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Center for Computational Systems Medicine
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Fusion Gene Summary

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Fusion Gene ORF analysis

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Fusion Genomic Features

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Fusion Protein Features

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Fusion Gene Sequence

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Fusion Gene PPI analysis

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Related Drugs

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Related Diseases

Fusion gene:SPAG1-STK3 (FusionGDB2 ID:85431)

Fusion Gene Summary for SPAG1-STK3

check button Fusion gene summary
Fusion gene informationFusion gene name: SPAG1-STK3
Fusion gene ID: 85431
HgeneTgene
Gene symbol

SPAG1

STK3

Gene ID

6674

8428

Gene namesperm associated antigen 1serine/threonine kinase 24
SynonymsCILD28|CT140|HEL-S-268|HSD-3.8|SP75|TPISHEL-S-95|MST3|MST3B|STE20|STK3
Cytomap

8q22.2

13q32.2

Type of geneprotein-codingprotein-coding
Descriptionsperm-associated antigen 1TPR-containing protein involved in spermatogenesisepididymis secretory protein Li 268infertility-related sperm protein Spag-1tetratricopeptide repeat-containing proteinserine/threonine-protein kinase 24STE20-like kinase 3STE20-like kinase MST3epididymis secretory protein Li 95mammalian STE20-like protein kinase 3serine/threonine kinase 24 (STE20 homolog, yeast)sterile 20-like kinase 3
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST00000251809, ENST00000388798, 
ENST00000520508, ENST00000520643, 
ENST00000523302, 		ENST00000521768, 
ENST00000419617, ENST00000523601, 
Fusion gene scores* DoF score5 X 5 X 4=10010 X 8 X 7=560
# samples 610
** MAII scorelog2(6/100*10)=-0.736965594166206
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(10/560*10)=-2.48542682717024
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: SPAG1 [Title/Abstract] AND STK3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointSPAG1(101245765)-STK3(99719539), # samples:2
STK3(99718694)-SPAG1(101190043), # samples:1
Anticipated loss of major functional domain due to fusion event.STK3-SPAG1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
STK3-SPAG1 seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneSTK3

GO:0006468

protein phosphorylation

19604147

TgeneSTK3

GO:0042542

response to hydrogen peroxide

22291017

TgeneSTK3

GO:0046777

protein autophosphorylation

17046825|17657516


check buttonFusion gene breakpoints across SPAG1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across STK3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4LUSCTCGA-68-8250-01ASPAG1chr8

101245765

-STK3chr8

99719539

-
ChimerDB4LUSCTCGA-68-8250-01ASPAG1chr8

101245765

+STK3chr8

99719539

-


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Fusion Gene ORF analysis for SPAG1-STK3

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
5CDS-intronENST00000251809ENST00000521768SPAG1chr8

101245765

+STK3chr8

99719539

-
5CDS-intronENST00000388798ENST00000521768SPAG1chr8

101245765

+STK3chr8

99719539

-
In-frameENST00000251809ENST00000419617SPAG1chr8

101245765

+STK3chr8

99719539

-
In-frameENST00000251809ENST00000523601SPAG1chr8

101245765

+STK3chr8

99719539

-
In-frameENST00000388798ENST00000419617SPAG1chr8

101245765

+STK3chr8

99719539

-
In-frameENST00000388798ENST00000523601SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-3CDSENST00000520508ENST00000419617SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-3CDSENST00000520508ENST00000523601SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-3CDSENST00000520643ENST00000419617SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-3CDSENST00000520643ENST00000523601SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-3CDSENST00000523302ENST00000419617SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-3CDSENST00000523302ENST00000523601SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-intronENST00000520508ENST00000521768SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-intronENST00000520643ENST00000521768SPAG1chr8

101245765

+STK3chr8

99719539

-
intron-intronENST00000523302ENST00000521768SPAG1chr8

101245765

+STK3chr8

99719539

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000251809SPAG1chr8101245765+ENST00000419617STK3chr899719539-452922017433251083
ENST00000251809SPAG1chr8101245765+ENST00000523601STK3chr899719539-447322017433251083
ENST00000388798SPAG1chr8101245765+ENST00000419617STK3chr899719539-4634230619134301079
ENST00000388798SPAG1chr8101245765+ENST00000523601STK3chr899719539-4578230619134301079

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000251809ENST00000419617SPAG1chr8101245765+STK3chr899719539-0.0002560180.999744
ENST00000251809ENST00000523601SPAG1chr8101245765+STK3chr899719539-0.0002877960.99971217
ENST00000388798ENST00000419617SPAG1chr8101245765+STK3chr899719539-0.0002619280.99973804
ENST00000388798ENST00000523601SPAG1chr8101245765+STK3chr899719539-0.0002946080.9997054

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Fusion Genomic Features for SPAG1-STK3


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.
genomic feature

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for SPAG1-STK3


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:101245765/chr8:99719539)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619422_428705927.0Compositional biasNote=Poly-Ala
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619422_428705927.0Compositional biasNote=Poly-Ala
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619209_242705927.0RepeatNote=TPR 1
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619244_275705927.0RepeatNote=TPR 2
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619276_309705927.0RepeatNote=TPR 3
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619445_478705927.0RepeatNote=TPR 4
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619487_520705927.0RepeatNote=TPR 5
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619522_554705927.0RepeatNote=TPR 6
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619623_656705927.0RepeatNote=TPR 7
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619657_690705927.0RepeatNote=TPR 8
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619209_242705927.0RepeatNote=TPR 1
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619244_275705927.0RepeatNote=TPR 2
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619276_309705927.0RepeatNote=TPR 3
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619445_478705927.0RepeatNote=TPR 4
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619487_520705927.0RepeatNote=TPR 5
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619522_554705927.0RepeatNote=TPR 6
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619623_656705927.0RepeatNote=TPR 7
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619657_690705927.0RepeatNote=TPR 8
TgeneSTK3chr8:101245765chr8:99719539ENST00000419617311287_328117492.0Coiled coilOntology_term=ECO:0000255
TgeneSTK3chr8:101245765chr8:99719539ENST00000419617311442_475117492.0Coiled coilOntology_term=ECO:0000255
TgeneSTK3chr8:101245765chr8:99719539ENST00000419617311308_314117492.0Compositional biasNote=Poly-Glu
TgeneSTK3chr8:101245765chr8:99719539ENST00000419617311370_375117492.0Compositional biasNote=Poly-Glu
TgeneSTK3chr8:101245765chr8:99719539ENST00000419617311437_484117492.0DomainSARAH

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619781_788705927.0Nucleotide bindingGTP
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619781_788705927.0Nucleotide bindingGTP
HgeneSPAG1chr8:101245765chr8:99719539ENST00000251809+1619692_724705927.0RepeatNote=TPR 9
HgeneSPAG1chr8:101245765chr8:99719539ENST00000388798+1619692_724705927.0RepeatNote=TPR 9
TgeneSTK3chr8:101245765chr8:99719539ENST0000041961731127_278117492.0DomainProtein kinase
TgeneSTK3chr8:101245765chr8:99719539ENST0000041961731133_41117492.0Nucleotide bindingATP


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Fusion Gene Sequence for SPAG1-STK3


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>85431_85431_1_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000251809_STK3_chr8_99719539_ENST00000419617_length(transcript)=4529nt_BP=2201nt
GACGAGACCCTCCCTCAGCACCTGCAGTGCAGCCAGAGCCGGTGCGGCGGCCGCGACTTCCCCGACGCGGGGCGCTGAGAAGCACTATGA
CCACCAAAGATTATCCATCATTGTGGGGCTTTGGAACAACAAAAACATTCAAAATTCCCATTGAACATCTAGATTTCAAATACATTGAAA
AATGTTCAGATGTTAAACATCTGGAAAAAATTCTTTGCGTGCTCAGATCTGGTGAGGAAGGATATTATCCTGAACTTACAGAATTTTGTG
AAAAGCATCTTCAAGCCTTGGCCCCTGAAAGCAGAGCTTTGAGGAAAGATAAACCAGCAGCAACAGCAGCCAGTTTTACAGCTGAAGAAT
GGGAAAAAATTGATGGTGATATAAAGAGTTGGGTATCAGAAATTAAAAAAGAAGAAGATAAAATGCACTTTCATGAAACTGAGACATTTC
CAGCAATGAAAGATAATTTGCCTCCAGTTCGTGGTTCAAACAGCTGTCTTCATGTAGGCAAGGAAAAATATTCTAAAAGACCAACTAAAA
AGAAAACTCCAAGGGATTACGCGGAATGGGATAAATTTGACGTGGAGAAGGAATGTTTAAAAATTGATGAAGATTACAAAGAAAAGACGG
TAATAGACAAGTCACACTTGTCTAAAATTGAGACAAGAATAGATACAGCAGGTCTAACTGAGAAAGAAAAGGATTTTCTTGCCACTCGTG
AAAAGGAGAAAGGAAATGAAGCTTTCAACTCAGGAGATTATGAAGAAGCAGTGATGTATTATACCAGGAGCATATCAGCGCTTCCCACTG
TAGTTGCCTATAACAATCGAGCTCAAGCAGAAATCAAATTACAGAACTGGAATAGTGCTTTTCAGGATTGTGAAAAGGTCTTGGAGTTAG
AACCTGGAAACGTAAAGGCTCTTCTGCGTCGTGCTACTACATATAAACATCAAAACAAGCTCCGGGAAGCTACAGAAGATTTGAGTAAAG
TACTAGATGTTGAGCCTGATAATGATTTGGCCAAGAAAACCTTGTCAGAGGTTGAAAGAGATCTGAAAAATTCTGAAGCTGCATCTGAGA
CTCAAACCAAAGGGAAAAGGATGGTTATTCAGGAAATAGAAAACTCCGAAGATGAAGAAGGAAAAAGCGGAAGAAAACATGAAGATGGCG
GTGGAGATAAGAAGCCCGCGGAGCCGGCGGGAGCCGCGCGCGCCGCCCAGCCGTGCGTCATGGGCAACATCCAGAAGAAGCTGACTGGCA
AAGCCGAAGGCGGCAAGCGGCCGGCAAGGGGCGCGCCGCAGCGGGGCCAGACCCCGGAGGCCGGCGCGGACAAGCGGAGCCCACGGCGGG
CCTCTGCGGCGGCGGCGGCGGGCGGCGGCGCCACCGGGCATCCGGGCGGCGGGCAGGGCGCGGAGAACCCTGCCGGCCTGAAGAGCCAGG
GCAACGAGCTGTTCCGAAGCGGGCAGTTCGCCGAGGCGGCCGGCAAGTACTCGGCGGCAATCGCGCTCCTGGAGCCAGCAGGAAGTGAAA
TTGCAGATGATCTAAGTATCTTATATTCAAATAGAGCAGCATGTTACCTAAAAGAAGGAAACTGCAGTGGCTGCATTCAAGATTGTAACA
GGGCTCTGGAACTTCATCCATTCTCTATGAAACCTCTTCTGAGGCGGGCGATGGCCTATGAAACTCTAGAGCAGTATGGGAAAGCTTATG
TGGATTATAAAACAGTGTTGCAGATAGACTGTGGACTCCAGCTAGCAAATGACAGTGTTAACAGGCTATCAAGAATTTTAATGGAGCTGG
ATGGACCAAATTGGCGGGAGAAGCTGTCACCTATTCCTGCTGTGCCTGCTTCTGTGCCACTGCAAGCTTGGCATCCGGCAAAAGAGATGA
TCTCAAAACAAGCAGGAGACTCCAGCAGCCATCGCCAGCAGGGCATCACAGATGAAAAAACATTTAAAGCCCTTAAGGAAGAAGGAAATC
AATGTGTAAATGACAAAAACTATAAAGACGCCCTCAGTAAATACAGCGAATGCTTAAAGATTAACAATAAGGAATGTGCCATATATACAA
ACAGAGCTCTCTGTTACTTGAAGCTGTGCCAGTTTGAAGAAGCAAAGCAGGACTGTGATCAGGCACTTCAGCTAGCTGATGGGAACGTGA
AAGCCTTCTATAGACGAGCTCTGGCTCATAAAGGACTCAAGTTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGAC
TAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGACATGCAAAATTGGCAG
ATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTGATTC
AAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAACCTCCTTATGCTGATA
TACATCCAATGAGGGCTATTTTTATGATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACCGATT
TTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCAAGAATGCCAAACCTG
TATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAAAGACATGAGGAACAGCAACGAGAATTGGAAGAGGAAGAAG
AAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGGCCACAAGCACGATGA
GTGAAGGGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAGTGAGGATGAGGAAG
AAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATAAGCAAGACTTCAAGA
ATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAG
ATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGATGGAACGGGAGATAG
AAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTGAGTCT
AATTTCCTCTCTGTTTTTAACTATTCTGGAGACCAAGAAACCACTAGGAATTGAAGGAATATTTGGATATTTTTAATCCTAAGATTTTGC
CCTACAATTAGGCAGAGGTCAAAAAGTGACAATGGTACATGCCCAGGTAAATTCCCAAAAGGCAGAATTGACAGTTGTATCTGCTGTGCA
TTCACTCTAAGATGAGGAGAACAAAAGAAGTGTATTCTCTTGTTCTGTCAGCTGCATACCAGTAATAAAACTGTTATGAAATGGATTTTC
AAGGTCTCTAAACCTTGAAAATCCAAAGCTATTGTTGCATTGTACAGCACTGAAGGGCTTTATGTTACAATATTCTTTATTCCTATCTAG
TATACTAGGCTATTTATTGTATCCCCTTAGGTAAACTTATTTATTTATGCTATTTTGCTTTGTTTCATTTTTTAAGGACAAGATCAGGAT
AGCTTTGGTGAAGGTAGGGTCATATTAATATGATGATAATGTGCAACCAATTTATACTTTCTGCAGGGAGCTATGGGGTACATTCCTTGA
TTTCCAGGATAGTTTTTCAAATAGGAAAGCAATAATGGCAGTAGTTCTCAAATGGGCTAGGCCTTTTTTATATTGAAGCAATAATTCCAT
TTTTACCCTTTGAAATTTTGTTTTTTTGATTTTTGATGTTTGGTACAAATAGAACTATATATATTTAGGTAAAATAGATCTATCGTGTTT
AAAACCAAAGAAATCAATGGAACCCTTGCACAAAAAAGTGTGATAAATATTTTTAAATAAAAACTTAATACAAATGTAATTTGTTAATAT
TGTTTCATGTTTTATGTGTAGATCTAATAGCTGAACTGATTCAAACTGTAATAAGCTCATCAATTTCATTTCTATGAAAATGTGCTCTGT
TGTCACAGGATGTTTCTGTTGATTTTATTCATTTCCTGGGAATTGGTAAACATCATGTTCCTGATGATAACCCAGTAGCAAAAACATTTG
TACTGAGTGGTACAAGCCTTGGGGACTGAAAAAAAAAAGATTAAAACCATTAAAAAGAAACTCATTTTTACGCTGAATGAACATTTATAT
GATTGCATTGGGACCAGTCATTTCCTAAGCTACATATGGCCATCTTGACAGTGTTTTTTCTTTTGTGTGTTTAATTATTATGTGTAAATC

>85431_85431_1_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000251809_STK3_chr8_99719539_ENST00000419617_length(amino acids)=1083AA_BP=709
MRSTMTTKDYPSLWGFGTTKTFKIPIEHLDFKYIEKCSDVKHLEKILCVLRSGEEGYYPELTEFCEKHLQALAPESRALRKDKPAATAAS
FTAEEWEKIDGDIKSWVSEIKKEEDKMHFHETETFPAMKDNLPPVRGSNSCLHVGKEKYSKRPTKKKTPRDYAEWDKFDVEKECLKIDED
YKEKTVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAEIKLQNWNSAFQDCE
KVLELEPGNVKALLRRATTYKHQNKLREATEDLSKVLDVEPDNDLAKKTLSEVERDLKNSEAASETQTKGKRMVIQEIENSEDEEGKSGR
KHEDGGGDKKPAEPAGAARAAQPCVMGNIQKKLTGKAEGGKRPARGAPQRGQTPEAGADKRSPRRASAAAAAGGGATGHPGGGQGAENPA
GLKSQGNELFRSGQFAEAAGKYSAAIALLEPAGSEIADDLSILYSNRAACYLKEGNCSGCIQDCNRALELHPFSMKPLLRRAMAYETLEQ
YGKAYVDYKTVLQIDCGLQLANDSVNRLSRILMELDGPNWREKLSPIPAVPASVPLQAWHPAKEMISKQAGDSSSHRQQGITDEKTFKAL
KEEGNQCVNDKNYKDALSKYSECLKINNKECAIYTNRALCYLKLCQFEEAKQDCDQALQLADGNVKAFYRRALAHKGLKLIEDEIATILK
STLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGK
PPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRE
LEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFD
KQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQ

--------------------------------------------------------------
>85431_85431_2_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000251809_STK3_chr8_99719539_ENST00000523601_length(transcript)=4473nt_BP=2201nt
GACGAGACCCTCCCTCAGCACCTGCAGTGCAGCCAGAGCCGGTGCGGCGGCCGCGACTTCCCCGACGCGGGGCGCTGAGAAGCACTATGA
CCACCAAAGATTATCCATCATTGTGGGGCTTTGGAACAACAAAAACATTCAAAATTCCCATTGAACATCTAGATTTCAAATACATTGAAA
AATGTTCAGATGTTAAACATCTGGAAAAAATTCTTTGCGTGCTCAGATCTGGTGAGGAAGGATATTATCCTGAACTTACAGAATTTTGTG
AAAAGCATCTTCAAGCCTTGGCCCCTGAAAGCAGAGCTTTGAGGAAAGATAAACCAGCAGCAACAGCAGCCAGTTTTACAGCTGAAGAAT
GGGAAAAAATTGATGGTGATATAAAGAGTTGGGTATCAGAAATTAAAAAAGAAGAAGATAAAATGCACTTTCATGAAACTGAGACATTTC
CAGCAATGAAAGATAATTTGCCTCCAGTTCGTGGTTCAAACAGCTGTCTTCATGTAGGCAAGGAAAAATATTCTAAAAGACCAACTAAAA
AGAAAACTCCAAGGGATTACGCGGAATGGGATAAATTTGACGTGGAGAAGGAATGTTTAAAAATTGATGAAGATTACAAAGAAAAGACGG
TAATAGACAAGTCACACTTGTCTAAAATTGAGACAAGAATAGATACAGCAGGTCTAACTGAGAAAGAAAAGGATTTTCTTGCCACTCGTG
AAAAGGAGAAAGGAAATGAAGCTTTCAACTCAGGAGATTATGAAGAAGCAGTGATGTATTATACCAGGAGCATATCAGCGCTTCCCACTG
TAGTTGCCTATAACAATCGAGCTCAAGCAGAAATCAAATTACAGAACTGGAATAGTGCTTTTCAGGATTGTGAAAAGGTCTTGGAGTTAG
AACCTGGAAACGTAAAGGCTCTTCTGCGTCGTGCTACTACATATAAACATCAAAACAAGCTCCGGGAAGCTACAGAAGATTTGAGTAAAG
TACTAGATGTTGAGCCTGATAATGATTTGGCCAAGAAAACCTTGTCAGAGGTTGAAAGAGATCTGAAAAATTCTGAAGCTGCATCTGAGA
CTCAAACCAAAGGGAAAAGGATGGTTATTCAGGAAATAGAAAACTCCGAAGATGAAGAAGGAAAAAGCGGAAGAAAACATGAAGATGGCG
GTGGAGATAAGAAGCCCGCGGAGCCGGCGGGAGCCGCGCGCGCCGCCCAGCCGTGCGTCATGGGCAACATCCAGAAGAAGCTGACTGGCA
AAGCCGAAGGCGGCAAGCGGCCGGCAAGGGGCGCGCCGCAGCGGGGCCAGACCCCGGAGGCCGGCGCGGACAAGCGGAGCCCACGGCGGG
CCTCTGCGGCGGCGGCGGCGGGCGGCGGCGCCACCGGGCATCCGGGCGGCGGGCAGGGCGCGGAGAACCCTGCCGGCCTGAAGAGCCAGG
GCAACGAGCTGTTCCGAAGCGGGCAGTTCGCCGAGGCGGCCGGCAAGTACTCGGCGGCAATCGCGCTCCTGGAGCCAGCAGGAAGTGAAA
TTGCAGATGATCTAAGTATCTTATATTCAAATAGAGCAGCATGTTACCTAAAAGAAGGAAACTGCAGTGGCTGCATTCAAGATTGTAACA
GGGCTCTGGAACTTCATCCATTCTCTATGAAACCTCTTCTGAGGCGGGCGATGGCCTATGAAACTCTAGAGCAGTATGGGAAAGCTTATG
TGGATTATAAAACAGTGTTGCAGATAGACTGTGGACTCCAGCTAGCAAATGACAGTGTTAACAGGCTATCAAGAATTTTAATGGAGCTGG
ATGGACCAAATTGGCGGGAGAAGCTGTCACCTATTCCTGCTGTGCCTGCTTCTGTGCCACTGCAAGCTTGGCATCCGGCAAAAGAGATGA
TCTCAAAACAAGCAGGAGACTCCAGCAGCCATCGCCAGCAGGGCATCACAGATGAAAAAACATTTAAAGCCCTTAAGGAAGAAGGAAATC
AATGTGTAAATGACAAAAACTATAAAGACGCCCTCAGTAAATACAGCGAATGCTTAAAGATTAACAATAAGGAATGTGCCATATATACAA
ACAGAGCTCTCTGTTACTTGAAGCTGTGCCAGTTTGAAGAAGCAAAGCAGGACTGTGATCAGGCACTTCAGCTAGCTGATGGGAACGTGA
AAGCCTTCTATAGACGAGCTCTGGCTCATAAAGGACTCAAGTTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGAC
TAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGACATGCAAAATTGGCAG
ATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTGATTC
AAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAACCTCCTTATGCTGATA
TACATCCAATGAGGGCTATTTTTATGATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACCGATT
TTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCAAGAATGCCAAACCTG
TATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAAAGACATGAGGAACAGCAACGAGAATTGGAAGAGGAAGAAG
AAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGGCCACAAGCACGATGA
GTGAAGGGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAGTGAGGATGAGGAAG
AAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATAAGCAAGACTTCAAGA
ATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAG
ATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGATGGAACGGGAGATAG
AAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTGAGTCT
AATTTCCTCTCTGTTTTTAACTATTCTGGAGACCAAGAAACCACTAGGAATTGAAGGAATATTTGGATATTTTTAATCCTAAGATTTTGC
CCTACAATTAGGCAGAGGTCAAAAAGTGACAATGGTACATGCCCAGGTAAATTCCCAAAAGGCAGAATTGACAGTTGTATCTGCTGTGCA
TTCACTCTAAGATGAGGAGAACAAAAGAAGTGTATTCTCTTGTTCTGTCAGCTGCATACCAGTAATAAAACTGTTATGAAATGGATTTTC
AAGGTCTCTAAACCTTGAAAATCCAAAGCTATTGTTGCATTGTACAGCACTGAAGGGCTTTATGTTACAATATTCTTTATTCCTATCTAG
TATACTAGGCTATTTATTGTATCCCCTTAGGTAAACTTATTTATTTATGCTATTTTGCTTTGTTTCATTTTTTAAGGACAAGATCAGGAT
AGCTTTGGTGAAGGTAGGGTCATATTAATATGATGATAATGTGCAACCAATTTATACTTTCTGCAGGGAGCTATGGGGTACATTCCTTGA
TTTCCAGGATAGTTTTTCAAATAGGAAAGCAATAATGGCAGTAGTTCTCAAATGGGCTAGGCCTTTTTTATATTGAAGCAATAATTCCAT
TTTTACCCTTTGAAATTTTGTTTTTTTGATTTTTGATGTTTGGTACAAATAGAACTATATATATTTAGGTAAAATAGATCTATCGTGTTT
AAAACCAAAGAAATCAATGGAACCCTTGCACAAAAAAGTGTGATAAATATTTTTAAATAAAAACTTAATACAAATGTAATTTGTTAATAT
TGTTTCATGTTTTATGTGTAGATCTAATAGCTGAACTGATTCAAACTGTAATAAGCTCATCAATTTCATTTCTATGAAAATGTGCTCTGT
TGTCACAGGATGTTTCTGTTGATTTTATTCATTTCCTGGGAATTGGTAAACATCATGTTCCTGATGATAACCCAGTAGCAAAAACATTTG
TACTGAGTGGTACAAGCCTTGGGGACTGAAAAAAAAAAGATTAAAACCATTAAAAAGAAACTCATTTTTACGCTGAATGAACATTTATAT

>85431_85431_2_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000251809_STK3_chr8_99719539_ENST00000523601_length(amino acids)=1083AA_BP=709
MRSTMTTKDYPSLWGFGTTKTFKIPIEHLDFKYIEKCSDVKHLEKILCVLRSGEEGYYPELTEFCEKHLQALAPESRALRKDKPAATAAS
FTAEEWEKIDGDIKSWVSEIKKEEDKMHFHETETFPAMKDNLPPVRGSNSCLHVGKEKYSKRPTKKKTPRDYAEWDKFDVEKECLKIDED
YKEKTVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAEIKLQNWNSAFQDCE
KVLELEPGNVKALLRRATTYKHQNKLREATEDLSKVLDVEPDNDLAKKTLSEVERDLKNSEAASETQTKGKRMVIQEIENSEDEEGKSGR
KHEDGGGDKKPAEPAGAARAAQPCVMGNIQKKLTGKAEGGKRPARGAPQRGQTPEAGADKRSPRRASAAAAAGGGATGHPGGGQGAENPA
GLKSQGNELFRSGQFAEAAGKYSAAIALLEPAGSEIADDLSILYSNRAACYLKEGNCSGCIQDCNRALELHPFSMKPLLRRAMAYETLEQ
YGKAYVDYKTVLQIDCGLQLANDSVNRLSRILMELDGPNWREKLSPIPAVPASVPLQAWHPAKEMISKQAGDSSSHRQQGITDEKTFKAL
KEEGNQCVNDKNYKDALSKYSECLKINNKECAIYTNRALCYLKLCQFEEAKQDCDQALQLADGNVKAFYRRALAHKGLKLIEDEIATILK
STLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGK
PPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRE
LEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFD
KQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQ

--------------------------------------------------------------
>85431_85431_3_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000388798_STK3_chr8_99719539_ENST00000419617_length(transcript)=4634nt_BP=2306nt
GGGGCTCCCCAGTCCCGGCAGGTCCTGGCGCCGCTGTACATAGAAACCGTTGCCACGGCGGGAGGATGCCGTTTCTTTTCGGTCTCCGAC
TGCAGGGGGCGGTCGGCCCTGTGGATGCAGGAAAGAGGGTGCCTCGGTGGGAGAGACCCAGCTTTTCGCGTTAGTAGGCCCGAGCAGTTT
GAACTGGAGCTATGACCACCAAAGATTATCCATCATTGTGGGGCTTTGGAACAACAAAAACATTCAAAATTCCCATTGAACATCTAGATT
TCAAATACATTGAAAAATGTTCAGATGTTAAACATCTGGAAAAAATTCTTTGCGTGCTCAGATCTGGTGAGGAAGGATATTATCCTGAAC
TTACAGAATTTTGTGAAAAGCATCTTCAAGCCTTGGCCCCTGAAAGCAGAGCTTTGAGGAAAGATAAACCAGCAGCAACAGCAGCCAGTT
TTACAGCTGAAGAATGGGAAAAAATTGATGGTGATATAAAGAGTTGGGTATCAGAAATTAAAAAAGAAGAAGATAAAATGCACTTTCATG
AAACTGAGACATTTCCAGCAATGAAAGATAATTTGCCTCCAGTTCGTGGTTCAAACAGCTGTCTTCATGTAGGCAAGGAAAAATATTCTA
AAAGACCAACTAAAAAGAAAACTCCAAGGGATTACGCGGAATGGGATAAATTTGACGTGGAGAAGGAATGTTTAAAAATTGATGAAGATT
ACAAAGAAAAGACGGTAATAGACAAGTCACACTTGTCTAAAATTGAGACAAGAATAGATACAGCAGGTCTAACTGAGAAAGAAAAGGATT
TTCTTGCCACTCGTGAAAAGGAGAAAGGAAATGAAGCTTTCAACTCAGGAGATTATGAAGAAGCAGTGATGTATTATACCAGGAGCATAT
CAGCGCTTCCCACTGTAGTTGCCTATAACAATCGAGCTCAAGCAGAAATCAAATTACAGAACTGGAATAGTGCTTTTCAGGATTGTGAAA
AGGTCTTGGAGTTAGAACCTGGAAACGTAAAGGCTCTTCTGCGTCGTGCTACTACATATAAACATCAAAACAAGCTCCGGGAAGCTACAG
AAGATTTGAGTAAAGTACTAGATGTTGAGCCTGATAATGATTTGGCCAAGAAAACCTTGTCAGAGGTTGAAAGAGATCTGAAAAATTCTG
AAGCTGCATCTGAGACTCAAACCAAAGGGAAAAGGATGGTTATTCAGGAAATAGAAAACTCCGAAGATGAAGAAGGAAAAAGCGGAAGAA
AACATGAAGATGGCGGTGGAGATAAGAAGCCCGCGGAGCCGGCGGGAGCCGCGCGCGCCGCCCAGCCGTGCGTCATGGGCAACATCCAGA
AGAAGCTGACTGGCAAAGCCGAAGGCGGCAAGCGGCCGGCAAGGGGCGCGCCGCAGCGGGGCCAGACCCCGGAGGCCGGCGCGGACAAGC
GGAGCCCACGGCGGGCCTCTGCGGCGGCGGCGGCGGGCGGCGGCGCCACCGGGCATCCGGGCGGCGGGCAGGGCGCGGAGAACCCTGCCG
GCCTGAAGAGCCAGGGCAACGAGCTGTTCCGAAGCGGGCAGTTCGCCGAGGCGGCCGGCAAGTACTCGGCGGCAATCGCGCTCCTGGAGC
CAGCAGGAAGTGAAATTGCAGATGATCTAAGTATCTTATATTCAAATAGAGCAGCATGTTACCTAAAAGAAGGAAACTGCAGTGGCTGCA
TTCAAGATTGTAACAGGGCTCTGGAACTTCATCCATTCTCTATGAAACCTCTTCTGAGGCGGGCGATGGCCTATGAAACTCTAGAGCAGT
ATGGGAAAGCTTATGTGGATTATAAAACAGTGTTGCAGATAGACTGTGGACTCCAGCTAGCAAATGACAGTGTTAACAGGCTATCAAGAA
TTTTAATGGAGCTGGATGGACCAAATTGGCGGGAGAAGCTGTCACCTATTCCTGCTGTGCCTGCTTCTGTGCCACTGCAAGCTTGGCATC
CGGCAAAAGAGATGATCTCAAAACAAGCAGGAGACTCCAGCAGCCATCGCCAGCAGGGCATCACAGATGAAAAAACATTTAAAGCCCTTA
AGGAAGAAGGAAATCAATGTGTAAATGACAAAAACTATAAAGACGCCCTCAGTAAATACAGCGAATGCTTAAAGATTAACAATAAGGAAT
GTGCCATATATACAAACAGAGCTCTCTGTTACTTGAAGCTGTGCCAGTTTGAAGAAGCAAAGCAGGACTGTGATCAGGCACTTCAGCTAG
CTGATGGGAACGTGAAAGCCTTCTATAGACGAGCTCTGGCTCATAAAGGACTCAAGTTAATAGAAGATGAAATTGCAACCATTCTTAAAT
CTACATTGAAAGGACTAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGAC
ATGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGG
CTCCTGAGGTGATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAAC
CTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATGATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCG
ATGATTTCACCGATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCA
AGAATGCCAAACCTGTATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAAAGACATGAGGAACAGCAACGAGAAT
TGGAAGAGGAAGAAGAAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGG
CCACAAGCACGATGAGTGAAGGGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACA
GTGAGGATGAGGAAGAAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATA
AGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACT
GGAAAGTTCCTCAAGATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGA
TGGAACGGGAGATAGAAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGC
AAAACTTTTGAGTCTAATTTCCTCTCTGTTTTTAACTATTCTGGAGACCAAGAAACCACTAGGAATTGAAGGAATATTTGGATATTTTTA
ATCCTAAGATTTTGCCCTACAATTAGGCAGAGGTCAAAAAGTGACAATGGTACATGCCCAGGTAAATTCCCAAAAGGCAGAATTGACAGT
TGTATCTGCTGTGCATTCACTCTAAGATGAGGAGAACAAAAGAAGTGTATTCTCTTGTTCTGTCAGCTGCATACCAGTAATAAAACTGTT
ATGAAATGGATTTTCAAGGTCTCTAAACCTTGAAAATCCAAAGCTATTGTTGCATTGTACAGCACTGAAGGGCTTTATGTTACAATATTC
TTTATTCCTATCTAGTATACTAGGCTATTTATTGTATCCCCTTAGGTAAACTTATTTATTTATGCTATTTTGCTTTGTTTCATTTTTTAA
GGACAAGATCAGGATAGCTTTGGTGAAGGTAGGGTCATATTAATATGATGATAATGTGCAACCAATTTATACTTTCTGCAGGGAGCTATG
GGGTACATTCCTTGATTTCCAGGATAGTTTTTCAAATAGGAAAGCAATAATGGCAGTAGTTCTCAAATGGGCTAGGCCTTTTTTATATTG
AAGCAATAATTCCATTTTTACCCTTTGAAATTTTGTTTTTTTGATTTTTGATGTTTGGTACAAATAGAACTATATATATTTAGGTAAAAT
AGATCTATCGTGTTTAAAACCAAAGAAATCAATGGAACCCTTGCACAAAAAAGTGTGATAAATATTTTTAAATAAAAACTTAATACAAAT
GTAATTTGTTAATATTGTTTCATGTTTTATGTGTAGATCTAATAGCTGAACTGATTCAAACTGTAATAAGCTCATCAATTTCATTTCTAT
GAAAATGTGCTCTGTTGTCACAGGATGTTTCTGTTGATTTTATTCATTTCCTGGGAATTGGTAAACATCATGTTCCTGATGATAACCCAG
TAGCAAAAACATTTGTACTGAGTGGTACAAGCCTTGGGGACTGAAAAAAAAAAGATTAAAACCATTAAAAAGAAACTCATTTTTACGCTG
AATGAACATTTATATGATTGCATTGGGACCAGTCATTTCCTAAGCTACATATGGCCATCTTGACAGTGTTTTTTCTTTTGTGTGTTTAAT

>85431_85431_3_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000388798_STK3_chr8_99719539_ENST00000419617_length(amino acids)=1079AA_BP=705
MTTKDYPSLWGFGTTKTFKIPIEHLDFKYIEKCSDVKHLEKILCVLRSGEEGYYPELTEFCEKHLQALAPESRALRKDKPAATAASFTAE
EWEKIDGDIKSWVSEIKKEEDKMHFHETETFPAMKDNLPPVRGSNSCLHVGKEKYSKRPTKKKTPRDYAEWDKFDVEKECLKIDEDYKEK
TVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAEIKLQNWNSAFQDCEKVLE
LEPGNVKALLRRATTYKHQNKLREATEDLSKVLDVEPDNDLAKKTLSEVERDLKNSEAASETQTKGKRMVIQEIENSEDEEGKSGRKHED
GGGDKKPAEPAGAARAAQPCVMGNIQKKLTGKAEGGKRPARGAPQRGQTPEAGADKRSPRRASAAAAAGGGATGHPGGGQGAENPAGLKS
QGNELFRSGQFAEAAGKYSAAIALLEPAGSEIADDLSILYSNRAACYLKEGNCSGCIQDCNRALELHPFSMKPLLRRAMAYETLEQYGKA
YVDYKTVLQIDCGLQLANDSVNRLSRILMELDGPNWREKLSPIPAVPASVPLQAWHPAKEMISKQAGDSSSHRQQGITDEKTFKALKEEG
NQCVNDKNYKDALSKYSECLKINNKECAIYTNRALCYLKLCQFEEAKQDCDQALQLADGNVKAFYRRALAHKGLKLIEDEIATILKSTLK
GLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYA
DIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEE
EENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDF

--------------------------------------------------------------
>85431_85431_4_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000388798_STK3_chr8_99719539_ENST00000523601_length(transcript)=4578nt_BP=2306nt
GGGGCTCCCCAGTCCCGGCAGGTCCTGGCGCCGCTGTACATAGAAACCGTTGCCACGGCGGGAGGATGCCGTTTCTTTTCGGTCTCCGAC
TGCAGGGGGCGGTCGGCCCTGTGGATGCAGGAAAGAGGGTGCCTCGGTGGGAGAGACCCAGCTTTTCGCGTTAGTAGGCCCGAGCAGTTT
GAACTGGAGCTATGACCACCAAAGATTATCCATCATTGTGGGGCTTTGGAACAACAAAAACATTCAAAATTCCCATTGAACATCTAGATT
TCAAATACATTGAAAAATGTTCAGATGTTAAACATCTGGAAAAAATTCTTTGCGTGCTCAGATCTGGTGAGGAAGGATATTATCCTGAAC
TTACAGAATTTTGTGAAAAGCATCTTCAAGCCTTGGCCCCTGAAAGCAGAGCTTTGAGGAAAGATAAACCAGCAGCAACAGCAGCCAGTT
TTACAGCTGAAGAATGGGAAAAAATTGATGGTGATATAAAGAGTTGGGTATCAGAAATTAAAAAAGAAGAAGATAAAATGCACTTTCATG
AAACTGAGACATTTCCAGCAATGAAAGATAATTTGCCTCCAGTTCGTGGTTCAAACAGCTGTCTTCATGTAGGCAAGGAAAAATATTCTA
AAAGACCAACTAAAAAGAAAACTCCAAGGGATTACGCGGAATGGGATAAATTTGACGTGGAGAAGGAATGTTTAAAAATTGATGAAGATT
ACAAAGAAAAGACGGTAATAGACAAGTCACACTTGTCTAAAATTGAGACAAGAATAGATACAGCAGGTCTAACTGAGAAAGAAAAGGATT
TTCTTGCCACTCGTGAAAAGGAGAAAGGAAATGAAGCTTTCAACTCAGGAGATTATGAAGAAGCAGTGATGTATTATACCAGGAGCATAT
CAGCGCTTCCCACTGTAGTTGCCTATAACAATCGAGCTCAAGCAGAAATCAAATTACAGAACTGGAATAGTGCTTTTCAGGATTGTGAAA
AGGTCTTGGAGTTAGAACCTGGAAACGTAAAGGCTCTTCTGCGTCGTGCTACTACATATAAACATCAAAACAAGCTCCGGGAAGCTACAG
AAGATTTGAGTAAAGTACTAGATGTTGAGCCTGATAATGATTTGGCCAAGAAAACCTTGTCAGAGGTTGAAAGAGATCTGAAAAATTCTG
AAGCTGCATCTGAGACTCAAACCAAAGGGAAAAGGATGGTTATTCAGGAAATAGAAAACTCCGAAGATGAAGAAGGAAAAAGCGGAAGAA
AACATGAAGATGGCGGTGGAGATAAGAAGCCCGCGGAGCCGGCGGGAGCCGCGCGCGCCGCCCAGCCGTGCGTCATGGGCAACATCCAGA
AGAAGCTGACTGGCAAAGCCGAAGGCGGCAAGCGGCCGGCAAGGGGCGCGCCGCAGCGGGGCCAGACCCCGGAGGCCGGCGCGGACAAGC
GGAGCCCACGGCGGGCCTCTGCGGCGGCGGCGGCGGGCGGCGGCGCCACCGGGCATCCGGGCGGCGGGCAGGGCGCGGAGAACCCTGCCG
GCCTGAAGAGCCAGGGCAACGAGCTGTTCCGAAGCGGGCAGTTCGCCGAGGCGGCCGGCAAGTACTCGGCGGCAATCGCGCTCCTGGAGC
CAGCAGGAAGTGAAATTGCAGATGATCTAAGTATCTTATATTCAAATAGAGCAGCATGTTACCTAAAAGAAGGAAACTGCAGTGGCTGCA
TTCAAGATTGTAACAGGGCTCTGGAACTTCATCCATTCTCTATGAAACCTCTTCTGAGGCGGGCGATGGCCTATGAAACTCTAGAGCAGT
ATGGGAAAGCTTATGTGGATTATAAAACAGTGTTGCAGATAGACTGTGGACTCCAGCTAGCAAATGACAGTGTTAACAGGCTATCAAGAA
TTTTAATGGAGCTGGATGGACCAAATTGGCGGGAGAAGCTGTCACCTATTCCTGCTGTGCCTGCTTCTGTGCCACTGCAAGCTTGGCATC
CGGCAAAAGAGATGATCTCAAAACAAGCAGGAGACTCCAGCAGCCATCGCCAGCAGGGCATCACAGATGAAAAAACATTTAAAGCCCTTA
AGGAAGAAGGAAATCAATGTGTAAATGACAAAAACTATAAAGACGCCCTCAGTAAATACAGCGAATGCTTAAAGATTAACAATAAGGAAT
GTGCCATATATACAAACAGAGCTCTCTGTTACTTGAAGCTGTGCCAGTTTGAAGAAGCAAAGCAGGACTGTGATCAGGCACTTCAGCTAG
CTGATGGGAACGTGAAAGCCTTCTATAGACGAGCTCTGGCTCATAAAGGACTCAAGTTAATAGAAGATGAAATTGCAACCATTCTTAAAT
CTACATTGAAAGGACTAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGAC
ATGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGG
CTCCTGAGGTGATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAAC
CTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATGATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCG
ATGATTTCACCGATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCA
AGAATGCCAAACCTGTATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAAAGACATGAGGAACAGCAACGAGAAT
TGGAAGAGGAAGAAGAAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGG
CCACAAGCACGATGAGTGAAGGGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACA
GTGAGGATGAGGAAGAAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATA
AGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACT
GGAAAGTTCCTCAAGATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGA
TGGAACGGGAGATAGAAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGC
AAAACTTTTGAGTCTAATTTCCTCTCTGTTTTTAACTATTCTGGAGACCAAGAAACCACTAGGAATTGAAGGAATATTTGGATATTTTTA
ATCCTAAGATTTTGCCCTACAATTAGGCAGAGGTCAAAAAGTGACAATGGTACATGCCCAGGTAAATTCCCAAAAGGCAGAATTGACAGT
TGTATCTGCTGTGCATTCACTCTAAGATGAGGAGAACAAAAGAAGTGTATTCTCTTGTTCTGTCAGCTGCATACCAGTAATAAAACTGTT
ATGAAATGGATTTTCAAGGTCTCTAAACCTTGAAAATCCAAAGCTATTGTTGCATTGTACAGCACTGAAGGGCTTTATGTTACAATATTC
TTTATTCCTATCTAGTATACTAGGCTATTTATTGTATCCCCTTAGGTAAACTTATTTATTTATGCTATTTTGCTTTGTTTCATTTTTTAA
GGACAAGATCAGGATAGCTTTGGTGAAGGTAGGGTCATATTAATATGATGATAATGTGCAACCAATTTATACTTTCTGCAGGGAGCTATG
GGGTACATTCCTTGATTTCCAGGATAGTTTTTCAAATAGGAAAGCAATAATGGCAGTAGTTCTCAAATGGGCTAGGCCTTTTTTATATTG
AAGCAATAATTCCATTTTTACCCTTTGAAATTTTGTTTTTTTGATTTTTGATGTTTGGTACAAATAGAACTATATATATTTAGGTAAAAT
AGATCTATCGTGTTTAAAACCAAAGAAATCAATGGAACCCTTGCACAAAAAAGTGTGATAAATATTTTTAAATAAAAACTTAATACAAAT
GTAATTTGTTAATATTGTTTCATGTTTTATGTGTAGATCTAATAGCTGAACTGATTCAAACTGTAATAAGCTCATCAATTTCATTTCTAT
GAAAATGTGCTCTGTTGTCACAGGATGTTTCTGTTGATTTTATTCATTTCCTGGGAATTGGTAAACATCATGTTCCTGATGATAACCCAG
TAGCAAAAACATTTGTACTGAGTGGTACAAGCCTTGGGGACTGAAAAAAAAAAGATTAAAACCATTAAAAAGAAACTCATTTTTACGCTG

>85431_85431_4_SPAG1-STK3_SPAG1_chr8_101245765_ENST00000388798_STK3_chr8_99719539_ENST00000523601_length(amino acids)=1079AA_BP=705
MTTKDYPSLWGFGTTKTFKIPIEHLDFKYIEKCSDVKHLEKILCVLRSGEEGYYPELTEFCEKHLQALAPESRALRKDKPAATAASFTAE
EWEKIDGDIKSWVSEIKKEEDKMHFHETETFPAMKDNLPPVRGSNSCLHVGKEKYSKRPTKKKTPRDYAEWDKFDVEKECLKIDEDYKEK
TVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAEIKLQNWNSAFQDCEKVLE
LEPGNVKALLRRATTYKHQNKLREATEDLSKVLDVEPDNDLAKKTLSEVERDLKNSEAASETQTKGKRMVIQEIENSEDEEGKSGRKHED
GGGDKKPAEPAGAARAAQPCVMGNIQKKLTGKAEGGKRPARGAPQRGQTPEAGADKRSPRRASAAAAAGGGATGHPGGGQGAENPAGLKS
QGNELFRSGQFAEAAGKYSAAIALLEPAGSEIADDLSILYSNRAACYLKEGNCSGCIQDCNRALELHPFSMKPLLRRAMAYETLEQYGKA
YVDYKTVLQIDCGLQLANDSVNRLSRILMELDGPNWREKLSPIPAVPASVPLQAWHPAKEMISKQAGDSSSHRQQGITDEKTFKALKEEG
NQCVNDKNYKDALSKYSECLKINNKECAIYTNRALCYLKLCQFEEAKQDCDQALQLADGNVKAFYRRALAHKGLKLIEDEIATILKSTLK
GLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYA
DIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEE
EENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDF

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Fusion Gene PPI Analysis for SPAG1-STK3


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs for SPAG1-STK3


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

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Related Diseases for SPAG1-STK3


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource