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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:DLG1-MYO9B

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: DLG1-MYO9B
FusionPDB ID: 23043
FusionGDB2.0 ID: 23043
HgeneTgene
Gene symbol

DLG1

MYO9B

Gene ID

1739

4650

Gene namediscs large MAGUK scaffold protein 1myosin IXB
SynonymsDLGH1|SAP-97|SAP97|hdlgCELIAC4|MYR5
Cytomap

3q29

19p13.11

Type of geneprotein-codingprotein-coding
Descriptiondisks large homolog 1dJ1061C18.1.1discs large homolog 1, scribble cell polarity complex componentpresynaptic protein SAP97synapse-associated protein 97unconventional myosin-IXbmyosin-IXbunconventional myosin-9b
Modification date2020032720200313
UniProtAcc

Q12959

Main function of 5'-partner protein: FUNCTION: Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane (PubMed:23676497). {ECO:0000250, ECO:0000269|PubMed:10656683, ECO:0000269|PubMed:12445884, ECO:0000269|PubMed:14699157, ECO:0000269|PubMed:15263016, ECO:0000269|PubMed:19213956, ECO:0000269|PubMed:20605917, ECO:0000269|PubMed:23676497}.

Q13459

Main function of 5'-partner protein: FUNCTION: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions (PubMed:9490638). Also acts as a GTPase activator for RHOA (PubMed:9490638, PubMed:26529257). Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). {ECO:0000269|PubMed:26529257, ECO:0000269|PubMed:9490638}.
Ensembl transtripts involved in fusion geneENST idsENST00000314062, ENST00000346964, 
ENST00000357674, ENST00000392382, 
ENST00000419354, ENST00000422288, 
ENST00000448528, ENST00000450955, 
ENST00000443183, ENST00000452595, 
ENST00000485409, 		ENST00000593411, 
ENST00000397274, ENST00000594824, 
ENST00000595618, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score27 X 18 X 14=680415 X 18 X 8=2160
# samples 4018
** MAII scorelog2(40/6804*10)=-4.08831123588866
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(18/2160*10)=-3.58496250072116
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: DLG1 [Title/Abstract] AND MYO9B [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: DLG1 [Title/Abstract] AND MYO9B [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)DLG1(196921296)-MYO9B(17301906), # samples:3
Anticipated loss of major functional domain due to fusion event.DLG1-MYO9B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DLG1-MYO9B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DLG1-MYO9B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
DLG1-MYO9B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneDLG1

GO:0001935

endothelial cell proliferation

14699157

HgeneDLG1

GO:0007015

actin filament organization

14699157

HgeneDLG1

GO:0030866

cortical actin cytoskeleton organization

14699157

HgeneDLG1

GO:0042391

regulation of membrane potential

12970345

HgeneDLG1

GO:0043268

positive regulation of potassium ion transport

12970345

HgeneDLG1

GO:0070830

bicellular tight junction assembly

17332497

HgeneDLG1

GO:0098609

cell-cell adhesion

14699157

HgeneDLG1

GO:1902473

regulation of protein localization to synapse

23676497

HgeneDLG1

GO:1903078

positive regulation of protein localization to plasma membrane

12970345

TgeneMYO9B

GO:0030048

actin filament-based movement

9490638

TgeneMYO9B

GO:0032011

ARF protein signal transduction

15644318

TgeneMYO9B

GO:0035385

Roundabout signaling pathway

26529257



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr3:196921296/chr19:17301906)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across DLG1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across MYO9B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000357674DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+545667319040531287
ENST00000357674DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+543367319044581422
ENST00000357674DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+545567319040531287
ENST00000314062DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+545667319040531287
ENST00000314062DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+543367319044581422
ENST00000314062DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+545567319040531287
ENST00000346964DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+545667319040531287
ENST00000346964DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+543367319044581422
ENST00000346964DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+545567319040531287
ENST00000419354DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+555377028741501287
ENST00000419354DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+553077028745551422
ENST00000419354DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+555277028741501287
ENST00000422288DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+5266483038631287
ENST00000422288DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+5243483042681422
ENST00000422288DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+5265483038631287
ENST00000448528DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+545667319040531287
ENST00000448528DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+543367319044581422
ENST00000448528DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+545567319040531287
ENST00000450955DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+5266483038631287
ENST00000450955DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+5243483042681422
ENST00000450955DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+5265483038631287
ENST00000392382DLG1chr3196921296-ENST00000595618MYO9Bchr1917301906+53405577439371287
ENST00000392382DLG1chr3196921296-ENST00000594824MYO9Bchr1917301906+53175577443421422
ENST00000392382DLG1chr3196921296-ENST00000397274MYO9Bchr1917301906+53395577439371287
ENST00000357674DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+545667319040531287
ENST00000357674DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+543367319044581422
ENST00000357674DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+545567319040531287
ENST00000314062DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+545667319040531287
ENST00000314062DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+543367319044581422
ENST00000314062DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+545567319040531287
ENST00000346964DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+545667319040531287
ENST00000346964DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+543367319044581422
ENST00000346964DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+545567319040531287
ENST00000419354DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+555377028741501287
ENST00000419354DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+553077028745551422
ENST00000419354DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+555277028741501287
ENST00000422288DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+5266483038631287
ENST00000422288DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+5243483042681422
ENST00000422288DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+5265483038631287
ENST00000448528DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+545667319040531287
ENST00000448528DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+543367319044581422
ENST00000448528DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+545567319040531287
ENST00000450955DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+5266483038631287
ENST00000450955DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+5243483042681422
ENST00000450955DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+5265483038631287
ENST00000392382DLG1chr3196921295-ENST00000595618MYO9Bchr1917301905+53405577439371287
ENST00000392382DLG1chr3196921295-ENST00000594824MYO9Bchr1917301905+53175577443421422
ENST00000392382DLG1chr3196921295-ENST00000397274MYO9Bchr1917301905+53395577439371287

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000357674ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.0035093950.99649054
ENST00000357674ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0061528440.9938472
ENST00000357674ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0034851190.9965148
ENST00000314062ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.0035093950.99649054
ENST00000314062ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0061528440.9938472
ENST00000314062ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0034851190.9965148
ENST00000346964ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.0035093950.99649054
ENST00000346964ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0061528440.9938472
ENST00000346964ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0034851190.9965148
ENST00000419354ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.0035248690.99647516
ENST00000419354ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0062449330.993755
ENST00000419354ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0035008560.9964992
ENST00000422288ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.0036227830.99637717
ENST00000422288ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0062249370.993775
ENST00000422288ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0035981370.9964019
ENST00000448528ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.0035093950.99649054
ENST00000448528ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0061528440.9938472
ENST00000448528ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0034851190.9965148
ENST00000450955ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.0036227830.99637717
ENST00000450955ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0062249370.993775
ENST00000450955ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0035981370.9964019
ENST00000392382ENST00000595618DLG1chr3196921296-MYO9Bchr1917301906+0.003487290.9965127
ENST00000392382ENST00000594824DLG1chr3196921296-MYO9Bchr1917301906+0.0060640160.993936
ENST00000392382ENST00000397274DLG1chr3196921296-MYO9Bchr1917301906+0.0034627720.9965372
ENST00000357674ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.0035093950.99649054
ENST00000357674ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0061528440.9938472
ENST00000357674ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0034851190.9965148
ENST00000314062ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.0035093950.99649054
ENST00000314062ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0061528440.9938472
ENST00000314062ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0034851190.9965148
ENST00000346964ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.0035093950.99649054
ENST00000346964ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0061528440.9938472
ENST00000346964ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0034851190.9965148
ENST00000419354ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.0035248690.99647516
ENST00000419354ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0062449330.993755
ENST00000419354ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0035008560.9964992
ENST00000422288ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.0036227830.99637717
ENST00000422288ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0062249370.993775
ENST00000422288ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0035981370.9964019
ENST00000448528ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.0035093950.99649054
ENST00000448528ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0061528440.9938472
ENST00000448528ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0034851190.9965148
ENST00000450955ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.0036227830.99637717
ENST00000450955ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0062249370.993775
ENST00000450955ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0035981370.9964019
ENST00000392382ENST00000595618DLG1chr3196921295-MYO9Bchr1917301905+0.003487290.9965127
ENST00000392382ENST00000594824DLG1chr3196921295-MYO9Bchr1917301905+0.0060640160.993936
ENST00000392382ENST00000397274DLG1chr3196921295-MYO9Bchr1917301905+0.0034627720.9965372

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for DLG1-MYO9B

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
DLG1chr3196921295MYO9Bchr1917301905483161HGFVSHSHISPIKDFTEQFQVLLPKD
DLG1chr3196921295MYO9Bchr1917301905557161HGFVSHSHISPIKDFTEQFQVLLPKD
DLG1chr3196921295MYO9Bchr1917301905673161HGFVSHSHISPIKDFTEQFQVLLPKD
DLG1chr3196921295MYO9Bchr1917301905770161HGFVSHSHISPIKDFTEQFQVLLPKD
DLG1chr3196921296MYO9Bchr1917301906483161HGFVSHSHISPIKDFTEQFQVLLPKD
DLG1chr3196921296MYO9Bchr1917301906557161HGFVSHSHISPIKDFTEQFQVLLPKD
DLG1chr3196921296MYO9Bchr1917301906673161HGFVSHSHISPIKDFTEQFQVLLPKD
DLG1chr3196921296MYO9Bchr1917301906770161HGFVSHSHISPIKDFTEQFQVLLPKD

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Potential FusionNeoAntigen Information of DLG1-MYO9B in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
DLG1-MYO9B_196921295_17301905.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B39:01SHISPIKDF0.98640.7581615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:01SHISPIKDF0.96150.8448615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:02SHISPIKDF0.95980.8612615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B13:02KDFTEQFQV0.940.89591221
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B13:01KDFTEQFQV0.77760.97091221
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B14:02SHISPIKDF0.75740.5023615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B14:01SHISPIKDF0.75740.5023615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:18SHISPIKDF0.71860.5577615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:03SHISPIKDF0.65340.6331615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B47:01KDFTEQFQV0.38240.75021221
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:01SPIKDFTEQF0.99340.7841919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:08SPIKDFTEQF0.99060.7957919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:18HSHISPIKDF0.96190.5298515
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:03SPIKDFTEQF0.94450.8608919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:02SPIKDFTEQF0.8070.9123919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:04SPIKDFTEQF0.8070.9123919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:01HSHISPIKDF0.51170.8015515
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:02HSHISPIKDF0.46260.8136515
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B57:03ISPIKDFTEQF0.99980.9577819
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:01SHSHISPIKDF0.99580.8263415
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:02SHSHISPIKDF0.99420.837415
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B39:12SHISPIKDF0.98760.7694615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B39:05SHISPIKDF0.96560.738615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:95SHISPIKDF0.81530.5503615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:05SHISPIKDF0.80830.93615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:27SHISPIKDF0.80220.9306615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:29SHISPIKDF0.74480.9163615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:13SHISPIKDF0.73980.8821615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:67SHISPIKDF0.63910.9218615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:80SHISPIKDF0.63910.9218615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:10SHISPIKDF0.63780.9484615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:19SHISPIKDF0.63290.6275615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:46SHISPIKDF0.61450.8341615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:21SHISPIKDF0.44880.8328615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C12:16SHISPIKDF0.02150.9399615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B14:03SHISPIKDF0.00730.5418615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:31SPIKDFTEQF0.99120.759919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B42:02SPIKDFTEQF0.9080.6373919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B42:01SPIKDFTEQF0.85980.6324919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:12SPIKDFTEQF0.8070.9123919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B39:10SPIKDFTEQF0.71010.908919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B39:02SHISPIKDF0.98940.7237615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B39:31SHISPIKDF0.98480.7634615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:05SHISPIKDF0.96150.8448615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:01SHISPIKDF0.80390.5258615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B48:02SHISPIKDF0.71680.8601615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:17SHISPIKDF0.70080.9539615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:22SHISPIKDF0.68760.5496615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C07:02SHISPIKDF0.63910.9218615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C03:67SHISPIKDF0.58010.9646615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C06:06SHISPIKDF0.06690.9681615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-C06:08SHISPIKDF0.04890.9793615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:54SHISPIKDF0.01990.8428615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:68SHISPIKDF0.0180.5007615
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:77SPIKDFTEQF0.99340.7841919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:23SPIKDFTEQF0.99240.8186919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:20SPIKDFTEQF0.99070.8261919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:30SPIKDFTEQF0.98560.6203919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:17SPIKDFTEQF0.98560.6203919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:43SPIKDFTEQF0.97960.797919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:08SPIKDFTEQF0.97930.8035919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:11SPIKDFTEQF0.97710.8019919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:11SPIKDFTEQF0.97440.8299919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:13SPIKDFTEQF0.94260.6002919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B15:12SPIKDFTEQF0.94040.8504919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:24SPIKDFTEQF0.90840.8804919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B35:09SPIKDFTEQF0.8070.9123919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B67:01SPIKDFTEQF0.73320.7704919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:05HSHISPIKDF0.51170.8015515
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B18:07SPIKDFTEQF0.2460.9032919
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B57:02ISPIKDFTEQF0.99960.8117819
DLG1-MYO9Bchr3196921295chr1917301905673HLA-B38:05SHSHISPIKDF0.99580.8263415

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Potential FusionNeoAntigen Information of DLG1-MYO9B in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
DLG1-MYO9B_196921295_17301905.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
DLG1-MYO9Bchr3196921295chr1917301905673DRB1-0411HSHISPIKDFTEQFQ520
DLG1-MYO9Bchr3196921295chr1917301905673DRB1-0411SHSHISPIKDFTEQF419
DLG1-MYO9Bchr3196921295chr1917301905673DRB1-0459HSHISPIKDFTEQFQ520
DLG1-MYO9Bchr3196921295chr1917301905673DRB1-0467HSHISPIKDFTEQFQ520
DLG1-MYO9Bchr3196921295chr1917301905673DRB1-0491HSHISPIKDFTEQFQ520
DLG1-MYO9Bchr3196921295chr1917301905673DRB1-0491SHSHISPIKDFTEQF419
DLG1-MYO9Bchr3196921295chr1917301905673DRB1-1457HSHISPIKDFTEQFQ520

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Fusion breakpoint peptide structures of DLG1-MYO9B

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
8621SHISPIKDFTEQFQDLG1MYO9Bchr3196921295chr1917301905673

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of DLG1-MYO9B

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN8621SHISPIKDFTEQFQ-7.9962-8.1096
HLA-B14:023BVN8621SHISPIKDFTEQFQ-5.70842-6.74372
HLA-B52:013W398621SHISPIKDFTEQFQ-6.83737-6.95077
HLA-B52:013W398621SHISPIKDFTEQFQ-4.4836-5.5189
HLA-A11:014UQ28621SHISPIKDFTEQFQ-10.0067-10.1201
HLA-A11:014UQ28621SHISPIKDFTEQFQ-9.03915-10.0745
HLA-A24:025HGA8621SHISPIKDFTEQFQ-6.56204-6.67544
HLA-A24:025HGA8621SHISPIKDFTEQFQ-5.42271-6.45801
HLA-B44:053DX88621SHISPIKDFTEQFQ-7.85648-8.89178
HLA-B44:053DX88621SHISPIKDFTEQFQ-5.3978-5.5112
HLA-A02:016TDR8621SHISPIKDFTEQFQ-3.37154-4.40684

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Vaccine Design for the FusionNeoAntigens of DLG1-MYO9B

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
DLG1-MYO9Bchr3196921295chr19173019051221KDFTEQFQVAAGGATTTCACCGAGCAGTTCCAGGTG
DLG1-MYO9Bchr3196921295chr1917301905415SHSHISPIKDFTCTCATTCTCATATTTCACCAATAAAGGATTTC
DLG1-MYO9Bchr3196921295chr1917301905515HSHISPIKDFCATTCTCATATTTCACCAATAAAGGATTTC
DLG1-MYO9Bchr3196921295chr1917301905615SHISPIKDFTCTCATATTTCACCAATAAAGGATTTC
DLG1-MYO9Bchr3196921295chr1917301905819ISPIKDFTEQFATTTCACCAATAAAGGATTTCACCGAGCAGTTC
DLG1-MYO9Bchr3196921295chr1917301905919SPIKDFTEQFTCACCAATAAAGGATTTCACCGAGCAGTTC

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
DLG1-MYO9Bchr3196921295chr1917301905419SHSHISPIKDFTEQFTCTCATTCTCATATTTCACCAATAAAGGATTTCACCGAGCAGTTC
DLG1-MYO9Bchr3196921295chr1917301905520HSHISPIKDFTEQFQCATTCTCATATTTCACCAATAAAGGATTTCACCGAGCAGTTCCAG

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Information of the samples that have these potential fusion neoantigens of DLG1-MYO9B

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BLCADLG1-MYO9Bchr3196921295ENST00000314062chr1917301905ENST00000397274TCGA-FD-A6TG

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Potential target of CAR-T therapy development for DLG1-MYO9B

check button Predicted 3D structure. We used RoseTTAFold.
138_DLG1-MYO9B_t000_.e2e.pdb


check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result
DLG1chr3196921295ENST00000314062MYO9Bchr1917301905ENST00000397274
DLG1chr3196921295ENST00000314062MYO9Bchr1917301905ENST00000594824

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Related Drugs to DLG1-MYO9B

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to DLG1-MYO9B

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource