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 | Fusion Gene Summary |
| Fusion Gene ORF analysis |
| Fusion Genomic Features |
| Fusion Protein Features |
| Fusion Gene Sequence |
| Fusion Gene PPI analysis |
| Related Drugs |
| Related Diseases |
Fusion gene:PM20D2-HIPK2 (FusionGDB2 ID:66598) |
Fusion Gene Summary for PM20D2-HIPK2 |
 Fusion gene summary |
| Fusion gene information | Fusion gene name: PM20D2-HIPK2 | Fusion gene ID: 66598 | Hgene | Tgene | Gene symbol | PM20D2 | HIPK2 | Gene ID | 135293 | 28996 |
| Gene name | peptidase M20 domain containing 2 | homeodomain interacting protein kinase 2 | |
| Synonyms | ACY1L2 | PRO0593 | |
| Cytomap | 6q15 | 7q34 | |
| Type of gene | protein-coding | protein-coding | |
| Description | peptidase M20 domain-containing protein 2I(2)-alanyl-lysine dipeptidaseaminoacylase-1-like protein 2beta-alanyl-lysine dipeptidase | homeodomain-interacting protein kinase 2hHIPk2 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | . | Q9H2X6 | |
| Ensembl transtripts involved in fusion gene | ENST00000275072, | ENST00000342645, ENST00000406875, ENST00000428878, | |
| Fusion gene scores | * DoF score | 3 X 3 X 2=18 | 8 X 8 X 3=192 |
| # samples | 3 | 9 | |
| ** MAII score | log2(3/18*10)=0.736965594166206 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(9/192*10)=-1.09310940439148 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context | PubMed: PM20D2 [Title/Abstract] AND HIPK2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint | PM20D2(89856328)-HIPK2(139313785), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | PM20D2 | GO:0006508 | proteolysis | 24891507 |
| Hgene | PM20D2 | GO:0032268 | regulation of cellular protein metabolic process | 24891507 |
| Tgene | HIPK2 | GO:0006468 | protein phosphorylation | 19448668 |
| Tgene | HIPK2 | GO:0006978 | DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator | 14647468 |
| Tgene | HIPK2 | GO:0045766 | positive regulation of angiogenesis | 19046997 |
| Tgene | HIPK2 | GO:0060395 | SMAD protein signal transduction | 12874272 |
| Fusion gene breakpoints across PM20D2 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across HIPK2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | Non-Cancer | TCGA-IP-7968-11A | PM20D2 | chr6 | 89856328 | + | HIPK2 | chr7 | 139313785 | - |
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Fusion Gene ORF analysis for PM20D2-HIPK2 |
| Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| ORF | Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| In-frame | ENST00000275072 | ENST00000342645 | PM20D2 | chr6 | 89856328 | + | HIPK2 | chr7 | 139313785 | - |
| In-frame | ENST00000275072 | ENST00000406875 | PM20D2 | chr6 | 89856328 | + | HIPK2 | chr7 | 139313785 | - |
| In-frame | ENST00000275072 | ENST00000428878 | PM20D2 | chr6 | 89856328 | + | HIPK2 | chr7 | 139313785 | - |
| ORFfinder result based on the fusion transcript sequence of in-frame fusion genes. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000275072 | PM20D2 | chr6 | 89856328 | + | ENST00000406875 | HIPK2 | chr7 | 139313785 | - | 14167 | 560 | 5 | 2809 | 934 |
| ENST00000275072 | PM20D2 | chr6 | 89856328 | + | ENST00000428878 | HIPK2 | chr7 | 139313785 | - | 3027 | 560 | 5 | 2728 | 907 |
| ENST00000275072 | PM20D2 | chr6 | 89856328 | + | ENST00000342645 | HIPK2 | chr7 | 139313785 | - | 1970 | 560 | 5 | 1969 | 655 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000275072 | ENST00000406875 | PM20D2 | chr6 | 89856328 | + | HIPK2 | chr7 | 139313785 | - | 0.001337938 | 0.9986621 |
| ENST00000275072 | ENST00000428878 | PM20D2 | chr6 | 89856328 | + | HIPK2 | chr7 | 139313785 | - | 0.020491138 | 0.9795089 |
| ENST00000275072 | ENST00000342645 | PM20D2 | chr6 | 89856328 | + | HIPK2 | chr7 | 139313785 | - | 0.06271976 | 0.9372802 |
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Fusion Genomic Features for PM20D2-HIPK2 |
| FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints. |
| Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | 1-p | p (fusion gene breakpoint) |
| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page. |
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| Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page. |
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Fusion Protein Features for PM20D2-HIPK2 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr6:89856328/chr7:139313785) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| . | HIPK2 |
| FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}. | FUNCTION: Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. {ECO:0000269|PubMed:11740489, ECO:0000269|PubMed:11925430, ECO:0000269|PubMed:12851404, ECO:0000269|PubMed:12874272, ECO:0000269|PubMed:14678985, ECO:0000269|PubMed:17018294, ECO:0000269|PubMed:17960875, ECO:0000269|PubMed:18695000, ECO:0000269|PubMed:18809579, ECO:0000269|PubMed:19015637, ECO:0000269|PubMed:19046997, ECO:0000269|PubMed:19448668, ECO:0000269|PubMed:20307497, ECO:0000269|PubMed:20573984, ECO:0000269|PubMed:20637728, ECO:0000269|PubMed:20980392, ECO:0000269|PubMed:21192925, ECO:0000269|PubMed:22825850}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - In-frame and retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 1088_1094 | 449 | 1199.0 | Compositional bias | Note=Poly-Ala | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 1088_1094 | 449 | 1172.0 | Compositional bias | Note=Poly-Ala | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 802_805 | 449 | 1199.0 | Motif | Note=Nuclear localization signal 1 (NLS1) | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 832_835 | 449 | 1199.0 | Motif | Note=Nuclear localization signal 2 (NLS2) | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 802_805 | 449 | 1172.0 | Motif | Note=Nuclear localization signal 1 (NLS1) | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 832_835 | 449 | 1172.0 | Motif | Note=Nuclear localization signal 2 (NLS2) | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 873_980 | 449 | 1199.0 | Region | Required for localization to nuclear speckles | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 984_1198 | 449 | 1199.0 | Region | Note=Autoinhibitory domain (AID) | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 873_980 | 449 | 1172.0 | Region | Required for localization to nuclear speckles | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 984_1198 | 449 | 1172.0 | Region | Note=Autoinhibitory domain (AID) |
| - In-frame and not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 199_527 | 449 | 1199.0 | Domain | Protein kinase | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 199_527 | 449 | 1172.0 | Domain | Protein kinase | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 205_213 | 449 | 1199.0 | Nucleotide binding | ATP | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 205_213 | 449 | 1172.0 | Nucleotide binding | ATP | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 97_230 | 449 | 1199.0 | Region | Transcriptional corepression | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 97_230 | 449 | 1172.0 | Region | Transcriptional corepression |
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Fusion Gene Sequence for PM20D2-HIPK2 |
| For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >66598_66598_1_PM20D2-HIPK2_PM20D2_chr6_89856328_ENST00000275072_HIPK2_chr7_139313785_ENST00000342645_length(transcript)=1970nt_BP=560nt GGGTCCTGGAGGCCTCTGGGCGCGTGCGCGGGCGGTCGCTACCTGCGGCCGAGCCAGGGAGCGAGAGGGCGCAGAGGGCAGCGGGCTTGG GCAGCATGAGGCCCGGAGGGGAGCGGCCCGTGGAAGGGGGCGCGTGCAATGGCCGCTCCGAGCTGGAGCTACTGAAGCTGCGCTCGGCGG AGTGCATCGACGAGGCGGCCGAGCGGCTGGGGGCCCTGAGCCGCGCGATCTGGAGCCAGCCCGAGCTGGCCTACGAGGAGCACCATGCCC ACCGCGTGCTGACGCACTTCTTCGAGCGGGAGCCGCCCGCGGCCTCCTGGGCAGTGCAGCCGCACTACCAGCTGCCCACGGCCTTCCGCG CCGAGTGGGAGCCGCCGGAGGCCCGGGCACCGAGCGCCACGCCACGCCCGCTGCACCTGGGCTTCCTCTGCGAGTACGACGCGCTGCCCG GCATCGGCCACGCCTGCGGCCACAACCTCATCGCTGAGGTCGGGGCGGCGGCCGCGCTGGGCGTGAGGGGGGCCTTAGAGGGCCTCCCCA GGCCGCCTCCGCCCGTGAAGACACCAGATGACCATGAAGCAGAGACAGGGATTAAGTCAAAAGAAGCAAGAAAGTACATTTTCAACTGTT TAGATGATATGGCCCAGGTGAACATGACGACAGATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTCATTGACC TGTTGAAGAAGATGCTGACCATTGATGCTGACAAGAGAATCACTCCAATCGAAACCCTGAACCATCCCTTTGTCACCATGACACACTTAC TCGATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATCTGCAAGCGTCGGGTGAATATGTATGACACGGTGAACC AGAGCAAAACCCCTTTCATCACGCACGTGGCCCCCAGCACGTCCACCAACCTGACCATGACCTTTAACAACCAGCTGACCACTGTCCACA ACCAGGCTCCCTCCTCTACCAGTGCCACTATTTCCTTAGCCAATCCCGAAGTCTCCATACTAAACTACCCATCTACACTCTACCAGCCCT CAGCGGCATCCATGGCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGATTTGTGCCCGGCCTGACCCGTTCCAGC AAGCTCTCATCGTGTGTCCCCCCGGCTTCCAAGGCTTGCAGGCCTCTCCCTCTAAGCACGCTGGCTACTCGGTGCGAATGGAAAATGCAG TTCCCATCGTCACTCAAGCCCCAGGAGCTCAGCCTCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTGGGACCCAGC AGATCCTGCTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCCACCCACACATCAGTGCAGCATGCCACCGTGATTCCCGAGACCATGG CAGGCACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGCCATTATAATCCCATCATGCAGCAGCCTGCACTATTGACCG GTCATGTGACCCTTCCAGCAGCACAGCCCTTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCAGCCAACCAGCACCACCTCCTCCCGGA AGAGTAAGCAGCACCAGTCATCTCAAAGAAAAAACGTCATCAGCTGTGTCACAGTCCACGACTCCCCCTACTCCGACTCCTCCAGCAACA CCAGCCCCTACTCCGTGCAGCAGCGTGCTGGGCACAACAATGCCAATGCCTTTGACACCAAGGGGAGCCTGGAGAATCACTGCACGGGGA ACCCCCGAACCATCATCGTGCCACCCCTGAAAACCCAGGCCAGCGAAGTATTGGTGGAGTGTGATAGCCTGGTGCCAGGTAATTTGGGGC >66598_66598_1_PM20D2-HIPK2_PM20D2_chr6_89856328_ENST00000275072_HIPK2_chr7_139313785_ENST00000342645_length(amino acids)=655AA_BP=172 MEASGRVRGRSLPAAEPGSERAQRAAGLGSMRPGGERPVEGGACNGRSELELLKLRSAECIDEAAERLGALSRAIWSQPELAYEEHHAHR VLTHFFEREPPAASWAVQPHYQLPTAFRAEWEPPEARAPSATPRPLHLGFLCEYDALPGIGHACGHNLIAEVGAAAALGVRGALEGLPRP PPPVKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLD FPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQAPSSTSATISLANPEVSILNYPSTLYQPSA ASMAAVAQRSMPLQTGTAQICARPDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGLLAQQAWPSGTQQI LLPPAWQQLTGVATHTSVQHATVIPETMAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKS KQHQSSQRKNVISCVTVHDSPYSDSSSNTSPYSVQQRAGHNNANAFDTKGSLENHCTGNPRTIIVPPLKTQASEVLVECDSLVPGNLGPG -------------------------------------------------------------- >66598_66598_2_PM20D2-HIPK2_PM20D2_chr6_89856328_ENST00000275072_HIPK2_chr7_139313785_ENST00000406875_length(transcript)=14167nt_BP=560nt GGGTCCTGGAGGCCTCTGGGCGCGTGCGCGGGCGGTCGCTACCTGCGGCCGAGCCAGGGAGCGAGAGGGCGCAGAGGGCAGCGGGCTTGG GCAGCATGAGGCCCGGAGGGGAGCGGCCCGTGGAAGGGGGCGCGTGCAATGGCCGCTCCGAGCTGGAGCTACTGAAGCTGCGCTCGGCGG AGTGCATCGACGAGGCGGCCGAGCGGCTGGGGGCCCTGAGCCGCGCGATCTGGAGCCAGCCCGAGCTGGCCTACGAGGAGCACCATGCCC ACCGCGTGCTGACGCACTTCTTCGAGCGGGAGCCGCCCGCGGCCTCCTGGGCAGTGCAGCCGCACTACCAGCTGCCCACGGCCTTCCGCG CCGAGTGGGAGCCGCCGGAGGCCCGGGCACCGAGCGCCACGCCACGCCCGCTGCACCTGGGCTTCCTCTGCGAGTACGACGCGCTGCCCG GCATCGGCCACGCCTGCGGCCACAACCTCATCGCTGAGGTCGGGGCGGCGGCCGCGCTGGGCGTGAGGGGGGCCTTAGAGGGCCTCCCCA GGCCGCCTCCGCCCGTGAAGACACCAGATGACCATGAAGCAGAGACAGGGATTAAGTCAAAAGAAGCAAGAAAGTACATTTTCAACTGTT TAGATGATATGGCCCAGGTGAACATGACGACAGATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTCATTGACC TGTTGAAGAAGATGCTGACCATTGATGCTGACAAGAGAATCACTCCAATCGAAACCCTGAACCATCCCTTTGTCACCATGACACACTTAC TCGATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATCTGCAAGCGTCGGGTGAATATGTATGACACGGTGAACC AGAGCAAAACCCCTTTCATCACGCACGTGGCCCCCAGCACGTCCACCAACCTGACCATGACCTTTAACAACCAGCTGACCACTGTCCACA ACCAGGCTCCCTCCTCTACCAGTGCCACTATTTCCTTAGCCAATCCCGAAGTCTCCATACTAAACTACCCATCTACACTCTACCAGCCCT CAGCGGCATCCATGGCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGATTTGTGCCCGGCCTGACCCGTTCCAGC AAGCTCTCATCGTGTGTCCCCCCGGCTTCCAAGGCTTGCAGGCCTCTCCCTCTAAGCACGCTGGCTACTCGGTGCGAATGGAAAATGCAG TTCCCATCGTCACTCAAGCCCCAGGAGCTCAGCCTCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTGGGACCCAGC AGATCCTGCTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCCACCCACACATCAGTGCAGCATGCCACCGTGATTCCCGAGACCATGG CAGGCACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGCCATTATAATCCCATCATGCAGCAGCCTGCACTATTGACCG GTCATGTGACCCTTCCAGCAGCACAGCCCTTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCAGCCAACCAGCACCACCTCCTCCCGGA AGAGTAAGCAGCACCAGTCATCTGTGAGAAATGTCTCCACCTGTGAGGTGTCCTCCTCTCAGGCCATCAGCTCCCCACAGCGATCCAAGC GTGTCAAGGAGAACACACCTCCCCGCTGTGCCATGGTGCACAGTAGCCCGGCCTGCAGCACCTCGGTCACCTGTGGGTGGGGCGACGTGG CCTCCAGCACCACCCGGGAACGGCAGCGGCAGACAATTGTCATTCCCGACACTCCCAGCCCCACGGTCAGCGTCATCACCATCAGCAGTG ACACGGACGAGGAGGAGGAACAGAAACACGCCCCCACCAGCACTGTCTCCAAGCAAAGAAAAAACGTCATCAGCTGTGTCACAGTCCACG ACTCCCCCTACTCCGACTCCTCCAGCAACACCAGCCCCTACTCCGTGCAGCAGCGTGCTGGGCACAACAATGCCAATGCCTTTGACACCA AGGGGAGCCTGGAGAATCACTGCACGGGGAACCCCCGAACCATCATCGTGCCACCCCTGAAAACCCAGGCCAGCGAAGTATTGGTGGAGT GTGATAGCCTGGTGCCAGTCAACACCAGTCACCACTCGTCCTCCTACAAGTCCAAGTCCTCCAGCAACGTGACCTCCACCAGCGGTCACT CTTCAGGGAGCTCATCTGGAGCCATCACCTACCGGCAGCAGCGGCCGGGCCCCCACTTCCAGCAGCAGCAGCCACTCAATCTCAGCCAGG CTCAGCAGCACATCACCACGGACCGCACTGGGAGCCACCGAAGGCAGCAGGCCTACATCACTCCCACCATGGCCCAGGCTCCGTACTCCT TCCCGCACAACAGCCCCAGCCACGGCACTGTGCACCCGCATCTGGCTGCAGCCGCTGCCGCTGCCCACCTCCCCACCCAGCCCCACCTCT ACACCTACACTGCGCCGGCGGCCCTGGGCTCCACCGGCACCGTGGCCCACCTGGTGGCCTCGCAAGGCTCTGCGCGCCACACCGTGCAGC ACACTGCCTACCCAGCCAGCATCGTCCACCAGGTCCCCGTGAGCATGGGCCCCCGGGTCCTGCCCTCGCCCACCATCCACCCGAGTCAGT ATCCAGCCCAATTTGCCCACCAGACCTACATCAGCGCCTCGCCAGCCTCCACCGTCTACACTGGATACCCACTGAGCCCCGCCAAGGTCA ACCAGTACCCTTACATATAAACACTGGAGGGGAGGGAGGGAGGGAGGGAGGGAGAGAATGGCCCGAGGGAGGAGGGAGAGAAGGAGGGAG GCGCTCCTGGGACCGTGGGCGCTGGCCTTTTATACTGAAGATGCCGCACACAAACAATGCAAACGGGGCAGGGGCGGGGGGGGGGGGGGG GGCAGAGGGCAGGGGGACGGGTCGGGACACCAGTGAAACTTGAACCGGGAAGTGGGAGGACGTAGAGCAGAGAAGAGAACATTTTTAAAA GGAAGGGATTAAAGAGGGTGGGAAATCTATGGTTTTTATTTTAAAAAAGAAAAAGGAAAAAAAAAAAGTCAATAACAAAAAACCCAGCTC AAGAACCCATTCTACGCCAAACTGGAAAGGAGAAGAGAGCAACAGGAAGATTCCAGAAACGGGGGGCCCCAGTTTTTGAAGAACTTTATG AACTTTTCAAAGATTATTTTCATATGGCAGCAAGTGATACGGAAGACTGCTGTCAGGGACACCTGATATGGAAATCAAATAGATTTTTAA TTAATTGAACATAAGATTTAGGGATTTTTCCAGAACTCGAAAGGGTCAACAGCCCTCCAGAATGTCGGGCTGCAGCCTGAGGAGGCTGAT GTTTGGAGCTGGTGTGGGATTGGCGAAGCCCAGTCCGGGCTCCCTAGTCAGGAAAGACGGGGGACGGCCAGGCTGCTGGAAGGCCCCCGG GGGCGCGGGGCGAGTTTTCTTTTTCTGAGCACTCTGGATAAATCCCTAAGCAACGTTGTTTCTCAAATGTCATTAATAATGTGTGTTGCA AACTTTAGGTTTTTTTCTTTTCTGAAAATGTATTTTCTCTTTGAATCCACCCCTAGTCGCGTAGCGTAGGGCTAGCGGTCGTCACAGACA CCCTAGTAGAATGTAGCACTCAGCACCCTTGTCTCCTACCTTGTGTTCAACTCCAATGATACCAATAGAATATTCCTCAATGTAATTGCA CAAAAAAAAGCGATATAACATAGGCATGTAACCAATGTGGCGGTGCAGGTGTGCGGGTGAGCGAGCACGTGTGGGTGTGCACGCGCCGCC CTCCCCGCGTGGCCCTCGGCGCCGCCACCCTAGCTGGCGCAGTCTTGACACTGCATCCTTCCCTCCTAGTGCCTTACCGAGCGACAGACG CGGCGTGAGGGTTTACTTCCACTGGTACTCCAAGAAACTGAGGCTAGTCAGACACAATCTCAGCTCTTCTGTTGTGCTGTTGTAACAGTT TACGCTGGCCTTTTTTTTTTCTTTTTCTTTTTCTTTTTAAAATGTTAATGCCCGTTGTCTTTCCTGGGCTGTTTGCTAGCGGAAGGATGC CAGGGAAGCCAGCAGGAGCTAGGAGAGAGTCCGTGGATCTCGAAAGAAATATGGGAGACAGATGCCCGGCGGGTGCGTCTGGAGATGGGG ACGGCGGGAGTTGAGTTGTGGCAGTAGTTGAGTTGTAATTTGTGGGCGGAGGCCCAGAGAGACTCCCCACCCTTCACCCCTGCCCCACTC TGTCCCCAGTTCCGCCATTTGTGAGGCCAGAGGTTTCCGGACTGTTGGCCTCGCCAGGCAGCCGTCTCCCGCCCCAGGCGGCATCCCCCA GTCCCTCCCGCCTCCACGAGAGCCTGGAGCTCTCAGCCTCGCCCGGGGCTCCACTCTCTCCTCCGGCTCCCTGGGCTGTTTTGCTCTAAC GATCTTGCCAGATCCCTCCCTCTGTAGACAACCACCAACCTCTGTTTGCTGTTGAATTCTCTCCTCACATTACCCAGGTCTGCTCAAGAC ATGATTTTGGTTTTGGTTTCTGAGGGTTCTAGTGGGCAGAAGGTTGGAGGGACACTTATGAGGGTGGCCGGGGGTCTGACGCTGCACTTT GGAAAAACTCACACAGTTGAATTTCCAAAGAAATCTGCCCTTTGCCCTCTTTGCACCTTTGATACATTCTGGAAGTTTTCTCAGGCTTTG GACACTTCTGGGGATGGAGGTGTGGAGAAGTGGGGAGTTCCCTCTCTTCATAGTAAATAACTCTGAAATATGTGAATGTGAATGGCAGGA GAATCTGGCCAAGGATGGGGCCGAAAAGGGTGGTTCTAATTGTTTGCTTCTGATGTTGAGTCTTTAGCTGACCCCACAGGCAGGTTTCCA AGGTGCAAAGAGATCTTTCCCGAGTCAGCGGCCCCATCCTCATCCTCCCTCCCTTTACTTCCTCACTGTGCAGTCTCCCTCAAGGATCTA CTGTGAAAGGTGTGTTTGTAGTGATATCCAACCTAACTCAGTAACGAAGTCGTTACTTAGCTCTTAGCTGTGAAATAACTCTGGAAACTT CCCCACCCCAACCATAAATTCTTACTTATAAAGAAACAGGTCCCCAAACTGGAAACAGCTTAGTCCAGGCCTCAGCGAGAAGGAAGGACA CCATGACTGCTCCATGCTGGGCACAGCCGGGCAGTCTTGCCAAGTGCCTGCTGGAGGCTGTGCCGGCAAGAGGCCTGCAGCAAGGAGATT CCCTTCCCTCGGGCCATTATCAATACTGTCTTTATCTGGAGGTGGGGAAGCGCAGCCCTCTGAGACAGCAGGACAATGGTCAGTTCAGAG AGGGTGAGGGCAGCAAACGCTTCAGAGGACACAGAAGCCAGAGGACCCCCCCCCGCCCCACAGCTGGGTCAGCCTGGAAAATCCATCTAT TAGGGACTTTTTGGCAGCCAGATGGCAGCAATAGCCCATTAGGTCTCATCCCGAGTTCCAAGTCTTGGCTGCAAATGAGCCTCAGTTCGC CTTACTGGAGAGCACCCCCAGATTCCTGGGCACAGTTCATTTCCAGCCCTTTCTAGATCTGATCTTTTAGGGGGAAAGACAGCTTAAAAT GTTCTTTTCATTTTAAAGAAAATTATTCTGTCTGCTTAAGTTGGAGGCTACTTACTCTTTCACCTGACATTTTCTTTCCTTTTATTCTTC CAGATCAGGAATGAAATTTCCATGCTGCTCATAAAGATAATATTATTGTACTAATTATTTTTATTACCATTGTAATTATGATCATTATGT TGATATTTTAGTCAGGGTTTTAAATGCACATTTATTCCAAGTATCTTTGTGTTTTCTCTTTAATATTTAAACTTATTCTCTCTGTGAGTA TATAAGTAGACTGGAGGGACATCCAGATGTCCAGTTTTGTCAGGCAAAAAAAAAAAGGAAAGACTTAGGAAGTAGGAAAATTGTTTCTGT CATCTCTATCCCAACAAGAGACGTCAAGAAAGATCCACCACAGAACAAAAGTTTAAAGAAGAATCAAAGCCTTGATTGGGCTTCTGACAA CATGGTCACCATCAAGGTTGTCATTTTCTAGATCCCAGAGGCCTGGGATGCGACGTCAGGTGGCATCTCATGGGCTCGGGGAATGTCGAG TCACTGACTGTCCAGCCCTTAGCCAGCTTCTCTCCCACATCCTCAGAGCTCTCCTGTGCTTCTGAAATCTGTTAACTAAATCTTTGGCTT GCCTCTGGTATTTAAGCAAGAAAATTCCCTCCCAGAGGTGACCCCATCCGCTTCCCCACAATCCATCCTTTTGCCATCGGCGCACCTGGG GCGTGGCTTAGGTTCTTCAATGCAGGGACATTTGCCCCCTCCCAGAAGCTGCTGGGCACAGTGAGGTGGCGTAAGAGTGACTGGCAGGTG GTACCTTCCCCAGGAAATTTCACCACACCACCCAGTTCCTCAGCCTGCCCCCTCCCCCTGTGATGCATGCCCCCAGCACCCAATTCTAGC CAGCTGGAAGTGGGTGGAGGGACAGCAGGAGGCCAGAGAAACCCTGAACAAAGCTGGGCGGCTGCTCAGGCATCACAGGCTGCACCCCCT CTGAAAGCATCCCCACTGGGCTCCGGCCACATCTTCAGTGCACTGTGCTGTGTGCGCTGGGTGCTCACACGCTGTCCCCAGACCCACAAA GTGCTAGGCCCCAGTTGAAGAAAGGGGTGAAATAGCCAGCTTCACCGAAGGGAAGGGAAGGGAAGTATTGGGCGATGCCAGCCCCACAGA CGCTCAGCAAACATTAGTGCACATTCTCCTAGTCCTCACCCAATGGCCTCCTCTACCCCCATGCATGGAGCTGCCACATCAGAAGCCCCA AGAGAAGCTCCCTGCAGGAGAGGCCAGCTCCCTGGATGCCCAATTGCATACCTGGCCGAATCTGCCATTGAGTCACCTTAGCAAATAGGC TGCTGTCACTAGGACCAAGCTCTCAAGCAGAGGGATGCCAACCTAGTCCTTACTTAGCCCACGAATCATCTAGAGCATCCTCTAGTCTTT TGTGGGCTCCCTCCTTCCCATTTGAAGAGACATTGTTCAGAGGAAGAGGGGAAGATTTGAAATGTCAGGTCACGGAGGAGTGTTTAACTG GAGCCTGGTGAACCGCAGGGCAATTTGCTTCTGCTCACTGGGTTCTGACTGGCCCGTCTGGACGTGGGCCCCCATGTCTCTGTGCTTAGG GCCTCTTCATGATGTTTTGGATGTTTCCAAGGGAAGTGGGTGAGCAGATCAAGGGGTGGGAGAGTCGAGGCTTGATGCCAGTTAATACTG TGAAGTGGAGCGTGCGGTCAGTGGAATTCAGAGGAAAAAGAAGGGTTGGAGCAAAGCGGCATTCATCTCCTGGACTGTTAGCCTTTCTAG TCTTCCTGGTGGCTGAGGTGTTCACGGGCTGGGGGAGCCAGCTGACCTTTGTCCTCTTCAACCTAGAAGACTCAGCCCGCCCAGACACCA ACGTGTGAGACGGATGGACATCAGGAAGGGAAGGGGAGATTAGCCCAACTGCTGACAGAACGATTTCCCTTGGTTGGACCTTGGGAATGG CAAACACTCATATTGGAACAAGCTTGGGGTGGAAGATTTAGGCCGTGTGAGCATGTGTGAGTGAGTGGAACAAACTTTCTTGGAAACTGG AGGGAGGAGATGAGGAGGCTTCGGGAAGTATTACTGATGGCTCATGGTTGAGAGAGCGACGTGGGGACCCAGCTCGCCCCAGCTTTTGTC CCAGGTTCTCTTTGTCTGATGCTGAGGGCAGGGTGGGGTGTGGGACCACCACTCTTGTTGGCCTGTCAAGTAGACCCTAGGACAGAAAAT GGAAAGAAGGAAATGGCTCGGTGCTCTCAACTAGCAGAGAGAATTGAGGAGAGGTAAGGGTTCCTTCTGCAGGCCAGCCTGGGACTCCAC AGCGCCAGCAGGAGTGACTTGGCCACAAGACATTCCAGCCCCAGGGACTTTGCAGGCTTCATTCCCTGTCTGTGTCTTTTCCTTCTGGTG TGTTTTACAGACTTCTGATGGGGAAGCTTCAAACTTGAGCAGGCCAGAGATGTCCTTACCAAATTGGAAAGGAAGGTGAAACTGTTCCTT TCTTTAGCCAAAGAACCCTTCTCAAAGACGCCTCCAGAAATGGACAAAATGGCCTTCCCTTCGTTCCTTTCCAGGCAATAATGACATCAT TAGTGATGCAATTCTATTTGTCTTTCTCTTTCCTCTCTGTCCTTTTTTTTAAAAAAAAAAAATGCATTTATTTCAAAACTGTGCTATTCT TTTAAGAGGAGTGGAGGTGACCCCTTCGATGCTGCTGCTATCGGGAGACAAGGTGCCATACCAATACGTGGGCTTGACTAATCCCAGGCC ACCATGGGAGAGAGCAAAGCAGGGCTGCCAGGAGTTCAGTTGCATCAAGGGCGTAGAGCACGCGGGGGCTGGGCTCGGAATAGCAGTACT TTTCCACTTTGATGCCTTAGAACTCTCACTTCTCATCTCCACAGACCAGACTCAGTAAAATCTCAGGCCACTAGAGAATGGAAGGCGGTG AAACAGGATTTAAATGCAAAAAAAACCTATTGGAGGCTTTTGGCACCGTGGCTCACTAGAGGGACCCAGCATAGTAGAGGTTTCTCTTGT TGCAGCTTCTGAAAAGTTCAAAAAAGAACTCCAGGCCGTTCTTCCCTCAAACCCAGTGAGAGTTTGCAGAGAAGTGCCCCCTGCAGGGCT CCCGTCCCAGAACACCAGCACCAGAGAGGGTCTTCCCGATGCCCCCCGCTGGACTTGCCCAAGCCTCTGGGAGCCCCTCATCTCAGATCC CTGTGTTGAACATGACACTGACTGTCCCTTATTTGTTAAAATTTGCAATATCTCTCAAGTAAATAATAGCCAACATTTGTTGAATGCTTT CATGACTCCCGGGCTAAGGCCTTTATGAGCGTTGTCTCAAGGGGCCCCAACAGCCATCCCACAGGGAGGGGGATAACAGCCCCCATTTAT AGATAAGGGAGCTGACCGGATGCTCTGAGAAGTGGCAGGTGTTGAAGGAAGGATAAAGCAGTGATGGGCCAGAATCCCCAAGGTTCCCTT TTTTGTTCATCAGGCCCTTCCTGAGATGTGATTTTTAATCTTTTAACTTTTTTTAATTAATAGCAATGCGTGGCCTCATATTTCTATGAA CCATTTAGTGATACTCCCGCTTCCTGCATGCCACACACTGTGCTGGGAATTGCTCATGGGTTGTCCTGTTTCATCTTCTCCGTAGCCCTG TGAGATCGGCAATATTAGTCCCCCTACAGCCAAGGAAACTGCCCAGAGCCACACAACTCTTGAGGGGCGAGGAGGGCTTGAACCTGAGTC TGCCCAGCTCCAGAACTGAGCTTGCAGCCATTAGCCACAGCTGTCTCCTGCATGTCTGAGCAAAGAAAGGCCTTTACACAGCATCACCCT GTGCCATCCCATGCACCGTGGGACTCAGCTAAAGGACTGTGCAAAGAGGGGGCTCCTGAGTTGGATTTAGGCAAAAGGGGCAGAATTCGT TTGATTTTTAGAGAAAATCTCTGGAGAGTTTCTTTTGATTCATAGAATTCCTTTTAGATTTCTTTCCAGCATACCAACTAGCTTTAGTAG TGCTGCTACAACCAGCTCTTATAAGTAAGAGTGAAAAAGTATTCTTTTCTTCTTTAAAAAATAAGTTTTTCTTGCTTATAGTTAATTCTA GAAAGGCAATACTAAAGGTATATATTTTTTTCAAAATGCTATTTTTTACTGCACTTGATAATTATCCTGACAGCTCTGATCTCTGTAATA GATTCACTCTTCAGCTCTGGGCAGAACCAGAGGCAGGGTTCACACCAAATTTGTAAATACCATATGTGGGTCTGGTGTCCAGGAACTTTT TTCTTTCTGTTAAAAAAAAGAAAAAAAAAAGAAAAAAAAAAAGAAGTAGAGGTGGAAGAAAGACAAGACTTAGAGGAACAAAAGAATGTT TTCTTTTGAGATACTCTTCTCAAAGAAATAGCAACAATTGTATAAACAGGAAAACCAGCCAGCTTTCATGATAAAAGGAAGGCGTGTCTC TTGCCCTGGTATGAGATTAACAGAAATACAGATGCATTTTTATTTTGATTGAAAGATGGTGAGAATGTAGAAATGCTTAGGACTAGATTT TTAATTTTTTAAAATAACTATTATCATTTATTATGAAATATTTGTTCAGTTGTTTTGAGTGGGTTTCTTGTTCCTTTTTTCATTTAAAAC CTTCTTTGTTGACTGGCTCCAGGCTTGTTTGCCTAAATTCTTAGGTAGTTTACACAAGTTCTAGAATCTTTTAGAACTTTAACTCCATTG GAAGCAAACCTAACTAATCGGAGTTTGAGATCCTGGTTGGTTTCAATAGGTATTCTGGAATTCTGGCAGAACACCTAAAGATTTTTTTTT TTTTTAGAAGGTTTTAGATACATTATCTTACACAAACTGTGACCTAATGGCAATAATTACCTCAAATGTGGGCATTCATCCTGGTTTTAG CCTTTTTTGAAATCATGTAGCCAGCTTGATCTTGGAATTTAAAGACTATGAATTCTCTGTGGGCTGAAAATAATGATTACTTCATACCCC CGGTCATCGTTGCTTAAGTGAATTCTGAAAATAGCTCATCTTTACAACAAAAATTAAACCAAGGAAGAGATTATTCTTTGTGTGTTGTAC TCAAATGCGATGTTCAAATGCACATGTTAAGTATATATGTTTTTAGCTACTGTAAAATGCTGTTAGCCTTCTAAGCTATCAAAACAGTCA CATTTTAAATGAGTAAACTAAACAATTGACTGTGGATACTTAAGCATATTTCTGGCTACGTTTTATAGTTAAAGTGTTTTATAGTTTACA TTTAGACTGGTACTTTTTAAAGAAAAGTTCTGTTTATAACTGACATCCGCAAACCCCAGTGAATGCCTCTTAGTTGGAGGTTGTGTCTCC CCCAAGGCAAGTGTGTTGTCCCAGACTCTTCTGTAGTCCAGCATGCGCACTTCCCTCTGGATTATTACTTTCCACGTGAACTCAAGAGAA CATGAAAGGCAATCCAGATGGAGGGAAAAGGTGTGAGTCCGCAGCCCGGGCCAGATGCGAAGGTCTCATGCGTGTCGTCTAAACACTTGT CTTCAAGGCCTTCTCTCTGACATCTTGGAAGAGTCATTGAGAACAGATAACCTGGTTCATTGATTTTTGTCTTGATTTGAATATTTAACT TATTAATAGATCCACTGATTTCCAGGCACCAGGCAGTAGAAGAGACTGGGATTCAGGTGACCATGAAGGCACAGCTGCTACTTCTGGGCC GGGGGTGATATTTTGATCAGCGTTTTGTAGGGGAGGACCATATACCCCTATTCCCATGGTCGCTGGCTGGGTTTTCCATATATCTGCTGT CATTTATTCGTTTTCCCCTTAAAAGCAAAATCAATGTAAAAGGCTATGTTTACGTTTTACTCATTGTCCAGCTTAGACTCAAAGTCTAGT TCGGTGGGAGGGGGACCTTAGCATCCTCTCAGAGATGGTCAGGGCTGAGCAGGAGGAGGCAGAGACAGAGGGGCAGCTCAGCCTGGTCCA TTGAGACCCACTCTAAACAGACATCATATTTGGAACAAGAAGATGCTTCGAGACAGGAATGGGCCCCACTGTCATGCAGAAACAGACTGG GGGAATGGCAGTTTCCCTGAGTCTTGGTTTCTTTTATGTTTTCTCTTGTGCCACCACCAAACTGCAGAGGACCTGCTGTGACCTAAAGGG CATTCCTTTAGCAGATAAGACCTTGAAAACTGCAAAACACCTGGGACCAGGGAGCTTTTAAAAAATACAAAAAAATACCACATTTGCTTT TTCCCTGTGAACTGTATTGACAGCGTGTTCTTAGGACAGTCTTTTGGTGGAAATGTTACTGTAAAATAGTTTTCATCTCACCCCTCCTAA TCATACTCCCACTTTCCTGTTTGTGTGGTGGTGTTGCTGTTTTTTCCTTTACATGAATTAACCAAATGAATTTTGTGTCATTGTTTTTGG GGCTTATATTTTTAAAACATAGAAATTGCCTTTTGTTCATTTGAAAAGTAAGTATGTTGTATCTGAAAAAGGGCTCTGCCTCTGCTCTCC CTCGCTTCCTTGTAACCAATCTCCAAACGAATCTCTCCTGGCACCGCCCCCTTCCTTATATAGGGTCACTGTCCCCGGGGCCACCTCTGC CTCCACCCTGCTGTCACCACTGCCCTGGGCCAAGGCACCCAGGACTCCCAGAAAGCGCGAGAGCCAGCAAGAAGGCCCCACTCAGCCTTG AGACTGGTGGTCACACCTCCCTGTCAGAGTCGCCTGCTGGGCTGAAGGGGCAATGGATTGTCATTGTTGAAATTGTTTGGCTCAGGTTAT AAGGAGGAACTTGGGAAGTAGAAAGTGACTTGACCATGTGCATCCTTGGTAGCTTCCTGTAACTAACAAATGGAACAGAGAGCACACCCC CGCCCCGCCCCACCCCAAGCAGATGTTCCCGTCAGCGCTGCCCTGAGTCAGTCGGTCCCCCGTTTCTGCTCTCCTCCCTTTTGTGTTCCT GCTCACTTCAAGCTTCTTCCATGGACTTTCCAGGGCACAGTCATCTCTAGCCCCCAAATCATCTCTTCATCCTCTGTGTGTGCATTTTTT TAACCAAATGAAATAGACAAGAAAGTCATACTTTGGGGCAGCAGAATTTCTAATTTAGTAGAATCACTGTATAGAGATAGATGTTGATAT ATATGTTTGTGTATATATATCAAACCAAATTGGATAGGAGAAGTATAGCTTTACAGATGAGGAGAAGGAGCTCTTTAGAGGTCGGAGTCA AGACTGGTGTCTTGGACGTGCATGGGCTGTGTCCCAGGCCACTCCGCACACATGGGGCTGAGGCGTGGCGCCGGGCCCTTGTCATCCACC TCACCACGGCAGAGCCAGCAGGCCCTGTAGGGTGCTGCTGCTGTCTCACTGGGTCCCAGCTTCAAGCGCATCAGTGGGTGACGGGGGCAA CAAATCAGAGTGACTGGAAGTTTCCATCCCGTTTTGCTTTGACCACGTGTACTGAGCTGCAGCCTCTGATACTCTGTCACGTTTCCAAAA ATGGTATCCATTAGGATAGAAAGAGAATGGATCTGCAGAAATGTTTACCTTTCAACTGCTCATGAATTCAGGACACTGGATAGAAAGACT CACTCCCCAAAATGAGAACAGGGAAGAGGAGACCCGGCGACACTAAGTCACCAGGTCCAAGGAACGTGGCTCCCTCCCCAGGGTCATCTC ACCTAGATCTTTCTCTCCCAGGTCATCTCAGCTCAATCTCAATAACCCTATGAAAGCCCTGGTCTGTTGTGTTCCTTCACCGTACGGTTT CTGTAATAAAAAGTGTTAATCCATGTTAATCTGTGTGAAAATTATTGCGTGCAACAGTATTTTCTCGTGTACCTCTTTTTCCTATGTGAA TTGTCCCTCTTTTTTATTTATAAATGTCTACTTTTGTTTTTTTAAAGACAAACCAATGTGTTGTAGACCTATATGTAACCTATTCCTTAG TCTCATATTATAGGTATGTTATAAGAATGGATATTTTACTTGGCTTTAGAATGTTTTACAAGAAAACTAATTCTTAACTGATCAAGTCCT TGCTACTAAAATGCTTGTGTTTTTCATCATGACGTCGTGTGCTTCTAAATTAATCATTTTCGTTGTAGAAAAATGGAGTGAATTTATATT AGTCTTGGAAACTAATAATAGCATTGTAAATTTATGAGATGATTTTAACAGAAAAAATATAGAAGAATATAGTTATTTTAATTGTAATAT TACTAACTGTAGGGTGAGAAAAAGGGGGGGGGGTCCCATTGTGGTGAACTATGTTATAGCTTGTTACTCATAGTTTCTTTTTGATCATTT TTTCGGTCTCCGAGGTGAAATGACTTATTAATTAAAATTTGTAAACTCACATATGCATATTGTATATGTGTAGAAATGTAATCACACTTT >66598_66598_2_PM20D2-HIPK2_PM20D2_chr6_89856328_ENST00000275072_HIPK2_chr7_139313785_ENST00000406875_length(amino acids)=934AA_BP=172 MEASGRVRGRSLPAAEPGSERAQRAAGLGSMRPGGERPVEGGACNGRSELELLKLRSAECIDEAAERLGALSRAIWSQPELAYEEHHAHR VLTHFFEREPPAASWAVQPHYQLPTAFRAEWEPPEARAPSATPRPLHLGFLCEYDALPGIGHACGHNLIAEVGAAAALGVRGALEGLPRP PPPVKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLD FPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQAPSSTSATISLANPEVSILNYPSTLYQPSA ASMAAVAQRSMPLQTGTAQICARPDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGLLAQQAWPSGTQQI LLPPAWQQLTGVATHTSVQHATVIPETMAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKS KQHQSSVRNVSTCEVSSSQAISSPQRSKRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTTRERQRQTIVIPDTPSPTVSVITISSDT DEEEEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYSVQQRAGHNNANAFDTKGSLENHCTGNPRTIIVPPLKTQASEVLVECD SLVPVNTSHHSSSYKSKSSSNVTSTSGHSSGSSSGAITYRQQRPGPHFQQQQPLNLSQAQQHITTDRTGSHRRQQAYITPTMAQAPYSFP HNSPSHGTVHPHLAAAAAAAHLPTQPHLYTYTAPAALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQVPVSMGPRVLPSPTIHPSQYP -------------------------------------------------------------- >66598_66598_3_PM20D2-HIPK2_PM20D2_chr6_89856328_ENST00000275072_HIPK2_chr7_139313785_ENST00000428878_length(transcript)=3027nt_BP=560nt GGGTCCTGGAGGCCTCTGGGCGCGTGCGCGGGCGGTCGCTACCTGCGGCCGAGCCAGGGAGCGAGAGGGCGCAGAGGGCAGCGGGCTTGG GCAGCATGAGGCCCGGAGGGGAGCGGCCCGTGGAAGGGGGCGCGTGCAATGGCCGCTCCGAGCTGGAGCTACTGAAGCTGCGCTCGGCGG AGTGCATCGACGAGGCGGCCGAGCGGCTGGGGGCCCTGAGCCGCGCGATCTGGAGCCAGCCCGAGCTGGCCTACGAGGAGCACCATGCCC ACCGCGTGCTGACGCACTTCTTCGAGCGGGAGCCGCCCGCGGCCTCCTGGGCAGTGCAGCCGCACTACCAGCTGCCCACGGCCTTCCGCG CCGAGTGGGAGCCGCCGGAGGCCCGGGCACCGAGCGCCACGCCACGCCCGCTGCACCTGGGCTTCCTCTGCGAGTACGACGCGCTGCCCG GCATCGGCCACGCCTGCGGCCACAACCTCATCGCTGAGGTCGGGGCGGCGGCCGCGCTGGGCGTGAGGGGGGCCTTAGAGGGCCTCCCCA GGCCGCCTCCGCCCGTGAAGACACCAGATGACCATGAAGCAGAGACAGGGATTAAGTCAAAAGAAGCAAGAAAGTACATTTTCAACTGTT TAGATGATATGGCCCAGGTGAACATGACGACAGATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTCATTGACC TGTTGAAGAAGATGCTGACCATTGATGCTGACAAGAGAATCACTCCAATCGAAACCCTGAACCATCCCTTTGTCACCATGACACACTTAC TCGATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATCTGCAAGCGTCGGGTGAATATGTATGACACGGTGAACC AGAGCAAAACCCCTTTCATCACGCACGTGGCCCCCAGCACGTCCACCAACCTGACCATGACCTTTAACAACCAGCTGACCACTGTCCACA ACCAGCCCTCAGCGGCATCCATGGCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGATTTGTGCCCGGCCTGACC CGTTCCAGCAAGCTCTCATCGTGTGTCCCCCCGGCTTCCAAGGCTTGCAGGCCTCTCCCTCTAAGCACGCTGGCTACTCGGTGCGAATGG AAAATGCAGTTCCCATCGTCACTCAAGCCCCAGGAGCTCAGCCTCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTG GGACCCAGCAGATCCTGCTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCCACCCACACATCAGTGCAGCATGCCACCGTGATTCCCG AGACCATGGCAGGCACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGCCATTATAATCCCATCATGCAGCAGCCTGCAC TATTGACCGGTCATGTGACCCTTCCAGCAGCACAGCCCTTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCAGCCAACCAGCACCACCT CCTCCCGGAAGAGTAAGCAGCACCAGTCATCTGTGAGAAATGTCTCCACCTGTGAGGTGTCCTCCTCTCAGGCCATCAGCTCCCCACAGC GATCCAAGCGTGTCAAGGAGAACACACCTCCCCGCTGTGCCATGGTGCACAGTAGCCCGGCCTGCAGCACCTCGGTCACCTGTGGGTGGG GCGACGTGGCCTCCAGCACCACCCGGGAACGGCAGCGGCAGACAATTGTCATTCCCGACACTCCCAGCCCCACGGTCAGCGTCATCACCA TCAGCAGTGACACGGACGAGGAGGAGGAACAGAAACACGCCCCCACCAGCACTGTCTCCAAGCAAAGAAAAAACGTCATCAGCTGTGTCA CAGTCCACGACTCCCCCTACTCCGACTCCTCCAGCAACACCAGCCCCTACTCCGTGCAGCAGCGTGCTGGGCACAACAATGCCAATGCCT TTGACACCAAGGGGAGCCTGGAGAATCACTGCACGGGGAACCCCCGAACCATCATCGTGCCACCCCTGAAAACCCAGGCCAGCGAAGTAT TGGTGGAGTGTGATAGCCTGGTGCCAGTCAACACCAGTCACCACTCGTCCTCCTACAAGTCCAAGTCCTCCAGCAACGTGACCTCCACCA GCGGTCACTCTTCAGGGAGCTCATCTGGAGCCATCACCTACCGGCAGCAGCGGCCGGGCCCCCACTTCCAGCAGCAGCAGCCACTCAATC TCAGCCAGGCTCAGCAGCACATCACCACGGACCGCACTGGGAGCCACCGAAGGCAGCAGGCCTACATCACTCCCACCATGGCCCAGGCTC CGTACTCCTTCCCGCACAACAGCCCCAGCCACGGCACTGTGCACCCGCATCTGGCTGCAGCCGCTGCCGCTGCCCACCTCCCCACCCAGC CCCACCTCTACACCTACACTGCGCCGGCGGCCCTGGGCTCCACCGGCACCGTGGCCCACCTGGTGGCCTCGCAAGGCTCTGCGCGCCACA CCGTGCAGCACACTGCCTACCCAGCCAGCATCGTCCACCAGGTCCCCGTGAGCATGGGCCCCCGGGTCCTGCCCTCGCCCACCATCCACC CGAGTCAGTATCCAGCCCAATTTGCCCACCAGACCTACATCAGCGCCTCGCCAGCCTCCACCGTCTACACTGGATACCCACTGAGCCCCG CCAAGGTCAACCAGTACCCTTACATATAAACACTGGAGGGGAGGGAGGGAGGGAGGGAGGGAGAGAATGGCCCGAGGGAGGAGGGAGAGA AGGAGGGAGGCGCTCCTGGGACCGTGGGCGCTGGCCTTTTATACTGAAGATGCCGCACACAAACAATGCAAACGGGGCAGGGGCGGGGGG GGGGGGGGGGGCAGAGGGCAGGGGGACGGGTCGGGACACCAGTGAAACTTGAACCGGGAAGTGGGAGGACGTAGAGCAGAGAAGAGAACA >66598_66598_3_PM20D2-HIPK2_PM20D2_chr6_89856328_ENST00000275072_HIPK2_chr7_139313785_ENST00000428878_length(amino acids)=907AA_BP=172 MEASGRVRGRSLPAAEPGSERAQRAAGLGSMRPGGERPVEGGACNGRSELELLKLRSAECIDEAAERLGALSRAIWSQPELAYEEHHAHR VLTHFFEREPPAASWAVQPHYQLPTAFRAEWEPPEARAPSATPRPLHLGFLCEYDALPGIGHACGHNLIAEVGAAAALGVRGALEGLPRP PPPVKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLD FPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQPSAASMAAVAQRSMPLQTGTAQICARPDPF QQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGLLAQQAWPSGTQQILLPPAWQQLTGVATHTSVQHATVIPET MAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKSKQHQSSVRNVSTCEVSSSQAISSPQRS KRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTTRERQRQTIVIPDTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQRKNVISCVTV HDSPYSDSSSNTSPYSVQQRAGHNNANAFDTKGSLENHCTGNPRTIIVPPLKTQASEVLVECDSLVPVNTSHHSSSYKSKSSSNVTSTSG HSSGSSSGAITYRQQRPGPHFQQQQPLNLSQAQQHITTDRTGSHRRQQAYITPTMAQAPYSFPHNSPSHGTVHPHLAAAAAAAHLPTQPH LYTYTAPAALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQVPVSMGPRVLPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLSPAK -------------------------------------------------------------- |
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Fusion Gene PPI Analysis for PM20D2-HIPK2 |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160) |
| Hgene | Hgene's interactors | Tgene | Tgene's interactors |
| - Retained PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 935_1049 | 449.0 | 1199.0 | AXIN1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 935_1049 | 449.0 | 1172.0 | AXIN1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 774_876 | 449.0 | 1199.0 | CTBP1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 774_876 | 449.0 | 1172.0 | CTBP1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 787_897 | 449.0 | 1199.0 | HMGA1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 787_897 | 449.0 | 1172.0 | HMGA1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 752_897 | 449.0 | 1199.0 | POU4F1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 752_897 | 449.0 | 1172.0 | POU4F1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 539_844 | 449.0 | 1199.0 | SKI and SMAD1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 539_844 | 449.0 | 1172.0 | SKI and SMAD1 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 846_941 | 449.0 | 1199.0 | TP53 and TP73 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 846_941 | 449.0 | 1172.0 | TP53 and TP73 | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 873_907 | 449.0 | 1199.0 | UBE2I | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 873_907 | 449.0 | 1172.0 | UBE2I |
| - Lost PPIs in in-frame fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000406875 | 3 | 15 | 189_520 | 449.0 | 1199.0 | DAXX | |
| Tgene | HIPK2 | chr6:89856328 | chr7:139313785 | ENST00000428878 | 3 | 15 | 189_520 | 449.0 | 1172.0 | DAXX |
| - Retained PPIs, but lost function due to frame-shift fusion. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs for PM20D2-HIPK2 |
| Drugs targeting genes involved in this fusion gene. (DrugBank Version 5.1.8 2021-05-08) |
| Partner | Gene | UniProtAcc | DrugBank ID | Drug name | Drug activity | Drug type | Drug status |
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Related Diseases for PM20D2-HIPK2 |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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