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Fusion Protein:FNIP1-CYP3A4 |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: FNIP1-CYP3A4 | FusionPDB ID: 30948 | FusionGDB2.0 ID: 30948 | Hgene | Tgene | Gene symbol | FNIP1 | CYP3A4 | Gene ID | 96459 | 1576 |
| Gene name | folliculin interacting protein 1 | cytochrome P450 family 3 subfamily A member 4 | |
| Synonyms | - | CP33|CP34|CYP3A|CYP3A3|CYPIIIA3|CYPIIIA4|HLP|NF-25|P450C3|P450PCN1 | |
| Cytomap | 5q31.1 | 7q22.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | folliculin-interacting protein 1 | cytochrome P450 3A41,4-cineole 2-exo-monooxygenase1,8-cineole 2-exo-monooxygenaseP450-III, steroid induciblealbendazole monooxygenasealbendazole monooxygenase (sulfoxide-forming)albendazole sulfoxidasecholesterol 25-hydroxylasecytochrome P450 3A3 | |
| Modification date | 20200327 | 20200327 | |
| UniProtAcc | Q8TF40 Main function of 5'-partner protein: FUNCTION: Binding partner of the GTPase-activating protein FLCN: involved in the cellular response to amino acid availability by regulating the mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3 (PubMed:17028174, PubMed:18663353, PubMed:24081491). In low-amino acid conditions, component of the lysosomal folliculin complex (LFC) on the membrane of lysosomes, which inhibits the GTPase-activating activity of FLCN, thereby inactivating mTORC1 and promoting nuclear translocation of TFEB and TFE3 (By similarity). Upon amino acid restimulation, disassembly of the LFC complex liberates the GTPase-activating activity of FLCN, leading to activation of mTORC1 and subsequent cytoplasmic retention of TFEB and TFE3 (By similarity). Required to promote FLCN recruitment to lysosomes and interaction with Rag GTPases (PubMed:24081491). Together with FLCN, regulates autophagy: following phosphorylation by ULK1, interacts with GABARAP and promotes autophagy (PubMed:25126726). In addition to its role in mTORC1 signaling, also acts as a co-chaperone of HSP90AA1/Hsp90: following gradual phosphorylation by CK2, inhibits the ATPase activity of HSP90AA1/Hsp90, leading to activate both kinase and non-kinase client proteins of HSP90AA1/Hsp90 (PubMed:27353360, PubMed:30699359). Acts as a scaffold to load client protein FLCN onto HSP90AA1/Hsp90 (PubMed:27353360). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Required for B-cell development (By similarity). {ECO:0000250|UniProtKB:Q68FD7, ECO:0000250|UniProtKB:Q9P278, ECO:0000269|PubMed:17028174, ECO:0000269|PubMed:18663353, ECO:0000269|PubMed:24081491, ECO:0000269|PubMed:25126726, ECO:0000269|PubMed:27353360, ECO:0000269|PubMed:30699359}. | Q9HB55 Main function of 5'-partner protein: FUNCTION: Exhibits low testosterone 6-beta-hydroxylase activity. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000307954, ENST00000307968, ENST00000510461, ENST00000511848, | ENST00000354593, ENST00000336411, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 15 X 11 X 10=1650 | 4 X 4 X 3=48 |
| # samples | 18 | 4 | |
| ** MAII score | log2(18/1650*10)=-3.1963972128035 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(4/48*10)=-0.263034405833794 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: FNIP1 [Title/Abstract] AND CYP3A4 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: FNIP1 [Title/Abstract] AND CYP3A4 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | FNIP1(131132523)-CYP3A4(99355851), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | FNIP1-CYP3A4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FNIP1-CYP3A4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. FNIP1-CYP3A4 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. FNIP1-CYP3A4 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | FNIP1 | GO:0000122 | negative regulation of transcription by RNA polymerase II | 21209915 |
| Hgene | FNIP1 | GO:0001932 | regulation of protein phosphorylation | 18663353 |
| Hgene | FNIP1 | GO:0031334 | positive regulation of protein complex assembly | 25126726 |
| Hgene | FNIP1 | GO:0033138 | positive regulation of peptidyl-serine phosphorylation | 19914239 |
| Tgene | CYP3A4 | GO:0002933 | lipid hydroxylation | 14559847 |
| Tgene | CYP3A4 | GO:0008202 | steroid metabolic process | 14559847 |
| Tgene | CYP3A4 | GO:0008210 | estrogen metabolic process | 11555828|12865317|14559847 |
| Tgene | CYP3A4 | GO:0009822 | alkaloid catabolic process | 15039299 |
| Tgene | CYP3A4 | GO:0016098 | monoterpenoid metabolic process | 16401082 |
| Tgene | CYP3A4 | GO:0017144 | drug metabolic process | 15327587|19219744 |
| Tgene | CYP3A4 | GO:0042572 | retinol metabolic process | 10681376 |
| Tgene | CYP3A4 | GO:0042573 | retinoic acid metabolic process | 11093772 |
| Tgene | CYP3A4 | GO:0042737 | drug catabolic process | 15039299 |
| Tgene | CYP3A4 | GO:0042738 | exogenous drug catabolic process | 18619574 |
| Tgene | CYP3A4 | GO:0046483 | heterocycle metabolic process | 15327587 |
| Tgene | CYP3A4 | GO:0055114 | oxidation-reduction process | 16401082|19219744 |
| Tgene | CYP3A4 | GO:0070989 | oxidative demethylation | 15039299|18619574 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:131132523/chr7:99355851) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across FNIP1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across CYP3A4 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000307968 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 645 | 92 | 161 | 0 | 54 |
| ENST00000307954 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 674 | 121 | 190 | 2 | 63 |
| ENST00000510461 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 741 | 188 | 257 | 51 | 68 |
| ENST00000511848 | FNIP1 | chr5 | 131132523 | - | ENST00000336411 | CYP3A4 | chr7 | 99355851 | - | 740 | 187 | 256 | 50 | 68 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000307968 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9679813 | 0.032018688 |
| ENST00000307954 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9764025 | 0.023597522 |
| ENST00000510461 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9145837 | 0.085416354 |
| ENST00000511848 | ENST00000336411 | FNIP1 | chr5 | 131132523 | - | CYP3A4 | chr7 | 99355851 | - | 0.9312419 | 0.068758085 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for FNIP1-CYP3A4 |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
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Potential FusionNeoAntigen Information of FNIP1-CYP3A4 in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Potential FusionNeoAntigen Information of FNIP1-CYP3A4 in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of FNIP1-CYP3A4 |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of FNIP1-CYP3A4 |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
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Vaccine Design for the FusionNeoAntigens of FNIP1-CYP3A4 |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of FNIP1-CYP3A4 |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
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Potential target of CAR-T therapy development for FNIP1-CYP3A4 |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to FNIP1-CYP3A4 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to FNIP1-CYP3A4 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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