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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:RPH3AL-ALOX15B (FusionGDB2 ID:75734)

Fusion Gene Summary for RPH3AL-ALOX15B

check button Fusion gene summary
Fusion gene informationFusion gene name: RPH3AL-ALOX15B
Fusion gene ID: 75734
HgeneTgene
Gene symbol

RPH3AL

ALOX15B

Gene ID

9501

247

Gene namerabphilin 3A like (without C2 domains)arachidonate 15-lipoxygenase type B
SynonymsNOC215-LOX-2
Cytomap

17p13.3

17p13.1

Type of geneprotein-codingprotein-coding
Descriptionrab effector Noc2no C2 domains proteinarachidonate 15-lipoxygenase B15-LOX-B15S-lipoxygenasearachidonate 15-lipoxygenase 2arachidonate 15-lipoxygenase type IIarachidonate 15-lipoxygenase, second typearachidonate omega(6) lipoxygenaselinoleate 13-lipoxygenase 15-LOb
Modification date2020031320200320
UniProtAcc.

O15296

Ensembl transtripts involved in fusion geneENST00000576001, ENST00000323434, 
ENST00000331302, ENST00000536489, 
ENST00000380173, ENST00000380183, 
ENST00000572022, ENST00000573359, 
Fusion gene scores* DoF score8 X 9 X 7=5044 X 5 X 4=80
# samples 106
** MAII scorelog2(10/504*10)=-2.33342373372519
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/80*10)=-0.415037499278844
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: RPH3AL [Title/Abstract] AND ALOX15B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointRPH3AL(169211)-ALOX15B(7942704), # samples:2
Anticipated loss of major functional domain due to fusion event.RPH3AL-ALOX15B seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
RPH3AL-ALOX15B seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
RPH3AL-ALOX15B seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
RPH3AL-ALOX15B seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneALOX15B

GO:0008285

negative regulation of cell proliferation

11839751

TgeneALOX15B

GO:0019369

arachidonic acid metabolic process

9177185|10542053|10625675|24497644

TgeneALOX15B

GO:0045786

negative regulation of cell cycle

11839751

TgeneALOX15B

GO:0045926

negative regulation of growth

11839751


check buttonFusion gene breakpoints across RPH3AL (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across ALOX15B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4COADTCGA-AA-3662-01ARPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
ChimerDB4COADTCGA-AA-3662RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+


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Fusion Gene ORF analysis for RPH3AL-ALOX15B

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5UTR-3CDSENST00000576001ENST00000380173RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
5UTR-3CDSENST00000576001ENST00000380173RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
5UTR-3CDSENST00000576001ENST00000380183RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
5UTR-3CDSENST00000576001ENST00000380183RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
5UTR-3CDSENST00000576001ENST00000572022RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
5UTR-3CDSENST00000576001ENST00000572022RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
5UTR-3CDSENST00000576001ENST00000573359RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
5UTR-3CDSENST00000576001ENST00000573359RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000323434ENST00000380173RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000323434ENST00000380173RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000323434ENST00000380183RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000323434ENST00000380183RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000323434ENST00000572022RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000323434ENST00000572022RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000323434ENST00000573359RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000323434ENST00000573359RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000331302ENST00000380173RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000331302ENST00000380173RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000331302ENST00000380183RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000331302ENST00000380183RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000331302ENST00000572022RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000331302ENST00000572022RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000331302ENST00000573359RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000331302ENST00000573359RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000536489ENST00000380173RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000536489ENST00000380173RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000536489ENST00000380183RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000536489ENST00000380183RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000536489ENST00000572022RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000536489ENST00000572022RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+
Frame-shiftENST00000536489ENST00000573359RPH3ALchr17

169211

-ALOX15Bchr17

7942704

+
Frame-shiftENST00000536489ENST00000573359RPH3ALchr17

169210

-ALOX15Bchr17

7942703

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Genomic Features for RPH3AL-ALOX15B


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
RPH3ALchr17169210-ALOX15Bchr177942703+5.00E-050.99995005
RPH3ALchr17169210-ALOX15Bchr177942703+5.00E-050.99995005
RPH3ALchr17169210-ALOX15Bchr177942703+5.00E-050.99995005
RPH3ALchr17169210-ALOX15Bchr177942703+5.00E-050.99995005

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for RPH3AL-ALOX15B


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:169211/:7942704)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.ALOX15B

O15296

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: [Isoform A]: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (PubMed:9177185, PubMed:10625675, PubMed:12704195, PubMed:17493578, PubMed:18311922, PubMed:24282679, PubMed:10542053, PubMed:24497644, PubMed:32404334) (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (PubMed:17493578, PubMed:12704195, PubMed:24282679, PubMed:9177185, PubMed:11956198, PubMed:10625675, PubMed:24497644) (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed:10542053, PubMed:27435673). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed:18311922, PubMed:17493578, PubMed:11956198). Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates (PubMed:27145229). Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed:27435673). Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation (PubMed:12704195, PubMed:11839751). May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed:18067895). May also regulate macrophage differentiation into proatherogenic foam cells (PubMed:22912809). {ECO:0000269|PubMed:10542053, ECO:0000269|PubMed:10625675, ECO:0000269|PubMed:11839751, ECO:0000269|PubMed:11956198, ECO:0000269|PubMed:12704195, ECO:0000269|PubMed:17493578, ECO:0000269|PubMed:18067895, ECO:0000269|PubMed:18311922, ECO:0000269|PubMed:22912809, ECO:0000269|PubMed:24282679, ECO:0000269|PubMed:24497644, ECO:0000269|PubMed:27145229, ECO:0000269|PubMed:27435673, ECO:0000269|PubMed:32404334, ECO:0000269|PubMed:9177185, ECO:0000305|PubMed:10542053, ECO:0000305|PubMed:16112079, ECO:0000305|PubMed:27145229, ECO:0000305|PubMed:27435673}.; FUNCTION: [Isoform B]: Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. {ECO:0000269|PubMed:12704195}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for RPH3AL-ALOX15B


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

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Fusion Gene PPI Analysis for RPH3AL-ALOX15B


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for RPH3AL-ALOX15B


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for RPH3AL-ALOX15B


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource