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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:DHRS4-SCFD1

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: DHRS4-SCFD1
FusionPDB ID: 22591
FusionGDB2.0 ID: 22591
HgeneTgene
Gene symbol

DHRS4

SCFD1

Gene ID

10901

23256

Gene namedehydrogenase/reductase 4sec1 family domain containing 1
SynonymsCR|NRDR|PHCR|PSCD|SCAD-SRL|SDR-SRL|SDR25C1|SDR25C2C14orf163|RA410|SLY1|SLY1P|STXBP1L2
Cytomap

14q11.2

14q12

Type of geneprotein-codingprotein-coding
Descriptiondehydrogenase/reductase SDR family member 4NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenaseNADPH-dependent retinol dehydrogenase/reductasedehydrogenase/reductase (SDR family) member 4 like 2Aperoxisomal short-chain alcohol dehydrogenasessec1 family domain-containing protein 1syntaxin-binding protein 1-like 2vesicle transport-related protein
Modification date2020031320200313
UniProtAcc

Q6PKH6

Main function of 5'-partner protein: FUNCTION: Probable oxidoreductase. {ECO:0000250}.		.
Ensembl transtripts involved in fusion geneENST idsENST00000308178, ENST00000313250, 
ENST00000382761, ENST00000397073, 
ENST00000397074, ENST00000397075, 
ENST00000421831, ENST00000543741, 
ENST00000558263, ENST00000558581, 
ENST00000559632, 		ENST00000396629, 
ENST00000541123, ENST00000554486, 
ENST00000421551, ENST00000458591, 
ENST00000544052, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score5 X 3 X 3=458 X 8 X 5=320
# samples 59
** MAII scorelog2(5/45*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0		log2(9/320*10)=-1.83007499855769
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: DHRS4 [Title/Abstract] AND SCFD1 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: DHRS4 [Title/Abstract] AND SCFD1 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)DHRS4(24423125)-SCFD1(31103153), # samples:3
Anticipated loss of major functional domain due to fusion event.DHRS4-SCFD1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DHRS4-SCFD1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DHRS4-SCFD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
DHRS4-SCFD1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneDHRS4

GO:0006066

alcohol metabolic process

18571493

HgeneDHRS4

GO:0008202

steroid metabolic process

18571493

HgeneDHRS4

GO:0042180

cellular ketone metabolic process

18571493

HgeneDHRS4

GO:0055114

oxidation-reduction process

19442656



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr14:24423125/chr14:31103153)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across DHRS4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across SCFD1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000313250DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+22423312032038611
ENST00000313250DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+21223312032038611
ENST00000313250DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+22193312032038611
ENST00000558263DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+2094183551890611
ENST00000558263DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+1974183551890611
ENST00000558263DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+2071183551890611
ENST00000543741DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+2058147191854611
ENST00000543741DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+1938147191854611
ENST00000543741DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+2035147191854611
ENST00000382761DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+2058147191854611
ENST00000382761DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+1938147191854611
ENST00000382761DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+2035147191854611
ENST00000397073DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+2058147191854611
ENST00000397073DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+1938147191854611
ENST00000397073DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+2035147191854611
ENST00000308178DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+2058147191854611
ENST00000308178DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+1938147191854611
ENST00000308178DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+2035147191854611
ENST00000421831DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+2058147191854611
ENST00000421831DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+1938147191854611
ENST00000421831DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+2035147191854611
ENST00000397075DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+2049138101845611
ENST00000397075DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+1929138101845611
ENST00000397075DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+2026138101845611
ENST00000397074DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+204213131838611
ENST00000397074DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+192213131838611
ENST00000397074DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+201913131838611
ENST00000559632DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+203912801835611
ENST00000559632DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+191912801835611
ENST00000559632DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+201612801835611
ENST00000558581DHRS4chr1424423125+ENST00000458591SCFD1chr1431103153+203912801835611
ENST00000558581DHRS4chr1424423125+ENST00000421551SCFD1chr1431103153+191912801835611
ENST00000558581DHRS4chr1424423125+ENST00000544052SCFD1chr1431103153+201612801835611

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000313250ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0006045610.9993955
ENST00000313250ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008502170.9991498
ENST00000313250ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.000601650.99939835
ENST00000558263ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005459230.9994541
ENST00000558263ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.000788990.99921095
ENST00000558263ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005429220.99945706
ENST00000543741ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005683940.99943155
ENST00000543741ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008448440.99915516
ENST00000543741ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005655170.9994344
ENST00000382761ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005683940.99943155
ENST00000382761ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008448440.99915516
ENST00000382761ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005655170.9994344
ENST00000397073ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005683940.99943155
ENST00000397073ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008448440.99915516
ENST00000397073ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005655170.9994344
ENST00000308178ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005683940.99943155
ENST00000308178ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008448440.99915516
ENST00000308178ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005655170.9994344
ENST00000421831ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005683940.99943155
ENST00000421831ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008448440.99915516
ENST00000421831ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005655170.9994344
ENST00000397075ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.000565720.9994343
ENST00000397075ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.000830020.99917006
ENST00000397075ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005635790.99943644
ENST00000397074ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005708440.99942917
ENST00000397074ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008344580.99916553
ENST00000397074ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005687370.9994312
ENST00000559632ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005738890.9994261
ENST00000559632ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008304890.9991696
ENST00000559632ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005717990.9994282
ENST00000558581ENST00000458591DHRS4chr1424423125+SCFD1chr1431103153+0.0005738890.9994261
ENST00000558581ENST00000421551DHRS4chr1424423125+SCFD1chr1431103153+0.0008304890.9991696
ENST00000558581ENST00000544052DHRS4chr1424423125+SCFD1chr1431103153+0.0005717990.9994282

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for DHRS4-SCFD1

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
DHRS4chr1424423125SCFD1chr143110315312843ANKVALVTASTDGLLHSDRDPIPDVP
DHRS4chr1424423125SCFD1chr143110315313143ANKVALVTASTDGLLHSDRDPIPDVP
DHRS4chr1424423125SCFD1chr143110315313843ANKVALVTASTDGLLHSDRDPIPDVP
DHRS4chr1424423125SCFD1chr143110315314743ANKVALVTASTDGLLHSDRDPIPDVP
DHRS4chr1424423125SCFD1chr143110315318343ANKVALVTASTDGLLHSDRDPIPDVP
DHRS4chr1424423125SCFD1chr143110315333143ANKVALVTASTDGLLHSDRDPIPDVP

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Potential FusionNeoAntigen Information of DHRS4-SCFD1 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Potential FusionNeoAntigen Information of DHRS4-SCFD1 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
DHRS4-SCFD1_24423125_31103153.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1117TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1117STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1152TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1152STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1401TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1401STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1401ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1404TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1404STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1405TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1405STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1407TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1407STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1408TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1408STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1418TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1423TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1423STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1426TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1426STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1426ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1428TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1428STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1432TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1432STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1435TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1435STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1438TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1438STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1438ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1439TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1439STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1443TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1443STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1445TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1450TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1450STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1454TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1454STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1454ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1456TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1456STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1458TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1458STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1458ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1459TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1459STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1460TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1460STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1460ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1461TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1461STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1462TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1462STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1462ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1468TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1470TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1470STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1470ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1471TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1471STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1475TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1475STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1475ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1475DGLLHSDRDPIPDVP1126
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1482TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1482STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1482ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1482DGLLHSDRDPIPDVP1126
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1486TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1486STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1486ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1487TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1487STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1487ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1488TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1488STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1488ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1490TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1490STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1490ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1491TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1491STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1493TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1495TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1495STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1496TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1496STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1497TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1497STDGLLHSDRDPIPD924
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1497ASTDGLLHSDRDPIP823
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1499TDGLLHSDRDPIPDV1025
DHRS4-SCFD1chr1424423125chr1431103153147DRB1-1499STDGLLHSDRDPIPD924

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Fusion breakpoint peptide structures of DHRS4-SCFD1

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of DHRS4-SCFD1

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score

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Vaccine Design for the FusionNeoAntigens of DHRS4-SCFD1

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
DHRS4-SCFD1chr1424423125chr14311031531025TDGLLHSDRDPIPDVCACCGACGGGCTTTTACACTCTGATCGAGATCCTATTCCAGATGT
DHRS4-SCFD1chr1424423125chr14311031531126DGLLHSDRDPIPDVPCGACGGGCTTTTACACTCTGATCGAGATCCTATTCCAGATGTTCC
DHRS4-SCFD1chr1424423125chr1431103153823ASTDGLLHSDRDPIPGGCCTCCACCGACGGGCTTTTACACTCTGATCGAGATCCTATTCC
DHRS4-SCFD1chr1424423125chr1431103153924STDGLLHSDRDPIPDCTCCACCGACGGGCTTTTACACTCTGATCGAGATCCTATTCCAGA

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Information of the samples that have these potential fusion neoantigens of DHRS4-SCFD1

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample

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Potential target of CAR-T therapy development for DHRS4-SCFD1

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to DHRS4-SCFD1

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to DHRS4-SCFD1

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource