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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
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Fusion gene:RUNX1-USP25 (FusionGDB2 ID:78696)

Fusion Gene Summary for RUNX1-USP25

Fusion gene summary

Fusion gene information	Fusion gene name: RUNX1-USP25
	Fusion gene ID: 78696
		Hgene	Tgene
	Gene symbol	RUNX1	USP25
	Gene ID	861	29761
	Gene name	RUNX family transcription factor 1	ubiquitin specific peptidase 25
	Synonyms	AML1\|AML1-EVI-1\|AMLCR1\|CBF2alpha\|CBFA2\|EVI-1\|PEBP2aB\|PEBP2alpha	USP21
	Cytomap	21q22.12	21q21.1
	Type of gene	protein-coding	protein-coding
	Description	runt-related transcription factor 1AML1-EVI-1 fusion proteinPEA2-alpha BPEBP2-alpha BSL3-3 enhancer factor 1 alpha B subunitSL3/AKV core-binding factor alpha B subunitacute myeloid leukemia 1 proteincore-binding factor, runt domain, alpha subunit 2	ubiquitin carboxyl-terminal hydrolase 25USP on chromosome 21deubiquitinating enzyme 25ubiquitin thioesterase 25ubiquitin thiolesterase 25ubiquitin-specific processing protease 25
	Modification date	20200322	20200313
	UniProtAcc	.	.
	Ensembl transtripts involved in fusion gene	ENST00000300305, ENST00000325074, ENST00000344691, ENST00000358356, ENST00000399240, ENST00000437180, ENST00000486278, ENST00000494829,	ENST00000547201, ENST00000285679, ENST00000285681, ENST00000351097, ENST00000400183,
Fusion gene scores	* DoF score	46 X 68 X 13=40664	11 X 13 X 6=858
	# samples	89	13
	** MAII score	log2(89/40664*10)=-5.51380298959468 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(13/858*10)=-2.72246602447109 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: RUNX1 [Title/Abstract] AND USP25 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	RUNX1(36231771)-USP25(17163821), # samples:1
Anticipated loss of major functional domain due to fusion event.	RUNX1-USP25 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. RUNX1-USP25 seems lost the major protein functional domain in Hgene partner, which is a transcription factor due to the frame-shifted ORF. RUNX1-USP25 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF. RUNX1-USP25 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	RUNX1	GO:0030097	hemopoiesis	21873977
Hgene	RUNX1	GO:0045893	positive regulation of transcription, DNA-templated	10207087\|14970218
Hgene	RUNX1	GO:0045944	positive regulation of transcription by RNA polymerase II	9199349\|10207087\|14970218\|21873977
Tgene	USP25	GO:0016579	protein deubiquitination	22590560

Fusion gene breakpoints across RUNX1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across USP25 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChiTaRS5.0	N/A	AF419247	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+

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Fusion Gene ORF analysis for RUNX1-USP25

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-3UTR	ENST00000300305	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
5CDS-3UTR	ENST00000325074	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
5CDS-3UTR	ENST00000344691	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
5CDS-3UTR	ENST00000358356	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
5CDS-3UTR	ENST00000399240	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
5CDS-3UTR	ENST00000437180	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
5CDS-3UTR	ENST00000486278	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000300305	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000300305	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000300305	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000300305	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000325074	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000325074	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000325074	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000325074	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000344691	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000344691	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000344691	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000344691	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000358356	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000358356	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000358356	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000358356	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000399240	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000399240	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000399240	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000399240	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000486278	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000486278	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000486278	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
Frame-shift	ENST00000486278	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
In-frame	ENST00000437180	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
In-frame	ENST00000437180	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
In-frame	ENST00000437180	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
In-frame	ENST00000437180	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
intron-3CDS	ENST00000494829	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
intron-3CDS	ENST00000494829	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
intron-3CDS	ENST00000494829	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
intron-3CDS	ENST00000494829	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+
intron-3UTR	ENST00000494829	ENST00000547201	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000437180	RUNX1	chr21	36231771	-	ENST00000351097	USP25	chr21	17163821	+	2200	803	840	1763	307
ENST00000437180	RUNX1	chr21	36231771	-	ENST00000285679	USP25	chr21	17163821	+	4015	803	840	3578	912
ENST00000437180	RUNX1	chr21	36231771	-	ENST00000285681	USP25	chr21	17163821	+	5258	803	840	3674	944
ENST00000437180	RUNX1	chr21	36231771	-	ENST00000400183	USP25	chr21	17163821	+	5372	803	840	3788	982

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000437180	ENST00000351097	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+	0.000783488	0.99921644
ENST00000437180	ENST00000285679	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+	0.000718302	0.9992817
ENST00000437180	ENST00000285681	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+	0.000285999	0.99971396
ENST00000437180	ENST00000400183	RUNX1	chr21	36231771	-	USP25	chr21	17163821	+	0.000314008	0.999686

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Fusion Genomic Features for RUNX1-USP25

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	1-p	p (fusion gene breakpoint)
RUNX1	chr21	36231770	-	USP25	chr21	17163820	+	9.79E-05	0.9999021
RUNX1	chr21	36231770	-	USP25	chr21	17163820	+	9.79E-05	0.9999021

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for RUNX1-USP25

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr21:36231771/chr21:17163821)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.	FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000300305

50_178

204

481.0

Domain

Runt

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000325074

50_178

192

469.0

Domain

Runt

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000344691

50_178

177

454.0

Domain

Runt

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000358356

50_178

177

251.0

Domain

Runt

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000437180

50_178

204

481.0

Domain

Runt

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285679

541_578

130

1056.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285679

684_717

130

1056.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285681

541_578

130

1088.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285681

684_717

130

1088.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000400183

541_578

130

1126.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000400183

684_717

130

1126.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285679

169_657

130

1056.0

Domain

Note=USP

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285681

169_657

130

1088.0

Domain

Note=USP

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000400183

169_657

130

1126.0

Domain

Note=USP

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000300305

187_453

204

481.0

Compositional bias

Note=Pro/Ser/Thr-rich

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000325074

187_453

192

469.0

Compositional bias

Note=Pro/Ser/Thr-rich

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000344691

187_453

177

454.0

Compositional bias

Note=Pro/Ser/Thr-rich

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000358356

187_453

177

251.0

Compositional bias

Note=Pro/Ser/Thr-rich

Hgene

RUNX1

chr21:36231771

chr21:17163821

ENST00000437180

187_453

204

481.0

Compositional bias

Note=Pro/Ser/Thr-rich

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285679

123_140

130

1056.0

Domain

UIM 2

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285679

14_57

130

1056.0

Domain

Note=UBA-like

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285679

97_116

130

1056.0

Domain

UIM 1

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285681

123_140

130

1088.0

Domain

UIM 2

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285681

14_57

130

1088.0

Domain

Note=UBA-like

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285681

97_116

130

1088.0

Domain

UIM 1

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000400183

123_140

130

1126.0

Domain

UIM 2

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000400183

14_57

130

1126.0

Domain

Note=UBA-like

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000400183

97_116

130

1126.0

Domain

UIM 1

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285679

89_95

130

1056.0

Motif

Note=Required for SUMO paralog-specific binding

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000285681

89_95

130

1088.0

Motif

Note=Required for SUMO paralog-specific binding

Tgene

USP25

chr21:36231771

chr21:17163821

ENST00000400183

89_95

130

1126.0

Motif

Note=Required for SUMO paralog-specific binding

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Fusion Gene Sequence for RUNX1-USP25

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>78696_78696_1_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000285679_length(transcript)=4015nt_BP=803nt
CTTTGGGCCTCATAAACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGTCTGAACCCAGCATAGTGGTCAGCAGGCAGG
ACGAATCACACTGAATGCAAACCACAGGGTTTCGCAGCGTGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCA
GGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCGTACCCACAGTGCTTCATGAGAGAATGCATACTTGGAATGAATCC
TTCTAGAGACGTCCACGATGCCAGCACGAGCCGCCGCTTCACGCCGCCTTCCACCGCGCTGAGCCCAGGCAAGATGAGCGAGGCGTTGCC
GCTGGGCGCCCCGGACGCCGGCGCTGCCCTGGCCGGCAAGCTGAGGAGCGGCGACCGCAGCATGGTGGAGGTGCTGGCCGACCACCCGGG
CGAGCTGGTGCGCACCGACAGCCCCAACTTCCTCTGCTCCGTGCTGCCTACGCACTGGCGCTGCAACAAGACCCTGCCCATCGCTTTCAA
GGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCACTGTGATGGCTGGCAATGATGAAAACTACTCGGCTGAGCTGAGAAATGC
TACCGCAGCCATGAAGAACCAGGTTGCAAGATTTAATGACCTCAGGTTTGTCGGTCGAAGTGGAAGAGGGAAAAGCTTCACTCTGACCAT
CACTGTCTTCACAAACCCACCGCAAGTCGCCACCTACCACAGAGCCATCAAAATCACAGTGGATGGGCCCCGAGAACCTCGAAAGTTCTT
GAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGA
CAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTTAGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTT
AGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGT
GAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTC
AAATCAAATGACTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAGCTGAA
GAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAA
TTTGAAAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAA
GGAGAAATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAA
TTGTCAAGATTTGAATTTAATCAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTTTTATATTTGGACAGA
TACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTA
GAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCT
GTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCATACCATCACAGACATTACCAAGCACAACAGAACAA
CAGGGAGCCCTATCTTCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAACCATTTACT
CAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATAACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACAT
CGCTGGAGGACAGAAATAGAAAATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACTCTGAC
AAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTT
GATCATCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGT
TATAGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAG
CCCCTTGTTGGTATAGAAACATTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTAGAAGAATGG
GATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAA
GCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAG
GCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCAATTAAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAA
GAAGACACCCCACCAGAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATTATTGAGAAA
ACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAAGCCAAACTGGAAATG
ATAAAACCTGAAGAAGTAAACTTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCATAATT
GGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAA
GGCTTATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCAGAACTCTTC
GAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAA
ATGGAAGAAAAGGATATACTAGCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACACCTCCAAGAA
AAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATTCCACG
CATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGATAAACTGCACACTTTCCCTGAACA
CACTGTATAAACTCTTTTTAGTTCTTAACCCTTGCCTTCCTGTCACAGGGTTTGCTTGTTGCTGCTATAGTTTTTAACTTTTTTTTATTT
TAATAACTGCAAAAGACAAAATGACTATACAGACTTTAGTCAGACTGCAGACAATAAAGCTGAAAATCGCATGGCGCTCAGACATTTTAA
CCGGAACTGATGTATAATCACAAATCTAATTGATTTTATTATGGCAAAACTATGCTTTTGCCACCTTCCTGTTGCAGTATTACTTTGCTT
TTATCTTTTCTTTCTCAACAGCTTTCCATTCAGTCTGGATCCTTCCATGACTACAGCCATTTAAGTGTTCAGCACTGTGTACGATACATA

>78696_78696_1_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000285679_length(amino acids)=912AA_BP=
MKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLV
GTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQY
PLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITR
IKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPS
TSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRL
HAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPP
DLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSP
ETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEE
YEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNN
GLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARI

--------------------------------------------------------------
>78696_78696_2_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000285681_length(transcript)=5258nt_BP=803nt
CTTTGGGCCTCATAAACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGTCTGAACCCAGCATAGTGGTCAGCAGGCAGG
ACGAATCACACTGAATGCAAACCACAGGGTTTCGCAGCGTGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCA
GGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCGTACCCACAGTGCTTCATGAGAGAATGCATACTTGGAATGAATCC
TTCTAGAGACGTCCACGATGCCAGCACGAGCCGCCGCTTCACGCCGCCTTCCACCGCGCTGAGCCCAGGCAAGATGAGCGAGGCGTTGCC
GCTGGGCGCCCCGGACGCCGGCGCTGCCCTGGCCGGCAAGCTGAGGAGCGGCGACCGCAGCATGGTGGAGGTGCTGGCCGACCACCCGGG
CGAGCTGGTGCGCACCGACAGCCCCAACTTCCTCTGCTCCGTGCTGCCTACGCACTGGCGCTGCAACAAGACCCTGCCCATCGCTTTCAA
GGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCACTGTGATGGCTGGCAATGATGAAAACTACTCGGCTGAGCTGAGAAATGC
TACCGCAGCCATGAAGAACCAGGTTGCAAGATTTAATGACCTCAGGTTTGTCGGTCGAAGTGGAAGAGGGAAAAGCTTCACTCTGACCAT
CACTGTCTTCACAAACCCACCGCAAGTCGCCACCTACCACAGAGCCATCAAAATCACAGTGGATGGGCCCCGAGAACCTCGAAAGTTCTT
GAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGA
CAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTTAGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTT
AGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGT
GAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTC
AAATCAAATGACTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAGCTGAA
GAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAA
TTTGAAAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAA
GGAGAAATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAA
TTGTCAAGATTTGAATTTAATCAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTTTTATATTTGGACAGA
TACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTA
GAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCT
GTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCATACCATCACAGACATTACCAAGCACAACAGAACAA
CAGGGAGCCCTATCTTCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAACCATTTACT
CAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATAACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACAT
CGCTGGAGGACAGAAATAGAAAATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACTCTGAC
AAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTT
GATCATCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGT
TATAGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAG
CCCCTTGTTGGTATAGAAACATTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTAGAAGAATGG
GATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAA
GCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAG
GCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGATCATGATGACACCGAACATGCAAGGTATTATCATGGCGATA
GGTAAATCCAGGAGTGTATATGACAGGTGTGGCCCTGAAGCAGGGTTCTTTAAGGCAATTAAGTTGGAATATGCAAGGTTGGTTAAGTTG
GCCCAAGAAGACACCCCACCAGAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATTATT
GAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAAGCCAAACTG
GAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTC
ATAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTG
TCTAAAGGCTTATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCAGAA
CTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATTTTTGCCATTATTACTGGTG
GATGAAATGGAAGAAAAGGATATACTAGCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACACCTC
CAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATAT
TCCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGATAAACTGCACACTTTCCC
TGAACACACTGTATAAACTCTTTTTAGTTCTTAACCCTTGCCTTCCTGTCACAGGGTTTGCTTGTTGCTGCTATAGTTTTTAACTTTTTT
TTATTTTAATAACTGCAAAAGACAAAATGACTATACAGACTTTAGTCAGACTGCAGACAATAAAGCTGAAAATCGCATGGCGCTCAGACA
TTTTAACCGGAACTGATGTATAATCACAAATCTAATTGATTTTATTATGGCAAAACTATGCTTTTGCCACCTTCCTGTTGCAGTATTACT
TTGCTTTTATCTTTTCTTTCTCAACAGCTTTCCATTCAGTCTGGATCCTTCCATGACTACAGCCATTTAAGTGTTCAGCACTGTGTACGA
TACATAATATTTGGTAGCTTGTAAATGAAATAAAGAATAAAGTTTTATTTATGGCTACCTATGTGTTTGTAAGCAGGTATATTGTATATT
AGTGTATTAGTAATACTAGATAAATGAATTTTGTCTGGGGATTAAGATTGGATAGTTAATAGATTAATACAATCTTTTAATTCTGCTCTA
ATGCTAGCAAATTGGAAAATGTTTAAGTCTTTGACACTTAAATTTATCTATATTTTTAACAAAGTTCTTGAACTTAGTATGGCACCGGAA
CCTGTTTTGAATTCAGTCAGGTTTTTACTCAAGTAAGTGGTTGATTTTTTTTAAGTCAAACTACACTGAAACTTTTATCCTTTTCTTAGA
TTAATCTTACTTTTTAAATGTATTTACAATATACAGCAAGGTGATTATTTCAAGAGAATCCCAAAGTACTTGAATAAGGGCTATTGTAAA
ATTTAAAAGAAATATTTATATATACACATATATACACATACACACATGTATATATATATTCTTCATAATGGAGGACAATGTTTTGCAATA
TATAAATCATTCTATTTTTGTAAATTGTATATCACTTTAATTGAAAATGTTCTCTACTAATTAATACTGTGAAACAAAATTGATGTTGTT
TAACTAGAAGTTATGAGTATCTTAACTGCCTTTATTCCTTTTCAAAAAGGAAAAAGCTGTAGAACATTTTGTAGATGAAACTACTGTTTA
AGATTAATGAATTAATATTGTGAATGAAAATCAAAATCCATACTTTAAAGGTAATCATGTTACTAACAACCTATTTTTGAATTCATAAAA
ATTTCTTTATAAATGATGTTTTGTGAACATAGTAAAATAGACCATTATACTATGTGTATGTTTGATACAGCGTCGCCAAAACTAGTGTTC
TTTATTAGTGCCTCTCACAAAAGATCCTGGATGGAGGAGTAAGATGAAATATTATGCTATTATATGATGCTGTTTGTAAAGGTATTAATG
TACTAGTAAGGTGTTAATGACAAGGAATTAGTACTATTCCTGTTGTAAAGTTAGATTTTGCATATTGTATCTATCAAAATATGTTTGGGT
TTAGATTTTAAGTTGTCTACTGAGCAGATTTCTGCATTGGTTTTCCAGTCCTGTTAAAAGTTTAGAAACTTCATATGTGTCATCACAGCT

>78696_78696_2_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000285681_length(amino acids)=944AA_BP=
MKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLV
GTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQY
PLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITR
IKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPS
TSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRL
HAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPP
DLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSP
ETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGD
RNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDE
ELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPK

--------------------------------------------------------------
>78696_78696_3_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000351097_length(transcript)=2200nt_BP=803nt
CTTTGGGCCTCATAAACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGTCTGAACCCAGCATAGTGGTCAGCAGGCAGG
ACGAATCACACTGAATGCAAACCACAGGGTTTCGCAGCGTGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCA
GGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCGTACCCACAGTGCTTCATGAGAGAATGCATACTTGGAATGAATCC
TTCTAGAGACGTCCACGATGCCAGCACGAGCCGCCGCTTCACGCCGCCTTCCACCGCGCTGAGCCCAGGCAAGATGAGCGAGGCGTTGCC
GCTGGGCGCCCCGGACGCCGGCGCTGCCCTGGCCGGCAAGCTGAGGAGCGGCGACCGCAGCATGGTGGAGGTGCTGGCCGACCACCCGGG
CGAGCTGGTGCGCACCGACAGCCCCAACTTCCTCTGCTCCGTGCTGCCTACGCACTGGCGCTGCAACAAGACCCTGCCCATCGCTTTCAA
GGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCACTGTGATGGCTGGCAATGATGAAAACTACTCGGCTGAGCTGAGAAATGC
TACCGCAGCCATGAAGAACCAGGTTGCAAGATTTAATGACCTCAGGTTTGTCGGTCGAAGTGGAAGAGGGAAAAGCTTCACTCTGACCAT
CACTGTCTTCACAAACCCACCGCAAGTCGCCACCTACCACAGAGCCATCAAAATCACAGTGGATGGGCCCCGAGAACCTCGAAAGTTCTT
GAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGA
CAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTTAGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTT
AGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGT
GAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTC
AAATCAAATGACTCACAGCAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCATAATTGGGCTAGAAAATTTT
CAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTTATACAGAGGA
CATGATGAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGAT
CGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGAT
ATACTAGCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACACCTCCAAGAAAAGCTGACAGATTTT
TTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATTCCACGCATGAACTCTGTGAG
CGATTTGCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGATAAACTGCACACTTTCCCTGAACACACTGTATAAACTCT
TTTTAGTTCTTAACCCTTGCCTTCCTGTCACAGGGTTTGCTTGTTGCTGCTATAGTTTTTAACTTTTTTTTATTTTAATAACTGCAAAAG
ACAAAATGACTATACAGACTTTAGTCAGACTGCAGACAATAAAGCTGAAAATCGCATGGCGCTCAGACATTTTAACCGGAACTGATGTAT
AATCACAAATCTAATTGATTTTATTATGGCAAAACTATGCTTTTGCCACCTTCCTGTTGCAGTATTACTTTGCTTTTATCTTTTCTTTCT
CAACAGCTTTCCATTCAGTCTGGATCCTTCCATGACTACAGCCATTTAAGTGTTCAGCACTGTGTACGATACATAATATTTGGTAGCTTG

>78696_78696_3_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000351097_length(amino acids)=307AA_BP=
MKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLV
GTKRKYVDPSRAVEILKDAFKSNDSQQEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYR
RECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSME

--------------------------------------------------------------
>78696_78696_4_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000400183_length(transcript)=5372nt_BP=803nt
CTTTGGGCCTCATAAACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGTCTGAACCCAGCATAGTGGTCAGCAGGCAGG
ACGAATCACACTGAATGCAAACCACAGGGTTTCGCAGCGTGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCA
GGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCGTACCCACAGTGCTTCATGAGAGAATGCATACTTGGAATGAATCC
TTCTAGAGACGTCCACGATGCCAGCACGAGCCGCCGCTTCACGCCGCCTTCCACCGCGCTGAGCCCAGGCAAGATGAGCGAGGCGTTGCC
GCTGGGCGCCCCGGACGCCGGCGCTGCCCTGGCCGGCAAGCTGAGGAGCGGCGACCGCAGCATGGTGGAGGTGCTGGCCGACCACCCGGG
CGAGCTGGTGCGCACCGACAGCCCCAACTTCCTCTGCTCCGTGCTGCCTACGCACTGGCGCTGCAACAAGACCCTGCCCATCGCTTTCAA
GGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCACTGTGATGGCTGGCAATGATGAAAACTACTCGGCTGAGCTGAGAAATGC
TACCGCAGCCATGAAGAACCAGGTTGCAAGATTTAATGACCTCAGGTTTGTCGGTCGAAGTGGAAGAGGGAAAAGCTTCACTCTGACCAT
CACTGTCTTCACAAACCCACCGCAAGTCGCCACCTACCACAGAGCCATCAAAATCACAGTGGATGGGCCCCGAGAACCTCGAAAGTTCTT
GAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGA
CAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTTAGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTT
AGAAGATTAGTTCTGAATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCTTTTATGCGT
GAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTC
AAATCAAATGACTCACAGCAGCAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAGCTGAA
GAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAA
TTTGAAAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAA
GGAGAAATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAA
TTGTCAAGATTTGAATTTAATCAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTTTTATATTTGGACAGA
TACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTA
GAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCT
GTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCATACCATCACAGACATTACCAAGCACAACAGAACAA
CAGGGAGCCCTATCTTCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAACCATTTACT
CAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATAACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACAT
CGCTGGAGGACAGAAATAGAAAATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACTCTGAC
AAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTT
GATCATCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGT
TATAGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAG
CCCCTTGTTGGTATAGAAACATTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTAGAAGAATGG
GATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAA
GCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAG
GCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGAAACCTGAAAATACTACAAGCCAACCACTTTCTAATCAGCGA
GTTGTAGAGGTGGCGATCCCTCATGTAGGGAAATTTATGATTGAATCAAAGGAGGGGGGGTATGATGACGAGATCATGATGACACCGAAC
ATGCAAGGTATTATCATGGCGATAGGTAAATCCAGGAGTGTATATGACAGGTGTGGCCCTGAAGCAGGGTTCTTTAAGGCAATTAAGTTG
GAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCAGAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAG
AACCAGGCACCAAAGAAAATTATTGAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGTCACAACATA
ATGAAAGTTGCTCAAGCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAA
TTCAGGGAAACAACTATGTATCTCATAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTCATCTGTGCT
TATCAGAATAACAAAGAACTCTTGTCTAAAGGCTTATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTA
AAATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTC
CCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTAGCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTT
GGTCAAGAAATGGAACCACACCTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAAAGTTTCCAT
GAGCCACCGAAGTTACCTTCATATTCCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGAT
GGAAGATAAACTGCACACTTTCCCTGAACACACTGTATAAACTCTTTTTAGTTCTTAACCCTTGCCTTCCTGTCACAGGGTTTGCTTGTT
GCTGCTATAGTTTTTAACTTTTTTTTATTTTAATAACTGCAAAAGACAAAATGACTATACAGACTTTAGTCAGACTGCAGACAATAAAGC
TGAAAATCGCATGGCGCTCAGACATTTTAACCGGAACTGATGTATAATCACAAATCTAATTGATTTTATTATGGCAAAACTATGCTTTTG
CCACCTTCCTGTTGCAGTATTACTTTGCTTTTATCTTTTCTTTCTCAACAGCTTTCCATTCAGTCTGGATCCTTCCATGACTACAGCCAT
TTAAGTGTTCAGCACTGTGTACGATACATAATATTTGGTAGCTTGTAAATGAAATAAAGAATAAAGTTTTATTTATGGCTACCTATGTGT
TTGTAAGCAGGTATATTGTATATTAGTGTATTAGTAATACTAGATAAATGAATTTTGTCTGGGGATTAAGATTGGATAGTTAATAGATTA
ATACAATCTTTTAATTCTGCTCTAATGCTAGCAAATTGGAAAATGTTTAAGTCTTTGACACTTAAATTTATCTATATTTTTAACAAAGTT
CTTGAACTTAGTATGGCACCGGAACCTGTTTTGAATTCAGTCAGGTTTTTACTCAAGTAAGTGGTTGATTTTTTTTAAGTCAAACTACAC
TGAAACTTTTATCCTTTTCTTAGATTAATCTTACTTTTTAAATGTATTTACAATATACAGCAAGGTGATTATTTCAAGAGAATCCCAAAG
TACTTGAATAAGGGCTATTGTAAAATTTAAAAGAAATATTTATATATACACATATATACACATACACACATGTATATATATATTCTTCAT
AATGGAGGACAATGTTTTGCAATATATAAATCATTCTATTTTTGTAAATTGTATATCACTTTAATTGAAAATGTTCTCTACTAATTAATA
CTGTGAAACAAAATTGATGTTGTTTAACTAGAAGTTATGAGTATCTTAACTGCCTTTATTCCTTTTCAAAAAGGAAAAAGCTGTAGAACA
TTTTGTAGATGAAACTACTGTTTAAGATTAATGAATTAATATTGTGAATGAAAATCAAAATCCATACTTTAAAGGTAATCATGTTACTAA
CAACCTATTTTTGAATTCATAAAAATTTCTTTATAAATGATGTTTTGTGAACATAGTAAAATAGACCATTATACTATGTGTATGTTTGAT
ACAGCGTCGCCAAAACTAGTGTTCTTTATTAGTGCCTCTCACAAAAGATCCTGGATGGAGGAGTAAGATGAAATATTATGCTATTATATG
ATGCTGTTTGTAAAGGTATTAATGTACTAGTAAGGTGTTAATGACAAGGAATTAGTACTATTCCTGTTGTAAAGTTAGATTTTGCATATT
GTATCTATCAAAATATGTTTGGGTTTAGATTTTAAGTTGTCTACTGAGCAGATTTCTGCATTGGTTTTCCAGTCCTGTTAAAAGTTTAGA

>78696_78696_4_RUNX1-USP25_RUNX1_chr21_36231771_ENST00000437180_USP25_chr21_17163821_ENST00000400183_length(amino acids)=982AA_BP=
MKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLV
GTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQY
PLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITR
IKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPS
TSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRL
HAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPP
DLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSP
ETVLQSKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFKAIKLEYARLVKLAQ
EDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLII
GLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDE

--------------------------------------------------------------

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Fusion Gene PPI Analysis for RUNX1-USP25

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Still interaction with
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000300305	-	5	8	135_143	204.33333333333334	481.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000300305	-	5	8	168_177	204.33333333333334	481.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000300305	-	5	8	80_84	204.33333333333334	481.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000325074	-	4	7	135_143	192.33333333333334	469.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000325074	-	4	7	168_177	192.33333333333334	469.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000325074	-	4	7	80_84	192.33333333333334	469.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000344691	-	3	6	135_143	177.33333333333334	454.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000344691	-	3	6	168_177	177.33333333333334	454.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000344691	-	3	6	80_84	177.33333333333334	454.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000358356	-	3	5	135_143	177.33333333333334	251.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000358356	-	3	5	168_177	177.33333333333334	251.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000358356	-	3	5	80_84	177.33333333333334	251.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000437180	-	6	9	135_143	204.33333333333334	481.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000437180	-	6	9	168_177	204.33333333333334	481.0	DNA
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000437180	-	6	9	80_84	204.33333333333334	481.0	DNA

- Lost PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000300305	-	5	8	362_402	204.33333333333334	481.0	FOXP3
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000325074	-	4	7	362_402	192.33333333333334	469.0	FOXP3
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000344691	-	3	6	362_402	177.33333333333334	454.0	FOXP3
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000358356	-	3	5	362_402	177.33333333333334	251.0	FOXP3
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000437180	-	6	9	362_402	204.33333333333334	481.0	FOXP3
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000300305	-	5	8	291_371	204.33333333333334	481.0	KAT6A
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000325074	-	4	7	291_371	192.33333333333334	469.0	KAT6A
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000344691	-	3	6	291_371	177.33333333333334	454.0	KAT6A
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000358356	-	3	5	291_371	177.33333333333334	251.0	KAT6A
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000437180	-	6	9	291_371	204.33333333333334	481.0	KAT6A
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000300305	-	5	8	307_400	204.33333333333334	481.0	KAT6B
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000325074	-	4	7	307_400	192.33333333333334	469.0	KAT6B
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000344691	-	3	6	307_400	177.33333333333334	454.0	KAT6B
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000358356	-	3	5	307_400	177.33333333333334	251.0	KAT6B
Hgene	RUNX1	chr21:36231771	chr21:17163821	ENST00000437180	-	6	9	307_400	204.33333333333334	481.0	KAT6B

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs for RUNX1-USP25

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

Top

Related Diseases for RUNX1-USP25

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source