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Fusion Protein:FOXO1-UBQLN1 |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: FOXO1-UBQLN1 | FusionPDB ID: 31171 | FusionGDB2.0 ID: 31171 | Hgene | Tgene | Gene symbol | FOXO1 | UBQLN1 | Gene ID | 2308 | 29979 |
| Gene name | forkhead box O1 | ubiquilin 1 | |
| Synonyms | FKH1|FKHR|FOXO1A | DA41|DSK2|PLIC-1|UBQN|XDRP1 | |
| Cytomap | 13q14.11 | 9q21.32|9q21.2-q21.3 | |
| Type of gene | protein-coding | protein-coding | |
| Description | forkhead box protein O1forkhead box protein O1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma | ubiquilin-1hPLIC-1protein linking IAP with cytoskeleton 1testicular tissue protein Li 219 | |
| Modification date | 20200322 | 20200327 | |
| UniProtAcc | Q12778 Main function of 5'-partner protein: FUNCTION: Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed:10358076, PubMed:12228231, PubMed:15220471, PubMed:15890677, PubMed:18356527, PubMed:19221179, PubMed:20543840, PubMed:21245099). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed:10358076). Activity suppressed by insulin (PubMed:10358076). Main regulator of redox balance and osteoblast numbers and controls bone mass (By similarity). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (By similarity). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (By similarity). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (By similarity). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 (By similarity). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (PubMed:18356527, PubMed:19221179). Promotes neural cell death (PubMed:18356527). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (By similarity). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (PubMed:20543840). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity). Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed:31063815). {ECO:0000250|UniProtKB:A4L7N3, ECO:0000250|UniProtKB:G3V7R4, ECO:0000250|UniProtKB:Q9R1E0, ECO:0000269|PubMed:10358076, ECO:0000269|PubMed:12228231, ECO:0000269|PubMed:15220471, ECO:0000269|PubMed:15890677, ECO:0000269|PubMed:18356527, ECO:0000269|PubMed:19221179, ECO:0000269|PubMed:20543840, ECO:0000269|PubMed:21245099, ECO:0000269|PubMed:31063815}. | . | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000379561, ENST00000473775, | ENST00000257468, ENST00000376395, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 13 X 12 X 9=1404 | 15 X 10 X 9=1350 |
| # samples | 16 | 19 | |
| ** MAII score | log2(16/1404*10)=-3.1333991254172 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(19/1350*10)=-2.82888808360725 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: FOXO1 [Title/Abstract] AND UBQLN1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: FOXO1 [Title/Abstract] AND UBQLN1 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | FOXO1(41239719)-UBQLN1(86280060), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | FOXO1-UBQLN1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FOXO1-UBQLN1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FOXO1-UBQLN1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. FOXO1-UBQLN1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | FOXO1 | GO:0009267 | cellular response to starvation | 20543840 |
| Hgene | FOXO1 | GO:0032873 | negative regulation of stress-activated MAPK cascade | 19696738 |
| Hgene | FOXO1 | GO:0043066 | negative regulation of apoptotic process | 10871843 |
| Hgene | FOXO1 | GO:0045893 | positive regulation of transcription, DNA-templated | 7862145|10871843|12228231 |
| Hgene | FOXO1 | GO:0045944 | positive regulation of transcription by RNA polymerase II | 10871843|12228231 |
| Hgene | FOXO1 | GO:0071455 | cellular response to hyperoxia | 20543840 |
| Tgene | UBQLN1 | GO:0031396 | regulation of protein ubiquitination | 12634373 |
| Tgene | UBQLN1 | GO:0031398 | positive regulation of protein ubiquitination | 23307288 |
| Tgene | UBQLN1 | GO:0035973 | aggrephagy | 21143716 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr13:41239719/chr9:86280060) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across FOXO1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across UBQLN1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000379561 | FOXO1 | chr13 | 41239719 | - | ENST00000376395 | UBQLN1 | chr9 | 86280060 | - | 3277 | 1015 | 43 | 1452 | 469 |
| ENST00000379561 | FOXO1 | chr13 | 41239719 | - | ENST00000257468 | UBQLN1 | chr9 | 86280060 | - | 1811 | 1015 | 43 | 1452 | 469 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000379561 | ENST00000376395 | FOXO1 | chr13 | 41239719 | - | UBQLN1 | chr9 | 86280060 | - | 0.002854042 | 0.99714595 |
| ENST00000379561 | ENST00000257468 | FOXO1 | chr13 | 41239719 | - | UBQLN1 | chr9 | 86280060 | - | 0.03317468 | 0.96682537 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for FOXO1-UBQLN1 |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
| FOXO1 | chr13 | 41239719 | UBQLN1 | chr9 | 86280060 | 1015 | 324 | KDKGDSNSSAGWKMQNPDTLSAMSNP |
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Potential FusionNeoAntigen Information of FOXO1-UBQLN1 in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| FOXO1-UBQLN1_41239719_86280060.msa |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:01 | WKMQNPDTL | 0.9958 | 0.8562 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B38:02 | WKMQNPDTL | 0.9916 | 0.9528 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B38:01 | WKMQNPDTL | 0.9907 | 0.9496 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:13 | WKMQNPDTL | 0.9901 | 0.8806 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B14:02 | WKMQNPDTL | 0.8194 | 0.5795 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B14:01 | WKMQNPDTL | 0.8194 | 0.5795 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B15:18 | WKMQNPDTL | 0.4536 | 0.6474 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:09 | WKMQNPDTL | 0.9971 | 0.5712 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:12 | WKMQNPDTL | 0.9945 | 0.8717 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:05 | WKMQNPDTL | 0.9904 | 0.8479 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-C07:13 | WKMQNPDTL | 0.9309 | 0.9159 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:08 | WKMQNPDTL | 0.5648 | 0.8669 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B14:03 | WKMQNPDTL | 0.3559 | 0.6492 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:02 | WKMQNPDTL | 0.9957 | 0.8786 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:31 | WKMQNPDTL | 0.9953 | 0.8722 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B38:05 | WKMQNPDTL | 0.9907 | 0.9496 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B15:09 | WKMQNPDTL | 0.7552 | 0.7043 | 11 | 20 |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 | HLA-B39:11 | WKMQNPDTL | 0.5914 | 0.8254 | 11 | 20 |
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Potential FusionNeoAntigen Information of FOXO1-UBQLN1 in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of FOXO1-UBQLN1 |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
| File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
| 6391 | NSSAGWKMQNPDTL | FOXO1 | UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 1015 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of FOXO1-UBQLN1 |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
| HLA-B14:02 | 3BVN | 6391 | NSSAGWKMQNPDTL | -7.15543 | -7.26883 |
| HLA-B14:02 | 3BVN | 6391 | NSSAGWKMQNPDTL | -4.77435 | -5.80965 |
| HLA-B52:01 | 3W39 | 6391 | NSSAGWKMQNPDTL | -6.80875 | -6.92215 |
| HLA-B52:01 | 3W39 | 6391 | NSSAGWKMQNPDTL | -4.20386 | -5.23916 |
| HLA-A11:01 | 4UQ2 | 6391 | NSSAGWKMQNPDTL | -7.5194 | -8.5547 |
| HLA-A11:01 | 4UQ2 | 6391 | NSSAGWKMQNPDTL | -6.9601 | -7.0735 |
| HLA-A24:02 | 5HGA | 6391 | NSSAGWKMQNPDTL | -7.52403 | -7.63743 |
| HLA-A24:02 | 5HGA | 6391 | NSSAGWKMQNPDTL | -5.82433 | -6.85963 |
| HLA-B27:05 | 6PYJ | 6391 | NSSAGWKMQNPDTL | -3.28285 | -4.31815 |
| HLA-B44:05 | 3DX8 | 6391 | NSSAGWKMQNPDTL | -5.91172 | -6.94702 |
| HLA-B44:05 | 3DX8 | 6391 | NSSAGWKMQNPDTL | -4.24346 | -4.35686 |
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Vaccine Design for the FusionNeoAntigens of FOXO1-UBQLN1 |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| FOXO1-UBQLN1 | chr13 | 41239719 | chr9 | 86280060 | 11 | 20 | WKMQNPDTL | TGGAAGATGCAGAATCCTGATACACTA |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of FOXO1-UBQLN1 |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
| STAD | FOXO1-UBQLN1 | chr13 | 41239719 | ENST00000379561 | chr9 | 86280060 | ENST00000257468 | TCGA-BR-A453 |
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Potential target of CAR-T therapy development for FOXO1-UBQLN1 |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to FOXO1-UBQLN1 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to FOXO1-UBQLN1 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | FOXO1 | C0020542 | Pulmonary Hypertension | 1 | CTD_human |
| Hgene | FOXO1 | C0022578 | Keratoconus | 1 | CTD_human |
| Hgene | FOXO1 | C0023467 | Leukemia, Myelocytic, Acute | 1 | CTD_human |
| Hgene | FOXO1 | C0026998 | Acute Myeloid Leukemia, M1 | 1 | CTD_human |
| Hgene | FOXO1 | C0033578 | Prostatic Neoplasms | 1 | CTD_human |
| Hgene | FOXO1 | C0206655 | Alveolar rhabdomyosarcoma | 1 | CTD_human;GENOMICS_ENGLAND;ORPHANET |
| Hgene | FOXO1 | C0376358 | Malignant neoplasm of prostate | 1 | CTD_human |
| Hgene | FOXO1 | C1879321 | Acute Myeloid Leukemia (AML-M2) | 1 | CTD_human |
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