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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:ARHGAP15-RALB (FusionGDB2 ID:5952)

Fusion Gene Summary for ARHGAP15-RALB

Fusion gene summary

Fusion gene information	Fusion gene name: ARHGAP15-RALB
	Fusion gene ID: 5952
		Hgene	Tgene
	Gene symbol	ARHGAP15	RALB
	Gene ID	55843	5899
	Gene name	Rho GTPase activating protein 15	RAS like proto-oncogene B
	Synonyms	BM046	-
	Cytomap	2q22.2-q22.3	2q14.2
	Type of gene	protein-coding	protein-coding
	Description	rho GTPase-activating protein 15rho-type GTPase-activating protein 15	ras-related protein Ral-BRALB Ras like proto-oncogene BRAS-like protein Bras related GTP binding protein Bv-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
	Modification date	20200313	20200320
	UniProtAcc	Q53QZ3	REPS2
	Ensembl transtripts involved in fusion gene	ENST00000295095, ENST00000409869, ENST00000473426,	ENST00000470417, ENST00000272519, ENST00000474855, ENST00000404963, ENST00000420510,
Fusion gene scores	* DoF score	15 X 13 X 5=975	6 X 6 X 3=108
	# samples	16	6
	** MAII score	log2(16/975*10)=-2.60733031374961 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(6/108*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: ARHGAP15 [Title/Abstract] AND RALB [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	ARHGAP15(144008169)-RALB(121050717), # samples:1
Anticipated loss of major functional domain due to fusion event.	ARHGAP15-RALB seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ARHGAP15	GO:0008360	regulation of cell shape	12650940
Tgene	RALB	GO:0009267	cellular response to starvation	24056301

Fusion gene breakpoints across ARHGAP15 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across RALB (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	Non-Cancer	ERR315488	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+

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Fusion Gene ORF analysis for ARHGAP15-RALB

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-3UTR	ENST00000295095	ENST00000470417	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
Frame-shift	ENST00000295095	ENST00000272519	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
Frame-shift	ENST00000295095	ENST00000474855	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
In-frame	ENST00000295095	ENST00000404963	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
In-frame	ENST00000295095	ENST00000420510	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000409869	ENST00000272519	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000409869	ENST00000404963	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000409869	ENST00000420510	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000409869	ENST00000474855	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000473426	ENST00000272519	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000473426	ENST00000404963	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000473426	ENST00000420510	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3CDS	ENST00000473426	ENST00000474855	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3UTR	ENST00000409869	ENST00000470417	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+
intron-3UTR	ENST00000473426	ENST00000470417	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000295095

ARHGAP15

chr2

144008169

ENST00000420510

RALB

chr2

121050717

1166

641

167

760

197

ENST00000295095

ARHGAP15

chr2

144008169

ENST00000404963

RALB

chr2

121050717

2210

641

167

760

197

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000295095	ENST00000420510	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+	0.006600084	0.9934
ENST00000295095	ENST00000404963	ARHGAP15	chr2	144008169	+	RALB	chr2	121050717	+	0.008294889	0.99170506

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Fusion Genomic Features for ARHGAP15-RALB

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	1-p	p (fusion gene breakpoint)
ARHGAP15	chr2	144008169	+	RALB	chr2	121050716	+	0.000221771	0.9997782
ARHGAP15	chr2	144008169	+	RALB	chr2	121050716	+	0.000221771	0.9997782

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for ARHGAP15-RALB

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:144008169/chr2:121050717)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ARHGAP15 Q53QZ3	RALB REPS2
FUNCTION: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction. {ECO:0000269\|PubMed:12650940}.	660

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ARHGAP15

chr2:144008169

chr2:121050717

ENST00000295095

281_470

158

476.0

Domain

Rho-GAP

Hgene

ARHGAP15

chr2:144008169

chr2:121050717

ENST00000295095

79_189

158

476.0

Domain

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000272519

43_51

167

207.0

Motif

Note=Effector region

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000420510

43_51

167

207.0

Motif

Note=Effector region

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000272519

128_131

167

207.0

Nucleotide binding

Note=GTP

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000272519

158_160

167

207.0

Nucleotide binding

Note=GTP

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000272519

21_29

167

207.0

Nucleotide binding

Note=GTP

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000272519

68_72

167

207.0

Nucleotide binding

Note=GTP

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000420510

128_131

167

207.0

Nucleotide binding

Note=GTP

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000420510

158_160

167

207.0

Nucleotide binding

Note=GTP

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000420510

21_29

167

207.0

Nucleotide binding

Note=GTP

Tgene

RALB

chr2:144008169

chr2:121050717

ENST00000420510

68_72

167

207.0

Nucleotide binding

Note=GTP

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Fusion Gene Sequence for ARHGAP15-RALB

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>5952_5952_1_ARHGAP15-RALB_ARHGAP15_chr2_144008169_ENST00000295095_RALB_chr2_121050717_ENST00000404963_length(transcript)=2210nt_BP=641nt
GCCTTTTTCTTCCCCCAGTACTGAAATTAAGCAGCATCCAACACAGGCCTACTCTTACGACATGTGACTTTACTGTTTTCCGTTTTTGTT
GAAAGAGTCATTAACAGTTAGGAGTTGATGGCAGTTTCAATAACAGGTCATTGCCGAGAAAAGGATAGCACTATAATATGCAGAAATCTA
CAAATTCTGATACTTCCGTGGAAACACTGAATTCTACCCGCCAAGGCACAGGAGCTGTGCAAATGAGAATCAAAAATGCCAACAGCCACC
ATGACAGGCTCAGCCAAAGTAAATCCATGATCCTCACCGATGTCGGGAAGGTCACTGAACCTATATCCAGACACAGAAGGAATCATTCAC
AGCATATCTTGAAAGATGTCATTCCTCCATTGGAACAACTGATGGTTGAAAAAGAAGGTTATCTGCAAAAAGCTAAAATTGCAGATGGAG
GAAAGAAACTAAGGAAAAACTGGTCTACTTCCTGGATTGTTCTTTCTAGTCGAAGAATTGAATTTTACAAAGAATCCAAGCAACAGGCTC
TGTCCAATATGAAAACTGGGCACAAACCAGAAAGTGTGGATTTGTGTGGAGCACACATTGAATGGGCCAAGGAAAAATCGAGCAGAAAGA
ATGTCTTTCAGGTGTTCTTTGACCTAATGAGAGAAATCAGAACAAAGAAGATGTCAGAAAACAAAGACAAGAATGGCAAGAAAAGCAGCA
AGAACAAGAAAAGTTTTAAAGAAAGATGTTGCTTACTATGAGTGTCAAGGTGACGGATGAAGCCAGCTGCTCCTAAGGACACAGGGCTGG
GTTGGTAAAGAGAAGGCTATGGTTGACTTCTTGCTTGTGCTTCCCACTCTCCCCGACTTCATTCACTCAAACTTCTTTAAATGGGGAAAA
ATATTTGTGACTCTGTGGCTGGCAGAAGAAATAAGCCCATGCAAGTGGAAGGGCTGCTTTGTCAGGAGGTTGTGGAATTTCTTTCTTCTC
CCCTTCTTCCCTCCCAAAAGCTTAGCTATGTATAAAGTGCCACAGATAGGAAACAGCTGTTAATTACAAAGAGAAAGAATTGTCATAGCA
TCTTATTTTGTTCCTAGTTTTATAACATTACCATCCTTCGTTTTGAACTACAGATGTTGTAGTGGGTTTTGGAGGAGGGAGTGGAGTAAG
ATGCCCTCCCACTTTTATCAGTTTAGTAGTAGTACTGAGAAAAATCCCTTCAGCTCTAAGAACACTGAAAAATCCACCGATTTTTTGGGT
AAGCTTCTTGGCAATACCCTGTGGATCTGAAACAGCTAAAAAAATGAATTTGAATTGCGCCAGATAGGTCAATACCAAGCTTCTGATTCC
TCCTCACATATGAAAAGTGAAAGTTGTGAGTTGTTTTCCTCTTATTTAAACATTGGCCTATTATAATCTGTGTTGGTTATTTTTCTCCTG
TAAGCATCCTGATTTTTCTGTAGGAACTTTTCTTTGGCAGACCAAGTGAAGACTCAGGAATGGTGTGCATTATAAATGACACACATTGCC
ACTTGTGTAGATATTTTTAAGTTCTTTGGCTAAGTCCTCTCCTAACTGCCTGTCCTCTGGTTAGGCCCCTCCCTCTCCACTAGTGGTGAA
TGCATGTGTCTGTCTGATCAGCATCACTGCACACGGAGGTCTAGTGAGCCTCTTGCTAAGTGTCACACACACTCTTCCCAAAGACGTGAT
GAGTTAAAGTTGTATTCTGAAATCATGAAGCCAGAGCCTGTGCCAGACCTTCTGCTACCTCTCATAGAATTGCTCTGTAATTCTAAATTT
AAAATTAGAAGTAGAGAGAGATAAGCCATCGCCCCTTTGCCTCTGAGAATTGGCTGCTGTTTCTAATATAATTATTTTCTAAGATAGCCA
GATAGTTAGAAAAAGATTTTCATTGATGACATATCTTTAAACTTTCTTGCATCAGTATTCTAAATTGAGCAAACTGAAAGATTTTCATCA
GGAAAGGAGCACTGTGGGAAGAGCCCAGTATTCACATTTTTTCCCCATTTTTCAGAAGCGACATTTCATATATAGGTGCCAAAAGTGAAT
CGGGGTGCGGAGAGTGGGAACCTTTTGAATTTATGATTGTCACAGAGATGGTAGAAATTATGATCTGACTGGAAAACAATCCTGTATCCC

>5952_5952_1_ARHGAP15-RALB_ARHGAP15_chr2_144008169_ENST00000295095_RALB_chr2_121050717_ENST00000404963_length(amino acids)=197AA_BP=157
MQKSTNSDTSVETLNSTRQGTGAVQMRIKNANSHHDRLSQSKSMILTDVGKVTEPISRHRRNHSQHILKDVIPPLEQLMVEKEGYLQKAK
IADGGKKLRKNWSTSWIVLSSRRIEFYKESKQQALSNMKTGHKPESVDLCGAHIEWAKEKSSRKNVFQVFFDLMREIRTKKMSENKDKNG

--------------------------------------------------------------
>5952_5952_2_ARHGAP15-RALB_ARHGAP15_chr2_144008169_ENST00000295095_RALB_chr2_121050717_ENST00000420510_length(transcript)=1166nt_BP=641nt
GCCTTTTTCTTCCCCCAGTACTGAAATTAAGCAGCATCCAACACAGGCCTACTCTTACGACATGTGACTTTACTGTTTTCCGTTTTTGTT
GAAAGAGTCATTAACAGTTAGGAGTTGATGGCAGTTTCAATAACAGGTCATTGCCGAGAAAAGGATAGCACTATAATATGCAGAAATCTA
CAAATTCTGATACTTCCGTGGAAACACTGAATTCTACCCGCCAAGGCACAGGAGCTGTGCAAATGAGAATCAAAAATGCCAACAGCCACC
ATGACAGGCTCAGCCAAAGTAAATCCATGATCCTCACCGATGTCGGGAAGGTCACTGAACCTATATCCAGACACAGAAGGAATCATTCAC
AGCATATCTTGAAAGATGTCATTCCTCCATTGGAACAACTGATGGTTGAAAAAGAAGGTTATCTGCAAAAAGCTAAAATTGCAGATGGAG
GAAAGAAACTAAGGAAAAACTGGTCTACTTCCTGGATTGTTCTTTCTAGTCGAAGAATTGAATTTTACAAAGAATCCAAGCAACAGGCTC
TGTCCAATATGAAAACTGGGCACAAACCAGAAAGTGTGGATTTGTGTGGAGCACACATTGAATGGGCCAAGGAAAAATCGAGCAGAAAGA
ATGTCTTTCAGGTGTTCTTTGACCTAATGAGAGAAATCAGAACAAAGAAGATGTCAGAAAACAAAGACAAGAATGGCAAGAAAAGCAGCA
AGAACAAGAAAAGTTTTAAAGAAAGATGTTGCTTACTATGAGTGTCAAGGTGACGGATGAAGCCAGCTGCTCCTAAGGACACAGGGCTGG
GTTGGTAAAGAGAAGGCTATGGTTGACTTCTTGCTTGTGCTTCCCACTCTCCCCGACTTCATTCACTCAAACTTCTTTAAATGGGGAAAA
ATATTTGTGACTCTGTGGCTGGCAGAAGAAATAAGCCCATGCAAGTGGAAGGGCTGCTTTGTCAGGAGGTTGTGGAATTTCTTTCTTCTC
CCCTTCTTCCCTCCCAAAAGCTTAGCTATGTATAAAGTGCCACAGATAGGAAACAGCTGTTAATTACAAAGAGAAAGAATTGTCATAGCA

>5952_5952_2_ARHGAP15-RALB_ARHGAP15_chr2_144008169_ENST00000295095_RALB_chr2_121050717_ENST00000420510_length(amino acids)=197AA_BP=157
MQKSTNSDTSVETLNSTRQGTGAVQMRIKNANSHHDRLSQSKSMILTDVGKVTEPISRHRRNHSQHILKDVIPPLEQLMVEKEGYLQKAK
IADGGKKLRKNWSTSWIVLSSRRIEFYKESKQQALSNMKTGHKPESVDLCGAHIEWAKEKSSRKNVFQVFFDLMREIRTKKMSENKDKNG

--------------------------------------------------------------

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Fusion Gene PPI Analysis for ARHGAP15-RALB

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs for ARHGAP15-RALB

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

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Related Diseases for ARHGAP15-RALB

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source