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Fusion Protein:NR6A1-DEAF1 |
Fusion Gene and Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: NR6A1-DEAF1 | FusionPDB ID: 60188 | FusionGDB2.0 ID: 60188 | Hgene | Tgene | Gene symbol | NR6A1 | DEAF1 | Gene ID | 2649 | 10522 |
Gene name | nuclear receptor subfamily 6 group A member 1 | DEAF1 transcription factor | |
Synonyms | CT150|GCNF|GCNF1|NR61|RTR|hGCNF|hRTR | MRD24|NUDR|SPN|ZMYND5 | |
Cytomap | 9q33.3 | 11p15.5 | |
Type of gene | protein-coding | protein-coding | |
Description | nuclear receptor subfamily 6 group A member 1germ cell nuclear factor variant 1retinoic acid receptor-related testis-associated receptorretinoid receptor-related testis-specific receptor | deformed epidermal autoregulatory factor 1 homolognuclear DEAF-1-related transcriptional regulatorsuppressinzinc finger MYND domain-containing protein 5 | |
Modification date | 20200313 | 20200313 | |
UniProtAcc | Q15406 Main function of 5'-partner protein: FUNCTION: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis (By similarity). {ECO:0000250}. | O75398 Main function of 5'-partner protein: FUNCTION: Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm. Transcriptional activator of EIF4G3. {ECO:0000269|PubMed:10521432, ECO:0000269|PubMed:11427895, ECO:0000269|PubMed:11705868, ECO:0000269|PubMed:18826651, ECO:0000269|PubMed:19668219, ECO:0000269|PubMed:24726472}. | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000344523, ENST00000373584, ENST00000416460, ENST00000487099, | ENST00000525904, ENST00000338675, ENST00000382409, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 4 X 5 X 5=100 | 9 X 5 X 7=315 |
# samples | 8 | 11 | |
** MAII score | log2(8/100*10)=-0.321928094887362 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(11/315*10)=-1.51784830486262 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Fusion gene context | PubMed: NR6A1 [Title/Abstract] AND DEAF1 [Title/Abstract] AND fusion [Title/Abstract] | ||
Fusion neoantigen context | PubMed: NR6A1 [Title/Abstract] AND DEAF1 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | NR6A1(127533299)-DEAF1(654051), # samples:2 | ||
Anticipated loss of major functional domain due to fusion event. | NR6A1-DEAF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NR6A1-DEAF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NR6A1-DEAF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NR6A1-DEAF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
Tgene | DEAF1 | GO:0000122 | negative regulation of transcription by RNA polymerase II | 24726472 |
Tgene | DEAF1 | GO:0033599 | regulation of mammary gland epithelial cell proliferation | 18826651 |
Tgene | DEAF1 | GO:0045892 | negative regulation of transcription, DNA-templated | 24726472 |
Tgene | DEAF1 | GO:0045893 | positive regulation of transcription, DNA-templated | 24726472 |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:127533299/chr11:654051) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000487099 | NR6A1 | chr9 | 127533299 | - | ENST00000338675 | DEAF1 | chr11 | 654051 | - | 770 | 258 | 255 | 1 | 85 |
ENST00000487099 | NR6A1 | chr9 | 127533299 | - | ENST00000382409 | DEAF1 | chr11 | 654051 | - | 770 | 258 | 255 | 1 | 85 |
ENST00000373584 | NR6A1 | chr9 | 127533299 | - | ENST00000338675 | DEAF1 | chr11 | 654051 | - | 790 | 278 | 275 | 0 | 92 |
ENST00000373584 | NR6A1 | chr9 | 127533299 | - | ENST00000382409 | DEAF1 | chr11 | 654051 | - | 790 | 278 | 275 | 0 | 92 |
ENST00000416460 | NR6A1 | chr9 | 127533299 | - | ENST00000338675 | DEAF1 | chr11 | 654051 | - | 803 | 291 | 288 | 1 | 96 |
ENST00000416460 | NR6A1 | chr9 | 127533299 | - | ENST00000382409 | DEAF1 | chr11 | 654051 | - | 803 | 291 | 288 | 1 | 96 |
ENST00000344523 | NR6A1 | chr9 | 127533299 | - | ENST00000338675 | DEAF1 | chr11 | 654051 | - | 780 | 268 | 265 | 2 | 88 |
ENST00000344523 | NR6A1 | chr9 | 127533299 | - | ENST00000382409 | DEAF1 | chr11 | 654051 | - | 780 | 268 | 265 | 2 | 88 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000487099 | ENST00000338675 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.23508762 | 0.76491237 |
ENST00000487099 | ENST00000382409 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.23508762 | 0.76491237 |
ENST00000373584 | ENST00000338675 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.16021211 | 0.83978784 |
ENST00000373584 | ENST00000382409 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.16021211 | 0.83978784 |
ENST00000416460 | ENST00000338675 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.152966 | 0.847034 |
ENST00000416460 | ENST00000382409 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.152966 | 0.847034 |
ENST00000344523 | ENST00000338675 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.26967523 | 0.73032475 |
ENST00000344523 | ENST00000382409 | NR6A1 | chr9 | 127533299 | - | DEAF1 | chr11 | 654051 | - | 0.26967523 | 0.73032475 |
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Get the fusion protein sequences from here. |
Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for NR6A1-DEAF1 |
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Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
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Potential FusionNeoAntigen Information of NR6A1-DEAF1 in HLA I |
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![]() * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Potential FusionNeoAntigen Information of NR6A1-DEAF1 in HLA II |
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![]() * We used NetMHCIIpan v4.1 (%rank<0.5). |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of NR6A1-DEAF1 |
![]() * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of NR6A1-DEAF1 |
![]() * We used Glide to predict the interaction between HLAs and neoantigens. |
HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
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Vaccine Design for the FusionNeoAntigens of NR6A1-DEAF1 |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of NR6A1-DEAF1 |
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Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
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Potential target of CAR-T therapy development for NR6A1-DEAF1 |
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![]() * Minus value of BPloci means that the break point is located before the CDS. |
- In-frame and retained 'Transmembrane'. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
![]() * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to NR6A1-DEAF1 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to NR6A1-DEAF1 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |