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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:ANO1-UNC5D

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ANO1-UNC5D
FusionPDB ID: 4867
FusionGDB2.0 ID: 4867
HgeneTgene
Gene symbol

ANO1

UNC5D

Gene ID

55107

137970

Gene nameanoctamin 1unc-5 netrin receptor D
SynonymsDOG1|ORAOV2|TAOS2|TMEM16APRO34692|Unc5h4
Cytomap

11q13.3

8p12

Type of geneprotein-codingprotein-coding
Descriptionanoctamin-1Ca2+-activated Cl- channelanoctamin 1, calcium activated chloride channelcalcium activated chloride channeldiscovered on gastrointestinal stromal tumors protein 1oral cancer overexpressed 2transmembrane protein 16A (eight membrane-spanninnetrin receptor UNC5Dnetrin receptor Unc5h4protein unc-5 homolog 4protein unc-5 homolog Dunc-5 homolog 4unc-5 homolog D
Modification date2020031320200313
UniProtAcc

Q9NW15

Main function of 5'-partner protein: FUNCTION: Does not exhibit calcium-activated chloride channel (CaCC) activity. Can inhibit the activity of ANO1. {ECO:0000269|PubMed:20056604, ECO:0000269|PubMed:22946059}.		.
Ensembl transtripts involved in fusion geneENST idsENST00000525494, ENST00000355303, 
ENST00000398543, ENST00000530676, 
ENST00000531349, ENST00000538023, 
ENST00000316296, 		ENST00000287272, 
ENST00000404895, ENST00000416672, 
ENST00000420357, ENST00000453357, 
ENST00000449677, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score20 X 18 X 9=324017 X 13 X 6=1326
# samples 2020
** MAII scorelog2(20/3240*10)=-4.01792190799726
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(20/1326*10)=-2.72900887033786
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: ANO1 [Title/Abstract] AND UNC5D [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: ANO1 [Title/Abstract] AND UNC5D [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ANO1(70017145)-UNC5D(35406810), # samples:3
Anticipated loss of major functional domain due to fusion event.ANO1-UNC5D seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ANO1-UNC5D seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneANO1

GO:0006812

cation transport

22946059

TgeneUNC5D

GO:0098742

cell-cell adhesion via plasma-membrane adhesion molecules

26235030



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:70017145/chr8:35406810)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across ANO1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across UNC5D (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000355303ANO1chr1170017145+ENST00000287272UNC5Dchr835406810+11194265522152061661
ENST00000355303ANO1chr1170017145+ENST00000453357UNC5Dchr835406810+9513265522154131730
ENST00000355303ANO1chr1170017145+ENST00000404895UNC5Dchr835406810+5476265522154131730
ENST00000355303ANO1chr1170017145+ENST00000420357UNC5Dchr835406810+5498265522152121663
ENST00000355303ANO1chr1170017145+ENST00000416672UNC5Dchr835406810+6074265522154281735
ENST00000398543ANO1chr1170017145+ENST00000287272UNC5Dchr835406810+1084823094948601603
ENST00000398543ANO1chr1170017145+ENST00000453357UNC5Dchr835406810+916723094950671672
ENST00000398543ANO1chr1170017145+ENST00000404895UNC5Dchr835406810+513023094950671672
ENST00000398543ANO1chr1170017145+ENST00000420357UNC5Dchr835406810+515223094948661605
ENST00000398543ANO1chr1170017145+ENST00000416672UNC5Dchr835406810+572823094950821677
ENST00000538023ANO1chr1170017145+ENST00000287272UNC5Dchr835406810+1093823994949501633
ENST00000538023ANO1chr1170017145+ENST00000453357UNC5Dchr835406810+925723994951571702
ENST00000538023ANO1chr1170017145+ENST00000404895UNC5Dchr835406810+522023994951571702
ENST00000538023ANO1chr1170017145+ENST00000420357UNC5Dchr835406810+524223994949561635
ENST00000538023ANO1chr1170017145+ENST00000416672UNC5Dchr835406810+581823994951721707
ENST00000530676ANO1chr1170017145+ENST00000287272UNC5Dchr835406810+10940240124949521567
ENST00000530676ANO1chr1170017145+ENST00000453357UNC5Dchr835406810+9259240124951591636
ENST00000530676ANO1chr1170017145+ENST00000404895UNC5Dchr835406810+5222240124951591636
ENST00000530676ANO1chr1170017145+ENST00000420357UNC5Dchr835406810+5244240124949581569
ENST00000530676ANO1chr1170017145+ENST00000416672UNC5Dchr835406810+5820240124951741641
ENST00000531349ANO1chr1170017145+ENST00000287272UNC5Dchr835406810+10232169318042441354
ENST00000531349ANO1chr1170017145+ENST00000453357UNC5Dchr835406810+8551169318044511423
ENST00000531349ANO1chr1170017145+ENST00000404895UNC5Dchr835406810+4514169318044511423
ENST00000531349ANO1chr1170017145+ENST00000420357UNC5Dchr835406810+4536169318042501356
ENST00000531349ANO1chr1170017145+ENST00000416672UNC5Dchr835406810+5112169318044661428
ENST00000355303ANO1chr1170017145+ENST00000287272UNC5Dchr835406809+11194265522152061661
ENST00000355303ANO1chr1170017145+ENST00000453357UNC5Dchr835406809+9513265522154131730
ENST00000355303ANO1chr1170017145+ENST00000404895UNC5Dchr835406809+5476265522154131730
ENST00000355303ANO1chr1170017145+ENST00000420357UNC5Dchr835406809+5498265522152121663
ENST00000355303ANO1chr1170017145+ENST00000416672UNC5Dchr835406809+6074265522154281735
ENST00000398543ANO1chr1170017145+ENST00000287272UNC5Dchr835406809+1084823094948601603
ENST00000398543ANO1chr1170017145+ENST00000453357UNC5Dchr835406809+916723094950671672
ENST00000398543ANO1chr1170017145+ENST00000404895UNC5Dchr835406809+513023094950671672
ENST00000398543ANO1chr1170017145+ENST00000420357UNC5Dchr835406809+515223094948661605
ENST00000398543ANO1chr1170017145+ENST00000416672UNC5Dchr835406809+572823094950821677
ENST00000538023ANO1chr1170017145+ENST00000287272UNC5Dchr835406809+1093823994949501633
ENST00000538023ANO1chr1170017145+ENST00000453357UNC5Dchr835406809+925723994951571702
ENST00000538023ANO1chr1170017145+ENST00000404895UNC5Dchr835406809+522023994951571702
ENST00000538023ANO1chr1170017145+ENST00000420357UNC5Dchr835406809+524223994949561635
ENST00000538023ANO1chr1170017145+ENST00000416672UNC5Dchr835406809+581823994951721707
ENST00000530676ANO1chr1170017145+ENST00000287272UNC5Dchr835406809+10940240124949521567
ENST00000530676ANO1chr1170017145+ENST00000453357UNC5Dchr835406809+9259240124951591636
ENST00000530676ANO1chr1170017145+ENST00000404895UNC5Dchr835406809+5222240124951591636
ENST00000530676ANO1chr1170017145+ENST00000420357UNC5Dchr835406809+5244240124949581569
ENST00000530676ANO1chr1170017145+ENST00000416672UNC5Dchr835406809+5820240124951741641
ENST00000531349ANO1chr1170017145+ENST00000287272UNC5Dchr835406809+10232169318042441354
ENST00000531349ANO1chr1170017145+ENST00000453357UNC5Dchr835406809+8551169318044511423
ENST00000531349ANO1chr1170017145+ENST00000404895UNC5Dchr835406809+4514169318044511423
ENST00000531349ANO1chr1170017145+ENST00000420357UNC5Dchr835406809+4536169318042501356
ENST00000531349ANO1chr1170017145+ENST00000416672UNC5Dchr835406809+5112169318044661428

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000355303ENST00000287272ANO1chr1170017145+UNC5Dchr835406810+0.0001292290.9998708
ENST00000355303ENST00000453357ANO1chr1170017145+UNC5Dchr835406810+0.0003469410.9996531
ENST00000355303ENST00000404895ANO1chr1170017145+UNC5Dchr835406810+0.0017215680.9982785
ENST00000355303ENST00000420357ANO1chr1170017145+UNC5Dchr835406810+0.0020773130.9979227
ENST00000355303ENST00000416672ANO1chr1170017145+UNC5Dchr835406810+0.0011032370.9988967
ENST00000398543ENST00000287272ANO1chr1170017145+UNC5Dchr835406810+8.94E-050.9999106
ENST00000398543ENST00000453357ANO1chr1170017145+UNC5Dchr835406810+0.0002276780.99977237
ENST00000398543ENST00000404895ANO1chr1170017145+UNC5Dchr835406810+0.0012293920.99877053
ENST00000398543ENST00000420357ANO1chr1170017145+UNC5Dchr835406810+0.0015562830.9984438
ENST00000398543ENST00000416672ANO1chr1170017145+UNC5Dchr835406810+0.0007759640.99922407
ENST00000538023ENST00000287272ANO1chr1170017145+UNC5Dchr835406810+9.23E-050.99990773
ENST00000538023ENST00000453357ANO1chr1170017145+UNC5Dchr835406810+0.0002350190.999765
ENST00000538023ENST00000404895ANO1chr1170017145+UNC5Dchr835406810+0.001274890.9987251
ENST00000538023ENST00000420357ANO1chr1170017145+UNC5Dchr835406810+0.0016023480.99839765
ENST00000538023ENST00000416672ANO1chr1170017145+UNC5Dchr835406810+0.0008025570.9991974
ENST00000530676ENST00000287272ANO1chr1170017145+UNC5Dchr835406810+0.000146940.999853
ENST00000530676ENST00000453357ANO1chr1170017145+UNC5Dchr835406810+0.0003820880.99961793
ENST00000530676ENST00000404895ANO1chr1170017145+UNC5Dchr835406810+0.001942780.9980572
ENST00000530676ENST00000420357ANO1chr1170017145+UNC5Dchr835406810+0.0024351780.9975648
ENST00000530676ENST00000416672ANO1chr1170017145+UNC5Dchr835406810+0.0012313140.9987686
ENST00000531349ENST00000287272ANO1chr1170017145+UNC5Dchr835406810+8.25E-050.9999175
ENST00000531349ENST00000453357ANO1chr1170017145+UNC5Dchr835406810+0.0002052490.9997948
ENST00000531349ENST00000404895ANO1chr1170017145+UNC5Dchr835406810+0.001060350.9989397
ENST00000531349ENST00000420357ANO1chr1170017145+UNC5Dchr835406810+0.001390980.998609
ENST00000531349ENST00000416672ANO1chr1170017145+UNC5Dchr835406810+0.000653470.99934655
ENST00000355303ENST00000287272ANO1chr1170017145+UNC5Dchr835406809+0.0001292290.9998708
ENST00000355303ENST00000453357ANO1chr1170017145+UNC5Dchr835406809+0.0003469410.9996531
ENST00000355303ENST00000404895ANO1chr1170017145+UNC5Dchr835406809+0.0017215680.9982785
ENST00000355303ENST00000420357ANO1chr1170017145+UNC5Dchr835406809+0.0020773130.9979227
ENST00000355303ENST00000416672ANO1chr1170017145+UNC5Dchr835406809+0.0011032370.9988967
ENST00000398543ENST00000287272ANO1chr1170017145+UNC5Dchr835406809+8.94E-050.9999106
ENST00000398543ENST00000453357ANO1chr1170017145+UNC5Dchr835406809+0.0002276780.99977237
ENST00000398543ENST00000404895ANO1chr1170017145+UNC5Dchr835406809+0.0012293920.99877053
ENST00000398543ENST00000420357ANO1chr1170017145+UNC5Dchr835406809+0.0015562830.9984438
ENST00000398543ENST00000416672ANO1chr1170017145+UNC5Dchr835406809+0.0007759640.99922407
ENST00000538023ENST00000287272ANO1chr1170017145+UNC5Dchr835406809+9.23E-050.99990773
ENST00000538023ENST00000453357ANO1chr1170017145+UNC5Dchr835406809+0.0002350190.999765
ENST00000538023ENST00000404895ANO1chr1170017145+UNC5Dchr835406809+0.001274890.9987251
ENST00000538023ENST00000420357ANO1chr1170017145+UNC5Dchr835406809+0.0016023480.99839765
ENST00000538023ENST00000416672ANO1chr1170017145+UNC5Dchr835406809+0.0008025570.9991974
ENST00000530676ENST00000287272ANO1chr1170017145+UNC5Dchr835406809+0.000146940.999853
ENST00000530676ENST00000453357ANO1chr1170017145+UNC5Dchr835406809+0.0003820880.99961793
ENST00000530676ENST00000404895ANO1chr1170017145+UNC5Dchr835406809+0.001942780.9980572
ENST00000530676ENST00000420357ANO1chr1170017145+UNC5Dchr835406809+0.0024351780.9975648
ENST00000530676ENST00000416672ANO1chr1170017145+UNC5Dchr835406809+0.0012313140.9987686
ENST00000531349ENST00000287272ANO1chr1170017145+UNC5Dchr835406809+8.25E-050.9999175
ENST00000531349ENST00000453357ANO1chr1170017145+UNC5Dchr835406809+0.0002052490.9997948
ENST00000531349ENST00000404895ANO1chr1170017145+UNC5Dchr835406809+0.001060350.9989397
ENST00000531349ENST00000420357ANO1chr1170017145+UNC5Dchr835406809+0.001390980.998609
ENST00000531349ENST00000416672ANO1chr1170017145+UNC5Dchr835406809+0.000653470.99934655

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ANO1-UNC5D

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
ANO1chr1170017145UNC5Dchr8354068091693504ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068092309753ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068092399783ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068092401717ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068092655811ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068101693504ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068102309753ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068102399783ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068102401717ELRRPVAVRAKDIGTDNGEALPESIP
ANO1chr1170017145UNC5Dchr8354068102655811ELRRPVAVRAKDIGTDNGEALPESIP

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Potential FusionNeoAntigen Information of ANO1-UNC5D in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ANO1-UNC5D_70017145_35406809.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ANO1-UNC5Dchr1170017145chr8354068092655HLA-C08:03IGTDNGEAL0.4590.97821221
ANO1-UNC5Dchr1170017145chr8354068092655HLA-C08:01IGTDNGEAL0.4590.97821221
ANO1-UNC5Dchr1170017145chr8354068092655HLA-B35:13IGTDNGEAL0.4340.91081221

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Potential FusionNeoAntigen Information of ANO1-UNC5D in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ANO1-UNC5D_70017145_35406809.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0801PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0806PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0806RPVAVRAKDIGTDNG318
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0806RRPVAVRAKDIGTDN217
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0810PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0810RPVAVRAKDIGTDNG318
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0812PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0812RPVAVRAKDIGTDNG318
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0816PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0822PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0822RPVAVRAKDIGTDNG318
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0822RRPVAVRAKDIGTDN217
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0826PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-0839PVAVRAKDIGTDNGE419
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-1155RRPVAVRAKDIGTDN217
ANO1-UNC5Dchr1170017145chr8354068092655DRB1-1159RRPVAVRAKDIGTDN217
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0101AKDIGTDNGEALPES924
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0101RAKDIGTDNGEALPE823
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0104AKDIGTDNGEALPES924
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0104RAKDIGTDNGEALPE823
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0105AKDIGTDNGEALPES924
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0108AKDIGTDNGEALPES924
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0108RAKDIGTDNGEALPE823
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0111AKDIGTDNGEALPES924
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0111RAKDIGTDNGEALPE823
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0112AKDIGTDNGEALPES924
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0112RAKDIGTDNGEALPE823
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0113AKDIGTDNGEALPES924
ANO1-UNC5Dchr1170017145chr8354068092655DRB3-0113RAKDIGTDNGEALPE823

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Fusion breakpoint peptide structures of ANO1-UNC5D

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
705AVRAKDIGTDNGEAANO1UNC5Dchr1170017145chr8354068092655

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ANO1-UNC5D

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN705AVRAKDIGTDNGEA-7.15543-7.26883
HLA-B14:023BVN705AVRAKDIGTDNGEA-4.77435-5.80965
HLA-B52:013W39705AVRAKDIGTDNGEA-6.80875-6.92215
HLA-B52:013W39705AVRAKDIGTDNGEA-4.20386-5.23916
HLA-A11:014UQ2705AVRAKDIGTDNGEA-7.5194-8.5547
HLA-A11:014UQ2705AVRAKDIGTDNGEA-6.9601-7.0735
HLA-A24:025HGA705AVRAKDIGTDNGEA-7.52403-7.63743
HLA-A24:025HGA705AVRAKDIGTDNGEA-5.82433-6.85963
HLA-B27:056PYJ705AVRAKDIGTDNGEA-3.28285-4.31815
HLA-B44:053DX8705AVRAKDIGTDNGEA-5.91172-6.94702
HLA-B44:053DX8705AVRAKDIGTDNGEA-4.24346-4.35686

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Vaccine Design for the FusionNeoAntigens of ANO1-UNC5D

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
ANO1-UNC5Dchr1170017145chr8354068091221IGTDNGEALTCGGAACTGACAATGGCGAAGCCCTTC

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
ANO1-UNC5Dchr1170017145chr835406809217RRPVAVRAKDIGTDNGAAGGCCGGTAGCTGTCAGAGCCAAAGACATCGGAACTGACAATG
ANO1-UNC5Dchr1170017145chr835406809318RPVAVRAKDIGTDNGGGCCGGTAGCTGTCAGAGCCAAAGACATCGGAACTGACAATGGCG
ANO1-UNC5Dchr1170017145chr835406809419PVAVRAKDIGTDNGECGGTAGCTGTCAGAGCCAAAGACATCGGAACTGACAATGGCGAAG
ANO1-UNC5Dchr1170017145chr835406809823RAKDIGTDNGEALPEGAGCCAAAGACATCGGAACTGACAATGGCGAAGCCCTTCCCGAAT
ANO1-UNC5Dchr1170017145chr835406809924AKDIGTDNGEALPESCCAAAGACATCGGAACTGACAATGGCGAAGCCCTTCCCGAATCCA

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Information of the samples that have these potential fusion neoantigens of ANO1-UNC5D

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BRCAANO1-UNC5Dchr1170017145ENST00000355303chr835406809ENST00000287272TCGA-E9-A228

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Potential target of CAR-T therapy development for ANO1-UNC5D

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226334_354783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226407_427783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226520_540783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226569_589783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226608_628783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226658_678783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226726_746783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000355303+2226747_767783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000398543+2024334_354637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000398543+2024407_427637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000398543+2024520_540637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000398543+2024569_589637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000398543+2024608_628637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000530676+2025334_354637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000530676+2025407_427637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000530676+2025520_540637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000530676+2025569_589637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406809ENST00000530676+2025608_628637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226334_354783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226407_427783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226520_540783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226569_589783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226608_628783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226658_678783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226726_746783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000355303+2226747_767783987.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000398543+2024334_354637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000398543+2024407_427637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000398543+2024520_540637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000398543+2024569_589637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000398543+2024608_628637841.0TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000530676+2025334_354637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000530676+2025407_427637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000530676+2025520_540637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000530676+2025569_589637819.3333333333334TransmembraneHelical
HgeneANO1chr11:70017145chr8:35406810ENST00000530676+2025608_628637819.3333333333334TransmembraneHelical
TgeneUNC5Dchr11:70017145chr8:35406809ENST00000404895017380_4000954.0TransmembraneHelical
TgeneUNC5Dchr11:70017145chr8:35406809ENST00000453357017380_4000949.0TransmembraneHelical
TgeneUNC5Dchr11:70017145chr8:35406810ENST00000404895017380_4000954.0TransmembraneHelical
TgeneUNC5Dchr11:70017145chr8:35406810ENST00000453357017380_4000949.0TransmembraneHelical

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to ANO1-UNC5D

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ANO1-UNC5D

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource