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Fusion Protein:FNIP1-CYP3A4 |
Fusion Gene and Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: FNIP1-CYP3A4 | FusionPDB ID: 30948 | FusionGDB2.0 ID: 30948 | Hgene | Tgene | Gene symbol | FNIP1 | CYP3A4 | Gene ID | 96459 | 1576 |
Gene name | folliculin interacting protein 1 | cytochrome P450 family 3 subfamily A member 4 | |
Synonyms | - | CP33|CP34|CYP3A|CYP3A3|CYPIIIA3|CYPIIIA4|HLP|NF-25|P450C3|P450PCN1 | |
Cytomap | 5q31.1 | 7q22.1 | |
Type of gene | protein-coding | protein-coding | |
Description | folliculin-interacting protein 1 | cytochrome P450 3A41,4-cineole 2-exo-monooxygenase1,8-cineole 2-exo-monooxygenaseP450-III, steroid induciblealbendazole monooxygenasealbendazole monooxygenase (sulfoxide-forming)albendazole sulfoxidasecholesterol 25-hydroxylasecytochrome P450 3A3 | |
Modification date | 20200327 | 20200327 | |
UniProtAcc | Q8TF40 Main function of 5'-partner protein: FUNCTION: Binding partner of the GTPase-activating protein FLCN: involved in the cellular response to amino acid availability by regulating the mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3 (PubMed:17028174, PubMed:18663353, PubMed:24081491). In low-amino acid conditions, component of the lysosomal folliculin complex (LFC) on the membrane of lysosomes, which inhibits the GTPase-activating activity of FLCN, thereby inactivating mTORC1 and promoting nuclear translocation of TFEB and TFE3 (By similarity). Upon amino acid restimulation, disassembly of the LFC complex liberates the GTPase-activating activity of FLCN, leading to activation of mTORC1 and subsequent cytoplasmic retention of TFEB and TFE3 (By similarity). Required to promote FLCN recruitment to lysosomes and interaction with Rag GTPases (PubMed:24081491). Together with FLCN, regulates autophagy: following phosphorylation by ULK1, interacts with GABARAP and promotes autophagy (PubMed:25126726). In addition to its role in mTORC1 signaling, also acts as a co-chaperone of HSP90AA1/Hsp90: following gradual phosphorylation by CK2, inhibits the ATPase activity of HSP90AA1/Hsp90, leading to activate both kinase and non-kinase client proteins of HSP90AA1/Hsp90 (PubMed:27353360, PubMed:30699359). Acts as a scaffold to load client protein FLCN onto HSP90AA1/Hsp90 (PubMed:27353360). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Required for B-cell development (By similarity). {ECO:0000250|UniProtKB:Q68FD7, ECO:0000250|UniProtKB:Q9P278, ECO:0000269|PubMed:17028174, ECO:0000269|PubMed:18663353, ECO:0000269|PubMed:24081491, ECO:0000269|PubMed:25126726, ECO:0000269|PubMed:27353360, ECO:0000269|PubMed:30699359}. | Q9HB55 Main function of 5'-partner protein: FUNCTION: Exhibits low testosterone 6-beta-hydroxylase activity. | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000307954, ENST00000307968, ENST00000510461, ENST00000511848, | ENST00000354593, ENST00000336411, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 15 X 11 X 10=1650 | 4 X 4 X 3=48 |
# samples | 18 | 4 | |
** MAII score | log2(18/1650*10)=-3.1963972128035 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(4/48*10)=-0.263034405833794 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Fusion gene context | PubMed: FNIP1 [Title/Abstract] AND CYP3A4 [Title/Abstract] AND fusion [Title/Abstract] | ||
Fusion neoantigen context | PubMed: FNIP1 [Title/Abstract] AND CYP3A4 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | FNIP1(131132523)-CYP3A4(99355851), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. | FNIP1-CYP3A4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FNIP1-CYP3A4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. FNIP1-CYP3A4 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. FNIP1-CYP3A4 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | FNIP1 | GO:0000122 | negative regulation of transcription by RNA polymerase II | 21209915 |
Hgene | FNIP1 | GO:0001932 | regulation of protein phosphorylation | 18663353 |
Hgene | FNIP1 | GO:0031334 | positive regulation of protein complex assembly | 25126726 |
Hgene | FNIP1 | GO:0033138 | positive regulation of peptidyl-serine phosphorylation | 19914239 |
Tgene | CYP3A4 | GO:0002933 | lipid hydroxylation | 14559847 |
Tgene | CYP3A4 | GO:0008202 | steroid metabolic process | 14559847 |
Tgene | CYP3A4 | GO:0008210 | estrogen metabolic process | 11555828|12865317|14559847 |
Tgene | CYP3A4 | GO:0009822 | alkaloid catabolic process | 15039299 |
Tgene | CYP3A4 | GO:0016098 | monoterpenoid metabolic process | 16401082 |
Tgene | CYP3A4 | GO:0017144 | drug metabolic process | 15327587|19219744 |
Tgene | CYP3A4 | GO:0042572 | retinol metabolic process | 10681376 |
Tgene | CYP3A4 | GO:0042573 | retinoic acid metabolic process | 11093772 |
Tgene | CYP3A4 | GO:0042737 | drug catabolic process | 15039299 |
Tgene | CYP3A4 | GO:0042738 | exogenous drug catabolic process | 18619574 |
Tgene | CYP3A4 | GO:0046483 | heterocycle metabolic process | 15327587 |
Tgene | CYP3A4 | GO:0055114 | oxidation-reduction process | 16401082|19219744 |
Tgene | CYP3A4 | GO:0070989 | oxidative demethylation | 15039299|18619574 |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:131132523/chr7:99355851) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000307968 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 645 | 92 | 161 | 0 | 54 |
ENST00000307954 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 674 | 121 | 190 | 2 | 63 |
ENST00000510461 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 741 | 188 | 257 | 51 | 68 |
ENST00000511848 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 740 | 187 | 256 | 50 | 68 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000307968 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9679813 | 0.032018688 |
ENST00000307954 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9764025 | 0.023597522 |
ENST00000510461 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9145837 | 0.085416354 |
ENST00000511848 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9312419 | 0.068758085 |
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Get the fusion protein sequences from here. |
Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for FNIP1-CYP3A4 |
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Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
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Potential FusionNeoAntigen Information of FNIP1-CYP3A4 in HLA I |
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![]() * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Potential FusionNeoAntigen Information of FNIP1-CYP3A4 in HLA II |
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![]() * We used NetMHCIIpan v4.1 (%rank<0.5). |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of FNIP1-CYP3A4 |
![]() * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of FNIP1-CYP3A4 |
![]() * We used Glide to predict the interaction between HLAs and neoantigens. |
HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
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Vaccine Design for the FusionNeoAntigens of FNIP1-CYP3A4 |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of FNIP1-CYP3A4 |
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Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
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Potential target of CAR-T therapy development for FNIP1-CYP3A4 |
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![]() * Minus value of BPloci means that the break point is located before the CDS. |
- In-frame and retained 'Transmembrane'. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
![]() * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to FNIP1-CYP3A4 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to FNIP1-CYP3A4 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |