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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:NF2-GRAP2

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: NF2-GRAP2
FusionPDB ID: 58724
FusionGDB2.0 ID: 58724
HgeneTgene
Gene symbol

NF2

GRAP2

Gene ID

4771

9402

Gene nameneurofibromin 2GRB2 related adaptor protein 2
SynonymsACN|BANF|SCHGADS|GRAP-2|GRB2L|GRBLG|GRID|GRPL|GrbX|Grf40|Mona|P38
Cytomap

22q12.2

22q13.1

Type of geneprotein-codingprotein-coding
Descriptionmerlinmoesin-ezrin-radixin likemoesin-ezrin-radixin-like proteinmoesin-ezrin-radizin-like proteinneurofibromin 2 (bilateral acoustic neuroma)schwannomerlinschwannominGRB2-related adapter protein 2GRB-2-like proteinGRB2-related protein with insert domainSH3-SH2-SH3 adapter MonaSH3-SH2-SH3 adaptor moleculeadapter protein GRIDgrf-40grf40 adapter proteingrowth factor receptor-binding proteingrowth factor receptor
Modification date2020032220200327
UniProtAcc

P35240

Main function of 5'-partner protein: FUNCTION: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. {ECO:0000269|PubMed:20159598, ECO:0000269|PubMed:20178741, ECO:0000269|PubMed:21167305}.

O75791

Main function of 5'-partner protein: FUNCTION: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
Ensembl transtripts involved in fusion geneENST idsENST00000338641, ENST00000361166, 
ENST00000361676, ENST00000397789, 
ENST00000403435, ENST00000403999, 
ENST00000413209, ENST00000334961, 
ENST00000347330, ENST00000353887, 
ENST00000361452, 		ENST00000478445, 
ENST00000399090, ENST00000407075, 
ENST00000540310, ENST00000544756, 
ENST00000344138, ENST00000543252, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score15 X 11 X 7=115512 X 12 X 7=1008
# samples 1814
** MAII scorelog2(18/1155*10)=-2.68182403997375
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(14/1008*10)=-2.84799690655495
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: NF2 [Title/Abstract] AND GRAP2 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: NF2 [Title/Abstract] AND GRAP2 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)NF2(30035201)-GRAP2(40351823), # samples:3
Anticipated loss of major functional domain due to fusion event.NF2-GRAP2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
NF2-GRAP2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
NF2-GRAP2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
NF2-GRAP2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneNF2

GO:0008285

negative regulation of cell proliferation

12444102|20178741

HgeneNF2

GO:0022408

negative regulation of cell-cell adhesion

17210637

HgeneNF2

GO:0042532

negative regulation of tyrosine phosphorylation of STAT protein

12444102

HgeneNF2

GO:0046426

negative regulation of JAK-STAT cascade

12444102



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:30035201/chr22:40351823)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across NF2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across GRAP2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000413209NF2chr2230035201+ENST00000543252GRAP2chr2240351823+15658063621564401
ENST00000413209NF2chr2230035201+ENST00000344138GRAP2chr2240351823+43588063621720452
ENST00000338641NF2chr2230035201+ENST00000543252GRAP2chr2240351823+15638043601562400
ENST00000338641NF2chr2230035201+ENST00000344138GRAP2chr2240351823+43568043601718452
ENST00000403435NF2chr2230035201+ENST00000543252GRAP2chr2240351823+15337743301532400
ENST00000403435NF2chr2230035201+ENST00000344138GRAP2chr2240351823+43267743301688452
ENST00000403999NF2chr2230035201+ENST00000543252GRAP2chr2240351823+14887292851487400
ENST00000403999NF2chr2230035201+ENST00000344138GRAP2chr2240351823+42817292851643452
ENST00000361166NF2chr2230035201+ENST00000543252GRAP2chr2240351823+112236301121373
ENST00000361166NF2chr2230035201+ENST00000344138GRAP2chr2240351823+391536301277425
ENST00000397789NF2chr2230035201+ENST00000543252GRAP2chr2240351823+112236301121373
ENST00000397789NF2chr2230035201+ENST00000344138GRAP2chr2240351823+391536301277425
ENST00000361676NF2chr2230035201+ENST00000543252GRAP2chr2240351823+9962370995331
ENST00000361676NF2chr2230035201+ENST00000344138GRAP2chr2240351823+378923701151383
ENST00000413209NF2chr2230035201+ENST00000543252GRAP2chr2240351822+15658063621564401
ENST00000413209NF2chr2230035201+ENST00000344138GRAP2chr2240351822+43588063621720452
ENST00000338641NF2chr2230035201+ENST00000543252GRAP2chr2240351822+15638043601562400
ENST00000338641NF2chr2230035201+ENST00000344138GRAP2chr2240351822+43568043601718452
ENST00000403435NF2chr2230035201+ENST00000543252GRAP2chr2240351822+15337743301532400
ENST00000403435NF2chr2230035201+ENST00000344138GRAP2chr2240351822+43267743301688452
ENST00000403999NF2chr2230035201+ENST00000543252GRAP2chr2240351822+14887292851487400
ENST00000403999NF2chr2230035201+ENST00000344138GRAP2chr2240351822+42817292851643452
ENST00000361166NF2chr2230035201+ENST00000543252GRAP2chr2240351822+112236301121373
ENST00000361166NF2chr2230035201+ENST00000344138GRAP2chr2240351822+391536301277425
ENST00000397789NF2chr2230035201+ENST00000543252GRAP2chr2240351822+112236301121373
ENST00000397789NF2chr2230035201+ENST00000344138GRAP2chr2240351822+391536301277425
ENST00000361676NF2chr2230035201+ENST00000543252GRAP2chr2240351822+9962370995331
ENST00000361676NF2chr2230035201+ENST00000344138GRAP2chr2240351822+378923701151383

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000413209ENST00000543252NF2chr2230035201+GRAP2chr2240351823+0.0162184690.9837816
ENST00000413209ENST00000344138NF2chr2230035201+GRAP2chr2240351823+0.0042398790.99576014
ENST00000338641ENST00000543252NF2chr2230035201+GRAP2chr2240351823+0.016278240.9837218
ENST00000338641ENST00000344138NF2chr2230035201+GRAP2chr2240351823+0.0042355860.9957644
ENST00000403435ENST00000543252NF2chr2230035201+GRAP2chr2240351823+0.0175617990.98243827
ENST00000403435ENST00000344138NF2chr2230035201+GRAP2chr2240351823+0.0044149750.99558496
ENST00000403999ENST00000543252NF2chr2230035201+GRAP2chr2240351823+0.0180451160.9819549
ENST00000403999ENST00000344138NF2chr2230035201+GRAP2chr2240351823+0.004590960.9954091
ENST00000361166ENST00000543252NF2chr2230035201+GRAP2chr2240351823+0.0158489890.984151
ENST00000361166ENST00000344138NF2chr2230035201+GRAP2chr2240351823+0.0041367220.9958633
ENST00000397789ENST00000543252NF2chr2230035201+GRAP2chr2240351823+0.0158489890.984151
ENST00000397789ENST00000344138NF2chr2230035201+GRAP2chr2240351823+0.0041367220.9958633
ENST00000361676ENST00000543252NF2chr2230035201+GRAP2chr2240351823+0.0069926680.9930073
ENST00000361676ENST00000344138NF2chr2230035201+GRAP2chr2240351823+0.0033031120.99669695
ENST00000413209ENST00000543252NF2chr2230035201+GRAP2chr2240351822+0.0162184690.9837816
ENST00000413209ENST00000344138NF2chr2230035201+GRAP2chr2240351822+0.0042398790.99576014
ENST00000338641ENST00000543252NF2chr2230035201+GRAP2chr2240351822+0.016278240.9837218
ENST00000338641ENST00000344138NF2chr2230035201+GRAP2chr2240351822+0.0042355860.9957644
ENST00000403435ENST00000543252NF2chr2230035201+GRAP2chr2240351822+0.0175617990.98243827
ENST00000403435ENST00000344138NF2chr2230035201+GRAP2chr2240351822+0.0044149750.99558496
ENST00000403999ENST00000543252NF2chr2230035201+GRAP2chr2240351822+0.0180451160.9819549
ENST00000403999ENST00000344138NF2chr2230035201+GRAP2chr2240351822+0.004590960.9954091
ENST00000361166ENST00000543252NF2chr2230035201+GRAP2chr2240351822+0.0158489890.984151
ENST00000361166ENST00000344138NF2chr2230035201+GRAP2chr2240351822+0.0041367220.9958633
ENST00000397789ENST00000543252NF2chr2230035201+GRAP2chr2240351822+0.0158489890.984151
ENST00000397789ENST00000344138NF2chr2230035201+GRAP2chr2240351822+0.0041367220.9958633
ENST00000361676ENST00000543252NF2chr2230035201+GRAP2chr2240351822+0.0069926680.9930073
ENST00000361676ENST00000344138NF2chr2230035201+GRAP2chr2240351822+0.0033031120.99669695

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for NF2-GRAP2

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
NF2chr2230035201GRAP2chr224035182223779LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351822363121LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351822729148LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351822774148LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351822804148LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351822806148LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr224035182323779LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351823363121LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351823729148LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351823774148LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351823804148LVQEITQHLFFLQILSNQEEWFKAEL
NF2chr2230035201GRAP2chr2240351823806148LVQEITQHLFFLQILSNQEEWFKAEL

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Potential FusionNeoAntigen Information of NF2-GRAP2 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
NF2-GRAP2_30035201_40351822.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:24QHLFFLQIL0.99830.7098615
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:01QHLFFLQIL0.99820.9514615
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:06QHLFFLQIL0.99720.8093615
NF2-GRAP2chr2230035201chr2240351822804HLA-B38:01QHLFFLQIL0.99680.9888615
NF2-GRAP2chr2230035201chr2240351822804HLA-B38:02QHLFFLQIL0.99670.9884615
NF2-GRAP2chr2230035201chr2240351822804HLA-B14:01QHLFFLQIL0.99160.837615
NF2-GRAP2chr2230035201chr2240351822804HLA-B14:02QHLFFLQIL0.99160.837615
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:10QHLFFLQIL0.98450.507615
NF2-GRAP2chr2230035201chr2240351822804HLA-B58:01QILSNQEEW0.97210.98641221
NF2-GRAP2chr2230035201chr2240351822804HLA-B57:01QILSNQEEW0.92630.99271221
NF2-GRAP2chr2230035201chr2240351822804HLA-B58:02QILSNQEEW0.90160.98321221
NF2-GRAP2chr2230035201chr2240351822804HLA-B13:02TQHLFFLQI0.88730.6874514
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:18QHLFFLQIL0.85130.7277615
NF2-GRAP2chr2230035201chr2240351822804HLA-B57:03QILSNQEEW0.78390.99491221
NF2-GRAP2chr2230035201chr2240351822804HLA-B13:01TQHLFFLQI0.74750.874514
NF2-GRAP2chr2230035201chr2240351822804HLA-A32:13QILSNQEEW0.69040.97451221
NF2-GRAP2chr2230035201chr2240351822804HLA-B53:01QILSNQEEW0.60450.56231221
NF2-GRAP2chr2230035201chr2240351822804HLA-B52:01TQHLFFLQI0.36150.8317514
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:09QHLFFLQIL0.99830.8132615
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:12QHLFFLQIL0.99760.955615
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:05QHLFFLQIL0.99610.9413615
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:04TQHLFFLQI0.86880.8003514
NF2-GRAP2chr2230035201chr2240351822804HLA-B14:03QHLFFLQIL0.39350.8455615
NF2-GRAP2chr2230035201chr2240351822804HLA-B51:07TQHLFFLQI0.27750.7868514
NF2-GRAP2chr2230035201chr2240351822804HLA-B44:08QILSNQEEW0.16320.53211221
NF2-GRAP2chr2230035201chr2240351822804HLA-B44:08LQILSNQEEW0.75110.69281121
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:31QHLFFLQIL0.99820.9525615
NF2-GRAP2chr2230035201chr2240351822804HLA-B38:05QHLFFLQIL0.99680.9888615
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:09QHLFFLQIL0.98440.7862615
NF2-GRAP2chr2230035201chr2240351822804HLA-B57:04QILSNQEEW0.97860.80011221
NF2-GRAP2chr2230035201chr2240351822804HLA-B57:10QILSNQEEW0.92630.99271221
NF2-GRAP2chr2230035201chr2240351822804HLA-B58:06QILSNQEEW0.89120.96611221
NF2-GRAP2chr2230035201chr2240351822804HLA-A32:01QILSNQEEW0.8870.97131221
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:73TQHLFFLQI0.88070.8404514
NF2-GRAP2chr2230035201chr2240351822804HLA-B57:02QILSNQEEW0.87850.95511221
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:13QILSNQEEW0.86430.7761221
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:24QILSNQEEW0.74960.92911221
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:11QHLFFLQIL0.67220.9426615
NF2-GRAP2chr2230035201chr2240351822804HLA-B39:02TQHLFFLQI0.52490.9049514
NF2-GRAP2chr2230035201chr2240351822804HLA-B53:02QILSNQEEW0.36270.59471221
NF2-GRAP2chr2230035201chr2240351822804HLA-A25:01QILSNQEEW0.26170.91211221
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:24LQILSNQEEW0.99750.96641121
NF2-GRAP2chr2230035201chr2240351822804HLA-B15:13LQILSNQEEW0.78830.7971121

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Potential FusionNeoAntigen Information of NF2-GRAP2 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of NF2-GRAP2

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
7298QHLFFLQILSNQEENF2GRAP2chr2230035201chr2240351822804

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of NF2-GRAP2

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN7298QHLFFLQILSNQEE-7.15543-7.26883
HLA-B14:023BVN7298QHLFFLQILSNQEE-4.77435-5.80965
HLA-B52:013W397298QHLFFLQILSNQEE-6.80875-6.92215
HLA-B52:013W397298QHLFFLQILSNQEE-4.20386-5.23916
HLA-A11:014UQ27298QHLFFLQILSNQEE-7.5194-8.5547
HLA-A11:014UQ27298QHLFFLQILSNQEE-6.9601-7.0735
HLA-A24:025HGA7298QHLFFLQILSNQEE-7.52403-7.63743
HLA-A24:025HGA7298QHLFFLQILSNQEE-5.82433-6.85963
HLA-B27:056PYJ7298QHLFFLQILSNQEE-3.28285-4.31815
HLA-B44:053DX87298QHLFFLQILSNQEE-5.91172-6.94702
HLA-B44:053DX87298QHLFFLQILSNQEE-4.24346-4.35686

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Vaccine Design for the FusionNeoAntigens of NF2-GRAP2

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
NF2-GRAP2chr2230035201chr22403518221121LQILSNQEEWTTACAGATTTTAAGTAACCAAGAGGAGTGG
NF2-GRAP2chr2230035201chr22403518221221QILSNQEEWCAGATTTTAAGTAACCAAGAGGAGTGG
NF2-GRAP2chr2230035201chr2240351822514TQHLFFLQIACACAACATTTATTCTTCTTACAGATT
NF2-GRAP2chr2230035201chr2240351822615QHLFFLQILCAACATTTATTCTTCTTACAGATTTTA

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of NF2-GRAP2

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BRCANF2-GRAP2chr2230035201ENST00000338641chr2240351822ENST00000344138TCGA-A2-A0SV

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Potential target of CAR-T therapy development for NF2-GRAP2

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to NF2-GRAP2

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to NF2-GRAP2

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource