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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:EIF4H-GTF2I

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: EIF4H-GTF2I
FusionPDB ID: 26066
FusionGDB2.0 ID: 26066
HgeneTgene
Gene symbol

EIF4H

GTF2I

Gene ID

7458

2969

Gene nameeukaryotic translation initiation factor 4Hgeneral transcription factor IIi
SynonymsWBSCR1|WSCR1|eIF-4HBAP135|BTKAP1|DIWS|GTFII-I|IB291|SPIN|TFII-I|WBS|WBSCR6
Cytomap

7q11.23

7q11.23

Type of geneprotein-codingprotein-coding
Descriptioneukaryotic translation initiation factor 4HWilliams-Beuren syndrome chromosome region 1general transcription factor II-IBTK-associated protein, 135kDBruton tyrosine kinase-associated protein 135SRF-Phox1-interacting proteinWilliams-Beuren syndrome chromosome region 6
Modification date2020031320200313
UniProtAcc

Q15056

Main function of 5'-partner protein: FUNCTION: Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA. {ECO:0000269|PubMed:10585411, ECO:0000269|PubMed:11418588}.

Q6EKJ0

Main function of 5'-partner protein:
Ensembl transtripts involved in fusion geneENST idsENST00000495187, ENST00000265753, 
ENST00000353999, 		ENST00000324896, 
ENST00000346152, ENST00000353920, 
ENST00000416070, ENST00000438130, 
ENST00000443166, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score5 X 8 X 4=16021 X 20 X 13=5460
# samples 725
** MAII scorelog2(7/160*10)=-1.1926450779424
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(25/5460*10)=-4.44890095114513
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: EIF4H [Title/Abstract] AND GTF2I [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: EIF4H [Title/Abstract] AND GTF2I [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)EIF4H(73604248)-GTF2I(74173109), # samples:1
EIF4H(73604636)-GTF2I(74173109), # samples:1
EIF4H(73604636)-GTF2I(74173110), # samples:1
GTF2I(74131270)-EIF4H(73604003), # samples:1
Anticipated loss of major functional domain due to fusion event.EIF4H-GTF2I seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
EIF4H-GTF2I seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
EIF4H-GTF2I seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
EIF4H-GTF2I seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
GTF2I-EIF4H seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
GTF2I-EIF4H seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
GTF2I-EIF4H seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF.
GTF2I-EIF4H seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
GTF2I-EIF4H seems lost the major protein functional domain in Hgene partner, which is a transcription factor due to the frame-shifted ORF.
GTF2I-EIF4H seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
GTF2I-EIF4H seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneGTF2I

GO:0016525

negative regulation of angiogenesis

19242469



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:73604248/chr7:74173109)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across EIF4H (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across GTF2I (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000265753EIF4Hchr773604248+ENST00000324896GTF2Ichr774173109+1769548139606155
ENST00000265753EIF4Hchr773604248+ENST00000353920GTF2Ichr774173109+1769548139606155
ENST00000265753EIF4Hchr773604248+ENST00000346152GTF2Ichr774173109+1769548139606155
ENST00000265753EIF4Hchr773604248+ENST00000416070GTF2Ichr774173109+1773548139606155
ENST00000353999EIF4Hchr773604248+ENST00000324896GTF2Ichr774173109+165843728495155
ENST00000353999EIF4Hchr773604248+ENST00000353920GTF2Ichr774173109+165843728495155
ENST00000353999EIF4Hchr773604248+ENST00000346152GTF2Ichr774173109+165843728495155
ENST00000353999EIF4Hchr773604248+ENST00000416070GTF2Ichr774173109+166243728495155
ENST00000265753EIF4Hchr773604636+ENST00000324896GTF2Ichr774173109+1829608139666175
ENST00000265753EIF4Hchr773604636+ENST00000353920GTF2Ichr774173109+1829608139666175
ENST00000265753EIF4Hchr773604636+ENST00000346152GTF2Ichr774173109+1829608139666175
ENST00000265753EIF4Hchr773604636+ENST00000416070GTF2Ichr774173109+1833608139666175
ENST00000265753EIF4Hchr773604636+ENST00000324896GTF2Ichr774173110+1829608139666175
ENST00000265753EIF4Hchr773604636+ENST00000353920GTF2Ichr774173110+1829608139666175
ENST00000265753EIF4Hchr773604636+ENST00000346152GTF2Ichr774173110+1829608139666175
ENST00000265753EIF4Hchr773604636+ENST00000416070GTF2Ichr774173110+1833608139666175

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000265753ENST00000324896EIF4Hchr773604248+GTF2Ichr774173109+0.0041427130.9958573
ENST00000265753ENST00000353920EIF4Hchr773604248+GTF2Ichr774173109+0.0041427130.9958573
ENST00000265753ENST00000346152EIF4Hchr773604248+GTF2Ichr774173109+0.0041427130.9958573
ENST00000265753ENST00000416070EIF4Hchr773604248+GTF2Ichr774173109+0.0044434850.99555653
ENST00000353999ENST00000324896EIF4Hchr773604248+GTF2Ichr774173109+0.0030107110.9969893
ENST00000353999ENST00000353920EIF4Hchr773604248+GTF2Ichr774173109+0.0030107110.9969893
ENST00000353999ENST00000346152EIF4Hchr773604248+GTF2Ichr774173109+0.0030107110.9969893
ENST00000353999ENST00000416070EIF4Hchr773604248+GTF2Ichr774173109+0.0030225080.99697745
ENST00000265753ENST00000324896EIF4Hchr773604636+GTF2Ichr774173109+0.001553370.99844664
ENST00000265753ENST00000353920EIF4Hchr773604636+GTF2Ichr774173109+0.001553370.99844664
ENST00000265753ENST00000346152EIF4Hchr773604636+GTF2Ichr774173109+0.001553370.99844664
ENST00000265753ENST00000416070EIF4Hchr773604636+GTF2Ichr774173109+0.0015454910.99845445
ENST00000265753ENST00000324896EIF4Hchr773604636+GTF2Ichr774173110+0.001553370.99844664
ENST00000265753ENST00000353920EIF4Hchr773604636+GTF2Ichr774173110+0.001553370.99844664
ENST00000265753ENST00000346152EIF4Hchr773604636+GTF2Ichr774173110+0.001553370.99844664
ENST00000265753ENST00000416070EIF4Hchr773604636+GTF2Ichr774173110+0.0015454910.99845445

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for EIF4H-GTF2I

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
EIF4Hchr773604248GTF2Ichr774173109437136GGFGFRKGGPDDREIKETDGSSQIKQ
EIF4Hchr773604248GTF2Ichr774173109548136GGFGFRKGGPDDREIKETDGSSQIKQ
EIF4Hchr773604636GTF2Ichr774173109608156SRGGWDSRDDFNSEIKETDGSSQIKQ
EIF4Hchr773604636GTF2Ichr774173110608156SRGGWDSRDDFNSEIKETDGSSQIKQ

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Potential FusionNeoAntigen Information of EIF4H-GTF2I in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
EIF4H-GTF2I_73604636_74173109.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
EIF4H-GTF2Ichr773604636chr774173109608HLA-B39:01SRDDFNSEI0.9970.5611615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B38:02SRDDFNSEI0.99590.7059615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B38:01SRDDFNSEI0.99440.7042615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C04:10SRDDFNSEI0.99660.6189615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B39:12SRDDFNSEI0.99580.5731615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C04:07SRDDFNSEI0.99540.6195615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B39:05SRDDFNSEI0.99410.5447615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:13SRDDFNSEI0.9720.773615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:05SRDDFNSEI0.96980.8849615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:27SRDDFNSEI0.96790.8951615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:29SRDDFNSEI0.96720.8021615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:10SRDDFNSEI0.87250.8434615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:46SRDDFNSEI0.85750.6474615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:80SRDDFNSEI0.83530.8263615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:67SRDDFNSEI0.83530.8263615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C04:14SRDDFNSEI0.48520.605615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C12:16SRDDFNSEI0.01680.8743615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B39:31SRDDFNSEI0.99760.5624615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C18:01SRDDFNSEI0.9960.7054615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C04:01SRDDFNSEI0.99540.6195615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B38:05SRDDFNSEI0.99440.7042615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B27:06SRDDFNSEI0.98780.5199615
EIF4H-GTF2Ichr773604636chr774173109608HLA-B27:09SRDDFNSEI0.98680.683615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:17SRDDFNSEI0.94840.8834615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:02SRDDFNSEI0.83530.8263615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C06:06SRDDFNSEI0.83050.9557615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C07:04SRDDFNSEI0.77020.828615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C06:08SRDDFNSEI0.72440.9443615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C04:04SRDDFNSEI0.61850.6225615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C06:02SRDDFNSEI0.02330.9646615
EIF4H-GTF2Ichr773604636chr774173109608HLA-C06:17SRDDFNSEI0.02330.9646615

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Potential FusionNeoAntigen Information of EIF4H-GTF2I in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
EIF4H-GTF2I_73604636_74173109.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0466RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0801RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0801SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0801DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0803RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0803SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0805RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0805SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0805DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0805DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0808RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0808SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0811RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0811SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0814RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0814SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0816RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0816SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0816DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0818RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0818SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0818DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0823RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0823SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0826RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0826SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0826DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0827RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0827SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0829RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0829SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0829DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0832RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0833RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0833SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0835RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0835SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0836RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0836SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0837RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0838RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0838SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0839RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0839SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0839DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0840RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0840SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0840DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-0840DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1153RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1179RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1303RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1303SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1303DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1303DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-13101RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-13101SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-13101DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-13101DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1310RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1310SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1310DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1310DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1312RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1312SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1312DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1312DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1313RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1313SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1313DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1321RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1321SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1321DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1330RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1330SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1330DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1333RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1333SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1333DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1333DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1337RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1338RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1338SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1349RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1349SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1349DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1349DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1355RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1355SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1355DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1365RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1365SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1366RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1366SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1366DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1366DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1388RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1388SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1388DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1388DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1390RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1390SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1390DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1390DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1395RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1395SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1395DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1395DDFNSEIKETDGSSQ823
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1409RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1413RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1413SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1413DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1422RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1422SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1422DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1425RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1425SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1425DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1453RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1453SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1463RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1469RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1469SRDDFNSEIKETDGS621
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1469DSRDDFNSEIKETDG520
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1485RDDFNSEIKETDGSS722
EIF4H-GTF2Ichr773604636chr774173109608DRB1-1485SRDDFNSEIKETDGS621

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Fusion breakpoint peptide structures of EIF4H-GTF2I

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
8948SRDDFNSEIKETDGEIF4HGTF2Ichr773604636chr774173109608

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of EIF4H-GTF2I

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN8948SRDDFNSEIKETDG-7.15543-7.26883
HLA-B14:023BVN8948SRDDFNSEIKETDG-4.77435-5.80965
HLA-B52:013W398948SRDDFNSEIKETDG-6.80875-6.92215
HLA-B52:013W398948SRDDFNSEIKETDG-4.20386-5.23916
HLA-A11:014UQ28948SRDDFNSEIKETDG-7.5194-8.5547
HLA-A11:014UQ28948SRDDFNSEIKETDG-6.9601-7.0735
HLA-A24:025HGA8948SRDDFNSEIKETDG-7.52403-7.63743
HLA-A24:025HGA8948SRDDFNSEIKETDG-5.82433-6.85963
HLA-B27:056PYJ8948SRDDFNSEIKETDG-3.28285-4.31815
HLA-B44:053DX88948SRDDFNSEIKETDG-5.91172-6.94702
HLA-B44:053DX88948SRDDFNSEIKETDG-4.24346-4.35686

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Vaccine Design for the FusionNeoAntigens of EIF4H-GTF2I

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
EIF4H-GTF2Ichr773604636chr774173109615SRDDFNSEICCCGGGATGACTTCAATTCTGAAATAA

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
EIF4H-GTF2Ichr773604636chr774173109520DSRDDFNSEIKETDGATTCCCGGGATGACTTCAATTCTGAAATAAAAGAGACTGATGGAA
EIF4H-GTF2Ichr773604636chr774173109621SRDDFNSEIKETDGSCCCGGGATGACTTCAATTCTGAAATAAAAGAGACTGATGGAAGCT
EIF4H-GTF2Ichr773604636chr774173109722RDDFNSEIKETDGSSGGGATGACTTCAATTCTGAAATAAAAGAGACTGATGGAAGCTCTC
EIF4H-GTF2Ichr773604636chr774173109823DDFNSEIKETDGSSQATGACTTCAATTCTGAAATAAAAGAGACTGATGGAAGCTCTCAGA

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Information of the samples that have these potential fusion neoantigens of EIF4H-GTF2I

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
GBMEIF4H-GTF2Ichr773604636ENST00000265753chr774173109ENST00000324896TCGA-06-2557

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Potential target of CAR-T therapy development for EIF4H-GTF2I

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to EIF4H-GTF2I

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to EIF4H-GTF2I

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource