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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:RPL36-CYBA (FusionGDB2 ID:76621)

Fusion Gene Summary for RPL36-CYBA

check button Fusion gene summary
Fusion gene informationFusion gene name: RPL36-CYBA
Fusion gene ID: 76621
HgeneTgene
Gene symbol

RPL36

CYBA

Gene ID

25873

1535

Gene nameribosomal protein L36cytochrome b-245 alpha chain
SynonymsL36p22-PHOX
Cytomap

19p13.3

16q24.2

Type of geneprotein-codingprotein-coding
Description60S ribosomal protein L36large ribosomal subunit protein eL36cytochrome b-245 light chaincytochrome b light chaincytochrome b(558) alpha chaincytochrome b(558) alpha-subunitcytochrome b, alpha polypeptidecytochrome b-245, alpha polypeptidecytochrome b558 subunit alphaflavocytochrome b-558 alpha polypeptiden
Modification date2020031320200329
UniProtAcc.

P13498

Ensembl transtripts involved in fusion geneENST00000579446, ENST00000347512, 
ENST00000394580, ENST00000577222, 
ENST00000579649, ENST00000582380, 
ENST00000261623, ENST00000561972, 
ENST00000567174, ENST00000569359, 
Fusion gene scores* DoF score20 X 9 X 5=9005 X 5 X 5=125
# samples 215
** MAII scorelog2(21/900*10)=-2.09953567355091
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(5/125*10)=-1.32192809488736
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: RPL36 [Title/Abstract] AND CYBA [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointRPL36(5691595)-CYBA(88709927), # samples:1
Anticipated loss of major functional domain due to fusion event.RPL36-CYBA seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneRPL36

GO:0002181

cytoplasmic translation

25957688

TgeneCYBA

GO:0017004

cytochrome complex assembly

7938008

TgeneCYBA

GO:0032755

positive regulation of interleukin-6 production

22423966

TgeneCYBA

GO:0032760

positive regulation of tumor necrosis factor production

22423966

TgeneCYBA

GO:0034137

positive regulation of toll-like receptor 2 signaling pathway

22423966

TgeneCYBA

GO:0050766

positive regulation of phagocytosis

22423966

TgeneCYBA

GO:1900426

positive regulation of defense response to bacterium

22423966

TgeneCYBA

GO:1903428

positive regulation of reactive oxygen species biosynthetic process

22423966


check buttonFusion gene breakpoints across RPL36 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across CYBA (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4READTCGA-AG-A008RPL36chr19

5691595

+CYBAchr16

88709927

-


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Fusion Gene ORF analysis for RPL36-CYBA

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000579446ENST00000261623RPL36chr19

5691595

+CYBAchr16

88709927

-
3UTR-intronENST00000579446ENST00000561972RPL36chr19

5691595

+CYBAchr16

88709927

-
3UTR-intronENST00000579446ENST00000567174RPL36chr19

5691595

+CYBAchr16

88709927

-
3UTR-intronENST00000579446ENST00000569359RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000347512ENST00000561972RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000347512ENST00000567174RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000347512ENST00000569359RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000394580ENST00000561972RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000394580ENST00000567174RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000394580ENST00000569359RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000577222ENST00000561972RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000577222ENST00000567174RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000577222ENST00000569359RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000579649ENST00000561972RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000579649ENST00000567174RPL36chr19

5691595

+CYBAchr16

88709927

-
5CDS-intronENST00000579649ENST00000569359RPL36chr19

5691595

+CYBAchr16

88709927

-
Frame-shiftENST00000394580ENST00000261623RPL36chr19

5691595

+CYBAchr16

88709927

-
Frame-shiftENST00000579649ENST00000261623RPL36chr19

5691595

+CYBAchr16

88709927

-
In-frameENST00000347512ENST00000261623RPL36chr19

5691595

+CYBAchr16

88709927

-
In-frameENST00000577222ENST00000261623RPL36chr19

5691595

+CYBAchr16

88709927

-
intron-3CDSENST00000582380ENST00000261623RPL36chr19

5691595

+CYBAchr16

88709927

-
intron-intronENST00000582380ENST00000561972RPL36chr19

5691595

+CYBAchr16

88709927

-
intron-intronENST00000582380ENST00000567174RPL36chr19

5691595

+CYBAchr16

88709927

-
intron-intronENST00000582380ENST00000569359RPL36chr19

5691595

+CYBAchr16

88709927

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000577222RPL36chr195691595+ENST00000261623CYBAchr1688709927-10588211040555161
ENST00000347512RPL36chr195691595+ENST00000261623CYBAchr1688709927-891654618145157

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000577222ENST00000261623RPL36chr195691595+CYBAchr1688709927-0.124876750.87512326
ENST00000347512ENST00000261623RPL36chr195691595+CYBAchr1688709927-0.212483610.7875164

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Fusion Genomic Features for RPL36-CYBA


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for RPL36-CYBA


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:5691595/chr16:88709927)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.CYBA

P13498

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. {ECO:0000269|PubMed:15824103}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneCYBAchr19:5691595chr16:88709927ENST0000026162306133_1890196.0Compositional biasNote=Pro-rich
TgeneCYBAchr19:5691595chr16:88709927ENST000002616230691_1270196.0Intramembrane.

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


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Fusion Gene Sequence for RPL36-CYBA


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>76621_76621_1_RPL36-CYBA_RPL36_chr19_5691595_ENST00000347512_CYBA_chr16_88709927_ENST00000261623_length(transcript)=891nt_BP=654nt
GAAGACTCCAGCCGCCAGGGGGAGCGCGTGCCGTTCTTGCCTCTCTGGCCTGCGCCTCCTGAGCCGAGTAGATATCCCGGAGTTCCGCGC
GGCGCCAGCCCTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTACCCTATGGCCGTGGGCCTCAACAAGGGCCACA
AAGTGACCAAGAACGTGAGCAAGCCCAGGCACAGCCGACGCCGCGGGCGTCTGACCAAACACACCAAGTTCGTGCGGGACATGATTCGGG
AGGTGTGTGGCTTTGCCCCGTACGAGCGGCGCGCCATGGAGTTACTGAAGGTCTCCAAGGACAAACGGGCCCTCAAATTTATCAAGAAAA
GGGGCCGCCATGAGGAAAGCTGCTGCCAAGAAAGACTGAGCCCCTCCCCTGCCCTCTCCCTGAAATAAAGAACAGCTTGACAGAAGCCCT
GGCTCTCCTGCTGTCCGTGGGTGGGTGTGGGTGTGTCGGGGGCCCGCAGTCCCCTGTCTGGTGCCCGCTCTGAGCCACACCCTCTCCGGG
TGCTGCCTGGTCGTGAATCAAAAGCCGTGGCCCGCCCACCCTTCCCGGGGCAGCAGGTGAGGAAGCCGCCGTACTGCAAATGACTTTAAT
CATTAAATAGCTTCTATGCCACACGGCCGCAGATCGGAGGCACCATCAAGCAGCCGCCCAGCAACCCCCCGCCGCGGCCCCCGGCCGAGG
CCCGCAAGAAGCCCAGCGAGGAGGAGGCTGCGGTGGCGGCGGGGGGACCCCCGGGAGGTCCCCAGGTCAACCCCATCCCGGTGACCGACG

>76621_76621_1_RPL36-CYBA_RPL36_chr19_5691595_ENST00000347512_CYBA_chr16_88709927_ENST00000261623_length(amino acids)=157AA_BP=
MQYGGFLTCCPGKGGRATAFDSRPGSTRRGCGSERAPDRGLRAPDTPTPTHGQQESQGFCQAVLYFRERAGEGLSLSWQQLSSWRPLFLI

--------------------------------------------------------------
>76621_76621_2_RPL36-CYBA_RPL36_chr19_5691595_ENST00000577222_CYBA_chr16_88709927_ENST00000261623_length(transcript)=1058nt_BP=821nt
CGAGAAGAGAGGACCGTGGGGGCGTTCGCGTGGCTCCCAGCCCGGGACCCCACCCCCGCTGGACAGTGGGGGAAACTGAGGCCTGAGCGG
AGTGACCACCCCGGCTATGGCCAGCCAGCCTCCAGGGAGCGCGAGTGCTGCTGACAGGGGCCAACGCTGGTGTTGGTGAGGAGCTGGCCT
ATCACTACGCGCGTCTGGGCTCCCACCTGGTGCTCACTGCCCACACTGAGGCTCTCCTGCAGAAGGGTCGGCCCGAAATAGGCGGAGTTG
GGATTCGAACTCATTTCACAGGCAGAGGGAGGAGAGCAGCCATAGCCACCTCCACTGGTTCCGACTCCAGCTCCCGGGCTCGCAGGAGTA
AACGCGCATGCTCAGGGAGCTCGGTCGCGCATGCTCAGCGCAACGGCGGGGAAGACTCCAGCCGCCAGGGGGAGCGCGTGCCGTTCTTGC
CTCTCTGGCCTGCGCCTCCTGAGCCGAGTAGATATCCCGGAGTTCCGCGCGGCGCCAGCCCTTCCGCCACGGCCGTCTCTGGAGAGCAGC
AGCCATGGCCCTACGCTACCCTATGGCCGTGGGCCTCAACAAGGGCCACAAAGTGACCAAGAACGTGAGCAAGCCCAGGCACAGCCGACG
CCGCGGGCGTCTGACCAAACACACCAAGTTCGTGCGGGACATGATTCGGGAGGTGTGTGGCTTTGCCCCGTACGAGCGGCGCGCCATGGA
GTTACTGAAGGTCTCCAAGGACAAACGGGCCCTCAAATTTATCAAGAAAAGGGGCCGCCATGAGGAAAGCTGCTGCCAAGAAAGACTGAG
CCCCTCCCCTGGGCCGCAGATCGGAGGCACCATCAAGCAGCCGCCCAGCAACCCCCCGCCGCGGCCCCCGGCCGAGGCCCGCAAGAAGCC
CAGCGAGGAGGAGGCTGCGGTGGCGGCGGGGGGACCCCCGGGAGGTCCCCAGGTCAACCCCATCCCGGTGACCGACGAGGTCGTGTGACC

>76621_76621_2_RPL36-CYBA_RPL36_chr19_5691595_ENST00000577222_CYBA_chr16_88709927_ENST00000261623_length(amino acids)=161AA_BP=1
MHLLQVGAPGGRAGPGRGHTTSSVTGMGLTWGPPGGPPAATAASSSLGFLRASAGGRGGGLLGGCLMVPPICGPGEGLSLSWQQLSSWRP

--------------------------------------------------------------

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Fusion Gene PPI Analysis for RPL36-CYBA


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for RPL36-CYBA


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for RPL36-CYBA


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource