Home

Download

Statistics

Examples

Help

Contact

Fusion Protein:PLEKHB2-BIRC6

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: PLEKHB2-BIRC6
	FusionPDB ID: 66241
	FusionGDB2.0 ID: 66241
		Hgene	Tgene
	Gene symbol	PLEKHB2	BIRC6
	Gene ID	55041	57448
	Gene name	pleckstrin homology domain containing B2	baculoviral IAP repeat containing 6
	Synonyms	EVT2	APOLLON\|BRUCE
	Cytomap	2q21.1	2p22.3
	Type of gene	protein-coding	protein-coding
	Description	pleckstrin homology domain-containing family B member 2PH domain-containing family B member 2evectin-2pleckstrin homology domain containing, family B (evectins) member 2	baculoviral IAP repeat-containing protein 6BIR repeat-containing ubiquitin-conjugating enzymeRING-type E3 ubiquitin transferase BIRC6ubiquitin-conjugating BIR-domain enzyme apollon
	Modification date	20200313	20200313
	UniProtAcc	.	Q9NR09 Main function of 5'-partner protein: FUNCTION: Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. {ECO:0000269\|PubMed:14765125, ECO:0000269\|PubMed:15200957, ECO:0000269\|PubMed:18329369}.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000439822, ENST00000234115, ENST00000403716, ENST00000409158, ENST00000409279, ENST00000409612, ENST00000538982, ENST00000303908, ENST00000404460, ENST00000438882, ENST00000487980,	ENST00000421745,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	4 X 2 X 4=32	23 X 19 X 14=6118
	# samples	5	29
	** MAII score	log2(5/32*10)=0.643856189774725 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0	log2(29/6118*10)=-4.39893530154327 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: PLEKHB2 [Title/Abstract] AND BIRC6 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: PLEKHB2 [Title/Abstract] AND BIRC6 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	PLEKHB2(131897848)-BIRC6(32715108), # samples:1
Anticipated loss of major functional domain due to fusion event.	PLEKHB2-BIRC6 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. PLEKHB2-BIRC6 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. PLEKHB2-BIRC6 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. PLEKHB2-BIRC6 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. PLEKHB2-BIRC6 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. PLEKHB2-BIRC6 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:131897848/chr2:32715108)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across PLEKHB2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across BIRC6 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000409158	PLEKHB2	chr2	131897848	+	ENST00000421745	BIRC6	chr2	32715108	+	8590	1116	347	7594	2415
ENST00000403716	PLEKHB2	chr2	131897848	+	ENST00000421745	BIRC6	chr2	32715108	+	8566	1092	347	7570	2407
ENST00000234115	PLEKHB2	chr2	131897848	+	ENST00000421745	BIRC6	chr2	32715108	+	8466	992	250	7470	2406
ENST00000538982	PLEKHB2	chr2	131897848	+	ENST00000421745	BIRC6	chr2	32715108	+	8030	556	0	7034	2344
ENST00000409612	PLEKHB2	chr2	131897848	+	ENST00000421745	BIRC6	chr2	32715108	+	8523	1049	487	7527	2346
ENST00000409279	PLEKHB2	chr2	131897848	+	ENST00000421745	BIRC6	chr2	32715108	+	8801	1327	672	7805	2377

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000409158	ENST00000421745	PLEKHB2	chr2	131897848	+	BIRC6	chr2	32715108	+	0.001094911	0.99890506
ENST00000403716	ENST00000421745	PLEKHB2	chr2	131897848	+	BIRC6	chr2	32715108	+	0.001079707	0.9989203
ENST00000234115	ENST00000421745	PLEKHB2	chr2	131897848	+	BIRC6	chr2	32715108	+	0.00098364	0.9990164
ENST00000538982	ENST00000421745	PLEKHB2	chr2	131897848	+	BIRC6	chr2	32715108	+	0.000767355	0.99923265
ENST00000409612	ENST00000421745	PLEKHB2	chr2	131897848	+	BIRC6	chr2	32715108	+	0.00098813	0.9990119
ENST00000409279	ENST00000421745	PLEKHB2	chr2	131897848	+	BIRC6	chr2	32715108	+	0.001143551	0.9988564

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for PLEKHB2-BIRC6

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
PLEKHB2	chr2	131897848	BIRC6	chr2	32715108	1049	187	GQAYAVPYQYPYAASITSFLTVLAWY
PLEKHB2	chr2	131897848	BIRC6	chr2	32715108	1092	248	GQAYAVPYQYPYAASITSFLTVLAWY
PLEKHB2	chr2	131897848	BIRC6	chr2	32715108	1116	256	GQAYAVPYQYPYAASITSFLTVLAWY
PLEKHB2	chr2	131897848	BIRC6	chr2	32715108	1327	218	GQAYAVPYQYPYAASITSFLTVLAWY
PLEKHB2	chr2	131897848	BIRC6	chr2	32715108	556	185	GQAYAVPYQYPYAASITSFLTVLAWY
PLEKHB2	chr2	131897848	BIRC6	chr2	32715108	992	247	GQAYAVPYQYPYAASITSFLTVLAWY

Top

Potential FusionNeoAntigen Information of PLEKHB2-BIRC6 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

PLEKHB2-BIRC6_131897848_32715108.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:08	YAASITSF	0.9922	0.8473	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:01	YAASITSF	0.9853	0.7909	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B52:01	QYPYAASI	0.964	0.9426	8	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:06	YQYPYAASI	0.9973	0.7934	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B48:01	YQYPYAASI	0.9939	0.5055	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B14:01	YQYPYAASI	0.993	0.8037	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B14:02	YQYPYAASI	0.993	0.8037	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:01	YQYPYAASI	0.992	0.9337	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:03	YAASITSFL	0.9912	0.8861	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:21	YQYPYAASI	0.9906	0.5454	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:04	YQYPYAASI	0.9851	0.5359	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:13	YQYPYAASI	0.9798	0.9299	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B13:02	YQYPYAASI	0.9794	0.7083	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:01	YAASITSFL	0.9794	0.872	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:03	YPYAASITS	0.9747	0.6932	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:21	YAASITSFL	0.97	0.6495	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:01	YPYAASITS	0.9693	0.6562	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:03	VPYQYPYAA	0.9667	0.6243	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:05	YAASITSFL	0.9656	0.5644	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:02	YAASITSFL	0.9559	0.9458	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:04	YAASITSFL	0.9559	0.9458	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:16	YAASITSFL	0.9495	0.7002	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:04	VPYQYPYAA	0.9424	0.8421	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:02	VPYQYPYAA	0.9424	0.8421	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B38:02	YQYPYAASI	0.9421	0.9566	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:02	YPYAASITS	0.9417	0.7775	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:04	YPYAASITS	0.9417	0.7775	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B13:01	YQYPYAASI	0.9408	0.9605	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B38:01	YQYPYAASI	0.9368	0.9577	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:06	VPYQYPYAA	0.9239	0.6671	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:18	YQYPYAASI	0.9228	0.7495	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:03	YQYPYAASI	0.9125	0.8033	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:13	YQYPYAASI	0.9112	0.5372	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:01	VPYQYPYAA	0.9024	0.6668	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:10	YQYPYAASI	0.8733	0.586	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:25	YQYPYAASI	0.8318	0.9336	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B47:01	YQYPYAASI	0.8305	0.5914	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A32:13	YQYPYAASI	0.8091	0.9645	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B08:09	VPYQYPYAA	0.7712	0.5718	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:37	YQYPYAASI	0.6964	0.6045	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B08:09	YQYPYAASI	0.5741	0.589	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B52:01	YQYPYAASI	0.5062	0.9838	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:01	YPYAASITSF	0.9994	0.6106	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:08	YPYAASITSF	0.9987	0.6762	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:03	YPYAASITSF	0.9976	0.6745	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:02	YPYAASITSF	0.9974	0.7896	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:04	YPYAASITSF	0.9881	0.7656	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:02	YPYAASITSF	0.9881	0.7656	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:03	YPYAASITSFL	0.9993	0.7272	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:01	YPYAASITSFL	0.999	0.6845	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:04	YPYAASITSFL	0.997	0.8134	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:02	YPYAASITSFL	0.997	0.8134	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B52:01	VPYQYPYAASI	0.9933	0.7706	5	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:19	YAASITSF	0.9999	0.9877	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:08	YAASITSF	0.9999	0.8832	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:04	YAASITSF	0.9995	0.8974	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:07	YAASITSF	0.9994	0.9845	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:12	YAASITSF	0.9991	0.9242	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:21	YAASITSF	0.999	0.8665	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:06	YAASITSF	0.9975	0.8359	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:03	YAASITSF	0.9965	0.9937	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:13	YAASITSF	0.9961	0.9605	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:04	YAASITSF	0.9961	0.9605	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:04	YAASITSF	0.996	0.9943	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:14	YAASITSF	0.9923	0.9777	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:03	YAASITSF	0.9299	0.9749	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:19	YAASITSFL	0.9997	0.9898	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:08	YAASITSFL	0.9995	0.9029	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:07	YAASITSFL	0.9993	0.9876	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:04	YQYPYAASI	0.9992	0.996	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:03	YQYPYAASI	0.9992	0.9957	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:06	YAASITSFL	0.9991	0.9297	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:04	YAASITSFL	0.999	0.9171	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:12	YQYPYAASI	0.9984	0.9496	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:06	YAASITSFL	0.9979	0.8616	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:13	YAASITSFL	0.9954	0.9707	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:04	YAASITSFL	0.9954	0.9707	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:04	YAASITSFL	0.9944	0.9963	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:03	YAASITSFL	0.9943	0.9957	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:12	YAASITSFL	0.994	0.9408	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:12	YQYPYAASI	0.9927	0.9342	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:09	YQYPYAASI	0.992	0.6993	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:16	YAASITSFL	0.9911	0.9714	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:04	YQYPYAASI	0.9891	0.9439	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:14	YAASITSFL	0.9889	0.8181	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:06	YQYPYAASI	0.9889	0.9513	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:04	YQYPYAASI	0.9888	0.9191	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:06	YQYPYAASI	0.9881	0.9195	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:03	YAASITSFL	0.9868	0.9843	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B51:07	VPYQYPYAA	0.9867	0.8734	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:14	YQYPYAASI	0.9861	0.6882	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:14	YAASITSFL	0.9852	0.9828	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:10	YAASITSFL	0.9783	0.7069	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C05:09	YAASITSFL	0.9775	0.9316	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:07	YQYPYAASI	0.9768	0.765	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:07	YAASITSFL	0.9755	0.7213	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:07	YQYPYAASI	0.9729	0.9831	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:13	YQYPYAASI	0.9675	0.9814	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:04	YQYPYAASI	0.9675	0.9814	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:03	YQYPYAASI	0.9659	0.9823	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:05	YQYPYAASI	0.9636	0.9158	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:13	YAASITSFL	0.9625	0.91	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:19	YQYPYAASI	0.9604	0.9905	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:05	YAASITSFL	0.9573	0.968	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:12	YAASITSFL	0.9559	0.9458	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:29	YAASITSFL	0.9546	0.9539	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:05	YQYPYAASI	0.954	0.9778	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:08	YQYPYAASI	0.9494	0.7816	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:16	YQYPYAASI	0.9443	0.9809	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:12	VPYQYPYAA	0.9424	0.8421	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:12	YPYAASITS	0.9417	0.7775	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:27	YAASITSFL	0.938	0.9624	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:06	YAASITSFL	0.9371	0.9853	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:95	YAASITSFL	0.928	0.68	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:15	YAASITSFL	0.9135	0.9598	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:10	YAASITSFL	0.9108	0.9103	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:17	YAASITSFL	0.9077	0.9573	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B42:02	VPYQYPYAA	0.9067	0.5594	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:13	YQYPYAASI	0.9024	0.9449	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:19	YAASITSFL	0.9023	0.745	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:10	YQYPYAASI	0.9019	0.9768	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:67	YQYPYAASI	0.8951	0.9696	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:80	YQYPYAASI	0.8951	0.9696	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:29	YQYPYAASI	0.894	0.9449	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B42:01	VPYQYPYAA	0.8771	0.5493	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:19	YQYPYAASI	0.8769	0.7918	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:46	YQYPYAASI	0.8681	0.9261	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:27	YQYPYAASI	0.8625	0.97	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:06	YQYPYAASI	0.861	0.9804	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:08	YQYPYAASI	0.8594	0.8959	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B07:12	VPYQYPYAA	0.8594	0.5337	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:95	YQYPYAASI	0.8532	0.7773	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:14	YQYPYAASI	0.7975	0.9835	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:30	YAASITSFL	0.7589	0.973	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:17	YQYPYAASI	0.7105	0.9673	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:05	YQYPYAASI	0.6937	0.856	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B14:03	YQYPYAASI	0.5769	0.7393	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B51:07	YQYPYAASI	0.4335	0.9739	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:10	VPYQYPYAA	0.3865	0.7823	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:30	YQYPYAASI	0.3771	0.9668	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:67	PYAASITSF	0.3221	0.8959	10	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:80	PYAASITSF	0.3221	0.8959	10	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:31	YPYAASITSF	0.9993	0.5713	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:21	YPYAASITSF	0.9975	0.7591	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:80	YPYAASITSF	0.9887	0.8884	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:67	YPYAASITSF	0.9887	0.8884	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:12	YPYAASITSF	0.9881	0.7656	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:10	YPYAASITSF	0.988	0.9075	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B54:01	AVPYQYPYAA	0.9472	0.5698	4	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:10	YPYAASITSF	0.9213	0.7623	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B54:01	YAVPYQYPYAA	0.9981	0.5823	3	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:12	YPYAASITSFL	0.997	0.8134	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:10	YPYAASITSFL	0.983	0.8181	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B78:01	YAVPYQYPYAA	0.9756	0.6674	3	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:04	YAASITSF	1	0.9877	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:03	YAASITSF	1	0.9877	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:67	YAASITSF	0.9999	0.9731	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:02	YAASITSF	0.9999	0.97	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:17	YAASITSF	0.9998	0.9725	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:02	YAASITSF	0.9997	0.971	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C16:04	YAASITSF	0.9996	0.9805	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:05	YAASITSF	0.9995	0.9293	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:09	YAASITSF	0.9995	0.8974	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:03	YAASITSF	0.9993	0.9833	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:11	YAASITSF	0.9989	0.8492	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:11	YAASITSF	0.9984	0.843	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:08	YAASITSF	0.9983	0.8442	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C16:01	YAASITSF	0.9977	0.9813	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:43	YAASITSF	0.9977	0.8487	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:06	YAASITSF	0.997	0.9862	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:17	YAASITSF	0.9898	0.6407	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:30	YAASITSF	0.9898	0.6407	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:23	YAASITSF	0.9869	0.7952	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:77	YAASITSF	0.9853	0.7909	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C16:02	YAASITSF	0.985	0.9929	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:10	YAASITSF	0.9838	0.986	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:02	YAASITSF	0.9838	0.986	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:24	YAASITSF	0.9595	0.8448	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B07:13	YAASITSF	0.948	0.8415	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:01	YAASITSF	0.9299	0.9749	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B18:07	YAASITSF	0.8584	0.8021	11	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B07:13	AASITSFL	0.8581	0.8786	12	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:03	YAASITSFL	0.9997	0.9892	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:04	YAASITSFL	0.9997	0.9892	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:67	YAASITSFL	0.9992	0.9785	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:05	YAASITSFL	0.9991	0.9317	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:09	YAASITSFL	0.999	0.9171	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:17	YAASITSFL	0.999	0.9773	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:05	YAASITSFL	0.9989	0.9179	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:02	YAASITSFL	0.9988	0.8828	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:02	YAASITSFL	0.9988	0.9734	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:03	YQYPYAASI	0.9983	0.9887	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:02	YAASITSFL	0.9982	0.9777	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:03	YAASITSFL	0.9975	0.9874	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:04	YAASITSFL	0.9973	0.8234	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C16:04	YAASITSFL	0.9972	0.9851	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:24	YQYPYAASI	0.9963	0.9461	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:06	YAASITSFL	0.996	0.9877	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:17	YQYPYAASI	0.9959	0.9956	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:02	YQYPYAASI	0.9959	0.9956	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:73	YQYPYAASI	0.9957	0.9353	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:02	YQYPYAASI	0.9944	0.9365	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B27:06	YQYPYAASI	0.9927	0.7736	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:27	YQYPYAASI	0.9924	0.9444	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:31	YQYPYAASI	0.9915	0.9336	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:30	YQYPYAASI	0.9908	0.9073	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:06	YQYPYAASI	0.9906	0.5454	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:11	YAASITSFL	0.9904	0.8883	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:03	YQYPYAASI	0.9902	0.5498	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B78:02	VPYQYPYAA	0.9899	0.5995	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:13	YAASITSFL	0.9894	0.8898	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:02	YAASITSFL	0.9893	0.9955	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:17	YAASITSFL	0.9893	0.9955	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:09	YQYPYAASI	0.9891	0.9439	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:04	YQYPYAASI	0.9885	0.9128	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:05	YQYPYAASI	0.9871	0.939	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:01	YAASITSFL	0.9868	0.9843	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C15:02	YQYPYAASI	0.9864	0.9368	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C16:01	YAASITSFL	0.9859	0.9857	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C16:02	YAASITSFL	0.9837	0.9951	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C16:04	YQYPYAASI	0.9832	0.9887	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:53	YQYPYAASI	0.9825	0.895	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A69:01	YAASITSFL	0.9812	0.7647	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:23	YAASITSFL	0.9803	0.8588	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:77	YAASITSFL	0.9794	0.872	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:04	YAASITSFL	0.9787	0.9193	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:35	YQYPYAASI	0.9784	0.9092	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C05:01	YAASITSFL	0.9775	0.9316	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:03	YAASITSFL	0.9775	0.7765	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C17:01	YQYPYAASI	0.9763	0.9721	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:17	YQYPYAASI	0.9757	0.9734	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C04:01	YAASITSFL	0.9755	0.7213	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:06	YAASITSFL	0.974	0.9938	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C18:01	YAASITSFL	0.9734	0.7384	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:14	YAASITSFL	0.9708	0.5277	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A02:06	YAASITSFL	0.97	0.6495	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:77	YPYAASITS	0.9693	0.6562	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:23	YPYAASITS	0.9674	0.6799	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:68	YQYPYAASI	0.967	0.6687	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:01	YQYPYAASI	0.9659	0.9823	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:05	YQYPYAASI	0.9659	0.939	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:30	YAASITSFL	0.964	0.7469	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:17	YAASITSFL	0.964	0.7469	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:54	YQYPYAASI	0.9586	0.8642	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:06	YQYPYAASI	0.958	0.9891	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:09	YAASITSFL	0.9559	0.9458	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:03	YQYPYAASI	0.9498	0.9883	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:04	YQYPYAASI	0.9498	0.9883	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C12:02	YQYPYAASI	0.9433	0.9835	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:09	VPYQYPYAA	0.9424	0.8421	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:09	YPYAASITS	0.9417	0.7775	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:08	YAASITSFL	0.9382	0.8726	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B38:05	YQYPYAASI	0.9368	0.9577	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:67	YQYPYAASI	0.934	0.9823	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:02	YAASITSFL	0.9333	0.9895	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:10	YAASITSFL	0.9333	0.9895	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:22	YAASITSFL	0.9305	0.7597	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:24	YPYAASITS	0.9302	0.6621	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:43	YAASITSFL	0.9296	0.8777	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:30	YAASITSFL	0.9266	0.8573	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:03	YAASITSFL	0.9242	0.9281	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:04	YQYPYAASI	0.921	0.9353	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B39:11	YQYPYAASI	0.9195	0.7631	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:02	YAASITSFL	0.9169	0.9548	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C08:02	YAASITSFL	0.9135	0.9598	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:06	YQYPYAASI	0.9134	0.9925	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:30	VPYQYPYAA	0.9094	0.5204	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:17	VPYQYPYAA	0.9094	0.5204	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A25:01	YAASITSFL	0.9073	0.8657	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:01	YAASITSFL	0.9065	0.6851	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:77	VPYQYPYAA	0.9024	0.6668	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:22	YQYPYAASI	0.9016	0.7503	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:02	YQYPYAASI	0.8951	0.9696	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:23	VPYQYPYAA	0.8947	0.6578	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:17	YQYPYAASI	0.8918	0.9728	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:12	YQYPYAASI	0.8902	0.8905	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:08	YAASITSFL	0.8896	0.9947	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-A32:01	YQYPYAASI	0.8801	0.9526	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C06:08	YQYPYAASI	0.8789	0.9933	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:02	YQYPYAASI	0.8745	0.9781	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B78:02	YAASITSFL	0.8742	0.5308	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:13	YQYPYAASI	0.8572	0.8421	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:02	YQYPYAASI	0.8565	0.9865	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C02:10	YQYPYAASI	0.8565	0.9865	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:01	YQYPYAASI	0.8466	0.764	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C17:01	YAASITSFL	0.8426	0.9409	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:39	YQYPYAASI	0.8366	0.8848	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B07:13	YAASITSFL	0.8228	0.8622	11	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B48:02	YQYPYAASI	0.8212	0.8863	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:24	VPYQYPYAA	0.801	0.6979	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:11	VPYQYPYAA	0.7283	0.7597	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B67:01	YPYAASITS	0.7189	0.5533	9	18
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:02	YQYPYAASI	0.7186	0.9666	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:20	YQYPYAASI	0.6982	0.9049	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:28	YQYPYAASI	0.6907	0.9098	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C01:03	YQYPYAASI	0.6862	0.9584	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:09	YQYPYAASI	0.6847	0.7056	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B67:01	VPYQYPYAA	0.6704	0.7455	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:02	PYAASITSF	0.3221	0.8959	10	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C14:03	PYAASITSF	0.1886	0.9618	10	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C14:02	PYAASITSF	0.1886	0.9618	10	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B07:13	YQYPYAASI	0.1614	0.7661	7	16
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B18:07	VPYQYPYAA	0.1533	0.6695	5	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:77	YPYAASITSF	0.9994	0.6106	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:23	YPYAASITSF	0.9993	0.6382	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:20	YPYAASITSF	0.9992	0.6732	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:24	YPYAASITSF	0.9984	0.6369	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C03:67	YPYAASITSF	0.9982	0.9505	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:11	YPYAASITSF	0.9976	0.7367	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:13	YPYAASITSF	0.9974	0.6857	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C07:02	YPYAASITSF	0.9887	0.8884	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:09	YPYAASITSF	0.9881	0.7656	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:08	YPYAASITSF	0.98	0.6136	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:43	YPYAASITSF	0.9783	0.6149	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B15:11	YPYAASITSF	0.9773	0.613	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B18:04	YPYAASITSF	0.9729	0.7103	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B67:01	YPYAASITSF	0.9496	0.5669	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C14:02	YPYAASITSF	0.9446	0.9476	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C14:03	YPYAASITSF	0.9446	0.9476	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B18:07	YPYAASITSF	0.9145	0.6096	9	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:13	YPYAASITSFL	0.999	0.7366	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:23	YPYAASITSFL	0.999	0.686	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:77	YPYAASITSFL	0.999	0.6845	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B35:09	YPYAASITSFL	0.997	0.8134	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B67:01	YPYAASITSFL	0.9874	0.6279	9	20
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-B78:02	YAVPYQYPYAA	0.9661	0.6843	3	14
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C14:02	QYPYAASITSF	0.9619	0.9794	8	19
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	992	HLA-C14:03	QYPYAASITSF	0.9619	0.9794	8	19

Top

Potential FusionNeoAntigen Information of PLEKHB2-BIRC6 in HLA II

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene

Hchr

Hbp

Tgene

Tchr

Tbp

HLA II

FusionNeoAntigen peptide

Neoantigen start (at BP 13)

Neoantigen end (at BP 13)

Top

Fusion breakpoint peptide structures of PLEKHB2-BIRC6

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
7116	PYQYPYAASITSFL	PLEKHB2	BIRC6	chr2	131897848	chr2	32715108	992

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of PLEKHB2-BIRC6

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	7116	PYQYPYAASITSFL	-8.55667	-8.74867
HLA-B14:02	3BVN	7116	PYQYPYAASITSFL	-3.49457	-4.25557
HLA-B52:01	3W39	7116	PYQYPYAASITSFL	-7.26725	-7.45925
HLA-B52:01	3W39	7116	PYQYPYAASITSFL	-4.01461	-4.77561
HLA-A24:02	5HGA	7116	PYQYPYAASITSFL	-7.65911	-8.42011
HLA-A24:02	5HGA	7116	PYQYPYAASITSFL	-7.10204	-7.29404
HLA-B44:05	3DX8	7116	PYQYPYAASITSFL	-7.5556	-8.3166
HLA-B44:05	3DX8	7116	PYQYPYAASITSFL	-2.76159	-2.95359

Top

Vaccine Design for the FusionNeoAntigens of PLEKHB2-BIRC6

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	10	19	PYAASITSF	CATATGCAGCATCAATAACTAGCTTTC
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	11	19	YAASITSF	ATGCAGCATCAATAACTAGCTTTC
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	11	20	YAASITSFL	ATGCAGCATCAATAACTAGCTTTCTCA
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	12	20	AASITSFL	CAGCATCAATAACTAGCTTTCTCA
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	3	14	YAVPYQYPYAA	ATGCCGTGCCCTACCAGTACCCATATGCAGCAT
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	4	14	AVPYQYPYAA	CCGTGCCCTACCAGTACCCATATGCAGCAT
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	5	14	VPYQYPYAA	TGCCCTACCAGTACCCATATGCAGCAT
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	5	16	VPYQYPYAASI	TGCCCTACCAGTACCCATATGCAGCATCAATAA
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	7	16	YQYPYAASI	ACCAGTACCCATATGCAGCATCAATAA
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	8	16	QYPYAASI	AGTACCCATATGCAGCATCAATAA
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	8	19	QYPYAASITSF	AGTACCCATATGCAGCATCAATAACTAGCTTTC
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	9	18	YPYAASITS	ACCCATATGCAGCATCAATAACTAGCT
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	9	19	YPYAASITSF	ACCCATATGCAGCATCAATAACTAGCTTTC
PLEKHB2-BIRC6	chr2	131897848	chr2	32715108	9	20	YPYAASITSFL	ACCCATATGCAGCATCAATAACTAGCTTTCTCA

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene

Hchr

Hbp

Tchr

Tbp

Start in +/-13AA

End in +/-13AA

FusionNeoAntigen peptide

FusionNEoAntigen RNA sequence

Top

Information of the samples that have these potential fusion neoantigens of PLEKHB2-BIRC6

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
PRAD	PLEKHB2-BIRC6	chr2	131897848	ENST00000234115	chr2	32715108	ENST00000421745	TCGA-CH-5792-01A

Top

Potential target of CAR-T therapy development for PLEKHB2-BIRC6

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

Top

Related Drugs to PLEKHB2-BIRC6

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to PLEKHB2-BIRC6

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)