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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:SLC35F2-PIWIL4 (FusionGDB2 ID:83013)

Fusion Gene Summary for SLC35F2-PIWIL4

check button Fusion gene summary
Fusion gene informationFusion gene name: SLC35F2-PIWIL4
Fusion gene ID: 83013
HgeneTgene
Gene symbol

SLC35F2

PIWIL4

Gene ID

54733

143689

Gene namesolute carrier family 35 member F2piwi like RNA-mediated gene silencing 4
SynonymsHSNOV1HIWI2|MIWI2
Cytomap

11q22.3

11q21

Type of geneprotein-codingprotein-coding
Descriptionsolute carrier family 35 member F2lung squamous cell cancer related protein LSCC-3piwi-like protein 4piwi-like 4testis tissue sperm-binding protein Li 85P
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000429869, ENST00000525071, 
ENST00000525815, ENST00000265836, 
ENST00000375682, 		ENST00000537419, 
ENST00000299001, 
Fusion gene scores* DoF score6 X 5 X 5=1502 X 1 X 2=4
# samples 52
** MAII scorelog2(5/150*10)=-1.58496250072116
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(2/4*10)=2.32192809488736
Context

PubMed: SLC35F2 [Title/Abstract] AND PIWIL4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointSLC35F2(107729384)-PIWIL4(94340605), # samples:2
Anticipated loss of major functional domain due to fusion event.SLC35F2-PIWIL4 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
SLC35F2-PIWIL4 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across SLC35F2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across PIWIL4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SKCMTCGA-D9-A4Z3-01ASLC35F2chr11

107729384

-PIWIL4chr11

94340605

+


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Fusion Gene ORF analysis for SLC35F2-PIWIL4

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000429869ENST00000537419SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
5CDS-intronENST00000525071ENST00000537419SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
5CDS-intronENST00000525815ENST00000537419SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
5UTR-3CDSENST00000265836ENST00000299001SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
5UTR-intronENST00000265836ENST00000537419SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
Frame-shiftENST00000429869ENST00000299001SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
Frame-shiftENST00000525071ENST00000299001SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
In-frameENST00000525815ENST00000299001SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
intron-3CDSENST00000375682ENST00000299001SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+
intron-intronENST00000375682ENST00000537419SLC35F2chr11

107729384

-PIWIL4chr11

94340605

+

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000525815SLC35F2chr11107729384-ENST00000299001PIWIL4chr1194340605+18815312371451404

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000525815ENST00000299001SLC35F2chr11107729384-PIWIL4chr1194340605+0.0010700740.9989299

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Fusion Genomic Features for SLC35F2-PIWIL4


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)
SLC35F2chr11107729383-PIWIL4chr1194340604+4.25E-050.99995744
SLC35F2chr11107729383-PIWIL4chr1194340604+4.25E-050.99995744

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.
genomic feature of top 1%

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Fusion Protein Features for SLC35F2-PIWIL4


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:107729384/chr11:94340605)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgenePIWIL4chr11:107729384chr11:94340605ENST000002990011220546_838546853.0DomainPiwi

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18108_1260328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18136_1560328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18165_1850328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18195_2150328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18227_2470328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18263_2830328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18291_3110328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-18314_3340328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-1839_590328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000375682-1873_930328.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411108_12636375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411136_15636375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411165_18536375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411195_21536375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411227_24736375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411263_28336375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411291_31136375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-411314_33436375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-41139_5936375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000429869-41173_9336375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411108_12636358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411136_15636358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411165_18536358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411195_21536358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411227_24736358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411263_28336358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411291_31136358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-411314_33436358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-41139_5936358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525071-41173_9336358.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18108_12636375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18136_15636375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18165_18536375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18195_21536375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18227_24736375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18263_28336375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18291_31136375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-18314_33436375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-1839_5936375.0TransmembraneHelical
HgeneSLC35F2chr11:107729384chr11:94340605ENST00000525815-1873_9336375.0TransmembraneHelical
TgenePIWIL4chr11:107729384chr11:94340605ENST000002990011220268_384546853.0DomainPAZ


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Fusion Gene Sequence for SLC35F2-PIWIL4


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>83013_83013_1_SLC35F2-PIWIL4_SLC35F2_chr11_107729384_ENST00000525815_PIWIL4_chr11_94340605_ENST00000299001_length(transcript)=1881nt_BP=531nt
ACTGCTTCTAACTCACTGCTCACTCACCGCTTCTTAGCTTAGGAATCACCTACAATAACCTCGTGTCACTGTATCTTGTGATTTCAAACA
AGGAACCCTAAAAACCTGAAGGCACACTGGGTGCCGCTCAACAATAAGGCTCGGCTGCCAAGACGAAAAAAAAATTGCCCTTTCTCCTCC
TGCCACCCTGGAGGTGCCAGGTTGTAATCTGCAAATGTATTGTCTGGCCCTTAGGGCTTGACAGTTAATGATTGGTCGCTGAAGGAGCCG
AGCGGAGGAGCGAGAGGGACGCGAGCAAGCGAGCGAGGGAGGGAGCGCAGGGCCAGAGCGGAGCCCCAGCCGGCCGCACAGGCACCGCCT
CCCGTCTTCCGGGACCCGGCACTTCGGGGGTTTTGGGCGCCGGAGACGCGCAAGGGCGCCGATGGAGGCAGACTCGCCAGCGGGCCCCGG
CGCCCCAGAGCCCCTCGCGGAGGGAGCGGCGGCCGAGTTCTCCAGCCTGCTGCGCAGGATAAAAGGCAAACTCTTCACCTGGTAATGTGC
ATTCTGCCTTCTAATCAGAAGACCTATTATGATTCCATTAAAAAATATTTGAGCTCAGACTGCCCAGTCCCAAGCCAATGTGTGCTTGCT
CGGACCTTGAATAAACAGGGCATGATGATGAGTATCGCCACCAAGATCGCTATGCAGATGACTTGCAAGCTCGGAGGCGAGCTGTGGGCT
GTGGAAATACCTTTAAAGTCCCTGATGGTGGTCGGTATTGATGTCTGTAAAGATGCACTCAGCAAGGACGTGATGGTTGTTGGATGCGTG
GCCAGTGTTAACCCCAGAATCACCAGGTGGTTTTCCCGCTGTATCCTTCAGAGAACAATGACTGATGTTGCAGATTGCTTGAAAGTTTTC
ATGACTGGAGCACTCAACAAATGGTACAAGTACAATCATGATTTGCCAGCACGGATAATTGTGTACCGTGCTGGTGTAGGGGATGGTCAG
CTGAAAACACTTATTGAATATGAAGTCCCACAGCTGCTGAGCAGTGTGGCAGAATCCAGCTCAAATACCAGCTCAAGACTGTCGGTGATT
GTGGTCAGGAAGAAGTGCATGCCACGATTCTTTACCGAAATGAACCGCACTGTACAGAACCCCCCACTTGGCACTGTTGTGGATTCAGAA
GCAACACGTAACGAATGGTATGACTTTTATCTGATCAGCCAGGTGGCCTGCCGGGGAACTGTTAGTCCTACCTACTATAATGTCATCTAT
GATGACAACGGCTTGAAGCCCGACCATATGCAGAGACTTACATTCAAATTGTGCCACCTGTACTACAACTGGCCGGGCATAGTCAGTGTC
CCAGCACCATGTCAGTATGCTCACAAGCTGACCTTTCTGGTGGCACAAAGCATTCATAAAGAACCCAGTCTGGAATTAGCCAACCATCTC
TTCTACCTGTGATGGCATGAACTACTGGCATCACTAGATGGACAATCCAAGAAGAAATTGGTATACTTTGTGCAAATCTGCCATAAGCTC
AAGGCTGTGACTGGGGAAAAAGATTGAGCTTAGTTTTCATGTCTAGGAAAAAAAGCAAAACAACTTAATCTGAAACAGTTTTAAAAAATG
TGTGTTATTTTGTTTTAAAGAGTTGTATGCTTGGGGTAAATTTTCATTGTCATATGTGGAATTTAAATATACCATCATCTACAAAGAATT
CCACAGAGTTAAATATCTTAAGTTAAACACTTAAAATAAGTGTTTGCGTGATATTTTGATGACAGATAAACAGAGTCTAATTCCCACCCC

>83013_83013_1_SLC35F2-PIWIL4_SLC35F2_chr11_107729384_ENST00000525815_PIWIL4_chr11_94340605_ENST00000299001_length(amino acids)=404AA_BP=98
MTVNDWSLKEPSGGARGTRASERGRERRARAEPQPAAQAPPPVFRDPALRGFWAPETRKGADGGRLASGPRRPRAPRGGSGGRVLQPAAQ
DKRQTLHLVMCILPSNQKTYYDSIKKYLSSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDVCKDA
LSKDVMVVGCVASVNPRITRWFSRCILQRTMTDVADCLKVFMTGALNKWYKYNHDLPARIIVYRAGVGDGQLKTLIEYEVPQLLSSVAES
SSNTSSRLSVIVVRKKCMPRFFTEMNRTVQNPPLGTVVDSEATRNEWYDFYLISQVACRGTVSPTYYNVIYDDNGLKPDHMQRLTFKLCH

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Fusion Gene PPI Analysis for SLC35F2-PIWIL4


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for SLC35F2-PIWIL4


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for SLC35F2-PIWIL4


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource