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Fusion Protein:BRE-DTNB

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: BRE-DTNB
	FusionPDB ID: 10237
	FusionGDB2.0 ID: 10237
		Hgene	Tgene
	Gene symbol	BRE	DTNB
	Gene ID	9577	1838
	Gene name	BRISC and BRCA1 A complex member 2	dystrobrevin beta
	Synonyms	BRCC4\|BRCC45\|BRE	-
	Cytomap	2p23.2	2p23.3
	Type of gene	protein-coding	protein-coding
	Description	BRISC and BRCA1-A complex member 2BRCA1-A complex subunit BREBRCA1/BRCA2-containing complex subunit 45BRCA1/BRCA2-containing complex, subunit 4brain and reproductive organ-expressed (TNFRSF1A modulator)brain and reproductive organ-expressed protein	dystrobrevin betaDTN-Bbeta-dystrobrevin
	Modification date	20200313	20200327
	UniProtAcc	.	Q96EV8 Main function of 5'-partner protein: FUNCTION: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway. {ECO:0000269\|PubMed:15345706, ECO:0000269\|PubMed:16837549, ECO:0000269\|PubMed:17182842, ECO:0000269\|PubMed:17989303, ECO:0000269\|PubMed:19094965, ECO:0000269\|PubMed:20180862, ECO:0000269\|PubMed:20921223}.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000342045, ENST00000344773, ENST00000361704, ENST00000379624, ENST00000379632, ENST00000603461,	ENST00000288642, ENST00000404103, ENST00000405222, ENST00000406818, ENST00000407038, ENST00000407186, ENST00000407661, ENST00000472690, ENST00000545439, ENST00000496972,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	13 X 9 X 8=936	14 X 13 X 9=1638
	# samples	15	15
	** MAII score	log2(15/936*10)=-2.64154602908752 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(15/1638*10)=-3.44890095114513 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: BRE [Title/Abstract] AND DTNB [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: BRE [Title/Abstract] AND DTNB [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	BRE(28352247)-DTNB(25678363), # samples:2
Anticipated loss of major functional domain due to fusion event.	BRE-DTNB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BRE-DTNB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BRE-DTNB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. BRE-DTNB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	BRE	GO:0043066	negative regulation of apoptotic process	15465831

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:28352247/chr2:25678363)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across BRE (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across DTNB (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000344773	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25678363	-	1933	818	138	1547	469
ENST00000379624	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25678363	-	1912	797	117	1526	469
ENST00000342045	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25678363	-	1936	821	141	1550	469
ENST00000379632	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25678363	-	1935	820	140	1549	469
ENST00000361704	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25678363	-	1869	754	74	1483	469
ENST00000344773	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25674504	-	1843	818	138	1457	439
ENST00000344773	BRE	chr2	28352247	+	ENST00000405222	DTNB	chr2	25674504	-	1762	818	138	1421	427
ENST00000344773	BRE	chr2	28352247	+	ENST00000407661	DTNB	chr2	25674504	-	1819	818	138	1478	446
ENST00000344773	BRE	chr2	28352247	+	ENST00000406818	DTNB	chr2	25674504	-	1873	818	138	1532	464
ENST00000344773	BRE	chr2	28352247	+	ENST00000407038	DTNB	chr2	25674504	-	1783	818	138	1442	434
ENST00000344773	BRE	chr2	28352247	+	ENST00000404103	DTNB	chr2	25674504	-	1783	818	138	1442	434
ENST00000344773	BRE	chr2	28352247	+	ENST00000407186	DTNB	chr2	25674504	-	1837	818	138	1511	457
ENST00000344773	BRE	chr2	28352247	+	ENST00000288642	DTNB	chr2	25674504	-	1764	818	138	1442	434
ENST00000344773	BRE	chr2	28352247	+	ENST00000545439	DTNB	chr2	25674504	-	1579	818	138	1511	457
ENST00000379624	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25674504	-	1822	797	117	1436	439
ENST00000379624	BRE	chr2	28352247	+	ENST00000405222	DTNB	chr2	25674504	-	1741	797	117	1400	427
ENST00000379624	BRE	chr2	28352247	+	ENST00000407661	DTNB	chr2	25674504	-	1798	797	117	1457	446
ENST00000379624	BRE	chr2	28352247	+	ENST00000406818	DTNB	chr2	25674504	-	1852	797	117	1511	464
ENST00000379624	BRE	chr2	28352247	+	ENST00000407038	DTNB	chr2	25674504	-	1762	797	117	1421	434
ENST00000379624	BRE	chr2	28352247	+	ENST00000404103	DTNB	chr2	25674504	-	1762	797	117	1421	434
ENST00000379624	BRE	chr2	28352247	+	ENST00000407186	DTNB	chr2	25674504	-	1816	797	117	1490	457
ENST00000379624	BRE	chr2	28352247	+	ENST00000288642	DTNB	chr2	25674504	-	1743	797	117	1421	434
ENST00000379624	BRE	chr2	28352247	+	ENST00000545439	DTNB	chr2	25674504	-	1558	797	117	1490	457
ENST00000342045	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25674504	-	1846	821	141	1460	439
ENST00000342045	BRE	chr2	28352247	+	ENST00000405222	DTNB	chr2	25674504	-	1765	821	141	1424	427
ENST00000342045	BRE	chr2	28352247	+	ENST00000407661	DTNB	chr2	25674504	-	1822	821	141	1481	446
ENST00000342045	BRE	chr2	28352247	+	ENST00000406818	DTNB	chr2	25674504	-	1876	821	141	1535	464
ENST00000342045	BRE	chr2	28352247	+	ENST00000407038	DTNB	chr2	25674504	-	1786	821	141	1445	434
ENST00000342045	BRE	chr2	28352247	+	ENST00000404103	DTNB	chr2	25674504	-	1786	821	141	1445	434
ENST00000342045	BRE	chr2	28352247	+	ENST00000407186	DTNB	chr2	25674504	-	1840	821	141	1514	457
ENST00000342045	BRE	chr2	28352247	+	ENST00000288642	DTNB	chr2	25674504	-	1767	821	141	1445	434
ENST00000342045	BRE	chr2	28352247	+	ENST00000545439	DTNB	chr2	25674504	-	1582	821	141	1514	457
ENST00000379632	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25674504	-	1845	820	140	1459	439
ENST00000379632	BRE	chr2	28352247	+	ENST00000405222	DTNB	chr2	25674504	-	1764	820	140	1423	427
ENST00000379632	BRE	chr2	28352247	+	ENST00000407661	DTNB	chr2	25674504	-	1821	820	140	1480	446
ENST00000379632	BRE	chr2	28352247	+	ENST00000406818	DTNB	chr2	25674504	-	1875	820	140	1534	464
ENST00000379632	BRE	chr2	28352247	+	ENST00000407038	DTNB	chr2	25674504	-	1785	820	140	1444	434
ENST00000379632	BRE	chr2	28352247	+	ENST00000404103	DTNB	chr2	25674504	-	1785	820	140	1444	434
ENST00000379632	BRE	chr2	28352247	+	ENST00000407186	DTNB	chr2	25674504	-	1839	820	140	1513	457
ENST00000379632	BRE	chr2	28352247	+	ENST00000288642	DTNB	chr2	25674504	-	1766	820	140	1444	434
ENST00000379632	BRE	chr2	28352247	+	ENST00000545439	DTNB	chr2	25674504	-	1581	820	140	1513	457
ENST00000361704	BRE	chr2	28352247	+	ENST00000496972	DTNB	chr2	25674504	-	1779	754	74	1393	439
ENST00000361704	BRE	chr2	28352247	+	ENST00000405222	DTNB	chr2	25674504	-	1698	754	74	1357	427
ENST00000361704	BRE	chr2	28352247	+	ENST00000407661	DTNB	chr2	25674504	-	1755	754	74	1414	446
ENST00000361704	BRE	chr2	28352247	+	ENST00000406818	DTNB	chr2	25674504	-	1809	754	74	1468	464
ENST00000361704	BRE	chr2	28352247	+	ENST00000407038	DTNB	chr2	25674504	-	1719	754	74	1378	434
ENST00000361704	BRE	chr2	28352247	+	ENST00000404103	DTNB	chr2	25674504	-	1719	754	74	1378	434
ENST00000361704	BRE	chr2	28352247	+	ENST00000407186	DTNB	chr2	25674504	-	1773	754	74	1447	457
ENST00000361704	BRE	chr2	28352247	+	ENST00000288642	DTNB	chr2	25674504	-	1700	754	74	1378	434
ENST00000361704	BRE	chr2	28352247	+	ENST00000545439	DTNB	chr2	25674504	-	1515	754	74	1447	457

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000344773	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25678363	-	0.00395345	0.9960465
ENST00000379624	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25678363	-	0.003860896	0.9961391
ENST00000342045	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25678363	-	0.004046286	0.9959537
ENST00000379632	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25678363	-	0.004046378	0.9959537
ENST00000361704	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25678363	-	0.003597255	0.99640274
ENST00000344773	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003474481	0.99652547
ENST00000344773	ENST00000405222	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003513686	0.9964863
ENST00000344773	ENST00000407661	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003835157	0.9961649
ENST00000344773	ENST00000406818	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.004308172	0.99569184
ENST00000344773	ENST00000407038	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003748457	0.9962515
ENST00000344773	ENST00000404103	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003748457	0.9962515
ENST00000344773	ENST00000407186	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003715893	0.9962842
ENST00000344773	ENST00000288642	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003362978	0.996637
ENST00000344773	ENST00000545439	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003169602	0.9968304
ENST00000379624	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003282147	0.9967179
ENST00000379624	ENST00000405222	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003423682	0.99657625
ENST00000379624	ENST00000407661	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003713067	0.99628687
ENST00000379624	ENST00000406818	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.004269116	0.99573094
ENST00000379624	ENST00000407038	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003727594	0.99627244
ENST00000379624	ENST00000404103	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003727594	0.99627244
ENST00000379624	ENST00000407186	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003712373	0.9962876
ENST00000379624	ENST00000288642	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003370496	0.99662954
ENST00000379624	ENST00000545439	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003111214	0.9968888
ENST00000342045	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003234998	0.996765
ENST00000342045	ENST00000405222	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003383793	0.9966162
ENST00000342045	ENST00000407661	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.00366869	0.9963313
ENST00000342045	ENST00000406818	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.004376083	0.9956239
ENST00000342045	ENST00000407038	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003794775	0.9962053
ENST00000342045	ENST00000404103	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003794775	0.9962053
ENST00000342045	ENST00000407186	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003796686	0.99620336
ENST00000342045	ENST00000288642	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003497356	0.99650264
ENST00000342045	ENST00000545439	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.002957696	0.99704224
ENST00000379632	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.00324634	0.99675363
ENST00000379632	ENST00000405222	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003390666	0.99660933
ENST00000379632	ENST00000407661	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003677534	0.9963225
ENST00000379632	ENST00000406818	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.004395703	0.9956043
ENST00000379632	ENST00000407038	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003803126	0.99619687
ENST00000379632	ENST00000404103	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003803126	0.99619687
ENST00000379632	ENST00000407186	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003807765	0.9961922
ENST00000379632	ENST00000288642	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003506909	0.99649304
ENST00000379632	ENST00000545439	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.002971136	0.9970289
ENST00000361704	ENST00000496972	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.002994555	0.9970054
ENST00000361704	ENST00000405222	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003230904	0.9967691
ENST00000361704	ENST00000407661	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003281467	0.99671847
ENST00000361704	ENST00000406818	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003871867	0.9961281
ENST00000361704	ENST00000407038	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003506234	0.99649376
ENST00000361704	ENST00000404103	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003506234	0.99649376
ENST00000361704	ENST00000407186	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003339156	0.9966608
ENST00000361704	ENST00000288642	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.003044028	0.996956
ENST00000361704	ENST00000545439	BRE	chr2	28352247	+	DTNB	chr2	25674504	-	0.002733384	0.99726665

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for BRE-DTNB

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
BRE	chr2	28352247	DTNB	chr2	25674504	754	227	VYPKLYLSPRIEHVLDSPSRLDEEHR
BRE	chr2	28352247	DTNB	chr2	25674504	797	227	VYPKLYLSPRIEHVLDSPSRLDEEHR
BRE	chr2	28352247	DTNB	chr2	25674504	818	227	VYPKLYLSPRIEHVLDSPSRLDEEHR
BRE	chr2	28352247	DTNB	chr2	25674504	820	227	VYPKLYLSPRIEHVLDSPSRLDEEHR
BRE	chr2	28352247	DTNB	chr2	25674504	821	227	VYPKLYLSPRIEHVLDSPSRLDEEHR
BRE	chr2	28352247	DTNB	chr2	25678363	754	227	VYPKLYLSPRIEQLQYSQDIPSHLAD
BRE	chr2	28352247	DTNB	chr2	25678363	797	227	VYPKLYLSPRIEQLQYSQDIPSHLAD
BRE	chr2	28352247	DTNB	chr2	25678363	818	227	VYPKLYLSPRIEQLQYSQDIPSHLAD
BRE	chr2	28352247	DTNB	chr2	25678363	820	227	VYPKLYLSPRIEQLQYSQDIPSHLAD
BRE	chr2	28352247	DTNB	chr2	25678363	821	227	VYPKLYLSPRIEQLQYSQDIPSHLAD

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Potential FusionNeoAntigen Information of BRE-DTNB in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

BRE-DTNB_28352247_25674504.msa
BRE-DTNB_28352247_25678363.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:21	YLSPRIEHV	0.9992	0.5373	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A26:03	HVLDSPSRL	0.974	0.59	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A26:02	HVLDSPSRL	0.9698	0.5105	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:21	HVLDSPSRL	0.9291	0.855	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B13:01	YLSPRIEHV	0.2332	0.7597	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B13:01	HVLDSPSRL	0.1904	0.9785	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:01	EHVLDSPSRL	0.9987	0.9547	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:24	EHVLDSPSRL	0.9978	0.5796	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B38:02	EHVLDSPSRL	0.9977	0.9667	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B38:01	EHVLDSPSRL	0.9975	0.9577	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:24	YLSPRIEHVL	0.9841	0.5167	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:30	YLSPRIEHVL	0.9841	0.5167	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:67	YLSPRIEHVL	0.9841	0.5167	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:11	YLSPRIEHVL	0.9828	0.5515	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:35	YLSPRIEHVL	0.9456	0.537	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:10	EHVLDSPSRL	0.9438	0.6828	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:29	YLSPRIEHVL	0.9213	0.517	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:20	YLSPRIEHVL	0.8814	0.5212	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:37	EHVLDSPSRL	0.8641	0.6641	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:13	KLYLSPRIEHV	0.9988	0.5351	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:11	KLYLSPRIEHV	0.9959	0.6109	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:60	KLYLSPRIEHV	0.9958	0.5609	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:30	KLYLSPRIEHV	0.9955	0.5768	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:24	KLYLSPRIEHV	0.9955	0.5768	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:67	KLYLSPRIEHV	0.9955	0.5768	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:04	KLYLSPRIEHV	0.9948	0.5513	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:29	KLYLSPRIEHV	0.9766	0.5797	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:35	KLYLSPRIEHV	0.9606	0.594	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:20	KLYLSPRIEHV	0.9604	0.5779	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:10	SPRIEHVL	0.7131	0.7835	7	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:03	YLSPRIEHV	0.9997	0.9691	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:04	YLSPRIEHV	0.9997	0.9699	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:06	HVLDSPSRL	0.9995	0.9683	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:07	HVLDSPSRL	0.9995	0.9864	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:04	HVLDSPSRL	0.9993	0.9543	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C05:09	YLSPRIEHV	0.9991	0.755	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:08	HVLDSPSRL	0.999	0.9351	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:19	HVLDSPSRL	0.999	0.9862	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:08	LSPRIEHVL	0.9989	0.9062	6	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:10	YLSPRIEHV	0.9967	0.5786	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:06	YLSPRIEHV	0.996	0.7261	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:06	HVLDSPSRL	0.9957	0.9441	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:06	YLSPRIEHV	0.9927	0.7203	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:07	YLSPRIEHV	0.9922	0.6128	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:13	HVLDSPSRL	0.9901	0.9803	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:04	HVLDSPSRL	0.9901	0.9803	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:07	YLSPRIEHV	0.9851	0.9156	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:14	YLSPRIEHV	0.9825	0.6323	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:14	HVLDSPSRL	0.9814	0.9489	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:17	YLSPRIEHV	0.9426	0.7637	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:03	HVLDSPSRL	0.9315	0.9937	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:29	HVLDSPSRL	0.918	0.9759	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C02:06	HVLDSPSRL	0.9107	0.9775	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:03	HVLDSPSRL	0.9014	0.9965	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C02:06	YLSPRIEHV	0.8931	0.8587	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:17	LSPRIEHVL	0.8923	0.9003	6	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:14	HVLDSPSRL	0.8908	0.9909	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:04	HVLDSPSRL	0.8864	0.9962	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:12	HVLDSPSRL	0.8515	0.9717	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:13	YLSPRIEHV	0.8353	0.8863	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:04	YLSPRIEHV	0.8353	0.8863	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:16	YLSPRIEHV	0.8217	0.8613	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:30	LSPRIEHVL	0.806	0.9597	6	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:03	YLSPRIEHV	0.5595	0.8859	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:30	YLSPRIEHV	0.4473	0.8848	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:05	YLSPRIEHV	0.3102	0.8015	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:13	YLSPRIEHV	0.1538	0.7696	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:29	YLSPRIEHV	0.1291	0.8099	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:27	YLSPRIEHV	0.0942	0.8236	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:95	YLSPRIEHV	0.0501	0.5536	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:09	EHVLDSPSRL	0.9984	0.8159	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:12	EHVLDSPSRL	0.9981	0.9576	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:05	EHVLDSPSRL	0.9974	0.945	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:07	YLSPRIEHVL	0.9849	0.5343	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:01	YLSPRIEHVL	0.9841	0.5167	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:17	YLSPRIEHVL	0.9809	0.855	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:30	YLSPRIEHVL	0.9708	0.9289	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B14:03	EHVLDSPSRL	0.8547	0.9571	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:01	KLYLSPRIEHV	0.9955	0.5768	3	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B67:01	SPRIEHVL	0.6944	0.5569	7	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:05	HVLDSPSRL	0.9994	0.9459	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:09	HVLDSPSRL	0.9993	0.9543	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:06	YLSPRIEHV	0.9992	0.5373	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:02	HVLDSPSRL	0.9992	0.946	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C05:01	YLSPRIEHV	0.9991	0.755	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:03	HVLDSPSRL	0.999	0.9872	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:04	HVLDSPSRL	0.999	0.9872	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:03	YLSPRIEHV	0.999	0.664	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:17	LSPRIEHVL	0.9981	0.9734	6	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:17	YLSPRIEHV	0.9977	0.9644	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:02	YLSPRIEHV	0.9977	0.9644	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C18:01	YLSPRIEHV	0.9975	0.629	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:67	HVLDSPSRL	0.9969	0.9814	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:02	YLSPRIEHV	0.9964	0.6964	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:17	HVLDSPSRL	0.9958	0.9751	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C15:05	YLSPRIEHV	0.9957	0.7678	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:06	YLSPRIEHV	0.9951	0.9285	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:03	YLSPRIEHV	0.9951	0.9357	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:05	HVLDSPSRL	0.9945	0.9145	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:02	HVLDSPSRL	0.9939	0.9688	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:04	HVLDSPSRL	0.993	0.9454	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:01	YLSPRIEHV	0.9922	0.6128	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C16:02	YLSPRIEHV	0.991	0.9644	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:06	HVLDSPSRL	0.9902	0.9892	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:03	HVLDSPSRL	0.9866	0.9505	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:03	YLSPRIEHV	0.9855	0.8032	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:73	YLSPRIEHV	0.9782	0.729	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:06	HVLDSPSRL	0.9727	0.9958	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:06	LSPRIEHVL	0.9692	0.9866	6	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:03	HVLDSPSRL	0.9668	0.9762	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C04:04	YLSPRIEHV	0.966	0.7299	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A68:02	HVLDSPSRL	0.9592	0.7648	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B35:13	HVLDSPSRL	0.9566	0.9504	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C16:02	HVLDSPSRL	0.9528	0.997	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:30	YLSPRIEHV	0.952	0.5774	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A69:01	HVLDSPSRL	0.9442	0.8701	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:02	YLSPRIEHV	0.9396	0.7593	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:14	HVLDSPSRL	0.9336	0.7881	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:01	HVLDSPSRL	0.9315	0.9937	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:06	HVLDSPSRL	0.9291	0.855	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:02	YLSPRIEHV	0.9153	0.8707	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A69:01	YLSPRIEHV	0.911	0.5097	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:04	HVLDSPSRL	0.9014	0.9855	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:02	LSPRIEHVL	0.8947	0.8983	6	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:03	LSPRIEHVL	0.8866	0.8871	6	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C16:04	YLSPRIEHV	0.8849	0.9313	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:03	HVLDSPSRL	0.8741	0.9887	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C02:02	YLSPRIEHV	0.8709	0.8961	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C02:10	YLSPRIEHV	0.8709	0.8961	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:08	YLSPRIEHV	0.8611	0.9384	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C02:10	HVLDSPSRL	0.8594	0.9868	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C02:02	HVLDSPSRL	0.8594	0.9868	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C06:08	HVLDSPSRL	0.7822	0.9924	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C17:01	YLSPRIEHV	0.7739	0.6477	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C12:02	HVLDSPSRL	0.7606	0.9842	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:73	HVLDSPSRL	0.7258	0.9382	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B07:13	HVLDSPSRL	0.7126	0.9032	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C17:01	HVLDSPSRL	0.64	0.9615	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:30	HVLDSPSRL	0.598	0.9122	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C08:01	YLSPRIEHV	0.5595	0.8859	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:04	YLSPRIEHV	0.5226	0.7351	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C03:67	YLSPRIEHV	0.4948	0.8967	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B07:13	YLSPRIEHV	0.3399	0.5904	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B40:21	HVLDSPSRL	0.156	0.5152	12	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:01	YLSPRIEHV	0.0539	0.5365	5	14
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:31	EHVLDSPSRL	0.9986	0.9539	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B38:05	EHVLDSPSRL	0.9975	0.9577	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:03	YLSPRIEHVL	0.9933	0.861	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C01:02	YLSPRIEHVL	0.9859	0.8527	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:73	YLSPRIEHVL	0.9795	0.7427	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:14	YLSPRIEHVL	0.975	0.5091	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-C07:04	YLSPRIEHVL	0.9682	0.8118	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:09	EHVLDSPSRL	0.9546	0.5811	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B15:30	YLSPRIEHVL	0.9521	0.6056	5	15
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B39:11	EHVLDSPSRL	0.928	0.9366	11	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-B40:04	IEHVLDSPSRL	0.9996	0.6982	10	21
BRE-DTNB	chr2	28352247	chr2	25674504	821	HLA-A02:03	KLYLSPRIEHV	0.9994	0.5891	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:30	YLSPRIEQL	0.9983	0.6286	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:24	YLSPRIEQL	0.9983	0.6286	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:67	YLSPRIEQL	0.9983	0.6286	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:60	YLSPRIEQL	0.9982	0.5578	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:11	YLSPRIEQL	0.9982	0.6413	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:13	YLSPRIEQL	0.9978	0.6026	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:04	YLSPRIEQL	0.9977	0.5002	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:21	YLSPRIEQL	0.9974	0.7566	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:27	YLSPRIEQL	0.9971	0.5111	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:16	YLSPRIEQL	0.9961	0.5416	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:35	YLSPRIEQL	0.9908	0.6573	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:38	YLSPRIEQL	0.9888	0.5771	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:29	YLSPRIEQL	0.9773	0.6287	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:01	SPRIEQLQY	0.9697	0.8131	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:20	YLSPRIEQL	0.9562	0.6319	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B14:02	YLSPRIEQL	0.9483	0.7461	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B14:01	YLSPRIEQL	0.9483	0.7461	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:08	SPRIEQLQY	0.9416	0.7707	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:02	SPRIEQLQY	0.9376	0.8874	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A32:13	YLSPRIEQL	0.923	0.9375	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B39:01	YLSPRIEQL	0.666	0.9188	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B13:01	YLSPRIEQL	0.2686	0.9257	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:01	YLSPRIEQLQY	0.9999	0.8864	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:25	YLSPRIEQLQY	0.9984	0.8805	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:02	YLSPRIEQLQY	0.9969	0.8885	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:22	KLYLSPRIEQL	0.9945	0.5886	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:16	KLYLSPRIEQL	0.9932	0.6331	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:13	KLYLSPRIEQL	0.9922	0.6887	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:60	KLYLSPRIEQL	0.9917	0.6704	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:30	KLYLSPRIEQL	0.9914	0.7412	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:67	KLYLSPRIEQL	0.9914	0.7412	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:24	KLYLSPRIEQL	0.9914	0.7412	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:27	KLYLSPRIEQL	0.991	0.6505	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:11	KLYLSPRIEQL	0.991	0.7439	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:04	KLYLSPRIEQL	0.9907	0.6204	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:08	YLSPRIEQLQY	0.9758	0.7918	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:29	KLYLSPRIEQL	0.963	0.7415	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:20	KLYLSPRIEQL	0.9547	0.7416	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:01	YLSPRIEQLQY	0.9488	0.8534	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:35	KLYLSPRIEQL	0.9405	0.7643	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:17	LSPRIEQL	0.9996	0.9544	6	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:30	LSPRIEQL	0.9984	0.9704	6	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C12:12	YLSPRIEQL	0.9997	0.9244	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C06:03	YLSPRIEQL	0.9997	0.993	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C12:04	YLSPRIEQL	0.9997	0.9931	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C05:09	YLSPRIEQL	0.9994	0.8898	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C04:10	YLSPRIEQL	0.9991	0.8197	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C04:07	YLSPRIEQL	0.9988	0.8197	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:01	YLSPRIEQL	0.9983	0.6286	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:07	YLSPRIEQL	0.9983	0.6511	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:19	YLSPRIEQL	0.998	0.9881	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:07	YLSPRIEQL	0.9978	0.9776	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C15:04	YLSPRIEQL	0.9973	0.8777	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C15:06	YLSPRIEQL	0.9972	0.8938	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C04:06	YLSPRIEQL	0.9971	0.8856	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C12:16	YLSPRIEQL	0.9969	0.9601	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:08	YLSPRIEQL	0.9968	0.8996	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C08:15	YLSPRIEQL	0.9966	0.9524	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:17	YLSPRIEQL	0.9952	0.9146	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C04:14	YLSPRIEQL	0.9952	0.8676	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C08:04	YLSPRIEQL	0.991	0.9594	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C08:13	YLSPRIEQL	0.991	0.9594	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:04	YLSPRIEQL	0.9828	0.9402	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C02:06	YLSPRIEQL	0.9769	0.9616	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:31	SPRIEQLQY	0.9734	0.8013	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:13	YLSPRIEQL	0.9703	0.9081	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:29	YLSPRIEQL	0.9654	0.9391	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:14	YLSPRIEQL	0.9645	0.9789	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:05	YLSPRIEQL	0.9607	0.9626	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:10	YLSPRIEQL	0.95	0.9617	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:67	YLSPRIEQL	0.9451	0.9515	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:80	YLSPRIEQL	0.9451	0.9515	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C08:03	YLSPRIEQL	0.9446	0.9752	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B14:03	YLSPRIEQL	0.9412	0.7759	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:21	SPRIEQLQY	0.94	0.8636	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:30	YLSPRIEQL	0.9234	0.9497	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:27	YLSPRIEQL	0.9113	0.9582	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:19	YLSPRIEQL	0.904	0.7393	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B39:08	YLSPRIEQL	0.8925	0.7156	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:46	YLSPRIEQL	0.887	0.8987	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:95	YLSPRIEQL	0.8435	0.7158	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B39:09	YLSPRIEQL	0.7986	0.61	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B39:10	YLSPRIEQL	0.7152	0.9129	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B39:12	YLSPRIEQL	0.6586	0.9268	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B51:07	EQLQYSQDI	0.0748	0.6743	11	20
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:07	YLSPRIEQLQY	0.9999	0.6427	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:21	YLSPRIEQLQY	0.9963	0.8563	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:01	KLYLSPRIEQL	0.9914	0.7412	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:05	YLSPRIEQLQY	0.9682	0.8206	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:31	YLSPRIEQLQY	0.952	0.8362	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:03	LSPRIEQL	0.9996	0.9488	6	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:02	LSPRIEQL	0.9996	0.9529	6	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C12:03	YLSPRIEQL	0.9997	0.9792	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C04:03	YLSPRIEQL	0.9994	0.8442	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C05:01	YLSPRIEQL	0.9994	0.8898	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C06:17	YLSPRIEQL	0.9993	0.9924	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C06:02	YLSPRIEQL	0.9993	0.9924	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C06:06	YLSPRIEQL	0.999	0.9833	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:03	YLSPRIEQL	0.9989	0.61	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C18:01	YLSPRIEQL	0.9988	0.823	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C04:01	YLSPRIEQL	0.9988	0.8197	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C12:02	YLSPRIEQL	0.9986	0.9637	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:04	YLSPRIEQL	0.9982	0.9866	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:03	YLSPRIEQL	0.9982	0.9866	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C14:03	YLSPRIEQL	0.9978	0.9372	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C14:02	YLSPRIEQL	0.9978	0.9372	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:14	YLSPRIEQL	0.9975	0.5976	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:06	YLSPRIEQL	0.9974	0.7566	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C15:09	YLSPRIEQL	0.9973	0.8777	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C15:05	YLSPRIEQL	0.9969	0.8712	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:03	YLSPRIEQL	0.9968	0.9257	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C08:02	YLSPRIEQL	0.9966	0.9524	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C15:02	YLSPRIEQL	0.9964	0.8421	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:17	YLSPRIEQL	0.9963	0.9626	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:05	YLSPRIEQL	0.9962	0.9332	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C01:02	YLSPRIEQL	0.9962	0.9128	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C16:02	YLSPRIEQL	0.9962	0.9878	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C16:04	YLSPRIEQL	0.9961	0.9738	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:67	YLSPRIEQL	0.9959	0.977	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C04:04	YLSPRIEQL	0.9954	0.8843	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C16:01	YLSPRIEQL	0.9952	0.9747	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:02	YLSPRIEQL	0.9897	0.9729	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C03:06	YLSPRIEQL	0.9877	0.9868	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:04	YLSPRIEQL	0.9873	0.8799	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C02:10	YLSPRIEQL	0.9757	0.9761	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C02:02	YLSPRIEQL	0.9757	0.9761	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:77	SPRIEQLQY	0.9697	0.8131	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:23	SPRIEQLQY	0.9678	0.7602	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:24	YLSPRIEQL	0.9658	0.8965	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:73	YLSPRIEQL	0.9645	0.8494	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C06:08	YLSPRIEQL	0.9642	0.9878	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:20	SPRIEQLQY	0.9641	0.8665	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B08:12	YLSPRIEQL	0.956	0.5646	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:02	YLSPRIEQL	0.9451	0.9515	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C08:01	YLSPRIEQL	0.9446	0.9752	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:22	YLSPRIEQL	0.9438	0.698	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:17	YLSPRIEQL	0.9406	0.9583	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:11	SPRIEQLQY	0.9336	0.8379	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:24	SPRIEQLQY	0.9269	0.8941	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:30	YLSPRIEQL	0.9009	0.7389	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A32:01	YLSPRIEQL	0.8854	0.9432	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:30	SPRIEQLQY	0.8754	0.6315	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:17	SPRIEQLQY	0.8754	0.6315	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C17:01	YLSPRIEQL	0.8573	0.9131	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-C07:01	YLSPRIEQL	0.8496	0.6978	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B07:13	YLSPRIEQL	0.8314	0.817	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B39:11	YLSPRIEQL	0.8085	0.6872	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:13	YLSPRIEQL	0.7569	0.8939	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A69:01	YLSPRIEQL	0.75	0.8215	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B39:02	YLSPRIEQL	0.7015	0.9166	5	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:13	SPRIEQLQY	0.6609	0.5394	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:11	SPRIEQLQY	0.5448	0.8202	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:08	SPRIEQLQY	0.5368	0.8233	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:43	SPRIEQLQY	0.5036	0.8252	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B18:04	SPRIEQLQY	0.4243	0.9347	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B18:07	SPRIEQLQY	0.1319	0.9055	7	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B57:04	LSPRIEQLQY	0.9926	0.6671	6	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:11	LSPRIEQLQY	0.9919	0.8447	6	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:08	LSPRIEQLQY	0.9908	0.839	6	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:43	LSPRIEQLQY	0.9869	0.843	6	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:11	LSPRIEQLQY	0.9742	0.8645	6	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:17	LSPRIEQLQY	0.9422	0.7128	6	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:30	LSPRIEQLQY	0.9422	0.7128	6	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:27	YLSPRIEQLQY	0.9999	0.8911	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:43	YLSPRIEQLQY	0.9999	0.8375	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:11	YLSPRIEQLQY	0.9999	0.8348	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:34	YLSPRIEQLQY	0.9999	0.8864	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:08	YLSPRIEQLQY	0.9999	0.832	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:50	YLSPRIEQLQY	0.9999	0.8524	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:33	YLSPRIEQLQY	0.9999	0.8864	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:135	YLSPRIEQLQY	0.9999	0.8891	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:35	YLSPRIEQLQY	0.9999	0.8702	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:12	YLSPRIEQLQY	0.9999	0.8405	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:125	YLSPRIEQLQY	0.9999	0.8864	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:39	YLSPRIEQLQY	0.9984	0.7811	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-A02:03	KLYLSPRIEQL	0.9969	0.7115	3	14
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:11	YLSPRIEQLQY	0.9958	0.8554	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:20	YLSPRIEQLQY	0.9725	0.8813	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:28	YLSPRIEQLQY	0.9651	0.8784	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:17	YLSPRIEQLQY	0.9609	0.6941	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:30	YLSPRIEQLQY	0.9609	0.6941	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B15:13	YLSPRIEQLQY	0.9605	0.5385	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:23	YLSPRIEQLQY	0.9524	0.8448	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:77	YLSPRIEQLQY	0.9488	0.8534	5	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	HLA-B35:24	YLSPRIEQLQY	0.9215	0.9211	5	16

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Potential FusionNeoAntigen Information of BRE-DTNB in HLA II

BRE-DTNB_28352247_25674504.msa
BRE-DTNB_28352247_25678363.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0403	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0413	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0427	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0439	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0440	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0441	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0446	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0449	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0450	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0451	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0452	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0455	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0456	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0460	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0465	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0468	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0470	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0471	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0479	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0485	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25674504	821	DRB1-0488	SPRIEHVLDSPSRLD	7	22
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-0451	IEQLQYSQDIPSHLA	10	25
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-0478	IEQLQYSQDIPSHLA	10	25
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-1154	YPKLYLSPRIEQLQY	1	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-1154	VYPKLYLSPRIEQLQ	0	15
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-1189	YPKLYLSPRIEQLQY	1	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-1189	VYPKLYLSPRIEQLQ	0	15
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-1443	YPKLYLSPRIEQLQY	1	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB1-1445	YPKLYLSPRIEQLQY	1	16
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0101	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0104	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0105	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0108	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0109	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0111	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0112	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0113	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0114	EQLQYSQDIPSHLAD	11	26
BRE-DTNB	chr2	28352247	chr2	25678363	821	DRB3-0303	EQLQYSQDIPSHLAD	11	26

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Fusion breakpoint peptide structures of BRE-DTNB

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
5648	LSPRIEHVLDSPSR	BRE	DTNB	chr2	28352247	chr2	25674504	821

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
5649	LSPRIEQLQYSQDI	BRE	DTNB	chr2	28352247	chr2	25678363	821

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of BRE-DTNB

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	5648	LSPRIEHVLDSPSR	-7.02924	-7.14264
HLA-B14:02	3BVN	5648	LSPRIEHVLDSPSR	-3.38077	-4.41607
HLA-B52:01	3W39	5648	LSPRIEHVLDSPSR	-6.41355	-6.52695
HLA-B52:01	3W39	5648	LSPRIEHVLDSPSR	-4.44188	-5.47718
HLA-A24:02	5HGA	5648	LSPRIEHVLDSPSR	-7.76595	-8.80125
HLA-A24:02	5HGA	5648	LSPRIEHVLDSPSR	-7.30892	-7.42232
HLA-B44:05	3DX8	5648	LSPRIEHVLDSPSR	-5.65486	-5.76826
HLA-B44:05	3DX8	5648	LSPRIEHVLDSPSR	-2.95775	-3.99305
HLA-A03:01	2XPG	5649	LSPRIEQLQYSQDI	-1.81357	-2.84887
HLA-B14:02	3BVN	5649	LSPRIEQLQYSQDI	-6.73468	-6.84808
HLA-B14:02	3BVN	5649	LSPRIEQLQYSQDI	-3.28949	-4.32479
HLA-B52:01	3W39	5649	LSPRIEQLQYSQDI	-4.89713	-5.93243
HLA-B52:01	3W39	5649	LSPRIEQLQYSQDI	-4.21314	-4.32654
HLA-B18:01	4JQV	5649	LSPRIEQLQYSQDI	-4.94457	-5.05797
HLA-B18:01	4JQV	5649	LSPRIEQLQYSQDI	-3.33006	-4.36536
HLA-A11:01	4UQ2	5649	LSPRIEQLQYSQDI	-10.2413	-10.3547
HLA-A11:01	4UQ2	5649	LSPRIEQLQYSQDI	-8.98213	-10.0174
HLA-A24:02	5HGA	5649	LSPRIEQLQYSQDI	-5.24159	-5.35499
HLA-A24:02	5HGA	5649	LSPRIEQLQYSQDI	-3.84765	-4.88295
HLA-B27:05	6PYJ	5649	LSPRIEQLQYSQDI	-5.4025	-5.5159
HLA-B27:05	6PYJ	5649	LSPRIEQLQYSQDI	-1.28587	-2.32117
HLA-B27:03	6PZ5	5649	LSPRIEQLQYSQDI	10001	10000
HLA-B44:05	3DX8	5649	LSPRIEQLQYSQDI	-6.05269	-6.16609
HLA-B44:05	3DX8	5649	LSPRIEQLQYSQDI	-5.15192	-6.18722
HLA-A02:01	6TDR	5649	LSPRIEQLQYSQDI	-3.91416	-4.02756
HLA-A02:01	6TDR	5649	LSPRIEQLQYSQDI	-1.99837	-3.03367

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Vaccine Design for the FusionNeoAntigens of BRE-DTNB

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
BRE-DTNB	chr2	28352247	chr2	25674504	10	21	IEHVLDSPSRL	AATTGAGCATGTTCTGGACAGTCCTAGCCGACT
BRE-DTNB	chr2	28352247	chr2	25674504	11	21	EHVLDSPSRL	TGAGCATGTTCTGGACAGTCCTAGCCGACT
BRE-DTNB	chr2	28352247	chr2	25674504	12	21	HVLDSPSRL	GCATGTTCTGGACAGTCCTAGCCGACT
BRE-DTNB	chr2	28352247	chr2	25674504	3	14	KLYLSPRIEHV	CAAGCTGTACTTGTCACCTCGAATTGAGCATGT
BRE-DTNB	chr2	28352247	chr2	25674504	5	14	YLSPRIEHV	GTACTTGTCACCTCGAATTGAGCATGT
BRE-DTNB	chr2	28352247	chr2	25674504	5	15	YLSPRIEHVL	GTACTTGTCACCTCGAATTGAGCATGTTCT
BRE-DTNB	chr2	28352247	chr2	25674504	6	15	LSPRIEHVL	CTTGTCACCTCGAATTGAGCATGTTCT
BRE-DTNB	chr2	28352247	chr2	25674504	7	15	SPRIEHVL	GTCACCTCGAATTGAGCATGTTCT
BRE-DTNB	chr2	28352247	chr2	25678363	11	20	EQLQYSQDI	TGAGCAGTTACAGTATAGCCAGGATAT
BRE-DTNB	chr2	28352247	chr2	25678363	3	14	KLYLSPRIEQL	CAAGCTGTACTTGTCACCTCGAATTGAGCAGTT
BRE-DTNB	chr2	28352247	chr2	25678363	5	14	YLSPRIEQL	GTACTTGTCACCTCGAATTGAGCAGTT
BRE-DTNB	chr2	28352247	chr2	25678363	5	16	YLSPRIEQLQY	GTACTTGTCACCTCGAATTGAGCAGTTACAGTA
BRE-DTNB	chr2	28352247	chr2	25678363	6	14	LSPRIEQL	CTTGTCACCTCGAATTGAGCAGTT
BRE-DTNB	chr2	28352247	chr2	25678363	6	16	LSPRIEQLQY	CTTGTCACCTCGAATTGAGCAGTTACAGTA
BRE-DTNB	chr2	28352247	chr2	25678363	7	16	SPRIEQLQY	GTCACCTCGAATTGAGCAGTTACAGTA

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
BRE-DTNB	chr2	28352247	chr2	25674504	7	22	SPRIEHVLDSPSRLD	GTCACCTCGAATTGAGCATGTTCTGGACAGTCCTAGCCGACTGGA
BRE-DTNB	chr2	28352247	chr2	25678363	0	15	VYPKLYLSPRIEQLQ	GGTGTACCCCAAGCTGTACTTGTCACCTCGAATTGAGCAGTTACA
BRE-DTNB	chr2	28352247	chr2	25678363	1	16	YPKLYLSPRIEQLQY	GTACCCCAAGCTGTACTTGTCACCTCGAATTGAGCAGTTACAGTA
BRE-DTNB	chr2	28352247	chr2	25678363	10	25	IEQLQYSQDIPSHLA	AATTGAGCAGTTACAGTATAGCCAGGATATACCCAGTCACTTGGC
BRE-DTNB	chr2	28352247	chr2	25678363	11	26	EQLQYSQDIPSHLAD	TGAGCAGTTACAGTATAGCCAGGATATACCCAGTCACTTGGCCGA

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Information of the samples that have these potential fusion neoantigens of BRE-DTNB

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
BLCA	BRE-DTNB	chr2	28352247	ENST00000342045	chr2	25674504	ENST00000288642	TCGA-G2-A2EC-01A
BLCA	BRE-DTNB	chr2	28352247	ENST00000342045	chr2	25678363	ENST00000496972	TCGA-G2-A2EC-01A

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Potential target of CAR-T therapy development for BRE-DTNB

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to BRE-DTNB

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to BRE-DTNB

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)