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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:HAUS4-CAT

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: HAUS4-CAT
FusionPDB ID: 35644
FusionGDB2.0 ID: 35644
HgeneTgene
Gene symbol

HAUS4

CAT

Gene ID

54930

10249

Gene nameHAUS augmin like complex subunit 4glycine-N-acyltransferase
SynonymsC14orf94ACGNAT|CAT|GAT
Cytomap

14q11.2

11q12.1

Type of geneprotein-codingprotein-coding
DescriptionHAUS augmin-like complex subunit 4homologous to Augmin subunitsglycine N-acyltransferaseAAcHRP-1(CLP)acyl-CoA:glycine N-acyltransferasearalkyl acyl-CoA N-acyltransferasearalkyl acyl-CoA:amino acid N-acyltransferasearalkyl-CoA N-acyltransferasebenzoyl-coenzyme A:glycine N-acyltransferaseepididymis secretory sp
Modification date2020031320200313
UniProtAcc

Q9H6D7

Main function of 5'-partner protein: FUNCTION: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. {ECO:0000269|PubMed:19369198, ECO:0000269|PubMed:19427217}.

Q6ZRH7

Main function of 5'-partner protein: FUNCTION: Probably involved in sperm cell hyperactivation via its association with CATSPER1. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. {ECO:0000250|UniProtKB:C6KI89}.
Ensembl transtripts involved in fusion geneENST idsENST00000206474, ENST00000342454, 
ENST00000347758, ENST00000397409, 
ENST00000541587, ENST00000555367, 
ENST00000555986, ENST00000490506, 
ENST00000554446, 
ENST00000534710, 
ENST00000241052, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score2 X 2 X 2=814 X 11 X 8=1232
# samples 218
** MAII scorelog2(2/8*10)=1.32192809488736log2(18/1232*10)=-2.77493344436523
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: HAUS4 [Title/Abstract] AND CAT [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: HAUS4 [Title/Abstract] AND CAT [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)HAUS4(23421537)-CAT(34482798), # samples:1
Anticipated loss of major functional domain due to fusion event.HAUS4-CAT seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
HAUS4-CAT seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
HAUS4-CAT seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
HAUS4-CAT seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneCAT

GO:0006544

glycine metabolic process

22475485

TgeneCAT

GO:1901787

benzoyl-CoA metabolic process

22475485



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr14:23421537/chr11:34482798)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across HAUS4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across CAT (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000347758HAUS4chr1423421537-ENST00000241052CATchr1134482798+19127552151282355
ENST00000555986HAUS4chr1423421537-ENST00000241052CATchr1134482798+19017442041271355

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000347758ENST00000241052HAUS4chr1423421537-CATchr1134482798+0.0025205290.9974795
ENST00000555986ENST00000241052HAUS4chr1423421537-CATchr1134482798+0.0023207230.9976793

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for HAUS4-CAT

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
HAUS4chr1423421537CATchr1134482798744180KIQDTNVTSEDKKGRLFAYPDTHRHR
HAUS4chr1423421537CATchr1134482798755180KIQDTNVTSEDKKGRLFAYPDTHRHR

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Potential FusionNeoAntigen Information of HAUS4-CAT in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
HAUS4-CAT_23421537_34482798.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
HAUS4-CATchr1423421537chr1134482798755HLA-B44:03SEDKKGRLF0.99760.737817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:01SEDKKGRLF0.90460.6226817
HAUS4-CATchr1423421537chr1134482798755HLA-B44:03TSEDKKGRLF0.80250.7734717
HAUS4-CATchr1423421537chr1134482798755HLA-B57:01VTSEDKKGRLF0.99990.8683617
HAUS4-CATchr1423421537chr1134482798755HLA-B44:03SEDKKGRLFAY0.99970.7573819
HAUS4-CATchr1423421537chr1134482798755HLA-B58:02VTSEDKKGRLF0.99960.7536617
HAUS4-CATchr1423421537chr1134482798755HLA-B57:03VTSEDKKGRLF0.99920.8645617
HAUS4-CATchr1423421537chr1134482798755HLA-B44:13SEDKKGRLF0.99760.737817
HAUS4-CATchr1423421537chr1134482798755HLA-B44:07SEDKKGRLF0.99760.737817
HAUS4-CATchr1423421537chr1134482798755HLA-B44:26SEDKKGRLF0.99760.737817
HAUS4-CATchr1423421537chr1134482798755HLA-C18:01SEDKKGRLF0.99130.578817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:04SEDKKGRLF0.92110.6575817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:11SEDKKGRLF0.91090.5733817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:05SEDKKGRLF0.90460.6226817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:03SEDKKGRLF0.89280.6221817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:06SEDKKGRLF0.88820.5843817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:04DKKGRLFAY0.47210.78951019
HAUS4-CATchr1423421537chr1134482798755HLA-B48:02SEDKKGRLF0.40760.5768817
HAUS4-CATchr1423421537chr1134482798755HLA-B18:07DKKGRLFAY0.27020.69731019
HAUS4-CATchr1423421537chr1134482798755HLA-B18:03DKKGRLFAY0.26730.74571019
HAUS4-CATchr1423421537chr1134482798755HLA-B18:06DKKGRLFAY0.25870.7461019
HAUS4-CATchr1423421537chr1134482798755HLA-B15:53SEDKKGRLF0.06830.5412817
HAUS4-CATchr1423421537chr1134482798755HLA-B44:07TSEDKKGRLF0.80250.7734717
HAUS4-CATchr1423421537chr1134482798755HLA-B44:26TSEDKKGRLF0.80250.7734717
HAUS4-CATchr1423421537chr1134482798755HLA-B44:13TSEDKKGRLF0.80250.7734717
HAUS4-CATchr1423421537chr1134482798755HLA-B57:10VTSEDKKGRLF0.99990.8683617
HAUS4-CATchr1423421537chr1134482798755HLA-B57:04VTSEDKKGRLF0.99980.5947617
HAUS4-CATchr1423421537chr1134482798755HLA-B44:26SEDKKGRLFAY0.99970.7573819
HAUS4-CATchr1423421537chr1134482798755HLA-B44:07SEDKKGRLFAY0.99970.7573819
HAUS4-CATchr1423421537chr1134482798755HLA-B58:06VTSEDKKGRLF0.99970.5357617
HAUS4-CATchr1423421537chr1134482798755HLA-B44:13SEDKKGRLFAY0.99970.7573819
HAUS4-CATchr1423421537chr1134482798755HLA-B57:02VTSEDKKGRLF0.99960.6481617
HAUS4-CATchr1423421537chr1134482798755HLA-B18:11SEDKKGRLFAY0.99220.624819

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Potential FusionNeoAntigen Information of HAUS4-CAT in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
HAUS4-CAT_23421537_34482798.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
HAUS4-CATchr1423421537chr1134482798755DRB1-1371DTNVTSEDKKGRLFA318
HAUS4-CATchr1423421537chr1134482798755DRB1-1501KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1505KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1506KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1509KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1510KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1513KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1516KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1520KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1522KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1524KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1528KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1532KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1533KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1535KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1537KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1537DKKGRLFAYPDTHRH1025
HAUS4-CATchr1423421537chr1134482798755DRB1-1540KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1541KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1542KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1543KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1545KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB1-1546KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB5-0106KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB5-0106DKKGRLFAYPDTHRH1025
HAUS4-CATchr1423421537chr1134482798755DRB5-0111KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB5-0202KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB5-0202DKKGRLFAYPDTHRH1025
HAUS4-CATchr1423421537chr1134482798755DRB5-0203KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB5-0204KKGRLFAYPDTHRHR1126
HAUS4-CATchr1423421537chr1134482798755DRB5-0205KKGRLFAYPDTHRHR1126

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Fusion breakpoint peptide structures of HAUS4-CAT

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
10327VTSEDKKGRLFAYPHAUS4CATchr1423421537chr1134482798755

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of HAUS4-CAT

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN10327VTSEDKKGRLFAYP-7.15543-7.26883
HLA-B14:023BVN10327VTSEDKKGRLFAYP-4.77435-5.80965
HLA-B52:013W3910327VTSEDKKGRLFAYP-6.80875-6.92215
HLA-B52:013W3910327VTSEDKKGRLFAYP-4.20386-5.23916
HLA-A11:014UQ210327VTSEDKKGRLFAYP-7.5194-8.5547
HLA-A11:014UQ210327VTSEDKKGRLFAYP-6.9601-7.0735
HLA-A24:025HGA10327VTSEDKKGRLFAYP-7.52403-7.63743
HLA-A24:025HGA10327VTSEDKKGRLFAYP-5.82433-6.85963
HLA-B27:056PYJ10327VTSEDKKGRLFAYP-3.28285-4.31815
HLA-B44:053DX810327VTSEDKKGRLFAYP-5.91172-6.94702
HLA-B44:053DX810327VTSEDKKGRLFAYP-4.24346-4.35686
HLA-B35:011A1N10327VTSEDKKGRLFAYP-5.9251-6.0385
HLA-B35:011A1N10327VTSEDKKGRLFAYP-4.80237-5.83767

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Vaccine Design for the FusionNeoAntigens of HAUS4-CAT

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
HAUS4-CATchr1423421537chr11344827981019DKKGRLFAYGACAAAAAGGGCCGCCTTTTTGCCTAT
HAUS4-CATchr1423421537chr1134482798617VTSEDKKGRLFGTAACTTCTGAGGACAAAAAGGGCCGCCTTTTT
HAUS4-CATchr1423421537chr1134482798717TSEDKKGRLFACTTCTGAGGACAAAAAGGGCCGCCTTTTT
HAUS4-CATchr1423421537chr1134482798817SEDKKGRLFTCTGAGGACAAAAAGGGCCGCCTTTTT
HAUS4-CATchr1423421537chr1134482798819SEDKKGRLFAYTCTGAGGACAAAAAGGGCCGCCTTTTTGCCTAT

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
HAUS4-CATchr1423421537chr11344827981025DKKGRLFAYPDTHRHGACAAAAAGGGCCGCCTTTTTGCCTATCCTGACACTCACCGCCAT
HAUS4-CATchr1423421537chr11344827981126KKGRLFAYPDTHRHRAAAAAGGGCCGCCTTTTTGCCTATCCTGACACTCACCGCCATCGC
HAUS4-CATchr1423421537chr1134482798318DTNVTSEDKKGRLFAGACACAAATGTAACTTCTGAGGACAAAAAGGGCCGCCTTTTTGCC

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Information of the samples that have these potential fusion neoantigens of HAUS4-CAT

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
THCAHAUS4-CATchr1423421537ENST00000347758chr1134482798ENST00000241052TCGA-DJ-A1QH-01A

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Potential target of CAR-T therapy development for HAUS4-CAT

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to HAUS4-CAT

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to HAUS4-CAT

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource