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Fusion Protein:ERCC1-ANKIB1 |
Fusion Gene and Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: ERCC1-ANKIB1 | FusionPDB ID: 27300 | FusionGDB2.0 ID: 27300 | Hgene | Tgene | Gene symbol | ERCC1 | ANKIB1 | Gene ID | 2067 | 54467 |
Gene name | ERCC excision repair 1, endonuclease non-catalytic subunit | ankyrin repeat and IBR domain containing 1 | |
Synonyms | COFS4|RAD10|UV20 | - | |
Cytomap | 19q13.32 | 7q21.2 | |
Type of gene | protein-coding | protein-coding | |
Description | DNA excision repair protein ERCC-1excision repair cross-complementation group 1excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) | ankyrin repeat and IBR domain-containing protein 1 | |
Modification date | 20200329 | 20200313 | |
UniProtAcc | P07992 Main function of 5'-partner protein: FUNCTION: [Isoform 1]: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. {ECO:0000269|PubMed:17273966, ECO:0000269|PubMed:23623389, ECO:0000269|PubMed:24036546}.; FUNCTION: [Isoform 2]: Not functional in the nucleotide excision repair pathway. {ECO:0000305|PubMed:24036546}.; FUNCTION: [Isoform 3]: Not functional in the nucleotide excision repair pathway. {ECO:0000305|PubMed:24036546}.; FUNCTION: [Isoform 4]: Not functional in the nucleotide excision repair pathway. {ECO:0000305|PubMed:24036546}. | Q9P2G1 Main function of 5'-partner protein: FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. {ECO:0000250}. | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000300853, ENST00000340192, ENST00000423698, ENST00000589165, ENST00000588738, ENST00000013807, ENST00000591636, | ENST00000486698, ENST00000265742, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 7 X 8 X 3=168 | 13 X 10 X 8=1040 |
# samples | 8 | 20 | |
** MAII score | log2(8/168*10)=-1.0703893278914 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(20/1040*10)=-2.37851162325373 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Fusion gene context | PubMed: ERCC1 [Title/Abstract] AND ANKIB1 [Title/Abstract] AND fusion [Title/Abstract] | ||
Fusion neoantigen context | PubMed: ERCC1 [Title/Abstract] AND ANKIB1 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | ERCC1(45916934)-ANKIB1(91991498), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. | ERCC1-ANKIB1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ERCC1-ANKIB1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ERCC1-ANKIB1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ERCC1-ANKIB1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ERCC1-ANKIB1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | ERCC1 | GO:0006289 | nucleotide-excision repair | 3290851 |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:45916934/chr7:91991498) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000423698 | ERCC1 | chr19 | 45916934 | - | ENST00000265742 | ANKIB1 | chr7 | 91991498 | + | 5035 | 727 | 653 | 2599 | 648 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000423698 | ENST00000265742 | ERCC1 | chr19 | 45916934 | - | ANKIB1 | chr7 | 91991498 | + | 0.000453536 | 0.99954647 |
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Get the fusion protein sequences from here. |
Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for ERCC1-ANKIB1 |
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Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
ERCC1 | chr19 | 45916934 | ANKIB1 | chr7 | 91991498 | 727 | 25 | KIWPYAQAWALRKECLGEAHEPCDCQ |
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Potential FusionNeoAntigen Information of ERCC1-ANKIB1 in HLA I |
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ERCC1-ANKIB1_45916934_91991498.msa |
![]() * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
ERCC1-ANKIB1 | chr19 | 45916934 | chr7 | 91991498 | 727 | HLA-B45:01 | KECLGEAHEP | 0.9255 | 0.8066 | 12 | 22 |
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Potential FusionNeoAntigen Information of ERCC1-ANKIB1 in HLA II |
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![]() * We used NetMHCIIpan v4.1 (%rank<0.5). |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of ERCC1-ANKIB1 |
![]() * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
7163 | QAWALRKECLGEAH | ERCC1 | ANKIB1 | chr19 | 45916934 | chr7 | 91991498 | 727 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ERCC1-ANKIB1 |
![]() * We used Glide to predict the interaction between HLAs and neoantigens. |
HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
HLA-B14:02 | 3BVN | 7163 | QAWALRKECLGEAH | -7.15543 | -7.26883 |
HLA-B14:02 | 3BVN | 7163 | QAWALRKECLGEAH | -4.77435 | -5.80965 |
HLA-B52:01 | 3W39 | 7163 | QAWALRKECLGEAH | -6.80875 | -6.92215 |
HLA-B52:01 | 3W39 | 7163 | QAWALRKECLGEAH | -4.20386 | -5.23916 |
HLA-A11:01 | 4UQ2 | 7163 | QAWALRKECLGEAH | -7.5194 | -8.5547 |
HLA-A11:01 | 4UQ2 | 7163 | QAWALRKECLGEAH | -6.9601 | -7.0735 |
HLA-A24:02 | 5HGA | 7163 | QAWALRKECLGEAH | -7.52403 | -7.63743 |
HLA-A24:02 | 5HGA | 7163 | QAWALRKECLGEAH | -5.82433 | -6.85963 |
HLA-B27:05 | 6PYJ | 7163 | QAWALRKECLGEAH | -3.28285 | -4.31815 |
HLA-B44:05 | 3DX8 | 7163 | QAWALRKECLGEAH | -5.91172 | -6.94702 |
HLA-B44:05 | 3DX8 | 7163 | QAWALRKECLGEAH | -4.24346 | -4.35686 |
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Vaccine Design for the FusionNeoAntigens of ERCC1-ANKIB1 |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
ERCC1-ANKIB1 | chr19 | 45916934 | chr7 | 91991498 | 12 | 22 | KECLGEAHEP | AAAGGAGTGCCTTGGTGAAGCACATGAGCC |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of ERCC1-ANKIB1 |
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Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
STAD | ERCC1-ANKIB1 | chr19 | 45916934 | ENST00000423698 | chr7 | 91991498 | ENST00000265742 | TCGA-R5-A7ZF |
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Potential target of CAR-T therapy development for ERCC1-ANKIB1 |
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![]() * Minus value of BPloci means that the break point is located before the CDS. |
- In-frame and retained 'Transmembrane'. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
![]() * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to ERCC1-ANKIB1 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to ERCC1-ANKIB1 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |