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Fusion Protein:ZMYND8-TFE3 |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: ZMYND8-TFE3 | FusionPDB ID: 101420 | FusionGDB2.0 ID: 101420 | Hgene | Tgene | Gene symbol | ZMYND8 | TFE3 | Gene ID | 23613 | 7030 |
| Gene name | zinc finger MYND-type containing 8 | transcription factor binding to IGHM enhancer 3 | |
| Synonyms | PRKCBP1|PRO2893|RACK7 | RCCP2|RCCX1|TFEA|bHLHe33 | |
| Cytomap | 20q13.12 | Xp11.23 | |
| Type of gene | protein-coding | protein-coding | |
| Description | protein kinase C-binding protein 1CTCL tumor antigen se14-3cutaneous T-cell lymphoma-associated antigen se14-3predicted protein of HQ2893zinc finger MYND domain-containing protein 8 | transcription factor E3class E basic helix-loop-helix protein 33transcription factor E family, member Atranscription factor for IgH enhancertranscription factor for immunoglobulin heavy-chain enhancer 3 | |
| Modification date | 20200313 | 20200327 | |
| UniProtAcc | . | P19532 Main function of 5'-partner protein: FUNCTION: Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response (PubMed:2338243, PubMed:29146937, PubMed:30733432, PubMed:31672913). Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation (PubMed:31672913). Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in nuclear localization and transcription factor activity (PubMed:31672913). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer (PubMed:2338243). It also binds very well to a USF/MLTF site (PubMed:2338243). May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937). Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue (By similarity). Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB; mTOR-dependent nuclear exclusion promotes exit from pluripotency (By similarity). Required to maintain the naive pluripotent state of hematopoietic stem cell; mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency (PubMed:30733432). {ECO:0000250|UniProtKB:Q64092, ECO:0000269|PubMed:2338243, ECO:0000269|PubMed:29146937, ECO:0000269|PubMed:30733432, ECO:0000269|PubMed:31672913}. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000262975, ENST00000311275, ENST00000352431, ENST00000355972, ENST00000360911, ENST00000372023, ENST00000396281, ENST00000446994, ENST00000458360, ENST00000461685, ENST00000471951, ENST00000536340, ENST00000540497, ENST00000468376, | ENST00000487451, ENST00000315869, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 25 X 23 X 10=5750 | 14 X 15 X 6=1260 |
| # samples | 34 | 15 | |
| ** MAII score | log2(34/5750*10)=-4.0799553045814 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(15/1260*10)=-3.0703893278914 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: ZMYND8 [Title/Abstract] AND TFE3 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: ZMYND8 [Title/Abstract] AND TFE3 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | ZMYND8(45849965)-TFE3(48896935), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | ZMYND8-TFE3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. ZMYND8-TFE3 seems lost the major protein functional domain in Tgene partner, which is a transcription factor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr20:45849965/chrX:48896935) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across ZMYND8 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across TFE3 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000262975 | ZMYND8 | chr20 | 45849965 | - | ENST00000315869 | TFE3 | chrX | 48896935 | - | 6428 | 3503 | 239 | 3667 | 1142 |
| ENST00000446994 | ZMYND8 | chr20 | 45849965 | - | ENST00000315869 | TFE3 | chrX | 48896935 | - | 6044 | 3119 | 44 | 3283 | 1079 |
| ENST00000355972 | ZMYND8 | chr20 | 45849965 | - | ENST00000315869 | TFE3 | chrX | 48896935 | - | 6442 | 3517 | 115 | 3681 | 1188 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000262975 | ENST00000315869 | ZMYND8 | chr20 | 45849965 | - | TFE3 | chrX | 48896935 | - | 0.003532143 | 0.9964678 |
| ENST00000446994 | ENST00000315869 | ZMYND8 | chr20 | 45849965 | - | TFE3 | chrX | 48896935 | - | 0.002977521 | 0.99702245 |
| ENST00000355972 | ENST00000315869 | ZMYND8 | chr20 | 45849965 | - | TFE3 | chrX | 48896935 | - | 0.004038085 | 0.99596196 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for ZMYND8-TFE3 |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
| ZMYND8 | chr20 | 45849965 | TFE3 | chrX | 48896935 | 3119 | 1025 | KETSAEKSKESGSPPNITAGHTSHPS |
| ZMYND8 | chr20 | 45849965 | TFE3 | chrX | 48896935 | 3503 | 1088 | KETSAEKSKESGSPPNITAGHTSHPS |
| ZMYND8 | chr20 | 45849965 | TFE3 | chrX | 48896935 | 3517 | 1134 | KETSAEKSKESGSPPNITAGHTSHPS |
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Potential FusionNeoAntigen Information of ZMYND8-TFE3 in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| ZMYND8-TFE3_45849965_48896935.msa |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B13:01 | KESGSPPNI | 0.9895 | 0.7759 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B45:01 | KESGSPPNI | 0.965 | 0.6521 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-A30:08 | KSKESGSPP | 0.9648 | 0.5942 | 6 | 15 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B44:03 | KESGSPPNI | 0.9559 | 0.9158 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B41:01 | KESGSPPNI | 0.3045 | 0.7193 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B39:13 | KESGSPPNI | 0.2293 | 0.6884 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B50:01 | KESGSPPNI | 0.1943 | 0.5974 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B52:01 | KESGSPPNI | 0.006 | 0.8701 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B45:01 | AEKSKESGSP | 0.891 | 0.7059 | 4 | 14 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B45:01 | KESGSPPNITA | 0.9997 | 0.8235 | 8 | 19 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B50:02 | KESGSPPNITA | 0.9994 | 0.5073 | 8 | 19 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B45:01 | AEKSKESGSPP | 0.9979 | 0.6905 | 4 | 15 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B50:01 | KESGSPPNITA | 0.9973 | 0.6977 | 8 | 19 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B41:01 | KESGSPPNITA | 0.9958 | 0.8096 | 8 | 19 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B40:06 | KESGSPPNI | 0.9996 | 0.539 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B39:08 | KESGSPPNI | 0.343 | 0.602 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B40:06 | KESGSPPNITA | 0.9998 | 0.585 | 8 | 19 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B40:04 | KESGSPPNI | 0.9982 | 0.6124 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B44:07 | KESGSPPNI | 0.9559 | 0.9158 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B44:26 | KESGSPPNI | 0.9559 | 0.9158 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B44:13 | KESGSPPNI | 0.9559 | 0.9158 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B50:04 | KESGSPPNI | 0.1943 | 0.5974 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B50:05 | KESGSPPNI | 0.1943 | 0.5974 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B39:02 | KESGSPPNI | 0.1556 | 0.706 | 8 | 17 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B50:05 | KESGSPPNITA | 0.9973 | 0.6977 | 8 | 19 |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 | HLA-B50:04 | KESGSPPNITA | 0.9973 | 0.6977 | 8 | 19 |
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Potential FusionNeoAntigen Information of ZMYND8-TFE3 in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of ZMYND8-TFE3 |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
| File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
| 4601 | KSKESGSPPNITAG | ZMYND8 | TFE3 | chr20 | 45849965 | chrX | 48896935 | 3503 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ZMYND8-TFE3 |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
| HLA-B14:02 | 3BVN | 4601 | KSKESGSPPNITAG | -7.15543 | -7.26883 |
| HLA-B14:02 | 3BVN | 4601 | KSKESGSPPNITAG | -4.77435 | -5.80965 |
| HLA-B52:01 | 3W39 | 4601 | KSKESGSPPNITAG | -6.80875 | -6.92215 |
| HLA-B52:01 | 3W39 | 4601 | KSKESGSPPNITAG | -4.20386 | -5.23916 |
| HLA-A11:01 | 4UQ2 | 4601 | KSKESGSPPNITAG | -7.5194 | -8.5547 |
| HLA-A11:01 | 4UQ2 | 4601 | KSKESGSPPNITAG | -6.9601 | -7.0735 |
| HLA-A24:02 | 5HGA | 4601 | KSKESGSPPNITAG | -7.52403 | -7.63743 |
| HLA-A24:02 | 5HGA | 4601 | KSKESGSPPNITAG | -5.82433 | -6.85963 |
| HLA-B27:05 | 6PYJ | 4601 | KSKESGSPPNITAG | -3.28285 | -4.31815 |
| HLA-B44:05 | 3DX8 | 4601 | KSKESGSPPNITAG | -5.91172 | -6.94702 |
| HLA-B44:05 | 3DX8 | 4601 | KSKESGSPPNITAG | -4.24346 | -4.35686 |
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Vaccine Design for the FusionNeoAntigens of ZMYND8-TFE3 |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 4 | 14 | AEKSKESGSP | GCTGAGAAAAGCAAGGAGAGTGGCTCGCCT |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 4 | 15 | AEKSKESGSPP | GCTGAGAAAAGCAAGGAGAGTGGCTCGCCTCCC |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 6 | 15 | KSKESGSPP | AAAAGCAAGGAGAGTGGCTCGCCTCCC |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 8 | 17 | KESGSPPNI | AAGGAGAGTGGCTCGCCTCCCAATATC |
| ZMYND8-TFE3 | chr20 | 45849965 | chrX | 48896935 | 8 | 19 | KESGSPPNITA | AAGGAGAGTGGCTCGCCTCCCAATATCACTGCA |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of ZMYND8-TFE3 |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
| UCEC | ZMYND8-TFE3 | chr20 | 45849965 | ENST00000262975 | chrX | 48896935 | ENST00000315869 | TCGA-B5-A3FB-01A |
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Potential target of CAR-T therapy development for ZMYND8-TFE3 |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to ZMYND8-TFE3 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to ZMYND8-TFE3 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Tgene | TFE3 | C4518356 | MiT family translocation renal cell carcinoma | 2 | ORPHANET |
| Tgene | TFE3 | C0206657 | Alveolar Soft Part Sarcoma | 1 | ORPHANET |
| Tgene | TFE3 | C0206732 | Epithelioid hemangioendothelioma | 1 | ORPHANET |
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