FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact
Center for Computational Systems Medicine
leaf

Fusion Gene Summary

leaf

Fusion Gene ORF analysis

leaf

Fusion Genomic Features

leaf

Fusion Protein Features

leaf

Fusion Gene Sequence

leaf

Fusion Gene PPI analysis

leaf

Related Drugs

leaf

Related Diseases

Fusion gene:WNK1-EIF3G (FusionGDB2 ID:99326)

Fusion Gene Summary for WNK1-EIF3G

check button Fusion gene summary
Fusion gene informationFusion gene name: WNK1-EIF3G
Fusion gene ID: 99326
HgeneTgene
Gene symbol

WNK1

EIF3G

Gene ID

65125

8666

Gene nameWNK lysine deficient protein kinase 1eukaryotic translation initiation factor 3 subunit G
SynonymsHSAN2|HSN2|KDP|PPP1R167|PRKWNK1|PSK|p65EIF3-P42|EIF3S4|eIF3-delta|eIF3-p44
Cytomap

12p13.33

19p13.2

Type of geneprotein-codingprotein-coding
Descriptionserine/threonine-protein kinase WNK1WNK lysine deficient protein kinase 1 isoformerythrocyte 65 kDa proteinprostate-derived sterile 20-like kinaseprotein kinase with no lysine 1protein phosphatase 1, regulatory subunit 167serine/threonine-protein kieukaryotic translation initiation factor 3 subunit Geukaryotic translation initiation factor 3 RNA-binding subuniteukaryotic translation initiation factor 3 subunit p42eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa
Modification date2020032720200313
UniProtAcc.

O75821

Ensembl transtripts involved in fusion geneENST00000540360, ENST00000315939, 
ENST00000530271, ENST00000535572, 
ENST00000537687, ENST00000340908, 
ENST00000447667, ENST00000574564, 
ENST00000253108, ENST00000587168, 
Fusion gene scores* DoF score23 X 19 X 15=65555 X 5 X 3=75
# samples 375
** MAII scorelog2(37/6555*10)=-4.14699860459281
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(5/75*10)=-0.584962500721156
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context

PubMed: WNK1 [Title/Abstract] AND EIF3G [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpointWNK1(939326)-EIF3G(10230376), # samples:2
Anticipated loss of major functional domain due to fusion event.WNK1-EIF3G seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
WNK1-EIF3G seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
WNK1-EIF3G seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF.
WNK1-EIF3G seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
WNK1-EIF3G seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneWNK1

GO:0006468

protein phosphorylation

10660600

HgeneWNK1

GO:0010923

negative regulation of phosphatase activity

19389623

HgeneWNK1

GO:0023016

signal transduction by trans-phosphorylation

16669787

HgeneWNK1

GO:0035556

intracellular signal transduction

10660600

TgeneEIF3G

GO:0006413

translational initiation

17581632

TgeneEIF3G

GO:0075525

viral translational termination-reinitiation

21347434


check buttonFusion gene breakpoints across WNK1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across EIF3G (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4OVTCGA-09-2044-01BWNK1chr12

939326

+EIF3Gchr19

10230376

-
ChimerDB4OVTCGA-09-2044-01BWNK1chr12

939326

+EIF3Gchr19

10230655

-


Top

Fusion Gene ORF analysis for WNK1-EIF3G

check button Open reading frame (ORF) analsis of fusion genes based on Ensembl gene isoform structure.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
ORFHenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrand
3UTR-3CDSENST00000540360ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
3UTR-5UTRENST00000540360ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
3UTR-intronENST00000540360ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
3UTR-intronENST00000540360ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-5UTRENST00000315939ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
5CDS-5UTRENST00000530271ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
5CDS-5UTRENST00000535572ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
5CDS-5UTRENST00000537687ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
5CDS-intronENST00000315939ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-intronENST00000315939ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-intronENST00000530271ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-intronENST00000530271ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-intronENST00000535572ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-intronENST00000535572ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-intronENST00000537687ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
5CDS-intronENST00000537687ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-
Frame-shiftENST00000315939ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
Frame-shiftENST00000530271ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
Frame-shiftENST00000535572ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
Frame-shiftENST00000537687ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
intron-3CDSENST00000340908ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
intron-3CDSENST00000447667ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
intron-3CDSENST00000574564ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230376

-
intron-5UTRENST00000340908ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
intron-5UTRENST00000447667ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
intron-5UTRENST00000574564ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230376

-
intron-intronENST00000340908ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
intron-intronENST00000340908ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-
intron-intronENST00000447667ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
intron-intronENST00000447667ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-
intron-intronENST00000574564ENST00000253108WNK1chr12

939326

+EIF3Gchr19

10230655

-
intron-intronENST00000574564ENST00000587168WNK1chr12

939326

+EIF3Gchr19

10230655

-

check buttonORFfinder result based on the fusion transcript sequence of in-frame fusion genes.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

Top

Fusion Genomic Features for WNK1-EIF3G


check buttonFusionAI prediction of the potential fusion gene breakpoint based on the pre-mature RNA sequence context (+/- 5kb of individual partner genes, total 20kb length sequence). FusionAI is a fusion gene breakpoint classifier based on convolutional neural network by comparing the fusion positive and negative sequence context of ~ 20K fusion gene data. From here, we can have the relative potentency of the 20K genomic sequence how individual sequnce will be likely used as the gene fusion breakpoints.
HgeneHchrHbpHstrandTgeneTchrTbpTstrand1-pp (fusion gene breakpoint)

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions. We integrated a total of 44 different types of human genomic feature loci information across five big categories including virus integration sites, repeats, structural variants, chromatin states, and gene expression regulation. More details are in help page.

check buttonDistribution of 44 human genomic features loci across 20kb length fusion breakpoint regions that are ovelapped with the top 1% feature importance score regions. More details are in help page.

Top

Fusion Protein Features for WNK1-EIF3G


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:939326/:10230376)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.EIF3G

O75821

FUNCTION: Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). {ECO:0000250}.FUNCTION: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). This subunit can bind 18S rRNA. {ECO:0000255|HAMAP-Rule:MF_03006, ECO:0000269|PubMed:17581632, ECO:0000269|PubMed:25849773, ECO:0000269|PubMed:27462815}.; FUNCTION: (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426). {ECO:0000269|PubMed:18056426}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page


* Minus value of BPloci means that the break pointn is located before the CDS.
- In-frame and retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- In-frame and not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


Top

Fusion Gene Sequence for WNK1-EIF3G


check button For in-frame fusion transcripts, we provide the fusion transcript sequences and fusion amino acid sequences. To have fusion amino acid sequence, we ran ORFfinder and chose the longest ORF among the all predicted ones.

Top

Fusion Gene PPI Analysis for WNK1-EIF3G


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type (BIOGRID-3.4.160)
HgeneHgene's interactorsTgeneTgene's interactors


check button - Retained PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost PPIs in in-frame fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


check button - Retained PPIs, but lost function due to frame-shift fusion.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs for WNK1-EIF3G


check button Drugs targeting genes involved in this fusion gene.
(DrugBank Version 5.1.8 2021-05-08)
PartnerGeneUniProtAccDrugBank IDDrug nameDrug activityDrug typeDrug status

Top

Related Diseases for WNK1-EIF3G


check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource