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Fusion Protein:NF1-RBX1

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NF1-RBX1
	FusionPDB ID: 58700
	FusionGDB2.0 ID: 58700
		Hgene	Tgene
	Gene symbol	NF1	RBX1
	Gene ID	4763	9978
	Gene name	neurofibromin 1	ring-box 1
	Synonyms	NFNS\|VRNF\|WSS	BA554C12.1\|RNF75\|ROC1
	Cytomap	17q11.2	22q13.2
	Type of gene	protein-coding	protein-coding
	Description	neurofibrominneurofibromatosis 1neurofibromatosis-related protein NF-1truncated neurofibromin 1	E3 ubiquitin-protein ligase RBX1E3 ubiquitin-protein transferase RBX1RING finger protein 75RING-box protein 1ZYP proteinregulator of cullins 1ring-box 1, E3 ubiquitin protein ligase
	Modification date	20200322	20200327
	UniProtAcc	P21359 Main function of 5'-partner protein: FUNCTION: Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity. {ECO:0000269\|PubMed:2121371, ECO:0000269\|PubMed:8417346}.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000356175, ENST00000358273, ENST00000431387, ENST00000417592, ENST00000444181, ENST00000581113,	ENST00000216225,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	47 X 26 X 21=25662	11 X 8 X 10=880
	# samples	69	14
	** MAII score	log2(69/25662*10)=-5.21689344093196 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(14/880*10)=-2.65207669657969 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: NF1 [Title/Abstract] AND RBX1 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: NF1 [Title/Abstract] AND RBX1 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NF1(29422387)-RBX1(41349559), # samples:2
Anticipated loss of major functional domain due to fusion event.	NF1-RBX1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NF1-RBX1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NF1-RBX1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NF1-RBX1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NF1-RBX1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. NF1-RBX1 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF. NF1-RBX1 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	NF1	GO:0043547	positive regulation of GTPase activity	2121371
Tgene	RBX1	GO:0006513	protein monoubiquitination	22358839
Tgene	RBX1	GO:0016567	protein ubiquitination	15103331\|17543862\|20389280
Tgene	RBX1	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	15103331
Tgene	RBX1	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	20389280
Tgene	RBX1	GO:0045116	protein neddylation	19250909

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:29422387/chr22:41349559)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across NF1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across RBX1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000356175	NF1	chr17	29422387	+	ENST00000216225	RBX1	chr22	41349559	+	1513	443	254	691	145
ENST00000358273	NF1	chr17	29422387	+	ENST00000216225	RBX1	chr22	41349559	+	1513	443	254	691	145
ENST00000431387	NF1	chr17	29422387	+	ENST00000216225	RBX1	chr22	41349559	+	1463	393	204	641	145
ENST00000356175	NF1	chr17	29422387	+	ENST00000216225	RBX1	chr22	41349558	+	1513	443	254	691	145
ENST00000358273	NF1	chr17	29422387	+	ENST00000216225	RBX1	chr22	41349558	+	1513	443	254	691	145
ENST00000431387	NF1	chr17	29422387	+	ENST00000216225	RBX1	chr22	41349558	+	1463	393	204	641	145

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000356175	ENST00000216225	NF1	chr17	29422387	+	RBX1	chr22	41349559	+	0.30897817	0.69102186
ENST00000358273	ENST00000216225	NF1	chr17	29422387	+	RBX1	chr22	41349559	+	0.30897817	0.69102186
ENST00000431387	ENST00000216225	NF1	chr17	29422387	+	RBX1	chr22	41349559	+	0.3881853	0.61181474
ENST00000356175	ENST00000216225	NF1	chr17	29422387	+	RBX1	chr22	41349558	+	0.30897817	0.69102186
ENST00000358273	ENST00000216225	NF1	chr17	29422387	+	RBX1	chr22	41349558	+	0.30897817	0.69102186
ENST00000431387	ENST00000216225	NF1	chr17	29422387	+	RBX1	chr22	41349558	+	0.3881853	0.61181474

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for NF1-RBX1

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
NF1	chr17	29422387	RBX1	chr22	41349558	393	62	VEWVQAVVSRFDEQWNAVALWAWDIV
NF1	chr17	29422387	RBX1	chr22	41349558	443	62	VEWVQAVVSRFDEQWNAVALWAWDIV
NF1	chr17	29422387	RBX1	chr22	41349559	393	62	VEWVQAVVSRFDEQWNAVALWAWDIV
NF1	chr17	29422387	RBX1	chr22	41349559	443	62	VEWVQAVVSRFDEQWNAVALWAWDIV

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Potential FusionNeoAntigen Information of NF1-RBX1 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

NF1-RBX1_29422387_41349558.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:01	VSRFDEQW	0.9998	0.9945	7	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:02	VSRFDEQW	0.9989	0.9848	7	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:01	VSRFDEQW	0.9983	0.9887	7	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:01	EQWNAVAL	0.9952	0.9777	12	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:10	EQWNAVAL	0.9815	0.7657	12	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:37	EQWNAVAL	0.9788	0.801	12	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:07	SRFDEQWNA	0.9995	0.7407	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:04	SRFDEQWNA	0.9993	0.7946	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:05	SRFDEQWNA	0.9992	0.9409	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:01	VVSRFDEQW	0.9979	0.9941	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:06	SRFDEQWNA	0.997	0.9343	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:01	VVSRFDEQW	0.9951	0.9878	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:24	SRFDEQWNA	0.9949	0.7173	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:02	VVSRFDEQW	0.9927	0.9868	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:01	SRFDEQWNA	0.9887	0.9614	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:17	VVSRFDEQW	0.9858	0.9597	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:01	DEQWNAVAL	0.979	0.9384	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:03	EQWNAVALW	0.9713	0.9911	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:03	VVSRFDEQW	0.9458	0.9961	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:37	DEQWNAVAL	0.8851	0.8322	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:16	VVSRFDEQW	0.8751	0.941	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:02	EQWNAVALW	0.8686	0.7365	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-A32:13	VVSRFDEQW	0.8275	0.9592	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B53:01	EQWNAVALW	0.8264	0.8701	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B53:01	VVSRFDEQW	0.729	0.6159	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-A32:13	EQWNAVALW	0.7069	0.992	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B13:01	EQWNAVALW	0.2028	0.9965	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:02	SRFDEQWNAV	0.9999	0.8623	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:05	SRFDEQWNAV	0.9999	0.9495	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:04	SRFDEQWNAV	0.9999	0.8048	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:07	SRFDEQWNAV	0.9998	0.8755	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:01	AVVSRFDEQW	0.9995	0.9954	5	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:06	SRFDEQWNAV	0.9987	0.9617	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:24	SRFDEQWNAV	0.9982	0.8229	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:03	DEQWNAVALW	0.998	0.987	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:01	AVVSRFDEQW	0.997	0.991	5	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:02	DEQWNAVALW	0.9946	0.5385	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:05	DEQWNAVALW	0.9724	0.5952	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:03	AVVSRFDEQW	0.9718	0.9952	5	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-A32:13	AVVSRFDEQW	0.7982	0.9677	5	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:01	QAVVSRFDEQW	0.9999	0.9929	4	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:06	SRFDEQWNAVA	0.9997	0.9829	8	19
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:02	QAVVSRFDEQW	0.9996	0.9879	4	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:01	QAVVSRFDEQW	0.9991	0.9847	4	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:03	QAVVSRFDEQW	0.9979	0.9937	4	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B40:01	RFDEQWNAVAL	0.9061	0.6695	9	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:05	EQWNAVAL	0.9933	0.9702	12	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:14	SRFDEQWNA	0.9993	0.8539	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:10	RFDEQWNAV	0.9993	0.9874	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:07	RFDEQWNAV	0.9992	0.9874	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B73:01	SRFDEQWNA	0.9978	0.9487	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:09	SRFDEQWNA	0.9887	0.8628	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:12	SRFDEQWNA	0.9883	0.9651	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C07:95	SRFDEQWNA	0.9769	0.8874	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:03	SRFDEQWNA	0.9655	0.9448	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:10	DEQWNAVAL	0.9589	0.6043	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:08	EQWNAVALW	0.9025	0.8769	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:06	EQWNAVALW	0.8451	0.5507	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:04	EQWNAVALW	0.8402	0.6676	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:14	RFDEQWNAV	0.7423	0.9862	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B14:03	DEQWNAVAL	0.6329	0.9439	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:09	DEQWNAVAL	0.5872	0.9177	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:05	DEQWNAVAL	0.569	0.9592	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:14	SRFDEQWNAV	0.9999	0.8755	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B73:01	SRFDEQWNAV	0.9987	0.978	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:12	SRFDEQWNAV	0.9968	0.9818	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:03	SRFDEQWNAV	0.996	0.9527	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:04	DEQWNAVALW	0.9943	0.544	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:08	DEQWNAVALW	0.992	0.6561	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:14	VSRFDEQWNA	0.9408	0.8139	7	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B73:01	VSRFDEQWNA	0.8111	0.9565	7	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:07	RFDEQWNAVAL	1	0.9651	9	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:14	SRFDEQWNAVA	1	0.8939	8	19
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:10	RFDEQWNAVAL	1	0.9688	9	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B73:01	SRFDEQWNAVA	0.9998	0.9835	8	19
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:14	RFDEQWNAVAL	0.9959	0.9777	9	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B73:01	VVSRFDEQWNA	0.957	0.9628	6	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:10	VSRFDEQW	0.9998	0.9945	7	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:04	VSRFDEQW	0.9992	0.8569	7	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:06	VSRFDEQW	0.9984	0.9634	7	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:09	EQWNAVAL	0.9453	0.794	12	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:04	EQWNAVAL	0.8457	0.9339	12	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:03	RFDEQWNAV	0.9995	0.9897	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:09	SRFDEQWNA	0.9995	0.9321	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:10	SRFDEQWNA	0.9993	0.8879	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:01	RFDEQWNAV	0.9992	0.9874	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:06	SRFDEQWNA	0.9992	0.8033	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:08	SRFDEQWNA	0.9992	0.8501	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C18:01	RFDEQWNAV	0.9987	0.9868	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:10	VVSRFDEQW	0.9979	0.9941	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:04	VVSRFDEQW	0.9944	0.8972	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:31	SRFDEQWNA	0.991	0.9617	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B58:06	VVSRFDEQW	0.9893	0.9676	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:04	DEQWNAVAL	0.9871	0.9453	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:07	DEQWNAVAL	0.9839	0.9081	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:05	DEQWNAVAL	0.979	0.9384	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C07:01	SRFDEQWNA	0.9755	0.8859	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B40:04	DEQWNAVAL	0.9755	0.7766	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:08	DEQWNAVAL	0.9747	0.9447	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:06	DEQWNAVAL	0.9739	0.9429	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C06:08	SRFDEQWNA	0.9736	0.9938	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:13	EQWNAVALW	0.9713	0.9911	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:26	EQWNAVALW	0.9713	0.9911	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:07	EQWNAVALW	0.9713	0.9911	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:03	DEQWNAVAL	0.9712	0.9333	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:02	VVSRFDEQW	0.9553	0.9804	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-A32:01	VVSRFDEQW	0.898	0.9488	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:22	EQWNAVALW	0.8686	0.7365	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:13	VVSRFDEQW	0.8643	0.82	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:04	RFDEQWNAV	0.8447	0.9933	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:13	EQWNAVALW	0.8292	0.9656	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:24	EQWNAVALW	0.8054	0.9825	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B18:11	DEQWNAVAL	0.7475	0.8988	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B53:02	EQWNAVALW	0.7362	0.8995	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:21	EQWNAVALW	0.7171	0.5563	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:11	DEQWNAVAL	0.6271	0.8586	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B53:02	VVSRFDEQW	0.6225	0.5635	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:31	DEQWNAVAL	0.587	0.9703	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C07:22	SRFDEQWNA	0.5524	0.7994	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B41:03	DEQWNAVAL	0.532	0.7112	11	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C07:04	RFDEQWNAV	0.5217	0.9721	9	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-A25:01	EQWNAVALW	0.4493	0.9328	12	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:24	VVSRFDEQW	0.4055	0.9721	6	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C06:02	SRFDEQWNA	0.0302	0.9946	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C06:17	SRFDEQWNA	0.0302	0.9946	8	17
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:06	SRFDEQWNAV	0.9999	0.8084	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:09	SRFDEQWNAV	0.9999	0.9446	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:08	SRFDEQWNAV	0.9999	0.8901	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B27:10	SRFDEQWNAV	0.9999	0.8842	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:10	AVVSRFDEQW	0.9995	0.9954	5	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:04	AVVSRFDEQW	0.9986	0.9078	5	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B39:31	SRFDEQWNAV	0.998	0.9796	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:26	DEQWNAVALW	0.998	0.987	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:07	DEQWNAVALW	0.998	0.987	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:13	DEQWNAVALW	0.998	0.987	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C06:08	SRFDEQWNAV	0.9948	0.9961	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:22	DEQWNAVALW	0.9946	0.5385	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:02	AVVSRFDEQW	0.9762	0.9844	5	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B44:21	DEQWNAVALW	0.939	0.5073	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C06:17	SRFDEQWNAV	0.8724	0.9963	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C06:02	SRFDEQWNAV	0.8724	0.9963	8	18
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B15:13	DEQWNAVALW	0.6952	0.9336	11	21
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C04:01	RFDEQWNAVAL	1	0.9651	9	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:10	QAVVSRFDEQW	0.9999	0.9929	4	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-C18:01	RFDEQWNAVAL	0.9999	0.9593	9	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:04	QAVVSRFDEQW	0.9996	0.8146	4	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B57:02	QAVVSRFDEQW	0.9963	0.9626	4	15
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B40:36	RFDEQWNAVAL	0.942	0.6845	9	20
NF1-RBX1	chr17	29422387	chr22	41349558	443	HLA-B40:49	RFDEQWNAVAL	0.8554	0.6795	9	20

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Potential FusionNeoAntigen Information of NF1-RBX1 in HLA II

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene

Hchr

Hbp

Tgene

Tchr

Tbp

HLA II

FusionNeoAntigen peptide

Neoantigen start (at BP 13)

Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of NF1-RBX1

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
10376	VVSRFDEQWNAVAL	NF1	RBX1	chr17	29422387	chr22	41349558	443

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of NF1-RBX1

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	10376	VVSRFDEQWNAVAL	-7.9962	-8.1096
HLA-B14:02	3BVN	10376	VVSRFDEQWNAVAL	-5.70842	-6.74372
HLA-B52:01	3W39	10376	VVSRFDEQWNAVAL	-6.83737	-6.95077
HLA-B52:01	3W39	10376	VVSRFDEQWNAVAL	-4.4836	-5.5189
HLA-A11:01	4UQ2	10376	VVSRFDEQWNAVAL	-10.0067	-10.1201
HLA-A11:01	4UQ2	10376	VVSRFDEQWNAVAL	-9.03915	-10.0745
HLA-A24:02	5HGA	10376	VVSRFDEQWNAVAL	-6.56204	-6.67544
HLA-A24:02	5HGA	10376	VVSRFDEQWNAVAL	-5.42271	-6.45801
HLA-B44:05	3DX8	10376	VVSRFDEQWNAVAL	-7.85648	-8.89178
HLA-B44:05	3DX8	10376	VVSRFDEQWNAVAL	-5.3978	-5.5112
HLA-B35:01	1A1N	10376	VVSRFDEQWNAVAL	-6.27422	-6.38762
HLA-B35:01	1A1N	10376	VVSRFDEQWNAVAL	-5.27424	-6.30954
HLA-A02:01	6TDR	10376	VVSRFDEQWNAVAL	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of NF1-RBX1

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
NF1-RBX1	chr17	29422387	chr22	41349558	11	20	DEQWNAVAL	GAGCAGTGGAATGCAGTAGCCCTCTGG
NF1-RBX1	chr17	29422387	chr22	41349558	11	21	DEQWNAVALW	GAGCAGTGGAATGCAGTAGCCCTCTGGGCC
NF1-RBX1	chr17	29422387	chr22	41349558	12	20	EQWNAVAL	CAGTGGAATGCAGTAGCCCTCTGG
NF1-RBX1	chr17	29422387	chr22	41349558	12	21	EQWNAVALW	CAGTGGAATGCAGTAGCCCTCTGGGCC
NF1-RBX1	chr17	29422387	chr22	41349558	4	15	QAVVSRFDEQW	GCCGTGGTCAGCCGCTTCGACGAGCAGTGGAAT
NF1-RBX1	chr17	29422387	chr22	41349558	5	15	AVVSRFDEQW	GTGGTCAGCCGCTTCGACGAGCAGTGGAAT
NF1-RBX1	chr17	29422387	chr22	41349558	6	15	VVSRFDEQW	GTCAGCCGCTTCGACGAGCAGTGGAAT
NF1-RBX1	chr17	29422387	chr22	41349558	6	17	VVSRFDEQWNA	GTCAGCCGCTTCGACGAGCAGTGGAATGCAGTA
NF1-RBX1	chr17	29422387	chr22	41349558	7	15	VSRFDEQW	AGCCGCTTCGACGAGCAGTGGAAT
NF1-RBX1	chr17	29422387	chr22	41349558	7	17	VSRFDEQWNA	AGCCGCTTCGACGAGCAGTGGAATGCAGTA
NF1-RBX1	chr17	29422387	chr22	41349558	8	17	SRFDEQWNA	CGCTTCGACGAGCAGTGGAATGCAGTA
NF1-RBX1	chr17	29422387	chr22	41349558	8	18	SRFDEQWNAV	CGCTTCGACGAGCAGTGGAATGCAGTAGCC
NF1-RBX1	chr17	29422387	chr22	41349558	8	19	SRFDEQWNAVA	CGCTTCGACGAGCAGTGGAATGCAGTAGCCCTC
NF1-RBX1	chr17	29422387	chr22	41349558	9	18	RFDEQWNAV	TTCGACGAGCAGTGGAATGCAGTAGCC
NF1-RBX1	chr17	29422387	chr22	41349558	9	20	RFDEQWNAVAL	TTCGACGAGCAGTGGAATGCAGTAGCCCTCTGG

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene

Hchr

Hbp

Tchr

Tbp

Start in +/-13AA

End in +/-13AA

FusionNeoAntigen peptide

FusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of NF1-RBX1

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
OV	NF1-RBX1	chr17	29422387	ENST00000356175	chr22	41349558	ENST00000216225	TCGA-61-1738

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Potential target of CAR-T therapy development for NF1-RBX1

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to NF1-RBX1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to NF1-RBX1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	NF1	C0027831	Neurofibromatosis 1	44	CTD_human;GENOMICS_ENGLAND;UNIPROT
Hgene	NF1	C1708353	Hereditary Paraganglioma-Pheochromocytoma Syndrome	10	CLINGEN
Hgene	NF1	C0349639	Juvenile Myelomonocytic Leukemia	7	CTD_human;GENOMICS_ENGLAND;ORPHANET
Hgene	NF1	C2931482	Neurofibromatosis-Noonan syndrome	6	CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Hgene	NF1	C0553586	Cafe-au-lait macules with pulmonary stenosis	5	CTD_human;GENOMICS_ENGLAND;ORPHANET
Hgene	NF1	C0162678	Neurofibromatoses	3	CGI;CTD_human;GENOMICS_ENGLAND
Hgene	NF1	C0004114	Astrocytoma	2	CTD_human
Hgene	NF1	C0023467	Leukemia, Myelocytic, Acute	2	CTD_human
Hgene	NF1	C0025202	melanoma	2	CGI;CTD_human
Hgene	NF1	C0026998	Acute Myeloid Leukemia, M1	2	CTD_human
Hgene	NF1	C0205768	Subependymal Giant Cell Astrocytoma	2	CTD_human
Hgene	NF1	C0206727	Nerve Sheath Tumors	2	CTD_human
Hgene	NF1	C0280783	Juvenile Pilocytic Astrocytoma	2	CTD_human
Hgene	NF1	C0280785	Diffuse Astrocytoma	2	CTD_human
Hgene	NF1	C0334579	Anaplastic astrocytoma	2	CTD_human
Hgene	NF1	C0334580	Protoplasmic astrocytoma	2	CTD_human
Hgene	NF1	C0334581	Gemistocytic astrocytoma	2	CTD_human
Hgene	NF1	C0334582	Fibrillary Astrocytoma	2	CTD_human
Hgene	NF1	C0334583	Pilocytic Astrocytoma	2	CTD_human
Hgene	NF1	C0338070	Childhood Cerebral Astrocytoma	2	CTD_human
Hgene	NF1	C0547065	Mixed oligoastrocytoma	2	CTD_human
Hgene	NF1	C0750935	Cerebral Astrocytoma	2	CTD_human
Hgene	NF1	C0750936	Intracranial Astrocytoma	2	CTD_human
Hgene	NF1	C0751689	Peripheral Nerve Sheath Neoplasm	2	CTD_human
Hgene	NF1	C0751691	Perineurioma	2	CTD_human
Hgene	NF1	C1704230	Grade I Astrocytoma	2	CTD_human
Hgene	NF1	C1834235	NEUROFIBROMATOSIS, FAMILIAL SPINAL	2	CTD_human;GENOMICS_ENGLAND;UNIPROT
Hgene	NF1	C1879321	Acute Myeloid Leukemia (AML-M2)	2	CTD_human
Hgene	NF1	C0001430	Adenoma	1	CTD_human
Hgene	NF1	C0004352	Autistic Disorder	1	CTD_human
Hgene	NF1	C0016057	Fibrosarcoma	1	CTD_human
Hgene	NF1	C0017636	Glioblastoma	1	CTD_human
Hgene	NF1	C0017638	Glioma	1	CGI;CTD_human
Hgene	NF1	C0020796	Profound Mental Retardation	1	CTD_human
Hgene	NF1	C0023186	Learning Disorders	1	CTD_human
Hgene	NF1	C0023827	liposarcoma	1	CTD_human
Hgene	NF1	C0025363	Mental Retardation, Psychosocial	1	CTD_human
Hgene	NF1	C0026654	Moyamoya Disease	1	GENOMICS_ENGLAND
Hgene	NF1	C0027809	Neurilemmoma	1	CTD_human
Hgene	NF1	C0027830	neurofibroma	1	CTD_human
Hgene	NF1	C0027962	Melanocytic nevus	1	CTD_human
Hgene	NF1	C0028326	Noonan Syndrome	1	GENOMICS_ENGLAND
Hgene	NF1	C0031511	Pheochromocytoma	1	CTD_human
Hgene	NF1	C0035320	Retinal Neovascularization	1	CTD_human
Hgene	NF1	C0205646	Adenoma, Basal Cell	1	CTD_human
Hgene	NF1	C0205647	Follicular adenoma	1	CTD_human
Hgene	NF1	C0205648	Adenoma, Microcystic	1	CTD_human
Hgene	NF1	C0205649	Adenoma, Monomorphic	1	CTD_human
Hgene	NF1	C0205650	Papillary adenoma	1	CTD_human
Hgene	NF1	C0205651	Adenoma, Trabecular	1	CTD_human
Hgene	NF1	C0205824	Liposarcoma, Dedifferentiated	1	CTD_human
Hgene	NF1	C0205825	Liposarcoma, Pleomorphic	1	CTD_human
Hgene	NF1	C0205944	Sarcoma, Epithelioid	1	CTD_human
Hgene	NF1	C0205945	Sarcoma, Spindle Cell	1	CTD_human
Hgene	NF1	C0259783	mixed gliomas	1	CTD_human
Hgene	NF1	C0334588	Giant Cell Glioblastoma	1	CTD_human
Hgene	NF1	C0555198	Malignant Glioma	1	CTD_human
Hgene	NF1	C0751262	Adult Learning Disorders	1	CTD_human
Hgene	NF1	C0751263	Learning Disturbance	1	CTD_human
Hgene	NF1	C0751265	Learning Disabilities	1	CTD_human
Hgene	NF1	C0751374	Schwannomatosis, Plexiform	1	CTD_human
Hgene	NF1	C0917816	Mental deficiency	1	CTD_human
Hgene	NF1	C0917817	Neurofibromatosis 3	1	CTD_human
Hgene	NF1	C1257877	Pheochromocytoma, Extra-Adrenal	1	CTD_human
Hgene	NF1	C1261473	Sarcoma	1	CTD_human
Hgene	NF1	C1330966	Developmental Academic Disorder	1	CTD_human
Hgene	NF1	C1370889	Liposarcoma, well differentiated	1	CTD_human
Hgene	NF1	C1621958	Glioblastoma Multiforme	1	CTD_human
Hgene	NF1	C3150928	NF1 Microdeletion Syndrome	1	ORPHANET
Hgene	NF1	C3714756	Intellectual Disability	1	CTD_human

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)