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Fusion Protein:RFWD2-KIF14

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RFWD2-KIF14
	FusionPDB ID: 73674
	FusionGDB2.0 ID: 73674
		Hgene	Tgene
	Gene symbol	RFWD2	KIF14
	Gene ID	64326	9928
	Gene name	COP1 E3 ubiquitin ligase	kinesin family member 14
	Synonyms	CFAP78\|FAP78\|RFWD2\|RNF200	MCPH20\|MKS12
	Cytomap	1q25.1-q25.2	1q32.1
	Type of gene	protein-coding	protein-coding
	Description	E3 ubiquitin-protein ligase COP1E3 ubiquitin-protein ligase RFWD2RING finger protein 200RING-type E3 ubiquitin transferase RFWD2constitutive photomorphogenesis protein 1 homologconstitutive photomorphogenic protein 1putative ubiquitin ligase COP1ri	kinesin-like protein KIF14epididymis secretory sperm binding protein
	Modification date	20200313	20200315
	UniProtAcc	.	Q15058 Main function of 5'-partner protein: FUNCTION: Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity (By similarity). Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis (PubMed:24784001, PubMed:16648480). During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively (PubMed:16431929). Regulates cell growth through regulation of cell cycle progression and cytokinesis (PubMed:24854087). During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1 (PubMed:24854087). During late neurogenesis, regulates the cerebellar, cerebral cortex and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division (By similarity). Also is required for chromosome congression and alignment during mitotic cell cycle process (PubMed:15843429). Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules (PubMed:23209302). {ECO:0000250\|UniProtKB:L0N7N1, ECO:0000269\|PubMed:15843429, ECO:0000269\|PubMed:16431929, ECO:0000269\|PubMed:16648480, ECO:0000269\|PubMed:23209302, ECO:0000269\|PubMed:24784001, ECO:0000269\|PubMed:24854087}.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000308769, ENST00000367669,	ENST00000367350,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	17 X 15 X 10=2550	3 X 3 X 3=27
	# samples	20	4
	** MAII score	log2(20/2550*10)=-3.6724253419715 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/27*10)=0.567040592723894 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Fusion gene context	PubMed: RFWD2 [Title/Abstract] AND KIF14 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: RFWD2 [Title/Abstract] AND KIF14 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	RFWD2(176118142)-KIF14(200578056), # samples:3
Anticipated loss of major functional domain due to fusion event.	RFWD2-KIF14 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RFWD2-KIF14 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RFWD2-KIF14 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RFWD2-KIF14 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	RFWD2	GO:0010212	response to ionizing radiation	19805145
Tgene	KIF14	GO:0008284	positive regulation of cell proliferation	24784001
Tgene	KIF14	GO:0034446	substrate adhesion-dependent cell spreading	23209302
Tgene	KIF14	GO:0045184	establishment of protein localization	23209302

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:176118142/chr1:200578056)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across RFWD2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across KIF14 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000367669	RFWD2	chr1	176118142	-	ENST00000367350	KIF14	chr1	200578056	-	6726	1346	515	4837	1440
ENST00000308769	RFWD2	chr1	176118142	-	ENST00000367350	KIF14	chr1	200578056	-	6199	819	0	4310	1436
ENST00000367669	RFWD2	chr1	176118141	-	ENST00000367350	KIF14	chr1	200578056	-	6726	1346	515	4837	1440
ENST00000308769	RFWD2	chr1	176118141	-	ENST00000367350	KIF14	chr1	200578056	-	6199	819	0	4310	1436
ENST00000367669	RFWD2	chr1	176132004	-	ENST00000367350	KIF14	chr1	200578056	-	6657	1277	515	4768	1417
ENST00000308769	RFWD2	chr1	176132004	-	ENST00000367350	KIF14	chr1	200578056	-	6130	750	0	4241	1413

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000367669	ENST00000367350	RFWD2	chr1	176118142	-	KIF14	chr1	200578056	-	0.000240048	0.99976
ENST00000308769	ENST00000367350	RFWD2	chr1	176118142	-	KIF14	chr1	200578056	-	0.000142801	0.9998572
ENST00000367669	ENST00000367350	RFWD2	chr1	176118141	-	KIF14	chr1	200578056	-	0.000240048	0.99976
ENST00000308769	ENST00000367350	RFWD2	chr1	176118141	-	KIF14	chr1	200578056	-	0.000142801	0.9998572
ENST00000367669	ENST00000367350	RFWD2	chr1	176132004	-	KIF14	chr1	200578056	-	0.000231514	0.99976844
ENST00000308769	ENST00000367350	RFWD2	chr1	176132004	-	KIF14	chr1	200578056	-	0.00013674	0.99986327

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for RFWD2-KIF14

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
RFWD2	chr1	176118141	KIF14	chr1	200578056	1346	277	MEFLKVARRNKREVSYHIEMSFFEVY
RFWD2	chr1	176118141	KIF14	chr1	200578056	819	273	MEFLKVARRNKREVSYHIEMSFFEVY
RFWD2	chr1	176118142	KIF14	chr1	200578056	1346	277	MEFLKVARRNKREVSYHIEMSFFEVY
RFWD2	chr1	176118142	KIF14	chr1	200578056	819	273	MEFLKVARRNKREVSYHIEMSFFEVY
RFWD2	chr1	176132004	KIF14	chr1	200578056	1277	254	LELLVQKKKQLEAVSYHIEMSFFEVY
RFWD2	chr1	176132004	KIF14	chr1	200578056	750	250	LELLVQKKKQLEAVSYHIEMSFFEVY

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Potential FusionNeoAntigen Information of RFWD2-KIF14 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

RFWD2-KIF14_176118141_200578056.msa
RFWD2-KIF14_176132004_200578056.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:05	RRNKREVSY	0.9993	0.7881	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:04	RRNKREVSY	0.9991	0.6049	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B47:01	REVSYHIEM	0.9982	0.5678	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B13:01	REVSYHIEM	0.9969	0.8818	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B44:03	REVSYHIEM	0.9955	0.946	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:01	REVSYHIEM	0.976	0.8724	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B45:01	REVSYHIEM	0.9256	0.6061	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B39:13	REVSYHIEM	0.8049	0.899	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:03	REVSYHIEM	0.636	0.8932	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B41:01	REVSYHIEM	0.5627	0.6733	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B50:01	REVSYHIEM	0.4733	0.7201	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:03	RRNKREVSY	0.1195	0.668	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:18	RRNKREVSY	0.0909	0.636	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:02	ARRNKREVSY	0.9993	0.5481	6	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:04	KREVSYHIEM	0.9993	0.7024	10	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:04	ARRNKREVSY	0.9992	0.6393	6	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B47:01	REVSYHIEMSF	0.9997	0.6975	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B57:01	REVSYHIEMSF	0.9855	0.9857	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B57:03	REVSYHIEMSF	0.9844	0.9883	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:17	REVSYHIEMSF	0.9682	0.9684	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-A32:13	REVSYHIEMSF	0.9633	0.9441	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:16	REVSYHIEMSF	0.9574	0.9145	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B58:02	REVSYHIEMSF	0.9428	0.9773	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B58:01	REVSYHIEMSF	0.7707	0.9712	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B40:06	REVSYHIEM	0.9989	0.6594	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:14	RRNKREVSY	0.9987	0.7377	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:03	RRNKREVSY	0.9944	0.8157	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:95	RRNKREVSY	0.9714	0.6032	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:27	RRNKREVSY	0.9699	0.9101	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:05	RRNKREVSY	0.9663	0.9346	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:67	RRNKREVSY	0.8916	0.8871	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:80	RRNKREVSY	0.8916	0.8871	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:19	RRNKREVSY	0.8855	0.5447	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:46	RRNKREVSY	0.8447	0.7743	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:10	RRNKREVSY	0.8014	0.9146	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B39:08	REVSYHIEM	0.7782	0.6403	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C12:16	RRNKREVSY	0.0402	0.8643	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B40:03	REVSYHIEMSF	0.9997	0.5247	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:08	RRNKREVSY	0.9993	0.6378	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:10	RRNKREVSY	0.999	0.7708	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B40:04	REVSYHIEM	0.9982	0.7082	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B44:26	REVSYHIEM	0.9955	0.946	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B44:07	REVSYHIEM	0.9955	0.946	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B44:13	REVSYHIEM	0.9955	0.946	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:01	RRNKREVSY	0.989	0.5252	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:11	REVSYHIEM	0.988	0.8893	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:09	RRNKREVSY	0.9855	0.7731	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:06	RRNKREVSY	0.9831	0.6042	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:07	REVSYHIEM	0.9807	0.8318	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:04	REVSYHIEM	0.9804	0.8879	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:08	REVSYHIEM	0.9765	0.707	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:05	REVSYHIEM	0.976	0.8724	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:06	REVSYHIEM	0.9728	0.8776	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B18:03	REVSYHIEM	0.9672	0.8644	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:17	RRNKREVSY	0.9192	0.9265	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:02	RRNKREVSY	0.8916	0.8871	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B39:31	REVSYHIEM	0.8081	0.8982	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:53	REVSYHIEM	0.8057	0.9413	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B39:02	REVSYHIEM	0.7914	0.9045	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:54	REVSYHIEM	0.7565	0.9348	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C07:22	RRNKREVSY	0.7365	0.5817	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B35:28	REVSYHIEM	0.7132	0.9274	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B48:02	REVSYHIEM	0.628	0.9171	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:68	REVSYHIEM	0.622	0.7593	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C06:08	RRNKREVSY	0.5737	0.9278	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B50:05	REVSYHIEM	0.4733	0.7201	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B50:04	REVSYHIEM	0.4733	0.7201	11	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B48:02	RRNKREVSY	0.1606	0.9162	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:54	RRNKREVSY	0.1418	0.821	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:68	RRNKREVSY	0.048	0.5217	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C06:06	RRNKREVSY	0.0457	0.9512	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C06:17	RRNKREVSY	0.0316	0.9621	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-C06:02	RRNKREVSY	0.0316	0.9621	7	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:10	ARRNKREVSY	0.9992	0.8046	6	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:08	KREVSYHIEM	0.9992	0.771	10	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:06	KREVSYHIEM	0.9988	0.7047	10	20
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-A25:01	EVSYHIEMSF	0.9963	0.7883	12	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:68	ARRNKREVSY	0.8792	0.5991	6	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B27:09	RRNKREVSYHI	0.9996	0.7938	7	18
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B15:53	REVSYHIEMSF	0.9984	0.9698	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-A25:01	EVSYHIEMSFF	0.9977	0.783	12	23
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-A32:01	REVSYHIEMSF	0.9862	0.9336	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B57:10	REVSYHIEMSF	0.9855	0.9857	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B48:02	REVSYHIEMSF	0.9805	0.9517	11	22
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	HLA-B58:06	REVSYHIEMSF	0.9738	0.9643	11	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:01	KQLEAVSY	0.9996	0.8166	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:25	KQLEAVSY	0.9812	0.8779	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B47:01	KQLEAVSY	0.9467	0.5258	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:03	KQLEAVSY	0.8267	0.7122	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:08	EAVSYHIEM	0.9983	0.8422	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:01	EAVSYHIEM	0.9964	0.8979	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:03	EAVSYHIEM	0.9921	0.9147	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:05	EAVSYHIEM	0.9823	0.5812	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:04	EAVSYHIEM	0.9404	0.9141	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:02	EAVSYHIEM	0.9404	0.9141	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B47:01	KQLEAVSYH	0.5854	0.5441	8	17
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:03	KKQLEAVSY	0.2242	0.5961	7	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:18	KKQLEAVSY	0.2039	0.5064	7	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:03	KQLEAVSYH	0.2003	0.6826	8	17
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B57:01	AVSYHIEMSF	0.9959	0.991	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:17	AVSYHIEMSF	0.9921	0.9748	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:16	AVSYHIEMSF	0.9912	0.9255	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B57:03	AVSYHIEMSF	0.9764	0.9947	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-A32:13	AVSYHIEMSF	0.8817	0.9655	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B13:01	KQLEAVSYHI	0.8086	0.8794	8	18
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:03	KKKQLEAVSY	0.6921	0.5547	6	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B52:01	KQLEAVSYHI	0.1523	0.9155	8	18
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:07	KQLEAVSY	0.9952	0.532	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:04	KQLEAVSY	0.9498	0.8313	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:05	KQLEAVSY	0.8667	0.8764	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:08	EAVSYHIEM	0.9993	0.8554	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:19	EAVSYHIEM	0.9993	0.9879	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:07	EAVSYHIEM	0.9986	0.9837	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:21	EAVSYHIEM	0.9965	0.9207	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:12	EAVSYHIEM	0.9404	0.9141	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C12:12	EAVSYHIEM	0.9194	0.926	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B51:07	EAVSYHIEM	0.8963	0.7771	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C08:03	EAVSYHIEM	0.8898	0.9407	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:14	EAVSYHIEM	0.8692	0.9839	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C06:03	EAVSYHIEM	0.8572	0.9952	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C12:04	EAVSYHIEM	0.8428	0.9955	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:04	KQLEAVSYH	0.8005	0.8215	8	17
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:27	KQLEAVSY	0.9996	0.8352	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:125	KQLEAVSY	0.9996	0.8166	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:33	KQLEAVSY	0.9996	0.8166	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:34	KQLEAVSY	0.9996	0.8166	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:135	KQLEAVSY	0.9996	0.8213	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:50	KQLEAVSY	0.9995	0.8169	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:12	KQLEAVSY	0.998	0.7135	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:53	KQLEAVSY	0.9976	0.7576	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:54	KQLEAVSY	0.9949	0.7393	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:35	KQLEAVSY	0.9917	0.7819	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:39	KQLEAVSY	0.9828	0.7878	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:20	KQLEAVSY	0.8753	0.9153	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:28	KQLEAVSY	0.8495	0.9217	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B18:04	KQLEAVSY	0.8167	0.9011	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B48:02	KQLEAVSY	0.5949	0.9064	8	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:03	EAVSYHIEM	0.9996	0.987	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:04	EAVSYHIEM	0.9996	0.987	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-A25:01	EAVSYHIEM	0.9992	0.8029	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:05	EAVSYHIEM	0.999	0.9088	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:17	EAVSYHIEM	0.999	0.9676	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:02	EAVSYHIEM	0.9988	0.9712	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:67	EAVSYHIEM	0.9981	0.9792	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:11	EAVSYHIEM	0.9966	0.9164	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:77	EAVSYHIEM	0.9964	0.8979	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:23	EAVSYHIEM	0.9961	0.8734	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:20	EAVSYHIEM	0.9953	0.9193	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:13	EAVSYHIEM	0.9905	0.9181	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C16:04	EAVSYHIEM	0.988	0.9851	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-A69:01	EAVSYHIEM	0.9864	0.6783	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:17	EAVSYHIEM	0.9846	0.7615	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:30	EAVSYHIEM	0.9846	0.7615	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C12:02	EAVSYHIEM	0.9814	0.9752	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:24	EAVSYHIEM	0.9771	0.873	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C03:06	EAVSYHIEM	0.9653	0.9896	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:50	KQLEAVSYH	0.9623	0.8351	8	17
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:09	EAVSYHIEM	0.9404	0.9141	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C12:03	EAVSYHIEM	0.935	0.9823	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:11	EAVSYHIEM	0.9107	0.8197	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:08	EAVSYHIEM	0.9081	0.8471	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:43	EAVSYHIEM	0.8978	0.8389	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C08:01	EAVSYHIEM	0.8898	0.9407	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B78:02	EAVSYHIEM	0.8844	0.5901	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:35	KQLEAVSYH	0.7985	0.7838	8	17
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-C16:01	EAVSYHIEM	0.7858	0.9775	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:54	KQLEAVSYH	0.6936	0.7157	8	17
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B18:04	EAVSYHIEM	0.6721	0.9287	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B18:07	EAVSYHIEM	0.3742	0.8929	11	20
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B35:28	KKQLEAVSY	0.3197	0.8267	7	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B48:02	KKQLEAVSY	0.2131	0.7977	7	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:53	KKQLEAVSY	0.1974	0.7191	7	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:54	KKQLEAVSY	0.0712	0.688	7	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B57:10	AVSYHIEMSF	0.9959	0.991	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B58:06	AVSYHIEMSF	0.989	0.9709	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-A32:01	AVSYHIEMSF	0.9607	0.9781	12	22
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:54	KKKQLEAVSY	0.9245	0.6473	6	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B15:53	KKKQLEAVSY	0.9045	0.6911	6	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	HLA-B48:02	KKKQLEAVSY	0.6309	0.7484	6	16

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Potential FusionNeoAntigen Information of RFWD2-KIF14 in HLA II

RFWD2-KIF14_176118141_200578056.msa
RFWD2-KIF14_176132004_200578056.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-0303	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1102	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1102	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1103	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1111	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1114	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1116	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1116	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1120	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1121	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1121	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1136	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1136	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1141	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1148	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1148	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1155	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1155	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1159	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1159	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1163	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1165	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1165	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1168	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1170	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1170	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1176	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1185	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1186	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1186	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1301	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1301	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1302	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1308	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1308	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1315	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1315	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1316	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1316	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1317	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1317	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1319	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1319	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1320	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1320	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1322	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1322	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1323	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1324	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1327	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1327	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1329	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1334	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1335	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1335	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1336	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1339	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1341	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1343	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1351	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1351	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1352	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1352	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1353	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1353	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1357	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1357	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1359	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1359	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1361	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1361	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1363	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1364	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1364	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1368	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1368	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1369	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1369	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1370	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1370	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1371	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1371	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1372	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1372	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1373	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1374	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1376	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1376	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1378	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1378	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1379	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1379	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1380	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1380	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1383	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1383	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1384	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1384	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1387	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1387	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1391	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1391	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1392	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1392	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1396	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1396	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1397	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1398	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1398	EFLKVARRNKREVSY	1	16
RFWD2-KIF14	chr1	176118141	chr1	200578056	819	DRB1-1399	MEFLKVARRNKREVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1102	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1103	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1116	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1121	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1136	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1141	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1148	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1155	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1159	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1163	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1165	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1170	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1176	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1185	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1301	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1304	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1304	ELLVQKKKQLEAVSY	1	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1308	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1315	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1317	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1319	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1320	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1322	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1324	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1327	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1332	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1332	ELLVQKKKQLEAVSY	1	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1335	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1338	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1338	ELLVQKKKQLEAVSY	1	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1343	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1348	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1348	ELLVQKKKQLEAVSY	1	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1351	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1352	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1353	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1354	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1357	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1359	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1361	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1364	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1365	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1365	ELLVQKKKQLEAVSY	1	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1368	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1369	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1370	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1371	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1372	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1375	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1375	ELLVQKKKQLEAVSY	1	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1376	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1378	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1379	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1380	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1383	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1384	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1387	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1391	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1392	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1393	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1393	ELLVQKKKQLEAVSY	1	16
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1398	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1416	LELLVQKKKQLEAVS	0	15
RFWD2-KIF14	chr1	176132004	chr1	200578056	750	DRB1-1478	LELLVQKKKQLEAVS	0	15

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Fusion breakpoint peptide structures of RFWD2-KIF14

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
565	ARRNKREVSYHIEM	RFWD2	KIF14	chr1	176118141	chr1	200578056	819

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
4345	KKKQLEAVSYHIEM	RFWD2	KIF14	chr1	176132004	chr1	200578056	750

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of RFWD2-KIF14

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	565	ARRNKREVSYHIEM	-6.18902	-6.30242
HLA-B14:02	3BVN	565	ARRNKREVSYHIEM	-5.51674	-6.55204
HLA-B52:01	3W39	565	ARRNKREVSYHIEM	-6.26372	-6.37712
HLA-B52:01	3W39	565	ARRNKREVSYHIEM	-2.88933	-3.92463
HLA-A11:01	4UQ2	565	ARRNKREVSYHIEM	-8.95966	-9.99496
HLA-A24:02	5HGA	565	ARRNKREVSYHIEM	-7.97421	-8.08761
HLA-A24:02	5HGA	565	ARRNKREVSYHIEM	-4.46014	-5.49544
HLA-B44:05	3DX8	565	ARRNKREVSYHIEM	-4.21738	-4.33078
HLA-B44:05	3DX8	565	ARRNKREVSYHIEM	-3.79801	-4.83331
HLA-A02:01	6TDR	565	ARRNKREVSYHIEM	-6.07498	-7.11028
HLA-B14:02	3BVN	4345	KKKQLEAVSYHIEM	-7.15543	-7.26883
HLA-B14:02	3BVN	4345	KKKQLEAVSYHIEM	-4.77435	-5.80965
HLA-B52:01	3W39	4345	KKKQLEAVSYHIEM	-6.80875	-6.92215
HLA-B52:01	3W39	4345	KKKQLEAVSYHIEM	-4.20386	-5.23916
HLA-A11:01	4UQ2	4345	KKKQLEAVSYHIEM	-7.5194	-8.5547
HLA-A11:01	4UQ2	4345	KKKQLEAVSYHIEM	-6.9601	-7.0735
HLA-A24:02	5HGA	4345	KKKQLEAVSYHIEM	-7.52403	-7.63743
HLA-A24:02	5HGA	4345	KKKQLEAVSYHIEM	-5.82433	-6.85963
HLA-B27:05	6PYJ	4345	KKKQLEAVSYHIEM	-3.28285	-4.31815
HLA-B44:05	3DX8	4345	KKKQLEAVSYHIEM	-5.91172	-6.94702
HLA-B44:05	3DX8	4345	KKKQLEAVSYHIEM	-4.24346	-4.35686

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Vaccine Design for the FusionNeoAntigens of RFWD2-KIF14

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
RFWD2-KIF14	chr1	176118141	chr1	200578056	10	20	KREVSYHIEM	AAGAGAGAGGTCAGCTATCACATTGAAATG
RFWD2-KIF14	chr1	176118141	chr1	200578056	11	20	REVSYHIEM	AGAGAGGTCAGCTATCACATTGAAATG
RFWD2-KIF14	chr1	176118141	chr1	200578056	11	22	REVSYHIEMSF	AGAGAGGTCAGCTATCACATTGAAATGAGCTTC
RFWD2-KIF14	chr1	176118141	chr1	200578056	12	22	EVSYHIEMSF	GAGGTCAGCTATCACATTGAAATGAGCTTC
RFWD2-KIF14	chr1	176118141	chr1	200578056	12	23	EVSYHIEMSFF	GAGGTCAGCTATCACATTGAAATGAGCTTCTTT
RFWD2-KIF14	chr1	176118141	chr1	200578056	6	16	ARRNKREVSY	GCAAGAAGAAATAAGAGAGAGGTCAGCTAT
RFWD2-KIF14	chr1	176118141	chr1	200578056	7	16	RRNKREVSY	AGAAGAAATAAGAGAGAGGTCAGCTAT
RFWD2-KIF14	chr1	176118141	chr1	200578056	7	18	RRNKREVSYHI	AGAAGAAATAAGAGAGAGGTCAGCTATCACATT
RFWD2-KIF14	chr1	176132004	chr1	200578056	11	20	EAVSYHIEM	GAAGCAGTCAGCTATCACATTGAAATG
RFWD2-KIF14	chr1	176132004	chr1	200578056	12	22	AVSYHIEMSF	GCAGTCAGCTATCACATTGAAATGAGCTTC
RFWD2-KIF14	chr1	176132004	chr1	200578056	6	16	KKKQLEAVSY	AAGAAGAAACAACTGGAAGCAGTCAGCTAT
RFWD2-KIF14	chr1	176132004	chr1	200578056	7	16	KKQLEAVSY	AAGAAACAACTGGAAGCAGTCAGCTAT
RFWD2-KIF14	chr1	176132004	chr1	200578056	8	16	KQLEAVSY	AAACAACTGGAAGCAGTCAGCTAT
RFWD2-KIF14	chr1	176132004	chr1	200578056	8	17	KQLEAVSYH	AAACAACTGGAAGCAGTCAGCTATCAC
RFWD2-KIF14	chr1	176132004	chr1	200578056	8	18	KQLEAVSYHI	AAACAACTGGAAGCAGTCAGCTATCACATT

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
RFWD2-KIF14	chr1	176118141	chr1	200578056	0	15	MEFLKVARRNKREVS	ATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGGTCAGC
RFWD2-KIF14	chr1	176118141	chr1	200578056	1	16	EFLKVARRNKREVSY	GAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGGTCAGCTAT
RFWD2-KIF14	chr1	176132004	chr1	200578056	0	15	LELLVQKKKQLEAVS	TTGGAGTTACTAGTGCAGAAGAAGAAACAACTGGAAGCAGTCAGC
RFWD2-KIF14	chr1	176132004	chr1	200578056	1	16	ELLVQKKKQLEAVSY	GAGTTACTAGTGCAGAAGAAGAAACAACTGGAAGCAGTCAGCTAT

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Information of the samples that have these potential fusion neoantigens of RFWD2-KIF14

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
BRCA	RFWD2-KIF14	chr1	176118141	ENST00000308769	chr1	200578056	ENST00000367350	TCGA-BH-A203
BRCA	RFWD2-KIF14	chr1	176132004	ENST00000308769	chr1	200578056	ENST00000367350	TCGA-BH-A203

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Potential target of CAR-T therapy development for RFWD2-KIF14

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to RFWD2-KIF14

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to RFWD2-KIF14

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)