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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:DHRS12-RASL11A

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: DHRS12-RASL11A
FusionPDB ID: 22566
FusionGDB2.0 ID: 22566
HgeneTgene
Gene symbol

DHRS12

RASL11A

Gene ID

79758

387496

Gene namedehydrogenase/reductase 12RAS like family 11 member A
SynonymsSDR40C1-
Cytomap

13q14.3

13q12.2

Type of geneprotein-codingprotein-coding
Descriptiondehydrogenase/reductase SDR family member 12dehydrogenase/reductase (SDR family) member 12short-chain dehydrogenase/reductase family 40C member 1ras-like protein family member 11A
Modification date2020031320200313
UniProtAcc

A0PJE2

Main function of 5'-partner protein: FUNCTION: Putative oxidoreductase. {ECO:0000250}.		.
Ensembl transtripts involved in fusion geneENST idsENST00000218981, ENST00000280056, 
ENST00000444610, ENST00000490949, 
ENST00000480803, ENST00000241463, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score6 X 7 X 4=1683 X 2 X 3=18
# samples 74
** MAII scorelog2(7/168*10)=-1.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(4/18*10)=1.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Fusion gene context

PubMed: DHRS12 [Title/Abstract] AND RASL11A [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: DHRS12 [Title/Abstract] AND RASL11A [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)DHRS12(52373670)-RASL11A(27845621), # samples:3
RASL11A(27845205)-DHRS12(52365393), # samples:2
Anticipated loss of major functional domain due to fusion event.DHRS12-RASL11A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DHRS12-RASL11A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
DHRS12-RASL11A seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
RASL11A-DHRS12 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr13:52373670/chr13:27845621)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across DHRS12 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across RASL11A (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000444610DHRS12chr1352373670-ENST00000241463RASL11Achr1327845621+100520414808264
ENST00000444610DHRS12chr1352373669-ENST00000241463RASL11Achr1327845620+100520414808264

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000444610ENST00000241463DHRS12chr1352373670-RASL11Achr1327845621+0.0064320480.993568
ENST00000444610ENST00000241463DHRS12chr1352373669-RASL11Achr1327845620+0.0064320480.993568

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for DHRS12-RASL11A

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
DHRS12chr1352373669RASL11Achr132784562020463GIGKATALEIAKRAMIVRFLTKRFIG
DHRS12chr1352373670RASL11Achr132784562120463GIGKATALEIAKRAMIVRFLTKRFIG

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Potential FusionNeoAntigen Information of DHRS12-RASL11A in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
DHRS12-RASL11A_52373669_27845620.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:05KRAMIVRF10.82081119
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:04KRAMIVRF0.99990.64931119
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B18:01LEIAKRAM0.99830.9351715
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:04KRAMIVRFL0.99980.63071120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:05KRAMIVRFL0.99980.82551120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B08:09EIAKRAMIV0.99540.7513817
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B08:01EIAKRAMIV0.99510.5743817
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B15:03AKRAMIVRF0.25520.70261019
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A30:08RAMIVRFLTK0.99610.59471222
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A33:05EIAKRAMIVR0.96960.5203818
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A33:01EIAKRAMIVR0.96960.5203818
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:05KRAMIVRFLTK0.99990.85751122
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A31:02RAMIVRFLTKR0.94680.60011223
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:05KRAMIVRF0.99980.95231119
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:95KRAMIVRF0.99980.71111119
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:03KRAMIVRF0.9990.851119
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:14KRAMIVRFL0.99980.76111120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:95KRAMIVRFL0.99780.63611120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:03KRAMIVRFL0.99530.85331120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:05KRAMIVRFL0.9890.94311120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:27KRAMIVRFL0.98690.93431120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:19KRAMIVRFL0.97930.59131120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:29KRAMIVRFL0.97620.90941120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:13KRAMIVRFL0.97070.82691120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:46KRAMIVRFL0.96710.78421120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:80KRAMIVRFL0.95660.91441120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:67KRAMIVRFL0.95660.91441120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:10KRAMIVRFL0.95570.91461120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A31:01IAKRAMIVR0.93490.6154918
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C12:16KRAMIVRFL0.03780.96621120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:14KRAMIVRFLTK0.99980.77351122
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:03KRAMIVRFLTK0.99740.87481122
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A31:01RAMIVRFLTKR0.98430.54511223
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:01KRAMIVRF0.99990.65991119
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:10KRAMIVRF0.99990.84261119
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B18:06LEIAKRAM0.99840.9532715
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B18:05LEIAKRAM0.99830.9351715
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B18:03LEIAKRAM0.9970.9286715
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C16:02RAMIVRFL0.99480.99261220
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C16:01RAMIVRFL0.98960.98061220
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B18:11LEIAKRAM0.98720.8536715
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:08KRAMIVRFL0.99980.66371120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:06KRAMIVRFL0.99980.61391120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:10KRAMIVRFL0.99980.82511120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:09KRAMIVRFL0.99960.78691120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:01KRAMIVRFL0.99830.57751120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B08:18EIAKRAMIV0.99510.5743817
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A68:02EIAKRAMIV0.99290.7721817
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:17KRAMIVRFL0.96190.97511120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:02KRAMIVRFL0.95660.91441120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C06:08KRAMIVRFL0.94740.99031120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:04KRAMIVRFL0.85340.88681120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C07:22KRAMIVRFL0.79850.67041120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C06:06KRAMIVRFL0.33720.98281120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C06:02KRAMIVRFL0.12020.99511120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-C06:17KRAMIVRFL0.12020.99511120
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B15:68AKRAMIVRF0.10520.68331019
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B15:54AKRAMIVRF0.03660.81851019
DHRS12-RASL11Achr1352373669chr1327845620204HLA-A30:01RAMIVRFLTK0.99590.74691222
DHRS12-RASL11Achr1352373669chr1327845620204HLA-B27:10KRAMIVRFLTK0.99980.83741122

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Potential FusionNeoAntigen Information of DHRS12-RASL11A in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
DHRS12-RASL11A_52373669_27845620.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1113KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1113ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1117KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1117ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1117GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1152KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1152ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1152GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1189KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1189ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1189GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1377KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1401KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1401ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1401GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1404KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1404ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1404GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1405KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1405ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1405GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1407KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1407ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1407GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1408KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1408ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1408GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1411KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1411ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1414KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1414ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1418KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1418ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1418GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1423KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1423ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1423GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1426KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1426ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1426GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1428KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1428ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1428GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1431KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1431ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1432KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1432ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1432GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1434KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1434ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1435KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1435ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1435GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1436KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1436ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1438KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1438ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1438GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1439KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1439ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1442KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1442ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1443KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1443ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1443GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1444KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1444ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1445KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1445ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1445GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1450KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1450ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1450GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1454KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1454ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1454GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1455KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1455ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1455GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1456KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1456ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1456GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1458KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1458ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1458GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1459KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1459ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1459GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1460KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1460ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1460GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1461KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1461ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1461GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1462KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1462ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1462GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1464KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1464ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1464GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1465KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1468KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1468ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1470KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1470ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1470GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1471KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1471ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1471GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1472KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1472ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1472GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1475KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1475ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1475GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1481KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1481ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1482KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1482ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1482GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1486KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1486ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1486GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1487KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1487ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1487GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1488KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1488ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1488GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1490KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1490ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1490GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1491KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1491ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1491GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1493KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1495KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1495ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1496KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1496ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1496GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1497KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1497ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1497GKATALEIAKRAMIV217
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1499KATALEIAKRAMIVR318
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1499ATALEIAKRAMIVRF419
DHRS12-RASL11Achr1352373669chr1327845620204DRB1-1499GKATALEIAKRAMIV217

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Fusion breakpoint peptide structures of DHRS12-RASL11A

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
351ALEIAKRAMIVRFLDHRS12RASL11Achr1352373669chr1327845620204

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of DHRS12-RASL11A

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN351ALEIAKRAMIVRFL-7.15543-7.26883
HLA-B14:023BVN351ALEIAKRAMIVRFL-4.77435-5.80965
HLA-B52:013W39351ALEIAKRAMIVRFL-6.80875-6.92215
HLA-B52:013W39351ALEIAKRAMIVRFL-4.20386-5.23916
HLA-A11:014UQ2351ALEIAKRAMIVRFL-7.5194-8.5547
HLA-A11:014UQ2351ALEIAKRAMIVRFL-6.9601-7.0735
HLA-A24:025HGA351ALEIAKRAMIVRFL-7.52403-7.63743
HLA-A24:025HGA351ALEIAKRAMIVRFL-5.82433-6.85963
HLA-B27:056PYJ351ALEIAKRAMIVRFL-3.28285-4.31815
HLA-B44:053DX8351ALEIAKRAMIVRFL-5.91172-6.94702
HLA-B44:053DX8351ALEIAKRAMIVRFL-4.24346-4.35686

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Vaccine Design for the FusionNeoAntigens of DHRS12-RASL11A

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
DHRS12-RASL11Achr1352373669chr13278456201019AKRAMIVRFCCAAGCGAGCAATGATCGTGCGCTTCC
DHRS12-RASL11Achr1352373669chr13278456201119KRAMIVRFAGCGAGCAATGATCGTGCGCTTCC
DHRS12-RASL11Achr1352373669chr13278456201120KRAMIVRFLAGCGAGCAATGATCGTGCGCTTCCTGA
DHRS12-RASL11Achr1352373669chr13278456201122KRAMIVRFLTKAGCGAGCAATGATCGTGCGCTTCCTGACCAAGA
DHRS12-RASL11Achr1352373669chr13278456201220RAMIVRFLGAGCAATGATCGTGCGCTTCCTGA
DHRS12-RASL11Achr1352373669chr13278456201222RAMIVRFLTKGAGCAATGATCGTGCGCTTCCTGACCAAGA
DHRS12-RASL11Achr1352373669chr13278456201223RAMIVRFLTKRGAGCAATGATCGTGCGCTTCCTGACCAAGAGAT
DHRS12-RASL11Achr1352373669chr1327845620715LEIAKRAMTTGAAATCGCCAAGCGAGCAATGA
DHRS12-RASL11Achr1352373669chr1327845620817EIAKRAMIVAAATCGCCAAGCGAGCAATGATCGTGC
DHRS12-RASL11Achr1352373669chr1327845620818EIAKRAMIVRAAATCGCCAAGCGAGCAATGATCGTGCGCT
DHRS12-RASL11Achr1352373669chr1327845620918IAKRAMIVRTCGCCAAGCGAGCAATGATCGTGCGCT

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
DHRS12-RASL11Achr1352373669chr1327845620217GKATALEIAKRAMIVGCAAAGCAACTGCCCTTGAAATCGCCAAGCGAGCAATGATCGTGC
DHRS12-RASL11Achr1352373669chr1327845620318KATALEIAKRAMIVRAAGCAACTGCCCTTGAAATCGCCAAGCGAGCAATGATCGTGCGCT
DHRS12-RASL11Achr1352373669chr1327845620419ATALEIAKRAMIVRFCAACTGCCCTTGAAATCGCCAAGCGAGCAATGATCGTGCGCTTCC

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Information of the samples that have these potential fusion neoantigens of DHRS12-RASL11A

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BLCADHRS12-RASL11Achr1352373669ENST00000444610chr1327845620ENST00000241463TCGA-GC-A3RC

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Potential target of CAR-T therapy development for DHRS12-RASL11A

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to DHRS12-RASL11A

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to DHRS12-RASL11A

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource