCCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACTCAGGCTGCGTGCCTCCGTCTGC
CTGTGTGCTGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTCTCTGCAGCTGGGAGAGCTGCTT
GTCTGTCCGTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCTTAACCCATGTAGGTGCCAGAGA
AACCACCCCCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACCTGCCACGGTCTTCCAGGAACGG
TGCTCCTGCTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCCGCCAATCAGAACTGTGGACAAA
ACTTCCTATTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTAATGAAATGGAAGCCTCCCCTTG
TCTGGCAAATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCAGGTCTCACTCCAGCCATCGCTG
TGGCCAGTGGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAGCAAGGCTTTCCCAAGCGTCCTG
GGCAGGAGGGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTCATTGGGTCAGCACCTGTGAAAG
GAAGATTAAAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCAGGAAGCTGCCTTTCCCTTTGCC
CTAAGCAGACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCTCATGGAAAGTGCCAATTTACTG
AGCACGAGATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAGTCATGTGACCACACCCTCCTCC
CTCTGCCCCCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACAGACCTGTCTCCTGGGTGCATCC

>5711_5711_5_APP-TSPEAR_APP_chr21_27326904_ENST00000358918_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1204AA_BP=562
MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQ
PVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFR
GVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTT
SIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARD
PVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANER
QQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYER
MNQSLSLLYNVPAVAEEIQDEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRV
PNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADV
PFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQP
PCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQ
NIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVI
YKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANS
HSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTV

--------------------------------------------------------------
>5711_5711_6_APP-TSPEAR_APP_chr21_27326904_ENST00000359726_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5538nt_BP=1719nt
GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGG
AGCGTGCGCGGGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCG
GTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATG
GTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATT
CAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCA
ATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTC
CCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTT
GCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCT
GCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGG
ATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAG
TGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAG
AAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGTGTCCCAAAGTTTAC
TCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCG
AGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCA
TGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGG
AATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCC
GCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCG
CAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGG
TTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGG
ATGAAGTTGACCTGCGCCCCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTTCAGGTTCACG
GTGCACGGGGACTCCAGCTCTCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTG
AAGAATTTTCCATCGTCGTAACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGCG
ACCTGCTGCTGCTCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGGCAGACCCGAG
TGTCCTTCCGCAGCCCGGCCCTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACT
GCGGCCTCCCGGTGGACATAATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGA
GAGCCAAAGGCCTGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCCAGCAGGAACG
CCCCGCTGGCGGTGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATATCCCTATGAAA
CCAACATTCGAGTGACGCTGGGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGT
ATCTGTGTGTTGGCAACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCCACCAACT
CAGAGACCCTGGGCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCCGCCGTCTACA
AGTGGACCGAAGAGAAGTTCGTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCT
TCCTGGCAGTGGCTAATTTTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTACCC
CATATCAGAGCATTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCCAACCACCGGG
AAGGCGACAACCACAACATCGACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCG
GCGCCTACGACTGGGAGTTCTTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACT
CGCACCTCTACATCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTCTTCCAGATCG
GGGAGAGGATCTTCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCA
TCTACGAGCTGAACGTGACCGCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGG
GAGAAGATTATTTCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAGGGCTACGAGG
GCTTCGTGGCGGTGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTCATCTACTCCA
GCGCCAAGGAGCCCCTCTCCAGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCCAGGTGGGGCG
GGACCCCAGCACCTCCCCCAACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCAGGGTGCAGGT
GGGGCCATCTGCGGCCCTGGTTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTCTGGTCTTGCA
GGCAGAGGCCCAGAGCCCCTGCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTATACATTTTTAC
CTCAAACAACAGGACCTCGGTTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTGAGCAGGTGTG
GTGCCGTCCCGCCATTGCCGCCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGCACCGGTGCTC
CTCTCTAGGCAGCGGGTGGGCACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCCCTCCCAGCCT
CAGTTTACCCGTAAATGCACTAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGGCCCCACCCAC
AGGGACCTCAACTTCTGGGCCCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACTCAGGCTGCGT
GCCTCCGTCTGCCTGTGTGCTGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTCTCTGCAGCTG
GGAGAGCTGCTTGTCTGTCCGTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCTTAACCCATGT
AGGTGCCAGAGAAACCACCCCCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACCTGCCACGGTC
TTCCAGGAACGGTGCTCCTGCTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCCGCCAATCAGA
ACTGTGGACAAAACTTCCTATTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTAATGAAATGGA
AGCCTCCCCTTGTCTGGCAAATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCAGGTCTCACTC
CAGCCATCGCTGTGGCCAGTGGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAGCAAGGCTTTC
CCAAGCGTCCTGGGCAGGAGGGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTCATTGGGTCAG
CACCTGTGAAAGGAAGATTAAAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCAGGAAGCTGCC
TTTCCCTTTGCCCTAAGCAGACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCTCATGGAAAGT
GCCAATTTACTGAGCACGAGATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAGTCATGTGACC
ACACCCTCCTCCCTCTGCCCCCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACAGACCTGTCTC

>5711_5711_6_APP-TSPEAR_APP_chr21_27326904_ENST00000359726_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1148AA_BP=506
MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQ
PVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFR
GVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTT
SIATTTTTTTESVEEVVRVSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEA
ERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQ
HTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQL
SVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPA
LVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLS
IPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIE
VFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIAT
HSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRL
LGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLV

--------------------------------------------------------------
>5711_5711_7_APP-TSPEAR_APP_chr21_27326904_ENST00000439274_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5372nt_BP=1553nt
CAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCGATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGA
GGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTG
CAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATT
CTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTT
GCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAA
TGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGT
AGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGA
GGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGA
GGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAA
GTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCAT
GTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAACAGCAGCCAGTACCCCTGATGCCGT
TGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAG
AATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTT
CCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCT
CAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAA
GAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCA
GATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGC
CGAGGAGATTCAGGATGAAGTTGACCTGCGCCCCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGAT
AGTTCAGGTTCACGGTGCACGGGGACTCCAGCTCTCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTG
TGACCTCTTCCCTGAAGAATTTTCCATCGTCGTAACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGT
GGCAGAGGAGAGCGACCTGCTGCTGCTCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGC
CTGGCAGACCCGAGTGTCCTTCCGCAGCCCGGCCCTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTC
CCTCACCACGGACTGCGGCCTCCCGGTGGACATAATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGT
CGGCAGCCGGAGGAGAGCCAAAGGCCTGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTG
TCCCAGCAGGAACGCCCCGCTGGCGGTGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAA
ATATCCCTATGAAACCAACATTCGAGTGACGCTGGGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAG
CCGGAAGGGCCTGTATCTGTGTGTTGGCAACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAA
CTTGTCCACCAACTCAGAGACCCTGGGCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCAC
ATCCGCCGTCTACAAGTGGACCGAAGAGAAGTTCGTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCAT
CGGGAAAAAGATCTTCCTGGCAGTGGCTAATTTTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAA
GCTGAAGTTTACCCCATATCAGAGCATTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGT
GGCCAACCACCGGGAAGGCGACAACCACAACATCGACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGAC
CATCGCCACCTCCGGCGCCTACGACTGGGAGTTCTTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTC
CACCAAGGTGCACTCGCACCTCTACATCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGA
GGTCTTCCAGATCGGGGAGAGGATCTTCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGT
CATCAACTCCGTCATCTACGAGCTGAACGTGACCGCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGA
GTTTTTCTCGGTGGGAGAAGATTATTTCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTG
GCAGGGCTACGAGGGCTTCGTGGCGGTGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTA
CCTCATCTACTCCAGCGCCAAGGAGCCCCTCTCCAGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGT
GGCCAGGTGGGGCGGGACCCCAGCACCTCCCCCAACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCA
GGCAGGGTGCAGGTGGGGCCATCTGCGGCCCTGGTTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGAC
GGTCTGGTCTTGCAGGCAGAGGCCCAGAGCCCCTGCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTT
CTATACATTTTTACCTCAAACAACAGGACCTCGGTTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCG
CCTGAGCAGGTGTGGTGCCGTCCCGCCATTGCCGCCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAA
AAGCACCGGTGCTCCTCTCTAGGCAGCGGGTGGGCACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGC
TTCCCTCCCAGCCTCAGTTTACCCGTAAATGCACTAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCC
ACGGCCCCACCCACAGGGACCTCAACTTCTGGGCCCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGG
GACTCAGGCTGCGTGCCTCCGTCTGCCTGTGTGCTGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCT
TGTCTCTGCAGCTGGGAGAGCTGCTTGTCTGTCCGTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCC
CTCTTAACCCATGTAGGTGCCAGAGAAACCACCCCCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCA
CACCTGCCACGGTCTTCCAGGAACGGTGCTCCTGCTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTT
TTCCGCCAATCAGAACTGTGGACAAAACTTCCTATTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTT
CGTAATGAAATGGAAGCCTCCCCTTGTCTGGCAAATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGG
CTCAGGTCTCACTCCAGCCATCGCTGTGGCCAGTGGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAG
CCAGCAAGGCTTTCCCAAGCGTCCTGGGCAGGAGGGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGG
CCTCATTGGGTCAGCACCTGTGAAAGGAAGATTAAAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCC
TGCAGGAAGCTGCCTTTCCCTTTGCCCTAAGCAGACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTT
ATCTCATGGAAAGTGCCAATTTACTGAGCACGAGATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAA
TCAGTCATGTGACCACACCCTCCTCCCTCTGCCCCCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGG

>5711_5711_7_APP-TSPEAR_APP_chr21_27326904_ENST00000439274_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1148AA_BP=506
MLPGLALLLLAAWTARALEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCET
HLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAE
VEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGG
NRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEA
ERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQ
HTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQL
SVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPA
LVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLS
IPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIE
VFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIAT
HSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRL
LGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLV

--------------------------------------------------------------
>5711_5711_8_APP-TSPEAR_APP_chr21_27326904_ENST00000440126_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5700nt_BP=1881nt
AGAGTTCCGGAGGCTTTGGTTGTCCATTGTTAGCTTAGAAGTTGGCGCAGTGTGCGTGTGATCCACGCCTAAATAGCACAGCCTTGCTGT
GCGTGGTAGAAGTTGGGTTAGTGTTGACATGCTGTTGACTCACCCTCCCGAGGATGGAAGCTCTGGCCTGGGTCAAGTTGTGGTCACTGC
AGTTAACAGTTTGTTGATCTCAGGGAGTATTCCACAGTTGCTGATGTAATTGACAATGATTGGAGCCAGCTCTTCCCCAGATTCAAATGG
ACCAATTAGAGGACTTGTTGGTTCTGTTTATCAACTATGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGATTGCCATGT
TCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGG
AAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACT
GGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCC
TTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGA
CATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTT
GCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACT
ATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGG
ACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCA
CCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCT
GGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACT
GCATGGCCGTGTGTGGCAGCGCCATTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGA
ATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGG
CAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAG
CAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACT
ACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGAC
AGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTG
TGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGACCTGCGCC
CCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTTCAGGTTCACGGTGCACGGGGACTCCAGC
TCTCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTGAAGAATTTTCCATCGTCG
TAACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGCGACCTGCTGCTGCTCGGCC
TGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGGCAGACCCGAGTGTCCTTCCGCAGCCCGG
CCCTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACTGCGGCCTCCCGGTGGACA
TAATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGAGAGCCAAAGGCCTGTTCA
TGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCCAGCAGGAACGCCCCGCTGGCGGTGCTGT
CCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATATCCCTATGAAACCAACATTCGAGTGACGC
TGGGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGTATCTGTGTGTTGGCAACG
AGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCCACCAACTCAGAGACCCTGGGCATTG
AGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCCGCCGTCTACAAGTGGACCGAAGAGAAGT
TCGTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCTTCCTGGCAGTGGCTAATT
TTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTACCCCATATCAGAGCATTGCCA
CACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCCAACCACCGGGAAGGCGACAACCACAACA
TCGACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCGGCGCCTACGACTGGGAGT
TCTTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACTCGCACCTCTACATCCGAC
TCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTCTTCCAGATCGGGGAGAGGATCTTCCTCG
CTGTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCATCTACGAGCTGAACGTGA
CCGCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGGGAGAAGATTATTTCCTGG
TGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAGGGCTACGAGGGCTTCGTGGCGGTGCACA
GCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTCATCTACTCCAGCGCCAAGGAGCCCCTCT
CCAGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCCAGGTGGGGCGGGACCCCAGCACCTCCCC
CAACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCAGGGTGCAGGTGGGGCCATCTGCGGCCCT
GGTTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTCTGGTCTTGCAGGCAGAGGCCCAGAGCCC
CTGCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTATACATTTTTACCTCAAACAACAGGACCTC
GGTTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTGAGCAGGTGTGGTGCCGTCCCGCCATTGC
CGCCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGCACCGGTGCTCCTCTCTAGGCAGCGGGTG
GGCACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCCCTCCCAGCCTCAGTTTACCCGTAAATGC
ACTAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGGCCCCACCCACAGGGACCTCAACTTCTGG
GCCCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACTCAGGCTGCGTGCCTCCGTCTGCCTGTGT
GCTGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTCTCTGCAGCTGGGAGAGCTGCTTGTCTGT
CCGTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCTTAACCCATGTAGGTGCCAGAGAAACCAC
CCCCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACCTGCCACGGTCTTCCAGGAACGGTGCTCC
TGCTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCCGCCAATCAGAACTGTGGACAAAACTTCC
TATTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTAATGAAATGGAAGCCTCCCCTTGTCTGGC
AAATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCAGGTCTCACTCCAGCCATCGCTGTGGCCA
GTGGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAGCAAGGCTTTCCCAAGCGTCCTGGGCAGG
AGGGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTCATTGGGTCAGCACCTGTGAAAGGAAGAT
TAAAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCAGGAAGCTGCCTTTCCCTTTGCCCTAAGC
AGACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCTCATGGAAAGTGCCAATTTACTGAGCACG
AGATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAGTCATGTGACCACACCCTCCTCCCTCTGC
CCCCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACAGACCTGTCTCCTGGGTGCATCCTTAGCC

>5711_5711_8_APP-TSPEAR_APP_chr21_27326904_ENST00000440126_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1189AA_BP=547
MEPALPQIQMDQLEDLLVLFINYVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVV
EANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGI
DKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEAT
ERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAIPTTAASTPDAV
DKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAML
NDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVA
EEIQDEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLLTVV
AEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGARFFV
GSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWFDAS
RKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRHFTI
GKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEANQT
IATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQNDSYV
INSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTVGCRDWEAFSTTAGAY

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>5711_5711_9_APP-TSPEAR_APP_chr21_27326904_ENST00000448388_TSPEAR_chr21_45987889_ENST00000323084_length(transcript)=5368nt_BP=1549nt
GTCGGATGATTCAAGCTCACGGGGACGAGCAGGAGCGCTCTCGACTTTTCTAGAGCCTCAGCGTCCTAGGACTCACCTTTCCCTGATCCT
GCACCGTCCCTCTCCTGGCCCCAGACTCTCCCTCCCACTGTTCACGAAGCCCAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAA
CCCCAGATTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACC
TGCATTGATACCAAGGAAGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCA
GTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAG
TTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCAC
ACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGG
GTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGC
GGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAA
GCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGC
ATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGTTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGAC
AAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATG
TCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAG
GAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAAT
GACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAG
TATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATC
CGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAG
GAGATTCAGGATGAAGTTGACCTGCGCCCCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGCGGGATCAGGATAGTT
CAGGTTCACGGTGCACGGGGACTCCAGCTCTCAGTAGCCGCCCCCCGCACCATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGAC
CTCTTCCCTGAAGAATTTTCCATCGTCGTAACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCA
GAGGAGAGCGACCTGCTGCTGCTCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCCGGCGCCTGG
CAGACCCGAGTGTCCTTCCGCAGCCCGGCCCTGGTGGATGGCCGCTGGCACACACTGGTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTC
ACCACGGACTGCGGCCTCCCGGTGGACATAATGGCCGATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGC
AGCCGGAGGAGAGCCAAAGGCCTGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGTCCC
AGCAGGAACGCCCCGCTGGCGGTGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCAGAAGATAACGAGGTGCTAAAATAT
CCCTATGAAACCAACATTCGAGTGACGCTGGGACCCCAGCCACCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGG
AAGGGCCTGTATCTGTGTGTTGGCAACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTG
TCCACCAACTCAGAGACCCTGGGCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCCAATCGCAAAGCCACATCC
GCCGTCTACAAGTGGACCGAAGAGAAGTTCGTCTCATATCAGAACATCCCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGG
AAAAAGATCTTCCTGGCAGTGGCTAATTTTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTG
AAGTTTACCCCATATCAGAGCATTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTCCTGGCGGTGGCC
AACCACCGGGAAGGCGACAACCACAACATCGACAGTGTCATCTACAAGTGGAACCCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATC
GCCACCTCCGGCGCCTACGACTGGGAGTTCTTCAGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACC
AAGGTGCACTCGCACCTCTACATCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGGGAGGTC
TTCCAGATCGGGGAGAGGATCTTCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAGATGCAAGTCCAGAATGATTCCTATGTCATC
AACTCCGTCATCTACGAGCTGAACGTGACCGCGCAGGCCTTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTT
TTCTCGGTGGGAGAAGATTATTTCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAG
GGCTACGAGGGCTTCGTGGCGGTGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGCACCACGGCTGGTGCCTACCTC
ATCTACTCCAGCGCCAAGGAGCCCCTCTCCAGGGTCCTGCGGCTGAGGACACGCTGAGGCCGCGGCTGCCCGGCAGCAACTGGGGTGGCC
AGGTGGGGCGGGACCCCAGCACCTCCCCCAACTGACCCTGGACGTCCAGGTGGGCCCAGCCTAGGGCATCCCGGGCCTGGGCATCAGGCA
GGGTGCAGGTGGGGCCATCTGCGGCCCTGGTTCGGGCAGGGACATCCATCCACCTCTGAGTCCTTCTGAGCCACAGCCCCAGTGACGGTC
TGGTCTTGCAGGCAGAGGCCCAGAGCCCCTGCCAGTGCTCGCCCATCTGAGTGTCAAGCGGGGATTTCGCTGTCAAGGAACCGTTTCTAT
ACATTTTTACCTCAAACAACAGGACCTCGGTTCATGTCCCTGCGGCTGGCCTCTCGCCAGCCACTCCTGCAGTGTGCGTCCGGCCGCCTG
AGCAGGTGTGGTGCCGTCCCGCCATTGCCGCCACCTATTTATTGTGTTTTATCTGAACGGAAGTGTATTTATACTGCTCTTTACAAAAGC
ACCGGTGCTCCTCTCTAGGCAGCGGGTGGGCACAGCCCCTCACCACGGACACGGACAGAGGACCACCTCCCCATACAGAGAGAGGCTTCC
CTCCCAGCCTCAGTTTACCCGTAAATGCACTAGCGCGAGGTCAGAAATCACAAGCCTGGGCATGGCCTGGGTCGCACCCCCTCCCCACGG
CCCCACCCACAGGGACCTCAACTTCTGGGCCCGGGATTTTTTCCCTATTCAGCAGCATTTTAATCACATGTCCTCCAGCTGACTGGGACT
CAGGCTGCGTGCCTCCGTCTGCCTGTGTGCTGGCTGTCCTAGCTCCCTGGTAACAGCATCCTCTGCTGGCTCTCCTGTGGTCATCTTGTC
TCTGCAGCTGGGAGAGCTGCTTGTCTGTCCGTCACAGGCAGTGAGGATGAGAGGGGGTGATGGGCGCAAGGCCCCAGGGGTCCTCCCTCT
TAACCCATGTAGGTGCCAGAGAAACCACCCCCACTCCATGAGAAGAGGCTCAGACAGCAGGCTCCGGGGCCACGCAGCCCAGACCACACC
TGCCACGGTCTTCCAGGAACGGTGCTCCTGCTTCTGCAACACAAATCGGAACAGGAGCGATCCTCAGAGAGTTTACAATGTGCTTTTTCC
GCCAATCAGAACTGTGGACAAAACTTCCTATTTCCTACACCGCAGCTGTCTGTTCACCTTCAGCTCAGAGAGCACTCCTGACCTTTCGTA
ATGAAATGGAAGCCTCCCCTTGTCTGGCAAATAATAAACTCAGTGTCTACTTTTGCCGGCTTTGGGACCTTCGTGTTTTTCCGGGGCTCA
GGTCTCACTCCAGCCATCGCTGTGGCCAGTGGAGAGCCACTCCCCTCTGGGGGCAGTCGCCGAGAGCCTCCTTCCCCTCCGTCCAGCCAG
CAAGGCTTTCCCAAGCGTCCTGGGCAGGAGGGTTGGGCCCTGCTTTCCACCAAGTCACTCAGAAGGTCCCAATCACACATGATTGGCCTC
ATTGGGTCAGCACCTGTGAAAGGAAGATTAAAAACTCGACACCCCAATTCACTCAACCAAGAAGAAAAAAGCTGAAAGCTGAGTCCTGCA
GGAAGCTGCCTTTCCCTTTGCCCTAAGCAGACAGCTGCAGATGAGAGGGTCAGCATCTCCACTAGTGGCTGCTCTGTGTGCCCCTTATCT
CATGGAAAGTGCCAATTTACTGAGCACGAGATAAACAGGTAACTGACTGTTCCCACCTGCTCCTTTTCTCCTGCAGCATGGAGCAATCAG
TCATGTGACCACACCCTCCTCCCTCTGCCCCCTCCAGCCGGCTTTTCCCCTTGACTGAAGCCTTCAACATCATCTTGGGAGAGAGGCACA

>5711_5711_9_APP-TSPEAR_APP_chr21_27326904_ENST00000448388_TSPEAR_chr21_45987889_ENST00000323084_length(amino acids)=1102AA_BP=460
MLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRC
LVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSD
VWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTP
DAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVE
AMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVP
AVAEEIQDEVDLRPLDILAEVVPSDGATSGIRIVQVHGARGLQLSVAAPRTMSFPASRIFSQCDLFPEEFSIVVTLRVPNLPPKRNEYLL
TVVAEESDLLLLGLRLSPAQLHFLFLREDTAGAWQTRVSFRSPALVDGRWHTLVLAVSAGVFSLTTDCGLPVDIMADVPFPATLSVKGAR
FFVGSRRRAKGLFMGLVRQLVLLPGSDATPRLCPSRNAPLAVLSIPRVLQALTGKPEDNEVLKYPYETNIRVTLGPQPPCTEVEDAQFWF
DASRKGLYLCVGNEWVSVLAAKERLDYVEEHQNLSTNSETLGIEVFRIPQVGLFVATANRKATSAVYKWTEEKFVSYQNIPTHQAQAWRH
FTIGKKIFLAVANFEPDEKGQEFSVIYKWSHRKLKFTPYQSIATHSARDWEAFEVDGEHFLAVANHREGDNHNIDSVIYKWNPATRLFEA
NQTIATSGAYDWEFFSVGPYSFLVVANTFNGTSTKVHSHLYIRLLGSFQLFQSFPTFGAADWEVFQIGERIFLAVANSHSYDVEMQVQND
SYVINSVIYELNVTAQAFVKFQDILTCSALDWEFFSVGEDYFLVVANSFDGRTFSVNSIIYRWQGYEGFVAVHSLPTVGCRDWEAFSTTA

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Fusion Gene PPI Analysis for APP-TSPEAR


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318732_751562.3333333333334771.0G(o)-alpha
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116732_751487.3333333333333696.0G(o)-alpha
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015732_751431.3333333333333640.0G(o)-alpha
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217732_751543.3333333333334752.0G(o)-alpha
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317732_751562.3333333333334753.0G(o)-alpha
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217732_751506.3333333333333715.0G(o)-alpha
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217732_751538.3333333333334747.0G(o)-alpha
HgeneAPPchr21:27326904chr21:45987889ENST00000346798-1318695_722562.3333333333334771.0PSEN1
HgeneAPPchr21:27326904chr21:45987889ENST00000348990-1116695_722487.3333333333333696.0PSEN1
HgeneAPPchr21:27326904chr21:45987889ENST00000354192-1015695_722431.3333333333333640.0PSEN1
HgeneAPPchr21:27326904chr21:45987889ENST00000357903-1217695_722543.3333333333334752.0PSEN1
HgeneAPPchr21:27326904chr21:45987889ENST00000358918-1317695_722562.3333333333334753.0PSEN1
HgeneAPPchr21:27326904chr21:45987889ENST00000359726-1217695_722506.3333333333333715.0PSEN1
HgeneAPPchr21:27326904chr21:45987889ENST00000440126-1217695_722538.3333333333334747.0PSEN1


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for APP-TSPEAR


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for APP-TSPEAR


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource