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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:NPC1-FGD4 (FusionGDB2 ID:59853)

Fusion Gene Summary for NPC1-FGD4

check button Fusion gene summary
Fusion gene informationFusion gene name: NPC1-FGD4
Fusion gene ID: 59853
HgeneTgene
Gene symbol

NPC1

FGD4

Gene ID

4864

121512

Gene nameNPC intracellular cholesterol transporter 1FYVE, RhoGEF and PH domain containing 4
SynonymsNPC|POGZ|SLC65A1CMT4H|FRABP|ZFYVE6
Cytomap

18q11.2

12p11.21

Type of geneprotein-codingprotein-coding
DescriptionNPC intracellular cholesterol transporter 1Niemann-Pick C1 proteintruncated Niemann-Pick C1FYVE, RhoGEF and PH domain-containing protein 4FGD1 family, member 4FGD1-related F-actin-binding proteinactin-filament binding protein frabinzinc finger FYVE domain-containing protein 6
Modification date2020031520200328
UniProtAcc.

Q96M96

Ensembl transtripts involved in fusion geneENST00000269228, ENST00000412552, 
ENST00000540608, 		ENST00000266482, 
ENST00000525053, ENST00000546442, 
ENST00000381025, ENST00000472289, 
ENST00000473513, ENST00000427716, 
ENST00000531134, ENST00000534526, 
Fusion gene scores* DoF score7 X 10 X 8=5609 X 11 X 6=594
# samples 1212
** MAII scorelog2(12/560*10)=-2.22239242133645
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(12/594*10)=-2.30742852519225
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: NPC1 [Title/Abstract] AND FGD4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointNPC1(21124924)-FGD4(32751431), # samples:3
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneNPC1

GO:0006486

protein glycosylation

10821832

HgeneNPC1

GO:0030301

cholesterol transport

18772377

HgeneNPC1

GO:0033344

cholesterol efflux

16141411

HgeneNPC1

GO:0042632

cholesterol homeostasis

12719428

HgeneNPC1

GO:0090150

establishment of protein localization to membrane

23360953


check buttonFusion gene breakpoints across NPC1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across FGD4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUADTCGA-05-4395-01ANPC1chr18

21124924

-FGD4chr12

32751431

+


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Fusion Gene ORF analysis for NPC1-FGD4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-5UTRENST00000269228ENST00000266482NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-5UTRENST00000269228ENST00000525053NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-5UTRENST00000269228ENST00000546442NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-5UTRENST00000412552ENST00000266482NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-5UTRENST00000412552ENST00000525053NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-5UTRENST00000412552ENST00000546442NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-intronENST00000269228ENST00000381025NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-intronENST00000269228ENST00000472289NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-intronENST00000269228ENST00000473513NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-intronENST00000412552ENST00000381025NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-intronENST00000412552ENST00000472289NPC1chr18

21124924

-FGD4chr12

32751431

+
5CDS-intronENST00000412552ENST00000473513NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-3CDSENST00000540608ENST00000427716NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-3CDSENST00000540608ENST00000531134NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-3CDSENST00000540608ENST00000534526NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-5UTRENST00000540608ENST00000266482NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-5UTRENST00000540608ENST00000525053NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-5UTRENST00000540608ENST00000546442NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-intronENST00000540608ENST00000381025NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-intronENST00000540608ENST00000472289NPC1chr18

21124924

-FGD4chr12

32751431

+
5UTR-intronENST00000540608ENST00000473513NPC1chr18

21124924

-FGD4chr12

32751431

+
In-frameENST00000269228ENST00000427716NPC1chr18

21124924

-FGD4chr12

32751431

+
In-frameENST00000269228ENST00000531134NPC1chr18

21124924

-FGD4chr12

32751431

+
In-frameENST00000269228ENST00000534526NPC1chr18

21124924

-FGD4chr12

32751431

+
In-frameENST00000412552ENST00000427716NPC1chr18

21124924

-FGD4chr12

32751431

+
In-frameENST00000412552ENST00000531134NPC1chr18

21124924

-FGD4chr12

32751431

+
In-frameENST00000412552ENST00000534526NPC1chr18

21124924

-FGD4chr12

32751431

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000269228NPC1chr1821124924-ENST00000534526FGD4chr1232751431+420325021842021394
ENST00000269228NPC1chr1821124924-ENST00000531134FGD4chr1232751431+440925021842021394
ENST00000269228NPC1chr1821124924-ENST00000427716FGD4chr1232751431+972025021842021394
ENST00000412552NPC1chr1821124924-ENST00000534526FGD4chr1232751431+27261025232725901
ENST00000412552NPC1chr1821124924-ENST00000531134FGD4chr1232751431+29321025232725900
ENST00000412552NPC1chr1821124924-ENST00000427716FGD4chr1232751431+82431025232725900

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000269228ENST00000534526NPC1chr1821124924-FGD4chr1232751431+0.0009506250.99904937
ENST00000269228ENST00000531134NPC1chr1821124924-FGD4chr1232751431+0.0008202170.9991798
ENST00000269228ENST00000427716NPC1chr1821124924-FGD4chr1232751431+0.0002869090.9997131
ENST00000412552ENST00000534526NPC1chr1821124924-FGD4chr1232751431+0.0007728140.99922717
ENST00000412552ENST00000531134NPC1chr1821124924-FGD4chr1232751431+0.0006041880.99939585
ENST00000412552ENST00000427716NPC1chr1821124924-FGD4chr1232751431+0.0001287330.99987125

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Fusion Genomic Features for NPC1-FGD4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
NPC1chr1821124923-FGD4chr1232751430+0.0001025250.9998975
NPC1chr1821124923-FGD4chr1232751430+0.0001025250.9998975

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for NPC1-FGD4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr18:21124924/chr12:32751431)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.FGD4

Q96M96

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8 (By similarity). {ECO:0000250, ECO:0000269|PubMed:15133042}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225249_2596491279.0Compositional biasNote=Poly-Pro
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225175_2056491279.0RegionNote=Important for cholesterol binding and cholesterol transfer from NPC1 to liposomes
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-122523_2616491279.0Topological domainLumenal
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225283_3506491279.0Topological domainCytoplasmic
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225372_6206491279.0Topological domainLumenal
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225262_2826491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225351_3716491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225621_6416491279.0TransmembraneHelical
TgeneFGD4chr18:21124924chr12:32751431ENST0000038102509206_3930281.0DomainDH
TgeneFGD4chr18:21124924chr12:32751431ENST0000038102509422_5210281.0DomainPH 1
TgeneFGD4chr18:21124924chr12:32751431ENST0000038102509643_7400281.0DomainPH 2
TgeneFGD4chr18:21124924chr12:32751431ENST00000427716317206_393200767.0DomainDH
TgeneFGD4chr18:21124924chr12:32751431ENST00000427716317422_521200767.0DomainPH 1
TgeneFGD4chr18:21124924chr12:32751431ENST00000427716317643_740200767.0DomainPH 2
TgeneFGD4chr18:21124924chr12:32751431ENST00000381025091_1500281.0RegionActin filament-binding
TgeneFGD4chr18:21124924chr12:32751431ENST0000038102509559_6190281.0Zinc fingerFYVE-type
TgeneFGD4chr18:21124924chr12:32751431ENST00000427716317559_619200767.0Zinc fingerFYVE-type

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225620_7856491279.0DomainSSD
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251119_11246491279.0Topological domainCytoplasmic
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251146_11506491279.0Topological domainLumenal
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251172_11946491279.0Topological domainCytoplasmic
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251216_12236491279.0Topological domainLumenal
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251245_12786491279.0Topological domainCytoplasmic
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225642_6536491279.0Topological domainCytoplasmic
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225676_6856491279.0Topological domainLumenal
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225707_7306491279.0Topological domainCytoplasmic
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225752_7596491279.0Topological domainLumenal
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225784_8326491279.0Topological domainCytoplasmic
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225854_10976491279.0Topological domainLumenal
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251098_11186491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251125_11456491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251151_11716491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251195_12156491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-12251224_12446491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225654_6756491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225686_7066491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225731_7516491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225760_7836491279.0TransmembraneHelical
HgeneNPC1chr18:21124924chr12:32751431ENST00000269228-1225833_8536491279.0TransmembraneHelical
TgeneFGD4chr18:21124924chr12:32751431ENST000004277163171_150200767.0RegionActin filament-binding


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Fusion Gene Sequence for NPC1-FGD4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>59853_59853_1_NPC1-FGD4_NPC1_chr18_21124924_ENST00000269228_FGD4_chr12_32751431_ENST00000427716_length(transcript)=9720nt_BP=2502nt
CAGATTCCTTTCAGGTGACTGGGTTGGGAGGAGAAGTCGCTCAGCGTAATTGTCGGAGCCGAAATAAACAGAGACTCCGCGGCCGGGCGG
GACCTTACCCGGAAGTCCCCCAGAACAGCACTACCCTTCGCTGAAACCTCGCCCGCCCCCTCCAACCCAGAGACGAAAAGGGAAAGCATC
ACGGCCAGAAACCGTTGGCACAACTCCACAGATACTCTCCCGGGCCGAGCCAGACTCCATAAGTCCCGCGCCTGGCCCCCGGGGATTGCA
GGGGCTGAGGAGAAGGGCAACACGGGGACCTTGAAGCGGGGTCGCGGCGGCGCCCCAGCCCGGGCCAGGGAGTCCCGGCAGCGGCACCTC
CCAGAAAGGGCGGAGCCGACGACGCCTTCTTCCTTCCTGACCGGCGCGCGCAGCCTGCTGCCGCGGTCAGCGCCTGCTCCTGCTCCTCCG
CTCCTCCTGCGCGGGGTGCTGAAACAGCCCGGGGAAGTAGAGCCGCCTCCGGGGAGCCCAACCAGCCGAACGCCGCCGGCGTCAGCAGCC
TTGCGCGGCCACAGCATGACCGCTCGCGGCCTGGCCCTTGGCCTCCTCCTGCTGCTACTGTGTCCAGCGCAGGTGTTTTCACAGTCCTGT
GTTTGGTATGGAGAGTGTGGAATTGCATATGGGGACAAGAGGTACAATTGCGAATATTCTGGCCCACCAAAACCATTGCCAAAGGATGGA
TATGACTTAGTGCAGGAACTCTGTCCAGGATTCTTCTTTGGCAATGTCAGTCTCTGTTGTGATGTTCGGCAGCTTCAGACACTAAAAGAC
AACCTGCAGCTGCCTCTACAGTTTCTGTCCAGATGTCCATCCTGTTTTTATAACCTACTGAACCTGTTTTGTGAGCTGACATGTAGCCCT
CGACAGAGTCAGTTTTTGAATGTTACAGCTACTGAAGATTATGTTGATCCTGTTACAAACCAGACGAAAACAAATGTGAAAGAGTTACAA
TACTACGTCGGACAGAGTTTTGCCAATGCAATGTACAATGCCTGCCGGGATGTGGAGGCCCCCTCAAGTAATGACAAGGCCCTGGGACTC
CTGTGTGGGAAGGACGCTGACGCCTGTAATGCCACCAACTGGATTGAATACATGTTCAATAAGGACAATGGACAGGCACCTTTTACCATC
ACTCCTGTGTTTTCAGATTTTCCAGTCCATGGGATGGAGCCCATGAACAATGCCACCAAAGGCTGTGACGAGTCTGTGGATGAGGTCACA
GCACCATGTAGCTGCCAAGACTGCTCTATTGTCTGTGGCCCCAAGCCCCAGCCCCCACCTCCTCCTGCTCCCTGGACGATCCTTGGCTTG
GACGCCATGTATGTCATCATGTGGATCACCTACATGGCGTTTTTGCTTGTGTTTTTTGGAGCATTTTTTGCAGTGTGGTGCTACAGAAAA
CGGTATTTTGTCTCCGAGTACACTCCCATCGATAGCAATATAGCTTTTTCTGTTAATGCAAGTGACAAAGGAGAGGCGTCCTGCTGTGAC
CCTGTCAGCGCAGCATTTGAGGGCTGCTTGAGGCGGCTGTTCACACGCTGGGGGTCTTTCTGCGTCCGAAACCCTGGCTGTGTCATTTTC
TTCTCGCTGGTCTTCATTACTGCGTGTTCGTCAGGCCTGGTGTTTGTCCGGGTCACAACCAATCCAGTTGACCTCTGGTCAGCCCCCAGC
AGCCAGGCTCGCCTGGAAAAAGAGTACTTTGACCAGCACTTTGGGCCTTTCTTCCGGACGGAGCAGCTCATCATCCGGGCCCCTCTCACT
GACAAACACATTTACCAGCCATACCCTTCGGGAGCTGATGTACCCTTTGGACCTCCGCTTGACATACAGATACTGCACCAGGTTCTTGAC
TTACAAATAGCCATCGAAAACATTACTGCCTCTTATGACAATGAGACTGTGACACTTCAAGACATCTGCTTGGCCCCTCTTTCACCGTAT
AACACGAACTGCACCATTTTGAGTGTGTTAAATTACTTCCAGAACAGCCATTCCGTGCTGGACCACAAGAAAGGGGACGACTTCTTTGTG
TATGCCGATTACCACACGCACTTTCTGTACTGCGTACGGGCTCCTGCCTCTCTGAATGATACAAGTTTGCTCCATGACCCTTGTCTGGGT
ACGTTTGGTGGACCAGTGTTCCCGTGGCTTGTGTTGGGAGGCTATGATGATCAAAACTACAATAACGCCACTGCCCTTGTGATTACCTTC
CCTGTCAATAATTACTATAATGATACAGAGAAGCTCCAGAGGGCCCAGGCCTGGGAAAAAGAGTTTATTAATTTTGTGAAAAACTACAAG
AATCCCAATCTGACCATTTCCTTCACTGCTGAACGAAGTATTGAAGATGAACTAAATCGTGAAAGTGACAGTGATGTCTTCACCGTTGTA
ATTAGCTATGCCATCATGTTTCTATATATTTCCCTAGCCTTGGGGCACATGAAAAGCTGTCGCAGGCTTCTGGAGACTAATGAGCAAAAA
CTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAAGAGCTTATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTG
TTGGAAGAAGCAAACCGAGGCTCGTTTCCAGCAGAGATGGTGAATAAAATCTTTTCTAATATTTCATCAATAAATGCCTTCCATAGTAAA
TTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAATGGGAAACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTT
AAGATGTATGGAGAATATGTGAAAGGATTTGATAATGCAATGGAATTGGTTAAAAACATGACAGAACGTATTCCCCAGTTCAAATCAGTG
GTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCTTAACTTTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAG
ATGCTCCTTAAGGACTATCTAAGGAAATTGCCTCCTGATTCCCTGGACTGGAATGATGCTAAAAAATCACTTGAAATTATATCTACAGCA
GCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGAACCTAAAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATT
GTAAACCCTTCAAATGAACTAATAAAAGAAGGACAGATCCTCAAACTAGCTGCTCGGAACACTTCAGCACAAGAACGCTACCTTTTCTTA
TTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCAGCTTGGTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATG
AAAATTGTAGAGACTCAAAATGAAGAATATCCACATACTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAACTGCAGGCCAGTTCTGCG
CAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAACCATCGATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAG
GATAATGACATTCACTCAGAGGTTTCTACTGCTGAGCTAGGGAAAAGAGCCCCAAGATGGATCCGAGATAATGAAGTGACAATGTGTATG
AAATGTAAAGAACCTTTCAATGCACTGACACGAAGGAGGCATCATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTAC
AAAGCTCAACTTGAATATGATGGTGGTAAATTGAGCAAAGTTTGTAAAGACTGTTATCAAATCATAAGTGGATTCACAGACAGTGAAGAA
AAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAGAAGTATCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCA
AAACCTTGGCAGAAAGCTTGGTGTGTGATCCCCAAGCAAGACCCTCTTGTGCTGTACATGTATGGTGCCCCCCAGGACGTCAGAGCCCAG
GCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAATGCCAAGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCC
GTGCACAGCTTTGCTGCAGACAGTGAGGAACTGAAGCAGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACAGGTGAGACACCAGGTGGT
CCAAATGAGCATCCAGCCACCTTGGATGATCATCCTGAACCTAAGAAAAAATCAGAATGCTGAACTCCTCCAGGACCAGCCATGGTGTGG
AGGTCTCAGGACTTACAGCTCAAGACATTCCCAGCTCTTCTTACACATCTGCTAGCACTTTATGTTGAAAAATATAGGCCCATAAATGCA
TCTTTTGAGGACTATTTTCCTATGTTTATGTACTCTTAGTGAAATTAGTGTGCAGAGTCATTCTACCGATAAAGTTTTGAAATAATGTGA
AAACTGGAGCATTTTTTGAGCTATTCCTTGAATATGTGCTTTTTTGTCTTGAAGAAATGGTGTATCAATTGATTCTGTCACCGTCAGGTT
AGAATGAGCACTTCCATTTAAGAAATCCTTTCATGTCTTCTTCTCTTTCACATGTAGGACCTGGAACAGTTTGAAAGATATACCTCCATG
TTGCCAAAATAGATCCATGGTGAAAAATACAGGGACAGTTGAGGCTATTGTATTAACTTATTTAATTTAGTTTATAAATCTCTAGCTGCA
TAAATGATGTCTGTTCTTTTAAAACAAAAGAAAAAGGACAAATTGTTGGTGTTCAGATTTCCGATTTATAAAGAAAAGATAACTTGTTTT
TGTAGAAATACTCCTAAGAATGTCTTAAGTATATAGCAATTATGTATATATAGTATTAAATATATATATTATACATGCTTTTAGGTTCAC
ATTCATCTCTAATTTATTTTTTAAATATAAAGCATGGTTTATCGTGGAATTGAGCAATGTTCTAAACTGAAGAAATGTTTTGGTGATTTA
TGTTTTGCTGATTGGCATTTGAGGGTATTGATATTTTTATAATAAGCGGTAATTTATGTGGCATGGGATATATTTGTGAATTCCAACAGT
ACTTTTAAAGTACCATTTTTTGTTTCTGTGCCTATTTAAAACAGTGCCTCTCTTAGAAGGTGCTATTAATATAAGATGAGGGTTCAGTTA
ATGCTTCAGTCTTGATTATTCTAAGATTAAAGTGCATTTTGGTCACTGGGACAGTCAAAGTCATACAGGTTATTTTACCGTTTACAATCC
TATAATTATTGCAGTAGTTAACATCAGCATGTACAAACATTTTCACCAAAACCCAAACTTTATGAGACACCTGACCTTTTACAGAACTAG
GATATGTGGAAACCACATTTATCGTAATGTTTTTCTTTTCCGTAAGATAATCGAGCATGGTTAAATAAGGTCTCTATATAATGAACACTT
AAGATAACTGTATGGGCCTTTTCCTTTGTCGGAATGAAACAAAATCCACATGTGATAACCTATGTTTGGGGTGGGGGCTGGTCACATGAT
TCCTTGTCATGAAGGCTGCTCTTCACTCTTTTGTACTTGTGATTAAAATCTCACACTAAGTTTACTATTTAACTTAATCCTTTTTCTCAA
ATTAAACCTTTTTTTATACTGTCATACCCCAAACATTGTCACTTTTTAATGTACCAGTCTGCTAGAAATCTACAAGAATGATCCCACTAT
CTTTAGTATGTAAGGATTACACCTTAGATATAGAATAGAATTTGAGACTGCCACACATTTATTCAGGCCTTGTTACTTTAAGAAATATCT
TATTTTTTCCACCCCAAAGGAGCAAGTGAACCAATTATTGCTGACATAATAGGTTCCCTTCCTCCATAAAAGGATTATCAGCCATGATCA
TTAACATTAAACCAAATAGATGGAGCCAAAAAGAGATAAAAGTAGCTTCTTAGGCCCTATTAAATCAGGGAAAGACTAGAAGAAAATTTA
CATTGCTTCTCTTTTTTAATGGCCAGCGGGGTATGTGTGTGATGTATACTCAGAGTGGTTTTTTGGAAAATTAAACTGTAGCTATAGATG
ATGCTTTGGTTTTCTTAATCTCAAGAAAAGAGAAGCAATGGAAATACAGAATGATTAACTGGGGAAGAGGACTTTTGTAAGTGTGACTAG
AACAATGGTAATGTATGGGCCAATGGGAAGATTTATAAGTCAGATTATTTTTGAAAACTATTTTTAAAAGCAAGAAGACTACACTTTCCC
TACCAAAACAGAAGTAACATGGACACACTTAAGTTTTGGCTCAATAAATATCAATGTTATTTAACTTAGATCATTGAAAGAATACTCATG
TAAAGATAATGTTTATTATCATGCTCTTAACAGTCATTATCTTTAATTTTTTAAATGAGAAGCATTAATACTAGAAGAGAATTTCTGGTT
ATCCGGTCACCATATTCACTTTCCACCCCACATTCTTCAGCTAAACGCAAAGAGAAGCAGTGAAACAGCCTTACCCGCTTCTCTCTTATT
AAAGAATCACTGATGTTTTTACTCAATGAAAGGTAAAGTAATACCCTTAGCCATTTATTAAACAATTCAACCAAGAGACCTCAAAGTGTG
ATTGATGATAAGAATAATCAATGCCTTTTCCCTTCCAACATACTTGAGCAGTCATGACAACCTAAAAATATCATTGGTTGCTTTCCCATT
AAAACCAACGTTCCCTGTGGGTCTTAATTCTTTATTTTCACTCATTTGGTGCTTTCCAAGTCATCTTTTCTTTAAAGTGCCCTTCCTCCA
AACTTTAAAAAGTACTTCCTTGACAAAATTTCTATTCATTATAAAACATTTCAATATTTTAAGCTTAAATTTTGCTTTTGCTGAAAGCCT
ACCATTTGGCGTGTTAAGTATGAAATATAGTGCAGACTTTTATCTTGGTTTTAAGTGGGGCTCAATAAAAAACACCAGCCACTTTTGTAA
TAATGGCATCACAGTGTCATCATGTATGCAAGCAATAAAACTCTTTAGGGTATGGTTTTATACTGAAAATTTAATATGAAGGCCCCTTCC
CACAAGAATATAGATAATTATTAACTTTCTAGATGTGATACGGTAATTCGAATTGCAGAGTATAAGGAAGGGAAATGGGAAGGGGCATCA
TTCCTTGGATTTTAAATAACTATCAAGAACATTTTTACTTTAACAAAGAAAATGGATAGAAAAAGCACATTTTGTTTTCCCTGAAGTTTA
ATTTGACATCAGGTTTGTGTACTTATCTTCACTAGGTGACTTAACTTACCCCAATTTTTTTAAAAATTATTAAACTTTTTACAGAAACTA
ACCTTTTAAATGTACCCTTTCCCCATATATATATACGCACACGTTTGGATTTTTTTTTTTTAAGAACACTTGTTCTAGTTATAAATATAT
AAAGAAAACGATAAAGTTTGTGGTACTGCAGGGTTGTTAAAGATTCTTTGATGCCTTCTAAAAACTTTTGTCAAAAATACTTTTGAGTTC
ACAATTCTGTTTTACTTTTCCTTGTCCTTACTTTTTGGAAACAGGGTGGTTGCTTTTATTTGTTTTCTGGTTATATTCAAAGCCTTAAGT
TCTTAATCTGAGCATATTGTCTGTGATAAATTTCTGATGATCTTTCTGGACTAGATACAACCTGAGTAGCAAGCACCAACCGGAGCAAGT
AAACTTCTAGGGAACAAGCGTCTTGGGTTTTATAGGTATCTTTGCTATAATGCAGAATAGATTAATGAAGATTTCCTATATCATATGATA
TTTGTGTTAGTGGGTCTAAGATTAAGCACATGATATTTATAAGCTAAAATTAACTCAAAAGTCAAGAATGTCTTAATGTTTTCATTCTTA
AATTTTGTATTCTCCAAGAATGTATTAGTATATGAACTGTGGCCAACCAGTTCCTATTCTTCAGACTGTATTGACATCTGTAGTGGATCA
TGTTGCTTCTTCATTCTTACCAATTTTATTAGAATCAAACTTCTTGTTATTTGCATACTATTATCTACTATAGATTCTCAGCTTTAGAAA
ATGACTATGATACATAAAGACCACTAGGTCAACTTAAAAAACCCTTTCTGTGAATTTATACATGTATGTATATATGTAAAAACACTGTTG
ATTTGCAATTCTGTCTTTCCATAGAAATGAACTTTTTCTATCGAAATTGTTTAACTTAAATATTTTAACATAAATTATTTACATGGATCT
TTATGTATAATTCATCCTTATATATACCCCTTAATCACGTAGTCATGAGAAGATAACTTTGCTTTCTTTACAGAAAGGGCAGAGAGGAAT
AAGATAAGAAACTGAAACAAGCAAGAATGAAGAGAGATGTGGGGGAGAGACTGCTGGTCTCCAGACCACAGCAATGTGTTTTAAGATAAG
ATGAAATATTTTAACTGCAGAAGGGATATAAAATCTATGTAATTACATGCTGATGGGATCCATTGCACCCAGGTTTTTGACCTTGGCCTG
TAAATGCTAGACTATGATATCTTGTTATTCTTTTTCTCCTTTGGGCTTTTAAAAAATAATTTCATTCTCAGATCATTTTCTGTACTGTTT
ACTGAGGCAAAAAAAAAAAAAATCTGAAGTCAATCATGGTCTTCTACTTTCTGGACTGAGCATTTGGCAGAATGCAGTATCTTTTCCTGT
ATTTTGACATGAAATAGCACATGGCTTCTACAAGATAGTTTTAACTTGTTGGGGTCACCGGGAGTTATATGATGGTCAACCCCTTTTCCC
AAAATTCATTGTGGTAGTTTTAGTGGAAAACGTAAATCAAGAAATCTCATATCATACTTTAATAAATAAATACCAAATACATAGTGACAT
ATAGGTTTGGGAAGAAACTAGTCTGTGGGGACCATTATAAGAGAATCACATTATATATTACACAGTATATGGATATTGGAATGTATCACT
TGTGGGGGGTTCTCTTCATTAGCAAAACAGTCATGTCTGTCTGTATATAAGACTTTTTTTTTTTTAACCAAACTAGCATTTCATTTTGTG
AGTGACAATTGACATTTTAAAATAAGCATAGGCCGGGCATAGTGGCTCATGCCTGTAATCCCAGCCCTTGGGAGGCCGAGGTGGGCAGAT
CACTTCAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAATAAAAATAAAACAAAATAAGTGTATCTG
GGCCAGGCACGGTAGCCCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGAGTTCGAGACCAGCCTGGCCAACATGGCAAAACCCTGT
CTCTACAAAAATTAGCCAGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGTTGAGGCAGGAGAATTGCTTGAACCCGGGAG
ACAGAGGTTGCAGTGAGCCGAGATTGCACCACTGCACTCCGGCCTGGGTGACAGAGCAAGACTTCGACTCAAAAAATAAAATAAGCGTAT
CTGGATTTTTCTTTTGAATTCTTGACTGATAAGTTTGAGAATGTATGTTTTTGGAATTTTACAGTTGACTCAAGTGTGAAAGTATACACA
AGTAATTTTTCTTTTCTAGCTATGTGAATGTAAAATACTTGCAGATAATGTATATATTGTGTAATATATGTATATTTGGTCATAAAAGCA
GATAATTGGAAACATTGTAAAATTAAGCACTTTAATTCCTATTAAATATTAAACATTTGTATCTTGTAAATAAACACATCCTTAAATTAA

>59853_59853_1_NPC1-FGD4_NPC1_chr18_21124924_ENST00000269228_FGD4_chr12_32751431_ENST00000427716_length(amino acids)=1394AA_BP=828
MGWEEKSLSVIVGAEINRDSAAGRDLTRKSPRTALPFAETSPAPSNPETKRESITARNRWHNSTDTLPGRARLHKSRAWPPGIAGAEEKG
NTGTLKRGRGGAPARARESRQRHLPERAEPTTPSSFLTGARSLLPRSAPAPAPPLLLRGVLKQPGEVEPPPGSPTSRTPPASAALRGHSM
TARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPL
QFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDA
DACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVI
MWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFI
TACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIE
NITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPV
FPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIM
FLYISLALGHMKSCRRLLETNEQKLHKIANELLLTERAYVNRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPEL
EKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNMTERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDY
LRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLL
YCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTFQVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHS
EVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGI
LEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHSFAA

--------------------------------------------------------------
>59853_59853_2_NPC1-FGD4_NPC1_chr18_21124924_ENST00000269228_FGD4_chr12_32751431_ENST00000531134_length(transcript)=4409nt_BP=2502nt
CAGATTCCTTTCAGGTGACTGGGTTGGGAGGAGAAGTCGCTCAGCGTAATTGTCGGAGCCGAAATAAACAGAGACTCCGCGGCCGGGCGG
GACCTTACCCGGAAGTCCCCCAGAACAGCACTACCCTTCGCTGAAACCTCGCCCGCCCCCTCCAACCCAGAGACGAAAAGGGAAAGCATC
ACGGCCAGAAACCGTTGGCACAACTCCACAGATACTCTCCCGGGCCGAGCCAGACTCCATAAGTCCCGCGCCTGGCCCCCGGGGATTGCA
GGGGCTGAGGAGAAGGGCAACACGGGGACCTTGAAGCGGGGTCGCGGCGGCGCCCCAGCCCGGGCCAGGGAGTCCCGGCAGCGGCACCTC
CCAGAAAGGGCGGAGCCGACGACGCCTTCTTCCTTCCTGACCGGCGCGCGCAGCCTGCTGCCGCGGTCAGCGCCTGCTCCTGCTCCTCCG
CTCCTCCTGCGCGGGGTGCTGAAACAGCCCGGGGAAGTAGAGCCGCCTCCGGGGAGCCCAACCAGCCGAACGCCGCCGGCGTCAGCAGCC
TTGCGCGGCCACAGCATGACCGCTCGCGGCCTGGCCCTTGGCCTCCTCCTGCTGCTACTGTGTCCAGCGCAGGTGTTTTCACAGTCCTGT
GTTTGGTATGGAGAGTGTGGAATTGCATATGGGGACAAGAGGTACAATTGCGAATATTCTGGCCCACCAAAACCATTGCCAAAGGATGGA
TATGACTTAGTGCAGGAACTCTGTCCAGGATTCTTCTTTGGCAATGTCAGTCTCTGTTGTGATGTTCGGCAGCTTCAGACACTAAAAGAC
AACCTGCAGCTGCCTCTACAGTTTCTGTCCAGATGTCCATCCTGTTTTTATAACCTACTGAACCTGTTTTGTGAGCTGACATGTAGCCCT
CGACAGAGTCAGTTTTTGAATGTTACAGCTACTGAAGATTATGTTGATCCTGTTACAAACCAGACGAAAACAAATGTGAAAGAGTTACAA
TACTACGTCGGACAGAGTTTTGCCAATGCAATGTACAATGCCTGCCGGGATGTGGAGGCCCCCTCAAGTAATGACAAGGCCCTGGGACTC
CTGTGTGGGAAGGACGCTGACGCCTGTAATGCCACCAACTGGATTGAATACATGTTCAATAAGGACAATGGACAGGCACCTTTTACCATC
ACTCCTGTGTTTTCAGATTTTCCAGTCCATGGGATGGAGCCCATGAACAATGCCACCAAAGGCTGTGACGAGTCTGTGGATGAGGTCACA
GCACCATGTAGCTGCCAAGACTGCTCTATTGTCTGTGGCCCCAAGCCCCAGCCCCCACCTCCTCCTGCTCCCTGGACGATCCTTGGCTTG
GACGCCATGTATGTCATCATGTGGATCACCTACATGGCGTTTTTGCTTGTGTTTTTTGGAGCATTTTTTGCAGTGTGGTGCTACAGAAAA
CGGTATTTTGTCTCCGAGTACACTCCCATCGATAGCAATATAGCTTTTTCTGTTAATGCAAGTGACAAAGGAGAGGCGTCCTGCTGTGAC
CCTGTCAGCGCAGCATTTGAGGGCTGCTTGAGGCGGCTGTTCACACGCTGGGGGTCTTTCTGCGTCCGAAACCCTGGCTGTGTCATTTTC
TTCTCGCTGGTCTTCATTACTGCGTGTTCGTCAGGCCTGGTGTTTGTCCGGGTCACAACCAATCCAGTTGACCTCTGGTCAGCCCCCAGC
AGCCAGGCTCGCCTGGAAAAAGAGTACTTTGACCAGCACTTTGGGCCTTTCTTCCGGACGGAGCAGCTCATCATCCGGGCCCCTCTCACT
GACAAACACATTTACCAGCCATACCCTTCGGGAGCTGATGTACCCTTTGGACCTCCGCTTGACATACAGATACTGCACCAGGTTCTTGAC
TTACAAATAGCCATCGAAAACATTACTGCCTCTTATGACAATGAGACTGTGACACTTCAAGACATCTGCTTGGCCCCTCTTTCACCGTAT
AACACGAACTGCACCATTTTGAGTGTGTTAAATTACTTCCAGAACAGCCATTCCGTGCTGGACCACAAGAAAGGGGACGACTTCTTTGTG
TATGCCGATTACCACACGCACTTTCTGTACTGCGTACGGGCTCCTGCCTCTCTGAATGATACAAGTTTGCTCCATGACCCTTGTCTGGGT
ACGTTTGGTGGACCAGTGTTCCCGTGGCTTGTGTTGGGAGGCTATGATGATCAAAACTACAATAACGCCACTGCCCTTGTGATTACCTTC
CCTGTCAATAATTACTATAATGATACAGAGAAGCTCCAGAGGGCCCAGGCCTGGGAAAAAGAGTTTATTAATTTTGTGAAAAACTACAAG
AATCCCAATCTGACCATTTCCTTCACTGCTGAACGAAGTATTGAAGATGAACTAAATCGTGAAAGTGACAGTGATGTCTTCACCGTTGTA
ATTAGCTATGCCATCATGTTTCTATATATTTCCCTAGCCTTGGGGCACATGAAAAGCTGTCGCAGGCTTCTGGAGACTAATGAGCAAAAA
CTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAAGAGCTTATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTG
TTGGAAGAAGCAAACCGAGGCTCGTTTCCAGCAGAGATGGTGAATAAAATCTTTTCTAATATTTCATCAATAAATGCCTTCCATAGTAAA
TTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAATGGGAAACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTT
AAGATGTATGGAGAATATGTGAAAGGATTTGATAATGCAATGGAATTGGTTAAAAACATGACAGAACGTATTCCCCAGTTCAAATCAGTG
GTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCTTAACTTTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAG
ATGCTCCTTAAGGACTATCTAAGGAAATTGCCTCCTGATTCCCTGGACTGGAATGATGCTAAAAAATCACTTGAAATTATATCTACAGCA
GCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGAACCTAAAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATT
GTAAACCCTTCAAATGAACTAATAAAAGAAGGACAGATCCTCAAACTAGCTGCTCGGAACACTTCAGCACAAGAACGCTACCTTTTCTTA
TTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCAGCTTGGTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATG
AAAATTGTAGAGACTCAAAATGAAGAATATCCACATACTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAACTGCAGGCCAGTTCTGCG
CAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAACCATCGATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAG
GATAATGACATTCACTCAGAGGTTTCTACTGCTGAGCTAGGGAAAAGAGCCCCAAGATGGATCCGAGATAATGAAGTGACAATGTGTATG
AAATGTAAAGAACCTTTCAATGCACTGACACGAAGGAGGCATCATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTAC
AAAGCTCAACTTGAATATGATGGTGGTAAATTGAGCAAAGTTTGTAAAGACTGTTATCAAATCATAAGTGGATTCACAGACAGTGAAGAA
AAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAGAAGTATCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCA
AAACCTTGGCAGAAAGCTTGGTGTGTGATCCCCAAGCAAGACCCTCTTGTGCTGTACATGTATGGTGCCCCCCAGGACGTCAGAGCCCAG
GCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAATGCCAAGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCC
GTGCACAGCTTTGCTGCAGACAGTGAGGAACTGAAGCAGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACAGGTGAGACACCAGGTGGT
CCAAATGAGCATCCAGCCACCTTGGATGATCATCCTGAACCTAAGAAAAAATCAGAATGCTGAACTCCTCCAGGACCAGCCATGGTGTGG
AGGTCTCAGGACTTACAGCTCAAGACATTCCCAGCTCTTCTTACACATCTGCTAGCACTTTATGTTGAAAAATATAGGCCCATAAATGCA

>59853_59853_2_NPC1-FGD4_NPC1_chr18_21124924_ENST00000269228_FGD4_chr12_32751431_ENST00000531134_length(amino acids)=1394AA_BP=828
MGWEEKSLSVIVGAEINRDSAAGRDLTRKSPRTALPFAETSPAPSNPETKRESITARNRWHNSTDTLPGRARLHKSRAWPPGIAGAEEKG
NTGTLKRGRGGAPARARESRQRHLPERAEPTTPSSFLTGARSLLPRSAPAPAPPLLLRGVLKQPGEVEPPPGSPTSRTPPASAALRGHSM
TARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPL
QFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDA
DACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVI
MWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFI
TACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIE
NITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPV
FPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIM
FLYISLALGHMKSCRRLLETNEQKLHKIANELLLTERAYVNRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPEL
EKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNMTERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDY
LRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLL
YCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTFQVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHS
EVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGI
LEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHSFAA

--------------------------------------------------------------
>59853_59853_3_NPC1-FGD4_NPC1_chr18_21124924_ENST00000269228_FGD4_chr12_32751431_ENST00000534526_length(transcript)=4203nt_BP=2502nt
CAGATTCCTTTCAGGTGACTGGGTTGGGAGGAGAAGTCGCTCAGCGTAATTGTCGGAGCCGAAATAAACAGAGACTCCGCGGCCGGGCGG
GACCTTACCCGGAAGTCCCCCAGAACAGCACTACCCTTCGCTGAAACCTCGCCCGCCCCCTCCAACCCAGAGACGAAAAGGGAAAGCATC
ACGGCCAGAAACCGTTGGCACAACTCCACAGATACTCTCCCGGGCCGAGCCAGACTCCATAAGTCCCGCGCCTGGCCCCCGGGGATTGCA
GGGGCTGAGGAGAAGGGCAACACGGGGACCTTGAAGCGGGGTCGCGGCGGCGCCCCAGCCCGGGCCAGGGAGTCCCGGCAGCGGCACCTC
CCAGAAAGGGCGGAGCCGACGACGCCTTCTTCCTTCCTGACCGGCGCGCGCAGCCTGCTGCCGCGGTCAGCGCCTGCTCCTGCTCCTCCG
CTCCTCCTGCGCGGGGTGCTGAAACAGCCCGGGGAAGTAGAGCCGCCTCCGGGGAGCCCAACCAGCCGAACGCCGCCGGCGTCAGCAGCC
TTGCGCGGCCACAGCATGACCGCTCGCGGCCTGGCCCTTGGCCTCCTCCTGCTGCTACTGTGTCCAGCGCAGGTGTTTTCACAGTCCTGT
GTTTGGTATGGAGAGTGTGGAATTGCATATGGGGACAAGAGGTACAATTGCGAATATTCTGGCCCACCAAAACCATTGCCAAAGGATGGA
TATGACTTAGTGCAGGAACTCTGTCCAGGATTCTTCTTTGGCAATGTCAGTCTCTGTTGTGATGTTCGGCAGCTTCAGACACTAAAAGAC
AACCTGCAGCTGCCTCTACAGTTTCTGTCCAGATGTCCATCCTGTTTTTATAACCTACTGAACCTGTTTTGTGAGCTGACATGTAGCCCT
CGACAGAGTCAGTTTTTGAATGTTACAGCTACTGAAGATTATGTTGATCCTGTTACAAACCAGACGAAAACAAATGTGAAAGAGTTACAA
TACTACGTCGGACAGAGTTTTGCCAATGCAATGTACAATGCCTGCCGGGATGTGGAGGCCCCCTCAAGTAATGACAAGGCCCTGGGACTC
CTGTGTGGGAAGGACGCTGACGCCTGTAATGCCACCAACTGGATTGAATACATGTTCAATAAGGACAATGGACAGGCACCTTTTACCATC
ACTCCTGTGTTTTCAGATTTTCCAGTCCATGGGATGGAGCCCATGAACAATGCCACCAAAGGCTGTGACGAGTCTGTGGATGAGGTCACA
GCACCATGTAGCTGCCAAGACTGCTCTATTGTCTGTGGCCCCAAGCCCCAGCCCCCACCTCCTCCTGCTCCCTGGACGATCCTTGGCTTG
GACGCCATGTATGTCATCATGTGGATCACCTACATGGCGTTTTTGCTTGTGTTTTTTGGAGCATTTTTTGCAGTGTGGTGCTACAGAAAA
CGGTATTTTGTCTCCGAGTACACTCCCATCGATAGCAATATAGCTTTTTCTGTTAATGCAAGTGACAAAGGAGAGGCGTCCTGCTGTGAC
CCTGTCAGCGCAGCATTTGAGGGCTGCTTGAGGCGGCTGTTCACACGCTGGGGGTCTTTCTGCGTCCGAAACCCTGGCTGTGTCATTTTC
TTCTCGCTGGTCTTCATTACTGCGTGTTCGTCAGGCCTGGTGTTTGTCCGGGTCACAACCAATCCAGTTGACCTCTGGTCAGCCCCCAGC
AGCCAGGCTCGCCTGGAAAAAGAGTACTTTGACCAGCACTTTGGGCCTTTCTTCCGGACGGAGCAGCTCATCATCCGGGCCCCTCTCACT
GACAAACACATTTACCAGCCATACCCTTCGGGAGCTGATGTACCCTTTGGACCTCCGCTTGACATACAGATACTGCACCAGGTTCTTGAC
TTACAAATAGCCATCGAAAACATTACTGCCTCTTATGACAATGAGACTGTGACACTTCAAGACATCTGCTTGGCCCCTCTTTCACCGTAT
AACACGAACTGCACCATTTTGAGTGTGTTAAATTACTTCCAGAACAGCCATTCCGTGCTGGACCACAAGAAAGGGGACGACTTCTTTGTG
TATGCCGATTACCACACGCACTTTCTGTACTGCGTACGGGCTCCTGCCTCTCTGAATGATACAAGTTTGCTCCATGACCCTTGTCTGGGT
ACGTTTGGTGGACCAGTGTTCCCGTGGCTTGTGTTGGGAGGCTATGATGATCAAAACTACAATAACGCCACTGCCCTTGTGATTACCTTC
CCTGTCAATAATTACTATAATGATACAGAGAAGCTCCAGAGGGCCCAGGCCTGGGAAAAAGAGTTTATTAATTTTGTGAAAAACTACAAG
AATCCCAATCTGACCATTTCCTTCACTGCTGAACGAAGTATTGAAGATGAACTAAATCGTGAAAGTGACAGTGATGTCTTCACCGTTGTA
ATTAGCTATGCCATCATGTTTCTATATATTTCCCTAGCCTTGGGGCACATGAAAAGCTGTCGCAGGCTTCTGGAGACTAATGAGCAAAAA
CTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAAGAGCTTATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTG
TTGGAAGAAGCAAACCGAGGCTCGTTTCCAGCAGAGATGGTGAATAAAATCTTTTCTAATATTTCATCAATAAATGCCTTCCATAGTAAA
TTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAATGGGAAACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTT
AAGATGTATGGAGAATATGTGAAAGGATTTGATAATGCAATGGAATTGGTTAAAAACATGACAGAACGTATTCCCCAGTTCAAATCAGTG
GTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCTTAACTTTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAG
ATGCTCCTTAAGGACTATCTAAGGAAATTGCCTCCTGATTCCCTGGACTGGAATGATGCTAAAAAATCACTTGAAATTATATCTACAGCA
GCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGAACCTAAAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATT
GTAAACCCTTCAAATGAACTAATAAAAGAAGGACAGATCCTCAAACTAGCTGCTCGGAACACTTCAGCACAAGAACGCTACCTTTTCTTA
TTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCAGCTTGGTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATG
AAAATTGTAGAGACTCAAAATGAAGAATATCCACATACTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAACTGCAGGCCAGTTCTGCG
CAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAACCATCGATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAG
GATAATGACATTCACTCAGAGGTTTCTACTGCTGAGCTAGGGAAAAGAGCCCCAAGATGGATCCGAGATAATGAAGTGACAATGTGTATG
AAATGTAAAGAACCTTTCAATGCACTGACACGAAGGAGGCATCATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTAC
AAAGCTCAACTTGAATATGATGGTGGTAAATTGAGCAAAGTTTGTAAAGACTGTTATCAAATCATAAGTGGATTCACAGACAGTGAAGAA
AAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAGAAGTATCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCA
AAACCTTGGCAGAAAGCTTGGTGTGTGATCCCCAAGCAAGACCCTCTTGTGCTGTACATGTATGGTGCCCCCCAGGACGTCAGAGCCCAG
GCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAATGCCAAGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCC
GTGCACAGCTTTGCTGCAGACAGTGAGGAACTGAAGCAGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACAGGTGAGACACCAGGTGGT

>59853_59853_3_NPC1-FGD4_NPC1_chr18_21124924_ENST00000269228_FGD4_chr12_32751431_ENST00000534526_length(amino acids)=1394AA_BP=828
MGWEEKSLSVIVGAEINRDSAAGRDLTRKSPRTALPFAETSPAPSNPETKRESITARNRWHNSTDTLPGRARLHKSRAWPPGIAGAEEKG
NTGTLKRGRGGAPARARESRQRHLPERAEPTTPSSFLTGARSLLPRSAPAPAPPLLLRGVLKQPGEVEPPPGSPTSRTPPASAALRGHSM
TARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPL
QFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDA
DACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVI
MWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFI
TACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIE
NITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPV
FPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIM
FLYISLALGHMKSCRRLLETNEQKLHKIANELLLTERAYVNRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPEL
EKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNMTERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDY
LRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLL
YCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTFQVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHS
EVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGI
LEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHSFAA

--------------------------------------------------------------
>59853_59853_4_NPC1-FGD4_NPC1_chr18_21124924_ENST00000412552_FGD4_chr12_32751431_ENST00000427716_length(transcript)=8243nt_BP=1025nt
CTGCTCCCTGGACGATCCTTGGCTTGGACGCCATGTATGTCATCATGTGGATCACCTACATGGCGTTTTTGCTTGTGTTTTTTGGAGCAT
TTTTTGCAGTGTGGTGCTACAGAAAACGGTATTTTGTCTCCGAGTACACTCCCATCGATAGCAATATAGCTTTTTCTGTTAATGCAAGTG
ACAAAGGAACGGCTTGGCTCTTAACCTCCACCTTCCCTTCCTCTCCCGTTCTTCCAGGAGAGGCGTCCTGCTGTGACCCTGTCAGCGCAG
CATTTGAGGGCTGCTTGAGGCGGCTGTTCACACGCTGGGGGTCTTTCTGCGTCCGAAACCCTGGCTGTGTCATTTTCTTCTCGCTGGTCT
TCATTACTGCGTGTTCGTCAGGCCTGGTGTTTGTCCGGGTCACAACCAATCCAGTTGACCTCTGGTCAGCCCCCAGCAGCCAGGCTCGCC
TGGAAAAAGAGTACTTTGACCAGCACTTTGGGCCTTTCTTCCGGACGGAGCAGCTCATCATCCGGGCCCCTCTCACTGACAAACACATTT
ACCAGCCATACCCTTCGGGAGCTGATGTACCCTTTGGACCTCCGCTTGACATACAGATACTGCACCAGGTTCTTGACTTACAAATAGCCA
TCGAAAACATTACTGCCTCTTATGACAATGAGACTGTGACACTTCAAGACATCTGCTTGGCCCCTCTTTCACCGTATAACACGAACTGCA
CCATTTTGAGTGTGTTAAATTACTTCCAGAACAGCCATTCCGTGCTGGACCACAAGAAAGGGGACGACTTCTTTGTGTATGCCGATTACC
ACACGCACTTTCTGTACTGCGTACGGTTTATTAATTTTGTGAAAAACTACAAGAATCCCAATCTGACCATTTCCTTCACTGCTGAACGAA
GTATTGAAGATGAACTAAATCGTGAAAGTGACAGTGATGTCTTCACCGTTGTAATTAGCTATGCCATCATGTTTCTATATATTTCCCTAG
CCTTGGGGCACATGAAAAGCTGTCGCAGGCTTCTGGAGACTAATGAGCAAAAACTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAA
GAGCTTATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTGTTGGAAGAAGCAAACCGAGGCTCGTTTCCAGCAGAGA
TGGTGAATAAAATCTTTTCTAATATTTCATCAATAAATGCCTTCCATAGTAAATTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAAT
GGGAAACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTTAAGATGTATGGAGAATATGTGAAAGGATTTGATAATG
CAATGGAATTGGTTAAAAACATGACAGAACGTATTCCCCAGTTCAAATCAGTGGTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCT
TAACTTTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAGATGCTCCTTAAGGACTATCTAAGGAAATTGCCTCCTG
ATTCCCTGGACTGGAATGATGCTAAAAAATCACTTGAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGA
ACCTAAAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATTGTAAACCCTTCAAATGAACTAATAAAAGAAGGACAGA
TCCTCAAACTAGCTGCTCGGAACACTTCAGCACAAGAACGCTACCTTTTCTTATTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCA
GCTTGGTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATGAAAATTGTAGAGACTCAAAATGAAGAATATCCACATA
CTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAACTGCAGGCCAGTTCTGCGCAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAA
CCATCGATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAGGATAATGACATTCACTCAGAGGTTTCTACTGCTGAGC
TAGGGAAAAGAGCCCCAAGATGGATCCGAGATAATGAAGTGACAATGTGTATGAAATGTAAAGAACCTTTCAATGCACTGACACGAAGGA
GGCATCATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTACAAAGCTCAACTTGAATATGATGGTGGTAAATTGAGCA
AAGTTTGTAAAGACTGTTATCAAATCATAAGTGGATTCACAGACAGTGAAGAAAAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAG
AAGTATCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCAAAACCTTGGCAGAAAGCTTGGTGTGTGATCCCCAAGC
AAGACCCTCTTGTGCTGTACATGTATGGTGCCCCCCAGGACGTCAGAGCCCAGGCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAA
TGCCAAGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCCGTGCACAGCTTTGCTGCAGACAGTGAGGAACTGAAGC
AGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACAGGTGAGACACCAGGTGGTCCAAATGAGCATCCAGCCACCTTGGATGATCATCCTG
AACCTAAGAAAAAATCAGAATGCTGAACTCCTCCAGGACCAGCCATGGTGTGGAGGTCTCAGGACTTACAGCTCAAGACATTCCCAGCTC
TTCTTACACATCTGCTAGCACTTTATGTTGAAAAATATAGGCCCATAAATGCATCTTTTGAGGACTATTTTCCTATGTTTATGTACTCTT
AGTGAAATTAGTGTGCAGAGTCATTCTACCGATAAAGTTTTGAAATAATGTGAAAACTGGAGCATTTTTTGAGCTATTCCTTGAATATGT
GCTTTTTTGTCTTGAAGAAATGGTGTATCAATTGATTCTGTCACCGTCAGGTTAGAATGAGCACTTCCATTTAAGAAATCCTTTCATGTC
TTCTTCTCTTTCACATGTAGGACCTGGAACAGTTTGAAAGATATACCTCCATGTTGCCAAAATAGATCCATGGTGAAAAATACAGGGACA
GTTGAGGCTATTGTATTAACTTATTTAATTTAGTTTATAAATCTCTAGCTGCATAAATGATGTCTGTTCTTTTAAAACAAAAGAAAAAGG
ACAAATTGTTGGTGTTCAGATTTCCGATTTATAAAGAAAAGATAACTTGTTTTTGTAGAAATACTCCTAAGAATGTCTTAAGTATATAGC
AATTATGTATATATAGTATTAAATATATATATTATACATGCTTTTAGGTTCACATTCATCTCTAATTTATTTTTTAAATATAAAGCATGG
TTTATCGTGGAATTGAGCAATGTTCTAAACTGAAGAAATGTTTTGGTGATTTATGTTTTGCTGATTGGCATTTGAGGGTATTGATATTTT
TATAATAAGCGGTAATTTATGTGGCATGGGATATATTTGTGAATTCCAACAGTACTTTTAAAGTACCATTTTTTGTTTCTGTGCCTATTT
AAAACAGTGCCTCTCTTAGAAGGTGCTATTAATATAAGATGAGGGTTCAGTTAATGCTTCAGTCTTGATTATTCTAAGATTAAAGTGCAT
TTTGGTCACTGGGACAGTCAAAGTCATACAGGTTATTTTACCGTTTACAATCCTATAATTATTGCAGTAGTTAACATCAGCATGTACAAA
CATTTTCACCAAAACCCAAACTTTATGAGACACCTGACCTTTTACAGAACTAGGATATGTGGAAACCACATTTATCGTAATGTTTTTCTT
TTCCGTAAGATAATCGAGCATGGTTAAATAAGGTCTCTATATAATGAACACTTAAGATAACTGTATGGGCCTTTTCCTTTGTCGGAATGA
AACAAAATCCACATGTGATAACCTATGTTTGGGGTGGGGGCTGGTCACATGATTCCTTGTCATGAAGGCTGCTCTTCACTCTTTTGTACT
TGTGATTAAAATCTCACACTAAGTTTACTATTTAACTTAATCCTTTTTCTCAAATTAAACCTTTTTTTATACTGTCATACCCCAAACATT
GTCACTTTTTAATGTACCAGTCTGCTAGAAATCTACAAGAATGATCCCACTATCTTTAGTATGTAAGGATTACACCTTAGATATAGAATA
GAATTTGAGACTGCCACACATTTATTCAGGCCTTGTTACTTTAAGAAATATCTTATTTTTTCCACCCCAAAGGAGCAAGTGAACCAATTA
TTGCTGACATAATAGGTTCCCTTCCTCCATAAAAGGATTATCAGCCATGATCATTAACATTAAACCAAATAGATGGAGCCAAAAAGAGAT
AAAAGTAGCTTCTTAGGCCCTATTAAATCAGGGAAAGACTAGAAGAAAATTTACATTGCTTCTCTTTTTTAATGGCCAGCGGGGTATGTG
TGTGATGTATACTCAGAGTGGTTTTTTGGAAAATTAAACTGTAGCTATAGATGATGCTTTGGTTTTCTTAATCTCAAGAAAAGAGAAGCA
ATGGAAATACAGAATGATTAACTGGGGAAGAGGACTTTTGTAAGTGTGACTAGAACAATGGTAATGTATGGGCCAATGGGAAGATTTATA
AGTCAGATTATTTTTGAAAACTATTTTTAAAAGCAAGAAGACTACACTTTCCCTACCAAAACAGAAGTAACATGGACACACTTAAGTTTT
GGCTCAATAAATATCAATGTTATTTAACTTAGATCATTGAAAGAATACTCATGTAAAGATAATGTTTATTATCATGCTCTTAACAGTCAT
TATCTTTAATTTTTTAAATGAGAAGCATTAATACTAGAAGAGAATTTCTGGTTATCCGGTCACCATATTCACTTTCCACCCCACATTCTT
CAGCTAAACGCAAAGAGAAGCAGTGAAACAGCCTTACCCGCTTCTCTCTTATTAAAGAATCACTGATGTTTTTACTCAATGAAAGGTAAA
GTAATACCCTTAGCCATTTATTAAACAATTCAACCAAGAGACCTCAAAGTGTGATTGATGATAAGAATAATCAATGCCTTTTCCCTTCCA
ACATACTTGAGCAGTCATGACAACCTAAAAATATCATTGGTTGCTTTCCCATTAAAACCAACGTTCCCTGTGGGTCTTAATTCTTTATTT
TCACTCATTTGGTGCTTTCCAAGTCATCTTTTCTTTAAAGTGCCCTTCCTCCAAACTTTAAAAAGTACTTCCTTGACAAAATTTCTATTC
ATTATAAAACATTTCAATATTTTAAGCTTAAATTTTGCTTTTGCTGAAAGCCTACCATTTGGCGTGTTAAGTATGAAATATAGTGCAGAC
TTTTATCTTGGTTTTAAGTGGGGCTCAATAAAAAACACCAGCCACTTTTGTAATAATGGCATCACAGTGTCATCATGTATGCAAGCAATA
AAACTCTTTAGGGTATGGTTTTATACTGAAAATTTAATATGAAGGCCCCTTCCCACAAGAATATAGATAATTATTAACTTTCTAGATGTG
ATACGGTAATTCGAATTGCAGAGTATAAGGAAGGGAAATGGGAAGGGGCATCATTCCTTGGATTTTAAATAACTATCAAGAACATTTTTA
CTTTAACAAAGAAAATGGATAGAAAAAGCACATTTTGTTTTCCCTGAAGTTTAATTTGACATCAGGTTTGTGTACTTATCTTCACTAGGT
GACTTAACTTACCCCAATTTTTTTAAAAATTATTAAACTTTTTACAGAAACTAACCTTTTAAATGTACCCTTTCCCCATATATATATACG
CACACGTTTGGATTTTTTTTTTTTAAGAACACTTGTTCTAGTTATAAATATATAAAGAAAACGATAAAGTTTGTGGTACTGCAGGGTTGT
TAAAGATTCTTTGATGCCTTCTAAAAACTTTTGTCAAAAATACTTTTGAGTTCACAATTCTGTTTTACTTTTCCTTGTCCTTACTTTTTG
GAAACAGGGTGGTTGCTTTTATTTGTTTTCTGGTTATATTCAAAGCCTTAAGTTCTTAATCTGAGCATATTGTCTGTGATAAATTTCTGA
TGATCTTTCTGGACTAGATACAACCTGAGTAGCAAGCACCAACCGGAGCAAGTAAACTTCTAGGGAACAAGCGTCTTGGGTTTTATAGGT
ATCTTTGCTATAATGCAGAATAGATTAATGAAGATTTCCTATATCATATGATATTTGTGTTAGTGGGTCTAAGATTAAGCACATGATATT
TATAAGCTAAAATTAACTCAAAAGTCAAGAATGTCTTAATGTTTTCATTCTTAAATTTTGTATTCTCCAAGAATGTATTAGTATATGAAC
TGTGGCCAACCAGTTCCTATTCTTCAGACTGTATTGACATCTGTAGTGGATCATGTTGCTTCTTCATTCTTACCAATTTTATTAGAATCA
AACTTCTTGTTATTTGCATACTATTATCTACTATAGATTCTCAGCTTTAGAAAATGACTATGATACATAAAGACCACTAGGTCAACTTAA
AAAACCCTTTCTGTGAATTTATACATGTATGTATATATGTAAAAACACTGTTGATTTGCAATTCTGTCTTTCCATAGAAATGAACTTTTT
CTATCGAAATTGTTTAACTTAAATATTTTAACATAAATTATTTACATGGATCTTTATGTATAATTCATCCTTATATATACCCCTTAATCA
CGTAGTCATGAGAAGATAACTTTGCTTTCTTTACAGAAAGGGCAGAGAGGAATAAGATAAGAAACTGAAACAAGCAAGAATGAAGAGAGA
TGTGGGGGAGAGACTGCTGGTCTCCAGACCACAGCAATGTGTTTTAAGATAAGATGAAATATTTTAACTGCAGAAGGGATATAAAATCTA
TGTAATTACATGCTGATGGGATCCATTGCACCCAGGTTTTTGACCTTGGCCTGTAAATGCTAGACTATGATATCTTGTTATTCTTTTTCT
CCTTTGGGCTTTTAAAAAATAATTTCATTCTCAGATCATTTTCTGTACTGTTTACTGAGGCAAAAAAAAAAAAAATCTGAAGTCAATCAT
GGTCTTCTACTTTCTGGACTGAGCATTTGGCAGAATGCAGTATCTTTTCCTGTATTTTGACATGAAATAGCACATGGCTTCTACAAGATA
GTTTTAACTTGTTGGGGTCACCGGGAGTTATATGATGGTCAACCCCTTTTCCCAAAATTCATTGTGGTAGTTTTAGTGGAAAACGTAAAT
CAAGAAATCTCATATCATACTTTAATAAATAAATACCAAATACATAGTGACATATAGGTTTGGGAAGAAACTAGTCTGTGGGGACCATTA
TAAGAGAATCACATTATATATTACACAGTATATGGATATTGGAATGTATCACTTGTGGGGGGTTCTCTTCATTAGCAAAACAGTCATGTC
TGTCTGTATATAAGACTTTTTTTTTTTTAACCAAACTAGCATTTCATTTTGTGAGTGACAATTGACATTTTAAAATAAGCATAGGCCGGG
CATAGTGGCTCATGCCTGTAATCCCAGCCCTTGGGAGGCCGAGGTGGGCAGATCACTTCAGGTCAGGAGTTTGAGACCAGCCTGGCCAAC
ATGGTGAAACCCTGTCTCTACTAAATAAAAATAAAACAAAATAAGTGTATCTGGGCCAGGCACGGTAGCCCATGCCTGTAATCCCAGCAC
TTTGGGAGGCCGAGGAGTTCGAGACCAGCCTGGCCAACATGGCAAAACCCTGTCTCTACAAAAATTAGCCAGGTGTGGTGGCACATGCCT
GTAATCCCAGCTACTCGGGAGGTTGAGGCAGGAGAATTGCTTGAACCCGGGAGACAGAGGTTGCAGTGAGCCGAGATTGCACCACTGCAC
TCCGGCCTGGGTGACAGAGCAAGACTTCGACTCAAAAAATAAAATAAGCGTATCTGGATTTTTCTTTTGAATTCTTGACTGATAAGTTTG
AGAATGTATGTTTTTGGAATTTTACAGTTGACTCAAGTGTGAAAGTATACACAAGTAATTTTTCTTTTCTAGCTATGTGAATGTAAAATA
CTTGCAGATAATGTATATATTGTGTAATATATGTATATTTGGTCATAAAAGCAGATAATTGGAAACATTGTAAAATTAAGCACTTTAATT

>59853_59853_4_NPC1-FGD4_NPC1_chr18_21124924_ENST00000412552_FGD4_chr12_32751431_ENST00000427716_length(amino acids)=900AA_BP=334
MDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGTAWLLTSTFPSSPVLPGEASCCDPVSAAFEGCLRR
LFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGA
DVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCV
RFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLETNEQKLHKIANELLLTERAYVNRLD
LLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPELEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNM
TERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDYLRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEI
YEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLLYCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTFQVSGKE
RTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHSEVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACG
YVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGILEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYM
YGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHSFAADSEELKQKWLKVILLAVTGETPGGPNEHPATLDDHPEPKKKSEC

--------------------------------------------------------------
>59853_59853_5_NPC1-FGD4_NPC1_chr18_21124924_ENST00000412552_FGD4_chr12_32751431_ENST00000531134_length(transcript)=2932nt_BP=1025nt
CTGCTCCCTGGACGATCCTTGGCTTGGACGCCATGTATGTCATCATGTGGATCACCTACATGGCGTTTTTGCTTGTGTTTTTTGGAGCAT
TTTTTGCAGTGTGGTGCTACAGAAAACGGTATTTTGTCTCCGAGTACACTCCCATCGATAGCAATATAGCTTTTTCTGTTAATGCAAGTG
ACAAAGGAACGGCTTGGCTCTTAACCTCCACCTTCCCTTCCTCTCCCGTTCTTCCAGGAGAGGCGTCCTGCTGTGACCCTGTCAGCGCAG
CATTTGAGGGCTGCTTGAGGCGGCTGTTCACACGCTGGGGGTCTTTCTGCGTCCGAAACCCTGGCTGTGTCATTTTCTTCTCGCTGGTCT
TCATTACTGCGTGTTCGTCAGGCCTGGTGTTTGTCCGGGTCACAACCAATCCAGTTGACCTCTGGTCAGCCCCCAGCAGCCAGGCTCGCC
TGGAAAAAGAGTACTTTGACCAGCACTTTGGGCCTTTCTTCCGGACGGAGCAGCTCATCATCCGGGCCCCTCTCACTGACAAACACATTT
ACCAGCCATACCCTTCGGGAGCTGATGTACCCTTTGGACCTCCGCTTGACATACAGATACTGCACCAGGTTCTTGACTTACAAATAGCCA
TCGAAAACATTACTGCCTCTTATGACAATGAGACTGTGACACTTCAAGACATCTGCTTGGCCCCTCTTTCACCGTATAACACGAACTGCA
CCATTTTGAGTGTGTTAAATTACTTCCAGAACAGCCATTCCGTGCTGGACCACAAGAAAGGGGACGACTTCTTTGTGTATGCCGATTACC
ACACGCACTTTCTGTACTGCGTACGGTTTATTAATTTTGTGAAAAACTACAAGAATCCCAATCTGACCATTTCCTTCACTGCTGAACGAA
GTATTGAAGATGAACTAAATCGTGAAAGTGACAGTGATGTCTTCACCGTTGTAATTAGCTATGCCATCATGTTTCTATATATTTCCCTAG
CCTTGGGGCACATGAAAAGCTGTCGCAGGCTTCTGGAGACTAATGAGCAAAAACTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAA
GAGCTTATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTGTTGGAAGAAGCAAACCGAGGCTCGTTTCCAGCAGAGA
TGGTGAATAAAATCTTTTCTAATATTTCATCAATAAATGCCTTCCATAGTAAATTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAAT
GGGAAACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTTAAGATGTATGGAGAATATGTGAAAGGATTTGATAATG
CAATGGAATTGGTTAAAAACATGACAGAACGTATTCCCCAGTTCAAATCAGTGGTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCT
TAACTTTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAGATGCTCCTTAAGGACTATCTAAGGAAATTGCCTCCTG
ATTCCCTGGACTGGAATGATGCTAAAAAATCACTTGAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGA
ACCTAAAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATTGTAAACCCTTCAAATGAACTAATAAAAGAAGGACAGA
TCCTCAAACTAGCTGCTCGGAACACTTCAGCACAAGAACGCTACCTTTTCTTATTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCA
GCTTGGTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATGAAAATTGTAGAGACTCAAAATGAAGAATATCCACATA
CTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAACTGCAGGCCAGTTCTGCGCAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAA
CCATCGATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAGGATAATGACATTCACTCAGAGGTTTCTACTGCTGAGC
TAGGGAAAAGAGCCCCAAGATGGATCCGAGATAATGAAGTGACAATGTGTATGAAATGTAAAGAACCTTTCAATGCACTGACACGAAGGA
GGCATCATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTACAAAGCTCAACTTGAATATGATGGTGGTAAATTGAGCA
AAGTTTGTAAAGACTGTTATCAAATCATAAGTGGATTCACAGACAGTGAAGAAAAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAG
AAGTATCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCAAAACCTTGGCAGAAAGCTTGGTGTGTGATCCCCAAGC
AAGACCCTCTTGTGCTGTACATGTATGGTGCCCCCCAGGACGTCAGAGCCCAGGCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAA
TGCCAAGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCCGTGCACAGCTTTGCTGCAGACAGTGAGGAACTGAAGC
AGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACAGGTGAGACACCAGGTGGTCCAAATGAGCATCCAGCCACCTTGGATGATCATCCTG
AACCTAAGAAAAAATCAGAATGCTGAACTCCTCCAGGACCAGCCATGGTGTGGAGGTCTCAGGACTTACAGCTCAAGACATTCCCAGCTC
TTCTTACACATCTGCTAGCACTTTATGTTGAAAAATATAGGCCCATAAATGCATCTTTTGAGGACTATTTTCCTATGTTTATGTACTCTT

>59853_59853_5_NPC1-FGD4_NPC1_chr18_21124924_ENST00000412552_FGD4_chr12_32751431_ENST00000531134_length(amino acids)=900AA_BP=334
MDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGTAWLLTSTFPSSPVLPGEASCCDPVSAAFEGCLRR
LFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGA
DVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCV
RFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLETNEQKLHKIANELLLTERAYVNRLD
LLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPELEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNM
TERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDYLRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEI
YEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLLYCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTFQVSGKE
RTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHSEVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACG
YVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGILEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYM
YGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHSFAADSEELKQKWLKVILLAVTGETPGGPNEHPATLDDHPEPKKKSEC

--------------------------------------------------------------
>59853_59853_6_NPC1-FGD4_NPC1_chr18_21124924_ENST00000412552_FGD4_chr12_32751431_ENST00000534526_length(transcript)=2726nt_BP=1025nt
CTGCTCCCTGGACGATCCTTGGCTTGGACGCCATGTATGTCATCATGTGGATCACCTACATGGCGTTTTTGCTTGTGTTTTTTGGAGCAT
TTTTTGCAGTGTGGTGCTACAGAAAACGGTATTTTGTCTCCGAGTACACTCCCATCGATAGCAATATAGCTTTTTCTGTTAATGCAAGTG
ACAAAGGAACGGCTTGGCTCTTAACCTCCACCTTCCCTTCCTCTCCCGTTCTTCCAGGAGAGGCGTCCTGCTGTGACCCTGTCAGCGCAG
CATTTGAGGGCTGCTTGAGGCGGCTGTTCACACGCTGGGGGTCTTTCTGCGTCCGAAACCCTGGCTGTGTCATTTTCTTCTCGCTGGTCT
TCATTACTGCGTGTTCGTCAGGCCTGGTGTTTGTCCGGGTCACAACCAATCCAGTTGACCTCTGGTCAGCCCCCAGCAGCCAGGCTCGCC
TGGAAAAAGAGTACTTTGACCAGCACTTTGGGCCTTTCTTCCGGACGGAGCAGCTCATCATCCGGGCCCCTCTCACTGACAAACACATTT
ACCAGCCATACCCTTCGGGAGCTGATGTACCCTTTGGACCTCCGCTTGACATACAGATACTGCACCAGGTTCTTGACTTACAAATAGCCA
TCGAAAACATTACTGCCTCTTATGACAATGAGACTGTGACACTTCAAGACATCTGCTTGGCCCCTCTTTCACCGTATAACACGAACTGCA
CCATTTTGAGTGTGTTAAATTACTTCCAGAACAGCCATTCCGTGCTGGACCACAAGAAAGGGGACGACTTCTTTGTGTATGCCGATTACC
ACACGCACTTTCTGTACTGCGTACGGTTTATTAATTTTGTGAAAAACTACAAGAATCCCAATCTGACCATTTCCTTCACTGCTGAACGAA
GTATTGAAGATGAACTAAATCGTGAAAGTGACAGTGATGTCTTCACCGTTGTAATTAGCTATGCCATCATGTTTCTATATATTTCCCTAG
CCTTGGGGCACATGAAAAGCTGTCGCAGGCTTCTGGAGACTAATGAGCAAAAACTTCACAAAATAGCCAATGAACTTTTGCTTACTGAAA
GAGCTTATGTCAACCGACTTGACCTCTTAGATCAGGTATTTTATTGCAAACTGTTGGAAGAAGCAAACCGAGGCTCGTTTCCAGCAGAGA
TGGTGAATAAAATCTTTTCTAATATTTCATCAATAAATGCCTTCCATAGTAAATTCCTCTTGCCAGAGCTGGAGAAACGAATGCAAGAAT
GGGAAACTACTCCTAGAATTGGAGACATCCTTCAGAAATTGGCACCATTCCTTAAGATGTATGGAGAATATGTGAAAGGATTTGATAATG
CAATGGAATTGGTTAAAAACATGACAGAACGTATTCCCCAGTTCAAATCAGTGGTTGAAGAAATTCAGAAACAGAAAATCTGTGGGAGCT
TAACTTTGCAGCATCACATGCTAGAACCTGTTCAGCGGATTCCCCGGTATGAGATGCTCCTTAAGGACTATCTAAGGAAATTGCCTCCTG
ATTCCCTGGACTGGAATGATGCTAAAAAATCACTTGAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGGAAAATGGAGA
ACCTAAAGAAACTCTTAGAGATTTATGAAATGTTGGGAGAAGAAGAAGACATTGTAAACCCTTCAAATGAACTAATAAAAGAAGGACAGA
TCCTCAAACTAGCTGCTCGGAACACTTCAGCACAAGAACGCTACCTTTTCTTATTCAACAACATGTTGCTGTACTGTGTGCCCAAATTCA
GCTTGGTAGGCTCTAAATTCACAGTTCGAACCAGGGTTGGCATTGATGGAATGAAAATTGTAGAGACTCAAAATGAAGAATATCCACATA
CTTTCCAGGTGTCTGGGAAAGAGAGAACACTGGAACTGCAGGCCAGTTCTGCGCAAGACAAAGAAGAATGGATCAAGGCCCTTCAAGAAA
CCATCGATGCTTTTCATCAAAGGCATGAAACCTTCAGAAATGCAATTGCAAAGGATAATGACATTCACTCAGAGGTTTCTACTGCTGAGC
TAGGGAAAAGAGCCCCAAGATGGATCCGAGATAATGAAGTGACAATGTGTATGAAATGTAAAGAACCTTTCAATGCACTGACACGAAGGA
GGCATCATTGTCGAGCATGTGGATATGTGGTTTGTTGGAAATGCTCCGACTACAAAGCTCAACTTGAATATGATGGTGGTAAATTGAGCA
AAGTTTGTAAAGACTGTTATCAAATCATAAGTGGATTCACAGACAGTGAAGAAAAGAAAAGAAAAGGAATTTTAGAGATTGAATCAGCAG
AAGTATCTGGAAACAGTGTGGTGTGCAGCTTTCTTCAGTATATGGAGAAGTCAAAACCTTGGCAGAAAGCTTGGTGTGTGATCCCCAAGC
AAGACCCTCTTGTGCTGTACATGTATGGTGCCCCCCAGGACGTCAGAGCCCAGGCCACCATTCCACTTCTGGGCTATGTGGTGGATGAAA
TGCCAAGGAGCGCAGACCTGCCACACAGTTTCAAACTGACCCAGTCTAAGTCCGTGCACAGCTTTGCTGCAGACAGTGAGGAACTGAAGC
AGAAGTGGCTGAAAGTCATCCTTTTAGCTGTCACAGGTGAGACACCAGGTGGTCCAAATGAGCATCCAGCCACCTTGGATGATCATCCTG

>59853_59853_6_NPC1-FGD4_NPC1_chr18_21124924_ENST00000412552_FGD4_chr12_32751431_ENST00000534526_length(amino acids)=901AA_BP=334
MDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGTAWLLTSTFPSSPVLPGEASCCDPVSAAFEGCLRR
LFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGA
DVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCV
RFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLETNEQKLHKIANELLLTERAYVNRLD
LLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPELEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNM
TERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDYLRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEI
YEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLLYCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTFQVSGKE
RTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHSEVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACG
YVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGILEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYM
YGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHSFAADSEELKQKWLKVILLAVTGETPGGPNEHPATLDDHPEPKKKSEC

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Fusion Gene PPI Analysis for NPC1-FGD4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for NPC1-FGD4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for NPC1-FGD4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource