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Fusion Protein:CHAF1A-ANKRD24 |
Fusion Gene and Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: CHAF1A-ANKRD24 | FusionPDB ID: 16202 | FusionGDB2.0 ID: 16202 | Hgene | Tgene | Gene symbol | CHAF1A | ANKRD24 | Gene ID | 10036 | 170961 |
Gene name | chromatin assembly factor 1 subunit A | ankyrin repeat domain 24 | |
Synonyms | CAF-1|CAF1|CAF1B|CAF1P150|P150 | - | |
Cytomap | 19p13.3 | 19p13.3 | |
Type of gene | protein-coding | protein-coding | |
Description | chromatin assembly factor 1 subunit ACAF-1 subunit ACAF-I 150 kDa subunitCAF-I p150CTB-50L17.7chromatin assembly factor I (150 kDa)chromatin assembly factor I p150 subunithp150 | ankyrin repeat domain-containing protein 24 | |
Modification date | 20200313 | 20200313 | |
UniProtAcc | Q13111 Main function of 5'-partner protein: FUNCTION: Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. {ECO:0000250|UniProtKB:Q5R1T0}. | Q8TF21 Main function of 5'-partner protein: | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000301280, ENST00000587368, | ENST00000595096, ENST00000262970, ENST00000318934, ENST00000600132, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 5 X 6 X 3=90 | 7 X 7 X 7=343 |
# samples | 5 | 9 | |
** MAII score | log2(5/90*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(9/343*10)=-1.93021166984314 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Fusion gene context | PubMed: CHAF1A [Title/Abstract] AND ANKRD24 [Title/Abstract] AND fusion [Title/Abstract] | ||
Fusion neoantigen context | PubMed: CHAF1A [Title/Abstract] AND ANKRD24 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | CHAF1A(4409756)-ANKRD24(4210055), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. | CHAF1A-ANKRD24 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CHAF1A-ANKRD24 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CHAF1A-ANKRD24 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CHAF1A-ANKRD24 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | CHAF1A | GO:0006335 | DNA replication-dependent nucleosome assembly | 14718166 |
Hgene | CHAF1A | GO:0031497 | chromatin assembly | 8858152 |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:4409756/chr19:4210055) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000301280 | CHAF1A | chr19 | 4409756 | + | ENST00000600132 | ANKRD24 | chr19 | 4210055 | + | 3941 | 1061 | 71 | 3631 | 1186 |
ENST00000301280 | CHAF1A | chr19 | 4409756 | + | ENST00000318934 | ANKRD24 | chr19 | 4210055 | + | 3941 | 1061 | 71 | 3631 | 1186 |
ENST00000301280 | CHAF1A | chr19 | 4409756 | + | ENST00000262970 | ANKRD24 | chr19 | 4210055 | + | 3632 | 1061 | 71 | 3631 | 1187 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000301280 | ENST00000600132 | CHAF1A | chr19 | 4409756 | + | ANKRD24 | chr19 | 4210055 | + | 0.010484247 | 0.9895158 |
ENST00000301280 | ENST00000318934 | CHAF1A | chr19 | 4409756 | + | ANKRD24 | chr19 | 4210055 | + | 0.010484247 | 0.9895158 |
ENST00000301280 | ENST00000262970 | CHAF1A | chr19 | 4409756 | + | ANKRD24 | chr19 | 4210055 | + | 0.012532192 | 0.98746777 |
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Get the fusion protein sequences from here. |
Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for CHAF1A-ANKRD24 |
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Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
CHAF1A | chr19 | 4409756 | ANKRD24 | chr19 | 4210055 | 1061 | 330 | STSPFPTSTPLRRNSMSSHGKQGAPK |
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Potential FusionNeoAntigen Information of CHAF1A-ANKRD24 in HLA I |
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CHAF1A-ANKRD24_4409756_4210055.msa |
![]() * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B08:09 | TPLRRNSM | 0.9961 | 0.5392 | 8 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B27:05 | RRNSMSSHGK | 0.999 | 0.7708 | 11 | 21 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B07:12 | TPLRRNSM | 0.998 | 0.6232 | 8 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B14:03 | STPLRRNSM | 0.8034 | 0.8613 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-C01:17 | STPLRRNSM | 0.6916 | 0.9662 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-C01:30 | STPLRRNSM | 0.6112 | 0.9718 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B27:14 | RRNSMSSHGK | 0.9985 | 0.6949 | 11 | 21 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B27:03 | RRNSMSSHGK | 0.9875 | 0.793 | 11 | 21 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B07:09 | TPLRRNSM | 0.9991 | 0.5176 | 8 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B08:12 | TPLRRNSM | 0.9012 | 0.6366 | 8 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-C03:17 | STPLRRNSM | 0.9483 | 0.96 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B08:12 | STPLRRNSM | 0.8914 | 0.6699 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-C01:02 | STPLRRNSM | 0.7255 | 0.9636 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-C16:01 | STPLRRNSM | 0.6052 | 0.9817 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-C01:03 | STPLRRNSM | 0.484 | 0.9551 | 7 | 16 |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 | HLA-B27:10 | RRNSMSSHGK | 0.9982 | 0.7677 | 11 | 21 |
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Potential FusionNeoAntigen Information of CHAF1A-ANKRD24 in HLA II |
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![]() * We used NetMHCIIpan v4.1 (%rank<0.5). |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of CHAF1A-ANKRD24 |
![]() * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
9677 | TSTPLRRNSMSSHG | CHAF1A | ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 1061 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of CHAF1A-ANKRD24 |
![]() * We used Glide to predict the interaction between HLAs and neoantigens. |
HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
HLA-B14:02 | 3BVN | 9677 | TSTPLRRNSMSSHG | -6.00367 | -7.03897 |
HLA-B14:02 | 3BVN | 9677 | TSTPLRRNSMSSHG | -5.39279 | -5.50619 |
HLA-B52:01 | 3W39 | 9677 | TSTPLRRNSMSSHG | -6.37513 | -6.48853 |
HLA-B52:01 | 3W39 | 9677 | TSTPLRRNSMSSHG | -5.71942 | -6.75472 |
HLA-A11:01 | 4UQ2 | 9677 | TSTPLRRNSMSSHG | -11.5708 | -11.6842 |
HLA-A11:01 | 4UQ2 | 9677 | TSTPLRRNSMSSHG | -8.11091 | -9.14621 |
HLA-A24:02 | 5HGA | 9677 | TSTPLRRNSMSSHG | -6.75661 | -6.87001 |
HLA-A24:02 | 5HGA | 9677 | TSTPLRRNSMSSHG | -5.30147 | -6.33677 |
HLA-B27:05 | 6PYJ | 9677 | TSTPLRRNSMSSHG | -4.27108 | -5.30638 |
HLA-B44:05 | 3DX8 | 9677 | TSTPLRRNSMSSHG | -6.47731 | -6.59071 |
HLA-B44:05 | 3DX8 | 9677 | TSTPLRRNSMSSHG | -3.23433 | -4.26963 |
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Vaccine Design for the FusionNeoAntigens of CHAF1A-ANKRD24 |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 11 | 21 | RRNSMSSHGK | CGCAGAAACTCTATGTCCAGCCATGGAAAG |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 7 | 16 | STPLRRNSM | TCCACGCCCCTCCGCAGAAACTCTATG |
CHAF1A-ANKRD24 | chr19 | 4409756 | chr19 | 4210055 | 8 | 16 | TPLRRNSM | ACGCCCCTCCGCAGAAACTCTATG |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of CHAF1A-ANKRD24 |
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Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
STAD | CHAF1A-ANKRD24 | chr19 | 4409756 | ENST00000301280 | chr19 | 4210055 | ENST00000262970 | TCGA-CD-5813-01A |
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Potential target of CAR-T therapy development for CHAF1A-ANKRD24 |
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![]() * Minus value of BPloci means that the break point is located before the CDS. |
- In-frame and retained 'Transmembrane'. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
![]() * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to CHAF1A-ANKRD24 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to CHAF1A-ANKRD24 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |