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Fusion Protein:RNF145-PDE4D

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RNF145-PDE4D
	FusionPDB ID: 74828
	FusionGDB2.0 ID: 74828
		Hgene	Tgene
	Gene symbol	RNF145	PDE4D
	Gene ID	153830	5144
	Gene name	ring finger protein 145	phosphodiesterase 4D
	Synonyms	-	ACRDYS2\|DPDE3\|HSPDE4D\|PDE43\|PDE4DN2\|STRK1
	Cytomap	5q33.3	5q11.2-q12.1
	Type of gene	protein-coding	protein-coding
	Description	RING finger protein 145	cAMP-specific 3',5'-cyclic phosphodiesterase 4DcAMP-specific phosphodiesterase PDE4D6phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)testicular tissue protein Li 136
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000274542, ENST00000424310, ENST00000518802, ENST00000519865, ENST00000520638, ENST00000521606, ENST00000518284,	ENST00000317118, ENST00000358923, ENST00000405755, ENST00000503258, ENST00000503947, ENST00000340635, ENST00000360047, ENST00000502484, ENST00000502575, ENST00000507116, ENST00000546160,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 5 X 4=120	20 X 19 X 7=2660
	# samples	6	25
	** MAII score	log2(6/120*10)=-1 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(25/2660*10)=-3.41142624572647 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: RNF145 [Title/Abstract] AND PDE4D [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: RNF145 [Title/Abstract] AND PDE4D [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	RNF145(158596687)-PDE4D(58511794), # samples:3
Anticipated loss of major functional domain due to fusion event.	RNF145-PDE4D seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RNF145-PDE4D seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RNF145-PDE4D seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RNF145-PDE4D seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. RNF145-PDE4D seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. RNF145-PDE4D seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:158596687/chr5:58511794)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across RNF145 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across PDE4D (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000274542	RNF145	chr5	158596687	-	ENST00000340635	PDE4D	chr5	58511794	-	8650	1049	27	3023	998
ENST00000274542	RNF145	chr5	158596687	-	ENST00000360047	PDE4D	chr5	58511794	-	4000	1049	27	3023	998
ENST00000274542	RNF145	chr5	158596687	-	ENST00000507116	PDE4D	chr5	58511794	-	4000	1049	27	3023	998
ENST00000274542	RNF145	chr5	158596687	-	ENST00000502484	PDE4D	chr5	58511794	-	3031	1049	27	3023	998
ENST00000274542	RNF145	chr5	158596687	-	ENST00000546160	PDE4D	chr5	58511794	-	3024	1049	27	3023	998
ENST00000274542	RNF145	chr5	158596687	-	ENST00000502575	PDE4D	chr5	58511794	-	1699	1049	27	1445	472
ENST00000424310	RNF145	chr5	158596687	-	ENST00000340635	PDE4D	chr5	58511794	-	8899	1298	264	3272	1002
ENST00000424310	RNF145	chr5	158596687	-	ENST00000360047	PDE4D	chr5	58511794	-	4249	1298	264	3272	1002
ENST00000424310	RNF145	chr5	158596687	-	ENST00000507116	PDE4D	chr5	58511794	-	4249	1298	264	3272	1002
ENST00000424310	RNF145	chr5	158596687	-	ENST00000502484	PDE4D	chr5	58511794	-	3280	1298	264	3272	1002
ENST00000424310	RNF145	chr5	158596687	-	ENST00000546160	PDE4D	chr5	58511794	-	3273	1298	264	3272	1002
ENST00000424310	RNF145	chr5	158596687	-	ENST00000502575	PDE4D	chr5	58511794	-	1948	1298	264	1694	476
ENST00000521606	RNF145	chr5	158596687	-	ENST00000340635	PDE4D	chr5	58511794	-	8826	1225	236	3199	987
ENST00000521606	RNF145	chr5	158596687	-	ENST00000360047	PDE4D	chr5	58511794	-	4176	1225	236	3199	987
ENST00000521606	RNF145	chr5	158596687	-	ENST00000507116	PDE4D	chr5	58511794	-	4176	1225	236	3199	987
ENST00000521606	RNF145	chr5	158596687	-	ENST00000502484	PDE4D	chr5	58511794	-	3207	1225	236	3199	987
ENST00000521606	RNF145	chr5	158596687	-	ENST00000546160	PDE4D	chr5	58511794	-	3200	1225	236	3199	988
ENST00000521606	RNF145	chr5	158596687	-	ENST00000502575	PDE4D	chr5	58511794	-	1875	1225	236	1621	461
ENST00000518802	RNF145	chr5	158596687	-	ENST00000340635	PDE4D	chr5	58511794	-	8779	1178	33	3152	1039
ENST00000518802	RNF145	chr5	158596687	-	ENST00000360047	PDE4D	chr5	58511794	-	4129	1178	33	3152	1039
ENST00000518802	RNF145	chr5	158596687	-	ENST00000507116	PDE4D	chr5	58511794	-	4129	1178	33	3152	1039
ENST00000518802	RNF145	chr5	158596687	-	ENST00000502484	PDE4D	chr5	58511794	-	3160	1178	33	3152	1039
ENST00000518802	RNF145	chr5	158596687	-	ENST00000546160	PDE4D	chr5	58511794	-	3153	1178	33	3152	1039
ENST00000518802	RNF145	chr5	158596687	-	ENST00000502575	PDE4D	chr5	58511794	-	1828	1178	33	1574	513
ENST00000520638	RNF145	chr5	158596687	-	ENST00000340635	PDE4D	chr5	58511794	-	8738	1137	136	3111	991
ENST00000520638	RNF145	chr5	158596687	-	ENST00000360047	PDE4D	chr5	58511794	-	4088	1137	136	3111	991
ENST00000520638	RNF145	chr5	158596687	-	ENST00000507116	PDE4D	chr5	58511794	-	4088	1137	136	3111	991
ENST00000520638	RNF145	chr5	158596687	-	ENST00000502484	PDE4D	chr5	58511794	-	3119	1137	136	3111	991
ENST00000520638	RNF145	chr5	158596687	-	ENST00000546160	PDE4D	chr5	58511794	-	3112	1137	136	3111	992
ENST00000520638	RNF145	chr5	158596687	-	ENST00000502575	PDE4D	chr5	58511794	-	1787	1137	136	1533	465
ENST00000274542	RNF145	chr5	158595881	-	ENST00000340635	PDE4D	chr5	58511794	-	8833	1232	27	3206	1059
ENST00000274542	RNF145	chr5	158595881	-	ENST00000360047	PDE4D	chr5	58511794	-	4183	1232	27	3206	1059
ENST00000274542	RNF145	chr5	158595881	-	ENST00000507116	PDE4D	chr5	58511794	-	4183	1232	27	3206	1059
ENST00000274542	RNF145	chr5	158595881	-	ENST00000502484	PDE4D	chr5	58511794	-	3214	1232	27	3206	1059
ENST00000274542	RNF145	chr5	158595881	-	ENST00000546160	PDE4D	chr5	58511794	-	3207	1232	27	3206	1059
ENST00000274542	RNF145	chr5	158595881	-	ENST00000502575	PDE4D	chr5	58511794	-	1882	1232	27	1628	533
ENST00000424310	RNF145	chr5	158595881	-	ENST00000340635	PDE4D	chr5	58511794	-	9082	1481	264	3455	1063
ENST00000424310	RNF145	chr5	158595881	-	ENST00000360047	PDE4D	chr5	58511794	-	4432	1481	264	3455	1063
ENST00000424310	RNF145	chr5	158595881	-	ENST00000507116	PDE4D	chr5	58511794	-	4432	1481	264	3455	1063
ENST00000424310	RNF145	chr5	158595881	-	ENST00000502484	PDE4D	chr5	58511794	-	3463	1481	264	3455	1063
ENST00000424310	RNF145	chr5	158595881	-	ENST00000546160	PDE4D	chr5	58511794	-	3456	1481	264	3455	1063
ENST00000424310	RNF145	chr5	158595881	-	ENST00000502575	PDE4D	chr5	58511794	-	2131	1481	264	1877	537
ENST00000521606	RNF145	chr5	158595881	-	ENST00000340635	PDE4D	chr5	58511794	-	9009	1408	236	3382	1048
ENST00000521606	RNF145	chr5	158595881	-	ENST00000360047	PDE4D	chr5	58511794	-	4359	1408	236	3382	1048
ENST00000521606	RNF145	chr5	158595881	-	ENST00000507116	PDE4D	chr5	58511794	-	4359	1408	236	3382	1048
ENST00000521606	RNF145	chr5	158595881	-	ENST00000502484	PDE4D	chr5	58511794	-	3390	1408	236	3382	1048
ENST00000521606	RNF145	chr5	158595881	-	ENST00000546160	PDE4D	chr5	58511794	-	3383	1408	236	3382	1049
ENST00000521606	RNF145	chr5	158595881	-	ENST00000502575	PDE4D	chr5	58511794	-	2058	1408	236	1804	522
ENST00000518802	RNF145	chr5	158595881	-	ENST00000340635	PDE4D	chr5	58511794	-	8962	1361	33	3335	1100
ENST00000518802	RNF145	chr5	158595881	-	ENST00000360047	PDE4D	chr5	58511794	-	4312	1361	33	3335	1100
ENST00000518802	RNF145	chr5	158595881	-	ENST00000507116	PDE4D	chr5	58511794	-	4312	1361	33	3335	1100
ENST00000518802	RNF145	chr5	158595881	-	ENST00000502484	PDE4D	chr5	58511794	-	3343	1361	33	3335	1100
ENST00000518802	RNF145	chr5	158595881	-	ENST00000546160	PDE4D	chr5	58511794	-	3336	1361	33	3335	1100
ENST00000518802	RNF145	chr5	158595881	-	ENST00000502575	PDE4D	chr5	58511794	-	2011	1361	33	1757	574
ENST00000520638	RNF145	chr5	158595881	-	ENST00000340635	PDE4D	chr5	58511794	-	8921	1320	136	3294	1052
ENST00000520638	RNF145	chr5	158595881	-	ENST00000360047	PDE4D	chr5	58511794	-	4271	1320	136	3294	1052
ENST00000520638	RNF145	chr5	158595881	-	ENST00000507116	PDE4D	chr5	58511794	-	4271	1320	136	3294	1052
ENST00000520638	RNF145	chr5	158595881	-	ENST00000502484	PDE4D	chr5	58511794	-	3302	1320	136	3294	1052
ENST00000520638	RNF145	chr5	158595881	-	ENST00000546160	PDE4D	chr5	58511794	-	3295	1320	136	3294	1053
ENST00000520638	RNF145	chr5	158595881	-	ENST00000502575	PDE4D	chr5	58511794	-	1970	1320	136	1716	526

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000274542	ENST00000340635	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	8.77E-05	0.99991226
ENST00000274542	ENST00000360047	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000281664	0.99971837
ENST00000274542	ENST00000507116	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000281664	0.99971837
ENST00000274542	ENST00000502484	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000487972	0.9995121
ENST00000274542	ENST00000546160	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000488623	0.99951136
ENST00000274542	ENST00000502575	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.001130106	0.99886984
ENST00000424310	ENST00000340635	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	3.71E-05	0.9999629
ENST00000424310	ENST00000360047	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000167296	0.99983263
ENST00000424310	ENST00000507116	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000167296	0.99983263
ENST00000424310	ENST00000502484	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.00029908	0.9997009
ENST00000424310	ENST00000546160	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000299851	0.9997002
ENST00000424310	ENST00000502575	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000818729	0.9991812
ENST00000521606	ENST00000340635	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	8.36E-05	0.9999163
ENST00000521606	ENST00000360047	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000288584	0.99971133
ENST00000521606	ENST00000507116	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000288584	0.99971133
ENST00000521606	ENST00000502484	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000464338	0.9995357
ENST00000521606	ENST00000546160	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000465538	0.9995345
ENST00000521606	ENST00000502575	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.001043636	0.9989563
ENST00000518802	ENST00000340635	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000144296	0.99985564
ENST00000518802	ENST00000360047	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000397469	0.9996025
ENST00000518802	ENST00000507116	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000397469	0.9996025
ENST00000518802	ENST00000502484	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000643635	0.9993563
ENST00000518802	ENST00000546160	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000646446	0.9993536
ENST00000518802	ENST00000502575	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.001902064	0.99809796
ENST00000520638	ENST00000340635	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	7.76E-05	0.9999224
ENST00000520638	ENST00000360047	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000249048	0.9997509
ENST00000520638	ENST00000507116	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000249048	0.9997509
ENST00000520638	ENST00000502484	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000394921	0.9996051
ENST00000520638	ENST00000546160	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.000394458	0.9996056
ENST00000520638	ENST00000502575	RNF145	chr5	158596687	-	PDE4D	chr5	58511794	-	0.001179678	0.99882025
ENST00000274542	ENST00000340635	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	8.80E-05	0.9999119
ENST00000274542	ENST00000360047	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000340248	0.9996598
ENST00000274542	ENST00000507116	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000340248	0.9996598
ENST00000274542	ENST00000502484	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000596996	0.999403
ENST00000274542	ENST00000546160	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000598876	0.9994011
ENST00000274542	ENST00000502575	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.001356951	0.9986431
ENST00000424310	ENST00000340635	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000136836	0.99986315
ENST00000424310	ENST00000360047	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000608411	0.99939156
ENST00000424310	ENST00000507116	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000608411	0.99939156
ENST00000424310	ENST00000502484	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.001048852	0.9989511
ENST00000424310	ENST00000546160	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.001052431	0.99894756
ENST00000424310	ENST00000502575	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.00095244	0.9990476
ENST00000521606	ENST00000340635	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000114594	0.99988544
ENST00000521606	ENST00000360047	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000430185	0.99956983
ENST00000521606	ENST00000507116	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000430185	0.99956983
ENST00000521606	ENST00000502484	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000730018	0.99927
ENST00000521606	ENST00000546160	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000732429	0.9992675
ENST00000521606	ENST00000502575	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.001294067	0.998706
ENST00000518802	ENST00000340635	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000102802	0.99989724
ENST00000518802	ENST00000360047	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000363102	0.9996369
ENST00000518802	ENST00000507116	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000363102	0.9996369
ENST00000518802	ENST00000502484	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000595215	0.9994048
ENST00000518802	ENST00000546160	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000598159	0.9994018
ENST00000518802	ENST00000502575	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.002051306	0.9979487
ENST00000520638	ENST00000340635	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	9.89E-05	0.9999012
ENST00000520638	ENST00000360047	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000339613	0.9996604
ENST00000520638	ENST00000507116	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000339613	0.9996604
ENST00000520638	ENST00000502484	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000557605	0.9994424
ENST00000520638	ENST00000546160	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.000558897	0.9994411
ENST00000520638	ENST00000502575	RNF145	chr5	158595881	-	PDE4D	chr5	58511794	-	0.00125445	0.99874556

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for RNF145-PDE4D

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
RNF145	chr5	158595881	PDE4D	chr5	58511794	1232	402	DPIVLALGASRDNFDVDNGTSAGRSP
RNF145	chr5	158595881	PDE4D	chr5	58511794	1320	395	DPIVLALGASRDNFDVDNGTSAGRSP
RNF145	chr5	158595881	PDE4D	chr5	58511794	1361	443	DPIVLALGASRDNFDVDNGTSAGRSP
RNF145	chr5	158595881	PDE4D	chr5	58511794	1408	391	DPIVLALGASRDNFDVDNGTSAGRSP
RNF145	chr5	158595881	PDE4D	chr5	58511794	1481	406	DPIVLALGASRDNFDVDNGTSAGRSP
RNF145	chr5	158596687	PDE4D	chr5	58511794	1049	341	GYRAFMNDPAMNRFDVDNGTSAGRSP
RNF145	chr5	158596687	PDE4D	chr5	58511794	1137	334	GYRAFMNDPAMNRFDVDNGTSAGRSP
RNF145	chr5	158596687	PDE4D	chr5	58511794	1178	382	GYRAFMNDPAMNRFDVDNGTSAGRSP
RNF145	chr5	158596687	PDE4D	chr5	58511794	1225	330	GYRAFMNDPAMNRFDVDNGTSAGRSP
RNF145	chr5	158596687	PDE4D	chr5	58511794	1298	345	GYRAFMNDPAMNRFDVDNGTSAGRSP

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Potential FusionNeoAntigen Information of RNF145-PDE4D in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

RNF145-PDE4D_158596687_58511794.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:01	FMNDPAMNRF	0.9996	0.9601	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:02	FMNDPAMNRF	0.9958	0.9749	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:25	FMNDPAMNRF	0.9943	0.9654	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B39:06	NRFDVDNGTSA	0.9991	0.8623	11	22
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:10	MNDPAMNRF	0.9996	0.7027	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:07	MNDPAMNRF	0.9995	0.711	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C05:09	MNDPAMNRF	0.9994	0.9809	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C08:15	MNDPAMNRF	0.9987	0.979	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:06	MNDPAMNRF	0.9641	0.9105	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B73:01	NRFDVDNGT	0.9101	0.5391	11	20
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C08:03	MNDPAMNRF	0.8792	0.9923	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:14	MNDPAMNRF	0.7677	0.8103	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C05:09	FMNDPAMNRF	0.9999	0.982	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:10	FMNDPAMNRF	0.9997	0.6799	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C08:15	FMNDPAMNRF	0.9996	0.9828	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:07	FMNDPAMNRF	0.9995	0.7057	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:21	FMNDPAMNRF	0.9952	0.9703	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-A02:07	FMNDPAMNRF	0.9883	0.721	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B73:01	NRFDVDNGTS	0.9487	0.606	11	21
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B73:01	NRFDVDNGTSA	0.9998	0.7383	11	22
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:10	AFMNDPAMNRF	0.9997	0.7381	3	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:07	AFMNDPAMNRF	0.9996	0.7653	3	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:14	AFMNDPAMNRF	0.9986	0.7545	3	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:01	MNDPAMNRF	0.9995	0.711	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:03	MNDPAMNRF	0.9995	0.773	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C05:01	MNDPAMNRF	0.9994	0.9809	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C18:01	MNDPAMNRF	0.9993	0.7434	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C08:02	MNDPAMNRF	0.9987	0.979	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:04	MNDPAMNRF	0.9483	0.9056	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C08:01	MNDPAMNRF	0.8792	0.9923	5	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:03	FMNDPAMNRF	0.9999	0.7495	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C05:01	FMNDPAMNRF	0.9999	0.982	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C18:01	FMNDPAMNRF	0.9997	0.7118	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:24	FMNDPAMNRF	0.9997	0.9265	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:34	FMNDPAMNRF	0.9996	0.9601	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:27	FMNDPAMNRF	0.9996	0.9493	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:33	FMNDPAMNRF	0.9996	0.9601	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:125	FMNDPAMNRF	0.9996	0.9601	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C08:02	FMNDPAMNRF	0.9996	0.9828	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:12	FMNDPAMNRF	0.9995	0.9164	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:135	FMNDPAMNRF	0.9995	0.953	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:01	FMNDPAMNRF	0.9995	0.7057	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:39	FMNDPAMNRF	0.9946	0.924	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-B15:13	FMNDPAMNRF	0.9895	0.7192	4	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C04:01	AFMNDPAMNRF	0.9996	0.7653	3	14
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	HLA-C18:01	AFMNDPAMNRF	0.9995	0.7646	3	14

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Potential FusionNeoAntigen Information of RNF145-PDE4D in HLA II

RNF145-PDE4D_158595881_58511794.msa
RNF145-PDE4D_158596687_58511794.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0434	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0434	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0464	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0464	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0466	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0466	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0472	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-0472	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB1-1002	DPIVLALGASRDNFD	0	15
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0101	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0101	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0101	ASRDNFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0104	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0104	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0104	ASRDNFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0105	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0105	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0105	ASRDNFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0108	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0108	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0108	ASRDNFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0109	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0109	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0111	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0111	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0111	ASRDNFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0112	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0112	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0112	ASRDNFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0113	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0113	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0113	ASRDNFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0114	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0114	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0209	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0212	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0213	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0213	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0216	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0219	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0221	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0222	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0303	RDNFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158595881	chr5	58511794	1232	DRB3-0303	SRDNFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0305	YRAFMNDPAMNRFDV	1	16
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0305	GYRAFMNDPAMNRFD	0	15
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0305	RAFMNDPAMNRFDVD	2	17
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0338	YRAFMNDPAMNRFDV	1	16
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0338	GYRAFMNDPAMNRFD	0	15
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0340	YRAFMNDPAMNRFDV	1	16
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0340	GYRAFMNDPAMNRFD	0	15
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0340	RAFMNDPAMNRFDVD	2	17
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0434	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0466	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0472	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB1-0472	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0101	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0101	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0104	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0104	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0105	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0105	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0105	PAMNRFDVDNGTSAG	8	23
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0105	GYRAFMNDPAMNRFD	0	15
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0105	YRAFMNDPAMNRFDV	1	16
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0108	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0108	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0109	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0109	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0111	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0111	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0112	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0112	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0113	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0113	AMNRFDVDNGTSAGR	9	24
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0114	YRAFMNDPAMNRFDV	1	16
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0114	GYRAFMNDPAMNRFD	0	15
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0114	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0213	MNRFDVDNGTSAGRS	10	25
RNF145-PDE4D	chr5	158596687	chr5	58511794	1049	DRB3-0303	MNRFDVDNGTSAGRS	10	25

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Fusion breakpoint peptide structures of RNF145-PDE4D

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
6120	NDPAMNRFDVDNGT	RNF145	PDE4D	chr5	158596687	chr5	58511794	1049

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of RNF145-PDE4D

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	6120	NDPAMNRFDVDNGT	-7.15543	-7.26883
HLA-B14:02	3BVN	6120	NDPAMNRFDVDNGT	-4.77435	-5.80965
HLA-B52:01	3W39	6120	NDPAMNRFDVDNGT	-6.80875	-6.92215
HLA-B52:01	3W39	6120	NDPAMNRFDVDNGT	-4.20386	-5.23916
HLA-A11:01	4UQ2	6120	NDPAMNRFDVDNGT	-7.5194	-8.5547
HLA-A11:01	4UQ2	6120	NDPAMNRFDVDNGT	-6.9601	-7.0735
HLA-A24:02	5HGA	6120	NDPAMNRFDVDNGT	-7.52403	-7.63743
HLA-A24:02	5HGA	6120	NDPAMNRFDVDNGT	-5.82433	-6.85963
HLA-B27:05	6PYJ	6120	NDPAMNRFDVDNGT	-3.28285	-4.31815
HLA-B44:05	3DX8	6120	NDPAMNRFDVDNGT	-5.91172	-6.94702
HLA-B44:05	3DX8	6120	NDPAMNRFDVDNGT	-4.24346	-4.35686

Top

Vaccine Design for the FusionNeoAntigens of RNF145-PDE4D

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
RNF145-PDE4D	chr5	158596687	chr5	58511794	11	20	NRFDVDNGT	GAATCGTTTTGATGTGGACAATGGCAC
RNF145-PDE4D	chr5	158596687	chr5	58511794	11	21	NRFDVDNGTS	GAATCGTTTTGATGTGGACAATGGCACATC
RNF145-PDE4D	chr5	158596687	chr5	58511794	11	22	NRFDVDNGTSA	GAATCGTTTTGATGTGGACAATGGCACATCTGC
RNF145-PDE4D	chr5	158596687	chr5	58511794	3	14	AFMNDPAMNRF	AGCTTTCATGAATGATCCTGCCATGAATCGTTT
RNF145-PDE4D	chr5	158596687	chr5	58511794	4	14	FMNDPAMNRF	TTTCATGAATGATCCTGCCATGAATCGTTT
RNF145-PDE4D	chr5	158596687	chr5	58511794	5	14	MNDPAMNRF	CATGAATGATCCTGCCATGAATCGTTT

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
RNF145-PDE4D	chr5	158595881	chr5	58511794	0	15	DPIVLALGASRDNFD	AGATCCTATTGTTTTGGCACTGGGAGCATCTAGAGACAATTTTGA
RNF145-PDE4D	chr5	158595881	chr5	58511794	10	25	RDNFDVDNGTSAGRS	TAGAGACAATTTTGATGTGGACAATGGCACATCTGCGGGACGGAG
RNF145-PDE4D	chr5	158595881	chr5	58511794	8	23	ASRDNFDVDNGTSAG	AGCATCTAGAGACAATTTTGATGTGGACAATGGCACATCTGCGGG
RNF145-PDE4D	chr5	158595881	chr5	58511794	9	24	SRDNFDVDNGTSAGR	ATCTAGAGACAATTTTGATGTGGACAATGGCACATCTGCGGGACG
RNF145-PDE4D	chr5	158596687	chr5	58511794	0	15	GYRAFMNDPAMNRFD	GGGTTATCGAGCTTTCATGAATGATCCTGCCATGAATCGTTTTGA
RNF145-PDE4D	chr5	158596687	chr5	58511794	1	16	YRAFMNDPAMNRFDV	TTATCGAGCTTTCATGAATGATCCTGCCATGAATCGTTTTGATGT
RNF145-PDE4D	chr5	158596687	chr5	58511794	10	25	MNRFDVDNGTSAGRS	CATGAATCGTTTTGATGTGGACAATGGCACATCTGCGGGACGGAG
RNF145-PDE4D	chr5	158596687	chr5	58511794	2	17	RAFMNDPAMNRFDVD	TCGAGCTTTCATGAATGATCCTGCCATGAATCGTTTTGATGTGGA
RNF145-PDE4D	chr5	158596687	chr5	58511794	8	23	PAMNRFDVDNGTSAG	TCCTGCCATGAATCGTTTTGATGTGGACAATGGCACATCTGCGGG
RNF145-PDE4D	chr5	158596687	chr5	58511794	9	24	AMNRFDVDNGTSAGR	TGCCATGAATCGTTTTGATGTGGACAATGGCACATCTGCGGGACG

Top

Information of the samples that have these potential fusion neoantigens of RNF145-PDE4D

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
SKCM	RNF145-PDE4D	chr5	158596687	ENST00000274542	chr5	58511794	ENST00000340635	TCGA-EE-A2M7-06A

Top

Potential target of CAR-T therapy development for RNF145-PDE4D

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

123_143

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

146_166

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

168_188

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

205_222

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

225_245

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

275_295

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

316_336

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

340_360

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

384_404

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

53_73

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000274542

77_97

401

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

123_143

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

146_166

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

168_188

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

205_222

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

225_245

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

275_295

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

316_336

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

340_360

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

53_73

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000424310

77_97

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

123_143

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

146_166

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

168_188

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

205_222

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

225_245

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

275_295

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

316_336

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

340_360

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

384_404

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

53_73

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000518802

77_97

403

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

123_143

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

146_166

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

168_188

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

205_222

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

225_245

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

275_295

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

316_336

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

340_360

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

53_73

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000519865

77_97

373

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

123_143

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

146_166

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

168_188

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

205_222

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

225_245

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

275_295

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

316_336

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

340_360

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

53_73

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000520638

77_97

387

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

123_143

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

146_166

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

168_188

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

205_222

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

225_245

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

275_295

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

316_336

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

340_360

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

53_73

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158595881

chr5:58511794

ENST00000521606

77_97

390

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

123_143

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

146_166

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

168_188

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

205_222

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

225_245

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

275_295

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

316_336

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

53_73

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000274542

77_97

340

692.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

123_143

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

146_166

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

168_188

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

205_222

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

225_245

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

275_295

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

53_73

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000424310

77_97

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

123_143

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

146_166

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

168_188

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

205_222

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

225_245

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

275_295

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

316_336

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

53_73

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000518802

77_97

342

694.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

123_143

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

146_166

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

168_188

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

205_222

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

225_245

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

275_295

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

53_73

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000519865

77_97

312

664.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

123_143

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

146_166

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

168_188

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

205_222

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

225_245

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

275_295

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

53_73

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000520638

77_97

326

678.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

123_143

329

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

146_166

329

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

168_188

329

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

205_222

329

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

225_245

329

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

275_295

329

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

53_73

329

681.0

Transmembrane

Helical

Hgene

RNF145

chr5:158596687

chr5:58511794

ENST00000521606

77_97

329

681.0

Transmembrane

Helical

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

Top

Related Drugs to RNF145-PDE4D

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to RNF145-PDE4D

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)