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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:NRBF2-EYA2 (FusionGDB2 ID:60197) |
Fusion Gene Summary for NRBF2-EYA2 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: NRBF2-EYA2 | Fusion gene ID: 60197 | Hgene | Tgene | Gene symbol | NRBF2 | EYA2 | Gene ID | 29982 | 2139 |
| Gene name | nuclear receptor binding factor 2 | EYA transcriptional coactivator and phosphatase 2 | |
| Synonyms | COPR|COPR1|COPR2|NRBF-2 | EAB1 | |
| Cytomap | 10q21.3 | 20q13.12 | |
| Type of gene | protein-coding | protein-coding | |
| Description | nuclear receptor-binding factor 2comodulator of PPAR and RXR 1comodulator of PPAR and RXR 2 | eyes absent homolog 2 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q96F24 | O00167 | |
| Ensembl transtripts involved in fusion gene | ENST00000277746, ENST00000435510, | ENST00000317304, ENST00000497428, ENST00000327619, ENST00000357410, | |
| Fusion gene scores | * DoF score | 9 X 6 X 5=270 | 15 X 15 X 10=2250 |
| # samples | 10 | 30 | |
| ** MAII score | log2(10/270*10)=-1.43295940727611 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(30/2250*10)=-2.90689059560852 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: NRBF2 [Title/Abstract] AND EYA2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | NRBF2(64893260)-EYA2(45771698), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | NRBF2-EYA2 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | EYA2 | GO:0016576 | histone dephosphorylation | 19351884 |
| Fusion gene breakpoints across NRBF2 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across EYA2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | OV | TCGA-24-1842-01A | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
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Fusion Gene ORF analysis for NRBF2-EYA2 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| 5CDS-intron | ENST00000277746 | ENST00000317304 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| 5CDS-intron | ENST00000277746 | ENST00000497428 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| 5CDS-intron | ENST00000435510 | ENST00000317304 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| 5CDS-intron | ENST00000435510 | ENST00000497428 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| Frame-shift | ENST00000435510 | ENST00000327619 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| Frame-shift | ENST00000435510 | ENST00000357410 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| In-frame | ENST00000277746 | ENST00000327619 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| In-frame | ENST00000277746 | ENST00000357410 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000277746 | NRBF2 | chr10 | 64893260 | + | ENST00000357410 | EYA2 | chr20 | 45771698 | + | 1414 | 211 | 64 | 702 | 212 |
| ENST00000277746 | NRBF2 | chr10 | 64893260 | + | ENST00000327619 | EYA2 | chr20 | 45771698 | + | 1651 | 211 | 64 | 939 | 291 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000277746 | ENST00000357410 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + | 0.002351237 | 0.9976488 |
| ENST00000277746 | ENST00000327619 | NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771698 | + | 0.001592662 | 0.9984073 |
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Fusion Genomic Features for NRBF2-EYA2 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771697 | + | 5.38E-08 | 1 |
| NRBF2 | chr10 | 64893260 | + | EYA2 | chr20 | 45771697 | + | 5.38E-08 | 1 |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
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| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for NRBF2-EYA2 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:64893260/chr20:45771698) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| NRBF2 | EYA2 |
| FUNCTION: May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro). {ECO:0000269|PubMed:15610520}.; FUNCTION: Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy (PubMed:24785657). Stabilzes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3 (By similarity). Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1 (PubMed:25086043). May be involved in autophagosome biogenesis (PubMed:25086043). May play a role in neural progenitor cell survival during differentiation (By similarity). {ECO:0000250|UniProtKB:Q8VCQ3, ECO:0000269|PubMed:24785657, ECO:0000269|PubMed:25086043}. | FUNCTION: Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:12500905, PubMed:23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed:19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed:12500905). {ECO:0000269|PubMed:12500905, ECO:0000269|PubMed:19351884, ECO:0000269|PubMed:21706047, ECO:0000269|PubMed:23435380}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | NRBF2 | chr10:64893260 | chr20:45771698 | ENST00000277746 | + | 1 | 4 | 168_209 | 10 | 288.0 | Coiled coil | Ontology_term=ECO:0000255 |
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Fusion Gene Sequence for NRBF2-EYA2 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >60197_60197_1_NRBF2-EYA2_NRBF2_chr10_64893260_ENST00000277746_EYA2_chr20_45771698_ENST00000327619_length(transcript)=1651nt_BP=211nt CGCGGCGCGGCGCCGGAGGAGGAAGTGGTGAGGTTGTTGCTCCTTCAGCGCCTATCGCTGGCTCTTGGGGCGCAGAGAGGGGCCGCAGTC TCCGCGGCTGCGTCGAGCTCCCTTGCAGTCCCCTCCATGTTCCCCGGCGCCACTACTCCCCTTCCTAAGGCCGCCGCTTACCCCGGGGTC TATGGAAGTAATGGAAGGACCCCTCAACCTGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGC AGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAG CACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTG GATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTGGGTTGATAGGCACTCCCAA AAGGGAGACCTGGCTACAGCTCCGAGCTGAGCTGGAAGCTCTCACAGACCTCTGGCTGACCCACTCCCTGAAGGCACTAAACCTCATCAA CTCCCGGCCCAACTGTGTCAATGTGCTGGTCACCACCACTCAACTAATTCCTGCCCTGGCCAAAGTCCTGCTATATGGCCTGGGGTCTGT GTTTCCTATTGAGAACATCTACAGTGCAACCAAGACAGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCGGCAGAAAAGCTGT CTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCT GGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTCAGACCCCCTCGCC TTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCAT CTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTTTGGGGAAAAGGGC TGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATG GGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGT TGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGAC AATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGACCTACTTGACGTCA TGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAG >60197_60197_1_NRBF2-EYA2_NRBF2_chr10_64893260_ENST00000277746_EYA2_chr20_45771698_ENST00000327619_length(amino acids)=291AA_BP=49 MGRREGPQSPRLRRAPLQSPPCSPAPLLPFLRPPLTPGSMEVMEGPLNLDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVS SDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQLRAELEALTDLWLTH SLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAVYVVIGDGVEEEQGAKKHNMPF -------------------------------------------------------------- >60197_60197_2_NRBF2-EYA2_NRBF2_chr10_64893260_ENST00000277746_EYA2_chr20_45771698_ENST00000357410_length(transcript)=1414nt_BP=211nt CGCGGCGCGGCGCCGGAGGAGGAAGTGGTGAGGTTGTTGCTCCTTCAGCGCCTATCGCTGGCTCTTGGGGCGCAGAGAGGGGCCGCAGTC TCCGCGGCTGCGTCGAGCTCCCTTGCAGTCCCCTCCATGTTCCCCGGCGCCACTACTCCCCTTCCTAAGGCCGCCGCTTACCCCGGGGTC TATGGAAGTAATGGAAGGACCCCTCAACCTGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCTTCAACCTTGC AGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACGTTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAG CACATACAACTTCTCCGCTGACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCGGCGTGGACTG GATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATACCTACAAGAACAACGTTGGTGGGAAGGAGAGCTGCTTCGA GAGGATAATGCAGAGATTCGGCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAAAGCACAACAT GCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACGCCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCT CCACCTGCCATCTCACCCTCAGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACACCAGGAAGGGG CCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATCAGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATT GGCTTCGGAGTATTTGACTTTGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAAGGAATTGCTG ATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCTTTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGC TCTTGTACCTGTGTGGCTGGGTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTTCTAATGTAAG CACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACATTAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGC TTATTTTTGTTTTTAAAGACCTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGAGGGGGATGCT >60197_60197_2_NRBF2-EYA2_NRBF2_chr10_64893260_ENST00000277746_EYA2_chr20_45771698_ENST00000357410_length(amino acids)=212AA_BP=49 MGRREGPQSPRLRRAPLQSPPCSPAPLLPFLRPPLTPGSMEVMEGPLNLDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVS SDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFGRKAVYVVIGDGVEE -------------------------------------------------------------- |
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Fusion Gene PPI Analysis for NRBF2-EYA2 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for NRBF2-EYA2 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for NRBF2-EYA2 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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