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Fusion Protein:ZP3-C7orf73

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ZP3-C7orf73
	FusionPDB ID: 103187
	FusionGDB2.0 ID: 103187
		Hgene	Tgene
	Gene symbol	ZP3	C7orf73
	Gene ID	7784	647087
	Gene name	zona pellucida glycoprotein 3	short transmembrane mitochondrial protein 1
	Synonyms	OOMD3\|ZP3A\|ZP3B\|ZPC\|Zp-3	C7orf73\|Mm47\|PL-5283
	Cytomap	7q11.23	7q33
	Type of gene	protein-coding	protein-coding
	Description	zona pellucida sperm-binding protein 3ZP3A/ZP3Bsperm receptorzona pellucida glycoprotein 3Bzona pellucida glycoprotein ZP3zona pellucida protein C	short transmembrane mitochondrial protein 1uncharacterized protein C7orf73
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000336517, ENST00000394857, ENST00000416245, ENST00000467555,	ENST00000422968, ENST00000507606,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	2 X 2 X 2=8	8 X 7 X 4=224
	# samples	2	9
	** MAII score	log2(2/8*10)=1.32192809488736	log2(9/224*10)=-1.31550182572793 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: ZP3 [Title/Abstract] AND C7orf73 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: ZP3 [Title/Abstract] AND C7orf73 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ZP3(76063472)-C7orf73(135357500), # samples:1
Anticipated loss of major functional domain due to fusion event.	ZP3-C7orf73 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ZP3-C7orf73 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ZP3	GO:0002922	positive regulation of humoral immune response	19004505
Hgene	ZP3	GO:0007339	binding of sperm to zona pellucida	19246320\|19700799
Hgene	ZP3	GO:2000344	positive regulation of acrosome reaction	11751269\|18667750\|20394732
Hgene	ZP3	GO:2000360	negative regulation of binding of sperm to zona pellucida	18667750
Hgene	ZP3	GO:2000368	positive regulation of acrosomal vesicle exocytosis	19246320

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:76063472/chr7:135357500)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across ZP3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across C7orf73 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000336517

ZP3

chr7

76063472

ENST00000507606

C7orf73

chr7

135357500

2212

1024

1152

363

ENST00000394857

ZP3

chr7

76063472

ENST00000507606

C7orf73

chr7

135357500

2077

889

1017

319

ENST00000416245

ZP3

chr7

76063472

ENST00000507606

C7orf73

chr7

135357500

2584

1396

820

1524

234

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000336517	ENST00000507606	ZP3	chr7	76063472	+	C7orf73	chr7	135357500	+	0.001159759	0.9988403
ENST00000394857	ENST00000507606	ZP3	chr7	76063472	+	C7orf73	chr7	135357500	+	0.000539737	0.9994603
ENST00000416245	ENST00000507606	ZP3	chr7	76063472	+	C7orf73	chr7	135357500	+	0.000372657	0.99962735

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ZP3-C7orf73

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
ZP3	chr7	76063472	C7orf73	chr7	135357500	1024	321	VDVFHFANDSRNMLGFTLGNVVGMYL
ZP3	chr7	76063472	C7orf73	chr7	135357500	1396	192	VDVFHFANDSRNMLGFTLGNVVGMYL
ZP3	chr7	76063472	C7orf73	chr7	135357500	889	277	VDVFHFANDSRNMLGFTLGNVVGMYL

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Potential FusionNeoAntigen Information of ZP3-C7orf73 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

ZP3-C7orf73_76063472_135357500.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:04	SRNMLGFTL	0.9997	0.7523	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:02	SRNMLGFTL	0.9997	0.6555	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:05	SRNMLGFTL	0.9997	0.9218	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:24	SRNMLGFTL	0.9996	0.6864	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:01	SRNMLGFTL	0.9993	0.9625	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:06	SRNMLGFTL	0.9991	0.8106	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B38:02	SRNMLGFTL	0.9982	0.9904	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B38:01	SRNMLGFTL	0.9981	0.9903	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B14:01	SRNMLGFTL	0.9979	0.8222	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B14:02	SRNMLGFTL	0.9979	0.8222	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:03	FANDSRNML	0.979	0.9492	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:10	SRNMLGFTL	0.9603	0.6147	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:04	FANDSRNML	0.8975	0.969	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:02	FANDSRNML	0.8975	0.969	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:18	SRNMLGFTL	0.7688	0.7715	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:37	SRNMLGFTL	0.7591	0.6617	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:01	FHFANDSRNML	0.9999	0.883	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B38:02	FHFANDSRNML	0.9997	0.9252	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:06	FHFANDSRNML	0.9997	0.7643	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B38:01	FHFANDSRNML	0.9996	0.9197	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:10	FHFANDSRNML	0.9978	0.5627	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:37	FHFANDSRNML	0.9906	0.548	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:14	SRNMLGFTL	0.9997	0.8413	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:07	FANDSRNML	0.9997	0.9875	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:19	FANDSRNML	0.9997	0.9908	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:08	FANDSRNML	0.9997	0.9302	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C05:09	FANDSRNML	0.9995	0.9626	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:09	SRNMLGFTL	0.9994	0.8458	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C15:06	FANDSRNML	0.9992	0.9418	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C15:04	FANDSRNML	0.9992	0.8989	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:12	SRNMLGFTL	0.9992	0.9663	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:05	SRNMLGFTL	0.9992	0.9736	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:95	SRNMLGFTL	0.9992	0.8135	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C04:10	FANDSRNML	0.999	0.6411	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C08:15	FANDSRNML	0.9989	0.9805	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C04:07	FANDSRNML	0.9987	0.6649	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C01:17	FANDSRNML	0.9985	0.9393	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:27	SRNMLGFTL	0.9985	0.9702	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:05	SRNMLGFTL	0.9979	0.9521	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C12:12	FANDSRNML	0.9979	0.9216	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:29	SRNMLGFTL	0.9976	0.9609	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:03	FANDSRNML	0.9976	0.9953	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C12:04	FANDSRNML	0.9975	0.9958	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:13	SRNMLGFTL	0.9973	0.9612	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C04:06	FANDSRNML	0.9969	0.8643	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C08:04	FANDSRNML	0.9952	0.9711	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C08:13	FANDSRNML	0.9952	0.9711	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C12:16	FANDSRNML	0.9936	0.9795	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:03	SRNMLGFTL	0.9926	0.9368	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C01:30	FANDSRNML	0.9876	0.9565	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C04:14	FANDSRNML	0.9854	0.7011	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C02:06	FANDSRNML	0.9842	0.9771	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B14:03	FANDSRNML	0.9811	0.8403	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:46	SRNMLGFTL	0.9769	0.9391	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:14	FANDSRNML	0.9768	0.9823	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B73:01	SRNMLGFTL	0.9735	0.7191	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:05	FANDSRNML	0.9696	0.9709	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C08:03	FANDSRNML	0.9644	0.9887	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:13	FANDSRNML	0.9612	0.8821	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:19	SRNMLGFTL	0.9526	0.8708	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:67	SRNMLGFTL	0.9403	0.9671	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:80	SRNMLGFTL	0.9403	0.9671	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:29	FANDSRNML	0.9287	0.9599	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:27	FANDSRNML	0.9282	0.9643	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:12	FANDSRNML	0.8975	0.969	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:10	SRNMLGFTL	0.8915	0.9731	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:10	FANDSRNML	0.8879	0.9276	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:95	FANDSRNML	0.8791	0.6764	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B78:01	FANDSRNML	0.6125	0.5684	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B14:03	SRNMLGFTL	0.4932	0.8712	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C12:16	SRNMLGFTL	0.1765	0.9782	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:12	FHFANDSRNML	0.9998	0.8832	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:05	FHFANDSRNML	0.9996	0.871	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:14	FANDSRNMLGF	0.9991	0.9723	5	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:04	FANDSRNML	0.9998	0.9932	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:06	SRNMLGFTL	0.9998	0.7394	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:03	FANDSRNML	0.9998	0.9932	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:08	SRNMLGFTL	0.9997	0.819	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:09	SRNMLGFTL	0.9997	0.9118	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B27:10	SRNMLGFTL	0.9997	0.8943	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:67	FANDSRNML	0.9995	0.9868	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C05:01	FANDSRNML	0.9995	0.9626	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C04:03	FANDSRNML	0.9995	0.7284	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:05	FANDSRNML	0.9994	0.9532	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:17	FANDSRNML	0.9994	0.9778	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:02	SRNMLGFTL	0.9993	0.9728	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:31	SRNMLGFTL	0.9993	0.9637	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C15:09	FANDSRNML	0.9992	0.8989	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C18:01	FANDSRNML	0.9992	0.6847	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:01	SRNMLGFTL	0.9992	0.7998	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C15:05	FANDSRNML	0.999	0.946	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C08:02	FANDSRNML	0.9989	0.9805	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C01:03	FANDSRNML	0.9988	0.9068	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C04:01	FANDSRNML	0.9987	0.6649	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C15:02	FANDSRNML	0.9987	0.9074	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:02	FANDSRNML	0.9986	0.9815	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C01:02	FANDSRNML	0.9986	0.9389	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C16:04	FANDSRNML	0.9983	0.9894	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B38:05	SRNMLGFTL	0.9981	0.9903	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C12:03	FANDSRNML	0.998	0.9816	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:06	FANDSRNML	0.9975	0.9891	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:17	SRNMLGFTL	0.9967	0.9745	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:02	FANDSRNML	0.9964	0.9953	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:17	FANDSRNML	0.9964	0.9953	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C12:02	FANDSRNML	0.9959	0.9828	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C04:04	FANDSRNML	0.9957	0.8542	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:06	FANDSRNML	0.993	0.994	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C16:01	FANDSRNML	0.9922	0.9807	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:04	FANDSRNML	0.9907	0.9149	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B07:13	FANDSRNML	0.988	0.8658	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C16:02	FANDSRNML	0.9862	0.9918	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:08	SRNMLGFTL	0.9857	0.9906	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:13	FANDSRNML	0.9798	0.9521	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C02:10	FANDSRNML	0.9761	0.9894	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C02:02	FANDSRNML	0.9761	0.9894	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C08:01	FANDSRNML	0.9644	0.9887	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:11	FANDSRNML	0.9643	0.9564	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:08	FANDSRNML	0.9593	0.9927	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:22	FANDSRNML	0.9529	0.8495	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:02	SRNMLGFTL	0.9403	0.9671	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:17	FANDSRNML	0.9403	0.9805	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:04	SRNMLGFTL	0.9389	0.9729	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:09	SRNMLGFTL	0.9234	0.6681	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B08:12	FANDSRNML	0.9144	0.7074	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:09	FANDSRNML	0.8975	0.969	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:08	FANDSRNML	0.8873	0.9346	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:01	FANDSRNML	0.8822	0.6645	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B35:43	FANDSRNML	0.8753	0.9335	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C07:22	SRNMLGFTL	0.8477	0.8463	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:11	SRNMLGFTL	0.7989	0.8728	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:67	SRNMLGFTL	0.697	0.9858	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B78:02	FANDSRNML	0.601	0.7085	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C17:01	FANDSRNML	0.5528	0.9134	5	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:06	SRNMLGFTL	0.5431	0.9948	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:17	SRNMLGFTL	0.1751	0.9958	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C06:02	SRNMLGFTL	0.1751	0.9958	9	18
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C03:02	FANDSRNMLGF	1	0.9692	5	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:31	FHFANDSRNML	0.9999	0.8824	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B38:05	FHFANDSRNML	0.9996	0.9197	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C02:02	FANDSRNMLGF	0.9989	0.9859	5	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-C02:10	FANDSRNMLGF	0.9989	0.9859	5	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B15:09	FHFANDSRNML	0.9976	0.7157	3	14
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	HLA-B39:11	FHFANDSRNML	0.9839	0.8024	3	14

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Potential FusionNeoAntigen Information of ZP3-C7orf73 in HLA II

ZP3-C7orf73_76063472_135357500.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0305	VFHFANDSRNMLGFT	2	17
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0305	DVFHFANDSRNMLGF	1	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0305	VDVFHFANDSRNMLG	0	15
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0338	VFHFANDSRNMLGFT	2	17
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0338	DVFHFANDSRNMLGF	1	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0340	VFHFANDSRNMLGFT	2	17
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0340	DVFHFANDSRNMLGF	1	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB1-0340	VDVFHFANDSRNMLG	0	15
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB3-0114	VFHFANDSRNMLGFT	2	17
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB3-0114	DVFHFANDSRNMLGF	1	16
ZP3-C7orf73	chr7	76063472	chr7	135357500	1024	DRB3-0114	VDVFHFANDSRNMLG	0	15

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Fusion breakpoint peptide structures of ZP3-C7orf73

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
443	ANDSRNMLGFTLGN	ZP3	C7orf73	chr7	76063472	chr7	135357500	1024

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ZP3-C7orf73

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	443	ANDSRNMLGFTLGN	-7.9962	-8.1096
HLA-B14:02	3BVN	443	ANDSRNMLGFTLGN	-5.70842	-6.74372
HLA-B52:01	3W39	443	ANDSRNMLGFTLGN	-6.83737	-6.95077
HLA-B52:01	3W39	443	ANDSRNMLGFTLGN	-4.4836	-5.5189
HLA-A11:01	4UQ2	443	ANDSRNMLGFTLGN	-10.0067	-10.1201
HLA-A11:01	4UQ2	443	ANDSRNMLGFTLGN	-9.03915	-10.0745
HLA-A24:02	5HGA	443	ANDSRNMLGFTLGN	-6.56204	-6.67544
HLA-A24:02	5HGA	443	ANDSRNMLGFTLGN	-5.42271	-6.45801
HLA-B44:05	3DX8	443	ANDSRNMLGFTLGN	-7.85648	-8.89178
HLA-B44:05	3DX8	443	ANDSRNMLGFTLGN	-5.3978	-5.5112
HLA-A02:01	6TDR	443	ANDSRNMLGFTLGN	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of ZP3-C7orf73

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
ZP3-C7orf73	chr7	76063472	chr7	135357500	3	14	FHFANDSRNML	TTCCACTTTGCTAATGACTCCAGAAACATGCTT
ZP3-C7orf73	chr7	76063472	chr7	135357500	5	14	FANDSRNML	TTTGCTAATGACTCCAGAAACATGCTT
ZP3-C7orf73	chr7	76063472	chr7	135357500	5	16	FANDSRNMLGF	TTTGCTAATGACTCCAGAAACATGCTTGGATTT
ZP3-C7orf73	chr7	76063472	chr7	135357500	9	18	SRNMLGFTL	TCCAGAAACATGCTTGGATTTACACTG

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
ZP3-C7orf73	chr7	76063472	chr7	135357500	0	15	VDVFHFANDSRNMLG	GTGGATGTCTTCCACTTTGCTAATGACTCCAGAAACATGCTTGGA
ZP3-C7orf73	chr7	76063472	chr7	135357500	1	16	DVFHFANDSRNMLGF	GATGTCTTCCACTTTGCTAATGACTCCAGAAACATGCTTGGATTT
ZP3-C7orf73	chr7	76063472	chr7	135357500	2	17	VFHFANDSRNMLGFT	GTCTTCCACTTTGCTAATGACTCCAGAAACATGCTTGGATTTACA

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Information of the samples that have these potential fusion neoantigens of ZP3-C7orf73

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
STAD	ZP3-C7orf73	chr7	76063472	ENST00000336517	chr7	135357500	ENST00000507606	TCGA-BR-8485

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Potential target of CAR-T therapy development for ZP3-C7orf73

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

C7orf73

chr7:76063472

chr7:135357500

ENST00000507606

7_23

48.0

Transmembrane

Helical

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to ZP3-C7orf73

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to ZP3-C7orf73

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)