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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:PARD3-ZNF521

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: PARD3-ZNF521
FusionPDB ID: 62723
FusionGDB2.0 ID: 62723
HgeneTgene
Gene symbol

PARD3

ZNF521

Gene ID

56288

25925

Gene namepar-3 family cell polarity regulatorzinc finger protein 521
SynonymsASIP|Baz|PAR3|PAR3alpha|PARD-3|PARD3A|PPP1R118|SE2-5L16|SE2-5LT1|SE2-5T2EHZF|Evi3
Cytomap

10p11.22-p11.21

18q11.2

Type of geneprotein-codingprotein-coding
Descriptionpartitioning defective 3 homologCTCL tumor antigen se2-5PAR3-alphaatypical PKC isotype-specific interacting proteinbazookapar-3 family cell polarity regulator alphapar-3 partitioning defective 3 homologprotein phosphatase 1, regulatory subunit 118zinc finger protein 521LYST-interacting protein 3early hematopoietic zinc finger protein
Modification date2020032720200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST idsENST00000340077, ENST00000346874, 
ENST00000350537, ENST00000374773, 
ENST00000374776, ENST00000374788, 
ENST00000374789, ENST00000374790, 
ENST00000374794, ENST00000545260, 
ENST00000545693, ENST00000374768, 
ENST00000466092, ENST00000544292, 
ENST00000579111, ENST00000361524, 
ENST00000538137, ENST00000584787, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score33 X 20 X 14=924013 X 10 X 9=1170
# samples 4915
** MAII scorelog2(49/9240*10)=-4.23703919730085
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(15/1170*10)=-2.96347412397489
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: PARD3 [Title/Abstract] AND ZNF521 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: PARD3 [Title/Abstract] AND ZNF521 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)PARD3(34805907)-ZNF521(22672045), # samples:3
Anticipated loss of major functional domain due to fusion event.PARD3-ZNF521 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
PARD3-ZNF521 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
PARD3-ZNF521 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
PARD3-ZNF521 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:34805907/chr18:22672045)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across PARD3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across ZNF521 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000545260PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17977331981010270
ENST00000545260PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12987331981010270
ENST00000545260PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11307331981010270
ENST00000545693PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17977331981010270
ENST00000545693PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12987331981010270
ENST00000545693PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11307331981010270
ENST00000350537PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000350537PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000350537PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000346874PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000346874PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000346874PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374789PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374789PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374789PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374790PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374790PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374790PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374794PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374794PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374794PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374788PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374788PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374788PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374776PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374776PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374776PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000340077PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17977331981010270
ENST00000340077PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12987331981010270
ENST00000340077PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11307331981010270
ENST00000374773PARD3chr1034805907-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374773PARD3chr1034805907-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374773PARD3chr1034805907-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000545260PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17977331981010270
ENST00000545260PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12987331981010270
ENST00000545260PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11307331981010270
ENST00000545693PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17977331981010270
ENST00000545693PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12987331981010270
ENST00000545693PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11307331981010270
ENST00000350537PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000350537PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000350537PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000346874PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000346874PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000346874PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374789PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374789PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374789PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374790PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374790PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374790PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374794PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374794PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374794PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374788PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374788PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374788PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000374776PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374776PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374776PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270
ENST00000340077PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17977331981010270
ENST00000340077PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12987331981010270
ENST00000340077PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11307331981010270
ENST00000374773PARD3chr1034805906-ENST00000361524ZNF521chr1822672045-17937291941006270
ENST00000374773PARD3chr1034805906-ENST00000584787ZNF521chr1822672045-12947291941006270
ENST00000374773PARD3chr1034805906-ENST00000538137ZNF521chr1822672045-11267291941006270

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000545260ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0015386010.99846137
ENST00000545260ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035896330.9964103
ENST00000545260ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0041062360.9958937
ENST00000545693ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0015386010.99846137
ENST00000545693ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035896330.9964103
ENST00000545693ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0041062360.9958937
ENST00000350537ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000350537ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000350537ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000346874ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000346874ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000346874ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374789ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374789ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374789ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374790ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374790ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374790ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374794ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374794ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374794ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374788ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374788ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374788ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374776ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374776ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374776ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000340077ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0015386010.99846137
ENST00000340077ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035896330.9964103
ENST00000340077ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0041062360.9958937
ENST00000374773ENST00000361524PARD3chr1034805907-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374773ENST00000584787PARD3chr1034805907-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374773ENST00000538137PARD3chr1034805907-ZNF521chr1822672045-0.0039752080.9960247
ENST00000545260ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0015386010.99846137
ENST00000545260ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035896330.9964103
ENST00000545260ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0041062360.9958937
ENST00000545693ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0015386010.99846137
ENST00000545693ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035896330.9964103
ENST00000545693ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0041062360.9958937
ENST00000350537ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000350537ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000350537ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247
ENST00000346874ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000346874ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000346874ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374789ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374789ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374789ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374790ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374790ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374790ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374794ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374794ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374794ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374788ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374788ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374788ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247
ENST00000374776ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374776ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374776ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247
ENST00000340077ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0015386010.99846137
ENST00000340077ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035896330.9964103
ENST00000340077ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0041062360.9958937
ENST00000374773ENST00000361524PARD3chr1034805906-ZNF521chr1822672045-0.0016624360.9983375
ENST00000374773ENST00000584787PARD3chr1034805906-ZNF521chr1822672045-0.0035025850.99649745
ENST00000374773ENST00000538137PARD3chr1034805906-ZNF521chr1822672045-0.0039752080.9960247

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for PARD3-ZNF521

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
PARD3chr1034805906ZNF521chr1822672045729179SEIEVTPSVLRANEGLNHECKLCSQT
PARD3chr1034805906ZNF521chr1822672045733179SEIEVTPSVLRANEGLNHECKLCSQT
PARD3chr1034805907ZNF521chr1822672045729179SEIEVTPSVLRANEGLNHECKLCSQT
PARD3chr1034805907ZNF521chr1822672045733179SEIEVTPSVLRANEGLNHECKLCSQT

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Potential FusionNeoAntigen Information of PARD3-ZNF521 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Potential FusionNeoAntigen Information of PARD3-ZNF521 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
PARD3-ZNF521_34805906_22672045.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
PARD3-ZNF521chr1034805906chr1822672045733DRB1-1525SEIEVTPSVLRANEG015
PARD3-ZNF521chr1034805906chr1822672045733DRB3-0201PSVLRANEGLNHECK621
PARD3-ZNF521chr1034805906chr1822672045733DRB3-0201VTPSVLRANEGLNHE419
PARD3-ZNF521chr1034805906chr1822672045733DRB3-0204PSVLRANEGLNHECK621
PARD3-ZNF521chr1034805906chr1822672045733DRB3-0204VTPSVLRANEGLNHE419
PARD3-ZNF521chr1034805906chr1822672045733DRB3-0214PSVLRANEGLNHECK621
PARD3-ZNF521chr1034805906chr1822672045733DRB3-0224PSVLRANEGLNHECK621
PARD3-ZNF521chr1034805906chr1822672045733DRB3-0224VTPSVLRANEGLNHE419
PARD3-ZNF521chr1034805906chr1822672045733DRB5-0106SEIEVTPSVLRANEG015
PARD3-ZNF521chr1034805906chr1822672045733DRB5-0111SEIEVTPSVLRANEG015
PARD3-ZNF521chr1034805906chr1822672045733DRB5-0202SEIEVTPSVLRANEG015
PARD3-ZNF521chr1034805906chr1822672045733DRB5-0205SEIEVTPSVLRANEG015

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Fusion breakpoint peptide structures of PARD3-ZNF521

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of PARD3-ZNF521

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score

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Vaccine Design for the FusionNeoAntigens of PARD3-ZNF521

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
PARD3-ZNF521chr1034805906chr1822672045015SEIEVTPSVLRANEGCAAGTGAAATTGAGGTCACACCTTCAGTCCTTCGAGCAAATGAAG
PARD3-ZNF521chr1034805906chr1822672045419VTPSVLRANEGLNHEAGGTCACACCTTCAGTCCTTCGAGCAAATGAAGGACTGAACCATG
PARD3-ZNF521chr1034805906chr1822672045621PSVLRANEGLNHECKCACCTTCAGTCCTTCGAGCAAATGAAGGACTGAACCATGAATGCA

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Information of the samples that have these potential fusion neoantigens of PARD3-ZNF521

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample

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Potential target of CAR-T therapy development for PARD3-ZNF521

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to PARD3-ZNF521

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to PARD3-ZNF521

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource