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	Fusion Gene Summary
	Fusion Gene ORF analysis
	Fusion Genomic Features
	Fusion Protein Features
	Fusion Gene Sequence
	Fusion Gene PPI analysis
	Related Drugs
	Related Diseases

Fusion gene:USP37-ASF1B (FusionGDB2 ID:97434)

Fusion Gene Summary for USP37-ASF1B

Fusion gene summary

Fusion gene information	Fusion gene name: USP37-ASF1B
	Fusion gene ID: 97434
		Hgene	Tgene
	Gene symbol	USP37	ASF1B
	Gene ID	57695	55723
	Gene name	ubiquitin specific peptidase 37	anti-silencing function 1B histone chaperone
	Synonyms	-	CIA-II
	Cytomap	2q35	19p13.12
	Type of gene	protein-coding	protein-coding
	Description	ubiquitin carboxyl-terminal hydrolase 37deubiquitinating enzyme 37tmp_locus_50ubiquitin specific protease 37ubiquitin thioesterase 37ubiquitin thiolesterase 37ubiquitin-specific-processing protease 37	histone chaperone ASF1BASF1 anti-silencing function 1 homolog BCCG1-interacting factor A-IIanti-silencing function protein 1 homolog BhAsf1hAsf1bhCIA-II
	Modification date	20200313	20200313
	UniProtAcc	.	Q9NVP2
	Ensembl transtripts involved in fusion gene	ENST00000258399, ENST00000338465, ENST00000415516, ENST00000418019, ENST00000454775, ENST00000475553,	ENST00000474890, ENST00000263382, ENST00000592798,
Fusion gene scores	* DoF score	10 X 9 X 7=630	7 X 5 X 6=210
	# samples	11	8
	** MAII score	log2(11/630*10)=-2.51784830486262 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(8/210*10)=-1.39231742277876 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context	PubMed: USP37 [Title/Abstract] AND ASF1B [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint	USP37(219394679)-ASF1B(14231477), # samples:1
Anticipated loss of major functional domain due to fusion event.	USP37-ASF1B seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. USP37-ASF1B seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	USP37	GO:0000082	G1/S transition of mitotic cell cycle	21596315
Hgene	USP37	GO:0016579	protein deubiquitination	14715245
Hgene	USP37	GO:0035871	protein K11-linked deubiquitination	21596315
Hgene	USP37	GO:0071108	protein K48-linked deubiquitination	21596315
Tgene	ASF1B	GO:0006335	DNA replication-dependent nucleosome assembly	14718166
Tgene	ASF1B	GO:0006336	DNA replication-independent nucleosome assembly	14718166

Fusion gene breakpoints across USP37 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ASF1B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BRCA	TCGA-E9-A248-01A	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-

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Fusion Gene ORF analysis for USP37-ASF1B

Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

ORF	Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
5CDS-intron	ENST00000258399	ENST00000474890	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
5CDS-intron	ENST00000338465	ENST00000474890	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
5CDS-intron	ENST00000415516	ENST00000474890	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
5CDS-intron	ENST00000418019	ENST00000474890	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
5CDS-intron	ENST00000454775	ENST00000474890	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000258399	ENST00000263382	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000258399	ENST00000592798	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000338465	ENST00000263382	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000338465	ENST00000592798	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000415516	ENST00000263382	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000415516	ENST00000592798	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000418019	ENST00000263382	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000418019	ENST00000592798	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
Frame-shift	ENST00000454775	ENST00000592798	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
In-frame	ENST00000454775	ENST00000263382	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
intron-3CDS	ENST00000475553	ENST00000263382	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
intron-3CDS	ENST00000475553	ENST00000592798	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-
intron-intron	ENST00000475553	ENST00000474890	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-

ORFfinder result based on the fusion transcript sequence of in-frame fusion genes.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000454775

USP37

chr2

219394679

ENST00000263382

ASF1B

chr19

14231477

2437

1280

417

1316

299

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000454775	ENST00000263382	USP37	chr2	219394679	-	ASF1B	chr19	14231477	-	0.009549868	0.9904501

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Fusion Genomic Features for USP37-ASF1B

FusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

1-p

p (fusion gene breakpoint)

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

Distribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

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Fusion Protein Features for USP37-ASF1B

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:219394679/chr19:14231477)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	ASF1B Q9NVP2
FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.	FUNCTION: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. {ECO:0000269\|PubMed:11897662, ECO:0000269\|PubMed:12842904, ECO:0000269\|PubMed:14718166, ECO:0000269\|PubMed:15664198, ECO:0000269\|PubMed:16151251}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page

* Minus value of BPloci means that the break pointn is located before the CDS.

- In-frame and retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000258399

160_168

287

980.0

Motif

Note=D-box 3

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000258399

71_79

287

980.0

Motif

Note=D-box 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000258399

96_105

287

980.0

Motif

Note=D-box 2

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000415516

160_168

215

886.0

Motif

Note=D-box 3

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000415516

71_79

215

886.0

Motif

Note=D-box 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000415516

96_105

215

886.0

Motif

Note=D-box 2

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000418019

160_168

287

980.0

Motif

Note=D-box 3

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000418019

71_79

287

980.0

Motif

Note=D-box 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000418019

96_105

287

980.0

Motif

Note=D-box 2

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000454775

160_168

287

980.0

Motif

Note=D-box 3

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000454775

71_79

287

980.0

Motif

Note=D-box 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000454775

96_105

287

980.0

Motif

Note=D-box 2

- In-frame and not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000258399

341_951

287

980.0

Domain

Note=USP

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000258399

704_723

287

980.0

Domain

UIM 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000258399

806_825

287

980.0

Domain

UIM 2

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000258399

828_847

287

980.0

Domain

UIM 3

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000415516

341_951

215

886.0

Domain

Note=USP

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000415516

704_723

215

886.0

Domain

UIM 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000415516

806_825

215

886.0

Domain

UIM 2

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000415516

828_847

215

886.0

Domain

UIM 3

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000418019

341_951

287

980.0

Domain

Note=USP

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000418019

704_723

287

980.0

Domain

UIM 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000418019

806_825

287

980.0

Domain

UIM 2

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000418019

828_847

287

980.0

Domain

UIM 3

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000454775

341_951

287

980.0

Domain

Note=USP

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000454775

704_723

287

980.0

Domain

UIM 1

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000454775

806_825

287

980.0

Domain

UIM 2

Hgene

USP37

chr2:219394679

chr19:14231477

ENST00000454775

828_847

287

980.0

Domain

UIM 3

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Fusion Gene Sequence for USP37-ASF1B

For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>97434_97434_1_USP37-ASF1B_USP37_chr2_219394679_ENST00000454775_ASF1B_chr19_14231477_ENST00000263382_length(transcript)=2437nt_BP=1280nt
ATCAGCCGCTCTCCCTGACTAGGGGTAGCTAGCGGCCTCCCTCCTTTGGGTTCGGTATCCCTACTTCCATTACCCTCGGCTCCTCCCACT
CCTCGCGGGCTCCGTGCTTTCCGCGGGTCTGTCCGGGGGCTCCGGACCCTCGGCGCAGCTGAGTTGATGGCTTCCGGAGAACTGGCATAG
CTGCAGAATATGAGTAGTGTCCCAAGAAGAGTGCTTTGCCTTTGGCACAAGGATCAGAATAAAGGTGAATTGCTATTACATAGGGTTTTT
CGGTAAAAGTCACTGAAAATTATTTGCCATACAGTAACCAGAGACCTCCAACTAGGGGCCCCCAAACTGTATCCTGCCTGTAGAACCTGC
CAGGTAAAGGTATATTTTTGCTTTTTAATTTAGCCAGAAGCAATTTTTAAAGAAAATATGTCTCCTCTGAAGATACATGGTCCTATCAGA
ATTCGAAGTATGCAGACTGGGATTACAAAGTGGAAAGAAGGATCCTTTGAAATTGTAGAAAAAGAGAATAAAGTCAGCCTAGTAGTTCAC
TACAATACTGGAGGAATTCCAAGGATATTTCAGCTAAGTCATAACATTAAAAATGTGGTGCTTCGACCCAGTGGAGCGAAACAAAGCCGC
CTAATGTTAACTCTGCAAGATAACAGCTTCTTGTCTATTGACAAAGTACCAAGTAAGGATGCAGAGGAAATGAGGTTGTTTCTAGATGCA
GTCCATCAAAACAGACTTCCTGCAGCCATGAAACCGTCTCAGGGGTCTGGTAGTTTTGGAGCCATTCTGGGCAGCAGGACCTCACAGAAG
GAAACCAGCAGGCAGCTTTCTTACTCAGACAATCAGGCTTCTGCAAAAAGAGGAAGTTTGGAAACTAAAGATGATATTCCATTTCGAAAA
GTTCTTGGTAATCCGGGTAGAGGATCGATTAAGACTGTAGCAGGAAGTGGAATAGCTCGGACGATTCCTTCTTTGACATCTACTTCAACA
CCTCTTAGATCAGGGTTGCTAGAAAATCGTACTGAAAAGAGGAAAAGAATGATATCAACTGGCTCAGAATTGAATGAAGATTACCCTAAG
GAAAATGATTCATCATCGAACAACAAGGCCATGACAGATCCCTCCAGAAAGTATTTAACCAGCAGTAGAGAAAAGCAGCTGAGTTTGAAA
CAGTCAGAAGAGAATAGGACATCAGGGCTTTTACCTTTACAGTCATCATCCTTTTATGGTAGCAGAGCTGGATCCAAGGAACACTCTTCT
GGTGGCACTAACTTAGACAGCTCCAGCGGAACATCTTGGCCTCGAACCCCCGGGTGACCCGCTTCCATATCAACTGGGACAACAACATGG
ACAGGCTGGAGGCCATAGAGACCCAGGACCCCTCCCTGGGCTGCGGCCTCCCACTCAACTGCACTCCTATCAAGGGCTTGGGGCTCCCTG
GCTGCATCCCTGGCCTCCTCCCTGAGAACTCCATGGACTGCATCTAACTGCAGGAACCCAGAGTGTCCCAGCACGCCGGGAGGGGCAACC
AGGCCTCCCAGCGAGTCCTGCAGGGCCCATCTAGAGGACTTTGGGGGCCATCAGCTGCAATCCAGGTCTGTCAAACTCAGCCCCTAGGAA
AGAACAGGCCTTGGGTCTCCCCTAGTCCTGGCCAGAAGGATGATCTCGCTTTTCCTCTACAGGCCTATAAGAAGCAGGTACTTCAGTTCT
AAATTCTGACTTGTGTTCTTTTCGTCTTCATAAATTCTAACTAAGGCCACTGTGCCACTGTGCACCCTTGAGTACCATTGATCCAAAGCT
TTCCCACAGACCTCCCTGGCCCACCTAGAGGCTTTCTTGGTCAGTGCCTGTCAAGGCTCCAGTCCTGCTGAGCCAAAGGCTTTGTCATTC
CTTTCTCTTCCTGTACATCTGAGCAGACCCACTCCAGCTTTCTGGTGTCACAGGCGGGAATGTTAGTTAGTAGGTAGACTTAGATCCCAT
TTCTGTCCTGCTCCCAGGAAGATTCTTAGGTCCTCTTCAATCCAGCAGCCCCTCCCAGAGGTGTGATCAGCAGGATGCTGAGGAACCATG
TTGCCTTTCCTGTCAATCACAGCCACCTTCCTGTTATCTCCTAAATGGATCTGGCTTTTCCTGGAGGCTGCCATGGTTGGAAGATGGTAT
CAGAGGGCCTGCCTGGGCAGTCTGTCTCCGGGCCAGGGTCAGGGACCCTCTGCCTCTGGCAGCCTTAACCTGTCCTCTGCTAGGACCAGG
GTGATTTCAAGCCAGGGAAGCAACTGGGACCCTGAAAACTGTCCCTCCCCAGCCCGCTCCCCCTCTCTGTGCCCTGGTCCCCTTGCTGCC
ATGTGGATGCTGTTGTGATTGCTGTTTGTATATTATCAAAATGTTTTTATATTAAAAATGTTTGGTCTGAAAATTAAAAGCACTTCATTT

>97434_97434_1_USP37-ASF1B_USP37_chr2_219394679_ENST00000454775_ASF1B_chr19_14231477_ENST00000263382_length(amino acids)=299AA_BP=
MSPLKIHGPIRIRSMQTGITKWKEGSFEIVEKENKVSLVVHYNTGGIPRIFQLSHNIKNVVLRPSGAKQSRLMLTLQDNSFLSIDKVPSK
DAEEMRLFLDAVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYSDNQASAKRGSLETKDDIPFRKVLGNPGRGSIKTVAGSGIA
RTIPSLTSTSTPLRSGLLENRTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSLKQSEENRTSGLLPLQSSSFY

--------------------------------------------------------------

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Fusion Gene PPI Analysis for USP37-ASF1B

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)

Hgene

Hgene's interactors

Tgene

Tgene's interactors

- Retained PPIs in in-frame fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost PPIs in in-frame fusion.

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	ASF1B	chr2:219394679	chr19:14231477	ENST00000263382		2	4	1_155	134.0	203.0	CHAF1B
Tgene	ASF1B	chr2:219394679	chr19:14231477	ENST00000263382		2	4	1_156	134.0	203.0	histone H3

- Retained PPIs, but lost function due to frame-shift fusion.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs for USP37-ASF1B

Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)

Partner

Gene

UniProtAcc

DrugBank ID

Drug name

Drug activity

Drug type

Drug status

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Related Diseases for USP37-ASF1B

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source