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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:PKN1-MGAT4C (FusionGDB2 ID:65774)

Fusion Gene Summary for PKN1-MGAT4C

check button Fusion gene summary
Fusion gene informationFusion gene name: PKN1-MGAT4C
Fusion gene ID: 65774
HgeneTgene
Gene symbol

PKN1

MGAT4C

Gene ID

5585

25834

Gene nameprotein kinase N1MGAT4 family member C
SynonymsDBK|PAK-1|PAK1|PKN|PKN-ALPHA|PRK1|PRKCL1GNTIVH|HGNT-IV-H
Cytomap

19p13.12

12q21.31-q21.32

Type of geneprotein-codingprotein-coding
Descriptionserine/threonine-protein kinase N1protease-activated kinase 1protein kinase C-like 1protein kinase C-like PKNprotein kinase C-related kinase 1protein kinase PKN-alphaserine-threonine kinase Nserine/threonine protein kinase Nalpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase CN-acetylglucosaminyltransferase IV homologN-acetylglucosaminyltransferase IVcN-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVcUDP-N-acetylglucosamine: al
Modification date2020032920200313
UniProtAcc.

Q9UBM8

Ensembl transtripts involved in fusion geneENST00000242783, ENST00000342216, 
ENST00000587429, 
ENST00000549405, 
ENST00000602941, ENST00000332156, 
ENST00000393205, ENST00000548651, 
ENST00000552435, ENST00000552808, 
ENST00000604798, 
Fusion gene scores* DoF score10 X 7 X 8=56016 X 13 X 4=832
# samples 1214
** MAII scorelog2(12/560*10)=-2.22239242133645
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(14/832*10)=-2.57115670119613
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: PKN1 [Title/Abstract] AND MGAT4C [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointPKN1(14581494)-MGAT4C(86727905), # samples:1
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgenePKN1

GO:0006357

regulation of transcription by RNA polymerase II

12514133

HgenePKN1

GO:0006468

protein phosphorylation

17332740

HgenePKN1

GO:0035407

histone H3-T11 phosphorylation

18066052


check buttonFusion gene breakpoints across PKN1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across MGAT4C (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4OVTCGA-24-2035PKN1chr19

14581494

+MGAT4Cchr12

86727905

-


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Fusion Gene ORF analysis for PKN1-MGAT4C

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-5UTRENST00000242783ENST00000549405PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-5UTRENST00000242783ENST00000602941PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-5UTRENST00000342216ENST00000549405PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-5UTRENST00000342216ENST00000602941PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000242783ENST00000332156PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000242783ENST00000393205PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000242783ENST00000548651PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000242783ENST00000552435PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000242783ENST00000552808PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000242783ENST00000604798PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000342216ENST00000332156PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000342216ENST00000393205PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000342216ENST00000548651PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000342216ENST00000552435PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000342216ENST00000552808PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
5CDS-intronENST00000342216ENST00000604798PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-5UTRENST00000587429ENST00000549405PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-5UTRENST00000587429ENST00000602941PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-intronENST00000587429ENST00000332156PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-intronENST00000587429ENST00000393205PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-intronENST00000587429ENST00000548651PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-intronENST00000587429ENST00000552435PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-intronENST00000587429ENST00000552808PKN1chr19

14581494

+MGAT4Cchr12

86727905

-
intron-intronENST00000587429ENST00000604798PKN1chr19

14581494

+MGAT4Cchr12

86727905

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for PKN1-MGAT4C


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for PKN1-MGAT4C


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:14581494/:86727905)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.MGAT4C

Q9UBM8

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains (By similarity). Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity). {ECO:0000250, ECO:0000269|PubMed:10962001}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for PKN1-MGAT4C


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for PKN1-MGAT4C


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for PKN1-MGAT4C


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for PKN1-MGAT4C


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource