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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:PC-ACTN4 (FusionGDB2 ID:63059)

Fusion Gene Summary for PC-ACTN4

Fusion gene summary

Fusion gene information	Fusion gene name: PC-ACTN4
	Fusion gene ID: 63059
		Hgene	Tgene
	Gene symbol	PC	ACTN4
	Gene ID	5624	81
	Gene name	protein C, inactivator of coagulation factors Va and VIIIa	actinin alpha 4
	Synonyms	APC\|PC\|PROC1\|THPH3\|THPH4	ACTININ-4\|FSGS\|FSGS1
	Cytomap	2q14.3	19q13.2
	Type of gene	protein-coding	protein-coding
	Description	vitamin K-dependent protein CProtein C-Nagoyaactivated protein Canticoagulant protein Cautoprothrombin IIAblood coagulation factor XIVprepro-protein Ctype I protein C	alpha-actinin-4focal segmental glomerulosclerosis 1non-muscle alpha-actinin 4
	Modification date	20200313	20200327
	UniProtAcc	.	O43707
	Ensembl transtripts involved in fusion gene	ENST00000355677, ENST00000393955, ENST00000393958, ENST00000393960, ENST00000524491, ENST00000528224, ENST00000529047,	ENST00000390009, ENST00000497637, ENST00000252699, ENST00000424234,
Fusion gene scores	* DoF score	19 X 8 X 8=1216	27 X 38 X 12=12312
	# samples	18	48
	** MAII score	log2(18/1216*10)=-2.75607441711391 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(48/12312*10)=-4.68088692071969 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: PC [Title/Abstract] AND ACTN4 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	PC(66631245)-ACTN4(39191240), # samples:3
Anticipated loss of major functional domain due to fusion event.	PC-ACTN4 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF. PC-ACTN4 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. PC-ACTN4 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	ACTN4	GO:0033209	tumor necrosis factor-mediated signaling pathway	25411248
Tgene	ACTN4	GO:0035357	peroxisome proliferator activated receptor signaling pathway	22351778
Tgene	ACTN4	GO:0048384	retinoic acid receptor signaling pathway	22351778
Tgene	ACTN4	GO:0051272	positive regulation of cellular component movement	9508771

Fusion gene breakpoints across PC (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ACTN4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LIHC	TCGA-BC-A10T-01A	PC	chr11	66631245	-	ACTN4	chr19	39191240	+

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Fusion Gene ORF analysis for PC-ACTN4

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-intron	ENST00000355677	ENST00000390009	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000355677	ENST00000497637	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000393955	ENST00000390009	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000393955	ENST00000497637	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000393958	ENST00000390009	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000393958	ENST00000497637	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000393960	ENST00000390009	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000393960	ENST00000497637	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000524491	ENST00000390009	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
5CDS-intron	ENST00000524491	ENST00000497637	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
Frame-shift	ENST00000355677	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
Frame-shift	ENST00000355677	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
Frame-shift	ENST00000393958	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
Frame-shift	ENST00000393958	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
Frame-shift	ENST00000524491	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
Frame-shift	ENST00000524491	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
In-frame	ENST00000393955	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
In-frame	ENST00000393955	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
In-frame	ENST00000393960	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
In-frame	ENST00000393960	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-3CDS	ENST00000528224	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-3CDS	ENST00000528224	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-3CDS	ENST00000529047	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-3CDS	ENST00000529047	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-intron	ENST00000528224	ENST00000390009	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-intron	ENST00000528224	ENST00000497637	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-intron	ENST00000529047	ENST00000390009	PC	chr11	66631245	-	ACTN4	chr19	39191240	+
intron-intron	ENST00000529047	ENST00000497637	PC	chr11	66631245	-	ACTN4	chr19	39191240	+

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000393960	PC	chr11	66631245	-	ENST00000252699	ACTN4	chr19	39191240	+	6375	1650	210	4223	1337
ENST00000393960	PC	chr11	66631245	-	ENST00000424234	ACTN4	chr19	39191240	+	3054	1650	210	3053	947
ENST00000393955	PC	chr11	66631245	-	ENST00000252699	ACTN4	chr19	39191240	+	6174	1449	54	4022	1322
ENST00000393955	PC	chr11	66631245	-	ENST00000424234	ACTN4	chr19	39191240	+	2853	1449	54	2852	932

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000393960	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+	0.004021811	0.9959781
ENST00000393960	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+	0.008098478	0.99190146
ENST00000393955	ENST00000252699	PC	chr11	66631245	-	ACTN4	chr19	39191240	+	0.003758695	0.99624133
ENST00000393955	ENST00000424234	PC	chr11	66631245	-	ACTN4	chr19	39191240	+	0.009363133	0.9906369

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Fusion Genomic Features for PC-ACTN4

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	1-p	p (fusion gene breakpoint)
PC	chr11	66631244	-	ACTN4	chr19	39191239	+	3.37E-08	1
PC	chr11	66631244	-	ACTN4	chr19	39191239	+	3.37E-08	1

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for PC-ACTN4

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:66631245/chr19:39191240)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	ACTN4 O43707
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.	FUNCTION: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). {ECO:0000250\|UniProtKB:P57780, ECO:0000269\|PubMed:15772161, ECO:0000269\|PubMed:22351778, ECO:0000305\|PubMed:9508771}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

chr11:66631245

chr19:39191240

ENST00000393955

156_353

456

1179.0

Domain

ATP-grasp

Hgene

chr11:66631245

chr19:39191240

ENST00000393958

156_353

456

1179.0

Domain

ATP-grasp

Hgene

chr11:66631245

chr19:39191240

ENST00000393960

156_353

456

1179.0

Domain

ATP-grasp

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

778_789

912.0

Calcium binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

819_830

912.0

Calcium binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

778_789

693.0

Calcium binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

819_830

693.0

Calcium binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

19_26

693.0

Compositional bias

Poly-Gly

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

163_269

912.0

Domain

Calponin-homology (CH) 2

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

765_800

912.0

Domain

EF-hand 1

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

806_841

912.0

Domain

EF-hand 2

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

163_269

693.0

Domain

Calponin-homology (CH) 2

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

50_154

693.0

Domain

Calponin-homology (CH) 1

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

765_800

693.0

Domain

EF-hand 1

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

806_841

693.0

Domain

EF-hand 2

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

84_88

912.0

Motif

LXXLL motif

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

84_88

693.0

Motif

LXXLL motif

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

177_192

912.0

Region

Polyphosphoinositide (PIP2)-binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

177_192

693.0

Region

Polyphosphoinositide (PIP2)-binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

1_269

693.0

Region

Note=Actin-binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

293_403

912.0

Repeat

Spectrin 1

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

413_518

912.0

Repeat

Spectrin 2

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

528_639

912.0

Repeat

Spectrin 3

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

649_752

912.0

Repeat

Spectrin 4

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

293_403

693.0

Repeat

Spectrin 1

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

413_518

693.0

Repeat

Spectrin 2

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

528_639

693.0

Repeat

Spectrin 3

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000390009

649_752

693.0

Repeat

Spectrin 4

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

chr11:66631245

chr19:39191240

ENST00000393955

1109_1178

456

1179.0

Domain

Biotinyl-binding

Hgene

chr11:66631245

chr19:39191240

ENST00000393955

36_486

456

1179.0

Domain

Note=Biotin carboxylation

Hgene

chr11:66631245

chr19:39191240

ENST00000393955

563_832

456

1179.0

Domain

Pyruvate carboxyltransferase

Hgene

chr11:66631245

chr19:39191240

ENST00000393958

1109_1178

456

1179.0

Domain

Biotinyl-binding

Hgene

chr11:66631245

chr19:39191240

ENST00000393958

36_486

456

1179.0

Domain

Note=Biotin carboxylation

Hgene

chr11:66631245

chr19:39191240

ENST00000393958

563_832

456

1179.0

Domain

Pyruvate carboxyltransferase

Hgene

chr11:66631245

chr19:39191240

ENST00000393960

1109_1178

456

1179.0

Domain

Biotinyl-binding

Hgene

chr11:66631245

chr19:39191240

ENST00000393960

36_486

456

1179.0

Domain

Note=Biotin carboxylation

Hgene

chr11:66631245

chr19:39191240

ENST00000393960

563_832

456

1179.0

Domain

Pyruvate carboxyltransferase

Hgene

chr11:66631245

chr19:39191240

ENST00000393955

571_575

456

1179.0

Region

Note=Substrate binding

Hgene

chr11:66631245

chr19:39191240

ENST00000393958

571_575

456

1179.0

Region

Note=Substrate binding

Hgene

chr11:66631245

chr19:39191240

ENST00000393960

571_575

456

1179.0

Region

Note=Substrate binding

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

19_26

912.0

Compositional bias

Poly-Gly

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

50_154

912.0

Domain

Calponin-homology (CH) 1

Tgene

ACTN4

chr11:66631245

chr19:39191240

ENST00000252699

1_269

912.0

Region

Note=Actin-binding

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Fusion Gene Sequence for PC-ACTN4

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>63059_63059_1_PC-ACTN4_PC_chr11_66631245_ENST00000393955_ACTN4_chr19_39191240_ENST00000252699_length(transcript)=6174nt_BP=1449nt
CAGGCAGCAGGCCTCAGGCCCAGACTTGGGTGTTACAGGAACAGTGTGGCCTCTCTGGAACTCTGCAGACCAACTGCCGTGATGCTGAAG
TTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGGGAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCCCCAAATGTCCGGCGCCTGGAG
TATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGTTCCGGGCCTGCACGGAGCTGGGCATCCGCACC
GTAGCCATCTACTCTGAGCAGGACACGGGCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCGTG
CAGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGCACCCTGGCTACGGGTTCCTCTCTGAG
CGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAGGGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGGTG
GAGGCCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCCCCATCACGTCCCTGCATGAGGCCCACGAGTTC
TCCAACACCTACGGCTTCCCCATCATCTTCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTACGAGGAGCTG
GAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGTTTGTGGAGAAGTTCATCGAGAAGCCACGG
CACATCGAGGTGCAGATCTTGGGGGACCAGTATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGGCGGCACCAGAAG
GTGGTCGAGATTGCCCCCGCCGCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCAGCGACTCTGTGAAACTCGCTAAACAGGTGGGC
TACGAGAACGCAGGCACCGTGGAGTTCCTGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAACTCCCGCCTGCAGGTGGAGCAC
ACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCACGTGGCTGAGGGCAGGAGCCTACCCGACCTGGGCCTGCGG
CAGGAGAACATCCGCATCAACGGGTGTGCCATCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGCTTCCAGCCGGACACCGGCCGC
ATTGAGGTGTTCCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCTTCCAAGGAGCCGTCATCTCGCCCCACTACGAC
TCCCTGCTGGTCAAAGTCATTGCCCACGGCAAAGACCACCCCACGGCCGCCACCAAGATGAGCAGGGCCCTTGCGGAGTTCCGCGTCCGA
GGTGTGAAGACCTTCACGGCATGGTGCAACTCCCACCTGCGGAAGGCAGGCACACAGATCGAGAACATTGATGAGGACTTCCGAGACGGG
CTCAAGCTCATGCTGCTCCTGGAGGTCATATCAGGGGAGCGGTTACCTAAGCCGGAGCGGGGGAAGATGAGAGTGCACAAAATCAACAAT
GTGAACAAAGCGCTGGACTTTATTGCCAGCAAAGGCGTCAAGCTGGTCTCCATCGGGGCAGAAGAGATTGTGGACGGCAACGCAAAGATG
ACCCTGGGAATGATCTGGACCATCATCCTTAGGTTCGCCATCCAGGACATCTCCGTGGAAGAGACCTCGGCCAAGGAAGGGCTCCTTCTC
TGGTGCCAGAGAAAGACAGCCCCGTATAAGAACGTCAATGTGCAGAACTTCCACATCAGCTGGAAGGATGGTCTTGCCTTCAATGCCCTG
ATCCACCGGCACAGACCAGAGCTGATTGAGTATGACAAGCTGAGGAAGGACGACCCTGTCACCAACCTGAACAATGCCTTCGAAGTGGCT
GAGAAATACCTCGACATCCCCAAGATGCTGGATGCAGAGGACATCGTGAACACGGCCCGGCCCGACGAGAAGGCCATAATGACCTATGTG
TCCAGCTTCTACCATGCCTTTTCAGGAGCGCAGAAGGCTGAAACTGCCGCCAACCGGATCTGTAAGGTGCTGGCTGTCAACCAAGAGAAC
GAGCACCTGATGGAGGACTACGAGAAGCTGGCCAGCGACCTCCTGGAGTGGATCCGGCGCACCATCCCCTGGCTGGAGGACCGTGTGCCC
CAAAAGACTATCCAGGAGATGCAGCAGAAGCTGGAGGACTTCCGCGACTACCGGCGTGTGCACAAGCCGCCCAAGGTGCAGGAGAAGTGC
CAGCTGGAGATCAACTTCAACACGCTGCAGACCAAGCTGCGCCTCAGCAACCGGCCCGCCTTCATGCCCTCCGAGGGCAAGATGGTCTCG
GACATCAACAATGGCTGGCAGCACTTGGAGCAGGCTGAGAAGGGCTACGAGGAGTGGCTGCTGAATGAGATCCGCAGGCTGGAGCGGCTC
GACCACCTGGCAGAGAAGTTCCGGCAGAAGGCCTCCATCCACGAGGCCTGGACTGACGGGAAGGAAGCCATGCTGAAGCACCGGGACTAC
GAGACGGCCACACTATCGGACATCAAAGCCCTCATTCGCAAGCACGAGGCCTTCGAGAGCGACCTGGCTGCGCACCAGGACCGCGTGGAG
CAGATCGCCGCCATTGCCCAGGAGCTCAACGAGCTGGATTACTACGACTCCCACAATGTCAACACCCGGTGCCAGAAGATCTGTGACCAG
TGGGACGCCCTCGGCTCTCTGACACATAGTCGCAGGGAAGCCCTGGAGAAAACAGAGAAGCAGCTGGAGGCCATCGACCAGCTGCACCTG
GAATACGCCAAGCGCGCGGCCCCCTTCAACAACTGGATGGAGAGCGCCATGGAGGACCTCCAGGACATGTTCATCGTCCATACCATCGAG
GAGATTGAGGGCCTGATCTCAGCCCATGACCAGTTCAAGTCCACCCTGCCGGACGCCGATAGGGAGCGCGAGGCCATCCTGGCCATCCAC
AAGGAGGCCCAGAGGATCGCTGAGAGCAACCACATCAAGCTGTCGGGCAGCAACCCCTACACCACCGTCACCCCGCAAATCATCAACTCC
AAGTGGGAGAAGGTGCAGCAGCTGGTGCCAAAACGGGACCATGCCCTCCTGGAGGAGCAGAGCAAGCAGCAGTCCAACGAGCACCTGCGC
CGCCAGTTCGCCAGCCAGGCCAATGTTGTGGGGCCCTGGATCCAGACCAAGATGGAGGAGATCGGGCGCATCTCCATTGAGATGAACGGG
ACCCTGGAGGACCAGCTGAGCCACCTGAAGCAGTATGAACGCAGCATCGTGGACTACAAGCCCAACCTGGACCTGCTGGAGCAGCAGCAC
CAGCTCATCCAGGAGGCCCTCATCTTCGACAACAAGCACACCAACTATACCATGGAGCACATCCGCGTGGGCTGGGAGCAGCTGCTCACC
ACCATTGCCCGCACCATCAACGAGGTGGAGAACCAGATCCTCACCCGCGACGCCAAGGGCATCAGCCAGGAGCAGATGCAGGAGTTCCGG
GCGTCCTTCAACCACTTCGACAAGGATCATGGCGGGGCGCTGGGGCCCGAGGAGTTCAAGGCCTGCCTCATCAGCCTGGGCTACGACGTG
GAGAACGACCGGCAGGGTGAGGCCGAGTTCAACCGCATCATGAGCCTGGTCGACCCCAACCATAGCGGCCTTGTGACCTTCCAAGCCTTC
ATCGACTTCATGTCGCGGGAGACCACCGACACGGACACGGCTGACCAGGTCATCGCTTCCTTCAAGGTCTTAGCAGGGGACAAGAACTTC
ATCACAGCTGAGGAGCTGCGGAGAGAGCTGCCCCCCGACCAGGCCGAGTACTGCATCGCCCGCATGGCGCCATACCAGGGCCCTGACGCC
GTGCCCGGTGCCCTCGACTACAAGTCCTTCTCCACGGCCTTGTATGGCGAGAGCGACCTGTGAGGCCCCAGAGACCTGACCCAACACCCC
CGACGGCCTCCAGGAGGGGCCTGGGCAGCCCCACAGTCCCATTCCTCCACTCTGTATCTATGCAAAGCACTCTCTGCAGTCCTCCGGGGT
GGGTGGGTGGGCAGGGAGGGGCTGGGGCAGGCTCTCTCCTCTCTCTCTTTGTGGGTTGGCCAGGAGGTTCCCCCGACCAGGTTGGGGAGA
CTTGGGGCCAGCGCTTCTGGTCTGGTAAATATGTATGATGTGTTGTGCTTTTTTAACCAAGGAGGGGCCAGTGGATTCCCACAGCACAAC
CGGTCCCTTCCATGCCCTGGGATGCCTCACCACACCCAGGTCTCTTCCTTTGCTCTGAGGTCCCTTCAAGGCCTCCCCAATCCAGGCCAA
AGCCCCATGTGCCTTGTCCAGGAACTGCCTGGGCCATGCGAGGGGCCAGCAGAGGGCGCCACCACCACCTGACGGCTGGGGACCCACCCA
GCCCCTCTCCCCTCTCTGCTCCAGACTCACTTGCCATTGCCAGGAGATGGCCCCAACAAGCACCCCGCTTTTGCAGCAGAGGAGCTGAGT
TGGCAGACCGGGCCCCCCTGAACCGCACCCCATCCCACCAGCCCCGGCCTTGCTTTGTCTGGCCTCACGTGTCTCAGATTTTCTAAGAAC
CAAAAAAAAAAAAGGAAAAAAAACACAAAACAACAAAAACCAAAAAAAAAAAAAATCACAAAAACAAAAAAACTATAAAAAAGAAAGAAT
TAAAAACTTTCAGAGAATTACTATTTACTTTATTAACTTACGGATTTATTATATAAATATATATTCACCTAGCAACATATCTCTGCCGTC
TCTCCTGCTCTCATAATGAAGACATAGCCGATTCTCTGCCCGGGCCCCTTGCTGATGCTCCTCCGGGTCTGCGTCGGGCGTGGGTCTCTG
GGGACCCTCCAGAGGTGGAGGTGGGCTGATGGCCTGGCTGCCTGGTGGTTGATGGTTTTGCTCCCCCTACCTTTTTTTTTTGAGTTTATT
CTGATTGATTTTTTTTCTTGGTTTCTGGATAAACCACCCTCTGGGGACAGGATAATAAAACATGTAATATTTTTAAGAAGGATTCCTGCA
GCATCATCTTTTTTTATTTCTCCTGTGTCTGTCCTCCACCTTCTAGGAGAGCCAGGGCAGAGCTAGCACTGTCTTAAGCTGTCAACGTGG
ACTAGCTCGTGTCATCTGCTCGAGAAGGGCTGTCGCTGTTCTTGTTTCTGAGTGAGGAGTACGCAGGCCAGAGTGGTCACCCGGCCGTGA
GCAGTGAGGGCCAGAGACTAGCCCCAGACAGGTGGATGCCAGAGAGAGTGGCACCCATGCCAGGCAAGGCCTAGGGAGGTGGTCTTGCTC
AGCAACCCTGCCCTGAGCAGCAGGTGCGCCCATCCGGAGATCCTAGGAGAAGGTGGCCACCTCCATCCACTAAGGAAGAGAAGGAAGACA
GTGGCTTGAGGCAGGGAGCTCGCAGGACAGAGCCTGAGCCACCCTGTCCCTCCCACCTGGCTCACCTGTCTGTGGGTCAGGCAGATGACC
CCCTCACCCCCATCCAGGTGCTGGCTGCAGTGGCCTGTGCAGAGAGGGGCAGGGTGAGTGCCCACCAGTCCCCGTACCCCTTCCCCCCAT
GCCCCACCATGCCGGGGTGGTACTCACAGAAGATGCAGGTGAGGTGGGCCACACAGGACACGAACCGCTCGAAGTCCACACGCAGACGGC
TATCCCGGTAGCGGCTGGTGAGGGTCTGGGTCAGCTGGTTGTTCAGGTGGAAGCCTAGGGGGAGGCTGCTTCTGAGCCCAGTGGCCCACA
GGGAACCCACCTTGGCATTGCATCCCCACCCCACCTCCTCAGGGAGGACATGAATGCCACAGGGTGTCACACCCCAACTCTGCCCCATCC
CGGCAGGGTGAGTACAGGCTGATCCCTTCAATCCTCTGGGCCTGTTTCCTCATCCATCAAACGGACTAAGACAGCCCCGACCCCATAGGG
TGTGTGAAGACAGAACGCTCAGGACAGCGTCTGACACGTGACTCGATGTGTGGGTACTGTTACTCCTTAAATCCTGTGGGGCTTTCTCCT

>63059_63059_1_PC-ACTN4_PC_chr11_66631245_ENST00000393955_ACTN4_chr19_39191240_ENST00000252699_length(amino acids)=1322AA_BP=465
MELCRPTAVMLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKAD
EAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTD
APITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLY
ERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHV
AEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAAT
KMSRALAEFRVRGVKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSI
GAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDD
PVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWI
RRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEE
WLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSH
NVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPD
ADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKM
EEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDA
KGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVI

--------------------------------------------------------------
>63059_63059_2_PC-ACTN4_PC_chr11_66631245_ENST00000393955_ACTN4_chr19_39191240_ENST00000424234_length(transcript)=2853nt_BP=1449nt
CAGGCAGCAGGCCTCAGGCCCAGACTTGGGTGTTACAGGAACAGTGTGGCCTCTCTGGAACTCTGCAGACCAACTGCCGTGATGCTGAAG
TTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGGGAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCCCCAAATGTCCGGCGCCTGGAG
TATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGTTCCGGGCCTGCACGGAGCTGGGCATCCGCACC
GTAGCCATCTACTCTGAGCAGGACACGGGCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCGTG
CAGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGCACCCTGGCTACGGGTTCCTCTCTGAG
CGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAGGGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGGTG
GAGGCCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCCCCATCACGTCCCTGCATGAGGCCCACGAGTTC
TCCAACACCTACGGCTTCCCCATCATCTTCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTACGAGGAGCTG
GAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGTTTGTGGAGAAGTTCATCGAGAAGCCACGG
CACATCGAGGTGCAGATCTTGGGGGACCAGTATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGGCGGCACCAGAAG
GTGGTCGAGATTGCCCCCGCCGCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCAGCGACTCTGTGAAACTCGCTAAACAGGTGGGC
TACGAGAACGCAGGCACCGTGGAGTTCCTGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAACTCCCGCCTGCAGGTGGAGCAC
ACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCACGTGGCTGAGGGCAGGAGCCTACCCGACCTGGGCCTGCGG
CAGGAGAACATCCGCATCAACGGGTGTGCCATCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGCTTCCAGCCGGACACCGGCCGC
ATTGAGGTGTTCCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCTTCCAAGGAGCCGTCATCTCGCCCCACTACGAC
TCCCTGCTGGTCAAAGTCATTGCCCACGGCAAAGACCACCCCACGGCCGCCACCAAGATGAGCAGGGCCCTTGCGGAGTTCCGCGTCCGA
GGTGTGAAGACCTTCACGGCATGGTGCAACTCCCACCTGCGGAAGGCAGGCACACAGATCGAGAACATTGATGAGGACTTCCGAGACGGG
CTCAAGCTCATGCTGCTCCTGGAGGTTCACGAGCTGGATTACTACGACTCCCACAATGTCAACACCCGGTGCCAGAAGATCTGTGACCAG
TGGGACGCCCTCGGCTCTCTGACACATAGTCGCAGGGAAGCCCTGGAGAAAACAGAGAAGCAGCTGGAGGCCATCGACCAGCTGCACCTG
GAATACGCCAAGCGCGCGGCCCCCTTCAACAACTGGATGGAGAGCGCCATGGAGGACCTCCAGGACATGTTCATCGTCCATACCATCGAG
GAGATTGAGGGCCTGATCTCAGCCCATGACCAGTTCAAGTCCACCCTGCCGGACGCCGATAGGGAGCGCGAGGCCATCCTGGCCATCCAC
AAGGAGGCCCAGAGGATCGCTGAGAGCAACCACATCAAGCTGTCGGGCAGCAACCCCTACACCACCGTCACCCCGCAAATCATCAACTCC
AAGTGGGAGAAGGTGCAGCAGCTGGTGCCAAAACGGGACCATGCCCTCCTGGAGGAGCAGAGCAAGCAGCAGTCCAACGAGCACCTGCGC
CGCCAGTTCGCCAGCCAGGCCAATGTTGTGGGGCCCTGGATCCAGACCAAGATGGAGGAGATCGGGCGCATCTCCATTGAGATGAACGGG
ACCCTGGAGGACCAGCTGAGCCACCTGAAGCAGTATGAACGCAGCATCGTGGACTACAAGCCCAACCTGGACCTGCTGGAGCAGCAGCAC
CAGCTCATCCAGGAGGCCCTCATCTTCGACAACAAGCACACCAACTATACCATGGAGCACATCCGCGTGGGCTGGGAGCAGCTGCTCACC
ACCATTGCCCGCACCATCAACGAGGTGGAGAACCAGATCCTCACCCGCGACGCCAAGGGCATCAGCCAGGAGCAGATGCAGGAGTTCCGG
GCGTCCTTCAACCACTTCGACAAGGATCATGGCGGGGCGCTGGGGCCCGAGGAGTTCAAGGCCTGCCTCATCAGCCTGGGCTACGACGTG
GAGAACGACCGGCAGGGTGAGGCCGAGTTCAACCGCATCATGAGCCTGGTCGACCCCAACCATAGCGGCCTTGTGACCTTCCAAGCCTTC
ATCGACTTCATGTCGCGGGAGACCACCGACACGGACACGGCTGACCAGGTCATCGCTTCCTTCAAGGTCTTAGCAGGGGACAAGAACTTC
ATCACAGCTGAGGAGCTGCGGAGAGAGCTGCCCCCCGACCAGGCCGAGTACTGCATCGCCCGCATGGCGCCATACCAGGGCCCTGACGCC

>63059_63059_2_PC-ACTN4_PC_chr11_66631245_ENST00000393955_ACTN4_chr19_39191240_ENST00000424234_length(amino acids)=932AA_BP=465
MELCRPTAVMLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKAD
EAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTD
APITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLY
ERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHV
AEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAAT
KMSRALAEFRVRGVKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVHELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKT
EKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSN
PYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVD
YKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEE
FKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYC

--------------------------------------------------------------
>63059_63059_3_PC-ACTN4_PC_chr11_66631245_ENST00000393960_ACTN4_chr19_39191240_ENST00000252699_length(transcript)=6375nt_BP=1650nt
GTCAGTGGAGGCAGCAGCGGTAGAGGCGGCGGCGAGGACTGGCGACGGCGAGGAGCTGCCTTCTAGTCTCACCTGTGGACACTTTGCCAA
AGGAGTCGGGCCTGACGGTGGAAGATGGGAAATGGCCAAAAGAATAAACCATTTCCTCTTACTTACCGAATGAAAAGCTTGCTTCATCTT
GGCGTTCAAGACTCTCTAATATGATTCCACCTGTTGGGCTCTTTCTTCCATTTGCCTCCGCAGATAGTGTCTGCCTTCTGGAGAGCTGAC
CAAACACTAAGGATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGGGAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCC
CCAAATGTCCGGCGCCTGGAGTATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGTTCCGGGCCTGC
ACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAGGACACGGGCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATC
GGCCGCGGCCTGGCCCCCGTGCAGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGCACCCT
GGCTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAGGGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTC
CGCAAGATGGGAGACAAGGTGGAGGCCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCCCCATCACGTCC
CTGCATGAGGCCCACGAGTTCTCCAACACCTACGGCTTCCCCATCATCTTCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTG
GTGCACAGCTACGAGGAGCTGGAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGTTTGTGGAG
AAGTTCATCGAGAAGCCACGGCACATCGAGGTGCAGATCTTGGGGGACCAGTATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCC
ATCCAGCGGCGGCACCAGAAGGTGGTCGAGATTGCCCCCGCCGCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCAGCGACTCTGTG
AAACTCGCTAAACAGGTGGGCTACGAGAACGCAGGCACCGTGGAGTTCCTGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAAC
TCCCGCCTGCAGGTGGAGCACACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCACGTGGCTGAGGGCAGGAGC
CTACCCGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAACGGGTGTGCCATCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGC
TTCCAGCCGGACACCGGCCGCATTGAGGTGTTCCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCTTCCAAGGAGCC
GTCATCTCGCCCCACTACGACTCCCTGCTGGTCAAAGTCATTGCCCACGGCAAAGACCACCCCACGGCCGCCACCAAGATGAGCAGGGCC
CTTGCGGAGTTCCGCGTCCGAGGTGTGAAGACCTTCACGGCATGGTGCAACTCCCACCTGCGGAAGGCAGGCACACAGATCGAGAACATT
GATGAGGACTTCCGAGACGGGCTCAAGCTCATGCTGCTCCTGGAGGTCATATCAGGGGAGCGGTTACCTAAGCCGGAGCGGGGGAAGATG
AGAGTGCACAAAATCAACAATGTGAACAAAGCGCTGGACTTTATTGCCAGCAAAGGCGTCAAGCTGGTCTCCATCGGGGCAGAAGAGATT
GTGGACGGCAACGCAAAGATGACCCTGGGAATGATCTGGACCATCATCCTTAGGTTCGCCATCCAGGACATCTCCGTGGAAGAGACCTCG
GCCAAGGAAGGGCTCCTTCTCTGGTGCCAGAGAAAGACAGCCCCGTATAAGAACGTCAATGTGCAGAACTTCCACATCAGCTGGAAGGAT
GGTCTTGCCTTCAATGCCCTGATCCACCGGCACAGACCAGAGCTGATTGAGTATGACAAGCTGAGGAAGGACGACCCTGTCACCAACCTG
AACAATGCCTTCGAAGTGGCTGAGAAATACCTCGACATCCCCAAGATGCTGGATGCAGAGGACATCGTGAACACGGCCCGGCCCGACGAG
AAGGCCATAATGACCTATGTGTCCAGCTTCTACCATGCCTTTTCAGGAGCGCAGAAGGCTGAAACTGCCGCCAACCGGATCTGTAAGGTG
CTGGCTGTCAACCAAGAGAACGAGCACCTGATGGAGGACTACGAGAAGCTGGCCAGCGACCTCCTGGAGTGGATCCGGCGCACCATCCCC
TGGCTGGAGGACCGTGTGCCCCAAAAGACTATCCAGGAGATGCAGCAGAAGCTGGAGGACTTCCGCGACTACCGGCGTGTGCACAAGCCG
CCCAAGGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTCAACACGCTGCAGACCAAGCTGCGCCTCAGCAACCGGCCCGCCTTCATGCCC
TCCGAGGGCAAGATGGTCTCGGACATCAACAATGGCTGGCAGCACTTGGAGCAGGCTGAGAAGGGCTACGAGGAGTGGCTGCTGAATGAG
ATCCGCAGGCTGGAGCGGCTCGACCACCTGGCAGAGAAGTTCCGGCAGAAGGCCTCCATCCACGAGGCCTGGACTGACGGGAAGGAAGCC
ATGCTGAAGCACCGGGACTACGAGACGGCCACACTATCGGACATCAAAGCCCTCATTCGCAAGCACGAGGCCTTCGAGAGCGACCTGGCT
GCGCACCAGGACCGCGTGGAGCAGATCGCCGCCATTGCCCAGGAGCTCAACGAGCTGGATTACTACGACTCCCACAATGTCAACACCCGG
TGCCAGAAGATCTGTGACCAGTGGGACGCCCTCGGCTCTCTGACACATAGTCGCAGGGAAGCCCTGGAGAAAACAGAGAAGCAGCTGGAG
GCCATCGACCAGCTGCACCTGGAATACGCCAAGCGCGCGGCCCCCTTCAACAACTGGATGGAGAGCGCCATGGAGGACCTCCAGGACATG
TTCATCGTCCATACCATCGAGGAGATTGAGGGCCTGATCTCAGCCCATGACCAGTTCAAGTCCACCCTGCCGGACGCCGATAGGGAGCGC
GAGGCCATCCTGGCCATCCACAAGGAGGCCCAGAGGATCGCTGAGAGCAACCACATCAAGCTGTCGGGCAGCAACCCCTACACCACCGTC
ACCCCGCAAATCATCAACTCCAAGTGGGAGAAGGTGCAGCAGCTGGTGCCAAAACGGGACCATGCCCTCCTGGAGGAGCAGAGCAAGCAG
CAGTCCAACGAGCACCTGCGCCGCCAGTTCGCCAGCCAGGCCAATGTTGTGGGGCCCTGGATCCAGACCAAGATGGAGGAGATCGGGCGC
ATCTCCATTGAGATGAACGGGACCCTGGAGGACCAGCTGAGCCACCTGAAGCAGTATGAACGCAGCATCGTGGACTACAAGCCCAACCTG
GACCTGCTGGAGCAGCAGCACCAGCTCATCCAGGAGGCCCTCATCTTCGACAACAAGCACACCAACTATACCATGGAGCACATCCGCGTG
GGCTGGGAGCAGCTGCTCACCACCATTGCCCGCACCATCAACGAGGTGGAGAACCAGATCCTCACCCGCGACGCCAAGGGCATCAGCCAG
GAGCAGATGCAGGAGTTCCGGGCGTCCTTCAACCACTTCGACAAGGATCATGGCGGGGCGCTGGGGCCCGAGGAGTTCAAGGCCTGCCTC
ATCAGCCTGGGCTACGACGTGGAGAACGACCGGCAGGGTGAGGCCGAGTTCAACCGCATCATGAGCCTGGTCGACCCCAACCATAGCGGC
CTTGTGACCTTCCAAGCCTTCATCGACTTCATGTCGCGGGAGACCACCGACACGGACACGGCTGACCAGGTCATCGCTTCCTTCAAGGTC
TTAGCAGGGGACAAGAACTTCATCACAGCTGAGGAGCTGCGGAGAGAGCTGCCCCCCGACCAGGCCGAGTACTGCATCGCCCGCATGGCG
CCATACCAGGGCCCTGACGCCGTGCCCGGTGCCCTCGACTACAAGTCCTTCTCCACGGCCTTGTATGGCGAGAGCGACCTGTGAGGCCCC
AGAGACCTGACCCAACACCCCCGACGGCCTCCAGGAGGGGCCTGGGCAGCCCCACAGTCCCATTCCTCCACTCTGTATCTATGCAAAGCA
CTCTCTGCAGTCCTCCGGGGTGGGTGGGTGGGCAGGGAGGGGCTGGGGCAGGCTCTCTCCTCTCTCTCTTTGTGGGTTGGCCAGGAGGTT
CCCCCGACCAGGTTGGGGAGACTTGGGGCCAGCGCTTCTGGTCTGGTAAATATGTATGATGTGTTGTGCTTTTTTAACCAAGGAGGGGCC
AGTGGATTCCCACAGCACAACCGGTCCCTTCCATGCCCTGGGATGCCTCACCACACCCAGGTCTCTTCCTTTGCTCTGAGGTCCCTTCAA
GGCCTCCCCAATCCAGGCCAAAGCCCCATGTGCCTTGTCCAGGAACTGCCTGGGCCATGCGAGGGGCCAGCAGAGGGCGCCACCACCACC
TGACGGCTGGGGACCCACCCAGCCCCTCTCCCCTCTCTGCTCCAGACTCACTTGCCATTGCCAGGAGATGGCCCCAACAAGCACCCCGCT
TTTGCAGCAGAGGAGCTGAGTTGGCAGACCGGGCCCCCCTGAACCGCACCCCATCCCACCAGCCCCGGCCTTGCTTTGTCTGGCCTCACG
TGTCTCAGATTTTCTAAGAACCAAAAAAAAAAAAGGAAAAAAAACACAAAACAACAAAAACCAAAAAAAAAAAAAATCACAAAAACAAAA
AAACTATAAAAAAGAAAGAATTAAAAACTTTCAGAGAATTACTATTTACTTTATTAACTTACGGATTTATTATATAAATATATATTCACC
TAGCAACATATCTCTGCCGTCTCTCCTGCTCTCATAATGAAGACATAGCCGATTCTCTGCCCGGGCCCCTTGCTGATGCTCCTCCGGGTC
TGCGTCGGGCGTGGGTCTCTGGGGACCCTCCAGAGGTGGAGGTGGGCTGATGGCCTGGCTGCCTGGTGGTTGATGGTTTTGCTCCCCCTA
CCTTTTTTTTTTGAGTTTATTCTGATTGATTTTTTTTCTTGGTTTCTGGATAAACCACCCTCTGGGGACAGGATAATAAAACATGTAATA
TTTTTAAGAAGGATTCCTGCAGCATCATCTTTTTTTATTTCTCCTGTGTCTGTCCTCCACCTTCTAGGAGAGCCAGGGCAGAGCTAGCAC
TGTCTTAAGCTGTCAACGTGGACTAGCTCGTGTCATCTGCTCGAGAAGGGCTGTCGCTGTTCTTGTTTCTGAGTGAGGAGTACGCAGGCC
AGAGTGGTCACCCGGCCGTGAGCAGTGAGGGCCAGAGACTAGCCCCAGACAGGTGGATGCCAGAGAGAGTGGCACCCATGCCAGGCAAGG
CCTAGGGAGGTGGTCTTGCTCAGCAACCCTGCCCTGAGCAGCAGGTGCGCCCATCCGGAGATCCTAGGAGAAGGTGGCCACCTCCATCCA
CTAAGGAAGAGAAGGAAGACAGTGGCTTGAGGCAGGGAGCTCGCAGGACAGAGCCTGAGCCACCCTGTCCCTCCCACCTGGCTCACCTGT
CTGTGGGTCAGGCAGATGACCCCCTCACCCCCATCCAGGTGCTGGCTGCAGTGGCCTGTGCAGAGAGGGGCAGGGTGAGTGCCCACCAGT
CCCCGTACCCCTTCCCCCCATGCCCCACCATGCCGGGGTGGTACTCACAGAAGATGCAGGTGAGGTGGGCCACACAGGACACGAACCGCT
CGAAGTCCACACGCAGACGGCTATCCCGGTAGCGGCTGGTGAGGGTCTGGGTCAGCTGGTTGTTCAGGTGGAAGCCTAGGGGGAGGCTGC
TTCTGAGCCCAGTGGCCCACAGGGAACCCACCTTGGCATTGCATCCCCACCCCACCTCCTCAGGGAGGACATGAATGCCACAGGGTGTCA
CACCCCAACTCTGCCCCATCCCGGCAGGGTGAGTACAGGCTGATCCCTTCAATCCTCTGGGCCTGTTTCCTCATCCATCAAACGGACTAA
GACAGCCCCGACCCCATAGGGTGTGTGAAGACAGAACGCTCAGGACAGCGTCTGACACGTGACTCGATGTGTGGGTACTGTTACTCCTTA

>63059_63059_3_PC-ACTN4_PC_chr11_66631245_ENST00000393960_ACTN4_chr19_39191240_ENST00000252699_length(amino acids)=1337AA_BP=480
MLGSFFHLPPQIVSAFWRADQTLRMLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAI
YSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEAR
AIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIE
VQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVT
EEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLL
VKVIAHGKDHPTAATKMSRALAEFRVRGVKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNK
ALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHR
HRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHL
MEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDIN
NGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIA
AIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIE
GLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQF
ASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIA
RTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDF

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>63059_63059_4_PC-ACTN4_PC_chr11_66631245_ENST00000393960_ACTN4_chr19_39191240_ENST00000424234_length(transcript)=3054nt_BP=1650nt
GTCAGTGGAGGCAGCAGCGGTAGAGGCGGCGGCGAGGACTGGCGACGGCGAGGAGCTGCCTTCTAGTCTCACCTGTGGACACTTTGCCAA
AGGAGTCGGGCCTGACGGTGGAAGATGGGAAATGGCCAAAAGAATAAACCATTTCCTCTTACTTACCGAATGAAAAGCTTGCTTCATCTT
GGCGTTCAAGACTCTCTAATATGATTCCACCTGTTGGGCTCTTTCTTCCATTTGCCTCCGCAGATAGTGTCTGCCTTCTGGAGAGCTGAC
CAAACACTAAGGATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGGGAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCC
CCAAATGTCCGGCGCCTGGAGTATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGTTCCGGGCCTGC
ACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAGGACACGGGCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATC
GGCCGCGGCCTGGCCCCCGTGCAGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGCACCCT
GGCTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAGGGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTC
CGCAAGATGGGAGACAAGGTGGAGGCCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCCCCATCACGTCC
CTGCATGAGGCCCACGAGTTCTCCAACACCTACGGCTTCCCCATCATCTTCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTG
GTGCACAGCTACGAGGAGCTGGAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGTTTGTGGAG
AAGTTCATCGAGAAGCCACGGCACATCGAGGTGCAGATCTTGGGGGACCAGTATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCC
ATCCAGCGGCGGCACCAGAAGGTGGTCGAGATTGCCCCCGCCGCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCAGCGACTCTGTG
AAACTCGCTAAACAGGTGGGCTACGAGAACGCAGGCACCGTGGAGTTCCTGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAAC
TCCCGCCTGCAGGTGGAGCACACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCACGTGGCTGAGGGCAGGAGC
CTACCCGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAACGGGTGTGCCATCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGC
TTCCAGCCGGACACCGGCCGCATTGAGGTGTTCCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCTTCCAAGGAGCC
GTCATCTCGCCCCACTACGACTCCCTGCTGGTCAAAGTCATTGCCCACGGCAAAGACCACCCCACGGCCGCCACCAAGATGAGCAGGGCC
CTTGCGGAGTTCCGCGTCCGAGGTGTGAAGACCTTCACGGCATGGTGCAACTCCCACCTGCGGAAGGCAGGCACACAGATCGAGAACATT
GATGAGGACTTCCGAGACGGGCTCAAGCTCATGCTGCTCCTGGAGGTTCACGAGCTGGATTACTACGACTCCCACAATGTCAACACCCGG
TGCCAGAAGATCTGTGACCAGTGGGACGCCCTCGGCTCTCTGACACATAGTCGCAGGGAAGCCCTGGAGAAAACAGAGAAGCAGCTGGAG
GCCATCGACCAGCTGCACCTGGAATACGCCAAGCGCGCGGCCCCCTTCAACAACTGGATGGAGAGCGCCATGGAGGACCTCCAGGACATG
TTCATCGTCCATACCATCGAGGAGATTGAGGGCCTGATCTCAGCCCATGACCAGTTCAAGTCCACCCTGCCGGACGCCGATAGGGAGCGC
GAGGCCATCCTGGCCATCCACAAGGAGGCCCAGAGGATCGCTGAGAGCAACCACATCAAGCTGTCGGGCAGCAACCCCTACACCACCGTC
ACCCCGCAAATCATCAACTCCAAGTGGGAGAAGGTGCAGCAGCTGGTGCCAAAACGGGACCATGCCCTCCTGGAGGAGCAGAGCAAGCAG
CAGTCCAACGAGCACCTGCGCCGCCAGTTCGCCAGCCAGGCCAATGTTGTGGGGCCCTGGATCCAGACCAAGATGGAGGAGATCGGGCGC
ATCTCCATTGAGATGAACGGGACCCTGGAGGACCAGCTGAGCCACCTGAAGCAGTATGAACGCAGCATCGTGGACTACAAGCCCAACCTG
GACCTGCTGGAGCAGCAGCACCAGCTCATCCAGGAGGCCCTCATCTTCGACAACAAGCACACCAACTATACCATGGAGCACATCCGCGTG
GGCTGGGAGCAGCTGCTCACCACCATTGCCCGCACCATCAACGAGGTGGAGAACCAGATCCTCACCCGCGACGCCAAGGGCATCAGCCAG
GAGCAGATGCAGGAGTTCCGGGCGTCCTTCAACCACTTCGACAAGGATCATGGCGGGGCGCTGGGGCCCGAGGAGTTCAAGGCCTGCCTC
ATCAGCCTGGGCTACGACGTGGAGAACGACCGGCAGGGTGAGGCCGAGTTCAACCGCATCATGAGCCTGGTCGACCCCAACCATAGCGGC
CTTGTGACCTTCCAAGCCTTCATCGACTTCATGTCGCGGGAGACCACCGACACGGACACGGCTGACCAGGTCATCGCTTCCTTCAAGGTC
TTAGCAGGGGACAAGAACTTCATCACAGCTGAGGAGCTGCGGAGAGAGCTGCCCCCCGACCAGGCCGAGTACTGCATCGCCCGCATGGCG

>63059_63059_4_PC-ACTN4_PC_chr11_66631245_ENST00000393960_ACTN4_chr19_39191240_ENST00000424234_length(amino acids)=947AA_BP=480
MLGSFFHLPPQIVSAFWRADQTLRMLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAI
YSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEAR
AIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIE
VQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVT
EEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLL
VKVIAHGKDHPTAATKMSRALAEFRVRGVKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVHELDYYDSHNVNTRCQKICDQWDA
LGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEA
QRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLE
DQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASF
NHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITA

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Fusion Gene PPI Analysis for PC-ACTN4

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Still interaction with
Tgene	ACTN4	chr11:66631245	chr19:39191240	ENST00000252699		0	21	108_126	54.0	912.0	VCL
Tgene	ACTN4	chr11:66631245	chr19:39191240	ENST00000390009		0	14	108_126	0	693.0	VCL
Tgene	ACTN4	chr11:66631245	chr19:39191240	ENST00000390009		0	14	12_26	0	693.0	VCL
Tgene	ACTN4	chr11:66631245	chr19:39191240	ENST00000390009		0	14	40_61	0	693.0	VCL

- Lost PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	ACTN4	chr11:66631245	chr19:39191240	ENST00000252699		0	21	12_26	54.0	912.0	VCL
Tgene	ACTN4	chr11:66631245	chr19:39191240	ENST00000252699		0	21	40_61	54.0	912.0	VCL

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs for PC-ACTN4

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

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Related Diseases for PC-ACTN4

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source