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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:ANK3-TES

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ANK3-TES
FusionPDB ID: 4387
FusionGDB2.0 ID: 4387
HgeneTgene
Gene symbol

ANK3

TES

Gene ID

288

26136

Gene nameankyrin 3testin LIM domain protein
SynonymsANKYRIN-G|MRT37TESS|TESS-2
Cytomap

10q21.2

7q31.2

Type of geneprotein-codingprotein-coding
Descriptionankyrin-3ankyrin 3, node of Ranvier (ankyrin G)testintestis derived transcript (3 LIM domains)
Modification date2020031320200313
UniProtAcc

Q12955

Main function of 5'-partner protein: FUNCTION: In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368). {ECO:0000250, ECO:0000269|PubMed:17974005}.; FUNCTION: [Isoform 5]: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. {ECO:0000305|PubMed:17974005}.

PRSS21

Main function of 5'-partner protein: 314
Ensembl transtripts involved in fusion geneENST idsENST00000280772, ENST00000373827, 
ENST00000503366, ENST00000355288, 
ENST00000460468, ENST00000510382, 
ENST00000485009, ENST00000393481, 
ENST00000537767, ENST00000358204, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score28 X 22 X 9=55449 X 6 X 7=378
# samples 2910
** MAII scorelog2(29/5544*10)=-4.25680245212228
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(10/378*10)=-1.91838623444635
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: ANK3 [Title/Abstract] AND TES [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: ANK3 [Title/Abstract] AND TES [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ANK3(61994445)-TES(115874587), # samples:1
Anticipated loss of major functional domain due to fusion event.ANK3-TES seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ANK3-TES seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ANK3-TES seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ANK3-TES seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:61994445/chr7:115874587)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across ANK3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across TES (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000280772ANK3chr1061994445-ENST00000358204TESchr7115874587+362910891922327711
ENST00000373827ANK3chr1061994445-ENST00000358204TESchr7115874587+35901050242288754
ENST00000503366ANK3chr1061994445-ENST00000358204TESchr7115874587+35229821362220694

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000280772ENST00000358204ANK3chr1061994445-TESchr7115874587+0.0020909610.99790907
ENST00000373827ENST00000358204ANK3chr1061994445-TESchr7115874587+0.0016373910.9983626
ENST00000503366ENST00000358204ANK3chr1061994445-TESchr7115874587+0.0010785610.9989215

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ANK3-TES

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
ANK3chr1061994445TESchr71158745871050342LLDRGAKIDAKTRMGLGHEQGFGAPC
ANK3chr1061994445TESchr71158745871089299LLDRGAKIDAKTRMGLGHEQGFGAPC
ANK3chr1061994445TESchr7115874587982282LLDRGAKIDAKTRMGLGHEQGFGAPC

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Potential FusionNeoAntigen Information of ANK3-TES in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ANK3-TES_61994445_115874587.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ANK3-TESchr1061994445chr71158745871089HLA-B15:03AKIDAKTRM0.55130.6683514
ANK3-TESchr1061994445chr71158745871089HLA-B27:04TRMGLGHEQGF10.65971122
ANK3-TESchr1061994445chr71158745871089HLA-B27:07TRMGLGHEQGF0.99990.56171122
ANK3-TESchr1061994445chr71158745871089HLA-C05:09KIDAKTRM10.8538614
ANK3-TESchr1061994445chr71158745871089HLA-C08:15KIDAKTRM0.99990.9327614
ANK3-TESchr1061994445chr71158745871089HLA-C05:01KIDAKTRM10.8538614
ANK3-TESchr1061994445chr71158745871089HLA-C04:03KIDAKTRM10.6601614
ANK3-TESchr1061994445chr71158745871089HLA-C08:02KIDAKTRM0.99990.9327614
ANK3-TESchr1061994445chr71158745871089HLA-B48:02AKIDAKTRM0.41840.8544514
ANK3-TESchr1061994445chr71158745871089HLA-B15:54AKIDAKTRM0.04470.7968514
ANK3-TESchr1061994445chr71158745871089HLA-B15:68AKIDAKTRM0.04370.5572514
ANK3-TESchr1061994445chr71158745871089HLA-B27:10TRMGLGHEQGF10.74811122
ANK3-TESchr1061994445chr71158745871089HLA-B27:06TRMGLGHEQGF10.68891122

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Potential FusionNeoAntigen Information of ANK3-TES in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ANK3-TES_61994445_115874587.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ANK3-TESchr1061994445chr71158745871089DRB1-1102GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1103GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1116GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1136GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1148GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1155GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1155RGAKIDAKTRMGLGH318
ANK3-TESchr1061994445chr71158745871089DRB1-1163GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1165GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1170GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1176GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1185GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1301GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1308GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1320GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1322GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1324GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1335GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1343GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1351GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1352GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1354GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1359GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1364GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1368GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1369GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1370GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1372GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1376GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1378GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1379GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1380GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1383GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1384GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1387GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1391GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1392GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1398GAKIDAKTRMGLGHE419
ANK3-TESchr1061994445chr71158745871089DRB1-1416GAKIDAKTRMGLGHE419

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Fusion breakpoint peptide structures of ANK3-TES

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
4288KIDAKTRMGLGHEQANK3TESchr1061994445chr71158745871089

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ANK3-TES

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN4288KIDAKTRMGLGHEQ-6.15238-6.26578
HLA-B14:023BVN4288KIDAKTRMGLGHEQ-5.35203-6.38733
HLA-B52:013W394288KIDAKTRMGLGHEQ-5.804-6.8393
HLA-B52:013W394288KIDAKTRMGLGHEQ-5.45277-5.56617
HLA-A11:014UQ24288KIDAKTRMGLGHEQ-8.28119-9.31649
HLA-A24:025HGA4288KIDAKTRMGLGHEQ-9.93626-10.0497
HLA-A24:025HGA4288KIDAKTRMGLGHEQ-5.67154-6.70684
HLA-B27:056PYJ4288KIDAKTRMGLGHEQ-4.30966-5.34496
HLA-B44:053DX84288KIDAKTRMGLGHEQ-7.17842-7.29182
HLA-B44:053DX84288KIDAKTRMGLGHEQ-4.14208-5.17738
HLA-A02:016TDR4288KIDAKTRMGLGHEQ-5.17192-5.28532

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Vaccine Design for the FusionNeoAntigens of ANK3-TES

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
ANK3-TESchr1061994445chr71158745871122TRMGLGHEQGFACCAGGATGGGCTTAGGTCACGAGCAAGGATTT
ANK3-TESchr1061994445chr7115874587514AKIDAKTRMGCTAAAATCGATGCCAAAACCAGGATG
ANK3-TESchr1061994445chr7115874587614KIDAKTRMAAAATCGATGCCAAAACCAGGATG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
ANK3-TESchr1061994445chr7115874587318RGAKIDAKTRMGLGHCGAGGAGCTAAAATCGATGCCAAAACCAGGATGGGCTTAGGTCAC
ANK3-TESchr1061994445chr7115874587419GAKIDAKTRMGLGHEGGAGCTAAAATCGATGCCAAAACCAGGATGGGCTTAGGTCACGAG

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Information of the samples that have these potential fusion neoantigens of ANK3-TES

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
STADANK3-TESchr1061994445ENST00000280772chr7115874587ENST00000358204TCGA-HU-A4GQ

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Potential target of CAR-T therapy development for ANK3-TES

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to ANK3-TES

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ANK3-TES

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource